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General Information
Symbol
Dmel\GluRIIB
Species
D. melanogaster
Name
Glutamate receptor IIB
Annotation Symbol
CG7234
Feature Type
FlyBase ID
FBgn0020429
Gene Model Status
Stock Availability
Gene Snapshot
Glutamate receptor IIB (GluRIIB) encodes a subunit of the muscle glutamate receptor that responds to neurotransmitter at the neuromuscular junction. [Date last reviewed: 2019-09-12]
Also Known As

DGluRIIB

Key Links
Genomic Location
Cytogenetic map
Sequence location
2L:5,559,342..5,563,502 [+]
Recombination map

2-17

RefSeq locus
NT_033779 REGION:5559342..5563502
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
NON-NMDA IONOTROPIC GLUTAMATE RECEPTORS - GLURII NEUROMUSCULAR JUNCTION-SUBTYPE -
In Drosophila, neuromuscular junction type A and type B glutamate receptors are heterotetramers assembled from either GluRIIA or GluRIIB plus other GluRII subunits. In response to the neurotransmitter, glutamate, they conduct Ca2+. (Adapted from FBrf0228432).
Summary (Interactive Fly)

transmembrane Ca2+ channel protein - expressed on muscular synaptic boutons targeted by motor neurons

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\GluRIIB or the JBrowse view of Dmel\GluRIIB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0089577
2949
913
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0088541
104.3
913
6.38
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments

Antibody raised to C-terminal peptide -ASSAKKKKKTRRIEK

External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\GluRIIB using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (18 terms)
Molecular Function (7 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001826301
(assigned by GO_Central )
inferred from sequence or structural similarity
inferred from sequence or structural similarity with HGNC:4579
inferred from biological aspect of ancestor with PANTHER:PTN000437926
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN000437926
(assigned by GO_Central )
Biological Process (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from direct assay
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001826301
(assigned by GO_Central )
inferred from sequence or structural similarity with FLYBASE:GluRIIA; FB:FBgn0004620
inferred from biological aspect of ancestor with PANTHER:PTN001826301
(assigned by GO_Central )
Cellular Component (6 terms)
Terms Based on Experimental Evidence (4 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
inferred from sequence or structural similarity with FLYBASE:GluRIIA; FB:FBgn0004620
inferred from biological aspect of ancestor with PANTHER:PTN000437926
(assigned by GO_Central )
inferred from biological aspect of ancestor with PANTHER:PTN001826301
(assigned by GO_Central )
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

GluRIIB transcripts are expressed exclusively in muscle. They are first detected in late stage 12 and reach maximal levels in stage 14 of embryogenesis. They are still present in the somatic musculature in stages 15-17 but at lower levels. GluRIIB transcripts are also detected at low levels in gut-associated muscle.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
epitope tag
Stage
Tissue/Position (including subcellular localization)
Reference
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\GluRIIB in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of GluRIIB
Transgenic constructs containing regulatory region of GluRIIB
Deletions and Duplications ( 4 )
Phenotypes
Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
No
3 of 15
Yes
No
3 of 15
Yes
No
2 of 15
No
No
2 of 15
No
No
 
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (8)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
3 of 15
Yes
No
3 of 15
Yes
No
3 of 15
Yes
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (7)
3 of 13
Yes
No
3 of 13
Yes
No
3 of 13
Yes
No
2 of 13
No
No
2 of 13
No
No
1 of 13
No
Yes
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (5)
2 of 12
Yes
No
1 of 12
No
No
1 of 12
No
Yes
1 of 12
No
Yes
1 of 12
No
No
Danio rerio (Zebrafish) (13)
3 of 15
Yes
No
3 of 15
Yes
No
3 of 15
Yes
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (5)
2 of 15
Yes
No
2 of 15
Yes
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (20)
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
3 of 9
Yes
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091901Y3 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501NY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Lucilia cuprina
Australian sheep blowfly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G02LN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (24)
5 of 10
5 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
3 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 1 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Show neighbor-neighbor interactions:
    Select Layout:
    Legend:
    Protein
    RNA
    Selected Interactor(s)
    Interactions Browser

