FlyBase Gene Report: Dmel\sfl

OTHER GLYCOSYLTRANSFERASES -: The Other Glycosyltransferases (OGTs) group is a collection of glycosyltransferases that do not fit into any of the other major glycosyltransferase groups.
HEPARAN SULFATE SULFOTRANSFERASES -: Heparan sulfate sulfotransferases transfers the sulfo group from 3'-phosphoadenosine 5'-phosphosulfate on to heparan sulfate. (Adapted from FBrf0202009).

Protein Function (UniProtKB)

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in diffusion of morphogen wingless (wg) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of wg morphogens. Required for wg signaling during both embryonic and imaginal disk development. Also required for FGF receptor signaling.

(UniProt, Q9V3L1)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\sfl or the JBrowse view of Dmel\sfl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

sfl-RA

FBtr0076968

NM_079222

5772

1048

sfl-RB

FBtr0301562

NM_001169883

5735

1048

Additional Transcript Data and Comments

Reported size (kB)

5.3 (northern blot)

(Lin and Perrimon, 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

sfl-PA

FBpp0076677

118.6

1048

8.79

NP_523946

AAF50658

sfl-PB

FBpp0290777

118.6

1048

8.79

NP_001163354

ACZ94625

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1048 aa isoforms: sfl-PA, sfl-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

1048 (aa)

(Lin and Perrimon, 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Monomer.

(UniProt, Q9V3L1)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\sfl using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sfl_1

Linkouts

Gene Ontology (20 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

sulfotransferase activity

inferred from mutant phenotype

(assigned by UniProt )

(Toyoda et al., 2000)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

[heparan sulfate]-glucosamine N-sulfotransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000834543

(assigned by GO_Central )

(Gaudet et al., 2011)

deacetylase activity

inferred from biological aspect of ancestor with PANTHER:PTN000834543

(assigned by GO_Central )

(Gaudet et al., 2011)

heparan sulfate N-acetylglucosaminyltransferase activity

inferred from biological aspect of ancestor with PANTHER:PTN000834543

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (13 terms)

Terms Based on Experimental Evidence (13 terms)

CV Term

Evidence

References

epithelial cell migration, open tracheal system

inferred from mutant phenotype

(assigned by UniProt )

(Lin et al., 1999)

inferred from genetic interaction with UniProtKB:Q09147

(assigned by UniProt )

(Lin et al., 1999)

fibroblast growth factor receptor signaling pathway

inferred from mutant phenotype

(assigned by UniProt )

(Lin et al., 1999)

inferred from genetic interaction with UniProtKB:Q07407

(assigned by UniProt )

(Lin et al., 1999)

glycosaminoglycan biosynthetic process

inferred from mutant phenotype

(assigned by UniProt )

(Toyoda et al., 2000)

heparan sulfate proteoglycan biosynthetic process

inferred from mutant phenotype

(Kamimura et al., 2011)

heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process

inferred from mutant phenotype

(assigned by UniProt )

(Toyoda et al., 2000)

imaginal disc-derived wing margin morphogenesis

inferred from mutant phenotype

(Kamimura et al., 2011)

imaginal disc-derived wing vein specification

inferred from mutant phenotype

(Kamimura et al., 2011)

mesoderm migration involved in gastrulation

inferred from mutant phenotype

(assigned by UniProt )

(Lin et al., 1999)

regulation of imaginal disc growth

inferred from mutant phenotype

(Kamimura et al., 2011)

segment polarity determination

inferred from mutant phenotype

(Zhu et al., 2005)

spermatogenesis

inferred from mutant phenotype

(Yu et al., 2015)

sulfur compound metabolic process

inferred from mutant phenotype

(assigned by UniProt )

(Toyoda et al., 2000)

Wnt signaling pathway

inferred from mutant phenotype

(assigned by UniProt )

(Lin and Perrimon, 1999)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process

inferred from biological aspect of ancestor with PANTHER:PTN000834543

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

endoplasmic reticulum

inferred from direct assay

(Yamamoto-Hino et al., 2012)

Golgi cis cisterna

inferred from direct assay

(Yamamoto-Hino et al., 2012)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

Golgi apparatus

inferred from biological aspect of ancestor with PANTHER:PTN000834543

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 4

organism

(Lin and Perrimon, 1999)

Additional Descriptive Data

sfl transcripts are detected in 0-1.5hr embryos on northern blots. Other stages were not tested.

