FlyBase Gene Report: Dmel\Uba1

General Information

Symbol

Dmel\Uba1

Species

D. melanogaster

Name

Ubiquitin activating enzyme 1

Annotation Symbol

CG1782

Feature Type

protein_coding_gene

FlyBase ID

FBgn0023143

Gene Model Status

Current

Stock Availability

21 publicly available

Gene Snapshot

Ubiquitin activating enzyme 1 (Uba1) encodes an E1 enzyme involved in protein ubiquitylation. It contributes to multiple processes including regulation of Ras-ERK signaling, autophagy, apoptosis and tissue growth. [Date last reviewed: 2019-07-11]

Other Summaries

Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

E1, UBA-1, l(2)03405, Uba 1, l(2)05642

Key Links

Genomic Location

Cytogenetic map

46A1-46A1

Sequence location

2R:9,688,289..9,693,328 [-]

Recombination map

2-61

RefSeq locus

NT_033778 REGION:9688289..9693328

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (20 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables ubiquitin activating enzyme activity

inferred from direct assay

(Chang et al., 2013)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables ubiquitin activating enzyme activity

inferred from biological aspect of ancestor with PANTHER:PTN000102404

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (15 terms)

Terms Based on Experimental Evidence (12 terms)

CV Term

Evidence

References

involved_in autophagy

inferred from mutant phenotype

(Chang et al., 2013)

involved_in glucose homeostasis

inferred from mutant phenotype

(Ugrankar et al., 2015)

involved_in larval midgut cell programmed cell death

inferred from mutant phenotype

(Chang et al., 2013)

involved_in lipid storage

inferred from direct assay

(Beller et al., 2008)

involved_in mushroom body development

inferred from mutant phenotype

(Schuldiner et al., 2008)

involved_in neuron remodeling

inferred from mutant phenotype

(Watts et al., 2003)

involved_in ovarian follicle cell development

inferred from mutant phenotype

(Jia et al., 2015)

involved_in protein polyubiquitination

inferred from mutant phenotype

(Lee et al., 2014)

involved_in protein ubiquitination

inferred from direct assay

(Chang et al., 2013)

inferred from mutant phenotype

(Yan et al., 2009, Lee et al., 2008)

involved_in regulation of growth

inferred from mutant phenotype

(Lee et al., 2008)

involved_in regulation of programmed cell death

inferred from mutant phenotype

(Lee et al., 2008)

inferred from genetic interaction with FLYBASE:hid; FB:FBgn0003997

(Lee et al., 2008)

involved_in regulation of Ras protein signal transduction

inferred from mutant phenotype

(Yan et al., 2009)

Terms Based on Predictions or Assertions (4 terms)

CV Term

Evidence

References

involved_in cellular response to DNA damage stimulus

inferred from biological aspect of ancestor with PANTHER:PTN000102404

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein modification by small protein conjugation

inferred from biological aspect of ancestor with PANTHER:PTN000102039

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in protein ubiquitination

inferred from biological aspect of ancestor with PANTHER:PTN000102404

(assigned by GO_Central )

(Gaudet et al., 2011)

involved_in ubiquitin-dependent protein catabolic process

inferred from biological aspect of ancestor with PANTHER:PTN000102404

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

located_in dendrite

inferred from mutant phenotype

(Kuo et al., 2005)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

located_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000102040

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000102404

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

UBIQUITIN ACTIVATING ENZYMES

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

UBIQUITIN ACTIVATING ENZYMES -: Ubiquitin activating enzymes are 'canonical' E1 enzymes that activate ubiquitin for subsequent steps along the ubiquitin conjugation cascade. They perform three distinct chemical reactions: first, they bind ATP, magnesium and ubiquitin to catalyze formation of a high-energy acyl adenylate intermediate with subsequent release of pyrophosphate; second, an E1 catalytic cysteine attacks the ubiquitin-adenylate to catalyze formation of a high-energy thioester bond between the E1 and ubiquitin and release of the AMP product; finally, the E1-ubiquitin catalyzes transthioesterification to an E2 to form a high energy thioester-linked E2-ubiquitin product. (Adapted from PMID:28234446.)

