FlyBase Gene Report: Dmel\mst

General Information

Symbol

Dmel\mst

Species

D. melanogaster

Name

misato

Annotation Symbol

CG1424

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020272

Gene Model Status

Current

Stock Availability

9 publicly available

Gene Snapshot

misato (mst) encodes a conserved protein that acts as a co-factor of the TCP-1 tubulin chaperone complex. It is required for mitotic spindle assembly and viability and for tubulin polymerization and stability. [Date last reviewed: 2019-03-14]

Other Summaries

Alliance
Auto summary
Interactive Fly

Also Known As

LB20, l(1)LB20

Key Links

Genomic Location

Cytogenetic map

20A1-20A1

Sequence location

X:21,388,977..21,391,533 [+]

Recombination map

1-66

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Protein Family (UniProt)

Belongs to the misato family. (O01939)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

Predictions / Assertions

Summaries

Summary (Interactive Fly)

co-factor of the Tubulin Chaperone Protein-1 (TCP-1) complex - plays an essential role in the Tubulin-folding processes required for proper assembly of spindle microtubules - depletion of Misato in the visceral muscle is a model of the pathogenic mechanism for visceral myopathy - mutations in inhibit kinetochore-driven microtubule growth

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\mst or the JBrowse view of Dmel\mst for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

mst-RA

FBtr0077211

NM_078711

1893

574

mst-RB

FBtr0346380

NM_001298589

2443

574

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

mst-PA

FBpp0076907

64.8

574

4.67

NP_523435

AAF50817

mst-PB

FBpp0312074

64.8

574

4.67

NP_001285518

AHN59988

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

574 aa isoforms: mst-PA, mst-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

574 (aa)

(Maleszka et al., 1998, Miklos et al., 1997)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\mst using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mst_1

Linkouts

Gene Ontology (5 terms)

Molecular Function (0 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (0 terms)

Biological Process (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

mitotic sister chromatid segregation

inferred from mutant phenotype

(Miklos et al., 1997)

mitotic spindle assembly

inferred from mutant phenotype

(Mottier-Pavie et al., 2011)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

mitochondrial genome maintenance

inferred from biological aspect of ancestor with PANTHER:PTN000333441

(assigned by GO_Central )

(Gaudet et al., 2011)

mitochondrion distribution

inferred from biological aspect of ancestor with PANTHER:PTN000333441

(assigned by GO_Central )

(Gaudet et al., 2011)

mitotic sister chromatid segregation

inferred from biological aspect of ancestor with PANTHER:PTN000333441

(assigned by GO_Central )

(Gaudet et al., 2011)

mitotic spindle assembly

inferred from biological aspect of ancestor with PANTHER:PTN000333441

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Mottier-Pavie et al., 2011)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000333441

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Maleszka et al., 1998)

larval stage

imaginal disc

(Maleszka et al., 1998)

embryonic/larval fat body

(Maleszka et al., 1998)

adult stage

adult brain

(Maleszka et al., 1998)

adult stage | female

(Miklos et al., 1997)

adult stage & oogenesis

ovary

(Maleszka et al., 1998)

RT-PCR

Stage

Tissue/Position (including subcellular localization)

Reference

pharate adult stage

brain

(Maleszka et al., 1998)

Additional Descriptive Data

mst transcripts are detected in embryos, larvae, pupae, and adults on northern blots and in ovaries, larval fat body, imaginal discs, and late pupal and adult brain by RT-PCR.

(Maleszka et al., 1998)

The mst transcript is relatively rare. The highest levels are found in the female germ line.

