FlyBase Gene Report: Dmel\Rpt6

General Information

Symbol

Dmel\Rpt6

Species

D. melanogaster

Name

Regulatory particle triple-A ATPase 6

Annotation Symbol

CG1489

Feature Type

protein_coding_gene

FlyBase ID

FBgn0020369

Gene Model Status

Current

Stock Availability

7 publicly available

Enzyme Name (EC)

Adenosinetriphosphatase (3.6.1.3)

Gene Snapshot

Regulatory particle triple-A ATPase 6 (Rpt6) encodes a subunit of the proteasome, which is responsible for the ATP-dependent degradation of obsolete or damaged proteins. Rpt6 product belongs to the heterohexameric ring formed by triple-A ATPases that unfolds ubiquitinated target proteins before their degradation. [Date last reviewed: 2018-10-04]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Pros45, p42C, DUG

Key Links

Genomic Location

Cytogenetic map

19F4-19F4

Sequence location

X:21,316,052..21,317,663 [+]

Recombination map

1-65

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

PROTEASOME SUBUNITS

Protein Family (UniProt)

Belongs to the AAA ATPase family. (O18413)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

Predictions / Assertions

ATP binding; ATPase activity; hydrolase activity; TBP-class protein binding

Catalytic Activity (EC)

Experimental Evidence

Predictions / Assertions

ATP + H(2)O = ADP + phosphate (3.6.1.3)

Summaries

Gene Group (FlyBase)

PROTEASOME SUBUNITS -: The 26S proteasome degrades polyubiquitinated proteins in the cytoplasm and nucleus. It is formed from a cylindrical catalytic core 20S proteasome capped at each end by a regulatory 19S particle. The 19S regulatory particle recognizes, unfolds, and translocates ubiquitinated proteins into the catalytic core particle in an ATP-dependent manner. (Adapted from FBrf0215459).

Protein Function (UniProtKB)

The 26S proteasome is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex (By similarity).

(UniProt, O18413)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\Rpt6 or the JBrowse view of Dmel\Rpt6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.52

Supported by strand-specific RNA-Seq data.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Rpt6-RA

FBtr0077189

NM_134603

1404

405

Additional Transcript Data and Comments

Reported size (kB)

1.35 (northern blot)

(Mounkes and Fuller, 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Rpt6-PA

FBpp0076890

45.9

405

8.72

NP_608447

AAF50835

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

405 (aa)

(Cheng et al., 1998)

405 (aa); 43 (kD observed)

(Mounkes and Fuller, 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the 19S proteasome regulatory particle complex. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. Interacts with Rpn6 (PubMed:22187461). Interacts with imd and Usp2 isoform A (PubMed:25027767).

(UniProt, O18413)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

O18413

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rpt6 using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Gene Ontology (14 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

ATP binding

inferred from electronic annotation with InterPro:IPR003959, InterPro:IPR003960

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

ATPase activity

inferred from sequence model

(Wollenberg and Swaffield, 2001)

hydrolase activity

inferred from electronic annotation with InterPro:IPR005937

(assigned by InterPro )

(FlyBase Curators et al., 2004-)

TBP-class protein binding

inferred from biological aspect of ancestor with PANTHER:PTN000553334

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

proteasome-mediated ubiquitin-dependent protein catabolic process

inferred from mutant phenotype

(Lőw et al., 2013, Wojcik and DeMartino, 2002)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

modulation of chemical synaptic transmission

inferred from biological aspect of ancestor with PANTHER:PTN000553337

(assigned by GO_Central )

(Gaudet et al., 2011)

positive regulation of RNA polymerase II transcriptional preinitiation complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN000553334

(assigned by GO_Central )

