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Gene Dmel\Nle

General Information
Symbol	Dmel\Nle	Species	D. melanogaster
Name	Notchless	Annotation symbol	CG2863
Feature type	protein_coding_gene	FlyBase ID	FBgn0021874
Gene Model Status	Current	Stock availability	5 publicly available
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	21E2-21E2	Sequence location	2L:566,366..568,121 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Notchless is referred to in FlyBase by the symbol Dmel\Nle (CG2863, FBgn0021874). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: Notch binding. Based on sequence similarity, it is predicted to be involved in the biological process: Notch signaling pathway. 8 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum; microchaeta. It has one annotated transcript and one annotated polypeptide. Protein features are: G-protein beta WD-40 repeat; G-protein, beta subunit; NLE; Quinonprotein alcohol dehydrogenase-like-superfamily; WD40 repeat; WD40 repeat, conserved site; WD40-repeat-containing domain; WD40/YVTN repeat-like-containing domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed during early larval stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult female reproductive system, adult male accessory gland. Comments on Affy2 ProbeSet: ProbeSet 1632721_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Nle. Gene sequence location is 2L:566366..568121.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 21E2-21E2   Limits computationally determined from genome sequence between P{lacW}l(2)k09610k09610&P{lacW}Sk09538a and P{PZ}l(2)1068510685&P{lacW}Tango14k00619
Experimentally Determined Cytological Location
Cytogenetic map Notes References 21C7-21C8   (determined by in situ hybridisation)   (Spradling et al., 1999) 21C7-21C8     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nle for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.45
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nle-RA FBtr0078075  NM_057946   1584   488
Additional Transcript Data & Comments
Reported size (kB)	1.521 (longest cDNA) (Royet et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nle-PA   FBpp0077735   53.8   488   6.93     NP_477294   AAF51479
Additional Polypeptide Data & Comments
Reported size (kDa)	480 (aa) (Royet et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • WD40/YVTN repeat-like-containing domain (IPR015943) WD40-repeat-containing domain (IPR017986) G-protein, beta subunit (IPR001632) WD40 repeat (IPR001680) NLE (IPR012972) WD40 repeat, conserved site (IPR019775) G-protein beta WD-40 repeat (IPR020472) Quinonprotein alcohol dehydrogenase-like superfamily (IPR011047)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141978   AA696993   AA735870   AE014134 AAF51479   AC004573   AC008002   AI518473   AI520108   AJ012588 CAA10070   AQ026066   AX025192   AY089286 AAL90024   BF492695   BF505998   BG633066   BI232464   BI232484   BI632742   BI635228   BI635777   BI637470   BI639652   BP553015   CK604865   CO276695   CO292085   CO298131   CO342939   CO343824   EC194372   EC199902   EC209057   EC224049   EC252499   EC259509   EC267179
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VPR4 O96995
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   anal pad primordium   (Fisher et al., 2012) Comment:reported as anal pad specific anlage anterior midgut primordium   (Fisher et al., 2012) dorsal pharyngeal muscle primordium   (Fisher et al., 2012) hindgut proper primordium   (Fisher et al., 2012) Malpighian tubule primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) proventriculus primordium   (Fisher et al., 2012) salivary gland body primordium   (Fisher et al., 2012) somatic muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium visceral muscle primordium   (Fisher et al., 2012) Comment:reported as muscle system primordium trunk mesoderm   (Fisher et al., 2012) embryonic stage 13 -- 16   dorsal pharyngeal muscle   (Fisher et al., 2012) embryonic anal pad   (Fisher et al., 2012) embryonic hindgut   (Fisher et al., 2012) embryonic Malpighian tubule   (Fisher et al., 2012) embryonic proventriculus   (Fisher et al., 2012) embryonic/larval lymph gland   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) embryonic/larval muscle system   (Fisher et al., 2012) embryonic/larval somatic muscle   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval trachea, adult female reproductive system, adult male accessory gland. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    143.525 Larval Midgut    42.3 Larval Hindgut    69.3 Larval Malpighian Tubules    38.6 Larval Fat Body    24.1 Larval Salivary Gland    95.6 Larval Trachea    119.725 Larval Carcass    68.3 Adult Head    55.2 Adult Eye    48.975 Adult Brain    61.2 Adult Thoracic-Abdominal Ganglion    75 Adult Crop    71.1 Adult Midgut    58.3 Adult Hindgut    58.9 Adult Malpighian Tubules    68.3 Adult Fat Body    63 Adult Salivary Gland    70.8 Adult Heart    49.775 Adult VirginFemale Spermatheca    77 Adult InseminatedFemale Spermatheca    112.8 Adult Ovary    119.3 Adult Testis    30.1 Adult Male Accessory Gland    103.3 Adult Carcass    56.4 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    55.2   NA  Eye    48.975   NA  Brain    61.2   143.525  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    75   NA  Crop    71.1   42.3  Midgut    58.3   69.3  Hindgut    58.9   38.6  Malpighian Tubules    68.3   24.1  Fat Body    63   95.6  Salivary Gland    70.8   NA  Heart    49.775   119.725  Trachea    NA   NA  VirginFemale Spermatheca    77   NA  InseminatedFemale Spermatheca    112.8   NA  Ovary    119.3   NA  Testis    30.1   NA  Male Accessory Gland    103.3   68.3  Carcass    56.4 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    9 central nervous system, larvae L3    7 central nervous system, pupae P8    3 head, virgin 1-day female    2 head, virgin 4-day female    4 head, virgin 20-day female    5 head, mated 1-day female    2 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    2 head, mated 4-day male    4 head, mated 20-day male    5 salivary gland, larvae L3 wandering    1 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    3 digestive system, 1-day adult    4 digestive system, 4-day adult    5 digestive system, 20-day adult    4 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    8 carcass, larvae L3 wandering    5 carcass, 1-day adult    2 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    8 ovary, mated 4-day female    7 testis, mated 4-day male    9 accessory gland, mated 4-day male    4 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed during early larval stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr    44 embryo 06-08hr    47 embryo 08-10hr    44 embryo 10-12hr    54 embryo 12-14hr    26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    61 larva L2    27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr  (44) embryo 06-08hr  (47) embryo 08-10hr  (44) embryo 10-12hr  (54) embryo 12-14hr  26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr  (29) embryo 22-24hr    24 larva L1  (61) larva L2  (27) larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr    44 embryo 06-08hr    47 embryo 08-10hr    44 embryo 10-12hr    54 embryo 12-14hr    26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    61 larva L2    27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr    44 embryo 06-08hr    47 embryo 08-10hr    44 embryo 10-12hr    54 embryo 12-14hr    26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    61 larva L2    27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr    44 embryo 06-08hr    47 embryo 08-10hr    44 embryo 10-12hr    54 embryo 12-14hr    26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    61 larva L2    27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr  (44) embryo 06-08hr  (47) embryo 08-10hr  (44) embryo 10-12hr  (54) embryo 12-14hr  26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr  29 embryo 22-24hr    24 larva L1  (61) larva L2  27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr    44 embryo 06-08hr    47 embryo 08-10hr    44 embryo 10-12hr    54 embryo 12-14hr    26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    61 larva L2    27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    10 embryo 02-04hr    17 embryo 04-06hr    44 embryo 06-08hr    47 embryo 08-10hr    44 embryo 10-12hr    54 embryo 12-14hr    26 embryo 14-16hr    21 embryo 16-18hr    15 embryo 18-20hr    14 embryo 20-22hr    29 embryo 22-24hr    24 larva L1    61 larva L2    27 larva L3 12hr old    16 larva L3 puffstage 1-2    8 larva L3 puffstage 3-6    9 larva L3 puffstage 7-9    10 white prepupae new    8 white prepupae 12hr    9 white prepupae 24hr    11 pupae 2d postWPP    8 pupae 3d postWPP    5 pupae 4d postWPP    7 adult male 01day    6 adult male 05day    7 adult male 30day    7 adult female 01day    24 adult female 05day    22 adult female 30day    23 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    41 Schneider line 2 Sg4    52 embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167    71 embryonic S1    18 embryonic S3    21 leg disc CME L1    27 wing disc CME-W2    27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    33 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    42 tumorous blood cells mbn2    26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (41) Schneider line 2 Sg4  (52) embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167  (71) embryonic S1    18 embryonic S3    21 leg disc CME L1  (27) wing disc CME-W2  (27) wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21  (33) wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1  (29) CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2  (42) tumorous blood cells mbn2  26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    41 Schneider line 2 Sg4    52 embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167    71 embryonic S1    18 embryonic S3    21 leg disc CME L1    27 wing disc CME-W2    27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    33 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    42 tumorous blood cells mbn2    26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    41 Schneider line 2 Sg4    52 embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167    71 embryonic S1    18 embryonic S3    21 leg disc CME L1    27 wing disc CME-W2    27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    33 