FB2020_01, released Feb 12, 2020

Gene: Dmel\Uba1

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General Information
Symbol
Dmel\Uba1
Species
D. melanogaster
Name
Ubiquitin activating enzyme 1
Annotation Symbol
CG1782
Feature Type
protein_coding_gene
FlyBase ID
FBgn0023143
Gene Model Status
Current
Stock Availability
21 publicly available
Enzyme Name (EC)
E1 ubiquitin-activating enzyme (6.2.1.45)
Gene Snapshot
Ubiquitin activating enzyme 1 (Uba1) encodes an E1 enzyme involved in protein ubiquitylation. It contributes to multiple processes including regulation of Ras-ERK signaling, autophagy, apoptosis and tissue growth. [Date last reviewed: 2019-07-11]
Other Summaries
Also Known As

E1, UBA-1, l(2)03405, Ubiquitin Activating Enzyme, Uba 1

Key Links
Genomic Location
Cytogenetic map
46A1-46A1
Sequence location
2R:9,688,289..9,693,328 [-]
Recombination map

2-61

RefSeq locus
NT_033778 REGION:9688289..9693328
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
ubiquitin activating enzyme activity
Predictions / Assertions
ubiquitin activating enzyme activity
Catalytic Activity (EC)
Experimental Evidence
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine (6.2.1.45)
Predictions / Assertions
ATP + ubiquitin + [E1 ubiquitin-activating enzyme]-L-cysteine = AMP + diphosphate + S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine (6.2.1.45)
Summaries
Gene Group (FlyBase)
UBIQUITIN ACTIVATING ENZYMES -
Ubiquitin activating enzymes are 'canonical' E1 enzymes that activate ubiquitin for subsequent steps along the ubiquitin conjugation cascade. They perform three distinct chemical reactions: first, they bind ATP, magnesium and ubiquitin to catalyze formation of a high-energy acyl adenylate intermediate with subsequent release of pyrophosphate; second, an E1 catalytic cysteine attacks the ubiquitin-adenylate to catalyze formation of a high-energy thioester bond between the E1 and ubiquitin and release of the AMP product; finally, the E1-ubiquitin catalyzes transthioesterification to an E2 to form a high energy thioester-linked E2-ubiquitin product. (Adapted from PMID:28234446.)
Summary (Interactive Fly)

Ubiquitin-activating enzyme, E1, activates and transfers ubiquitin to ubiquitin-conjugating enzymes, tumor suppressor

Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Uba1 or the JBrowse view of Dmel\Uba1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Uba1-RA
FBtr0088499
NM_057962
4343
1191
Uba1-RC
FBtr0339517
NM_001273902
4343
1008
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
UniProt
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Uba1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (20 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
ubiquitin activating enzyme activity
inferred from direct assay
(Chang et al., 2013)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
ubiquitin activating enzyme activity
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (15 terms)
Terms Based on Experimental Evidence (12 terms)
CV Term
Evidence
References
autophagy
inferred from mutant phenotype
(Chang et al., 2013)
glucose homeostasis
inferred from mutant phenotype
(Ugrankar et al., 2015)
larval midgut cell programmed cell death
inferred from mutant phenotype
(Chang et al., 2013)
lipid storage
inferred from direct assay
(Beller et al., 2008)
mushroom body development
inferred from mutant phenotype
(Schuldiner et al., 2008)
neuron remodeling
inferred from mutant phenotype
(Watts et al., 2003)
ovarian follicle cell development
inferred from mutant phenotype
(Jia et al., 2015)
protein polyubiquitination
inferred from mutant phenotype
(Lee et al., 2014)
protein ubiquitination
inferred from direct assay
(Chang et al., 2013)
inferred from mutant phenotype
(Yan et al., 2009, Lee et al., 2008)
regulation of growth
inferred from mutant phenotype
(Lee et al., 2008)
regulation of programmed cell death
inferred from mutant phenotype
(Lee et al., 2008)
inferred from genetic interaction with FLYBASE:hid; FB:FBgn0003997
(Lee et al., 2008)
regulation of Ras protein signal transduction
inferred from mutant phenotype
(Yan et al., 2009)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
cellular response to DNA damage stimulus
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(assigned by GO_Central )
(Gaudet et al., 2011)
protein modification by small protein conjugation
inferred from biological aspect of ancestor with PANTHER:PTN000102039
(assigned by GO_Central )
(Gaudet et al., 2011)
protein ubiquitination
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(assigned by GO_Central )
(Gaudet et al., 2011)
ubiquitin-dependent protein catabolic process
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(assigned by GO_Central )
(Gaudet et al., 2011)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
dendrite
inferred from mutant phenotype
(Kuo et al., 2005)
plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000102040
(assigned by GO_Central )
(Gaudet et al., 2011)
nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000102404
(assigned by GO_Central )
(Gaudet et al., 2011)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
prepupal stage P1
gamma Kenyon cell
(Hoopfer et al., 2008)
late third instar larval stage
gamma Kenyon cell | faint
(Hoopfer et al., 2008)
embryonic stage 1 -- 3
organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)
embryonic stage 4 -- 6
organism | ubiquitous
(Fisher et al., 2012)
embryonic stage 7 -- 8
dorsal ectoderm
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
embryonic stage 9 -- 10
antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)
head mesoderm
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
trunk mesoderm
(Fisher et al., 2012)
ventral ectoderm
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 11 -- 12
central brain primordium
(Fisher et al., 2012)
embryonic central brain glial cell
(Fisher et al., 2012)
embryonic central brain neuron
(Fisher et al., 2012)
hindgut proper primordium
(Fisher et al., 2012)
lateral cord glial cell
(Fisher et al., 2012)
lateral cord neuron
(Fisher et al., 2012)
Malpighian tubule primordium
(Fisher et al., 2012)
organism | ubiquitous
(Fisher et al., 2012)
ventral nerve cord primordium
(Fisher et al., 2012)
embryonic stage 13 -- 16
embryonic brain
(Fisher et al., 2012)
embryonic hindgut
(Fisher et al., 2012)
embryonic/larval midgut
(Fisher et al., 2012)
embryonic/larval nervous system
(Fisher et al., 2012)
gonad
(Fisher et al., 2012)
ventral nerve cord
(Fisher et al., 2012)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Wasbrough et al., 2010)
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
dendrite
inferred from mutant phenotype
(Kuo et al., 2005)
plasma membrane
inferred from high throughput direct assay
(Tan et al., 2009)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Uba1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 43 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 11 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Uba1
Transgenic constructs containing regulatory region of Uba1
Deletions and Duplications ( 2 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone | heat sensitive
lethal
lethal (with Uba103405)
lethal (with Uba1D6)
lethal (with Uba1H33)
lethal (with Uba1H42)
lethal (with Uba1s3484)
lethal (with Uba1UY3010)
lethal | recessive
lethal - all die before end of P-stage | recessive
lethal - all die before end of pupal stage
partially lethal - majority die
viable, with Scer\GAL4da.G32
viable, with Scer\GAL4ptc.PU
viable, with Scer\GAL4vg.PM
viable (with Uba1s3484), with Scer\GAL4da.G32
viable (with Uba1UY3010), with Scer\GAL4da.G32
Sterility
Allele
sterile
sterile (with Uba1B1)
sterile (with Uba1B2)
Other Phenotypes
Allele
chemical sensitive, with Scer\GAL4elav.PU
decreased cell death | pupal stage | somatic clone
decreased occurrence of cell division | larval stage | somatic clone
exocytosis defective | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
increased cell death | pupal stage | somatic clone | heat sensitive
increased cell number | cell non-autonomous | somatic clone
increased occurrence of cell division | cell non-autonomous | larval stage | somatic clone
increased occurrence of cell division | larval stage (with Uba1B1)
increased occurrence of cell division | larval stage (with Uba1B2)
locomotor behavior defective
locomotor behavior defective (with Uba1B1)
locomotor behavior defective (with Uba1B2)
neuroanatomy defective | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
neuroanatomy defective | larval stage, with Scer\GAL4elav-C155
neuroanatomy defective | pupal stage
neuroanatomy defective | somatic clone
short lived, with Scer\GAL4elav.PU
short lived | dominant
size defective | adult stage
some die during pupal stage
visible (with Uba1B1)
visible (with Uba1B2)
visible | recessive
visible | somatic clone
visible | somatic clone | heat sensitive
Phenotype manifest in
Allele
adult mushroom body | somatic clone
anterior fascicle & synapse, with Scer\GAL4elav-C155
anterior fascicle & synapse | supernumerary, with Scer\GAL4elav-C155
axon | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
axon | adult stage | somatic clone
dendrite | somatic clone
dendritic arborizing neuron | P-stage
dopaminergic neuron | chemical conditional, with Scer\GAL4elav.PU
dorsocentral bristle
embryonic/larval fat body | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
extracellular matrix | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
eye | cell non-autonomous | somatic clone
eye | somatic clone
eye | somatic clone | heat sensitive
eye-antennal disc | somatic clone | third instar larval stage | heat sensitive
eye disc | cell non-autonomous | somatic clone
eye disc | pupal stage | somatic clone
eye disc | somatic clone
eye disc posterior to the morphogenetic furrow
eye disc posterior to the morphogenetic furrow (with Uba1B1)
eye disc posterior to the morphogenetic furrow (with Uba1B2)
gamma Kenyon cell | adult stage, with Scer\GAL4GMR71G10, Spyo\Cas9UAS.Tag:NLS(Unk)
humeral disc
interommatidial cell | pupal stage | somatic clone
intersegmental nerve, with Scer\GAL4elav-C155
leg
macrochaeta
mushroom body & axon | somatic clone
neuroblast | somatic clone
proboscis
retina | P-stage | somatic clone
sternopleural bristle | supernumerary
wing
wing vein
wing vein (with Uba1B1)
wing vein (with Uba1B2)
wing vein | ectopic
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\UBA1
13 of 15
Yes
Yes
Hsap\UBA7
7 of 15
No
Yes
 
