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Gene Dmel\SNF1A

General Information
Symbol	Dmel\SNF1A	Species	D. melanogaster
Name	SNF1A/AMP-activated protein kinase	Annotation symbol	CG3051
Feature type	protein_coding_gene	FlyBase ID	FBgn0023169
Gene Model Status	Current	Stock availability	13 publicly available
Also Known As	AMPK, AMPKα, EG:132E8.2, dAMPKα
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	2A1-2A1	Sequence location	X:1,269,569..1,272,929 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene SNF1A/AMP-activated protein kinase is referred to in FlyBase by the symbol Dmel\SNF1A (CG3051, FBgn0023169). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: AMP-activated protein kinase activity. There is experimental evidence for 12 unique biological process terms, many of which group under: biological regulation; cellular component organization or biogenesis; response to nutrient levels; response to starvation; localization; primary metabolic process; cellular process; macromolecule modification; energy taxis; cell cycle. 31 alleles are reported. The phenotypes of these alleles are annotated with: organ system; multicellular structure; anatomical structure; organelle; portion of tissue; intracellular organelle part; embryonic/larval fat body; centrosome; organ system subdivision; embryonic/larval hypodermal muscle; spindle; intracellular non-membrane-bounded organelle; peripheral nervous system. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; Serine/threonine- / dual specificity protein kinase, catalytic domain; Serine/threonine-protein kinase, active site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, in stages of adults of both sexes. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult heart, larval fat body, larval salivary gland, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1629357_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of SNF1A. Gene sequence location is X:1269569..1272929.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 2A1-2A1   Limits computationally determined from genome sequence between P{EP}EP1542 and P{EP}futschEP1419
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\SNF1A for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) SNF1A-RA FBtr0070216  NM_057965   2708   582 SNF1A-RB FBtr0070217  NM_166880   2853   582 SNF1A-RC FBtr0070218  NM_206604   2739   582
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein SNF1A-PA   FBpp0070208   64.6   582   8.60     NP_477313   AAF45614 SNF1A-PB   FBpp0070209   64.6   582   8.60     NP_726730   AAN09043 SNF1A-PC   FBpp0089374   64.6   582   8.60     NP_996327   AAS65245
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA246384   AA391609   AA439586   AA696476   AA735437   AA803336   AE014298 AAF45614   AE014298 AAN09043   AE014298 AAS65245   AF020309 AAB71397   AF020310 AAB71398   AF181649 AAD55435   AI063199   AI063258   AI063443   AI064277   AI106896   AI107989   AI108354   AI108905   AI113879   AI133989   AI134993   AI135181   AI238006   AI259215   AI293124   AI387243   AI389124   AI402294   AI405821   AI543016   AI546570   BF505150   BG635456   BG638264   BI161488   BI161497   BI166329   BI171245   BI172645   BI228287   BI232308   BI235622   BI358328   BI372032   BI482896   BI564472   BI583888   BI586136   BI587994   BI590158   BI611758   BI613033   BI637005   BI637024   BI641462   BT016074 AAV36959   BT044168   BX042708   BX055084   BX064632   CO273471   CO285835   CO298340   CO300770   CO314774   CO317520   EC052916   EC053856   EC055258   EC060481   EC062465   EC064936   EC065457   EC080019   EC230377   EC238651   EC255171   EC264298   EL869724   EL869725   EL873356   EL873357   EL873468   EL873469   EL875527   EL875528   EL880720   EL883641   EV579799   GH941342   GH942767   GH943278   GH946388   GH946995   GH948927   GH951234   GH951472   GH951635   GH952513   GH954161   GH956404   GH962236   GH966100   GH966307   GH966367   GH968377   GH968488   GH969500   GH970308   GH971009   GH971217   GH975044   GH977026   GH978380   GH979716   GH981003   GH983677   GH983692   GH984162   GH984200
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O18645 Q7KK41 Q5U118
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Mirouse et al., 2007) nucleus inferred from direct assay (Pan and Hardie, 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, adult heart, larval fat body, larval salivary gland, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut    656 Larval Hindgut    796 Larval Malpighian Tubules    781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea    531.9 Larval Carcass    534.175 Adult Head    632.4 Adult Eye    962.9 Adult Brain    693.1 Adult Thoracic-Abdominal Ganglion    680.2 Adult Crop    687.6 Adult Midgut    636.4 Adult Hindgut    728 Adult Malpighian Tubules    992.9 Adult Fat Body    536.7 Adult Salivary Gland    1178.1 Adult Heart    479.7 Adult VirginFemale Spermatheca    631.6 Adult InseminatedFemale Spermatheca    599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland    564.3 Adult Carcass    820 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut  (656) Larval Hindgut  (796) Larval Malpighian Tubules  (781.6) Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea  531.9 Larval Carcass  534.175 Adult Head  (632.4) Adult Eye  (962.9) Adult Brain  (693.1) Adult Thoracic-Abdominal Ganglion  (680.2) Adult Crop  (687.6) Adult Midgut  (636.4) Adult Hindgut  (728) Adult Malpighian Tubules  (992.9) Adult Fat Body  536.7 Adult Salivary Gland  (1178.1) Adult Heart    479.7 Adult VirginFemale Spermatheca  (631.6) Adult InseminatedFemale Spermatheca  (599.2) Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland  (564.3) Adult Carcass  (820) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut    656 Larval Hindgut    796 Larval Malpighian Tubules    781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea    531.9 Larval Carcass    534.175 Adult Head    632.4 Adult Eye    962.9 Adult Brain    