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Gene Dmel\ppk

General Information
Symbol	Dmel\ppk	Species	D. melanogaster
Name	pickpocket	Annotation symbol	CG3478
Feature type	protein_coding_gene	FlyBase ID	FBgn0020258
Gene Model Status	Current	Stock availability	2 publicly available
Also Known As	ppk1, dmdNaC1, BG:DS06238.1
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	35B1-35B1	Sequence location	2L:14,378,218..14,381,170 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene pickpocket is referred to in FlyBase by the symbol Dmel\ppk (CG3478, FBgn0020258). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: sodium channel activity. There is experimental evidence that it is involved in the biological process: thermosensory behavior; larval locomotory behavior. 8 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Na+ channel, amiloride-sensitive; Na+ channel, amiloride-sensitive, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed within 18-24 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1630048_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ppk. Gene sequence location is 2L:14378218..14381170.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2012_01	Sequence features BKN25145 HFA03595
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 35B1-35B1   Limits computationally determined from genome sequence between P{lacW}l(2)34Fak00811&P{PZ}wb09437 and P{EP}elBEP2039&P{PZ}osprJ571
Experimentally Determined Cytological Location
Cytogenetic map Notes References 35B1-35B4     (Lingueglia, 1998.1.13) 35A1-35A4     (Adams, 1998.1.15) 35B1-35B4   (determined by in situ hybridisation)   (Darboux et al., 1998) 35A1-35A4   (determined by in situ hybridisation)   (Adams et al., 1998)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\ppk for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) ppk-RA FBtr0080638  NM_057884   2460   606
Additional Transcript Data & Comments
Reported size (kB)	2.7 (northern blot) (Adams et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein ppk-PA   FBpp0080210   69.4   606   4.94     NP_477232   AAF53394
Additional Polypeptide Data & Comments
Reported size (kDa)	606 (aa); 69 (kD predicted) (Adams et al., 1998) 606 (aa) (Darboux et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Na+ channel, amiloride-sensitive (IPR001873) Na+ channel, amiloride-sensitive, conserved site (IPR020903)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF53394   AE003409 AAF44873   AF043263 AAC38823   AY094859 AAM11212   BI213791   BI215387   BI230610   BT044099   GH748571   GH753359   GH755666   GH758152   GH759709   GH770465   GH770853   GH772655   GH773074   GH777959   GH787018   GH788084   GH788219   GH788331   GH795197   GH796187   GH796623   GH796751   GH800513   GH800934   GH803566   GH806191   GH809274   GH810162   GH812364   GH826986   GH828210   GH833083   GH833403   GH839112   GH844988   GH851778   GH855609   GH862028   GH872132   GH872383   GH874229   GH878140   GH879773   GH884545   GH887040   GH894767   GH895982   GH897767   GH901659   GH902367   GH907201   GH910170   GH913409   GH914724   GH920155   GH920368   GH922194   GH924446   GH924616   GH924703   GH927489   GH932809   GH933819   GH935552   GH935828   GH937410   GH937645   Y16225 CAA76124
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O44940 Q7KT94 Q8SX41
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Adams et al., 1998) embryonic stage 17   peripheral nervous system | restricted   (Adams et al., 1998) dendritic arborizing neuron | subset   (Adams et al., 1998) multidendritic neuron | subset   (Adams et al., 1998) larval stage | early   dendritic arborizing neuron | subset   (Adams et al., 1998) multidendritic neuron | subset   (Adams et al., 1998) peripheral nervous system | restricted   (Adams et al., 1998) larval stage     (Adams et al., 1998) adult stage     (Adams et al., 1998) RT-PCR Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage     (Darboux et al., 1998) embryonic stage 17   dendritic arborizing neuron | subset   (Darboux et al., 1998) multidendritic neuron | subset   (Darboux et al., 1998) presumptive embryonic/larval peripheral nervous system | restricted   (Darboux et al., 1998)