(Miklos et al., 1997)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Mottier-Pavie et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\mst in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\mst

View exonic expression by tissue for Dmel\mst

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020272

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1424-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1424

Flygut - An atlas of the Drosophila adult midgut

FBgn0020272

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 17 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 14 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of mst

Transgenic constructs containing regulatory region of mst

Deletions and Duplications ( 157 )

Disrupted in

Df(1)2/19B

(Maleszka et al., 1996, Miklos and de Couet, 1990, Perrimon et al., 1989)

Df(1)14-11

(Munoz, 2003.12.10)

Df(1)16-1-88

(Munoz, 2004.1.5)

Df(1)16-3-22

(Miklos et al., 1987)

(Munoz, 2004.1.5)

(Munoz, 2004.1.5)

(Munoz, 2004.1.5)

(Munoz, 2004.1.5)

(Schalet and Lefevre, 1973)

Df(1)17-241

(Munoz, 2003.12.10)

Df(1)17-244

(Munoz, 2004.1.5)

Df(1)17-257

(Maleszka et al., 1996, Russell et al., 1992, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)17-322

(Munoz, 2004.1.5)

Df(1)17-351

(Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)17-412

(Munoz, 2004.1.5)

Df(1)17-489

(Perrimon et al., 1989, Miklos et al., 1987)

Df(1)18-36

(Munoz, 2004.1.5)

Df(1)19-2

(Munoz, 2004.1.5)

Df(1)19-18

(Munoz, 2004.1.5, Munoz, 2003.12.10)

Df(1)26B

(Perrimon et al., 1989)

Df(1)156

(Paradi et al., 1983)

Df(1)217

(Paradi et al., 1983)

Df(1)218

(Paradi et al., 1983)

Df(1)A53

(Perrimon et al., 1989)

Df(1)B12

(Prud'homme et al., 1995, Lifschytz and Yakobovitz, 1978)

Df(1)C74

(Miklos and de Couet, 1990, Perrimon et al., 1989)

Df(1)D43L1

(Kramers et al., 1991, Perrimon et al., 1989, Kramers et al., 1983, Paradi et al., 1983)

Df(1)DCB1-35b

(Prud'homme et al., 1995, Perrimon et al., 1989)

Df(1)GA33

(Perrimon et al., 1989)

Df(1)GA37

(Perrimon et al., 1989)

Df(1)GA40

(Perrimon et al., 1989)

Df(1)GA104

(Maleszka et al., 1996, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)GE263

(Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)HM45

(Kramers et al., 1983)

Df(1)HM49

(Kramers et al., 1983)

Df(1)HM50

(Kramers et al., 1983)

Df(1)JA117

(Miklos et al., 1997, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)JC77

(Miklos et al., 1997, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)LB6

(Prud'homme et al., 1995, Perrimon et al., 1989)

Df(1)LB7

(Perrimon et al., 1989)

Df(1)LB23

(Perrimon et al., 1989)

Df(1)mal3

(Kramers et al., 1991, Kramers et al., 1983)

Df(1)mal6

(Kramers et al., 1991, Kramers et al., 1983, Paradi et al., 1983)

Df(1)mal12

(Paradi et al., 1983)

Df(1)n17-8

(Munoz, 2004.1.5)

Df(1)N77

(Perrimon et al., 1989)

Df(1)Q539

(Miklos et al., 1997, Prud'homme et al., 1995, Kramers et al., 1991, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987, Kramers et al., 1983, Paradi et al., 1983, Schalet and Lefevre, 1973)

Df(1)S54

(Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)su(f)7085

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)9122

(Lifschytz and Yakobovitz, 1978)

Df(1)T100

(Paradi et al., 1983)

Df(1)T101

(Paradi et al., 1983)

Df(1)T103

(Paradi et al., 1983)

Df(1)T106

(Paradi et al., 1983)

Df(1)T107

(Paradi et al., 1983)

Df(1)T113

(Paradi et al., 1983)

Df(1)T114

(Paradi et al., 1983)

Df(1)T122

(Paradi et al., 1983)

Df(1)T123

(Paradi et al., 1983)

Df(1)T124

(Paradi et al., 1983)

Df(1)T130

(Paradi et al., 1983)