693.1 Adult Thoracic-Abdominal Ganglion    680.2 Adult Crop    687.6 Adult Midgut    636.4 Adult Hindgut    728 Adult Malpighian Tubules    992.9 Adult Fat Body    536.7 Adult Salivary Gland  (1178.1) Adult Heart    479.7 Adult VirginFemale Spermatheca    631.6 Adult InseminatedFemale Spermatheca    599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland    564.3 Adult Carcass    820 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut    656 Larval Hindgut    796 Larval Malpighian Tubules    781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea    531.9 Larval Carcass    534.175 Adult Head    632.4 Adult Eye    962.9 Adult Brain    693.1 Adult Thoracic-Abdominal Ganglion    680.2 Adult Crop    687.6 Adult Midgut    636.4 Adult Hindgut    728 Adult Malpighian Tubules    992.9 Adult Fat Body    536.7 Adult Salivary Gland    1178.1 Adult Heart    479.7 Adult VirginFemale Spermatheca    631.6 Adult InseminatedFemale Spermatheca    599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland    564.3 Adult Carcass    820 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut    656 Larval Hindgut    796 Larval Malpighian Tubules    781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea    531.9 Larval Carcass    534.175 Adult Head    632.4 Adult Eye    962.9 Adult Brain    693.1 Adult Thoracic-Abdominal Ganglion    680.2 Adult Crop    687.6 Adult Midgut    636.4 Adult Hindgut    728 Adult Malpighian Tubules    992.9 Adult Fat Body    536.7 Adult Salivary Gland    1178.1 Adult Heart    479.7 Adult VirginFemale Spermatheca    631.6 Adult InseminatedFemale Spermatheca    599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland    564.3 Adult Carcass    820 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut  656 Larval Hindgut  796 Larval Malpighian Tubules  781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea  531.9 Larval Carcass  534.175 Adult Head  632.4 Adult Eye  (962.9) Adult Brain  693.1 Adult Thoracic-Abdominal Ganglion  680.2 Adult Crop  687.6 Adult Midgut  636.4 Adult Hindgut  728 Adult Malpighian Tubules  (992.9) Adult Fat Body  536.7 Adult Salivary Gland  (1178.1) Adult Heart    479.7 Adult VirginFemale Spermatheca  631.6 Adult InseminatedFemale Spermatheca  599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland  564.3 Adult Carcass  (820) Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut    656 Larval Hindgut    796 Larval Malpighian Tubules    781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea    531.9 Larval Carcass    534.175 Adult Head    632.4 Adult Eye    962.9 Adult Brain    693.1 Adult Thoracic-Abdominal Ganglion    680.2 Adult Crop    687.6 Adult Midgut    636.4 Adult Hindgut    728 Adult Malpighian Tubules    992.9 Adult Fat Body    536.7 Adult Salivary Gland  1178.1 Adult Heart    479.7 Adult VirginFemale Spermatheca    631.6 Adult InseminatedFemale Spermatheca    599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland    564.3 Adult Carcass    820 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    471.475 Larval Midgut    656 Larval Hindgut    796 Larval Malpighian Tubules    781.6 Larval Fat Body    428.4 Larval Salivary Gland    474.3 Larval Trachea    531.9 Larval Carcass    534.175 Adult Head    632.4 Adult Eye    962.9 Adult Brain    693.1 Adult Thoracic-Abdominal Ganglion    680.2 Adult Crop    687.6 Adult Midgut    636.4 Adult Hindgut    728 Adult Malpighian Tubules    992.9 Adult Fat Body    536.7 Adult Salivary Gland    1178.1 Adult Heart    479.7 Adult VirginFemale Spermatheca    631.6 Adult InseminatedFemale Spermatheca    599.2 Adult Ovary    358.1 Adult Testis    197 Adult Male Accessory Gland    564.3 Adult Carcass    820 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    632.4   NA  Eye    962.9   NA  Brain    693.1   471.475  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    680.2   NA  Crop    687.6   656  Midgut    636.4   796  Hindgut    728   781.6  Malpighian Tubules    992.9   428.4  Fat Body    536.7   474.3  Salivary Gland    1178.1   NA  Heart    479.7   531.9  Trachea    NA   NA  VirginFemale Spermatheca    631.6   NA  InseminatedFemale Spermatheca    599.2   NA  Ovary    358.1   NA  Testis    197   NA  Male Accessory Gland    564.3   534.175  Carcass    820 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female    29 head, virgin 4-day female    32 head, virgin 20-day female    42 head, mated 1-day female    27 head, mated 4-day female    25 head, mated 20-day female    33 head, mated 1-day male    43 head, mated 4-day male    36 head, mated 20-day male    42 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    34 digestive system, 1-day adult    32 digestive system, 4-day adult    25 digestive system, 20-day adult    37 fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8    32 carcass, larvae L3 wandering    23 carcass, 1-day adult    29 carcass, 4-day adult    25 carcass, 20-day adult    29 ovary, virgin 4-day female    36 ovary, mated 4-day female    30 testis, mated 4-day male    33 accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (31) central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female  (29) head, virgin 4-day female  (32) head, virgin 20-day female  (42) head, mated 1-day female  (27) head, mated 4-day female    25 head, mated 20-day female  (33) head, mated 1-day male  (43) head, mated 4-day male  (36) head, mated 20-day male  (42) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering  (34) digestive system, 1-day adult  (32) digestive system, 4-day adult    25 digestive system, 20-day adult  (37) fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8  (32) carcass, larvae L3 wandering    23 carcass, 1-day adult  (29) carcass, 4-day adult    25 carcass, 20-day adult  (29) ovary, virgin 4-day female  (36) ovary, mated 4-day female  (30) testis, mated 4-day male  (33) accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female    29 head, virgin 4-day female    32 head, virgin 20-day female    