Hsap\UBA6
3 of 15
No
Yes
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Uba1
12 of 15
Yes
Yes
Mmus\Uba1y
11 of 15
No
Yes
Mmus\Uba7
8 of 15
No
Yes
Mmus\Uba6
3 of 15
No
Yes
Rattus norvegicus (Norway rat) (6)
Rnor\Uba1
12 of 13
Yes
Yes
Rnor\Uba1y
4 of 13
No
Yes
Rnor\Uba7
4 of 13
No
Yes
Rnor\Uba6
3 of 13
No
Yes
Rnor\LOC103694556
2 of 13
No
Yes
Rnor\LOC103694557
2 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (2)
Xtro\LOC100495145
4 of 12
Yes
Yes
Xtro\uba6
1 of 12
No
Yes
Danio rerio (Zebrafish) (3)
Drer\uba1
13 of 15
Yes
Yes
Drer\uba7
7 of 15
No
Yes
Drer\si:dkey-82j4.2
2 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\uba-1
11 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (2)
Atha\UBA1
8 of 9
Yes
Yes
Atha\UBA 2
8 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\UBA1
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\ptr3
11 of 12
Yes
Yes
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919015Y )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Uba1
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00003377
Drosophila simulans
Dsim\GD10057
Drosophila sechellia
Dsec\GM20586
Drosophila erecta
Dere\GG25268
Drosophila yakuba
Dyak\GE22126
Drosophila ananassae
Dana\GF11487
Drosophila pseudoobscura pseudoobscura
Dpse\GA14681
Drosophila persimilis
Dper\GL17328
Drosophila willistoni
Dwil\GK15620
Drosophila virilis
Dvir\GJ20835
Drosophila mojavensis
Dmoj\GI21231
Drosophila grimshawi
Dgri\GH23328
Drosophila grimshawi
Dgri\GH22854
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500YT )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17019149
Musca domestica
House fly
Mdoa\17019150
Glossina morsitans
Tsetse fly
Gmor\GMOY001369
Mayetiola destructor
Hessian fly
Mdes\Mdes009753
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL000758
Anopheles darlingi
American malaria mosquito
Adar\ADAC008378
Anopheles gambiae
Malaria mosquito
Agam\AGAP011872
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ016556
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ001423
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00RX )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009911
Danaus plexippus
Monarch butterfly
Dple\DPOGS207441-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL012328
Apis florea
Little honeybee
Aflo\2921393092
Apis mellifera
Western honey bee
Amel\Uba1
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP13938
Bombus terrestris
Buff-tailed bumblebee
Bter\15215337
Linepithema humile
Argentine ant
Lhum\LH12261
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:000bdb
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG024134
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_10832
Tribolium castaneum
Red flour beetle
Tcas\TC031473
Pediculus humanus
Human body louse
Phum\PHUM354890
Rhodnius prolixus
Kissing bug
Rpro\RPRC003935
Cimex lectularius
Bed bug
Clec\CLEC025155
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI006009
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_08188
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00QH )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR001087
Ixodes scapularis
Black-legged tick
Isca\ISCW019123
Stegodyphus mimosarum
African social velvet spider
Smim\KFM79356.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur12g01800
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_186898
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0130 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Uba6-2
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Uba6
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Uba1
Ciona intestinalis
Vase tunicate
Cint\LOC100176463
Gallus gallus
Domestic chicken
Ggal\UBA6
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (7)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Aos1
1 of 10
APP-BP1
1 of 10
Atg7
1 of 10
Uba2
1 of 10
Uba3
1 of 10
Uba4
1 of 10
Uba5
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    UBA1; ubiquitin like modifier activating enzyme 1
    13 of 15
    314370
      UBA7; ubiquitin like modifier activating enzyme 7
      7 of 15
      191325
           
