FB2021_06, released December 14, 2021

Gene: Dmel\Rpt6

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General Information
Symbol
Dmel\Rpt6
Species
D. melanogaster
Name
Regulatory particle triple-A ATPase 6
Annotation Symbol
CG1489
Feature Type
protein_coding_gene
FlyBase ID
FBgn0020369
Gene Model Status
Current
Stock Availability
6 publicly available
Enzyme Name (EC)
Proteasome ATPase (5.6.1.5)
Gene Summary
Regulatory particle triple-A ATPase 6 (Rpt6) encodes a subunit of the proteasome, which is responsible for the ATP-dependent degradation of obsolete or damaged proteins. Rpt6 product belongs to the heterohexameric ring formed by triple-A ATPases that unfolds ubiquitinated target proteins before their degradation. [Date last reviewed: 2018-10-04] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Pros45, p42C, DUG

Key Links
Genomic Location
Cytogenetic map
19F4-19F4
Sequence location
Recombination map
1-65
RefSeq locus
NC_004354 REGION:21316052..21317663
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
enables ATP binding
inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables ATP hydrolysis activity
inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables proteasome-activating activity
inferred from biological aspect of ancestor with PANTHER:PTN000553037
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence model
(Wollenberg and Swaffield, 2001)
Biological Process (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Lőw et al., 2013, Wojcik and DeMartino, 2002)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in proteasome-mediated ubiquitin-dependent protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000553037
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred by curator from GO:0005838
(Sumegi et al., 2003)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Mounkes and Fuller, 1998)
located_in nucleus
inferred from direct assay
(Mounkes and Fuller, 1998)
part_of proteasome regulatory particle, base subcomplex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome regulatory particle
inferred from direct assay
(Kiss et al., 2005, Sumegi et al., 2003, Holzl et al., 2000)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of proteasome regulatory particle, base subcomplex
inferred from biological aspect of ancestor with PANTHER:PTN000553037
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity with SGD:S000003016
(Holzl et al., 2000)
part_of proteasome regulatory particle
inferred from sequence or structural similarity with HGNC:9552
(Holzl et al., 2000)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the AAA ATPase family. (O18413)
Protein Signatures (InterPro)
Catalytic Activity (EC)
Experimental Evidence
-
Predictions / Assertions
ATP + H(2)O + polypeptide = ADP + phosphate + unfolded polypeptide (5.6.1.5)
Summaries
Gene Snapshot
Regulatory particle triple-A ATPase 6 (Rpt6) encodes a subunit of the proteasome, which is responsible for the ATP-dependent degradation of obsolete or damaged proteins. Rpt6 product belongs to the heterohexameric ring formed by triple-A ATPases that unfolds ubiquitinated target proteins before their degradation. [Date last reviewed: 2018-10-04]
Gene Group (FlyBase)
PROTEASOME REGULATORY PARTICLE ATPASES -
The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. Within the regulatory particle base, six paralogous AAA-ATPases, termed Rpt1-Rpt6, associate in three pairs (Rpt1-Rpt2, Rpt3-Rpt6, Rpt4-Rpt5), which arrange into a ring to form a trimer of dimers. (Adapted from FBrf0215459 and PMID:28583440.)
Protein Function (UniProtKB)
The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).
(UniProt, O18413)
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\Rpt6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.52

Supported by strand-specific RNA-Seq data.

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Rpt6-RA
FBtr0077189
NM_134603
1404
405
Additional Transcript Data and Comments
Reported size (kB)

1.35 (northern blot)

(Mounkes and Fuller, 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Rpt6-PA
FBpp0076890
45.9
405
8.72
NP_608447
AAF50835
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)

405 (aa)

(Cheng et al., 1998)

405 (aa); 43 (kD observed)

(Mounkes and Fuller, 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with Rpn6 (PubMed:22187461). Interacts with imd and Usp2 isoform A (PubMed:25027767).