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    42 tumorous blood cells mbn2    26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    41 Schneider line 2 Sg4    52 embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167    71 embryonic S1    18 embryonic S3    21 leg disc CME L1    27 wing disc CME-W2    27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    33 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    42 tumorous blood cells mbn2    26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (41) Schneider line 2 Sg4  (52) embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167  (71) embryonic S1    18 embryonic S3    21 leg disc CME L1  27 wing disc CME-W2  27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21  (33) wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5  (36) mixed discs ML-DmD4-c1  29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2  (42) tumorous blood cells mbn2  26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    41 Schneider line 2 Sg4    52 embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167    71 embryonic S1    18 embryonic S3    21 leg disc CME L1    27 wing disc CME-W2    27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    33 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    42 tumorous blood cells mbn2    26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    41 Schneider line 2 Sg4    52 embryonic 1182-4H    11 embryonic GM2    10 embryonic Kc167    71 embryonic S1    18 embryonic S3    21 leg disc CME L1    27 wing disc CME-W2    27 wing disc ML-DmD8    19 wing disc ML-DmD9    12 wing disc ML-DmD16-c3    16 wing disc ML-DmD21    33 wing disc ML-DmD32    18 haltere disc ML-DmD17-c3    25 eye-antennal disc ML-DmD11    17 antennal disc ML-DmD20-c5    36 mixed discs ML-DmD4-c1    29 CNS ML-DmBG1-c1    25 CNS ML-DmBG2-c2    42 tumorous blood cells mbn2    26 ovary fGS/OSS    11 ovary OSC    5 ovary OSS    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    3 cold shock, 4-day adult    1 heat shock, 4-day adult    8 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    4 Cadmium 50 mM 48 hrs, 4-day adult    9 Cadmium 100 mM 48 hrs, 4-day adult    6 Copper 0.5 mM 12 hrs, larvae L3    4 Copper 15 mM 48 hrs, 4-day adult    2 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    4 Ethanol 2.5% 3 hrs, larvae L3    9 Ethanol 5% 3 hrs, larvae L3    4 Ethanol 10% 3 hrs, larvae L3    7 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    5 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    10 Paraquat 10 mM 48 hrs, 4-day adult    13 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    11 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_08 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • AT08344 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0021874 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2863-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Nle Stages(s) 1-3 Stages(s) 4-6 Stages(s) 9-10 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4dpp.blk1 NleGD9836 lethal, with Scer\GAL4en-e16E NleGD9836 lethal | pupal stage, with Scer\GAL4pnr-MD237 NleGD9836 lethal | recessive Nlek13714 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 NleGD9836 viable Nlek13714 Sterility Allele male sterile Nlek13714 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 NleGD9836 wild-type Nle+t15 Phenotype manifest in Allele mesothoracic tergum, with Scer\GAL4pnr-MD237 NleGD9836 microchaeta, with Scer\GAL4ap-md544 NleScer\UAS.cRa trichogen cell, with Scer\GAL4pnr-MD237 NleGD9836 wing hair | supernumerary, with Scer\GAL4ap-md544 NleGD9836
Classical Alleles ( 3 )
For All Classical Alleles Show
Allele of Nle Class Mutagen Stocks Known lesion Nlek13714 loss of function allele P-element activity 2 Yes Nle5-SZ-3596 1 -- NleΔ8 loss of function allele P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nle Class Mutagen Stocks Known lesion NleGD9836 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Nle+t15 in vitro construct - genomic fragment 0 Yes NleKK102603 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes NleMtnA.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes NleScer\UAS.cRa in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2L)BSC107 (Deal and Cook, 2005.3.10) Df(2L)Exel8003 (Ryder, 2004.4.26) Df(2L)NleΔ8 (Royet et al., 1998)
Not disrupted in	Df(2L)al (Royet et al., 1998, Spradling et al., 1999, BDGP Project Members, 1994-1999) Df(2L)ast1 (Royet et al., 1998, BDGP Project Members, 1994-1999) Df(2L)ast2 (Spradling et al., 1999)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD9836} NA P{KK102603} NA P{UAS-Nle.R} NA characterization construct P{Nle+.Y2-6} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}Nlek13714 No miscellaneous insertions P{RS5}Nle5-SZ-3596 NA
Gene Ontology: Function, Process & Cellular Component ( 2 unique terms )
Terms Based on Experimental Evidence ( 0 terms )
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
Notch binding traceable author statement (Kadesch, 2000)
Biological Process
CV term Evidence References