(Lin and Perrimon, 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

endoplasmic reticulum

inferred from direct assay

(Yamamoto-Hino et al., 2012)

Golgi cis cisterna

inferred from direct assay

(Yamamoto-Hino et al., 2012)

Expression Deduced from Reporters

Reporter: P{GawB}sfl^NP2002

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

projection neuron | subset

(Masuda-Nakagawa et al., 2010)

embryonic/larval antennal lobe glomerulus | restricted | subset

(Masuda-Nakagawa et al., 2010)

larval mushroom body calyx glomerulus | restricted | subset

(Masuda-Nakagawa et al., 2010)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\sfl in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\sfl

View exonic expression by tissue for Dmel\sfl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020251

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8339-RA

Flygut - An atlas of the Drosophila adult midgut

FBgn0020251

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of sfl

Transgenic constructs containing regulatory region of sfl

Deletions and Duplications ( 1 )

Disrupted in

Df(3L)ZN47

(BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^αTub84B.PL

sfl^{dsRNA.UAS.THFC}

lethal (with Df(3L)ED211)

sfl^{MI02467-TG4.2}

lethal | embryonic stage | rescuable maternal effect

sfl⁰³⁸⁴⁴

lethal | heat sensitive, with Scer\GAL4^NP5130

sfl^{dsRNA.UAS.THFC}

lethal | heat sensitive, with Scer\GAL4^upd1-E132

sfl^{dsRNA.UAS.THFC}

lethal | recessive

sfl⁰³⁸⁴⁴

lethal - all die before end of pupal stage (with sfl^9B4)

sfl⁰³⁸⁴⁴

lethal - all die before end of pupal stage (with sfl⁰³⁸⁴⁴)

sfl^9B4

partially lethal - majority die, with Scer\GAL4^Act.PU

sfl^UAS.cKa

partially lethal - majority die, with Scer\GAL4^hh.PU

sfl^UAS.cKa

partially lethal - majority die, with Scer\GAL4^pnr-MD237

sfl^GD2336

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

sfl^GD2336

viable, with Scer\GAL4^Mef2.PR

sfl^GD2336

viable, with Scer\GAL4^tin.CΔ4

sfl^GD2336

Sterility

Allele

fertile

sfl^KG10053

male semi-sterile, with Scer\GAL4^NP5130

sfl^{dsRNA.UAS.THFC}

Other Phenotypes

Allele

decreased occurrence of cell division | adult stage, with Scer\GAL4^NP6267

immune response defective | adult stage, with Scer\GAL4^NP6267

sfl^HMS00543

increased mortality during development

sfl⁰³⁸⁴⁴

learning defective | adult stage, with Scer\GAL4^elav.PU

sfl^GD2336

locomotor behavior defective (with sfl^9B4)

sfl⁰³⁸⁴⁴

locomotor behavior defective (with sfl⁰³⁸⁴⁴)

sfl^9B4

locomotor behavior defective | adult stage, with Scer\GAL4^elav.PLu

sfl^GD2336

memory defective | adult stage, with Scer\GAL4^elav.PU

sfl^GD2336

neuroanatomy defective | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

sfl^GD2336

neurophysiology defective (with sfl^9B4)

sfl⁰³⁸⁴⁴

neurophysiology defective (with sfl⁰³⁸⁴⁴)

sfl^9B4

segment polarity phenotype

sfl^unspecified

size defective, with Scer\GAL4^hh.PU

sfl^UAS.cKa

some die during pupal stage, with Scer\GAL4^pnr-MD237

sfl^GD2336

some die during pupal stage (with sfl^9B4)

sfl⁰³⁸⁴⁴

some die during pupal stage (with sfl⁰³⁸⁴⁴)