Summary (Interactive Fly)

Ubiquitin-activating enzyme, E1, activates and transfers ubiquitin to ubiquitin-conjugating enzymes, tumor suppressor

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Uba1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.42

Gene model reviewed during 5.49

Low-frequency RNA-Seq exon junction(s) not annotated.

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Uba1-RA

FBtr0088499

NM_057962

4343

1191

Uba1-RC

FBtr0339517

NM_001273902

4343

1008

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

Uba1-PA

FBpp0087583

130.8

1191

4.93

NP_477310

AAF58910

Uba1-PC

FBpp0308600

111.8

1008

4.91

NP_001260831

AGB93364

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Uba1 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Uba1_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

prepupal stage P1

gamma Kenyon cell

(Hoopfer et al., 2008)

late third instar larval stage

gamma Kenyon cell | faint

(Hoopfer et al., 2008)

embryonic stage 1 -- 3

organism | ubiquitous

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

dorsal ectoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

antennal primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

central brain primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

visual primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

dorsal head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

lateral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

ventral head epidermis primordium

Comment: reported as procephalic ectoderm primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic hindgut

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

embryonic/larval nervous system

(Fisher et al., 2012)

gonad

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 5 of adulthood & male -- day 7 of adulthood & male

spermatozoon

(Wasbrough et al., 2010)

adult stage

adult head

• membrane

(Aradska et al., 2015)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in dendrite

inferred from mutant phenotype

(Kuo et al., 2005)

located_in plasma membrane

inferred from high throughput direct assay

(Tan et al., 2009)

Expression Deduced from Reporters

Reporter: P{ST.lexA::HG}Uba1^DT-5

Stage

Tissue/Position (including subcellular localization)

Reference

wandering third instar larval stage

embryonic/larval peripheral nervous system

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval salivary gland

(Bloomington Drosophila Stock Center, 2019)

embryonic/larval epidermis

(Bloomington Drosophila Stock Center, 2019)

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Uba1 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Uba1

View exonic expression by tissue for Dmel\Uba1

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023143

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0023143

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1782-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1782

Flygut - An atlas of the Drosophila adult midgut

FBgn0023143

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Uba1

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 43 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 11 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Uba1

Transgenic constructs containing regulatory region of Uba1

Deletions and Duplications ( 2 )

Not disrupted in

Df(2R)B5

(BDGP Project Members, 1994-1999)

Df(2R)X3

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

FlyBase_internaltransgene_features:cell lethal:cell lethal | somatic clone | heat sensitive

lethal

lethal (with Uba1⁰³⁴⁰⁵)

lethal (with Uba1^D6)

lethal (with Uba1^H33)

lethal (with Uba1^H42)

lethal (with Uba1^s3484)

lethal (with Uba1^UY3010)

Uba1^s3484

lethal | recessive

lethal - all die before end of P-stage | recessive

Uba1^B2

lethal - all die before end of pupal stage

Uba1^B1

partially lethal - majority die

Uba1⁰⁵⁶⁴²

viable, with Scer\GAL4^da.G32

Uba1^UY3010

viable, with Scer\GAL4^ptc.PU

Uba1^UY3010

viable, with Scer\GAL4^vg.PM

Uba1^UY3010

viable (with Uba1^s3484), with Scer\GAL4^da.G32

Uba1^UY3010

viable (with Uba1^UY3010), with Scer\GAL4^da.G32

Uba1^s3484

Sterility

Allele

sterile

Uba1^B1

sterile (with Uba1^B1)

Uba1^B2

sterile (with Uba1^B2)