          UBA6; ubiquitin like modifier activating enzyme 6
          3 of 15
          611361
              Functional Complementation Data
              Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
              Interactions
              Summary of Physical Interactions
              esyN Network Diagram
              Show neighbor-neighbor interactions:
              Select Layout:
              Legend:
              Protein
              RNA
              Selected Interactor(s)
              Interactions Browser
              View in Interactions Browser
              Please see the Physical Interaction reports below for full details
              protein-protein
              Physical Interaction
              Assay
              References
              Uba1 - ben
              experimental knowledge based
              (Guruharsha et al., 2011)
              Summary of Genetic Interactions
              esyN Network Diagram
              esyN Network Key:
              Suppression
              Enhancement
              View in Interactions Browser
              Please look at the allele data for full details of the genetic interactions
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              Uba1
              suppressible
              Diap1
              (Pfleger et al., 2007)
              Ras85D|Uba1
              suppressible
              egr
              (Yan et al., 2009)
              Uba1
              suppressible
              N
              (Lee et al., 2008)
              Uba1
              suppressible
              Ras85D
              (Liu and Pfleger, 2013, Yan et al., 2009)
              Uba1
              suppressible
              Stat92E
              (Lee et al., 2008)
              Starting gene(s)
              Interaction type
              Interacting gene(s)
              Reference
              bchs
              enhanceable
              Uba1
              (Simonsen et al., 2007)
              Dcp-1
              enhanceable
              Uba1
              (Kim et al., 2010)
              Debcl
              suppressible
              Uba1
              (Colin et al., 2014)
              egr
              suppressible
              Uba1
              (Pfleger et al., 2007)
              grim::rpr
              enhanceable
              Uba1
              (Wing et al., 2002)
              grim
              suppressible
              Uba1
              (Pfleger et al., 2007)
              hid
              suppressible
              Uba1
              (Christiansen et al., 2013, Lee et al., 2008, Pfleger et al., 2007)
              hpo
              enhanceable
              Uba1
              (Pfleger et al., 2007)
              External Data
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DroID - A comprehensive database of gene and protein interactions.
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Pathways
              Signaling Pathways (FlyBase)
              Metabolic Pathways
              External Data
              Linkouts
              KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Genomic Location and Detailed Mapping Data
              Chromosome (arm)
              2R
              Recombination map

