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Gene Dmel\Abi

General Information
Symbol	Dmel\Abi	Species	D. melanogaster
Name	Abelson Interacting Protein	Annotation symbol	CG9749
Feature type	protein_coding_gene	FlyBase ID	FBgn0020510
Created / Updated	2003-12-01/2007-03-20
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	88A9-88A9	Sequence location	3R:9,944,982..9,948,142 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Abelson Interacting Protein is referred to in FlyBase by the symbol Abi (CG9749, FBgn0020510). It has the cytological map location 88A9. Its sequence location is 3R:9944982..9948142. Its molecular function is described as: signal transducer activity; protein binding. It is involved in the biological processes: positive regulation of protein kinase activity; cell projection biogenesis; cortical actin cytoskeleton organization and biogenesis; regulation of cell shape; phagocytosis, engulfment; cell adhesion; muscle attachment; lamellipodium biogenesis. 7 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 88A9-88A9   Limits computationally determined from genome sequence between P{PZ}flfl01949 and P{lacW}trxj14A6&P{PZ}trx00347
Experimentally Determined Cytological Location
Cytogenetic map Notes References 88A-88B (determined by in situ hybridisation)   (Gangadharan and Tear, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References Overall orientation not stated: Abi- twf+ 140up- (Wahlstrom et al., 2001)
Gene Model & Products
Please see the GBrowse view of Dmel\Abi for information on other features
Comments on Gene Model
	EST data support existence of multiple transcripts due to variable use of 5' exons
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Abi-RA FBtr0082910  NM_057915   2381   473 Abi-RB FBtr0112896  NM_001104315   2375   473
Additional Transcript Data & Comments
Reported size (kB)	1.7 (longest cDNA) (Juang and Hoffmann, 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein Abi-PA   FBpp0082371   51.9   473   6.93     NP_477263   AAF55021 Abi-PB   FBpp0111809   51.9   473   6.93     NP_001097785   ABW08669
Additional Polypeptide Data & Comments
Reported size (kD)	473 (aa); 52 (kD observed) (Juang and Hoffmann, 1999)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0020510
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Neutrophil cytosol factor 2 (IPR000108) Src homology-3 (IPR001452) Abl-interactor, homeo-domain homologous region (IPR012849)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA949993   AA951542   AC007441   AC007904   AE003703 AAF55021   AF151115 AAD38382   AI456605   AI456858   AI457032   AI516875   AI516885   AI519260   AI531987   AI534023   AI534026   AI541715   AW943364   AY051877 AAK93301   BF506031   BG635570   BG636879   BI161716   BI161717   BI165171   BI212807   BI228726   BI228860   BI228874   BI236593   BI357029   BI361342   BI366630   BI368086   BI368437   BI372841   BI374470   BI483470   BI483788   BI485711   BI485937   BI486535   BI486663   BI577944   BI579310   BI580622   BI632194   BI638387   CK660561   CO155030   CO273172   CO279512   CO293212   CO296753   CO336187   EC075192   EC076432   EC083682   EC220126   EC235173   EC265053   EC267450   RE01144
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9Y0S9
Maps to	FBcl0298056 FBcl0298057 (FlyBase, 1992-)
Does NOT map to
Identified with	LD37010 (BDGP Project Members, 2000-)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 9381
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Microarray Data
	Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) AA949993 AI456858
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020510 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0020510 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0020510
Alleles & Phenotypes
Summary of Allele Phenotypes
Other Phenotypes Allele cell shape defective AbidsRNA.cKa Phenotype manifest in Allele
Classical Alleles ( 1 )
For All Classical Alleles Show
Allele of Abi Class Mutagen Stocks Known lesion AbiEY20423 P-element activity 1 --
Alleles Carried on Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Abi Class Mutagen Stocks Known lesion AbiAct5C.T:Ivir\HA1 in vitro construct | regulatory fusion in vitro construct | coding region fusion 0 Yes AbiGD14302 in vitro construct | RNAi in vitro construct | regulatory fusion 1 Yes Abia.Act5C in vitro construct | regulatory fusion 0 Yes AbidsRNA.cKa in vitro construct | RNAi 0 Yes AbidsRNA.cRa in vitro construct | RNAi 0 Yes AbiΔSH3.Act5C.T:Ivir\HA1 in vitro construct | deletion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(3R)red-P52
Disrupted in	Df(3R)Exel7359 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14302} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}AbiEY20423 NA
Related Comments
	Please look at the allele reports for the complete phenotype data S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006)
Gene Ontology: Function, Process & Cellular Component ( 13 )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with FLYBASE:Abl; FB:FBgn0000017 (Juang and Hoffmann, 1999) inferred from physical interaction with FLYBASE:Hem; FB:FBgn0011771 AND inferred from physical interaction with FLYBASE:WASp; FB:FBgn0024273 (Bogdan and Klambt, 2003) inferred from physical interaction with FLYBASE:Ptp61F; FB:FBgn0003138 (Huang et al., 2007)
