• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\GluRIIB

General Information
Symbol	Dmel\GluRIIB	Species	D. melanogaster
Name	Glutamate receptor IIB	Annotation symbol	CG7234
Feature type	protein_coding_gene	FlyBase ID	FBgn0020429
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	dglurIIB
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	25E6-25E6	Sequence location	2L:5,559,342..5,563,502 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Glutamate receptor IIB is referred to in FlyBase by the symbol Dmel\GluRIIB (CG7234, FBgn0020429). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: glutamate receptor activity; ionotropic glutamate receptor activity. There is experimental evidence that it is involved in the biological process: positive regulation of synaptic transmission, glutamatergic; positive regulation of neuromuscular synaptic transmission. 13 alleles are reported. The phenotype of these alleles is annotated with: neuromuscular junction. It has one annotated transcript and one annotated polypeptide. Protein features are: Extracellular ligand-binding receptor; Extracellular solute-binding protein, family 3; Glutamate receptor, L-glutamate/glycine-binding; Ionotropic glutamate receptor; NMDA receptor. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed within 12-18 embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1625654_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of GluRIIB. Gene sequence location is 2L:5559342..5563502.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02	Controlled Vocabulary Terms positive regulation of neuromuscular synaptic transmission regulation of synaptic activity positive regulation of synaptic transmission, glutamatergic ionotropic glutamate receptor complex ionotropic glutamate receptor activity
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 25E6-25E6   Limits computationally determined from genome sequence between P{lacW}Hel25Ek11511 and P{lacW}l(2)k06502k06502
Experimentally Determined Cytological Location
Cytogenetic map Notes References 25F-25F     (Petersen, 1998.1.20) 25F-25F   (determined by in situ hybridisation)   (Petersen et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\GluRIIB for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	DGC clone SD17808 appears problematic: incomplete CDS
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) GluRIIB-RI FBtr0089577  NM_078761   2949   913
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein GluRIIB-PI   FBpp0088541   104.3   913   6.38     NP_523485   AAF52269
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments	Antibody raised to C-terminal peptide -ASSAKKKKKTRRIEK (Marrus et al., 2004)
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Ionotropic glutamate receptor (IPR001320) NMDA receptor (IPR001508) Extracellular ligand-binding receptor (IPR001828) Extracellular solute-binding protein, family 3 (IPR001638) Glutamate receptor, L-glutamate/glycine-binding (IPR019594)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE014134 AAF52269   AF044202 AAC00192   BI636198   CS133221   EY198608   FE527367   FE527370   FE527552   FE527620   FL502850
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VMP3 O45028
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag Stage Tissue/Position (including subcellular localization) Reference embryonic stage 17   embryonic/larval neuromuscular junction   (Saitoe et al., 2001) immunolocalization Stage Tissue/Position (including subcellular localization) Reference third instar larval stage   somatic muscle   • neuromuscular junction (Marrus et al., 2004) somatic muscle   • type I terminal bouton (Marrus et al., 2004) somatic muscle   • type Is terminal bouton (Marrus et al., 2004) somatic muscle   • type Ib terminal bouton (Marrus et al., 2004)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
ionotropic glutamate receptor complex inferred from physical interaction with GluRIIC, GluRIID, GluRIIA, GluRIIE (Qin et al., 2005) neuromuscular junction inferred from direct assay (Karr et al., 2009, Kohsaka et al., 2007, Petersen et al., 1997, Qin et al., 2005) colocalizes_with postsynaptic membrane inferred from direct assay (Kohsaka et al., 2007) postsynaptic membrane inferred from direct assay (Qin et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Little or no expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    12.175 Larval Midgut    14.4 Larval Hindgut    25.5 Larval Malpighian Tubules    14.4 Larval Fat Body    17.2 Larval Salivary Gland    11.1 Larval Trachea    6.925 Larval Carcass    73.4 Adult Head    9.9 Adult Eye    4.9 Adult Brain    9.8 Adult Thoracic-Abdominal Ganglion    6.8 Adult Crop    17.9 Adult Midgut    9.5 Adult Hindgut    22.9 Adult Malpighian Tubules    21.8 Adult Fat Body    22.2 Adult Salivary Gland    27.5 Adult Heart    16.225 Adult VirginFemale Spermatheca    23.8 Adult InseminatedFemale Spermatheca    29.1 Adult Ovary    5.6 Adult Testis    6.5 Adult Male Accessory Gland    17.5 Adult Carcass    19.