Df(1)T138

(Paradi et al., 1983)

Not disrupted in

Df(1)16-2-13

(Prud'homme et al., 1995, Miklos et al., 1987)

Df(1)16-3-35

(Kramers et al., 1991, Perrimon et al., 1989, Miklos et al., 1987, Paradi et al., 1983)

Df(1)16-3-53

(Munoz, 2004.1.5)

Df(1)16-129

(Maleszka et al., 1996, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987)

Df(1)17-87

(Rahman and Lindsley, 1980)

Df(1)17-137

(Kramers et al., 1991, Schalet and Lefevre, 1973)

Df(1)17-148

(Prud'homme et al., 1995)

(Munoz, 2004.1.5)

(Munoz, 2004.1.5)

(Munoz, 2004.1.5)

(Munoz, 2004.1.5, Schalet and Lefevre, 1973)

Df(1)120

(Paradi et al., 1983)

Df(1)127

(Paradi et al., 1983)

Df(1)136

(Paradi et al., 1983)

Df(1)167

(Paradi et al., 1983)

Df(1)208

(Paradi et al., 1983)

Df(1)216

(Paradi et al., 1983)

Df(1)228

(Paradi et al., 1983)

Df(1)A118

(Kramers et al., 1991, Perrimon et al., 1989, Miklos et al., 1987, Kramers et al., 1983, Paradi et al., 1983, Lifschytz and Yakobovitz, 1978)

Df(1)A209

(Rahman and Lindsley, 1980)

Df(1)B57

(Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987, Lifschytz and Yakobovitz, 1978)

Df(1)DCB1-35c

(Prud'homme et al., 1995, Miklos et al., 1987)

Df(1)EA113

(Miklos et al., 1987)

Df(1)flam-l9

(Prud'homme et al., 1995)

Df(1)GA22

(Miklos et al., 1987)

Df(1)GA42

(Rahman and Lindsley, 1980)

Df(1)GA90

(Rahman and Lindsley, 1980)

Df(1)GA131

(Miklos et al., 1987)

Df(1)HC279

(Perrimon et al., 1989)

Df(1)HF359

(Miklos et al., 1987)

Df(1)HM5

(Kramers et al., 1983)

Df(1)HM8

(Kramers et al., 1983)

Df(1)HM44

(Miklos et al., 1997, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987, Miklos et al., 1986, Kramers et al., 1983)

Df(1)HM47

(Kramers et al., 1983)

Df(1)HM418

(Kramers et al., 1983)

Df(1)HM422

(Kramers et al., 1983)

Df(1)HM444

(Kramers et al., 1983)

Df(1)HM455

(Kramers et al., 1983)

Df(1)JA21

(Miklos et al., 1987)

Df(1)JC4

(Miklos and de Couet, 1990, Miklos et al., 1987)

Df(1)l2

(Prud'homme et al., 1995)

Df(1)l3

(Prud'homme et al., 1995)

Df(1)l4

(Prud'homme et al., 1995)

Df(1)l5

(Prud'homme et al., 1995)

Df(1)l6

(Prud'homme et al., 1995)

Df(1)l7

(Prud'homme et al., 1995)

Df(1)l8

(Prud'homme et al., 1995)

Df(1)l10

(Prud'homme et al., 1995)

Df(1)l11

(Prud'homme et al., 1995)

Df(1)l12

(Prud'homme et al., 1995)

Df(1)l13

(Prud'homme et al., 1995)

Df(1)l14

(Prud'homme et al., 1995)

Df(1)l15

(Prud'homme et al., 1995)

Df(1)l16

(Prud'homme et al., 1995)

Df(1)M31

(Lifschytz and Yakobovitz, 1978)

Df(1)M158

(Lifschytz and Yakobovitz, 1978)

Df(1)M171

(Lifschytz and Yakobovitz, 1978)

Df(1)M176

(Lifschytz and Yakobovitz, 1978)