sfl^9B4

visible, with Scer\GAL4^pnr-MD237

sfl^GD2336

visible | recessive | somatic clone

sfl⁰³⁸⁴⁴

Phenotype manifest in

Allele

adult posterior midgut epithelium, with Scer\GAL4^NP6267

anterior crossvein, with Scer\GAL4^hh.PU

sfl^UAS.cKa

class IV dendritic arborizing neuron | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

sfl^GD2336

cuticle

sfl⁰³⁸⁴⁴

dendritic tree | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

sfl^GD2336

denticle belt

sfl⁰³⁸⁴⁴

distal dendrite | cell non-autonomous | third instar larval stage, with Scer\GAL4^hh-Gal4

sfl^GD2336

elongation stage spermatid, with Scer\GAL4^NP5130

sfl^{dsRNA.UAS.THFC}

embryo

sfl^unspecified

embryonic/first instar larval cuticle

sfl⁰³⁸⁴⁴

embryonic/larval dorsal trunk

sfl⁰³⁸⁴⁴

embryonic/larval ganglionic branch

sfl⁰³⁸⁴⁴

embryonic/larval lateral trunk

sfl⁰³⁸⁴⁴

embryonic/larval tracheal system

sfl⁰³⁸⁴⁴

embryonic/larval tracheal system | germline clone

sfl⁰³⁸⁴⁴

embryonic epidermis

sfl^unspecified

embryonic midgut constriction 2

sfl^unspecified

imaginal tracheoblast

sfl^9B4

mesoderm | germline clone

sfl⁰³⁸⁴⁴

mesothoracic tergum, with Scer\GAL4^pnr-MD237

sfl^GD2336

nucleus, with Scer\GAL4^NP5130

sfl^{dsRNA.UAS.THFC}

sensory mother cell

stomatogastric nervous system

sfl^unspecified

synapse (with sfl^9B4)

sfl⁰³⁸⁴⁴

synapse (with sfl⁰³⁸⁴⁴)

sfl^9B4

testis, with Scer\GAL4^NP5130

sfl^{dsRNA.UAS.THFC}

wing disc, with Scer\GAL4^hh.PU

sfl^UAS.cKa

wing margin, with Scer\GAL4^hh.PU

sfl^UAS.cKa

wing margin | somatic clone

sfl⁰³⁸⁴⁴

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\NDST2

15 of 15

Yes

Hsap\NDST1

14 of 15

Yes

Hsap\NDST4

13 of 15

Yes

Hsap\NDST3

12 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Ndst2

15 of 15

Yes

Mmus\Ndst1

13 of 15

Yes

Mmus\Ndst3

12 of 15

Yes

Mmus\Ndst4

12 of 15

Yes

Rattus norvegicus (Norway rat) (5)

Rnor\Ndst1

12 of 13

Yes

Rnor\Ndst3

12 of 13

Yes

Rnor\Ndst4

12 of 13

Yes

Rnor\NEWGENE_1304700

11 of 13

Yes

Rnor\Ndst2

6 of 13

Yes

Xenopus tropicalis (Western clawed frog) (4)

Xtro\ndst2

12 of 12

Yes

Xtro\ndst4

11 of 12

Yes

Xtro\ndst1

10 of 12

Yes

Xtro\ndst3

7 of 12

Yes

Danio rerio (Zebrafish) (6)

Drer\ndst1a

13 of 15

Yes

Drer\ndst2b

13 of 15

Yes

Drer\ndst2a

12 of 15

Yes

Drer\ndst3

10 of 15

Yes

Drer\ndst1b

5 of 15

Yes

Drer\hic1l

1 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\hst-1

15 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901FK )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\sfl

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21002

Drosophila persimilis

Dper\GL22583

Drosophila willistoni

Dwil\GK12546

Drosophila virilis

Dvir\GJ13376

Drosophila mojavensis

Dmoj\GI12102

Drosophila grimshawi

Dgri\GH15162

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150148 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17007817

Glossina morsitans

Tsetse fly

Gmor\GMOY007792

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_11194

Mayetiola destructor

Hessian fly

Mdes\Mdes004884

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL012539

Anopheles darlingi

American malaria mosquito

Adar\ADAC006344

Anopheles gambiae

Malaria mosquito

Agam\AGAP006328

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ008241

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0161 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000442