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    9.9   NA  Eye    4.9   NA  Brain    9.8   12.175  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    6.8   NA  Crop    17.9   no informative data  Midgut    9.5   25.5  Hindgut    22.9   14.4  Malpighian Tubules    no informative data   no informative data  Fat Body    22.2   no informative data  Salivary Gland    no informative data   NA  Heart    16.225   6.925  Trachea    NA   NA  VirginFemale Spermatheca    23.8   NA  InseminatedFemale Spermatheca    no informative data   NA  Ovary    5.6   NA  Testis    6.5   NA  Male Accessory Gland    no informative data   73.4  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    0 central nervous system, larvae L3    0 central nervous system, pupae P8    0 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    0 head, mated 4-day male    0 head, mated 20-day male    0 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    0 digestive system, 1-day adult    0 digestive system, 4-day adult    0 digestive system, 20-day adult    0 fat body, larvae L3 wandering    0 fat body, white prepupae    0 fat body, pupae P8    0 carcass, larvae L3 wandering    2 carcass, 1-day adult    0 carcass, 4-day adult    0 carcass, 20-day adult    0 ovary, virgin 4-day female    0 ovary, mated 4-day female    0 testis, mated 4-day male    0 accessory gland, mated 4-day male    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderate expression to a trough of no expression detected. Peak expression observed within 12-18 embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    4 embryo 12-14hr    7 embryo 14-16hr    4 embryo 16-18hr    4 embryo 18-20hr    3 embryo 20-22hr    4 embryo 22-24hr    4 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    2 larva L3 puffstage 3-6    3 larva L3 puffstage 7-9    2 white prepupae new    1 white prepupae 12hr    0 white prepupae 24hr    1 pupae 2d postWPP    0 pupae 3d postWPP    0 pupae 4d postWPP    0 adult male 01day    0 adult male 05day    0 adult male 30day    0 adult female 01day    0 adult female 05day    0 adult female 30day    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    0 cold shock, 4-day adult    0 heat shock, 4-day adult    0 Cadmium 50 mM 6 hrs, larvae L3    1 Cadmium 50 mM 12 hrs, larvae L3    1 Cadmium 50 mM 48 hrs, 4-day adult    1 Cadmium 100 mM 48 hrs, 4-day adult    0 Copper 0.5 mM 12 hrs, larvae L3    0 Copper 15 mM 48 hrs, 4-day adult    0 Zinc 5 mM 12 hrs, larvae L3    1 Zinc 4.5 mM 48 hrs, 4-day adult    0 Ethanol 2.5% 3 hrs, larvae L3    0 Ethanol 5% 3 hrs, larvae L3    1 Ethanol 10% 3 hrs, larvae L3    0 Caffeine 1.5 mg/ml 4 hrs, larvae L3    1 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    0 Caffeine 25 mg/ml 48 hrs, 4-day adult    0 Paraquat 5 mM 48 hrs, 4-day adult    0 Paraquat 10 mM 48 hrs, 4-day adult    0 Rotenone 2 μg 12 hrs, larvae L3    1 Rotenone 8 μg 12 hrs, larvae L3    0 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_29 mE1_20_mRNA_expression_cluster_13
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020429 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7234-RI
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Dcr-2Scer\UAS.cDa, Scer\GAL4e22c, Scer\GAL4sqh.PW GluRIIBGD917 GluRIIBKK105825 viable, with Scer\GAL4pnr-MD237 GluRIIBGD917 Other Phenotypes Allele neuroanatomy defective, with Scer\GAL4Mhc.PW GluRIIBScer\UAS.cDa neurophysiology defective GluRIIBunspecified neurophysiology defective | larval stage GluRIIBMhc.T:Hsap\MYC wild-type GluRIIB+tg GluRIIBMhc.PD Phenotype manifest in Allele neuromuscular junction GluRIIBMhc.PD neuromuscular junction, with Scer\GAL4how-24B GluRIIBScer\UAS.cDa neuromuscular junction, with Scer\GAL4Mhc.PW GluRIIBScer\UAS.cDa neuromuscular junction | larval stage GluRIIBMhc.T:Hsap\MYC neuromuscular junction | larval stage, with Scer\GAL4Mhc.PW GluRIIBScer\UAS.cDa
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of GluRIIB Class Mutagen Stocks Known lesion GluRIIBMI03631 1 -- GluRIIBAD1 P-element activity 0 Yes GluRIIBunspecified 0 --
