DRx, E97, PPH17, bk50
homeodomain transcription factor - required for regulation of genes involved in brain morphogenesis - promotes cell growth, proliferation and survival of mushroom body neuroblasts in the brain
Please see the JBrowse view of Dmel\Rx for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Mutation in sequenced strain: frameshift (-1).
Gene model reviewed during 5.51
3.2 (longest cDNA)
There is only one protein coding transcript and one polypeptide associated with this gene
902, 772 (aa); 96 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Rx using the Feature Mapper tool.
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Comment: reported as procephalic ectoderm anlage in statu nascendi
Rx transcripts are first observed at embryonic stage 8 in two dorsolateral spots in the procephalic region. At the end of stage 8, additional staining is seen in the dorsal region that will give rise to the clypeolabrum. The head region expression becomes more pronounced by stage 9 and additional staining appears in the CNS. Later expression is observed in the antenno-maxillary complex. Staining at the medial edges of the two brain lobes, in the clypeolabrum, and in the antenno-maxillary complex persists until the end of embryogenesis.
Rx transcripts are detected by in situ hybridization during early embryogenesis in the eye disc primordia. They are also observed in a patch of cells in the anterior of the embryo corresponding to the clypeolabrum, in a region that gives rise to the epiphysis.
Comment: 24 hours after hatching
Comment: 96 hours after hatching
Comment: 96 hours after hatching
Rx is expressed in subsets of neuroblasts in stage 16 embryos. It is also observed in mushroom body neuroblasts. In type II neuroblasts, it is expressed in the PDM and DL clusters. It is also expressed in many type 1 neuroblasts.
During embryogenesis, Rx is expressed in a subset of Nrt positive brain cells, including 8-10 dpn positive neuroblasts, 4 of which are the mushroom body neuroblasts. This expression, and co-expression with dpn, persists throughout larval development where it also overlaps with Scer\GAL4insc-Mz1407 and Scer\GAL4NP1316. During the first larval instar stage, Rx positive mushroom body neuroblasts are seen within the cortex of the ey positive larval mushroom body. At the L3 stage, there are ~10 Rx and dpn co-expressing neuroblasts per hemisphere including the mushroom body neuroblasts, 2 of the dorso-lateral protocerebral DPLc lineage and ~2 that are part of the protocerebral CP or posterior deutocerebral CM lineages. Rx expression also overlaps with pros in a subset of mushroom body cells at this stage. Rx is also expressed in distally located, elav positive differentiated neurons of the type II DM neuroblast lineage, but not in neuroblasts, precursor cells or ganglion mother cells of this lineage. Finally, Rx is expressed in a subset of young Scer\GAL4insc-Mz1407 labelled Kenyon cells in adults but is not observed in mature Kenyon cells.
Rx is expressed in type I but not in type II neuroblasts.
In stage 12 embryos, Rx protein is detected in a cluster of neuroectodermal cells alongside the dorsal midline, posterior to the pars lateralis primordium.
Protein is detected in stage 13 embryos in a restricted pattern in the central nervous system with staining in the embryonic brain hemispheres near the midline. Protein is expressed in larval central nervous system in several cell clusters near the midline. Additional expression in larvae is observed in a subset of cells in the clypeo-labral disc. Expression is detected in dorsal and posterior regions of the adult brain.
GBrowse - Visual display of RNA-Seq signals
View Dmel\Rx in GBrowse 2
2-92
2-99.5
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: Rx E97 wom
Although extensive DNA sequence for "E97" has not been determined, no sequence overlapping that of the homeodomain of "PPH17" has been identified. Cross hybridization analysis suggests that "E97" and "PPH17" represent different homeodomain genes at 57B.
Rx is cloned and sequenced and the gene structure and expression pattern determined.
One of the homeodomain loci identified in a screen for genes encoding DNA binding proteins capable of binding to a consensus Engrailed binding site.
Identified on the basis of similarity to the eve homeobox sequence.