Df(1)mal8

(Kramers et al., 1991, Paradi et al., 1983)

Df(1)mal10

(Perrimon et al., 1989, Paradi et al., 1983)

Df(1)mal12

(Rahman and Lindsley, 1980)

Df(1)mal17

(Perrimon et al., 1989)

Df(1)R7

(Prud'homme et al., 1995)

Df(1)R20

(Prud'homme et al., 1995)

Df(1)R21

(Prud'homme et al., 1995)

Df(1)R25

(Rahman and Lindsley, 1980)

Df(1)R44

(Prud'homme et al., 1995, Rahman and Lindsley, 1980)

Df(1)R46

(Prud'homme et al., 1995)

Df(1)run1112

(Perrimon et al., 1989)

Df(1)Sp20A-F

(Schalet, 1986)

Df(1)su(f)4B

(Kramers et al., 1991, Kramers et al., 1983)

Df(1)su(f)724

(Prud'homme et al., 1995, Lifschytz and Yakobovitz, 1978)

Df(1)su(f)733

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)754

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)795

(Lifschytz and Yakobovitz, 1978)

Df(1)su(f)7009

(Lifschytz and Yakobovitz, 1978)

Df(1)T2-14A

(Perrimon et al., 1989, Miklos et al., 1987)

Df(1)T105

(Paradi et al., 1983)

Df(1)T110

(Paradi et al., 1983)

Df(1)T136

(Paradi et al., 1983)

Df(1)T137

(Paradi et al., 1983)

Df(1)X-1

(Rahman and Lindsley, 1980)

Df(1)y-X15

(Kramers et al., 1991, Rahman and Lindsley, 1980)

Dp(1;Y)y⁺mal¹²⁶

(Lifschytz and Yakobovitz, 1978)

Not duplicated in

Dp(1;Y)B^S

(Prud'homme et al., 1995, Kramers et al., 1991)

Dp(1;Y)y⁺mal¹¹⁸

(Prud'homme et al., 1995)

Dp(1;Y)y⁺mal¹²⁶

(Kramers et al., 1991, Miklos and de Couet, 1990, Kramers et al., 1983, Schalet and Lefevre, 1973)

Dp(1;Y)y⁺mal^N152

(Prud'homme et al., 1995)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | germline clone