(UniProt, O18413)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpt6 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 1 -- 5
organism | ubiquitous
(Cheng et al., 1998)
embryonic stage 4 -- 6
organism
(Fisher et al., 2012)
embryonic stage 5 -- 8
primordial germ cell
(Cheng et al., 1998)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13
anterior midgut primordium
(Cheng et al., 1998)
posterior midgut primordium
(Cheng et al., 1998)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
larval ventral nerve cord
(Fisher et al., 2012)
embryonic stage 16
presumptive embryonic/larval central nervous system
(Cheng et al., 1998)
first instar larval stage
embryonic/larval nervous system
(Cheng et al., 1998)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- pupal stage
(Mounkes and Fuller, 1998)
adult stage | female
(Mounkes and Fuller, 1998)
adult stage | male
(Mounkes and Fuller, 1998)
Additional Descriptive Data

Rpt6 transcripts are uniformly distributed in early embryos. By the cellular blastoderm stage they are located primarily in the pole cells and in a layer between the newly formed epidermal cells and the yolk platelets. At stage 8, expression is mainly in the pole cells and in cells undergoing morphogenetic movement. At stage 13, transcripts are found in the germ band and in the anterior and posterior midgut primordia. In late embryos and in 1st instar larvae, expression is strongest in the CNS.

(Cheng et al., 1998)

Rpt6 transcripts are expressed at all developmental stages and in adult males and females.

(Mounkes and Fuller, 1998)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
western blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
(Mounkes and Fuller, 1998)
Additional Descriptive Data

Rpt6 protein is detected at all developmental stages tested.

