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General Information
Symbol
Dmel\dve
Species
D. melanogaster
Name
defective proventriculus
Annotation Symbol
CG5799
Feature Type
FlyBase ID
FBgn0020307
Gene Model Status
Stock Availability
Gene Snapshot
defective proventriculus (dve) encodes a transcriptional repressor that binds to the K50 site. It is involved in developmental patterning, cell-type specification, and functional differentiation. [Date last reviewed: 2019-03-07]
Also Known As

l(2)01738

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:22,243,963..22,286,469 [+]
Recombination map

2-99

RefSeq locus
NT_033778 REGION:22243963..22286469
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (13 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with FLYBASE:vvl; FB:FBgn0086680
traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000387730
(assigned by GO_Central )
Biological Process (9 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
inferred from electronic annotation with InterPro:IPR039673
(assigned by InterPro )
inferred from biological aspect of ancestor with PANTHER:PTN000387730
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000387730
(assigned by GO_Central )
traceable author statement
inferred from sequence or structural similarity with FLYBASE:vvl; FB:FBgn0086680
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000387730
(assigned by GO_Central )
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
CUT HOMEOBOX TRANSCRIPTION FACTORS -
CUT homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. The CUT proteins have a homeodomain and one or more copies of the 75 amino acid DNA-binding CUT domain. (Adapted from FBrf0232555 and PMID:18797923).
Summary (Interactive Fly)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\dve or the JBrowse view of Dmel\dve for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.39

Gene model reviewed during 5.51

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0071775
4903
1024
FBtr0071776
3469
780
Additional Transcript Data and Comments
Reported size (kB)

5.0, 1.7 (northern blot)

4.9 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0071689
112.0
1024
6.64
FBpp0089292
85.8
780
6.40
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

1021, 245 (aa)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dve using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

antennal anlage

Comment: reported as procephalic ectoderm anlage

central brain anlage

Comment: reported as procephalic ectoderm anlage

dorsal head epidermis anlage

Comment: reported as procephalic ectoderm anlage

visual anlage

Comment: reported as procephalic ectoderm anlage

antennal primordium

Comment: reported as procephalic ectoderm primordium

central brain primordium

Comment: reported as procephalic ectoderm primordium

visual primordium

Comment: reported as procephalic ectoderm primordium

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

northern blot
Stage
Tissue/Position (including subcellular localization)
Reference

Comment: reference states 0-18 hr AEL

Additional Descriptive Data

Expression pattern inferred from unspecified enhancer trap line.

Expression is observed in the anterior midline glial cells.

The 5.0 kb dve transcript is expressed at high levels in embryos. Starting in stage 9 embryos, dve transcript is detected in the anterior midgut primordium in a region that will give rise to the proventriculus endoderm. This expression persists and in later stages is confined to the outer endodermal wall of the developing proventriculus. At stage 12, expression is detected at the anterior tip of the posterior midgut primordium, in endodermal cells in the midgut/hindgut boundary, and in cells at the base of the pharynx. At stage 13, expression is detected in the anterior, central and posterior portions of the midgut. In stage 14 embryos, the central expression domain expands, and by stage 16 covers the second and third midgut lobes. Additional expression is detected in mesectodermal cells in stage 9, neural cells in the ventral nerve cord and in the brain lobes at stage 13, and the tip cells of the Malpighian tubules.

The 1.7 kb dve transcript is expressed at low levels in embryos. A probe that detects both the 1.7 and 5.0 kb dve transcripts, and a probe that detects only the 5.0 kb transcript give the same in situ hybridization pattern, indicating that the expression pattern of the 1.7 kb and 5.0 kb transcripts overlap.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

strong dve expression is observed in all outer photoreceptors (R1-R6) from 50% of pupation on. It is absent from R8 and weak expression is observed in a subset of R7 cells. In the adult eye, dve is expressed in yellow R7s but not in pale R7s.

dve expression in wing discs is first seen in late second instar/early third instar as a small group of cells in the center of the rn domain. The dve domain starts earlier and is one or two cells broader than the nab domain but narrower than the rn domain in the presumptive wing. The dve and nab domains are nearly complementary to the zfh2 domain.

dve is expressed in a

reiterated pattern of concentric rings in leg imaginal discs in third instar larvae. At 4 hr APF, dve expression both distal and proximal to the segmental folds. At 6 hr APF, dve is absent in regions proximal to the segmental folds.

dve protein is expressed in the entodermal cells of the developing embryonic foregut. Expression stops sharply at the ectodermal/endodermal boundary.

Protein expression is first detected in the wing disc at the time that wg expression resolves into a peripheral ring and a stripe at the dorsal-ventral boundary. dve expression is observed in a disc-like domain within the ring of wg expression and is excluded from most of the dorsal-ventral boundary soon after expression is initiated.

In early to midthird instar larvae dve protein is detected in the developing wing pouch. Expression initially overlaps that of wg. In mid to late third instar larvae dve protein is excluded from the dorsal/ventral boundary and is expressed in a pattern complementary to wg protein. In late third instar larvae the distal expression of dve is decreased but the proximal expression remains.

dve protein is first detected in the invaginated stomodeum of stage 10 embryos. dve and wg protein expression in the stomodeum overlap at first, but subsequently separate into adjacent but non-overlapping regions. dve protein is expressed in the anterior-most region of the midgut, in the region that will become the outer layer of the proventriculus at stage 16. Starting at stage 13, dve protein is also detected in the central and posterior-most regions of the midgut; the central region, where the anterior and posterior midgut primordia are fused, will become the second and third gut lobes at stage 16. All four types of specialized embryonic/larval midgut cells including copper and interstitial cell precursors in this region express dve at stage 16; in larval stages, dve expression is repressed in copper cells.

Marker for
Subcellular Localization
CV Term
Evidence
References
inferred from direct assay
inferred from direct assay
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\dve in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 13-16
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 39 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of dve
Transgenic constructs containing regulatory region of dve
Deletions and Duplications ( 9 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
7 of 15
Yes
Yes
1  
6 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
7 of 15
Yes
Yes
6 of 15
No
Yes
Rattus norvegicus (Norway rat) (2)
5 of 13
Yes
Yes
5 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (2)
6 of 12
Yes
Yes
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
7 of 15
Yes
Yes
6 of 15
No
Yes
6 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091903NI )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091502GH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W040P )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X03XB )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0GT4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2R
    Recombination map

    2-99

    Cytogenetic map
    Sequence location
    2R:22,243,963..22,286,469 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    58D2-58D2
    Limits computationally determined from genome sequence between P{PZ}ari-207768 and P{lacW}l(2)k09801k09801
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    58D1-58D2
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (21)
    Genomic Clones (48)
    cDNA Clones (66)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequenced
    BDGP DGC clones
    Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    BDGP DGC clones
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of

    Source for identity of: dve CG5799

    Source for database merge of
    Additional comments
    Other Comments

    dve is required for male accessory gland development, both for binucleation of main cells and survival or secondary cells.

    dve isoform-A is important for proventriculus morphogenesis and functional development.

    dve is required at the foregut/midgut boundary for the formation of the proventriculus.

    dve is required for midgut specification under the control of dpp and wg.

    Identified during an enhancer trap screen for flies with an abnormal object fixation response.

    Origin and Etymology
    Discoverer
    Etymology
    Identification
    External Crossreferences and Linkouts ( 57 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    Linkouts
    DPiM - Drosophila Protein interaction map
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Synonyms and Secondary IDs (12)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
    • FBgn0010496
    • FBgn0041142
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (124)