lethal, with Scer\GAL4^da.PU

mst^JF03280

lethal, with Scer\GAL4^Tub.PU

mst^JF03280

lethal | recessive

lethal - all die before end of larval stage | recessive

viable

mst^f07363

Sterility

Allele

fertile

mst^f07363

Other Phenotypes

Allele

feeding behavior defective | adult stage, with Scer\GAL4^Act5C.PI

mst^JF03280

feeding behavior defective | adult stage, with Scer\GAL4^how-24B

mst^JF03280

feeding behavior defective | adult stage, with Scer\GAL4^Mef2.PU

feeding behavior defective | adult stage, with Scer\GAL4^tey-5053A

mst^JF03280

increased cell death | adult stage, with Scer\GAL4^Mef2.PU

mst^JF03280

mitotic cell cycle defective

mitotic cell cycle defective | larval stage

short lived, with Scer\GAL4^how-24B

mst^JF03280

short lived, with Scer\GAL4^Mef2.PU

mst^JF03280

size defective, with Scer\GAL4^how-24B

mst^JF03280

size defective, with Scer\GAL4^Mef2.PU, Scer\GAL80^nSyb.PS

mst^JF03280

size defective, with Scer\GAL4^tey-5053A

mst^JF03280

size defective | adult stage, with Scer\GAL4^Mef2.PU

size defective | adult stage | progressive, with Scer\GAL4^Mef2.PU

mst^JF03280

some die during larval stage | recessive

wild-type

Phenotype manifest in

Allele

adult abdomen, with Scer\GAL4^Mef2.PU

mst^JF03280

adult midgut, with Scer\GAL4^how-24B

mst^JF03280

adult midgut, with Scer\GAL4^Mef2.PU

adult midgut, with Scer\GAL4^Mef2.PU, Scer\GAL80^nSyb.PS

mst^JF03280

adult midgut, with Scer\GAL4^tey-5053A

mst^JF03280

adult midgut longitudinal muscle, with Scer\GAL4^tey-5053A

mst^JF03280

adult midgut muscle, with Scer\GAL4^how-24B

mst^JF03280

adult midgut muscle, with Scer\GAL4^Mef2.PU

filamentous actin | adult stage, with Scer\GAL4^Mef2.PU

mitochondrion | adult stage, with Scer\GAL4^Mef2.PU

mst^JF03280

mitotic spindle

neuroblast | larval stage

sarcomere | adult stage, with Scer\GAL4^Mef2.PU

mst^JF03280

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MSTO1

15 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Msto1

13 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Msto1

13 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\msto1

8 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\msto1

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (0)

No records found.

Arabidopsis thaliana (thale-cress) (1)

Atha\AT4G37190

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\DML1

9 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\dml1

8 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091905GT )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\mst

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA12852

Drosophila persimilis

Dper\GL16019

Drosophila willistoni

Dwil\GK25434

Drosophila virilis

Dvir\GJ16019

Drosophila mojavensis

Dmoj\GI21660

Drosophila grimshawi

Dgri\GH24680

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504JT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17019003

Glossina morsitans

Tsetse fly

Gmor\GMOY003790

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10187

Mayetiola destructor

Hessian fly

Mdes\Mdes005213

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL008947

Anopheles darlingi

American malaria mosquito

Adar\ADAC003063

Anopheles gambiae

Malaria mosquito

Agam\AGAP002778

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ012456

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04DG )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA000446

Danaus plexippus

Monarch butterfly

Dple\DPOGS203266-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL015363

Apis florea

Little honeybee

Aflo\4020252708

Apis mellifera

Western honey bee

Amel\LOC551622

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP22222

Bombus terrestris

Buff-tailed bumblebee

Bter\15212273

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00299c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG004352

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_05413

Tribolium castaneum

Red flour beetle

Tcas\GLEAN_15425

Pediculus humanus

Human body louse

Phum\PHUM371400

Rhodnius prolixus

Kissing bug

Rpro\RPRC002663

Cimex lectularius

Bed bug

Clec\CLEC009021

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI28797

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001528

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_03305

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X04A6 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR012010

Ixodes scapularis

Black-legged tick

Isca\ISCW015167

Stegodyphus mimosarum

African social velvet spider

Smim\KFM62795.1

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_300608

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0FFX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Msto1

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000008620

Gallus gallus

Domestic chicken

Ggal\ENSGALG00000019794

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

visceral myopathy (postulated), MSTO1-related

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 3 )

Allele

Disease

Evidence

References

mst^JF03280

model of congenital myopathy

CEA

(Min et al., 2017)

mst^KK101611

model of congenital myopathy

CEA

(Min et al., 2017)

mst^NIG.1424R

model of congenital myopathy

CEA

(Min et al., 2017)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 2 )

Allele

Disease

Interaction

References

mst^UAS.ORF

ameliorates congenital myopathy

modeled by mst^JF03280

(Min et al., 2017)

mst^JF03280

model of congenital myopathy

is ameliorated by mst^UAS.ORF

(Min et al., 2017)

is ameliorated by Act79B^UASp.GFP

(Min et al., 2017)

is ameliorated by BacA\p35^UAS.cHa

(Min et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

MSTO1; misato mitochondrial distribution and morphology regulator 1

15 of 15

617619

MYOPATHY, MITOCHONDRIAL, AND ATAXIA; MMYAT

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

mst - CCT1

anti tag coimmunoprecipitation, Identification by mass spectrometry, anti bait coimmunoprecipitation, western blot