Uba1^B1

Other Phenotypes

Allele

chemical sensitive, with Scer\GAL4^elav.PU

Uba1^dsRNA.UAS

decreased cell death | pupal stage | somatic clone

Uba1^D6

decreased occurrence of cell division | larval stage | somatic clone

Uba1^D6

exocytosis defective | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Uba1^JF01977

increased cell death | pupal stage | somatic clone | heat sensitive

Uba1^D6

increased cell number | cell non-autonomous | somatic clone

Uba1^A1

increased occurrence of cell division | cell non-autonomous | larval stage | somatic clone

Uba1^D6

increased occurrence of cell division | larval stage (with Uba1^B1)

Uba1^B2

increased occurrence of cell division | larval stage (with Uba1^B2)

Uba1^B1

locomotor behavior defective

Uba1^B1

locomotor behavior defective (with Uba1^B1)

Uba1^B2

locomotor behavior defective (with Uba1^B2)

Uba1^B1

neuroanatomy defective | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

neuroanatomy defective | larval stage, with Scer\GAL4^elav-C155

Uba1^EP2609

neuroanatomy defective | pupal stage

Uba1^s3484

neuroanatomy defective | somatic clone

short lived, with Scer\GAL4^elav.PU

Uba1^dsRNA.UAS

short lived | dominant

size defective | adult stage

Uba1^D6

some die during pupal stage

Uba1^B1

visible (with Uba1^B1)

Uba1^B2

visible (with Uba1^B2)

Uba1^B1

visible | recessive

Uba1^B1

visible | somatic clone

visible | somatic clone | heat sensitive

Phenotype manifest in

Allele

adult mushroom body | somatic clone

Uba1^LL03617

anterior fascicle & synapse, with Scer\GAL4^elav-C155

Uba1^EP2609

anterior fascicle & synapse | supernumerary, with Scer\GAL4^elav-C155

Uba1^EP2609

axon | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

axon | adult stage | somatic clone

Uba1^LL03617

dendrite | somatic clone

Uba1^s3484

dendritic arborizing neuron | P-stage

Uba1^s3484

dopaminergic neuron | chemical conditional, with Scer\GAL4^elav.PU

Uba1^dsRNA.UAS

dorsocentral bristle

Uba1^B1

embryonic/larval fat body | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Uba1^JF01977

extracellular matrix | third instar larval stage, with Scer\GAL4^{SPARC-MI00329-GAL4}

Uba1^JF01977

eye | cell non-autonomous | somatic clone

eye | somatic clone

eye | somatic clone | heat sensitive

eye-antennal disc | somatic clone | third instar larval stage | heat sensitive

eye disc | cell non-autonomous | somatic clone

Uba1^A1

eye disc | pupal stage | somatic clone

Uba1^D6

eye disc | somatic clone

Uba1^s3484

eye disc posterior to the morphogenetic furrow

eye disc posterior to the morphogenetic furrow (with Uba1^B1)

Uba1^B2

eye disc posterior to the morphogenetic furrow (with Uba1^B2)

Uba1^B1

gamma Kenyon cell | adult stage, with Scer\GAL4^GMR71G10, Spyo\Cas9^{UAS.Tag:NLS(Unk)}

humeral disc

Uba1^B1

interommatidial cell | pupal stage | somatic clone

Uba1^H33

intersegmental nerve, with Scer\GAL4^elav-C155

Uba1^EP2609

leg

Uba1^B1

macrochaeta

Uba1^L46A1

mushroom body & axon | somatic clone

Uba1^s3484

neuroblast | somatic clone

Uba1^s3484

proboscis

Uba1^B1

retina | P-stage | somatic clone

Uba1^B2

sternopleural bristle | supernumerary

Uba1^B1

wing

Uba1^B1

wing vein

Uba1^B1

wing vein (with Uba1^B1)

Uba1^B2

wing vein (with Uba1^B2)

Uba1^B1

wing vein | ectopic

Uba1^B1

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (10)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\UBA1

13 of 15

Yes

Hsap\UBA7

7 of 15

Yes

Hsap\UBA6

4 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (11)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Uba1

13 of 15

Yes

Mmus\Uba1y

11 of 15

Yes

Mmus\Uba7

7 of 15

Yes

Mmus\Uba6

3 of 15

Yes

2 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Rattus norvegicus (Norway rat) (13)