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of GluRIIB Class Mutagen Stocks Known lesion GluRIIBGD917 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes GluRIIBJF03145 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes GluRIIBKK105825 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes GluRIIB+t.cSa in vitro construct - genomic fragment 0 Yes GluRIIB+tg in vitro construct - genomic fragment 0 Yes GluRIIBGluRIIA.CTD.T:Avic\GFP-EGFP in vitro construct - genomic fragment in vitro construct - coding region fusion 0 Yes GluRIIBMhc.PD in vitro construct - regulatory fusion 0 Yes GluRIIBMhc.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes GluRIIBScer\UAS.cDa in vitro construct - regulatory fusion 0 Yes GluRIIBT:Avic\GFP-EGFP in vitro construct - coding region fusion in vitro construct - genomic fragment 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)cl-h4 (Sigrist et al., 2002) Df(2L)Exel6256 (Ryder, 2004.4.26) Df(2L)Exel7023 (Ryder, 2004.4.26) Df(2L)Exel8016 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD917} NA P{KK105825} NA P{TRiP.JF03145} NA P{UAS-GluRIIB.D} NA characterization construct P{GluRIIB.+t} NA P{GluRIIB.CTDGluRIIA-GFP} NA P{GluRIIB.GFP} NA P{GluRIIB+g} NA P{Mhc.GluRIIB.D} NA P{Mhc.GluRIIB.Myc} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions Mi{MIC}GluRIIBMI03631a NA Mi{MIC}GluRIIBMI03631b NA
Gene Ontology: Function, Process & Cellular Component ( 13 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
glutamate receptor activity inferred from direct assay (DiAntonio et al., 1997) ionotropic glutamate receptor activity inferred from mutant phenotype (Qin et al., 2005)
Biological Process
CV term Evidence References
positive regulation of neuromuscular synaptic transmission inferred from mutant phenotype (Qin et al., 2005) positive regulation of synaptic transmission, glutamatergic inferred from mutant phenotype (Qin et al., 2005)
Cellular Component
CV term Evidence References
ionotropic glutamate receptor complex inferred from physical interaction with GluRIIC, GluRIID, GluRIIA, GluRIIE (Qin et al., 2005) neuromuscular junction inferred from direct assay (Karr et al., 2009, Kohsaka et al., 2007, Petersen et al., 1997, Qin et al., 2005) colocalizes_with postsynaptic membrane inferred from direct assay (Kohsaka et al., 2007) postsynaptic membrane inferred from direct assay (Qin et al., 2005)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
extracellular-glutamate-gated ion channel activity inferred from electronic annotation with InterPro:IPR001320, InterPro:IPR001508, InterPro:IPR019594 (FlyBase Curators et al., 2004-) kainate selective glutamate receptor activity inferred from sequence or structural similarity (Littleton and Ganetzky, 2000) inferred from sequence or structural similarity with human GRIK1 (Curators, 2008--)
Biological Process
CV term Evidence References
ion transport inferred from electronic annotation with InterPro:IPR001508 (FlyBase Curators et al., 2004-) regulation of synaptic activity inferred from sequence or structural similarity with GluRIIA (Curators, 2008--)
Cellular Component
CV term Evidence References
outer membrane-bounded periplasmic space inferred from electronic annotation with InterPro:IPR001638 (FlyBase Curators et al., 2004-) plasma membrane inferred from sequence or structural similarity with GluRIIA (Petersen et al., 1997)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
GluRIIB - Neto anti tag coimmunoprecipitation, western blot (Kim et al., 2012)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions GluRIIB allele Gene References GluRIIBScer\UAS.cDa GluRIIA (Sigrist et al., 2002) unspecified GluRIIA (DiAntonio et al., 1997)
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0020429
Orthologs
OrthoDB Orthologs (65) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6NKCDX Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6QVB71 Arthropod inclusive ortholog search EOG6KH193 Metazoa inclusive ortholog search EOG6B61ZS
