dCtBP, G9, l(3)G9
Annotated transcripts do not represent all supported alternative splices within 5' UTR.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.47
Gene model reviewed during 6.19
4.0, 3.5, 2.7, 2.5 (northern blot)
CtBP protein interacts with h protein. The region of h protein required for the interaction was mapped to a 25aa region immediately upstream of the C-terminal WRPW motif. Deletion of 5 amino acids within this region (PLSLV) completely abolishes binding of h protein to CtBP protein. The region of the CtBP protein required for the interaction maps to the first 376 amino acids which are common to all four isoforms of CtBP protein that have been identified.
Homodimer. Interacts with hairy (h), knirps (kni), snail (sna), and Enhancer of split m-delta (HLHm-delta). Complex may be involved in transcriptional repression. Interacts also with adenovirus E1A protein.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CtBP using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\CtBP in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
DNA-protein interactions: genome-wide binding profile assayed for CtBP protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069).
Upon wg signalling, the CtBP product exhibits transcription coactivator activity, which is dependent upon at least the arm protein. In the absence of wg signalling, the CtBP product exhibits transcription repressor activity.
dsRNA made from templates generated with primers directed against this gene has been transfected into Kc cells.
dsRNA made from templates generated with primers directed against this gene used in a cell-based RNAi assay to identify components or modifiers of the JAK/STAT pathway.
CtBP contributes quantitatively, rather than qualitatively, to transcriptional repression.
Identification: Enhancer trap screen designed to discover genes involved in the cellular aspects of defense mechanisms, as well as in melanotic tumor formation processes linked to blood cell disregulation.