Danaus plexippus

Monarch butterfly

Dple\DPOGS215886-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL008595

Heliconius melpomene

Postman butterfly

Hmel\HMEL005325

Apis florea

Little honeybee

Aflo\2239998836

Apis mellifera

Western honey bee

Amel\GB43338

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP11981

Bombus terrestris

Buff-tailed bumblebee

Bter\15213553

Linepithema humile

Argentine ant

Lhum\LH22300

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0024b5

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG009865

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_02025

Tribolium castaneum

Red flour beetle

Tcas\TC013993

Pediculus humanus

Human body louse

Phum\PHUM458660

Rhodnius prolixus

Kissing bug

Rpro\RPRC007629

Cimex lectularius

Bed bug

Clec\CLEC003354

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000523

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI072806

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_11362

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X014A )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR014292

Ixodes scapularis

Black-legged tick

Isca\ISCW023605

Stegodyphus mimosarum

African social velvet spider

Smim\KFM61730.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur04g00950

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_61590

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02CP )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_021604gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Ndst

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000005957

Gallus gallus

Domestic chicken

Ggal\NDST1

Gallus gallus

Domestic chicken

Ggal\NDST4

Gallus gallus

Domestic chicken

Ggal\NDST3

Gallus gallus

Domestic chicken

Ggal\NDST2

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (2)

Gene Symbol

Score

Source

Alignment

2 of 10

2 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

sfl^GD2336

model of intellectual disability

CEA

(Reuter et al., 2014)

sfl^{dsRNA.UAS.THFC}

model of azoospermia

CEA

(Yu et al., 2015)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

sfl^GD2336

exacerbates neonatal diabetes mellitus

modeled by Hsap\INS^C96Y.UAS

(He et al., 2014)

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

NDST2; N-deacetylase and N-sulfotransferase 2

15 of 15

603268

NDST1; N-deacetylase and N-sulfotransferase 1

14 of 15

600853

MENTAL RETARDATION, AUTOSOMAL RECESSIVE 46; MRT46

NDST4; N-deacetylase and N-sulfotransferase 4

13 of 15

615039

NDST3; N-deacetylase and N-sulfotransferase 3

12 of 15

603950

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lin et al., 1999)

suppressible

(Kamimura et al., 2011)

sfl

enhanceable

Hsepi

(Dejima et al., 2013)

sfl

suppressible

Hsepi

(Kamimura et al., 2011)

sfl

suppressible

htl

(Lin et al., 1999)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

suppressible

(Lin et al., 1999)

enhanceable

(Lin and Perrimon, 1999)

Hsepi

enhanceable

sfl

(Dejima et al., 2013)

enhanceable

(Cho et al., 2012)

enhanceable

(Webber et al., 2018)

External Data

Subunit Structure (UniProtKB)

Monomer.

(UniProt, Q9V3L1 )

Linkouts

BioGRID - A database of protein and genetic interactions.

64188

DroID - A comprehensive database of gene and protein interactions.

FBgn0020251

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38736

MIST (genetic) - An integrated Molecular Interaction Database

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

HS-GAG biosynthesis

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-17

Cytogenetic map

65B3-65B4

Sequence location

3L:6,495,680..6,550,104 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

65B3-65B4

Limits computationally determined from genome sequence between P{PZ}l(3)02094⁰²⁰⁹⁴ and P{lacW}l(3)L4060^L4060

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

65C1-65C2

(Lin, 1999.8.4, BDGP Project Members, 1994-1999)

65C1-65C2

(determined by in situ hybridisation)

(Perrimon et al., 1996)

Experimentally Determined Recombination Data

Location

3-17

(FlyBase, 2016)

3-15.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Bloomington

12782

w¹¹¹⁸; P{GT1}sfl^BG02274

50538

y¹ v¹; P{TRiP.GLC01656}attP40

36447

y¹ w^*; Mi{MIC}sfl^MI02467

37008

y¹ w^*; Mi{MIC}sfl^MI03527

16501

y¹; P{SUPor-P}sfl^KG10053 ry⁵⁰⁶

21840

y¹ w^67c23; P{Mae-UAS.6.11}sfl^DP01300

63165

y¹ w^*; Mi{PT-GFSTF.2}sfl^{MI02467-GFSTF.2}

34601

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00543}attP2

67442

y¹ w^*; Mi{Trojan-GAL4.2}sfl^{MI02467-TG4.2}/TM3, Sb¹ Ser¹

Kyoto

104060

w^*; P{GawB}sfl^NP2002 / TM3, Sb¹ Ser¹

VDRC

v330359

P{VSH330359}attP40

v5070

w¹¹¹⁸; P{GD2336}v5070

More stocks available...