42 head, mated 1-day female    27 head, mated 4-day female    25 head, mated 20-day female    33 head, mated 1-day male    43 head, mated 4-day male    36 head, mated 20-day male    42 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    34 digestive system, 1-day adult    32 digestive system, 4-day adult    25 digestive system, 20-day adult    37 fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8    32 carcass, larvae L3 wandering    23 carcass, 1-day adult    29 carcass, 4-day adult    25 carcass, 20-day adult    29 ovary, virgin 4-day female    36 ovary, mated 4-day female    30 testis, mated 4-day male    33 accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female    29 head, virgin 4-day female    32 head, virgin 20-day female    42 head, mated 1-day female    27 head, mated 4-day female    25 head, mated 20-day female    33 head, mated 1-day male    43 head, mated 4-day male    36 head, mated 20-day male    42 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    34 digestive system, 1-day adult    32 digestive system, 4-day adult    25 digestive system, 20-day adult    37 fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8    32 carcass, larvae L3 wandering    23 carcass, 1-day adult    29 carcass, 4-day adult    25 carcass, 20-day adult    29 ovary, virgin 4-day female    36 ovary, mated 4-day female    30 testis, mated 4-day male    33 accessory gland, mated 4-day male    19 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female    29 head, virgin 4-day female    32 head, virgin 20-day female    42 head, mated 1-day female    27 head, mated 4-day female    25 head, mated 20-day female    33 head, mated 1-day male    43 head, mated 4-day male    36 head, mated 20-day male    42 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    34 digestive system, 1-day adult    32 digestive system, 4-day adult    25 digestive system, 20-day adult    37 fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8    32 carcass, larvae L3 wandering    23 carcass, 1-day adult    29 carcass, 4-day adult    25 carcass, 20-day adult    29 ovary, virgin 4-day female    36 ovary, mated 4-day female    30 testis, mated 4-day male    33 accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female  29 head, virgin 4-day female  32 head, virgin 20-day female  (42) head, mated 1-day female  27 head, mated 4-day female    25 head, mated 20-day female  (33) head, mated 1-day male  (43) head, mated 4-day male  (36) head, mated 20-day male  (42) salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering  (34) digestive system, 1-day adult  32 digestive system, 4-day adult    25 digestive system, 20-day adult  (37) fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8  32 carcass, larvae L3 wandering    23 carcass, 1-day adult  29 carcass, 4-day adult    25 carcass, 20-day adult  29 ovary, virgin 4-day female  (36) ovary, mated 4-day female  30 testis, mated 4-day male  (33) accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female    29 head, virgin 4-day female    32 head, virgin 20-day female    42 head, mated 1-day female    27 head, mated 4-day female    25 head, mated 20-day female    33 head, mated 1-day male    43 head, mated 4-day male    36 head, mated 20-day male    42 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    34 digestive system, 1-day adult    32 digestive system, 4-day adult    25 digestive system, 20-day adult    37 fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8    32 carcass, larvae L3 wandering    23 carcass, 1-day adult    29 carcass, 4-day adult    25 carcass, 20-day adult    29 ovary, virgin 4-day female    36 ovary, mated 4-day female    30 testis, mated 4-day male    33 accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    31 central nervous system, larvae L3    21 central nervous system, pupae P8    22 head, virgin 1-day female    29 head, virgin 4-day female    32 head, virgin 20-day female    42 head, mated 1-day female    27 head, mated 4-day female    25 head, mated 20-day female    33 head, mated 1-day male    43 head, mated 4-day male    36 head, mated 20-day male    42 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    10 digestive system, larvae L3 wandering    34 digestive system, 1-day adult    32 digestive system, 4-day adult    25 digestive system, 20-day adult    37 fat body, larvae L3 wandering    12 fat body, white prepupae    16 fat body, pupae P8    32 carcass, larvae L3 wandering    23 carcass, 1-day adult    29 carcass, 4-day adult    25 carcass, 20-day adult    29 ovary, virgin 4-day female    36 ovary, mated 4-day female    30 testis, mated 4-day male    33 accessory gland, mated 4-day male    19 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderately high expression. Peak expression observed at stages throughout embryogenesis, in stages of adults of both sexes. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    71 embryo 02-04hr    38 embryo 04-06hr    35 embryo 06-08hr    49 embryo 08-10hr    47 embryo 10-12hr    61 embryo 12-14hr    42 embryo 14-16hr    54 embryo 16-18hr    45 embryo 18-20hr    61 embryo 20-22hr    58 embryo 22-24hr    56 larva L1    44 larva L2    34 larva L3 12hr old    36 larva L3 puffstage 1-2    40 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    45 white prepupae new    38 white prepupae 12hr    44 white prepupae 24hr    45 pupae 2d postWPP    39 pupae 3d postWPP    39 pupae 4d postWPP    47 adult male 01day    62 adult male 05day    71 adult male 30day    62 adult female 01day    67 adult female 05day    54 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (71) embryo 02-04hr  (38) embryo 04-06hr  (35) embryo 06-08hr  (49) embryo 08-10hr  (47) embryo 10-12hr  (61) embryo 12-14hr  (42) embryo 14-16hr  (54) embryo 16-18hr  (45) embryo 18-20hr  (61) embryo 20-22hr  (58) embryo 22-24hr  (56) larva L1  (44) larva L2  (34) larva L3 12hr old  (36) larva L3 puffstage 1-2  (40) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (45) white