Orthologs in Drosophila Species (EOG6NKCDX)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\GluRIIB     Drosophila simulans   Dsim\GD23353     Drosophila sechellia   Dsec\GM18562     Drosophila erecta   Dere\GG25085     Drosophila yakuba   Dyak\GE25526     Drosophila ananassae   Dana\GF15573     Drosophila pseudoobscura pseudoobscura   Dpse\GA20200     Drosophila persimilis   Dper\GL26369     Drosophila willistoni   Dwil\GK14684 Y   Drosophila virilis   Dvir\GJ15540 Y   Drosophila mojavensis   Dmoj\GI17554 Y   Drosophila grimshawi   Dgri\GH11035 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6QVB71)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\LOC551704 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG009120 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG021904 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH22303 Y Atta cephalotes Leafcutter ant  Acep\ACEP24383 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO14545 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18405 Y Linepithema humile Argentine ant  Lhum\LH15532 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB25492 Y Solenopsis invicta Red fire ant  Sinv\SINV14316 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI001423 Y Bombyx mori Silkmoth  Bmor\BGIBMGA001026 Y Pediculus humanus Human body louse  Phum\PHUM055380 Y Tribolium castaneum Red flour beetle  Tcas\GLEAN_07128 Y
Orthologs in non-Insect Arthropods (EOG6KH193)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\MT-CYB Y
Orthologs in non-Arthropod Metazoa (EOG6B61ZS)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\glr-5 Y Caenorhabditis elegans Nematode  Cele\glr-3 Y Caenorhabditis elegans Nematode  Cele\glr-4 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_015492gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_014664gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028455gn Y Danio rerio Zebrafish  Drer\ENSDARG00000077715 Y Danio rerio Zebrafish  Drer\ENSDARG00000040627 Y Danio rerio Zebrafish  Drer\ENSDARG00000074515 Y Danio rerio Zebrafish  Drer\si:ch211-264f5.8 Y Danio rerio Zebrafish  Drer\grik5 Y Danio rerio Zebrafish  Drer\ENSDARG00000091173 Y Danio rerio Zebrafish  Drer\grik4 Y Danio rerio Zebrafish  Drer\grik1a Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000013277 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000014704 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000031503 Y Xenopus tropicalis Western clawed frog  Xtro\grik1 Y Xenopus tropicalis Western clawed frog  Xtro\grik4 Y Gallus gallus Domestic chicken  Ggal\GRIK1 Y Gallus gallus Domestic chicken  Ggal\GRIK4 Y Gallus gallus Domestic chicken  Ggal\GRIK3 Y Gallus gallus Domestic chicken  Ggal\GRIK2 Y Mus musculus House mouse  Mmus\Grik2 Y Mus musculus House mouse  Mmus\Grik3 Y Mus musculus House mouse  Mmus\Grik5 Y Mus musculus House mouse  Mmus\Grik1 Y Mus musculus House mouse  Mmus\Grik4 Y Rattus norvegicus Norway rat  Rnor\Grik3 Y Rattus norvegicus Norway rat  Rnor\Grik4 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000000368 Y Rattus norvegicus Norway rat  Rnor\Grik5 Y Rattus norvegicus Norway rat  Rnor\Grik1 Y Homo sapiens Human  Hsap\GRIK1 Y Homo sapiens Human  Hsap\GRIK2 Y Homo sapiens Human  Hsap\GRIK4 Y Homo sapiens Human  Hsap\GRIK3 Y Homo sapiens Human  Hsap\GRIK5 Y
Human Orthologs (5)
Gene OMIM HGNC Hsap\GRIK1     Hsap\GRIK2     Hsap\GRIK4     Hsap\GRIK3     Hsap\GRIK5
AAA Orthologs (4) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila willistoni Dwil\GK14684 Drosophila virilis Dvir\GJ15540 Drosophila mojavensis Dmoj\GI17554 Drosophila grimshawi Dgri\GH11035
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	28718 y1 v1; P{TRiP.JF03145}attP2 37066 y1 w*; Mi{MIC}GluRIIBMI03631a Mi{MIC}GluRIIBMI03631b
VDRC	v105581 P{KK105825}VIE-260B v7878 w1118; P{GD917}v7878
Genomic Clones ( 1 )
	BACR35L07 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 3 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones	DMG3-chr2L.639.pred.a DMG4-chr2L.10.010.pred.a SD17808
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0020429 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33789
Antibody Information
	polyclonal (Marrus et al., 2004)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: GluRIIB CG7234 (Skupski, 1999.11)
Source for database merge of
Additional comments
Other Comments
	GluRIIC is required for synaptic localisation of GluRIIA and GluRIIB proteins. Either GluRIIA or GluRIIB, but not both, is required for synaptic localisation of GluRIIC protein. GluRIIA and GluRIIB proteins compete with each other for access to GluRIIC protein and subsequent localisation to the synapse. (Marrus et al., 2004) Animals doubly mutant for GluRIIA and GluRIIB show late embryonic lethality, which can be rescued by transgenes expressing either gene alone. (DiAntonio et al., 1997) Glutamate receptor channels in Drosophila embryos and larvae have been studied using the patch-clamp technique. (Nishikawa and Kidokoro, 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078761 RefSeq (Proteins) • NP_523485 DDBJ / EMBL / GenBank • AAC00192 