DRhoGEF2, RhoGEF, shar, DRhoGEF, l(2)04291
induces contractile cell shape changes by stimulating myosin II via the Rho1 pathway - location at the actin cortex regulated by interaction with the microtubule plus-end tracking protein EB1
Please see the JBrowse view of Dmel\RhoGEF2 for information on other features
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AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.50
10.5, 8.5 (northern blot)
9.5 (northern blot)
The group(s) of polypeptides indicated below share identical sequence to each other.
2559 (aa); 281 (kD predicted)
2559 (aa); 284 (kD predicted)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoGEF2 using the Feature Mapper tool.
The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).
Expression in stage P3 pupal leg discs is restricted to presumptive tarsal joints.
The RhoGEF2 transcript is abundant in syncytial blastoderm embryos, continues to be detected during gastrulation, and is not detected after germ band extension.
JBrowse - Visual display of RNA-Seq signals
View Dmel\RhoGEF2 in JBrowseMaps at 2-80 to 2-85.
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
RhoGEF2 is required for furrow canal formation during cellularisation.
RhoGEF2 activity is required for actin organization and acto-myosin contractility during somatic nuclear divisions, pole cell formation and cellularization of syncytial blastoderm embryos.
RhoGEF2 is required for epithelial folding and invagination.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
Mutations in RhoGEF2 disrupt the coordinates induction of cell shape changes.
RhoGEF2 is essential for the invagination of mesodermal and endodermal primordia during gastrulation.
RhoGEF2 is essential for directing the cell shape changes associated with gastrulation.
Embryos lacking RhoGEF2 (generated as germline clones) show inappropriate lateral folds.
The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.
Named "shar pei" after the breed of dog characterized by numerous skin folds, to reflect the phenotype of mutant embryos.