    Please see the Physical Interaction reports below for full details
    protein-protein
    Physical Interaction
    Assay
    References
    RNA-RNA
    Physical Interaction
    Assay
    References
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement

    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-17

    Cytogenetic map
    Sequence location
    2L:5,559,342..5,563,502 [+]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    25E6-25E6
    Limits computationally determined from genome sequence between P{lacW}Hel25Ek11511 and P{lacW}l(2)k06502k06502
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    25F-25F
    (determined by in situ hybridisation)
    Experimentally Determined Recombination Data
    Location
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (9)
    Genomic Clones (11)
     

    Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

    cDNA Clones (2)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
      Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
          Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            Antibody Information
            Laboratory Generated Antibodies
            Commercially Available Antibodies
             
            Other Information
            Relationship to Other Genes
            Source for database identify of

            Source for identity of: GluRIIB CG7234

            Source for database merge of
            Additional comments
            Other Comments

            GluRIIC is required for synaptic localisation of GluRIIA and GluRIIB proteins. Either GluRIIA or GluRIIB, but not both, is required for synaptic localisation of GluRIIC protein. GluRIIA and GluRIIB proteins compete with each other for access to GluRIIC protein and subsequent localisation to the synapse.

            Animals doubly mutant for GluRIIA and GluRIIB show late embryonic lethality, which can be rescued by transgenes expressing either gene alone.

            Glutamate receptor channels in Drosophila embryos and larvae have been studied using the patch-clamp technique.

            Origin and Etymology
            Discoverer
            Etymology
            Identification
            External Crossreferences and Linkouts ( 33 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
            KEGG Genes - Molecular building blocks of life in the genomic space.
            modMine - A data warehouse for the modENCODE project
            Linkouts
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            FlyMine - An integrated database for Drosophila genomics
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Synonyms and Secondary IDs (14)
            Reported As
            Symbol Synonym
            DmelGluRIIB
            Glu-RIIB
            GluRIIB
            (Lecompte et al., 2020, Goel et al., 2019, Patrón et al., 2019, Tegeder et al., 2019, Akbergenova et al., 2018, Xing et al., 2018, Mallik et al., 2017, Perry et al., 2017, Van Vactor and Sigrist, 2017, Wang et al., 2017, Zhang et al., 2017, Zhang et al., 2017, Harris et al., 2016, Lee and Schwarz, 2016, Wang et al., 2016, Ziegler et al., 2016, Deivasigamani et al., 2015, Diao et al., 2015, Gene Disruption Project members, 2015-, Hu et al., 2015, Kim et al., 2015, Koles et al., 2015, Muhammad et al., 2015, Ramos et al., 2015, Gardiol and St Johnston, 2014, Ghosh et al., 2014, Machamer et al., 2014, Petzoldt et al., 2014, Sulkowski et al., 2014, Xing et al., 2014, Castellanos et al., 2013, Lee et al., 2013, Shi et al., 2013, Staples and Broadie, 2013, Chen and Ganetzky, 2012, Chorna and Hasan, 2012, Fukui et al., 2012, Jordán-Álvarez et al., 2012, Kim et al., 2012, Owald et al., 2012, Owald et al., 2012, Tsai et al., 2012, Tsubouchi et al., 2012, Cheng et al., 2011, Ganesan et al., 2011, Pilgram et al., 2011, Sun et al., 2011, Wang et al., 2011, Weyhersmüller et al., 2011, Chen et al., 2010, Croset et al., 2010, Higashi-Kovtun et al., 2010, Kong et al., 2010, Morimoto et al., 2010, Nahm et al., 2010, Wang et al., 2010, Benton et al., 2009, Chen et al., 2009, Giagtzoglou et al., 2009, Karr et al., 2009, Lee et al., 2009, Menon et al., 2009, Ramachandran et al., 2009, Chai et al., 2008, Schmid et al., 2008, Augustin et al., 2007, Haas et al., 2007, Kohsaka et al., 2007, Pan and Broadie, 2007, Schmid et al., 2006, Chen and Featherstone, 2005, Chen et al., 2005, Rasse et al., 2005)
            dGluRIIB
            Name Synonyms
            Glutamate receptor IIB
            glutamate receptor
            glutamate receptor IIB
            Secondary FlyBase IDs
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (142)