prepupae new  (38) white prepupae 12hr  (44) white prepupae 24hr  (45) pupae 2d postWPP  (39) pupae 3d postWPP  (39) pupae 4d postWPP  (47) adult male 01day  (62) adult male 05day  (71) adult male 30day  (62) adult female 01day  (67) adult female 05day  (54) adult female 30day  (53) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    71 embryo 02-04hr    38 embryo 04-06hr    35 embryo 06-08hr    49 embryo 08-10hr    47 embryo 10-12hr    61 embryo 12-14hr    42 embryo 14-16hr    54 embryo 16-18hr    45 embryo 18-20hr    61 embryo 20-22hr    58 embryo 22-24hr    56 larva L1    44 larva L2    34 larva L3 12hr old    36 larva L3 puffstage 1-2    40 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    45 white prepupae new    38 white prepupae 12hr    44 white prepupae 24hr    45 pupae 2d postWPP    39 pupae 3d postWPP    39 pupae 4d postWPP    47 adult male 01day    62 adult male 05day    71 adult male 30day    62 adult female 01day    67 adult female 05day    54 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    71 embryo 02-04hr    38 embryo 04-06hr    35 embryo 06-08hr    49 embryo 08-10hr    47 embryo 10-12hr    61 embryo 12-14hr    42 embryo 14-16hr    54 embryo 16-18hr    45 embryo 18-20hr    61 embryo 20-22hr    58 embryo 22-24hr    56 larva L1    44 larva L2    34 larva L3 12hr old    36 larva L3 puffstage 1-2    40 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    45 white prepupae new    38 white prepupae 12hr    44 white prepupae 24hr    45 pupae 2d postWPP    39 pupae 3d postWPP    39 pupae 4d postWPP    47 adult male 01day    62 adult male 05day    71 adult male 30day    62 adult female 01day    67 adult female 05day    54 adult female 30day    53 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    71 embryo 02-04hr    38 embryo 04-06hr    35 embryo 06-08hr    49 embryo 08-10hr    47 embryo 10-12hr    61 embryo 12-14hr    42 embryo 14-16hr    54 embryo 16-18hr    45 embryo 18-20hr    61 embryo 20-22hr    58 embryo 22-24hr    56 larva L1    44 larva L2    34 larva L3 12hr old    36 larva L3 puffstage 1-2    40 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    45 white prepupae new    38 white prepupae 12hr    44 white prepupae 24hr    45 pupae 2d postWPP    39 pupae 3d postWPP    39 pupae 4d postWPP    47 adult male 01day    62 adult male 05day    71 adult male 30day    62 adult female 01day    67 adult female 05day    54 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (71) embryo 02-04hr  (38) embryo 04-06hr  (35) embryo 06-08hr  (49) embryo 08-10hr  (47) embryo 10-12hr  (61) embryo 12-14hr  (42) embryo 14-16hr  (54) embryo 16-18hr  (45) embryo 18-20hr  (61) embryo 20-22hr  (58) embryo 22-24hr  (56) larva L1  (44) larva L2  (34) larva L3 12hr old  (36) larva L3 puffstage 1-2  (40) larva L3 puffstage 3-6  (48) larva L3 puffstage 7-9  (45) white prepupae new  (38) white prepupae 12hr  (44) white prepupae 24hr  (45) pupae 2d postWPP  (39) pupae 3d postWPP  (39) pupae 4d postWPP  (47) adult male 01day  (62) adult male 05day  (71) adult male 30day  (62) adult female 01day  (67) adult female 05day  (54) adult female 30day  (53) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    71 embryo 02-04hr    38 embryo 04-06hr    35 embryo 06-08hr    49 embryo 08-10hr    47 embryo 10-12hr    61 embryo 12-14hr    42 embryo 14-16hr    54 embryo 16-18hr    45 embryo 18-20hr    61 embryo 20-22hr    58 embryo 22-24hr    56 larva L1    44 larva L2    34 larva L3 12hr old    36 larva L3 puffstage 1-2    40 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    45 white prepupae new    38 white prepupae 12hr    44 white prepupae 24hr    45 pupae 2d postWPP    39 pupae 3d postWPP    39 pupae 4d postWPP    47 adult male 01day    62 adult male 05day    71 adult male 30day    62 adult female 01day    67 adult female 05day    54 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    71 embryo 02-04hr    38 embryo 04-06hr    35 embryo 06-08hr    49 embryo 08-10hr    47 embryo 10-12hr    61 embryo 12-14hr    42 embryo 14-16hr    54 embryo 16-18hr    45 embryo 18-20hr    61 embryo 20-22hr    58 embryo 22-24hr    56 larva L1    44 larva L2    34 larva L3 12hr old    36 larva L3 puffstage 1-2    40 larva L3 puffstage 3-6    48 larva L3 puffstage 7-9    45 white prepupae new    38 white prepupae 12hr    44 white prepupae 24hr    45 pupae 2d postWPP    39 pupae 3d postWPP    39 pupae 4d postWPP    47 adult male 01day    62 adult male 05day    71 adult male 30day    62 adult female 01day    67 adult female 05day    54 adult female 30day    53 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    42 embryonic 1182-4H    49 embryonic GM2    29 embryonic Kc167    26 embryonic S1    41 embryonic S3    35 leg disc CME L1    41 wing disc CME-W2    57 wing disc ML-DmD8    59 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    61 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    31 ovary fGS/OSS    37 ovary OSC    31 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (74) Schneider line 2 Sg4  (42) embryonic 1182-4H  (49) embryonic GM2  (29) embryonic Kc167  26 embryonic S1  (41) embryonic S3  (35) leg disc CME L1  (41) wing disc CME-W2  (57) wing disc ML-DmD8  (59) wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21  (61) wing disc ML-DmD32  (40) haltere disc ML-DmD17-c3  (60) eye-antennal disc ML-DmD11  (48) antennal disc ML-DmD20-c5  (38) mixed discs ML-DmD4-c1  (39) CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2  (30) tumorous blood cells mbn2  (31) ovary fGS/OSS  (37) ovary OSC  (31) ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    42 embryonic 1182-4H    49 embryonic GM2    29 embryonic Kc167    26 embryonic S1    41 embryonic S3    35 leg disc CME L1    41 wing disc CME-W2    57 wing disc ML-DmD8    59 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    61 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    31 ovary fGS/OSS    37 ovary OSC    31 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    