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Gene Dmel\I-element\RTase

General Information
Symbol	Dmel\I-element\RTase	Species	D. melanogaster
Name	I element reverse transcriptase	Annotation symbol
Feature type	transposable_element_gene	FlyBase ID	FBgn0020418
Gene Model Status	Not Applicable	Stock availability	None publicly available
Also Known As	ORF2
Genomic Location
Chromosome (arm)		Recombination map
Cytogenetic map		Sequence location
Summary Information
Automatically generated summary See sections below for more information	The gene I element reverse transcriptase is referred to in FlyBase by the symbol I-element\RTase (FBgn0020418). It is a transposable_element_gene from I element (D. melanogaster). It is encoded by the I-element transposable element. An electronic pipeline based on InterPro domains suggests that it has the molecular function: RNA binding; RNA-directed DNA polymerase activity; ribonuclease H activity. An electronic pipeline based on InterPro domains suggests that it is involved in the biological process: RNA-dependent DNA replication. 52 alleles are reported. No phenotypic data is available. It has no annotated transcripts. Protein features are: Endonuclease/exonuclease/phosphatase; Reverse transcriptase; Ribonuclease H domain; Ribonuclease H-like domain. Gene has not been localized to the genome sequence.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Reverse transcriptase (IPR000477) Endonuclease/exonuclease/phosphatase (IPR005135) Ribonuclease H-like domain (IPR012337) Ribonuclease H domain (IPR002156)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name M14954 AAA70222
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q24363
Mapped Features
Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
modENCODE Anatomy RNA-Seq
modENCODE Development RNA-Seq
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
Expression Clusters
External Data & Images
Linkouts
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of I-element\RTase Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 52 )
For All Alleles Carried on Transgenic Constructs Show
Allele of I-element\RTase Class Mutagen Stocks Known lesion I-element\RTasea.i1-2Δ:AS.hs in vitro construct - regulatory fusion 0 Yes I-element\RTaseAct5C.PJ in vitro construct - regulatory fusion 0 Yes I-element\RTasefl.cJa in vitro construct 0 Yes I-element\RTasefl.cSa in vitro construct 0 Yes I-element\RTasefs02.T:Ivir\HA1 0 Yes I-element\RTaseHN.hs in vitro construct - regulatory fusion 0 Yes I-element\RTaseHR.hs in vitro construct - regulatory fusion 0 Yes I-element\RTasehs.6.T:MS2\MCP.BS in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes I-element\RTasehs.AAG.T:Ecol\lacZ in vitro construct - deletion 0 Yes I-element\RTasehs.ATG.T:Ecol\lacZ in vitro construct - deletion 0 Yes I-element\RTasehs.ORF1.AAG.T:Ecol\lacZ in vitro construct - site directed mutagenesis 0 Yes I-element\RTasehs.ORF1.ATG.T:Ecol\lacZ in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes I-element\RTaseI-ΔA in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔB in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔCl18 in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔCl21 in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔCl3 in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔD in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔE in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔHind in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔHp-Sn in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔNuc in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTaseI-ΔXba in vitro construct - deletion in vitro construct - minigene 0 Yes I-element\RTasei1-2Δ.hs in vitro construct - regulatory fusion 0 Yes I-element\RTasei1-2Δ.pA in vitro construct 0 Yes I-element\RTasei1-2Δ:S.hs in vitro construct 0 Yes I-element\RTasei2Δ.hs in vitro construct - regulatory fusion 0 Yes I-element\RTasei2Δmut.hs in vitro construct - site directed mutagenesis 0 Yes I-element\RTaseI403I404 in vitro construct - deletion 0 Yes I-element\RTaseILS.Scer\UAS.P\T.T:Avic\GFP in vitro construct - coding region fusion 0 Yes I-element\RTaseIR3 in vitro construct 0 Yes I-element\RTaseIviP2 in vitro construct - genomic fragment 0 Yes I-element\RTaseIyC1 in vitro construct - genomic fragment 0 Yes I-element\RTaseIΔH-EBm in vitro construct 0 Yes I-element\RTaseIΔH-EDm in vitro construct 0 Yes I-element\RTaseIΔH-lacZ in vitro construct 0 Yes I-element\RTaseIΔH-O1Dm in vitro construct 0 Yes I-element\RTaseIΔH-O2Dm in vitro construct 0 Yes I-element\RTaseIΔH in vitro construct - deletion 0 Yes I-element\RTaseIΔNuc in vitro construct - deletion 0 Yes I-element\RTaseLoc+ in vitro construct 0 Yes I-element\RTaseLoc+A in vitro construct 0 Yes I-element\RTaseLoc+AP