FlyBase Gene Report: Dmel\Pp2C1

General Information

Symbol

Dmel\Pp2C1

Species

D. melanogaster

Name

Protein phosphatase 2C

Annotation Symbol

CG2984

Feature Type

protein_coding_gene

FlyBase ID

FBgn0022768

Gene Model Status

Current

Stock Availability

13 publicly available

Enzyme Name (EC)

protein-serine/threonine phosphatase (3.1.3.16)

Gene Summary

Contribute a Gene Snapshot for this gene.

All Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

PP2C, dpp2c1

Key Links

Genomic Location

Cytogenetic map

4C12-4C13

Sequence location

X:4,679,988..4,686,903 [-]

Recombination map

1-8

RefSeq locus

NC_004354 REGION:4679988..4686903

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (7 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

enables cation binding

inferred from electronic annotation with InterPro:IPR000222

(InterPro Project Members, 2004-)

enables mitogen-activated protein kinase binding

inferred from biological aspect of ancestor with PANTHER:PTN001014085

(GO Reference Genome Project, 2011-)

enables oxidoreductase activity

inferred from electronic annotation with InterPro:IPR003952

(InterPro Project Members, 2004-)

enables protein serine/threonine phosphatase activity

inferred from sequence or structural similarity with SGD:S000000329

(Gene Ontology Curators, 2002-)

inferred from biological aspect of ancestor with PANTHER:PTN008268578

(GO Reference Genome Project, 2011-)

inferred from electronic annotation with InterPro:IPR015655

(InterPro Project Members, 2004-)

Biological Process (2 terms)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in negative regulation of gene expression, epigenetic

inferred from biological aspect of ancestor with PANTHER:PTN001014085

(GO Reference Genome Project, 2011-)

involved_in regulation of intracellular signal transduction

inferred from biological aspect of ancestor with PANTHER:PTN008726241

(GO Reference Genome Project, 2011-)

Cellular Component (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

located_in cytoplasm

inferred from sequence or structural similarity with SGD:S000000329

(Gene Ontology Curators, 2002-)

Gene Group (FlyBase)

PPM FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES

Protein Family (UniProt)

Protein Signatures (InterPro)

Catalytic Activity (EC/Rhea)

protein serine/threonine phosphatase activity

(1) O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate (3.1.3.16)

(2) O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate (3.1.3.16)

Summaries

Gene Group (FlyBase)

PPM FAMILY PROTEIN SERINE/THREONINE PHOSPHATASES -: Metal-dependent protein phosphatase (PPM) are Mg²⁺- or Mn²⁺-dependent serine/threonine phosphatases. The phosphatase does not require regulatory subunits, but has additional domains that may determine substrate specificity. (Adapted from PMID:11230548 and PMID:19879837).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Pp2C1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q9W4H8)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.52

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Pp2C1-RA

FBtr0070685

NM_080335

5259

1427

Pp2C1-RB

FBtr0343531

NM_001297938

5675

1427

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Pp2C1-PA

FBpp0070653

153.9

1427

5.04

FBpp0310135

153.9

1427

5.04

Q9W4H8

NP_001284867

AHN59338

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1427 aa isoforms: Pp2C1-PA, Pp2C1-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Pp2C1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pp2C1_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.60

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Pp2C1 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Pp2C1

View exonic expression by tissue for Dmel\Pp2C1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31404/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0022768

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0022768

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0022768

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2984

Flygut - An atlas of the Drosophila adult midgut

FBgn0022768

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 6 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 9 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Pp2C1

Transgenic constructs containing regulatory region of Pp2C1

Aberrations (Deficiencies and Duplications) ( 0 )

Inferred from experimentation ( 0 )

Inferred from location ( 18 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

viable, with Scer\GAL4^Mef2.PR

Pp2C1^GD9874

viable, with Scer\GAL4^pnr-MD237

Pp2C1^GD9874

Sterility

Allele

female semi-sterile, with Scer\GAL4^{VP16.mat.αTub67C}

Pp2C1^HMS01887

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

Pp2C1^GD9874

Phenotype manifest in

Allele

trichogen cell, with Scer\GAL4^pnr-MD237

Pp2C1^GD9874

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (18)

Hsap\PPM1D

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (17)

Rnor\Ppm1d

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (18)

Mmus\Ppm1d

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (10)

Xtro\ppm1d

6 of 13

Yes

Xtro\drg2

1 of 13

Xtro\ilkap

1 of 13

Xtro\islr2

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (22)

Drer\ppm1da

9 of 14

Yes

Drer\ppm1db

9 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (10)

Cele\ppm-1.D

9 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (11)

Agam\AgaP_AGAP004291

7 of 12

Yes

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (82)