signal transducer activity inferred from sequence or structural similarity with EMBL:U17698 (FlyBase, 1992-)
Biological Process
CV term Evidence References
cell adhesion inferred from mutant phenotype (Jani and Schock, 2007)
cell projection biogenesis inferred from mutant phenotype (Kunda et al., 2003)
cortical actin cytoskeleton organization and biogenesis inferred from mutant phenotype (Kunda et al., 2003)
lamellipodium biogenesis inferred from mutant phenotype (Huang et al., 2007)
muscle attachment inferred from mutant phenotype (Jani and Schock, 2007)
phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006)
positive regulation of protein kinase activity inferred from direct assay (Juang and Hoffmann, 1999)
regulation of cell shape inferred from mutant phenotype (Kunda et al., 2003)
Cellular Component
CV term Evidence References
cytoplasm inferred from electronic annotation with InterPro:IPR012849 (FlyBase Curators et al., 2004-)
lamellipodium membrane inferred from direct assay (Huang et al., 2007)
perinuclear region of cytoplasm inferred from direct assay (Huang et al., 2007)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Abi allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 66795
Orthologs
Genome-wide drosophilid orthologs	Dana\GF18788 Dere\GG21491 Dgri\GH19698 Dmoj\GI24554 Dper\GL23309 Dpse\GA22007 Dsec\GM25884 Dsim\GD20455 Dvir\GJ22620 Dwil\GK10907 Dyak\GE10059
Curated drosophilid orthologs	Dpse\GA22007
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000020087 Anopheles gambiae (Ensembl) ENSBTAP00000014633 Bos taurus (Ensembl) ENSCAFP00000006495 Canis familiaris (Ensembl) NEWSINFRUP00000140951 Takifugu rubripes (Ensembl) ENSGALP00000012187 Gallus gallus (Ensembl) ENSGALP00000014164 Gallus gallus (Ensembl) ENSP00000295851 Homo sapiens (Ensembl) ENSMMUP00000000419 Macaca mulatta (Ensembl) ENSMODP00000010567 Monodelphis domestica (Ensembl) ENSPTRP00000021934 Pan troglodytes (Ensembl) ENSRNOP00000025215 Rattus norvegicus (Ensembl) GSTENP00015061001 Tetraodon nigroviridis (Ensembl) ENSXETP00000008999 Xenopus tropicalis (Ensembl) Q5NB83 Oryza sativa (Gramene) WBGene00030670 Caenorhabditis briggsae (Wormbase) WBGene00015146 Caenorhabditis elegans (Wormbase) ZDB-CDNA-040425-2641 Danio rerio (Zfin) ZDB-GENE-040426-1701 Danio rerio (Zfin) DDB0231426 Dictyostelium discoideum (Dictybase) MGI:106913 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 2 )
VDRC	v36142 w1118; P{GD14302}v36142/TM3
Bloomington	23107 y1 w67c23; P{EPgy2}AbiEY20423
Genomic Clones ( 2 )
	BACR10E03 BACR01H04
cDNA Clones ( 47 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD37010
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	RE66372 RE53996 RE62068 RE26514 RE71721 RE60026 SD14693 SD13022 AT08382 RE52209 LD38782 RE74838 RE45519 GH27474 SD06754 LD32090 RE43368 RE26358 RE26531 RE32665 SD25837 SD06485 LD36681 RE05002 RE54407 RE69046 RE69977 SD03315 RE18046 LD28582 RE66733 SD06474 GH27462 LD37245 RE73324 SD20663 RE70118 LP19221 RE69301
Other clones	EK202151 EN05234 EN12021 EK134920 RE01144 EK109908 EK215105
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0020510 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0020510
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: Abi CG9749 (Zheng, 1999.11)
Source for database merge of
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation. (Rogers et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057915 NM_001104315 RefSeq (Proteins) • NP_477263 NP_001097785 DDBJ / EMBL / GenBank • AC007441 AC007904 AE003703 AAF55021 AF151115 AAD38382 AI456858 AW943364 AY051877 AAK93301 UniProtKB/TrEMBL • Q9Y0S9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Neutrophil cytosol factor 2 (IPR000108) Src homology-3 (IPR001452) Abl-interactor, homeo-domain homologous region (IPR012849)
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 66795 DEDB - Drosophila exon database: splicing graphs • 9381 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0020510 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0020510 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • FBgn0020510 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0020510 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0020510 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0020510 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000020087 Anopheles gambiae (Ensembl) ENSBTAP00000014633 Bos taurus (Ensembl) ENSCAFP00000006495 Canis familiaris (Ensembl) NEWSINFRUP00000140951 Takifugu rubripes (Ensembl) ENSGALP00000012187 Gallus gallus (Ensembl) ENSGALP00000014164 Gallus gallus (Ensembl) ENSP00000295851 Homo sapiens (Ensembl) ENSMMUP00000000419 Macaca mulatta (Ensembl) ENSMODP00000010567 Monodelphis domestica (Ensembl) ENSPTRP00000021934 Pan troglodytes (Ensembl) ENSRNOP00000025215 Rattus norvegicus (Ensembl) GSTENP00015061001 Tetraodon nigroviridis (Ensembl) ENSXETP00000008999 Xenopus tropicalis (Ensembl) Q5NB83 Oryza sativa (Gramene) WBGene00030670 Caenorhabditis briggsae (Wormbase)