(Mounkes and Fuller, 1998)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Mounkes and Fuller, 1998)
located_in nucleus
inferred from direct assay
(Mounkes and Fuller, 1998)
part_of proteasome regulatory particle, base subcomplex
inferred from direct assay
(Nickell et al., 2009)
part_of proteasome regulatory particle
inferred from direct assay
(Kiss et al., 2005, Sumegi et al., 2003, Holzl et al., 2000)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpt6 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 1 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Rpt6
Transgenic constructs containing regulatory region of Rpt6
Aberrations (Deficiencies and Duplications) ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\PSMC5
15 of 15
Yes
Yes
Hsap\PSMC1
2 of 15
No
No
Hsap\PSMC2
2 of 15
No
No
Hsap\ATAD2B
1 of 15
No
Yes
Hsap\ATAD2
1 of 15
No
Yes
Hsap\FGGY
1 of 15
No
No
1  
Hsap\GK2
1 of 15
No
No
Hsap\GK5
1 of 15
No
No
1  
Hsap\GK
1 of 15
No
No
1  
Hsap\KATNA1
1 of 15
No
No
1  
Hsap\KATNAL1
1 of 15
No
No
Hsap\KATNAL2
1 of 15
No
No
Hsap\PSMC3
1 of 15
No
No
Hsap\PSMC4
1 of 15
No
No
Hsap\PSMC6
1 of 15
No
No
Hsap\SHPK
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (16)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Psmc5
15 of 15
Yes
Yes
Mmus\Psmc1
2 of 15
No
No
Mmus\Psmc2
2 of 15
No
No
Mmus\Atad2b
1 of 15
No
Yes
Mmus\Atad2
1 of 15
No
Yes
Mmus\Fggy
1 of 15
No
No
Mmus\Gk2
1 of 15
No
No
Mmus\Gk5
1 of 15
No
No
Mmus\Gk
1 of 15
No
No
Mmus\Gykl1
1 of 15
No
No
Mmus\Katna1
1 of 15
No
No
Mmus\Katnal1
1 of 15
No
No
Mmus\Katnal2
1 of 15
No
No
Mmus\Psmc4
1 of 15
No
No
Mmus\Psmc6
1 of 15
No
No
Mmus\Shpk
1 of 15
No
No
Rattus norvegicus (Norway rat) (17)
Rnor\Psmc5
13 of 13
Yes
Yes
Rnor\Psmc1
2 of 13
No
No
Rnor\Psmc2
2 of 13
No
No
Rnor\Atad2
1 of 13
No
Yes
Rnor\Cyp11a1
1 of 13
No
No
Rnor\Fggy
1 of 13
No
No
Rnor\Gk2
1 of 13
No
No
Rnor\Gk5
1 of 13
No
No
Rnor\Gk
1 of 13
No
No
Rnor\Gykl1
1 of 13
No
No
Rnor\Katna1
1 of 13
No
No
Rnor\Katnal1
1 of 13
No
No
Rnor\Katnal2
1 of 13
No
No
Rnor\LOC103690050
1 of 13
No
No
Rnor\Psmc3
1 of 13
No
No
Rnor\Psmc6
1 of 13
No
No
Rnor\Shpk
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (12)
Xtro\psmc5
9 of 12
Yes
Yes
Xtro\psmc1
2 of 12
No
No
Xtro\psmc2
2 of 12
No
No
Xtro\atad2b
1 of 12
No
Yes
Xtro\atad2
1 of 12
No
Yes
Xtro\fggy
1 of 12
No
No
Xtro\gk
1 of 12
No
No
Xtro\katna1
1 of 12
No
No
Xtro\psmc3
1 of 12
No
No
Xtro\psmc4
1 of 12
No
No
Xtro\psmc6
1 of 12
No
No
Xtro\shpk
1 of 12
No
No
Danio rerio (Zebrafish) (16)
Drer\psmc5
14 of 15
Yes
Yes
Drer\psmc1a
2 of 15
No
No
Drer\psmc1b
2 of 15
No
No
Drer\psmc2
2 of 15
No
No
Drer\atad2b
1 of 15
No
Yes
Drer\atad2
1 of 15
No
Yes
Drer\fggy
1 of 15
No
No
Drer\gk5
1 of 15
No
No
Drer\katna1
1 of 15
No
No
Drer\katnal1
1 of 15
No
No
Drer\katnal2
1 of 15
No
No
Drer\psmc3
1 of 15
No
No
Drer\psmc4
1 of 15
No
No
Drer\psmc6
1 of 15
No
No
Drer\shpk
1 of 15
No
No
Drer\zgc:172295
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (12)
Cele\rpt-6
14 of 15
Yes
Yes
Cele\F56F11.4
13 of 15
No
Yes
Cele\C10G11.8
2 of 15
No
No
Cele\rpt-2
2 of 15
No
No
Cele\C55B6.1
1 of 15
No
No
Cele\lex-1
1 of 15
No
Yes
Cele\mei-1
1 of 15
No
No
Cele\rpt-1
1 of 15
No
No
Cele\rpt-3
1 of 15
No
No
Cele\rpt-4
1 of 15
No
No
Cele\rpt-5
1 of 15
No
No
Cele\T25C8.1
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (11)
Atha\AT5G20000
9 of 9
Yes
Yes
Atha\RPT6A
9 of 9
Yes
Yes
Atha\AT1G45000
1 of 9
No
No
Atha\AT1G53780
1 of 9
No
No
Atha\RPT1A
1 of 9
No
No
Atha\RPT2a
1 of 9
No
No
Atha\RPT2b
1 of 9
No
No
Atha\RPT3
1 of 9
No
No
Atha\RPT4A
1 of 9
No
No
Atha\RPT5A
1 of 9
No
No
Atha\RPT5B
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (7)
Scer\RPT6
14 of 15
Yes
Yes
Scer\GUT1
1 of 15
No
No
Scer\MPA43
1 of 15
No
No
Scer\RPT1
1 of 15
No
No
Scer\RPT2
1 of 15
No
No
Scer\YDR109C
1 of 15
No
No
Scer\YTA7
1 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\rpt6
11 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091909WG )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00004824
Drosophila simulans
Dsim\GD17527
Drosophila sechellia
Dsec\GM23074
Drosophila erecta
Dere\GG19719
Drosophila yakuba
Dyak\GE17917
Drosophila ananassae
Dana\GF21725
Drosophila pseudoobscura pseudoobscura
Dpse\GA13327
Drosophila persimilis
Dper\GL16409
Drosophila willistoni
Dwil\GK16292
Drosophila virilis
Dvir\GJ19383
Drosophila mojavensis
Dmoj\GI14329
Drosophila grimshawi
Dgri\GH11847
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504BU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17024125
Musca domestica
House fly
Mdoa\17013806
Glossina morsitans
Tsetse fly
Gmor\GMOY007591
Glossina morsitans
Tsetse fly
Gmor\GMOY008732
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_10936
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_07625
Mayetiola destructor
Hessian fly
Mdes\Mdes011234
Mayetiola destructor
Hessian fly
Mdes\Mdes009481
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL014723
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL013676
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL005041
Anopheles darlingi
American malaria mosquito
Adar\ADAC009103
Anopheles darlingi
American malaria mosquito
Adar\ADAC000045
Anopheles gambiae
Malaria mosquito
Agam\AGAP007243
Anopheles gambiae
Malaria mosquito
Agam\AGAP007244
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001500
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ009931
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04MA )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA014177
Bombyx mori
Silkmoth
Bmor\BGIBMGA005452
Danaus plexippus
Monarch butterfly
Dple\DPOGS202728-PA
Danaus plexippus
Monarch butterfly
Dple\DPOGS216160-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL016559
Heliconius melpomene
Postman butterfly
Hmel\HMEL012681
Apis florea
Little honeybee
Aflo\3502269956
Apis florea
Little honeybee
Aflo\2187614788
Apis mellifera
Western honey bee
Amel\GB47284
Apis mellifera
Western honey bee
Amel\LOC413404
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17169
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP17263
Bombus terrestris
Buff-tailed bumblebee
Bter\15212496
Bombus terrestris
Buff-tailed bumblebee
Bter\15210364
Linepithema humile
Argentine ant
Lhum\LH10815
Linepithema humile
Argentine ant
Lhum\LH18846
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000f5f
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:00210b
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG005103
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG002531
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10005
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_08999
Tribolium castaneum
Red flour beetle
Tcas\TC013451
Tribolium castaneum
Red flour beetle
Tcas\TC011620
Pediculus humanus
Human body louse
Phum\PHUM086900