(Gaudet et al., 2011)

proteasome-mediated ubiquitin-dependent protein catabolic process

inferred by curator from GO:0005838

(Sumegi et al., 2003)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Mounkes and Fuller, 1998)

nucleus

inferred from direct assay

(Mounkes and Fuller, 1998)

proteasome regulatory particle

inferred from direct assay

(Kiss et al., 2005, Sumegi et al., 2003, Holzl et al., 2000)

proteasome regulatory particle, base subcomplex

inferred from direct assay

(Nickell et al., 2009)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

cytosolic proteasome complex

inferred from biological aspect of ancestor with PANTHER:PTN000553334

(assigned by GO_Central )

(Gaudet et al., 2011)

nuclear proteasome complex

inferred from biological aspect of ancestor with PANTHER:PTN000553334

(assigned by GO_Central )

(Gaudet et al., 2011)

postsynapse

inferred from biological aspect of ancestor with PANTHER:PTN000553337

(assigned by GO_Central )

(Gaudet et al., 2011)

proteasome regulatory particle

inferred from sequence or structural similarity with HGNC:9552

(Holzl et al., 2000)

proteasome regulatory particle, base subcomplex

inferred from sequence or structural similarity with SGD:S000003016

(Holzl et al., 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000553334

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 1 -- 5

organism | ubiquitous

(Cheng et al., 1998)

embryonic stage 4 -- 6

organism

(Fisher et al., 2012)

embryonic stage 5 -- 8

pole cell

(Cheng et al., 1998)

embryonic stage 7 -- 8

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 13

anterior midgut primordium

(Cheng et al., 1998)

posterior midgut primordium

(Cheng et al., 1998)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

embryonic stage 16

presumptive embryonic/larval central nervous system

(Cheng et al., 1998)

first instar larval stage

embryonic/larval nervous system

(Cheng et al., 1998)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- pupal stage

(Mounkes and Fuller, 1998)

adult stage | female

(Mounkes and Fuller, 1998)

adult stage | male

(Mounkes and Fuller, 1998)

Additional Descriptive Data

Rpt6 transcripts are uniformly distributed in early embryos. By the cellular blastoderm stage they are located primarily in the pole cells and in a layer between the newly formed epidermal cells and the yolk platelets. At stage 8, expression is mainly in the pole cells and in cells undergoing morphogenetic movement. At stage 13, transcripts are found in the germ band and in the anterior and posterior midgut primordia. In late embryos and in 1st instar larvae, expression is strongest in the CNS.

(Cheng et al., 1998)

Rpt6 transcripts are expressed at all developmental stages and in adult males and females.

(Mounkes and Fuller, 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Mounkes and Fuller, 1998)

Additional Descriptive Data

Rpt6 protein is detected at all developmental stages tested.

(Mounkes and Fuller, 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Mounkes and Fuller, 1998)

nucleus

inferred from direct assay

(Mounkes and Fuller, 1998)

proteasome regulatory particle

inferred from direct assay

(Kiss et al., 2005, Sumegi et al., 2003, Holzl et al., 2000)

proteasome regulatory particle, base subcomplex

inferred from direct assay

(Nickell et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Rpt6 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Rpt6

View exonic expression by tissue for Dmel\Rpt6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020369

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020369

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1489-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1489

Flygut - An atlas of the Drosophila adult midgut

FBgn0020369

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 1 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 7 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Rpt6

Transgenic constructs containing regulatory region of Rpt6

Deletions and Duplications ( 0 )

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal, with Scer\GAL4^pnr-MD237

Rpt6^GD8479

lethal | pupal stage, with Scer\GAL4^pnr-MD237

Rpt6^GD8479

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

Rpt6^GD16791

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

viable, with Scer\GAL4^prd.RG1

Rpt6^UAS.cCa

viable, with Scer\GAL4^tin.CΔ4

Sterility

Allele

fertile, with Scer\GAL4^prd.RG1

Rpt6^UAS.cCa

Other Phenotypes

Allele

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

Rpt6^GD8479

some die during pupal stage, with Scer\GAL4^pnr-MD237

Rpt6^GD16791

Phenotype manifest in

Allele

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\PSMC5

15 of 15

Yes

Hsap\PSMC2

1 of 15

Hsap\PSMC6

1 of 15

Hsap\WDR12

1 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (4)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Psmc5