(Palumbo et al., 2015)

mst - CCT2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CCT3

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CCT4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CCT5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CCT6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CCT7

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CCT8

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - CG5721

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - PDCD-5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - Pfdn1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - Pfdn2

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - Pfdn4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - Pfdn5

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - Pfdn6

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - STUB1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - Smyd4-4

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - mgr

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

mst - viaf

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Palumbo et al., 2015)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

mst

suppressible

Act79B

(Min et al., 2017)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0020272

MIST (protein-protein) - An integrated Molecular Interaction Database

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-66

Cytogenetic map

20A1-20A1

Sequence location

X:21,388,977..21,391,533 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

20A1-20A1

Limits computationally determined from genome sequence between P{EP}CG1702^EP1525 and P{EP}EP1465&P{EP}CG1486^EP1192

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

1-66

(FlyBase, 2016)

1-66.0

(Comeron et al., 2012)

(Miklos, 1997.8.4)

Left of (cM)

(Schalet, 1972)

Right of (cM)

(Schalet, 1972)

Notes

Stocks and Reagents

Stocks (9)

Bloomington

4732

mst³/FM7a

6886

m^s19 mst²/FM6

19072

w¹¹¹⁸ PBac{WH}mst^f07363

29601

y¹ v¹; P{TRiP.JF03280}attP2

52375

y¹ w^* mst^A P{neoFRT}19A/FM7c, P{GAL4-Kr.C}DC1, P{UAS-GFP.S65T}DC5, sn⁺

FlyORF

F001471

M{UAS-mst.ORF}ZH-86Fb

f07363

v330393

v110422

Genomic Clones (10)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (6)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD34822

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020272

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020272

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33119

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mottier-Pavie et al., 2011)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: mst CG1424

(Li, 1999.11)

Source for database merge of

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Origin and Etymology

Discoverer

Etymology

Misato means 'beautiful dawn'.

(Miklos et al., 1997)

Identification

External Crossreferences and Linkouts ( 31 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33119

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O01939

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2JG23

Other crossreferences

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020272

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

mst_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1424

Flygut - An atlas of the Drosophila adult midgut

FBgn0020272

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33119

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1424

modMine - A data warehouse for the modENCODE project

FBgn0020272

Linkouts

DroID - A comprehensive database of gene and protein interactions.

FBgn0020272

DRSC - Results frm RNAi screens

FBgn0020272

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020272

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1424-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0020272

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

MIST (protein-protein) - An integrated Molecular Interaction Database

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

CG1424

(Meadows, 2017.9.14, Schertel et al., 2013, Keleman et al., 2009.8.5, Perkins et al., 2009, Goldstein and Gunawardena, 2000)

LB-20

(Paradi et al., 1983)

LB20

(Maleszka et al., 1996, Prud'homme et al., 1995, Kramers et al., 1991, Miklos and Cotsell, 1990, Miklos and de Couet, 1990, Perrimon et al., 1989, Miklos et al., 1987, Lefevre and Watkins, 1986, Schalet, 1986, Kramers et al., 1983, Lefevre, 1981)

l(1)19Fg

l(1)LB14

(Rahman and Lindsley, 1980)

l(1)LB20

(Paradi et al., 1983, Schalet, 1972)

lLB14

(Schalet and Singer, 1971)

mst

(Min et al., 2017, Baumbach et al., 2015, Schertel et al., 2013, Yamamoto et al., 2013-, Gatti et al., 2012, Japanese National Institute of Genetics, 2012.5.21, Mottier-Pavie et al., 2011, Anaka et al., 2009, Ayroles et al., 2009, Mottier et al., 2008)

Name Synonyms

lethal(1)19Fg

misato

(Mottier-Pavie et al., 2011, Mottier et al., 2008)

Secondary FlyBase IDs

FBgn0001566

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (64)

Report Sections

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