orbit, CLASP, mast, multiple asters, v40
microtubule-associated protein that plays an essential role facilitating the kinetochore-microtubule interaction - maternially it facilitates multiple interactions of the fusome with mitotic spindles and ring canals - zygotically it mediates the action of Slit and its receptors acting as a partner of the Abelson tyrosine kinase
Gene model reviewed during 5.46
6.5, 6.0 (northern blot)
1492 (aa); 165 (kD observed)
Interacts with CLIP-190 and microtubules.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\chb using the Feature Mapper tool.
GBrowse - Visual display of RNA-Seq signalsView Dmel\chb in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: chb mast
Source for merge of: chb NEST:bs13a06 NEST:bs17d05 NEST:bs28g05
Source for merge of: chb BcDNA:LD31673
Source for merge of: chb CG32435
Source for merge of: chb anon-WO0104295
Source for merge of: chb MESR7 orbit
First exon of release 3.1 annotation CG32435-RB split into a distinct annotation, CG33486 (which corresponds to asparagine-synthetase), in release 3.2 of the genome annotation. Release 3.2 annotation CG32435 corresponds to chb.
Source for merge of chb MESR7 orbit was sequence comparison ( date:001216 ).
Source for merge of chb BcDNA:LD31673 was TrEMBL update ( date:020807 ).
Source for merge of chb CG32435 was sequence comparison ( date:021112 ).
Source for merge of chb anon-WO0104295 was sequence comparison ( date:030908 ).
S2 cells treated with RNAi against chb show reduced poleward flux rates of tubulin subunits in mitotic spindles and suppressed α-tubulin turnover at the microtubule plus ends of the spindles. There is a significantly increased scatter of chromosomes throughout the spindle in the RNAi treated cells compared to controls. Pole-to-pole spindle lengths are significantly reduced in the RNAi treated cells compared to controls.
RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.
S2 cells transfected with dsRNA made from templates generated with primers directed against this gene show shortening of the metaphase spindle.
chb is required for stem cell and cystocyte divisions during oogenesis. It is also required during mid-oogenesis for the organisation of the polarised microtubule network inside the 16-cell cyst that ensures oocyte differentiation.
New annotation (CG32435) in release 3 of the genome annotation.
Area matching Drosophila EST AA392551.
chb plays an essential role in centrosome separation and organisation of the bipolar mitotic spindle.
Considering the compact chromosome structure seen in the v40 mutant the authors propose the mutation is termed chromosome bows (chb).