Fak56D, Fak56, DFAK, DFak56, pFAK
non-receptor tyrosine kinaseinvolved in integrin signaling - targets multiple cytoskeletal and signal transduction proteins - functions include embryonic muscle attachement, morphogensis of the optic stalk, synaptic growth and transmission, and in the regulation of RTK-MAP kinase signaling in epithelia
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.51
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Fak using the Feature Mapper tool.
Between 0 and 4 hours after puparium formation protein colocalizes with f-actin at the cell periphery with more prominent staining at the cells leading edge. Staining sometimes appears as large spots and small spots that increase in number during this period.
Fak protein is expressed ubiquitously during most of embryogenesis. From stage 13, levels are elevated in the gut and the CNS. Fak protein is abundant in germ cells and follicle cells at early stages of oogenesis but decreases by stage 6. The protein persists in follicle cells in the anterior part of the stage 6 egg chamber. At stage 9, Fak protein is prominant in the border cells during their migration.
Fak protein is detected by immunolocalization at muscle attachment sites. It is expressed predominantly in the epidermal cells and less or not at all in the muscle cells.
GBrowse - Visual display of RNA-Seq signalsView Dmel\Fak in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Fak Fak56D
FlyBase curator comment: Renamed from 'Fak56D' to 'Fak' because: (i) there is only one FAK ortholog in the genome, so there is no need for a distinguishing suffix; (ii) there was disagreement in the three original publications (FBrf0123155, FBrf0123015, FBrf0111365) about the cytological position (56A-B vs 56D) and, perhaps for this reason, they all refer to the gene as 'Fak56' (not 'Fak56D') anyway; (iii) 'Fak' is more widely used in the literature; (iv) reference to cytological position in a gene symbol/name is best avoided if possible.
Fak56D is required autonomously in the surface glial cells of the optic stalk for normal optic stalk morphogenesis during development.
Fak56D is not essential for integrin functions in vivo, however, overexpressed Fak56D is a potent inhibitor of integrins binding to the extracellular matrix, suggesting that Fak56D may play a subtle role in the negative regulation of integrin adhesion.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in S2R+ cells: cells become retracted (unspread but flat). Kc167 cells are unaffected.