Genomic Clones (47)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (31)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

IP02947

Other clones

MIP25166

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020251

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020251

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38736

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: sfl CG8339

(Gabrielian, 1999.11)

Source for database merge of

Source for merge of: sfl l(3)03844

(Lin and Perrimon, 1999)

Additional comments

Release 2 annotation CG8339 consisted of 2 non-overlapping transcripts - CT42364 and CT24567. These have been separated into 2 annotations in release 3 of the genome annotation; release 3 annotation CG32396 contains sequences corresponding to release 2 CT42364 and the release 3 annotation for sfl contains sequences corresponding to CT24567.

(FlyBase Genome Annotators, 2002-2003)

Other Comments

ChEST reveals this is a target of Mef2.

(Junion et al., 2005)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Mutant clones do not show an alteration in the expression of intracellular wg protein but show a dramatic decrease in extracellular wg protein.

(Baeg et al., 2001)

sfl is essential for wg signalling.

(Lin and Perrimon, 1999)

Loss of both maternal and zygotic sfl function results in profound defects in mesoderm and tracheal cell migration in the embryo.

(Lin et al., 1999)

Identification: Defined by a P-element mutation.

(Lin et al., 1997)

Cuticle phenotype similar to that of wg null alleles.

(Lin et al., 1997)

The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(w^hs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

(Perrimon et al., 1996)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 43 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

38736

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q9V3L1

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JID8

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020251

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

sfl_1

Flygut - An atlas of the Drosophila adult midgut

FBgn0020251

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

38736

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC013993

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG8339

modMine - A data warehouse for the modENCODE project

FBgn0020251

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

HS-GAG biosynthesis

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

Q9V3L1

Linkouts

BioGRID - A database of protein and genetic interactions.

64188

DroID - A comprehensive database of gene and protein interactions.

FBgn0020251

DRSC - Results frm RNAi screens

FBgn0020251

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020251

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG8339-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0020251

FlyMine - An integrated database for Drosophila genomics

FBgn0020251

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

38736

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

MIST (genetic) - An integrated Molecular Interaction Database

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

HS-GAG biosynthesis

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG8339

(Meadows, 2017.1.11, Hosono et al., 2015, Yamamoto-Hino et al., 2015, Yamamoto-Hino et al., 2012, Ni et al., 2010.12.1, Quinones-Coello, 2007, Goldstein, 2000.5.31)

CT24567

(Goldstein and Gunawardena, 2000)

NDST

(Kamimura and Maeda, 2017, Giraldez et al., 2002)

Sfl

(Webber et al., 2018, Yamamoto-Hino et al., 2015, Appocher et al., 2014, Chang et al., 2013, Yamamoto-Hino et al., 2012)

(Lin et al., 1997)

l(3)03844

lincRNA.S4253

(FlyBase Genome Annotators, 2014, Young et al., 2012)

sulfateless

(Muha and Müller, 2013, Staudt et al., 2005)

Name Synonyms

Sulfateless

(Yamamoto-Hino et al., 2012)

diet-wingless

(Lin et al., 1997)

sulfateless

(Neto et al., 2016, Kakugawa et al., 2015, Nagarajan et al., 2015, Yu et al., 2015, He et al., 2014, Reuter et al., 2014, Wang et al., 2013, Zhang et al., 2013, Kamimura et al., 2011, Wang et al., 2011, Yan et al., 2010, Hayashi et al., 2009, Bornemann et al., 2008, Staudt et al., 2005, Torroja et al., 2005, Lin et al., 1997)

Secondary FlyBase IDs

FBgn0010820

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (152)

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