14 of 15

Yes

Mmus\Psmc2

1 of 15

Mmus\Psmc6

1 of 15

Mmus\Wdr12

1 of 15

Rattus norvegicus (Norway rat) (3)

Rnor\Psmc5

13 of 13

Yes

Rnor\Psmc6

1 of 13

Rnor\Wdr12

1 of 13

Xenopus tropicalis (Western clawed frog) (2)

Xtro\psmc5

8 of 12

Yes

Xtro\wdr12

1 of 12

Danio rerio (Zebrafish) (4)

Drer\psmc5

14 of 15

Yes

Drer\psmc2

1 of 15

Drer\psmc6

1 of 15

Drer\wdr12

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (5)

Cele\F56F11.4

13 of 15

Yes

Cele\rpt-6

13 of 15

Yes

Cele\rpt-1

1 of 15

Cele\rpt-4

1 of 15

Cele\wdr-12

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\AT5G20000

9 of 9

Yes

Atha\RPT6A

9 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\RPT6

15 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\rpt6

12 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091909WG )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\Rpt6

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA13327

Drosophila persimilis

Dper\GL16409

Drosophila willistoni

Dwil\GK16292

Drosophila virilis

Dvir\GJ19383

Drosophila mojavensis

Dmoj\GI14329

Drosophila grimshawi

Dgri\GH11847

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504BU )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17024125

Musca domestica

House fly

Mdoa\17013806

Glossina morsitans

Tsetse fly

Gmor\GMOY007591

Glossina morsitans

Tsetse fly

Gmor\GMOY008732

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_10936

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07625

Mayetiola destructor

Hessian fly

Mdes\Mdes011234

Mayetiola destructor

Hessian fly

Mdes\Mdes009481

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL014723

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL013676

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL005041

Anopheles darlingi

American malaria mosquito

Adar\ADAC009103

Anopheles darlingi

American malaria mosquito

Adar\ADAC000045

Anopheles gambiae

Malaria mosquito

Agam\AGAP007243

Anopheles gambiae

Malaria mosquito

Agam\AGAP007244

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001500

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ009931

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04MA )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA014177

Bombyx mori

Silkmoth

Bmor\BGIBMGA005452

Danaus plexippus

Monarch butterfly

Dple\DPOGS202728-PA

Danaus plexippus

Monarch butterfly

Dple\DPOGS216160-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL016559

Heliconius melpomene

Postman butterfly

Hmel\HMEL012681

Apis florea

Little honeybee

Aflo\3502269956

Apis florea

Little honeybee

Aflo\2187614788

Apis mellifera

Western honey bee

Amel\GB47284

Apis mellifera

Western honey bee

Amel\LOC413404

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP17169

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP17263

Bombus terrestris

Buff-tailed bumblebee

Bter\15212496

Bombus terrestris

Buff-tailed bumblebee

Bter\15210364

Linepithema humile

Argentine ant

Lhum\LH10815

Linepithema humile

Argentine ant

Lhum\LH18846

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000f5f

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00210b

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG005103

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002531

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_10005

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_08999

Tribolium castaneum

Red flour beetle

Tcas\TC013451

Tribolium castaneum

Red flour beetle

Tcas\TC011620

Pediculus humanus

Human body louse

Phum\PHUM086900

Pediculus humanus

Human body louse

Phum\PHUM559310

Rhodnius prolixus

Kissing bug

Rpro\RPRC014950

Rhodnius prolixus

Kissing bug

Rpro\RPRC011257

Cimex lectularius

Bed bug

Clec\CLEC003162

Cimex lectularius

Bed bug

Clec\CLEC009528

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI000649

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006163

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_06641

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_16825

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X05BJ )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR011647

