dof, hbr, heartbroken, Downstream of FGF, downstream of FGF receptor
novel signal transduction protein functioning downstream of two FGF receptors - mutations are associated with defects in the migration and later specification of mesodermal and tracheal cells wgvia stabilization of the negative elongation factor complex - promotes anaphase-promoting complex/cyclosome function during cell cycle exit - acts to generate neuroblast cortical polarity - acts to prevent phenotypic variation
Gene model reviewed during 5.47
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 6.03
None of the polypeptides share 100% sequence identity.
1144, 1012 (aa)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\stumps using the Feature Mapper tool.
stumps transcripts are first detected on the ventral side of the embryo in the late syncytial embryo in a region slightly narrower than the mesodermal primordium. During germ band extension, they disappear from the mesoderm. They are observed in tracheal placodes by stage 9/10. As the trachea differentiate, staining disappears in the primary branches and is observed in the secondary branches. stumps transcripts are expressed transiently in the anterior midgut primordium, in parts of the posterior midgut primordium, in a subset of heart cells and in a group of migrating visceral mesoderm cells. Expression is also observed in some glial cells. Later in development expression is observed in parts of the imaginal discs and the brain. The two stumps transcripts appear to be co-expressed everywhere except in the anterior midgut primordium in which only transcript II is expressed.
Transcripts are expressed in ventral cells of the blastoderm embryo. The posterior border coincides with the posterior limit of the ventral furrow. Anteriorly, expression extends up to the anterior pole. Expression continues in the mesoderm. Expression is observed in the tracheal system and in parts of the visceral mesoderm in later embryos.
Prior to stage 11 stumps protein is widely expressed in the embryonic mesoderm. In early stage 11 expression is refined to the dorsal part of the mesoderm and undergoes dynamic modulation in a subset of dorsal cells including the eve-expressing muscle and heart progenitors. stumps protein can also be detected in the ectodermally derived tracheal pits at this stage.
GBrowse - Visual display of RNA-Seq signalsView Dmel\stumps in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Annotations CG18485, CG3375 merged as CG31317 in release 3 of the genome annotation.
stumps function promotes FGF-directed cell migrations, either by potentiating the FGF signalling process or by coupling the signal to the cellular machinery required for directed cell movement.
Mutations in stumps are associated with defects in the migration and later specification of mesodermal and tracheal cells.
stumps is a component of the FGF receptor signaling pathway. It acts downstream of FGF receptors (htl and btl) and upstream of Ras to activate the MAPK kinase cascade in response to FGF signaling, but is not required for activation of the MAPK cascade via other receptor tyrosine kinases.
Mutants exhibit mesodermal spreading (mesodermal cells migrate in an undirected fashion and fail to reach their normal dorsal positions) and cell fate specification loss of function phenotypes.
Isolated from a subtractive cDNA library enriched in sequences expressed in the mesoderm.
The gene was named "stumps" because no tracheal branches form in the mutant, except for an occasional short stump.