Rnor\Uba1

12 of 13

Yes

Rnor\Uba1y

6 of 13

Yes

Rnor\Uba6

4 of 13

Yes

Rnor\Uba7

4 of 13

Yes

Rnor\LOC103694556

3 of 13

Yes

Rnor\LOC103694557

3 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Xenopus tropicalis (Western clawed frog) (9)

Xtro\LOC100495145

5 of 12

Yes

Xtro\uba6

3 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Xtro\uba2

1 of 12

Xtro\uba3

1 of 12

Xtro\uba5

1 of 12

Danio rerio (Zebrafish) (8)

Drer\uba1

13 of 15

Yes

Drer\uba7

7 of 15

Yes

Drer\si:dkey-82j4.2

3 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (6)

Cele\uba-1

12 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Arabidopsis thaliana (thale-cress) (2)

Atha\UBA1

8 of 9

Yes

Atha\UBA 2

8 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (7)

Scer\UBA1

13 of 15

Yes

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

1 of 15

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\ptr3

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG0919015Y )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA14681

Drosophila persimilis

Dper\GL17328

Drosophila willistoni

Dwil\GK15620

Drosophila virilis

Dvir\GJ20835

Drosophila mojavensis

Dmoj\GI21231

Drosophila grimshawi

Dgri\GH23328

Drosophila grimshawi

Dgri\GH22854

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500YT )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17019149

Musca domestica

House fly

Mdoa\17019150

Glossina morsitans

Tsetse fly

Gmor\GMOY001369

Mayetiola destructor

Hessian fly

Mdes\Mdes009753

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL000758

Anopheles darlingi

American malaria mosquito

Adar\ADAC008378

Anopheles gambiae

Malaria mosquito

Agam\AGAP011872

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016556

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ001423

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00RX )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA009911

Danaus plexippus

Monarch butterfly

Dple\DPOGS207441-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL012328

Apis florea

Little honeybee

Aflo\2921393092

Apis mellifera

Western honey bee

Amel\Uba1

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP13938

Bombus terrestris

Buff-tailed bumblebee

Bter\15215337

Linepithema humile

Argentine ant

Lhum\LH12261

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:000bdb

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG024134

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_10832

Tribolium castaneum

Red flour beetle

Tcas\TC031473

Pediculus humanus

Human body louse

Phum\PHUM354890

Rhodnius prolixus

Kissing bug

Rpro\RPRC003935

Cimex lectularius

Bed bug

Clec\CLEC025155

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI006009

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08188

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00QH )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR001087

Ixodes scapularis

Black-legged tick

Isca\ISCW019123

Stegodyphus mimosarum

African social velvet spider

Smim\KFM79356.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur12g01800

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_186898

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0130 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Uba6-2

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Uba6

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Uba1

Ciona intestinalis

Vase tunicate

Cint\LOC100176463

Gallus gallus

Domestic chicken

Ggal\UBA6

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

3 of 10

1 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 1 )

Human Ortholog

Disease

Evidence

References

UBA1; ubiquitin like modifier activating enzyme 1

model of X-linked spinal muscular atrophy 2

IEA

(FlyBase, 2019-)

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

Uba1^UY3010

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN7^{102Q.T.UAS.Tag:NLS(SV40-largeT)}

(Latouche et al., 2007)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

UBA1; ubiquitin like modifier activating enzyme 1

13 of 15

314370

X-linked spinal muscular atrophy 2

UBA7; ubiquitin like modifier activating enzyme 7

7 of 15

191325

UBA6; ubiquitin like modifier activating enzyme 6

4 of 15

611361

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Uba1 - ben

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Uba1

suppressible

Diap1

(Pfleger et al., 2007)

Ras85D|Uba1

suppressible

egr

(Yan et al., 2009)

Uba1

suppressible

(Lee et al., 2008)

Uba1

suppressible

Ras85D

(Liu and Pfleger, 2013, Yan et al., 2009)