              2-61

              Cytogenetic map
              46A1-46A1
              Sequence location
              2R:9,688,289..9,693,328 [-]
              FlyBase Computed Cytological Location
              Cytogenetic map
              Evidence for location
              46A1-46A1
              Limits computationally determined from genome sequence between P{PZ}Mmp202353&P{PZ}Uba105642 and P{lacW}l(2)k09221k09221&P{PZ}dap04454
              Experimentally Determined Cytological Location
              Cytogenetic map
              Notes
              References
              46A-46A
              (Zur Lage, 1997.12.15)
              46A1-46A2
              (determined by in situ hybridisation)
              (Spradling et al., 1999)
              46A1-46A2
              46A2--3 46A2--4 46A3--4
              (BDGP Project Members, 1994-1999)
              Experimentally Determined Recombination Data
              Location

              2-61

              (FlyBase, 2016)

              2-59.5

              (Comeron et al., 2012)
              Left of (cM)
              Right of (cM)
              Notes
              Stocks and Reagents
              Stocks (21)
              Genomic Clones (19)
              cDNA Clones (188)
               

              Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

              cDNA clones, fully sequences
              BDGP DGC clones
              Other clones
              Drosophila Genomics Resource Center cDNA clones

              For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

              cDNA Clones, End Sequenced (ESTs)
              BDGP DGC clones
              Other clones
              RNAi and Array Information
              Linkouts
              DRSC - Results frm RNAi screens
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              Antibody Information
              Laboratory Generated Antibodies
               
              Commercially Available Antibodies
               
              Other Information
              Relationship to Other Genes
              Source for database identify of

              Source for identity of: Uba1 CG1782

              (Evangelista, 1999.11)
              Source for database merge of

              Source for merge of: Uba1 l(2)03405

              (Spradling et al., 1999)

              Source for merge of: Uba1 l(2)05642

              (Misra, 2000.11.18)
              Additional comments
              Other Comments

              Uba1 controls apoptosis autonomously and tissue growth non-autonomously.

              (Lee et al., 2008)

              Uba1 mutations that appear to be hypomorphic elicit a mild overrepresentation of mutant tissue in a mosaic eye, while Uba1 mutations that represent strong or null alleles result in a cell-lethal phenotype that also stimulates overgrowth of surrounding wild-type tissue.

              (Pfleger et al., 2007)

              Expression is enriched in embryonic gonads.

              (Shigenobu et al., 2006)

              dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

              (Kiger et al., 2003)

              Uba1 is required for axon pruning during metamorphosis.

              (Watts et al., 2003)

              Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

              (Roch et al., 1998)
              Origin and Etymology
              Discoverer
              Etymology
              Identification
              External Crossreferences and Linkouts ( 63 )
              Sequence Crossreferences
              NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
              GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
              GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
              RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
              UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
              Other crossreferences
              BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
              Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
              Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
              Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
              Flygut - An atlas of the Drosophila adult midgut
              GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
              iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
              InterPro - A database of protein families, domains and functional sites
              KEGG Genes - Molecular building blocks of life in the genomic space.
              modMine - A data warehouse for the modENCODE project
              Linkouts
              BioGRID - A database of protein and genetic interactions.
              DPiM - Drosophila Protein interaction map
              DroID - A comprehensive database of gene and protein interactions.
              DRSC - Results frm RNAi screens
              FLIGHT - Cell culture data for RNAi and other high-throughput technologies
              FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
              FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
              FlyMine - An integrated database for Drosophila genomics
              Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
              InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
              KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
              MIST (genetic) - An integrated Molecular Interaction Database
              MIST (protein-protein) - An integrated Molecular Interaction Database
              Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
              Synonyms and Secondary IDs (21)
              Reported As
              Symbol Synonym
              2/31
              (Roch et al., 1998)
              CG1782
              (Kolkhof et al., 2017, Çiçek et al., 2016, Christis and Munro, 2012, Ni et al., 2011.7.19, Perkins et al., 2009, Perkins et al., 2009.1.15, Puri et al., 2008, Nakahara et al., 2005, Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001)
              DmUba1
              (Shih et al., 2002)
              E1
              (Liu and Pfleger, 2013, Barry et al., 2011, Bergmann, 2010, Yan et al., 2009, Yan et al., 2008, Canning and Finnegan, 1999)
              FBtr0088499
              (Li et al., 2012)
              UBA-1
              (Kurshakova et al., 2019, Yadav and Srikrishna, 2019, Zur Lage, 1997.12.15)
              UBA1
              (Bergmann, 2010)
              Uba
              (Meltzer et al., 2019)
              Uba 1
              (Kraut et al., 2001, Kraut et al., 2001)
              Uba1
              (Levis, 2018.8.30, Ugrankar et al., 2018, Transgenic RNAi Project members, 2017-, Vazquez-Pianzola et al., 2017, Ashton-Beaucage et al., 2016, Kockel et al., 2016, Yadav et al., 2016, Yaniv and Schuldiner, 2016, Dent et al., 2015, Kanoh et al., 2015, Ugrankar et al., 2015, Chen et al., 2014, Colin et al., 2014, Lee et al., 2014, Mukherjee et al., 2014, Beckett et al., 2013, Chang et al., 2013, Kwon et al., 2013, Liu and Pfleger, 2013, Smibert et al., 2013, Li et al., 2012, Du et al., 2011, Franco et al., 2011, Lee et al., 2011, Kim et al., 2010, Saj et al., 2010, Sandu et al., 2010, Wasbrough et al., 2010, Carbone et al., 2009, Tan et al., 2009, Xu et al., 2009, Yan et al., 2009, FlyBase Curators, 2008-, Hoopfer et al., 2008, Lee et al., 2008, Schuldiner et al., 2008, Blard et al., 2007, Latouche et al., 2007, Pfleger et al., 2007, Shigenobu et al., 2006, Watts et al., 2003, Huet et al., 2002)
              l(2)03405
              (Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)
              l(2)05642
              (BDGP Project Members, 1994-1999)
              l(2)s3484
              uba-1
              (Wing et al., 2002)
              uba1
              (Jia et al., 2015, Wong et al., 2013, Mudiganti et al., 2010, Zhang et al., 2010, Lee et al., 2008, Simonsen et al., 2007, Staudt et al., 2005)
              Name Synonyms
              E1
              (Murphey and Godenschwege, 2002)
              E1 ligase
              (Martin et al., 2014)
              Ubiquitin Activating Enzyme
              (Liu and Pfleger, 2013, Yan et al., 2008)
              Ubiquitin activating enzyme 1
              (Blard et al., 2007, Tadros et al., 2007)
              ubiquitin activating enzyme
              (Barry et al., 2011)
              ubiquitin activating enzyme 1
              (Beller et al., 2008)
              ubiquitin-activating enzyme (E1)
              (Speese et al., 2003)
              Secondary FlyBase IDs
              • FBgn0010538
              • FBgn0010605
              • FBgn0010688
              Datasets (0)
              Study focus (0)
              Experimental Role
              Project
              Project Type
              Title
              References (139)