FB2020_03, released Jun 16, 2020

Gene: Dmel\eIF3h

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General Information
Symbol
Dmel\eIF3h
Species
D. melanogaster
Name
eukaryotic translation initiation factor 3 subunit h
Annotation Symbol
CG9124
Feature Type
protein_coding_gene
FlyBase ID
FBgn0022023
Gene Model Status
Current
Stock Availability
12 publicly available
Gene Snapshot
Contributions welcome
Other Summaries
Also Known As

eIF-3p40, l(2)k09003, Eukaryotic initiation factor 3 p40 subunit

Key Links
Genomic Location
Cytogenetic map
25C3-25C3
Sequence location
2L:5,051,193..5,053,094 [-]
Recombination map

2-15

RefSeq locus
NT_033779 REGION:5051193..5053094
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (8 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
metallopeptidase activity
inferred from biological aspect of ancestor with PANTHER:PTN000045368
(assigned by GO_Central )
(Gaudet et al., 2011)
translation initiation factor activity
inferred from sequence or structural similarity
(Lasko, 2000)
contributes_to translation initiation factor activity
inferred from biological aspect of ancestor with PANTHER:PTN000045506
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (2 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
positive regulation of Notch signaling pathway
inferred from mutant phenotype
(Zhang et al., 2012)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
translational initiation
inferred from sequence or structural similarity
(Lasko, 2000)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
cytosol
inferred from sequence or structural similarity
(Lasko, 2000)
inferred from biological aspect of ancestor with PANTHER:PTN000045506
(assigned by GO_Central )
(Gaudet et al., 2011)
eukaryotic 43S preinitiation complex
inferred from biological aspect of ancestor with PANTHER:PTN000045506
(assigned by GO_Central )
(Gaudet et al., 2011)
eukaryotic translation initiation factor 3 complex
inferred from biological aspect of ancestor with PANTHER:PTN000045506
(assigned by GO_Central )
(Gaudet et al., 2011)
inferred from sequence or structural similarity
(Lasko, 2000)
polysomal ribosome
inferred from biological aspect of ancestor with PANTHER:PTN000045506
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
Belongs to the eIF-3 subunit H family. (Q9U9Q4)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
JAMM DEUBIQUITINASES -
The JAMM (JAB1/MPN/MOV34) sub-family of deubiquitinases are Zn2+-binding metalloproteases. In general, JAMMs are components of larger assemblies such as the proteasome and COP9 signalosome. Several members of this family lack the ability to co-ordinate Zn2+ and therefore enzymatically inactive; they may act as scaffold proteins or as ubiquitin-binding modules within complexes. (Adapted from PMID:23899565 and PMID:17559875).
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Pathway (FlyBase)
Positive Regulators of Notch Signaling Pathway -
The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
Protein Function (UniProtKB)
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation.
(UniProt, Q9U9Q4)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
1

Please see the GBrowse view of Dmel\eIF3h or the JBrowse view of Dmel\eIF3h for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
eIF3h-RA
FBtr0079030
NM_080095
1496
338
eIF3h-RB
FBtr0079031
NM_205909
1182
338
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
eIF3h-PA
FBpp0078667
38.4
338
6.01
NP_524834
AAF52210
eIF3h-PB
FBpp0089133
38.4
338
6.01
NP_995631
AAS64639
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

338 aa isoforms: eIF3h-PA, eIF3h-PB
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt (PubMed:23716590).

(UniProt, Q9U9Q4)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3h using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 3
organism