42 embryonic 1182-4H    49 embryonic GM2    29 embryonic Kc167    26 embryonic S1    41 embryonic S3    35 leg disc CME L1    41 wing disc CME-W2    57 wing disc ML-DmD8    59 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    61 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    31 ovary fGS/OSS    37 ovary OSC    31 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    42 embryonic 1182-4H    49 embryonic GM2    29 embryonic Kc167    26 embryonic S1    41 embryonic S3    35 leg disc CME L1    41 wing disc CME-W2    57 wing disc ML-DmD8    59 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    61 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    31 ovary fGS/OSS    37 ovary OSC    31 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (74) Schneider line 2 Sg4  (42) embryonic 1182-4H  (49) embryonic GM2  29 embryonic Kc167  26 embryonic S1  (41) embryonic S3  (35) leg disc CME L1  (41) wing disc CME-W2  (57) wing disc ML-DmD8  (59) wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21  (61) wing disc ML-DmD32  (40) haltere disc ML-DmD17-c3  (60) eye-antennal disc ML-DmD11  (48) antennal disc ML-DmD20-c5  (38) mixed discs ML-DmD4-c1  (39) CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2  30 tumorous blood cells mbn2  31 ovary fGS/OSS  (37) ovary OSC  31 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    42 embryonic 1182-4H    49 embryonic GM2    29 embryonic Kc167    26 embryonic S1    41 embryonic S3    35 leg disc CME L1    41 wing disc CME-W2    57 wing disc ML-DmD8    59 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    61 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    31 ovary fGS/OSS    37 ovary OSC    31 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    74 Schneider line 2 Sg4    42 embryonic 1182-4H    49 embryonic GM2    29 embryonic Kc167    26 embryonic S1    41 embryonic S3    35 leg disc CME L1    41 wing disc CME-W2    57 wing disc ML-DmD8    59 wing disc ML-DmD9    21 wing disc ML-DmD16-c3    15 wing disc ML-DmD21    61 wing disc ML-DmD32    40 haltere disc ML-DmD17-c3    60 eye-antennal disc ML-DmD11    48 antennal disc ML-DmD20-c5    38 mixed discs ML-DmD4-c1    39 CNS ML-DmBG1-c1    13 CNS ML-DmBG2-c2    30 tumorous blood cells mbn2    31 ovary fGS/OSS    37 ovary OSC    31 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    39 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult  (29) Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3  (41) Ethanol 5% 3 hrs, larvae L3  26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (31) Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (39) Paraquat 10 mM 48 hrs, 4-day adult  (47) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    39 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    39 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    39 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult  29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3  (41) Ethanol 5% 3 hrs, larvae L3  26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult  (39) Paraquat 10 mM 48 hrs, 4-day adult  (47) Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    39 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    17 heat shock, 4-day adult    29 Cadmium 50 mM 6 hrs, larvae L3    21 Cadmium 50 mM 12 hrs, larvae L3    15 Cadmium 50 mM 48 hrs, 4-day adult    22 Cadmium 100 mM 48 hrs, 4-day adult    25 Copper 0.5 mM 12 hrs, larvae L3    18 Copper 15 mM 48 hrs, 4-day adult    12 Zinc 5 mM 12 hrs, larvae L3    11 Zinc 4.5 mM 48 hrs, 4-day adult    14 Ethanol 2.5% 3 hrs, larvae L3    41 Ethanol 5% 3 hrs, larvae L3    26 Ethanol 10% 3 hrs, larvae L3    20 Caffeine 1.5 mg/ml 4 hrs, larvae L3    24 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    31 Caffeine 25 mg/ml 48 hrs, 4-day adult    18 Paraquat 5 mM 48 hrs, 4-day adult    39 Paraquat 10 mM 48 hrs, 4-day adult    47 Rotenone 2 μg 12 hrs, larvae L3    18 Rotenone 8 μg 12 hrs, larvae L3    24 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_26 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023169 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3051-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage | maternal effect SNF1AD1 lethal | larval stage SNF1A3 lethal | larval stage, with Scer\GAL4αTub84B.PL SNF1AdsRNA.Scer\UAS lethal | P-stage SNF1AΔ39 lethal | pupal stage SNF1AD1 lethal | recessive | second instar larval stage SNF1A1 SNF1A2 SNF1A3 lethal | third instar larval stage SNF1AD39 SNF1AΔ39 viable, with Scer\GAL4pnr-MD237 SNF1AGD736 Other Phenotypes Allele cell cycle defective SNF1A1 cell polarity defective SNF1A1 cytokinesis defective SNF1A1 developmental rate defective | second instar larval stage SNF1AΔ39 developmental rate defective | third instar larval stage SNF1AΔ39 feeding behavior defective, with Scer\GAL4Ubi SNF1AK57A.Scer\UAS hyperactive | nutrition conditional, with Scer\GAL4Ubi SNF1AK57A.Scer\UAS immune response defective | dominant SNF1A3 locomotor rhythm defective | nutrition conditional, with Scer\GAL4Ubi SNF1AK57A.Scer\UAS mitotic cell cycle defective | embryonic stage | maternal effect SNF1AD1 mitotic cell cycle defective | larval stage SNF1AD1 neuroanatomy defective SNF1A3 neuroanatomy defective, with Scer\GAL4109(2)80 SNF1AdsRNA.Scer\UAS SNF1AK57A.Scer\UAS short lived, with Scer\GAL4Ubi SNF1AK57A.Scer\UAS short lived | nutrition conditional, with Scer\GAL4hs.PB SNF1AdsRNA.Scer\UAS SNF1AK57A.Scer\UAS small body SNF1A3 small body | larval stage SNF1A1 small body | second instar larval stage SNF1AΔ39 small body | third instar larval stage SNF1AΔ39 starvation stress response defective SNF1A3 starvation stress response defective, with Scer\GAL4hs.PB SNF1AdsRNA.Scer\UAS SNF1AK57A.Scer\UAS starvation stress response defective, with Scer\GAL4Ubi SNF1AK57A.Scer\UAS starvation stress response defective, with SNF1AK57A.Scer\UAS, Scer\GAL4hs.PB SNF1AScer\UAS.cMa starvation stress response defective, with