Uba1

suppressible

Stat92E

(Lee et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bchs

enhanceable

Uba1

(Simonsen et al., 2007)

enhanceable

(Kim et al., 2010)

suppressible

(Colin et al., 2014)

egr

suppressible

Uba1

(Pfleger et al., 2007)

grim::rpr

enhanceable

Uba1

(Wing et al., 2002)

grim

suppressible

Uba1

(Pfleger et al., 2007)

hid

suppressible

Uba1

(Christiansen et al., 2013, Lee et al., 2008, Pfleger et al., 2007)

hpo

enhanceable

Uba1

(Pfleger et al., 2007)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61829

DroID - A comprehensive database of gene and protein interactions.

FBgn0023143

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35998

MIST (genetic) - An integrated Molecular Interaction Database

Uba1

MIST (protein-protein) - An integrated Molecular Interaction Database

Uba1

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ubiquitin mediated proteolysis

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-61

Cytogenetic map

46A1-46A1

Sequence location

2R:9,688,289..9,693,328 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

46A1-46A1

Limits computationally determined from genome sequence between P{PZ}Mmp2⁰²³⁵³&P{PZ}Uba1⁰⁵⁶⁴² and P{lacW}l(2)k09221^k09221&P{PZ}dap⁰⁴⁴⁵⁴

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

46A-46A

(Zur Lage, 1997.12.15)

46A1-46A2

(determined by in situ hybridisation)

(Spradling et al., 1999)

46A1-46A2

46A2--3 46A2--4 46A3--4

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

2-61

(FlyBase, 2016)

2-59.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (21)

Bloomington

25957

y¹ v¹; P{TRiP.JF01977}attP2

76066

y¹ v¹; P{TRiP.HMS05878}attP2

11413

cn¹ P{PZ}Uba1⁰⁵⁶⁴²/CyO; ry⁵⁰⁶

82783

y¹ v¹; M{WKO.1-D11}ZH-86Fb/TM6B, Tb¹

Kyoto

104061

w^*; P{GawB}Uba1^NP2006 / CyO

113530

w^*; P{GawB}Uba1^NP4810 / CyO

102128

y¹ w^67c23 ; P{lacW}Uba1^k00203 / CyO

102135

y¹ w^67c23 ; P{lacW}Uba1^k00231 / CyO

102184

y¹ w^67c23 ; P{lacW}Uba1^k01016 / CyO

102550

y¹ w^67c23 ; P{lacW}Uba1^k07246 / CyO

102570

y¹ w^67c23 ; P{lacW}Uba1^k07517 / CyO

102829

y¹ w^67c23 ; P{lacW}Uba1^k12102 / CyO

More stocks available...

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (188)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

GH24511

Other clones

DPiM_GH24511

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0023143

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0023143

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35998

Antibody Information

Laboratory Generated Antibodies

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Uba1 CG1782

(Evangelista, 1999.11)

Source for database merge of

Source for merge of: Uba1 l(2)03405

(Spradling et al., 1999)

Source for merge of: Uba1 l(2)05642

(Misra, 2000.11.18)

Additional comments

Other Comments

Uba1 controls apoptosis autonomously and tissue growth non-autonomously.

(Lee et al., 2008)

Uba1 mutations that appear to be hypomorphic elicit a mild overrepresentation of mutant tissue in a mosaic eye, while Uba1 mutations that represent strong or null alleles result in a cell-lethal phenotype that also stimulates overgrowth of surrounding wild-type tissue.

(Pfleger et al., 2007)

Expression is enriched in embryonic gonads.

(Shigenobu et al., 2006)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Uba1 is required for axon pruning during metamorphosis.

(Watts et al., 2003)

Mutants isolated in a screen of the second chromosome identifying genes affecting disc morphology.

(Roch et al., 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 63 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35998

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023143

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0023143

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Uba1_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1782

Flygut - An atlas of the Drosophila adult midgut

FBgn0023143

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35998

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031473

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1782

modMine - A data warehouse for the modENCODE project

FBgn0023143

Linkouts

BioGRID - A database of protein and genetic interactions.