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 9 -- 10
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 11 -- 12
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 13 -- 16
organism | ubiquitous
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3h in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF3h
Transgenic constructs containing regulatory region of eIF3h
Deletions and Duplications ( 5 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\EIF3H
13 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Eif3h
13 of 15
Yes
Yes
Rattus norvegicus (Norway rat) (3)
Rnor\LOC100911110
11 of 13
Yes
Yes
Rnor\Eif3h
7 of 13
No
Yes
Rnor\LOC108348062
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\eif3h
13 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\eif3hb
14 of 15
Yes
Yes
Drer\eif3ha
13 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\eif-3.H
14 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\TIF3H1
9 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPAC821.05
10 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190AST )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000268
Drosophila simulans
Dsim\GD14929
Drosophila sechellia
Dsec\GM18040
Drosophila erecta
Dere\GG24322
Drosophila yakuba
Dyak\GE18701
Drosophila ananassae
Dana\GF14158
Drosophila pseudoobscura pseudoobscura
Dpse\GA28878
Drosophila persimilis
Dper\GL26477
Drosophila willistoni
Dwil\GK24148
Drosophila virilis
Dvir\GJ10133
Drosophila mojavensis
Dmoj\GI15660
Drosophila grimshawi
Dgri\GH10269
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG0915077J )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17010398
Glossina morsitans
Tsetse fly
Gmor\GMOY005201
Glossina morsitans
Tsetse fly
Gmor\GMOY003595
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_03935
Mayetiola destructor
Hessian fly
Mdes\Mdes004637
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007945
Anopheles darlingi
American malaria mosquito
Adar\ADAC008191
Anopheles gambiae
Malaria mosquito
Agam\AGAP009204
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ005299
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W06X4 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA006141
Danaus plexippus
Monarch butterfly
Dple\DPOGS207638-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL011986
Apis florea
Little honeybee
Aflo\2365730644
Apis mellifera
Western honey bee
Amel\GB46247
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP23675
Bombus terrestris
Buff-tailed bumblebee
Bter\15205766
Linepithema humile
Argentine ant
Lhum\LH25061
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG004157
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_07146
Tribolium castaneum
Red flour beetle
Tcas\TC014327
Pediculus humanus
Human body louse
Phum\PHUM509090
Rhodnius prolixus
Kissing bug
Rpro\RPRC013955
Cimex lectularius
Bed bug
Clec\CLEC005460
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009633
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_12335
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X06SH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR003784
Ixodes scapularis
Black-legged tick
Isca\ISCW018927
Stegodyphus mimosarum
African social velvet spider
Smim\KFM64145.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM69907.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur16g03110
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_305161
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0E5O )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Eif3s3L
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000010830
Gallus gallus
Domestic chicken
Ggal\EIF3H
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (2)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
CSN5
2 of 10
Rpn11
2 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    EIF3H; eukaryotic translation initiation factor 3 subunit H
    13 of 15
    603912
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Subunit Structure (UniProtKB)
        Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt (PubMed:23716590).
        (UniProt, Q9U9Q4 )
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Positive Regulators of Notch Signaling Pathway -
        The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Positive regulators of the pathway enhance the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).
        Metabolic Pathways
        External Data
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        2L
        Recombination map

        2-15

        Cytogenetic map
        25C3-25C3
        Sequence location
        2L:5,051,193..5,053,094 [-]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        25C3-25C3
        Limits computationally determined from genome sequence between P{lacW}vkgk16721&P{lacW}Cg25Ck00405 and P{lacW}l(2)k11206k11206
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        25C1-25C2
        (determined by in situ hybridisation)
        (Spradling et al., 1999)
        25C1-25C2
        (BDGP Project Members, 1994-1999)
        Experimentally Determined Recombination Data
        Location

        2-15

        (FlyBase, 2016)

        2-16.5

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (12)
        Genomic Clones (14)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (94)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
          Drosophila Genomics Resource Center cDNA clones

          For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

          cDNA Clones, End Sequenced (ESTs)
          BDGP DGC clones
          Other clones
          RNAi and Array Information
          Linkouts
          DRSC - Results frm RNAi screens
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          Antibody Information
          Laboratory Generated Antibodies
           
          Commercially Available Antibodies
           
          Other Information
          Relationship to Other Genes
          Source for database identify of

          Source for identity of: BcDNA:GM14618 CG9124

          (Kodira, 1999.11)

          Source for identity of: eIF3h eIF-3p40

          (Marygold et al., 2017)
          Source for database merge of

          Source for merge of: eIF-3p40 BcDNA:GM14618

          (Misra, 2000.8.9)
          Additional comments
          Other Comments
          Origin and Etymology
          Discoverer
          Etymology
          Identification
          External Crossreferences and Linkouts ( 42 )
          Sequence Crossreferences
          NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
          GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
          GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
          RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
          UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
          Other crossreferences
          BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
          Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
          Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
          Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
          Flygut - An atlas of the Drosophila adult midgut
          GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
          iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
          InterPro - A database of protein families, domains and functional sites
          KEGG Genes - Molecular building blocks of life in the genomic space.
          modMine - A data warehouse for the modENCODE project
          Linkouts
          BioGRID - A database of protein and genetic interactions.
          DroID - A comprehensive database of gene and protein interactions.
          DRSC - Results frm RNAi screens
          FLIGHT - Cell culture data for RNAi and other high-throughput technologies
          FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
          FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
          FlyMine - An integrated database for Drosophila genomics
          InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
          KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
          MIST (protein-protein) - An integrated Molecular Interaction Database
          Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
          Synonyms and Secondary IDs (11)
          Datasets (0)
          Study focus (0)
          Experimental Role
          Project
          Project Type
          Title
          References (68)