Strigamia maritima

European centipede

Smar\SMAR002216

Strigamia maritima

European centipede

Smar\SMAR000281

Strigamia maritima

European centipede

Smar\SMAR009975

Ixodes scapularis

Black-legged tick

Isca\ISCW009002

Ixodes scapularis

Black-legged tick

Isca\ISCW008741

Stegodyphus mimosarum

African social velvet spider

Smim\KFM56657.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM79432.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM80338.1

Stegodyphus mimosarum

African social velvet spider

Smim\KFM60482.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur34g01040

Tetranychus urticae

Two-spotted spider mite

Turt\tetur03g08530

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g04560

Tetranychus urticae

Two-spotted spider mite

Turt\tetur08g06190

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_52572

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_308117

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_306359

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_323838

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G096W )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_009453gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psmc6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psmc2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psmc

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_023064gn

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Wdr12

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Psmc5

Ciona intestinalis

Vase tunicate

Cint\LOC100185096

Ciona intestinalis

Vase tunicate

Cint\LOC100187162

Ciona intestinalis

Vase tunicate

Cint\LOC100187378

Ciona intestinalis

Vase tunicate

Cint\LOC100184778

Gallus gallus

Domestic chicken

Ggal\PSMC2

Gallus gallus

Domestic chicken

Ggal\WDR12

Gallus gallus

Domestic chicken

Ggal\PSMC5

Gallus gallus

Domestic chicken

Ggal\PSMC6

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (9)

Gene Symbol

Score

Source

Alignment

7 of 10

2 of 10

2 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PSMC5; proteasome 26S subunit, ATPase 5

15 of 15

601681

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Rpt6 - Akt1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rpt6 - Atg6

two hybrid

(Hu et al., 2017.6.13)

Rpt6 - CCT2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CCT3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CCT4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CCT6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CCT8

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CG12321

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CG1416

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CG2064

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - CG33303

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Chc

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Droj2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Dsor1

anti tag coimmunoprecipitation, peptide massfingerprinting

(Friedman et al., 2011)

Rpt6 - GCS2β

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Gfat2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Idh3a

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Ldh

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Mec2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Nfs1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - PSMG1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Pdk1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

Rpt6 - Pomp

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosα7

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Prosβ7

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn10

molecular sieving, western blot

(Lipinszki et al., 2009)

Rpt6 - Rpn11

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn13

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn6

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn7

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn8

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpn9

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpt1

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpt2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpt3

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpt4

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Rpt5

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - ScsβG

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Tapδ

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - UQCR-C2

experimental knowledge based

(Guruharsha et al., 2011)

Rpt6 - Usp2

pull down, peptide massfingerprinting, anti tag coimmunoprecipitation, anti tag western blot

(Engel et al., 2014)

Rpt6 - imd

anti tag coimmunoprecipitation, anti tag western blot

(Engel et al., 2014)

Rpt6 - wal

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

(UniProt, O18413 )

Linkouts

BioGRID - A database of protein and genetic interactions.

59383

DroID - A comprehensive database of gene and protein interactions.

FBgn0020369

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33105

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpt6

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-65

Cytogenetic map

19F4-19F4

Sequence location

X:21,316,052..21,317,663 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

19F4-19F4

Limits computationally determined from genome sequence between P{EP}CG1702^EP1525 and P{EP}EP1465&P{EP}CG1486^EP1192

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

99E-99F

(Mounkes and Fuller, 1998, Mounkes, 1997.4.14)

19F-19F

(Cheng, 1998.1.20)

19F-19F

(determined by in situ hybridisation) 99C--99D (determined by in situ hybridisation)

(Cheng et al., 1998)

Hybridisation with a genomic clone gives a signal at 19F. Hybridisation with a cDNA clone gives a signal at 19F and (more weakly, but reproducibly) at 99C--99D.