61829

DPiM - Drosophila Protein interaction map

FBgn0023143

DroID - A comprehensive database of gene and protein interactions.

FBgn0023143

DRSC - Results frm RNAi screens

FBgn0023143

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0023143

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1782-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0023143

FlyMine - An integrated database for Drosophila genomics

FBgn0023143

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35998

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

Ubiquitin mediated proteolysis

MIST (genetic) - An integrated Molecular Interaction Database

Uba1

MIST (protein-protein) - An integrated Molecular Interaction Database

Uba1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

2/31

(Roch et al., 1998)

CG1782

(Kolkhof et al., 2017, Çiçek et al., 2016, Christis and Munro, 2012, Ni et al., 2011.7.19, Perkins et al., 2009, Perkins et al., 2009.1.15, Puri et al., 2008, Nakahara et al., 2005, Norga et al., 2003, Kraut et al., 2001, Kraut et al., 2001)

DmUba1

(Shih et al., 2002)

(Liu and Pfleger, 2013, Barry et al., 2011, Bergmann, 2010, Yan et al., 2009, Yan et al., 2008, Canning and Finnegan, 1999)

FBtr0088499

(Li et al., 2012)

UBA-1

(Kurshakova et al., 2019, Yadav and Srikrishna, 2019, Zur Lage, 1997.12.15)

UBA1

(Bergmann, 2010)

Uba

(Meltzer et al., 2019)

Uba 1

(Kraut et al., 2001, Kraut et al., 2001)

Uba1

(Xu et al., 2020, Levis, 2018.8.30, Ugrankar et al., 2018, Transgenic RNAi Project members, 2017-, Vazquez-Pianzola et al., 2017, Ashton-Beaucage et al., 2016, Kockel et al., 2016, Yadav et al., 2016, Yaniv and Schuldiner, 2016, Dent et al., 2015, Kanoh et al., 2015, Ugrankar et al., 2015, Chen et al., 2014, Colin et al., 2014, Lee et al., 2014, Mukherjee et al., 2014, Beckett et al., 2013, Chang et al., 2013, Kwon et al., 2013, Liu and Pfleger, 2013, Smibert et al., 2013, Li et al., 2012, Du et al., 2011, Franco et al., 2011, Lee et al., 2011, Kim et al., 2010, Saj et al., 2010, Sandu et al., 2010, Wasbrough et al., 2010, Carbone et al., 2009, Tan et al., 2009, Xu et al., 2009, Yan et al., 2009, FlyBase Curators, 2008-, Hoopfer et al., 2008, Lee et al., 2008, Schuldiner et al., 2008, Blard et al., 2007, Latouche et al., 2007, Pfleger et al., 2007, Shigenobu et al., 2006, Watts et al., 2003, Huet et al., 2002)

l(2)03405

(Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

l(2)05642

(BDGP Project Members, 1994-1999)

l(2)s3484

uba-1

(Wing et al., 2002)

uba1

(Jia et al., 2015, Wong et al., 2013, Mudiganti et al., 2010, Zhang et al., 2010, Lee et al., 2008, Simonsen et al., 2007, Staudt et al., 2005)

Name Synonyms

(Murphey and Godenschwege, 2002)

E1 ligase

(Martin et al., 2014)

Ubiquitin Activating Enzyme

(Liu and Pfleger, 2013, Yan et al., 2008)

Ubiquitin activating enzyme 1

(Blard et al., 2007, Tadros et al., 2007)

ubiquitin activating enzyme

(Barry et al., 2011)

ubiquitin activating enzyme 1

(Beller et al., 2008)

ubiquitin-activating enzyme (E1)

(Speese et al., 2003)

Secondary FlyBase IDs

FBgn0010538
FBgn0010605
FBgn0010688

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (143)

The following external sites may use different assemblies or annotations than FlyBase.

Report Sections