in vitro construct 0 Yes I-element\RTaseLoc+APM in vitro construct 0 Yes I-element\RTaseLoc+L in vitro construct 0 Yes I-element\RTaseLoc+Mid in vitro construct 0 Yes I-element\RTaseLoc+P.ILS in vitro construct 0 Yes I-element\RTaseLoc+PM in vitro construct 0 Yes I-element\RTaseLoc+R in vitro construct 0 Yes I-element\RTaseΔnuc in vitro construct - deletion 0 Yes I-element\RTaseΔZ in vitro construct 0 Yes I-element\RTaseπI in vitro construct 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{UASp-ILS.GFP} NA heat-shock construct P{hs-I-element-(ms2).6} NA P{i1-2Δ/AS} NA characterization construct P{hs-I-element\RTase.HN} NA P{hs-I-element\RTase.HR} NA P{i1-2Δ} NA P{I.954} NA P{I(neoRTW+)} NA P{I-element.fs02.T:HA1} NA P{I-element.S} NA P{I-element.T:HA1.Deltanuc} NA P{I-element.T:HA1.ΔA} NA P{I-element.T:HA1.ΔB} NA P{I-element.T:HA1.ΔCl3} NA P{I-element.T:HA1.ΔCl18} NA P{I-element.T:HA1.ΔCl21} NA P{I-element.T:HA1.ΔD} NA P{I-element.T:HA1.ΔE} NA P{I-element.T:HA1.ΔHind} NA P{I-element.T:HA1.ΔHp-Sn} NA P{I-element.T:HA1.Δnuc.Xba} NA P{I-element.T:HA1.ΔNuc} NA P{I-element.T:HA1} NA P{I-element\RTasei2Δ.hs} NA P{I-element\RTasei2Δmut.hs} NA P{IviP2} NA P{IyC1} NA P{W8-IΔZ} NA P{πI} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
ribonuclease H activity inferred from electronic annotation with InterPro:IPR002156 (FlyBase Curators et al., 2004-) RNA binding inferred from electronic annotation with InterPro:IPR000477 (FlyBase Curators et al., 2004-) RNA-directed DNA polymerase activity inferred from electronic annotation with InterPro:IPR000477 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
RNA-dependent DNA replication inferred from electronic annotation with InterPro:IPR000477 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	transposable_element_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions I-element\RTase allele Gene References
External Data
Linkouts
Orthologs
OrthoDB Orthologs (0) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search No orthologs identified Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search No orthologs identified Arthropod inclusive ortholog search No orthologs identified Metazoa inclusive ortholog search No orthologs identified
Orthologs in Drosophila Species (None identified)
No orthologies identified
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (None identified)
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (None identified)
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (None identified)
No non-Arthropod Metazoa orthologies identified
Human Orthologs (0)
Gene OMIM HGNC
AAA Orthologs (0) based on analysis using Dmel annotation version 4.3
No orthologs identified
Stocks & Reagents
Stocks Listed in FlyBase ( 0 )
Genomic Clones ( 0 )
cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Encoded by	I-element
Additional comments
Other Comments
	Sequence comparison of I-element\RTase constructs reveals that a 552bp segment, located in a central region of I-element\RTase is absent in all mutants exhibiting a Loc- phenotype. (Del Carmen Seleme et al., 2005) In vitro and in vivo analysis of I-element\RTase translation reveals that a bicistronic RNA homologous to the I-element enables I-element\RTase (ORF2) translation in vivo, strengthening the idea that the full length I-element RNA is not only the intermediate for transposition but also the messenger required for synthesis of the polypeptides catalysing this process. (Bouhidel et al., 1994) Ecol\CAT reporter gene constructs expressed in Drosophila culture cells and transformed flies identify a promoter that lies within the first 186bp of the I-element. (McLean et al., 1993)
External Crossreferences & Linkouts
Sequence Crossreferences
	DDBJ / EMBL / GenBank • AAA70222 M14954 UniProtKB/TrEMBL • Q24363
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Reverse transcriptase (IPR000477) Endonuclease/exonuclease/phosphatase (IPR005135) Ribonuclease H-like domain (IPR012337) Ribonuclease H domain (IPR002156)
Linkouts
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	I\T   I-element\RTase   I-Factor ORF2 (Van De Bor et al., 2005) ORF2 (Malinsky et al., 2000, Chaboissier et al., 2000, Jensen et al., 1999, Del Carmen Seleme et al., 1999, de la Roche Saint Andre and Bregliano, 1998, Malinsky et al., 1998, Busseau et al., 1998, Pritchard et al., 1988) ORF2p (Del Carmen Seleme et al., 2005)
Name Synonym	I-element ORF2 (Brennecke et al., 2008) I element reverse transcriptase   I-element transposase
Secondary FlyBase IDs
	FBgn0013982
References ( 33 )
Generate a list of	All references relating to this gene ( 33 )
List References by type	Research paper  ( 30 ) Personal communication to FlyBase  ( 1 ) Abstract  ( 2 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2011 Show research papers ...