Additional Descriptive Data
	Low levels of ppk transcript expression are detected in early embryos using RT-PCR. Levels are higher in late embryos (14-16 hr), and expression is also detected in larvae, pupae, and adults. Using in situ hybridization, a segmentally repeated pattern of expression is detected in a subset of PNS cells during late embryogenesis. Two groups of neurons (dmd5 and v'md5) are stained in thoracic segments, and three groups (dmd6, v'ada, and vmd5) are stained in abdominal segments. The staining neurons belong to the dendritic arborizing subclas of multidentritic neurons. (Darboux et al., 1998) ppk transcript is detected in embryonic, larval, and adult stages. ppk transcript and protein expression is detected in a subset of peripheral neurons in embryos starting at stage 17, and in early larvae. The subset of PNS neurons in which expression is detected are multidendritic neurons of the dendritic arborizing type. In abdominal segments A1-A7, ppk expression is detected in 1 of 6 dmd neurons, in the v'ada neuron, and in 1 of 5 vmd neurons. In thoracic segments, ppk expression is detected in 1 of 6 dmd neurons, and in 1 ofv'md neurons. (Adams et al., 1998)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 17   dendritic arborizing neuron | subset   (Adams et al., 1998) multidendritic neuron | subset   (Adams et al., 1998) peripheral nervous system | restricted   (Adams et al., 1998) larval stage | early   dendritic arborizing neuron | subset   (Adams et al., 1998) multidendritic neuron | subset   (Adams et al., 1998) peripheral nervous system | restricted   (Adams et al., 1998)
Additional Descriptive Data
	ppk transcript and protein expression is detected in a subset of peripheral nervous system neurons in embryos starting at stage 17, and in early larvae. The subset of PNS neurons in which expression is detected are multidendritic neurons of the dendritic arborizing type. In abdominal segments A1-A7, ppk expression is detected in 1 of 6 dmd neurons, in the v\'ada neuron, and in 1 of 5 vmd neurons. In thoracic segments, ppk expression is detected in 1 of 5 dmd neurons, and in 1 of 5 v\'md neurons. (Adams et al., 1998)
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0020258    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0020258 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed within 18-24 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0020258 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr  107 embryo 16-18hr  (297) embryo 18-20hr  (395) embryo 20-22hr  (349) embryo 22-24hr  (441) larva L1  (397) larva L2  (212) larva L3 12hr old  (131) larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0020258 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr  107 embryo 16-18hr  (297) embryo 18-20hr  (395) embryo 20-22hr  (349) embryo 22-24hr  (441) larva L1  (397) larva L2  (212) larva L3 12hr old  131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    9 embryo 04-06hr    21 embryo 06-08hr    6 embryo 08-10hr    5 embryo 10-12hr    5 embryo 12-14hr    13 embryo 14-16hr    107 embryo 16-18hr    297 embryo 18-20hr    395 embryo 20-22hr    349 embryo 22-24hr    441 larva L1    397 larva L2    212 larva L3 12hr old    131 larva L3 puffstage 1-2    53 larva L3 puffstage 3-6    37 larva L3 puffstage 7-9    40 white prepupae new    21 white prepupae 12hr    22 white prepupae 24hr    8 pupae 2d postWPP    18 pupae 3d postWPP    57 pupae 4d postWPP    41 adult male 01day    60 adult male 05day    63 adult male 30day    63 adult female 01day    31 adult female 05day    15 adult female 30day    14 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0020258    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0020258 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0020258 expression level Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0020258 expression level Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    3.15 Larval Midgut    1.9 Larval Hindgut    2.4 Larval Malpighian Tubules    3.6 Larval Fat Body    2.4 Larval Salivary Gland    3.1 Larval Trachea    4.625 Larval Carcass    10.375 Adult Head    4.4 Adult Eye    1.35 Adult Brain    4.8 Adult Thoracic-Abdominal Ganglion    6.9 Adult Crop    5.7 Adult Midgut    1.2 Adult Hindgut    3.3 Adult Malpighian Tubules    2.9 Adult Fat Body    8.4 Adult Salivary Gland    9.3 Adult Heart    4.025 Adult VirginFemale Spermatheca    5.2 Adult InseminatedFemale Spermatheca    5.1 Adult Ovary    0.3 Adult Testis    2.7 Adult Male Accessory Gland    4.2 Adult Carcass    3.8 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    4.4   NA  Eye    1.35   NA  Brain    4.8   3.