Atha\AHG1

3 of 13

Yes

Atha\AT1G03590

3 of 13

Yes

Atha\AT1G09160

3 of 13

Yes

Atha\AT1G16220

3 of 13

Yes

Atha\AT1G43900

3 of 13

Yes

Atha\AT1G47380

3 of 13

Yes

Atha\AT1G68410

3 of 13

Yes

Atha\AT1G79630

3 of 13

Yes

Atha\AT2G34740

3 of 13

Yes

Atha\AT2G40860

3 of 13

Yes

Atha\AT3G05640

3 of 13

Yes

Atha\AT3G12620

3 of 13

Yes

Atha\AT3G17090

3 of 13

Yes

Atha\AT3G51370

3 of 13

Yes

Atha\AT3G55050

3 of 13

Yes

Atha\AT3G63320

3 of 13

Yes

Atha\AT4G28400

3 of 13

Yes

Atha\AT4G32950

3 of 13

Yes

Atha\AT4G33920

3 of 13

Yes

Atha\AT4G38520

3 of 13

Yes

Atha\AT5G02760

3 of 13

Yes

Atha\AT5G06750

3 of 13

Yes

Atha\AT5G10740

3 of 13

Yes

Atha\AT5G24940

3 of 13

Yes

Atha\AT5G26010

3 of 13

Yes

Atha\AT5G27930

3 of 13

Yes

Atha\AT5G66080

3 of 13

Yes

Atha\HAI1

3 of 13

Yes

Atha\HAI2

3 of 13

Yes

Atha\HAI3

3 of 13

Yes

Atha\PIA1

3 of 13

Yes

Atha\PP2CA

3 of 13

Yes

Atha\WIN2

3 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

Yes

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\PTC1

2 of 13

Yes

Scer\PTC2

2 of 13

Yes

Scer\PTC3

2 of 13

Yes

Scer\PTC4

2 of 13

Yes

Scer\PTC5

2 of 13

Yes

Scer\PTC6

1 of 13

Scer\PTC7

1 of 13

Schizosaccharomyces pombe (Fission yeast) (6)

Spom\ptc1

2 of 12

Yes

Spom\ptc2

2 of 12

Yes

Spom\ptc3

2 of 12

Yes

Spom\azr1

1 of 12

Spom\ptc4

1 of 12

Spom\SPAC10F6.17c

1 of 12

Escherichia coli (enterobacterium) (1)

Ecol\pphC

1 of 11

Yes

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Pp2C1. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (13)

alph

4 of 13

Nil

4 of 13

3 of 13

3 of 13

3 of 13

3 of 13

3 of 13

2 of 13

Pdp

2 of 13

CG12091

1 of 13

CG15035

1 of 13

fig

1 of 13

Map205

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

PPM1D; protein phosphatase, Mg2+/Mn2+ dependent 1D

model of breast cancer

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

PPM1D; protein phosphatase, Mg2+/Mn2+ dependent 1D

9 of 14

605100

breast cancer

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Other Interaction Browsers

Summary of Genetic Interactions

esyN Network Diagram

Other Interaction Browsers

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

57919

DroID - A comprehensive database of gene and protein interactions.

FBgn0022768

MIST (protein-protein) - An integrated Molecular Interaction Database

31404

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Transcriptional regulation by RUNX2

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

1-8

Cytogenetic map

4C12-4C13

Sequence location

X:4,679,988..4,686,903 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

4C12-4C13

Limits computationally determined from genome sequence between P{EP}CG2930^EP1352 and P{EP}EP425

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

4E1-4E2

(Dick, 1997.4.7, Dick et al., 1997)

Experimentally Determined Recombination Data

Location

1-8

(FlyBase, 2016)

1-7.6

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (32)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

LD34192

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0022768

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0022768

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Pp2C1 has been cloned and sequenced.

(Dick et al., 1997)

Relationship to Other Genes

Source for database merge of

Source for merge of: Pp2C1 anon-WO03040301.132

(FlyBase, 1996-)

Additional comments

Source for merge of Pp2C1 anon-WO03040301.132 was sequence comparison ( date:051113 ).

(FlyBase, 1996-)

Nomenclature History

Source for database identify of

Source for identity of: Pp2C1 CG2984

(Zhao, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

CG2984

(Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Quinones-Coello, 2007, Legube et al., 2006, Mount, 2001.8.14, Morrison et al., 2000)

PP2C

(Gonda and Stronach, 2007, Wharton, 2003)

PP2C1

(Morrison et al., 2000)

Pp2C1

(Chang et al., 2022, Bilder et al., 2021, Ostalé et al., 2021, Valsecchi et al., 2018, Transgenic RNAi Project members, 2017-, Ashwal-Fluss et al., 2014, Sopko et al., 2014, Kwon et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Wasbrough et al., 2010, Quinones-Coello, 2007)

anon-WO03040301.132

dpp2c1

(Dick, 1997.4.7, Dick et al., 1997)

Name Synonyms

Protein phosphatase 2C

(Dick et al., 1997)

Secondary FlyBase IDs

FBgn0066851

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 44 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

31404

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q9W4H8

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q9W4H8

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

31404/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0022768

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0022768

FlyMine - An integrated database for Drosophila genomics

FBgn0022768

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG2984

MARRVEL_MODEL - MARRVEL (model organism gene)

31404

Linkouts

BioGRID - A database of protein and genetic interactions.

57919

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0022768

DroID - A comprehensive database of gene and protein interactions.

FBgn0022768

DRSC - Results frm RNAi screens

FBgn0022768

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Pp2C1_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0022768

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0022768

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG2984

Flygut - An atlas of the Drosophila adult midgut

FBgn0022768

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0022768

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC034727

MIST (protein-protein) - An integrated Molecular Interaction Database

31404

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Transcriptional regulation by RUNX2

References (53)

The following external sites may use different assemblies or annotations than FlyBase.

1.60

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