Pediculus humanus
Human body louse
Phum\PHUM559310
Rhodnius prolixus
Kissing bug
Rpro\RPRC014950
Rhodnius prolixus
Kissing bug
Rpro\RPRC011257
Cimex lectularius
Bed bug
Clec\CLEC003162
Cimex lectularius
Bed bug
Clec\CLEC009528
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI000649
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006163
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_06641
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_16825
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05BJ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011647
Strigamia maritima
European centipede
Smar\SMAR002216
Strigamia maritima
European centipede
Smar\SMAR000281
Strigamia maritima
European centipede
Smar\SMAR009975
Ixodes scapularis
Black-legged tick
Isca\ISCW009002
Ixodes scapularis
Black-legged tick
Isca\ISCW008741
Stegodyphus mimosarum
African social velvet spider
Smim\KFM56657.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM79432.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM80338.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM60482.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur34g01040
Tetranychus urticae
Two-spotted spider mite
Turt\tetur03g08530
Tetranychus urticae
Two-spotted spider mite
Turt\tetur11g04560
Tetranychus urticae
Two-spotted spider mite
Turt\tetur08g06190
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_52572
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_308117
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_306359
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_323838
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G096W )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_009453gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc6
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_023064gn
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Wdr12
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Psmc5
Ciona intestinalis
Vase tunicate
Cint\LOC100185096
Ciona intestinalis
Vase tunicate
Cint\LOC100187162
Ciona intestinalis
Vase tunicate
Cint\LOC100187378
Ciona intestinalis
Vase tunicate
Cint\LOC100184778
Gallus gallus
Domestic chicken
Ggal\PSMC2
Gallus gallus
Domestic chicken
Ggal\WDR12
Gallus gallus
Domestic chicken
Ggal\PSMC5
Gallus gallus
Domestic chicken
Ggal\PSMC6
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (16)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Rpt6R
7 of 10
Rpt1
4 of 10
Rpt2
4 of 10
Rpt3
3 of 10
Rpt3R
3 of 10
Rpt4
3 of 10
Rpt4R
3 of 10
Rpt5
3 of 10
CG1271
1 of 10
CG8298
1 of 10
CG10793
1 of 10
CG11594
1 of 10
Gk1
1 of 10
Gk2
1 of 10
Kat60
1 of 10
kat-60L1
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    PSMC5; proteasome 26S subunit, ATPase 5
    15 of 15
    601681
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        Rpt6 - Akt
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt6 - Atg6
        two hybrid
        (Hu et al., 2017.6.13)
        Rpt6 - CCT2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CCT3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CCT4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CCT6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CCT8
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CG12321
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CG1416
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CG2064
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - CG33303
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Chc
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Droj2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Dsor1
        anti tag coimmunoprecipitation, peptide massfingerprinting
        (Friedman et al., 2011)
        Rpt6 - GCS2β
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Gfat2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Idh3a
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Ldh
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Mec2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Nfs1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - PSMG1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Pdk1
        anti tag coimmunoprecipitation, Identification by mass spectrometry
        (Vinayagam et al., 2016)
        Rpt6 - Pomp
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosα7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Prosβ7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn10
        molecular sieving, western blot
        (Lipinszki et al., 2009)
        Rpt6 - Rpn11
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn13
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn6
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn7
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn8
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpn9
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpt1
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpt2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpt3
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpt4
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Rpt5
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - ScsβG
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Tapδ
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - UQCR-C2
        experimental knowledge based
        (Guruharsha et al., 2011)
        Rpt6 - Usp2
        anti tag coimmunoprecipitation, anti tag western blot, pull down, peptide massfingerprinting
        (Engel et al., 2014)
        Rpt6 - imd
        anti tag coimmunoprecipitation, anti tag western blot
        (Engel et al., 2014)
        Rpt6 - wal
        experimental knowledge based
        (Guruharsha et al., 2011)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with Rpn6 (PubMed:22187461). Interacts with imd and Usp2 isoform A (PubMed:25027767).
        (UniProt, O18413 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map
        1-65
        Cytogenetic map
        19F4-19F4
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        19F4-19F4
        Limits computationally determined from genome sequence between P{EP}CG1702EP1525 and P{EP}EP1465&P{EP}CG1486EP1192
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        99E-99F
        (Mounkes and Fuller, 1998, Mounkes, 1997.4.14)
        19F-19F
        (Cheng, 1998.1.20)
        19F-19F
        (determined by in situ hybridisation) 99C--99D (determined by in situ hybridisation)
        (Cheng et al., 1998)
        Hybridisation with a genomic clone gives a signal at 19F. Hybridisation with a cDNA clone gives a signal at 19F and (more weakly, but reproducibly) at 99C--99D.
        (Cheng et al., 1998)
        Experimentally Determined Recombination Data
        Location