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.9   NA  Crop    5.7   1.9  Midgut    1.2   2.4  Hindgut    3.3   3.6  Malpighian Tubules    2.9   2.4  Fat Body    8.4   3.1  Salivary Gland    9.3   NA  Heart    4.025   4.625  Trachea    NA   NA  VirginFemale Spermatheca    5.2   NA  InseminatedFemale Spermatheca    5.1   NA  Ovary    0.3   NA  Testis    2.7   NA  Male Accessory Gland    4.2   10.375  Carcass    3.8 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_12
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020258 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3478-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for ppk Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele locomotor behavior defective ppkdsRNA.cAa locomotor behavior defective, with Scer\GAL4ppk.1.9 ppkE145X.Scer\UAS locomotor behavior defective | larval stage, with Scer\GAL4109(2)80 ppkdsRNA.Scer\UAS Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of ppk Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of ppk Class Mutagen Stocks Known lesion ppkJF03250 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ppkKK104185 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes ppkdsRNA.cAa in vitro construct - RNAi 0 Yes ppkdsRNA.cLa in vitro construct - RNAi 0 Yes ppkdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes ppkE145X.Scer\UAS in vitro construct - regulatory fusion 0 Yes ppkGD1142 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes ppkScer\UAS.cAa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)A400 (Ainsley et al., 2003) Df(2L)b88h49 (Ainsley et al., 2003) Df(2L)Exel6035 (Ryder, 2004.4.26) Df(2L)k08712-rv21 (Roote, 2009.11.24)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD1142} NA P{KK104185} NA P{TRiP.JF03250} NA P{UAS-ppk.A} NA P{UAS-ppk.E145X} NA P{UAS-ppk.RNAi} NA characterization construct P{ppk.hs-mCD8.3xEGFP} NA P{ppk.RNAi} NA P{ppk-CD4-tdGFP} NA P{ppk-CD4-tdTom} NA P{ppk-GAL4.1.9} NA PBac{ppk-CD4-GFP.PHzICH} NA PBac{ppk-CD4-tdGFP} NA PBac{ppk-CD4-tdTom} NA PBac{ppk-CD8-GFP.PCH} NA PBac{ppk-CD8-GFP.PEsICH} NA PBac{ppk-CD8-GFP.PHCH} NA PBac{ppk-CD8-GFP.PHCS} NA PBac{ppk-CD8-GFP.PHCT} NA PBac{ppk-CD8-GFP.PHsICH} NA PBac{ppk-CD8-GFP.PHzICH} NA PBac{ppk-CD8-GFP.PHzICS} NA PBac{ppk-CD8-GFP.PsICH} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 2 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
larval locomotory behavior inferred from mutant phenotype (Ainsley et al., 2003, Xu et al., 2004) thermosensory behavior inferred from mutant phenotype (Kim et al., 2002)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function
CV term Evidence References
sodium channel activity inferred from electronic annotation with InterPro:IPR001873, InterPro:IPR020903 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Anderson et al., 1997, Anderson et al., 1997, Ashburner et al., 1999) non-traceable author statement (Lingueglia, 1998.1.13)
Biological Process
CV term Evidence References
sodium ion transport inferred from electronic annotation with InterPro:IPR001873, InterPro:IPR020903 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
membrane inferred from electronic annotation with InterPro:IPR001873, InterPro:IPR020903 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions ppk allele Gene References ppkdsRNA.Scer\UAS Fmr1 (Xu et al., 2004)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0020258
Orthologs
Genome-wide drosophilid orthologs	Dana\GF15015 Dere\GG24259 Dgri\GH20995 Dmoj\GI20631 Dper\GL25771 Dpse\GA17471 Dsec\GM14849 Dsim\GD22009 Dvir\GJ21600 Dwil\GK18092 Dyak\GE25026
Curated drosophilid orthologs	Dpse\GA17471