Notch signaling pathway inferred from sequence or structural similarity with EMBL:AF069737 (FlyBase, 1992-) traceable author statement (Kadesch, 2000)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Nle - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Arf79F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Arp1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Atx2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG12163 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG12264 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG15220 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG15735 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG31694 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG4169 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG4611 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG5525 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG6617 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG6724 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG7033 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG7194 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG8258 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG8801 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - CG9775 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Cct5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Cctγ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Cnx99A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Cyt-c-p anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Dmn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - DnaJ-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Droj2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Hsp22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Hsp23 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Nle - Hsp27 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Ip259 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Nle - Jra anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - LRP1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - LanB1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Mec2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Ns1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Ns2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Prosα7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Pvf2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Rab6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - RpL35A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - RpS10b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - RpS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - RpS19a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Rpt1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Rpt2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Rrp46 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Tcp-1ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - Tcp-1η anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - dpn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - eIF-2β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - eIF6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - hyd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - mRpL3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - qkr58E-3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - slbo anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Nle - tyf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nle allele Gene References Nlek13714 dx (Royet et al., 1998) N (Royet et al., 1998) NleScer\UAS.cRa N (Royet et al., 1998) NleΔ8 dx (Royet et al., 1998) N (Royet et al., 1998) unspecified gro (Royet et al., 1998) Su(H) (Royet et al., 1998)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 59490 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0021874 DroID - A comprehensive database of gene and protein interactions. • FBgn0021874 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33234 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O96995
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6M39V7 Dipteran inclusive ortholog search EOG64F6JF Insect inclusive ortholog search EOG69W19Q Arthropod inclusive ortholog search EOG6280H0 Metazoa inclusive ortholog search EOG6996T5
Orthologs in Drosophila Species (EOG6M39V7)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Nle     Drosophila simulans   Dsim\GD22949 Y   Drosophila sechellia   Dsec\GM16656 Y   Drosophila erecta   Dere\GG24639 Y   Drosophila yakuba   Dyak\GE16018 Y   Drosophila ananassae   Dana\GF14879 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA25887 Y   Drosophila persimilis   Dper\GL19252 Y   Drosophila willistoni   Dwil\GK24428 Y   Drosophila virilis   Dvir\GJ19693 Y   Drosophila mojavensis   Dmoj\GI18023 Y   Drosophila grimshawi   Dgri\GH10505 Y
Orthologs in non-Drosophila Dipterans (EOG64F6JF)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004063   Anopheles gambiae Malaria mosquito  Agam\AGAP002221   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ009423