(Cheng et al., 1998)

Experimentally Determined Recombination Data

Location

1-65

(FlyBase, 2016)

1-65.9

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (7)

Bloomington

81485

y¹ v¹; P{TKO.GS04815}attP40

33469

w^* P{EP}Rpt6^G1225 CG1801^G1225/FM7h

34712

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01191}attP2

FlyORF

F001611

M{UAS-Rpt6.ORF}ZH-86Fb

VDRC

v100620

P{KK108783}VIE-260B

v24936

w¹¹¹⁸; P{GD8479}v24936

v46803

w¹¹¹⁸; P{GD16791}v46803

Genomic Clones (5)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (75)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

LD26005

Other clones

DPiM_LD26005

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0020369

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0020369

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33105

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Mounkes and Fuller, 1998)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Pros45 CG1489

(Li, 1999.11)

Source for identity of: Rpt6 Pros45

(FlyBase, 2009-)

Source for database merge of

Additional comments

The nomenclature of genes encoding subunits of the 26S proteasome of D. melanogaster have been standardized according to FBrf0215459. These symbols/names largely follow those used already in FlyBase, and largely mirror fly community usage. HOWEVER, note that at least one other nomenclature system exists that is followed by the HUGO Gene Nomenclature Committee (HGNC), for example, with the unfortunate result that several D. melanogaster genes have shared synonyms.

(FlyBase, 2009-)

"DUG" reported in FBrf0101966 and "Pros45" reported in this paper share 99% protein sequence identity and are the same gene (Pros45).

(Cheng et al., 1998)

Other Comments

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 131 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

33105

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O18413

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0020369

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0020369

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1489

Flygut - An atlas of the Drosophila adult midgut

FBgn0020369

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

98956

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

33105

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1489

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

modMine - A data warehouse for the modENCODE project

FBgn0020369

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Linkouts

BioGRID - A database of protein and genetic interactions.

59383

DPiM - Drosophila Protein interaction map

FBgn0020369

DroID - A comprehensive database of gene and protein interactions.

FBgn0020369

DRSC - Results frm RNAi screens

FBgn0020369

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0020369

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1489-RA

FlyMine - An integrated database for Drosophila genomics

FBgn0020369

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

33105

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Proteasome

MIST (protein-protein) - An integrated Molecular Interaction Database

Rpt6

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

1489

(Ravi et al., 2009)

CG1489

(Kolkhof et al., 2017, Çiçek et al., 2016, Hosono et al., 2015, Rosa-Ferreira et al., 2015, Wang et al., 2014, Schertel et al., 2013, Ni et al., 2010.12.1, Ardehali et al., 2009, Belote and Zhong, 2009, Keleman et al., 2009.8.5, Hughes et al., 2008, Barbash and Lorigan, 2007, Lundgren et al., 2005, Ma et al., 2002)

DUG

(Holzl et al., 2000, Mounkes and Fuller, 1998, Mounkes, 1997.4.14)

Dm_Rpt6a

(Wollenberg and Swaffield, 2001)

Dug

(Ma et al., 2002)

PRS8_DROME

(Storm and Sonnhammer, 2003)

Rpt6

(Meltzer et al., 2019, Uechi et al., 2018, Hu et al., 2017.6.13, Transgenic RNAi Project members, 2017-, Wang et al., 2014, Belote and Zhong, 2009, Lipinszki et al., 2009, Nickell et al., 2009, Szabó et al., 2007, Lundgren et al., 2005, Ma et al., 2002)

(Lundgren et al., 2005)

dRpt6

(Wojcik and DeMartino, 2002)

p42C

(Lipinszki et al., 2009, Szabó et al., 2007, Kiss et al., 2005, Sumegi et al., 2003, Kurucz et al., 2002, Ma et al., 2002, Holzl et al., 2000)

pros45

(Bonke et al., 2013, Cheng, 1998.1.20)

sug-1

sug1

Name Synonyms

26S proteasome regulatory subunit 8

(Ulvila et al., 2006)

Pros45

(Davis et al., 2011)

Regulatory particle triple-A ATPase 6

p42C

(Fredriksson et al., 2012)

proteasomal subunit 8

(Engel et al., 2014)

Secondary FlyBase IDs

FBgn0023422

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (88)

Report Sections

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