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_001896617.1 Brugia malayi (roundworm nematode) ENSBTAP00000001283 Bos taurus (bovine) ENSBTAP00000010209 Bos taurus (bovine) CN00546 Caenorhabditis brenneri (small nematode) CN20835 Caenorhabditis brenneri (small nematode) CN30177 Caenorhabditis brenneri (small nematode) ENSCINP00000000995 Ciona intestinalis (sea squirt) ENSCINP00000027146 Ciona intestinalis (sea squirt) ENSCPOP00000000025 Cavia porcellus (guinea pig) ENSGALP00000000498 Gallus gallus (chicken) 156829 Lottia gigantea (owl limpet) ENSMMUP00000014975 Macaca mulatta (rhesus monkey) 104502 Nematostella vectensis (starlet anemone) ENSOANP00000002623 Ornithorynchus anatinus (platypus) ENSOANP00000002700 Ornithorynchus anatinus (platypus) ENSORLP00000003454 Oryzias latipes (japanese killifish) ENSORLP00000008661 Oryzias latipes (japanese killifish) ENSORLP00000021935 Oryzias latipes (japanese killifish) PP12734 Pristionchus pacificus (nematode) ENSTRUP00000004500 Takifugu rubripes (pufferfish Takifugu) ENSTRUP00000010710 Takifugu rubripes (pufferfish Takifugu) ENSTRUP00000042585 Takifugu rubripes (pufferfish Takifugu) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0242115 FBgn0220091 FBgn0208719 FBgn0193422 FBgn0169770 FBgn0077482 FBgn0163355 FBgn0143368 FBgn0143366 FBgn0128457 FBgn0116391 FBgn0092040
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
Bloomington	29571 y1 v1; P{TRiP.JF03250}attP2
VDRC	v108683 P{KK104185}VIE-260B
Genomic Clones ( 2 )
	BACR21J17 BACR37K07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 67 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE19290
Other clones	FI02125
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE14325 RE21774
Other clones	RP003027010 RP003029621 RP003051670 RP003053700 RP003069107 RP003070859 RP003090823 RP003091583 RP003098220 RP003101100 RP003118713 RP003128345 RP003155612 RP003166778 RP003168381 RP003212658 RP003220505 RP003229928 RP003230548 RP003294806 RP003296739 RP003296933 RP003297646 RP003301870 RP003315048 RP003317223 RP003323004 RP003326224 RP003351636 RP003352412 RP003355826 RP003380094 RP003409622 RP003417463 RP003420467 RP003424926 RP003429680 RP003430964 RP003441364 RP003446302 RP003455335 RP003482261 RP003491740 RP003507954 RP003511795 RP003517784 RP003538731 RP003543493 RP003543947 RP003556755 RP003570940 RP003572422 RP003573670 RP003577902 RP003595144 RP003619245 RP003631245 RP003633643 RP003633655 RP003638378 RP003656299 RP003675063 RP003680167
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0020258 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34843
Antibody Information
	polyclonal (Adams et al., 1998)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: ppk CG3478 (Kodira, 1999.11)
Source for database merge of
Additional comments
Other Comments
	Mutant larvae show an increase in crawling speed and an unusual straight path with fewer stops and turns than wild-type larvae. This enhanced locomotion is due to a higher frequency of sustained peristaltic contraction wave cycling and a significant decrease in the pause period between contraction cycles. (Ainsley et al., 2003) ppk is not a vital gene. (Ashburner et al., 1999) ppk has been cloned and characterised. (Adams et al., 1998) ppk has been cloned and sequenced, and its expression pattern has been analysed. (Darboux et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057884 RefSeq (Proteins) • NP_477232 DDBJ / EMBL / GenBank • AAC38823 AAF44873 AAF53394 AAM11212 AE003409 AF043263 AY094859 BI213791 CAA76124 Y16225 Entrez Gene - A searchable database of RefSeq genes. • 34843 UniProtKB/TrEMBL • O44940 Q7KT94 Q8SX41