AAF52269 AF044202 BI636198 CS133221 Entrez Gene - A searchable database of RefSeq genes. • 33789 UniProtKB/TrEMBL • O45028 Q9VMP3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Ionotropic glutamate receptor (IPR001320) NMDA receptor (IPR001508) Extracellular ligand-binding receptor (IPR001828) Extracellular solute-binding protein, family 3 (IPR001638) Glutamate receptor, L-glutamate/glycine-binding (IPR019594)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0020429 DRSC - Results from RNAi screens. • FBgn0020429 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020429 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG7234-RI FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0020429 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33789 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /hjmuller/glutrc1.htm modMine - Data generated by the modENCODE project. • FBgn0020429
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG7234 (Benton et al., 2009, Croset et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Tsubouchi et al., 2012) DGluIIB (DiAntonio et al., 1997) dglurIIB (Sigrist et al., 2002) dGluRIIB (Ma et al., 2001) DGluRIIB (Griffith, 2004, Marrus et al., 2004, Saitoe et al., 2001, Petersen, 1998.1.20, DiAntonio et al., 1999, Davis and Goodman, 1998, Davis et al., 1998, Petersen et al., 1997, Rivlin et al., 2004, van der Plas et al., 2006, Guerrero et al., 2005, Wairkar et al., 2008, Lee et al., 2008, Owald et al., 2010) DGluR-IIB (Saitoe et al., 2001) DmelGluRIIB (Croset et al., 2010) glurIIB (Featherstone et al., 2001) GluRIIb (Krishnan et al., 2001) Glu-RIIB   GluRIIB (Augustin et al., 2007, Cheng et al., 2011, Haas et al., 2007, Tsai et al., 2012, Chen et al., 2005, Rasse et al., 2005, Weyhersmüller et al., 2011, Chen et al., 2009, Lee et al., 2009, Nahm et al., 2010, Schmid et al., 2006, Kohsaka et al., 2007, Pan and Broadie, 2007, Benton et al., 2009, Chen and Featherstone, 2005, Giagtzoglou et al., 2009, Schmid et al., 2008, Karr et al., 2009, Ramachandran et al., 2009, Chai et al., 2008, Chen et al., 2010, Higashi-Kovtun et al., 2010, Wang et al., 2010, Croset et al., 2010, Morimoto et al., 2010, Pilgram et al., 2011, Sun et al., 2011, Jordán-Álvarez et al., 2012, Wang et al., 2011, Chen and Ganetzky, 2012, Owald et al., 2012, Tsubouchi et al., 2012, Ganesan et al., 2011, Kim et al., 2012) unnamed (Landmesser, 1998, Nishikawa and Kidokoro, 1995)
Name Synonym	glutamate receptor (Verstreken and Bellen, 2002) Glutamate receptor IIB (Tsubouchi et al., 2012)
Secondary FlyBase IDs
References ( 83 )
Generate a list of	All references relating to this gene ( 83 )
List References by type	Research paper  ( 57 ) Supplementary material  ( 6 ) Review  ( 5 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 3 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 11 )
	Chen and Ganetzky, 2012, J. Cell Biol. 196(4): 529--543 A neuropeptide signaling pathway regulates synaptic growth in Drosophila. [FBrf0217500] Jordán-Álvarez et al., 2012, J. Cell Sci. 125(15): 3621--3629 Presynaptic PI3K activity triggers the formation of glutamate receptors at neuromuscular terminals of Drosophila. [FBrf0219610] Kim et al., 2012, Genes Dev. 26(9): 974--987 Drosophila Neto is essential for clustering glutamate receptors at the neuromuscular junction. [FBrf0218161] Owald et al., 2012, Nat. Neurosci. 15(9): 1219--1226 Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly. [FBrf0219330] Tsai et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(43): 17699--17704 Activity-dependent retrograde laminin A signaling regulates synapse growth at Drosophila neuromuscular junctions. [FBrf0219768] Cheng et al., 2011, J. Cell Biol. 194(6): 921--935 S6 kinase localizes to the presynaptic active zone and functions with PDK1 to control synapse development. [FBrf0216911] Ganesan et al., 2011, RNA Biol. 8(5): 771--781 Drosophila glutamate receptor mRNA expression and mRNP particles. [FBrf0217952] Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Sun et al., 2011, J. Neurosci. 31(2): 687--699 Neuroligin 2 is required for synapse development and function at the Drosophila neuromuscular junction. [FBrf0212770] Wang et al., 2011, J. Neurosci. 31(8): 2868--2877 Protein misfolding and oxidative stress promote glial-mediated neurodegeneration in an alexander disease model. [FBrf0213281] Weyhersmüller et al., 2011, J. Neurosci. 31(16): 6041--6052 Rapid Active Zone Remodeling during Synaptic Plasticity. [FBrf0213525] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...