        1-65

        (FlyBase, 2016)

        1-65.9

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (6)
        Genomic Clones (5)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (75)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Mounkes and Fuller, 1998)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: Pros45 CG1489

        (Li, 1999.11)

        Source for identity of: Rpt6 Pros45

        (FlyBase, 2009-)
        Source for database merge of
        Additional comments

        The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

        (FlyBase, 2009-)

        "DUG" reported in FBrf0101966 and "Pros45" reported in this paper share 99% protein sequence identity and are the same gene (Pros45).

        (Cheng et al., 1998)
        Other Comments

        Expression is enriched in embryonic gonads.

        (Shigenobu et al., 2006)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 88 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (18)
        Reported As
        Symbol Synonym
        1489
        (Ravi et al., 2009)
        CG1489
        (Kolkhof et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Wang et al., 2014, Schertel et al., 2013, Ni et al., 2010.12.1, Ardehali et al., 2009, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Barbash and Lorigan, 2007, Lundgren et al., 2005, Ma et al., 2002)
        DUG
        (Holzl et al., 2000, Mounkes and Fuller, 1998, Mounkes, 1997.4.14)
        Dm_Rpt6a
        (Wollenberg and Swaffield, 2001)
        Dug
        (Ma et al., 2002)
        PRS8_DROME
        (Storm and Sonnhammer, 2003)
        Pros45
        (Zirin et al., 2015, Engel et al., 2014, Bonke et al., 2013, Kwon et al., 2013, Lőw et al., 2013, McPhee et al., 2013, O'Sullivan et al., 2013, Schertel et al., 2013, Davis et al., 2011, Friedman et al., 2011, Toku et al., 2011, Ardehali et al., 2009, Fisher et al., 2008, Hoopfer et al., 2008, Liu and Lehmann, 2008, Barbash and Lorigan, 2007, Shigenobu et al., 2006, Ulvila et al., 2006, Lundgren et al., 2005, Ma et al., 2002)
        Rpt6
        (Aleman et al., 2021, Meltzer et al., 2019, Tsakiri et al., 2019, Blount et al., 2018, Uechi et al., 2018, Hu et al., 2017.6.13, Tain et al., 2017, Transgenic RNAi Project members, 2017-, Wang et al., 2014, Belote and Zhong, 2009, Lipinszki et al., 2009, Nickell et al., 2009, Szabó et al., 2007, Lundgren et al., 2005, Ma et al., 2002)
        S8
        (Lundgren et al., 2005)
        Ug
        dRpt6
        (Wojcik and DeMartino, 2002)
        p42C
        (Lipinszki et al., 2009, Szabó et al., 2007, Kiss et al., 2005, Sumegi et al., 2003, Kurucz et al., 2002, Ma et al., 2002, Holzl et al., 2000)
        pros45
        (Bonke et al., 2013, Cheng, 1998.1.20)
        sug-1
        sug1
        Name Synonyms
        26S proteasome regulatory subunit 8
        (Ulvila et al., 2006)
        Pros45
        (Davis et al., 2011)
        Regulatory particle triple-A ATPase 6
        p42C
        (Fredriksson et al., 2012)
        proteasomal subunit 8
        (Engel et al., 2014)
        Secondary FlyBase IDs
        • FBgn0023422
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (93)