SNF1AScer\UAS.cMa, Scer\GAL4hs.PB SNF1AK57A.Scer\UAS stress response defective | dominant SNF1AD39 Phenotype manifest in Allele centrosome SNF1A1 circular visceral muscle fiber SNF1AΔ39 class IV dendritic arborizing neuron SNF1A3 class IV dendritic arborizing neuron, with Scer\GAL4109(2)80 SNF1AdsRNA.Scer\UAS SNF1AK57A.Scer\UAS denticle belt | maternal effect SNF1AD1 embryonic/first instar larval cuticle | maternal effect SNF1AD1 embryonic/larval digestive system SNF1AΔ39 embryonic/larval fat body SNF1AΔ39 embryonic/larval midgut SNF1AΔ39 embryonic epidermis | maternal effect SNF1AD1 follicle cell | somatic clone SNF1A3 hindgut muscle SNF1AΔ39 hindgut visceral muscle fiber SNF1AΔ39 longitudinal visceral muscle fiber SNF1AΔ39 midgut muscle SNF1AΔ39 neuroblast SNF1A1 oenocyte SNF1A3 oenocyte, with Scer\GAL4Ubi SNF1AK57A.Scer\UAS plasma membrane & dendrite SNF1A3 sensory neuron SNF1A3 spindle SNF1A1 stress fiber & follicle cell | somatic clone SNF1A3
Classical Alleles ( 9 )
For All Classical Alleles Show
Allele of SNF1A Class Mutagen Stocks Known lesion SNF1A1 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes SNF1A3 amorphic allele - genetic evidence ethyl methanesulfonate 1 Yes SNF1A2 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes SNF1AD1 P-element activity 0 Yes SNF1AD2 P-element activity 0 Yes SNF1AD39 P-element activity 0 Yes SNF1AG505 0 -- SNF1Aunspecified 0 -- SNF1AΔ39 P-element activity 0 Yes
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of SNF1A Class Mutagen Stocks Known lesion SNF1AScer\UAS.cMa in vitro construct - regulatory fusion 2 Yes SNF1AScer\UAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes SNF1AGD736 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SNF1AGL00004 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SNF1AHMS00362 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SNF1AJF01951 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SNF1AK57A.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 1 Yes SNF1AKK102684 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes SNF1AT184D.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement 1 Yes SNF1Aa.cPa in vitro construct - RNAi 0 Yes SNF1AcPa in vitro construct - RNAi 0 Yes SNF1AdsRNA.Scer\UAS in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes SNF1AK56R.Scer\UAS in vitro construct - regulatory fusion in vitro construct - amino acid replacement in vitro construct - site directed mutagenesis 0 Yes SNF1AKR.Scer\UAS in vitro construct - regulatory fusion 0 Yes SNF1ANIG.3051R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes SNF1AScer\UAS.cBa in vitro construct - regulatory fusion 0 Yes SNF1AScer\UAS.cKa in vitro construct - regulatory fusion 0 -- SNF1AScer\UAS.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SNF1ATD.Scer\UAS in vitro construct - amino acid replacement in vitro construct - regulatory fusion 0 Yes SNF1AWT.Scer\UAS in vitro construct - regulatory fusion 0 Yes SNF1AΔC.Scer\UAS.T:Disc\RFP-mCherry in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes SNF1AΔC.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(1)Exel6227 (Ryder, 2004.4.26) Df(1)SNF1AΔ39 (Bland et al., 2010)
Partially disrupted in	Df(1)SNF1AΔ39 (Bland et al., 2010)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD736} NA P{KK102684} NA P{NIG.3051R} NA P{TRiP.GL00004} NA P{TRiP.HMS00362} NA P{TRiP.JF01951} NA P{UAS-GFP-SNF1A} NA P{UAS-mCherry-SNF1A.ΔC} NA P{UAS-mCherry-SNF1A} NA P{UAS-SNF1A.B} NA P{UAS-SNF1A.dsRNA} NA P{UAS-SNF1A.K56R} NA P{UAS-SNF1A.K57A} NA P{UAS-SNF1A.K} NA P{UAS-SNF1A.KR} NA P{UAS-SNF1A.M} NA P{UAS-SNF1A.T184D} NA P{UAS-SNF1A.TD} NA P{UAS-SNF1A.WT} NA P{UAS-SNF1A.ΔC} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}SNF1AG505 NA
Gene Ontology: Function, Process & Cellular Component ( 20 unique terms )
Terms Based on Experimental Evidence ( 15 terms )
Molecular Function
CV term Evidence References
AMP-activated protein kinase activity inferred from mutant phenotype (Pan and Hardie, 2002)
Biological Process
CV term Evidence References
axon guidance inferred from genetic interaction with Rheb (Dimitroff et al., 2012) behavioral response to starvation inferred from mutant phenotype (Johnson et al., 2010) cellular response to starvation inferred from mutant phenotype (Johnson et al., 2010) establishment or maintenance of epithelial cell apical/basal polarity inferred from mutant phenotype (Lee et al., 2007) lipid metabolic process inferred from mutant phenotype (Johnson et al., 2010) mitotic cell cycle inferred from mutant phenotype (Lee et al., 2007) phagocytosis, engulfment inferred from mutant phenotype (Stroschein-Stevenson et al., 2006) phototaxis inferred from genetic interaction with Rheb (Dimitroff et al., 2012) protein phosphorylation inferred from mutant phenotype (Pan and Hardie, 2002) regulation of cell growth inferred from mutant phenotype (Bland et al., 2010) regulation of digestive system process inferred from mutant phenotype (Bland et al., 2010) sequestering of triglyceride inferred from mutant phenotype (Johnson et al., 2010)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Mirouse et al., 2007) nucleus inferred from direct assay (Pan and Hardie, 2002)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR002290, InterPro:IPR017441 (FlyBase Curators et al., 2004-) G-protein coupled receptor kinase activity inferred from sequence or structural similarity (Cassill et al., 1991) protein serine/threonine kinase activity inferred from sequence or structural similarity (Swiss-Prot Project Members, 1998.1.1) non-traceable author statement (Morrison et al., 2000)
Biological Process
CV term Evidence References
protein phosphorylation inferred from sequence or structural similarity (Cassill et al., 1991, Swiss-Prot Project Members, 1998.1.1) non-traceable author statement (Morrison et al., 2000) signal transduction inferred from sequence or structural similarity (Cassill et al., 1991)
Cellular Component
CV term Evidence References