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Na+ channel, amiloride-sensitive (IPR001873) Na+ channel, amiloride-sensitive, conserved site (IPR020903)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0020258 DRSC - Results from RNAi screens. • FBgn0020258 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020258 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3478-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0020258 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34843 InParanoid A subset of ortholog calls from InParanoid. • XP_001896617.1 Brugia malayi (roundworm nematode) ENSBTAP00000001283 Bos taurus (bovine) ENSBTAP00000010209 Bos taurus (bovine) CN00546 Caenorhabditis brenneri (small nematode) CN20835 Caenorhabditis brenneri (small nematode) CN30177 Caenorhabditis brenneri (small nematode) ENSCINP00000000995 Ciona intestinalis (sea squirt) ENSCINP00000027146 Ciona intestinalis (sea squirt) ENSCPOP00000000025 Cavia porcellus (guinea pig) ENSGALP00000000498 Gallus gallus (chicken) 156829 Lottia gigantea (owl limpet) ENSMMUP00000014975 Macaca mulatta (rhesus monkey) 104502 Nematostella vectensis (starlet anemone) ENSOANP00000002623 Ornithorynchus anatinus (platypus) ENSOANP00000002700 Ornithorynchus anatinus (platypus) ENSORLP00000003454 Oryzias latipes (japanese killifish) ENSORLP00000008661 Oryzias latipes (japanese killifish) ENSORLP00000021935 Oryzias latipes (japanese killifish) PP12734 Pristionchus pacificus (nematode) ENSTRUP00000004500 Takifugu rubripes (pufferfish Takifugu) ENSTRUP00000010710 Takifugu rubripes (pufferfish Takifugu) ENSTRUP00000042585 Takifugu rubripes (pufferfish Takifugu) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /dbzhnsky/pickpocket1.htm modMine - Data generated by the modENCODE project. • FBgn0020258 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0242115 FBgn0220091 FBgn0208719 FBgn0193422 FBgn0169770 FBgn0077482 FBgn0163355 FBgn0143368 FBgn0143366 FBgn0128457 FBgn0116391 FBgn0092040
Synonyms & Secondary IDs ( 15 )
Reported As
Symbol Synonym	BG:DS06238.1 (Ashburner et al., 1999.10.12, Ashburner et al., 1999) CG3478 (Keleman et al., 2009.8.5, Perkins et al., 2009) dmdNaC1 (Lingueglia, 1998.1.13, Waldmann and Lazdunski, 1998, Darboux et al., 1998) dmdNaCl (Ernstrom and Chalfie, 2002) mdNaC1   pkt   PPK (Liu et al., 2003, Motto et al., 1997, Tobin and Bargmann, 2004) ppk (Grueber et al., 2007, Emoto et al., 2006, Jinushi-Nakao et al., 2007, Crozatier and Vincent, 2008, Horner and Wolfner, 2008, Kimura et al., 2006, Yang et al., 2009, Häsemeyer et al., 2009, Lee et al., 2009, Zlatic et al., 2009, Roote, 2009.11.24, Zheng et al., 2008, Shimono et al., 2009, Parrish et al., 2009, Zhong et al., 2010, Ribeiro and Dickson, 2010, Yasunaga et al., 2010, Chen et al., 2010, Schoenmann et al., 2010) ppk1 (Pettus and Johnson, 2005, Ainsley and Johnson, 2005, Ainsley et al., 2003, Wegman et al., 2006, Ainsley et al., 2006, Tobin and Bargmann, 2004, Ainsley et al., 2008) PPK1 (Xu et al., 2004) unnamed (Adams et al., 1996)
Name Synonym	multidendritic neurons sodium channel 1   pickpocket (Anderson et al., 1997, Emoto et al., 2006, Crozatier and Vincent, 2008, Horner and Wolfner, 2008, Zheng et al., 2008, Yang et al., 2009, Häsemeyer et al., 2009, Lee et al., 2009, Parrish et al., 2009, Zhong et al., 2010, Yasunaga et al., 2010, Schoenmann et al., 2010) pickpocket1 (Zhang and Broadie, 2005, Ainsley et al., 2003) Pickpocket1 (Tobin and Bargmann, 2004, Ainsley et al., 2008)
Secondary FlyBase IDs
References ( 75 )
Generate a list of	All references relating to this gene ( 75 )
List References by type	Research paper  ( 33 ) Supplementary material  ( 4 ) Review  ( 11 ) Personal communication to FlyBase  ( 6 ) Abstract  ( 14 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 1 ) Computer file  ( 1 ) Automatic genome annotation  ( 1 )
Recent research papers ( 5 )
	Chen et al., 2010, J. Neurosci. 30(18): 6247--6252 The amiloride-sensitive epithelial Na+ channel PPK28 is essential for drosophila gustatory water reception. [FBrf0210746] Ribeiro and Dickson, 2010, Curr. Biol. 20(11): 1000--1005 Sex peptide receptor and neuronal TOR/S6K signaling modulate nutrient balancing in Drosophila. [FBrf0210987] Schoenmann et al., 2010, J. Neurosci. 30(18): 6375--6386 Axonal degeneration is regulated by the apoptotic machinery or a NAD+-sensitive pathway in insects and mammals. [FBrf0210748] Yasunaga et al., 2010, Dev. Cell 18(4): 621--632 Dendrite reshaping of adult Drosophila sensory neurons requires matrix metalloproteinase-mediated modification of the basement membranes. [FBrf0210695] Zhong et al., 2010, Curr. Biol. 20(5): 429--434 Pickpocket Is a DEG/ENaC Protein Required for Mechanical Nociception in Drosophila Larvae. [FBrf0210201] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...