Orthologs in non-Dipteran Insects (EOG69W19Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB42973   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG012142   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13109   Atta cephalotes Leafcutter ant  Acep\ACEP12286   Camponotus floridanus Florida carpenter ant  Cflo\CFLO21804   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL20137   Linepithema humile Argentine ant  Lhum\LH13506   Pogonomyrmex barbatus Red harvester ant  Pbar\PB13232   Solenopsis invicta Red fire ant  Sinv\SINV17179   Acyrthosiphon pisum Pea aphid  Apim\ACYPI003790   Bombyx mori Silkmoth  Bmor\BGIBMGA005312   Pediculus humanus Human body louse  Phum\PHUM233630   Tribolium castaneum Red flour beetle  Tcas\Nle
Orthologs in non-Insect Arthropods (EOG6280H0)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_300951   Ixodes scapularis Deer tick  Isca\ISCW015750
Orthologs in non-Arthropod Metazoa (EOG6996T5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\W07E6.2   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_004154gn   Danio rerio Zebrafish  Drer\nle1   Xenopus tropicalis Western clawed frog  Xtro\nle1   Gallus gallus Domestic chicken  Ggal\NLE1   Mus musculus House mouse  Mmus\Nle1   Rattus norvegicus Norway rat  Rnor\Nle1   Homo sapiens Human  Hsap\NLE1
Human Orthologs (1)
Gene OMIM HGNC Hsap\NLE1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD22949 Drosophila sechellia Dsec\GM16656 Drosophila erecta Dere\GG24639 Drosophila yakuba Dyak\GE16018 Drosophila ananassae Dana\GF14879 Drosophila pseudoobscura pseudoobscura Dpse\GA25887 Drosophila persimilis Dper\GL19252 Drosophila willistoni Dwil\GK24428 Drosophila virilis Dvir\GJ19693 Drosophila mojavensis Dmoj\GI18023 Drosophila grimshawi Dgri\GH10505
Stocks & Reagents
Stocks Listed in FlyBase ( 5 )
Bloomington	11099 y1 w67c23; P{lacW}Nlek13714 CG2807k13714/CyO
Kyoto	126149 w1118; P{RS5}Nle5-SZ-3596 111330 yd2 w1118 P{ey-FLP.N}2 P{GMR-lacZ.C(38.1)}TPN1; P{lacW}Nlek13714 P{neoFRT}40A/CyO y+
VDRC	v33573 w1118; P{GD9836}v33573 v33574 w1118; P{GD9836}v33574
Genomic Clones ( 1 )
	BACR48E08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 23 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	AT08344
Other clones	DPiM_AT08344
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM08866 GM10004 LD38001 LD40082 RE28535 RE28559 SD16515 SD17257 SD19506 SD22291 SD26605
Other clones	AT01140 CK00074 EK121848 EK171546 EK199146 EP15762 EP18144 FGM222B01 GH19473 UT01504
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0021874 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33234
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Nle CG2863 (Guan, 1999.11)
Source for database merge of	Source for merge of: Nle anon-WO0026364 (FlyBase, 1996-)
Additional comments	Source for merge of Nle anon-WO0026364 was sequence comparison (date:030908). (FlyBase, 1996-)
Other Comments
	The Nle gene product modulates N signalling activity. (Royet et al., 1998) The Nle gene product physically interacts with the N gene product cytoplasmic domain. In direction of increasing cytology: Nle- CG2807+ (Royet et al., 1998) Loss of function mutations dominantly suppress the wing notching caused by alleles of N. Mutants show no interaction with Ser, Dl, H or sno. (Royet et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057946 RefSeq (Proteins) • NP_477294 DDBJ / EMBL / GenBank • AAF51479 AAL90024 AC004573 AC008002 AJ012588 AQ026066 AX025192 AY089286 BF505998 CAA10070 Entrez Gene - A searchable database of RefSeq genes. • 33234 UniProtKB/TrEMBL • O96995 Q9VPR4
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • WD40/YVTN repeat-like-containing domain (IPR015943) WD40-repeat-containing domain (IPR017986) G-protein, beta subunit (IPR001632) WD40 repeat (IPR001680) NLE (IPR012972) WD40 repeat, conserved site (IPR019775) G-protein beta WD-40 repeat (IPR020472) Quinonprotein alcohol dehydrogenase-like superfamily (IPR011047) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • AT08344
Linkouts
	BioGRID - A database of protein and genetic interactions • 59490 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0021874 DroID - A comprehensive database of gene and protein interactions. • FBgn0021874 DRSC - Results from RNAi screens. • FBgn0021874 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0021874 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG2863-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0021874 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 33234 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 33234 modMine - Data generated by the modENCODE project. • FBgn0021874 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O96995
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	anon-WO0026364   CG2863 (Keleman et al., 2009.8.5, Weber et al., 2012) l(2)k13714 (Spradling et al., 1999, ) Nle (Weber et al., 2012)
Name Synonym	notchless (Royet, 1998.10.28) Notchless (Royet et al., 1998)
Secondary FlyBase IDs
	FBgn0042663
References ( 28 )
Generate a list of	All references relating to this gene ( 28 )
List References by type	Research paper  ( 5 ) Supplementary material  ( 5 ) Review  ( 2 ) Personal communication to FlyBase  ( 7 ) FlyBase analysis  ( 3 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 ) Patent  ( 1 )
Recent research papers ( 1 )
	Weber et al., 2012, Genetics 191(1): 145--162 Novel regulators of planar cell polarity: a genetic analysis in Drosophila. [FBrf0218210] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...