AMP-activated protein kinase complex inferred by curator from GO:0004679 (Pan and Hardie, 2002)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
SNF1A - SNF4Aγ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions SNF1A allele Gene References unspecified lkb1 (Lee et al., 2007)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68705 DroID - A comprehensive database of gene and protein interactions. • FBgn0023169 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43904 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O18645
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG61NV5M Dipteran inclusive ortholog search EOG6G7C6N Insect inclusive ortholog search EOG6MCVVJ Arthropod inclusive ortholog search EOG66Q57Q Metazoa inclusive ortholog search EOG6N67Q2
Orthologs in Drosophila Species (EOG61NV5M)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\SNF1A     Drosophila simulans   Dsim\GD16554 Y   Drosophila sechellia   Dsec\GM19132 Y   Drosophila erecta   Dere\GG12850 Y   Drosophila yakuba   Dyak\GE16684 Y   Drosophila ananassae   Dana\GF21013 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA15892 Y   Drosophila persimilis   Dper\GL14247 Y   Drosophila willistoni   Dwil\GK16296 Y   Drosophila virilis   Dvir\GJ15670 Y   Drosophila mojavensis   Dmoj\GI16329 Y   Drosophila grimshawi   Dgri\GH12117 Y
Orthologs in non-Drosophila Dipterans (EOG6G7C6N)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007153   Anopheles gambiae Malaria mosquito  Agam\AGAP002686   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002898
Orthologs in non-Dipteran Insects (EOG6MCVVJ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\SNF1A   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG007650   Atta cephalotes Leafcutter ant  Acep\ACEP27116   Camponotus floridanus Florida carpenter ant  Cflo\CFLO17309   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL13558   Linepithema humile Argentine ant  Lhum\LH16947   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17985   Solenopsis invicta Red fire ant  Sinv\SINV23458   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005350   Bombyx mori Silkmoth  Bmor\BGIBMGA013139   Pediculus humanus Human body louse  Phum\PHUM029040   Tribolium castaneum Red flour beetle  Tcas\Snf1a
Orthologs in non-Insect Arthropods (EOG66Q57Q)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\tpi1a   Ixodes scapularis Deer tick  Isca\ISCW020637
Orthologs in non-Arthropod Metazoa (EOG6N67Q2)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\T01C8.1   Caenorhabditis elegans Nematode  Cele\aak-1   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_010280gn   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_020337gn   Danio rerio Zebrafish  Drer\prkaa2   Danio rerio Zebrafish  Drer\prkaa1   Xenopus tropicalis Western clawed frog  Xtro\prkaa1   Xenopus tropicalis Western clawed frog  Xtro\prkaa2   Gallus gallus Domestic chicken  Ggal\PRKAA2   Gallus gallus Domestic chicken  Ggal\ENSGALG00000014832   Mus musculus House mouse  Mmus\Prkaa1   Mus musculus House mouse  Mmus\Prkaa2   Rattus norvegicus Norway rat  Rnor\Prkaa1   Rattus norvegicus Norway rat  Rnor\Prkaa2   Homo sapiens Human  Hsap\PRKAA2   Homo sapiens Human  Hsap\PRKAA1
Human Orthologs (2)
Gene OMIM HGNC Hsap\PRKAA2     Hsap\PRKAA1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD16554 Drosophila sechellia Dsec\GM19132 Drosophila erecta Dere\GG12850 Drosophila yakuba Dyak\GE16684 Drosophila ananassae Dana\GF21013 Drosophila pseudoobscura pseudoobscura Dpse\GA15892 Drosophila persimilis Dper\GL14247 Drosophila willistoni Dwil\GK16296 Drosophila virilis Dvir\GJ15670 Drosophila mojavensis Dmoj\GI16329 Drosophila grimshawi Dgri\GH12117
Stocks & Reagents
Stocks Listed in FlyBase ( 13 )
Bloomington	33830 SNF1A1/FM7i 33831 SNF1A3/FM7i 32108 w1118; P{UAS-SNF1A.M}2 32112 w1118; P{UAS-SNF1A.K57A}2 32109 w1118; P{UAS-mCherry-SNF1A}2 32182 w1118; P{UAS-mCherry-SNF1A}3 32110 w1118; P{UAS-SNF1A.T184D}2/CyO 25931 y1 v1; P{TRiP.JF01951}attP2 33822 w1118; P{UAS-SNF1A.M}3/TM6C, Sb1 35137 y1 sc* v1; P{TRiP.GL00004}attP2
VDRC	v106200 P{KK102684}VIE-260B v1827 w1118; P{GD736}v1827
	More stocks available...
Genomic Clones ( 2 )
	BACR09H15 BACR30G24 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 106 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH12596 LP06206
Other clones	FI03728
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT07322 GH02732 GH02831 GH03262 GH04642 GH05909 GH06150 GH07170 GH08222 GH10370 GH11306 GH12837 GH13962 GH16225 GH17718 GH20217 GH25958 GM07749 GM10593 LD05233 LD10636 LD14193 LD21307 LP02575 RE06422 RE12969 RE15180 RE25791 RE28356 RE31518 RE45068 RE57304 RE65705 RH03740 RH18158 RH22610 RH25895 RH28959 RH42096 RH61756 SD06379 SD09578 SD12866 SD18880 SD18909 SD24808
Other clones	10922 54097 103196 117924 163994 214537.20 569153 CK01.9D.E5 CK01.78A.D4 Dmel_dig_016594_0686_1683 EC052916 EC053856 EC055258 EK060846 EK111057 EK172806 EK179357 EK237749 EK268420 IP01833 IP05225 IP05325 IP05525 IP10563 IP13288 RE01007 RP004079878 RP004118664 RP004142331 RP004155350 RP004159072 RP004160658 RP004190625 RP004201090 RP004201111 RP004206544 RP004207464 RP004214959 RP004220361 RP004237007 RP004246886 RP004251547 RP004257679 RP004260980 RP004267223 RP004304062 RP004351225 RP004415879 RP004418970 RP004428455 RP004441178 RP004454541 RP004456075 RP004480701 RP004481224 RP004483465 RP004526708
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0023169 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43904
Antibody Information
	polyclonal (Bland et al., 2010)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: SNF1A CG3051 (Bayraktaroglu, 2000.4.12) Source for merge of: SNF1A Gprk4 (Misra, 2000.7.28)
Additional comments
Other Comments
	Reduced SNF1A signaling leads to hypersensitivity to starvation conditions as measured by lifespan and locomotor activity. (Johnson et al., 2010) Reduced SNF1A function results in hyperphagia. (Johnson et al., 2010) SNF1A is required to maintain epithelial cell polarity in the follicle cell epithelium under energetic stress. (Mirouse et al., 2007) S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells. (Stroschein-Stevenson et al., 2006) When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, an increase in the proportion of S phase cells, an increase in the proportion of G2/M phase cells, a whole range of mitotic abnormalities, and centrosome abnormalities are seen. (Bettencourt-Dias et al., 2004)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057965 NM_166880 NM_206604 RefSeq (Proteins) • NP_477313 NP_726730 NP_996327 DDBJ / EMBL / GenBank • AAB71397 AAB71398 AAD55435 AAF45614 AAN09043 AAS65245 AAV36959 AF020309 AF020310 AF181649 AI107989 AI134993 AI402294 AL023893 BT016074 Entrez Gene - A searchable database of RefSeq genes. • 43904 UniProtKB/TrEMBL • O18645 Q5U118 Q7KK41
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Serine/threonine- / dual specificity protein kinase, catalytic domain (IPR002290) Serine/threonine-protein kinase, active site (IPR008271) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441)
Linkouts
	BioGRID - A database of protein and genetic interactions • 68705 DroID - A comprehensive database of gene and protein interactions. • FBgn0023169 DRSC - Results from RNAi screens. • FBgn0023169 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023169 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3051-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0023169 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43904 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /genebrief/snf1a_ampk.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43904 modMine - Data generated by the modENCODE project. • FBgn0023169 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • O18645
Synonyms & Secondary IDs ( 22 )
Reported As
Symbol Synonym	AMPK (Pan et al., 2004, Edgar, 2006, McBride et al., 2006, Spasic et al., 2007, Hoshizaki and Gibbs, 2007, Libert, 2007, Lee et al., 2007, Brenman et al., 2008, Wang et al., 2011, Wang et al., 2007, Mirouse et al., 2009, Lee et al., 2010, Mirouse et al., 2009, Dimitroff et al., 2012, Slack et al., 2012, Wang et al., 2012, Onyenwoke et al., 2012) ampk (Amin et al., 2009) AMPKalpha (Frei et al., 2007) AMPKα (Moser et al., 2010, Kazgan et al., 2010, Choi et al., 2011, Johnson et al., 2010, Andersen et al., 2012) ampkα (Amin et al., 2009, Mirouse et al., 2007, Chakrabarti et al., 2012) CG3051 (Benos, 2000.12.13, Morrison et al., 2000, Mirouse et al., 2007, Sun et al., 2010, Tohyama and Yamaguchi, 2010, Perkins et al., 1.15.2009, Keleman et al., 2009.8.5, Bland et al., 2010, Ni et al., 2010.12.1, Choi et al., 2011, Ni et al., 2011.7.19) dAMPKa (Bland et al., 2007) dAMPKα (Tohyama and Yamaguchi, 2010, Bland et al., 2010) DmAMPK α (Pan and Hardie, 2002) EG:132E8.2 (Benos et al., 2001, Benos, 2000.12.13, ) FBgn0023169 (Pan and Hardie, 2002) Gprk-4 (Cassill et al., 1991) Gprk4   snf1a (Andolfatto and Wall, 2003) SNF1A (Stroschein-Stevenson et al., 2006, Sun et al., 2010, Moser et al., 2010, Stofanko et al., 2008, Fernández-Ayala et al., 2010, Zhou et al., 2008, Brenman, 2010.9.17, Japanese National Institute of Genetics, 2012.5.21, Lindquist et al., 2011)
Name Synonym	5'monophosphate-activated kinase (Mirouse et al., 2007) adenosine monophosphate-activated protein kinase (Lee et al., 2010) AMP-activated kinase (Wang et al., 2012) AMP-activated protein kinase (Spasic et al., 2007, Lee et al., 2007, Baehrecke, 2006, Mirouse et al., 2009, Onyenwoke et al., 2012) AMP-dependent kinase (Edgar, 2006) G protein-coupled receptor kinase 4   SNF1A/AMP-activated protein kinase
Secondary FlyBase IDs
	FBgn0004836 FBgn0029574
References ( 70 )
Generate a list of	All references relating to this gene ( 70 )
List References by type	Research paper  ( 33 ) Supplementary material  ( 7 ) Review  ( 4 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 4 ) FlyBase analysis  ( 2 ) Conference report  ( 2 ) Stock list  ( 1 ) Protein sequence record  ( 1 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 10 )
	Andersen et al., 2012, Dev. Biol. 363(1): 258--265 Sgt1 acts via an LKB1/AMPK pathway to establish cortical polarity in larval neuroblasts. [FBrf0217488] Chakrabarti et al., 2012, Cell Host Microbe 12(1): 60--70 Infection-induced host translational blockage inhibits immune responses and epithelial renewal in the Drosophila gut. [FBrf0218984] Dimitroff et al., 2012, PLoS ONE 7(2): e30722 Diet and Energy-Sensing Inputs Affect TorC1-Mediated Axon Misrouting but Not TorC2-Directed Synapse Growth in a Drosophila Model of Tuberous Sclerosis. [FBrf0217461] Ng et al., 2012, J. Neurosci. 32(41): 14311--14317 AMP Kinase Activation Mitigates Dopaminergic Dysfunction and Mitochondrial Abnormalities in Drosophila Models of Parkinson's Disease. [FBrf0219671] Onyenwoke et al., 2012, Mol. Biol. Cell 23(2): 381--389 AMPK directly inhibits NDPK through a phosphoserine switch to maintain cellular homeostasis. [FBrf0218833] Slack et al., 2012, PLoS ONE 7(10): e47699 Activation of AMPK by the Putative Dietary Restriction Mimetic Metformin Is Insufficient to Extend Lifespan in Drosophila. [FBrf0219763] Wang et al., 2012, Nat. Commun. 3: 769 Drosophila follicle stem cells are regulated by proliferation and niche adhesion as well as mitochondria and ROS. [FBrf0217898] Choi et al., 2011, J. Biol. Chem. 286(4): 2658--2664 Drosophila Salt-inducible Kinase (SIK) Regulates Starvation Resistance through cAMP-response Element-binding Protein (CREB)-regulated Transcription Coactivator (CRTC). [FBrf0212827] Lindquist et al., 2011, Genome Res. 21(3): 433--446 Genome-scale RNAi on living-cell microarrays identifies novel regulators of Drosophila melanogaster TORC1-S6K pathway signaling. [FBrf0213199] Wang et al., 2011, Cell 145(4): 596--606 A hormone-dependent module regulating energy balance. [FBrf0213699] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...