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Gene Dmel\RhoGEF2

General Information
Symbol	Dmel\RhoGEF2	Species	D. melanogaster
Name	RhoGEF2	Annotation symbol	CG9635
Feature type	protein_coding_gene	FlyBase ID	FBgn0023172
Gene Model Status	Current	Stock availability	17 publicly available
Also Known As	DRhoGEF2, DRhoGEF, RhoGEF, Rho-GEF2
Genomic Location
Chromosome (arm)	2R	Recombination map	2-
Cytogenetic map	53E4-53F1	Sequence location	2R:12,914,746..12,932,158 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene RhoGEF2 is referred to in FlyBase by the symbol Dmel\RhoGEF2 (CG9635, FBgn0023172). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: diacylglycerol binding; Rho guanyl-nucleotide exchange factor activity. There is experimental evidence for 15 unique biological process terms, many of which group under: cellular component organization or biogenesis; biological regulation; morphogenesis of embryonic epithelium; anatomical structure formation involved in morphogenesis; regulation of developmental process; multicellular organismal development; actin filament-based process; gastrulation; regulation of cell morphogenesis; pole cell development; morphogenesis of an epithelial fold; organelle organization; embryonic development via the syncytial blastoderm; open tracheal system development. 42 alleles are reported. The phenotypes of these alleles are annotated with: presumptive embryonic salivary gland; wing disc; epithelial cell; posterior spiracle primordium; wing; mesoderm anlage; ventral midline. It has 4 annotated transcripts and 4 annotated polypeptides. Protein features are: Dbl homology (DH) domain; PDZ/DHR/GLGF; Pleckstrin homology domain; Pleckstrin homology-type; Protein kinase C-like, phorbol ester/diacylglycerol binding; Regulator of G protein signalling superfamily; Regulator of G protein signalling-like fold; Rho GTP exchange factor. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult hindgut. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1634904_s_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of RhoGEF2. Gene sequence location is 2R:12914746..12932158.
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10	Controlled Vocabulary Terms termination of G-protein coupled receptor signaling pathway References Bakal et al., 2007, Science 316(5832): 1753--1756
FB2012_01	Sequence features HFA07531 BKN20815
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 53E4-53F1   Limits computationally determined from genome sequence between P{lacW}l(2)k11405k11405&P{EP}EP641 and P{lacW}l(2)k10815k10815
Experimentally Determined Cytological Location
Cytogenetic map Notes References 53F1-53D2     (Haecker, 1997.10.30) 53F1-53F2   53F1--3   (Barrett, 1997.11.3) 53F1-53F2   (determined by in situ hybridisation)   (Spradling et al., 1999) 53F-53F   (determined by in situ hybridisation)   (Settleman and Barrett, 1997.8.8) 53F1-53F2   (determined by in situ hybridisation)   (Perrimon et al., 1996) 53F1-53F2     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location	2- (Settleman and Barrett, 1997.8.8)
Left of (cM)	px (Barrett et al., 1997)
Right of (cM)	c (Barrett et al., 1997)
Notes	Maps at 2-80 to 2-85. (Barrett et al., 1997)
Gene Model & Products
Please see the GBrowse view of Dmel\RhoGEF2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) RhoGEF2-RD FBtr0086971  NM_057969   8424   2559 RhoGEF2-RE FBtr0086969  NM_206147   7373   2205 RhoGEF2-RF FBtr0086970  NM_206146   8435   2559 RhoGEF2-RG FBtr0305083     8429   2557
Additional Transcript Data & Comments
Reported size (kB)	10.5, 8.5 (northern blot) (Hacker and Perrimon, 1998) 9.5 (northern blot) (Barrett et al., 1997)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein RhoGEF2-PD   FBpp0086125   280.8   2559   6.76     NP_477317   AAM68489 RhoGEF2-PE   FBpp0086123   242.2   2205   6.37     NP_995869   AAF57915 RhoGEF2-PF   FBpp0086124   280.8   2559   6.76     NP_995868   AAM68490 RhoGEF2-PG   FBpp0293620   280.6   2557   6.76
Additional Polypeptide Data & Comments
Reported size (kDa)	2559 (aa); 284 (kD predicted) (Barrett et al., 1997) 2559 (aa); 281 (kD predicted) (Hacker and Perrimon, 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dbl homology (DH) domain (IPR000219) PDZ/DHR/GLGF (IPR001478) Pleckstrin homology domain (IPR001849) Protein kinase C-like, phorbol ester/diacylglycerol binding (IPR002219) Pleckstrin homology-type (IPR011993) Regulator of G protein signalling-like fold (IPR015212) Rho GTP exchange factor (IPR015721) Regulator of G protein signalling superfamily (IPR016137)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA141526   AA697827   AE013599 AAF57915   AE013599 AAM68489   AE013599 AAM68490   AC004335   AC005717   AF031930 AAB88816   AF032870 AAC38820   AI532843   AI534196   AI542863   AQ025625   AQ073581   AW944363   AY069804 AAL39949   BG636168   BG636529   BI353759   BI628377   BI639886   BT125864   CL610210   CO177700   CO187072   CO190881   CO196443   CO276156   CO280850   CO293281   CO306089   CO318050   CO322124   CO327049   CO328330   CO337957   CO338905   CO342291   CO342378   CO344696   CZ481539   CZ481540   EC052052   EC054053   EC054547   EC055049   EC055491   EC055801   EC056006   EC056108   EC056380   EC059639   EC061933   EC063742   EC069642   EC078197   EC194594   EC194599   EC198896   EC200355   EC203122   EC203334   EC208906   EC209746   EC211843   EC217824   EC225340   EC240163   EC241039   EC253183   EC255136   EC256624   EC258342   EC258849   EC261603   ES693173   ES693245   ES693251   EV578714   EV580213
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O44113 O44381 Q8T9E3 A1ZAN6 A1ZAN7
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 8   organism   (Hacker and Perrimon, 1998)
Additional Descriptive Data
	The RhoGEF2 transcript is abundant in syncytial blastoderm embryos, continues to be detected during gastrulation, and is not detected after germ band extension. (Hacker and Perrimon, 1998)
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0023172    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0023172 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of moderate expression. Peak expression observed at stages throughout embryogenesis, during late larval stages, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0023172 expression level Developmental Stage   Expression Level embryo 00-02hr    2797 embryo 02-04hr    839 embryo 04-06hr    2015 embryo 06-08hr    2399 embryo 08-10hr    2046 embryo 10-12hr    1244 embryo 12-14hr    1728 embryo 14-16hr    1443 embryo 16-18hr    1418 embryo 18-20hr    1615 embryo 20-22hr    1206 embryo 22-24hr    1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6    1923 larva L3 puffstage 7-9    2946 white prepupae new    2822 white prepupae 12hr    2156 white prepupae 24hr    2064 pupae 2d postWPP    1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day    1819 adult female 05day    2837 adult female 30day    2884 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2797) embryo 02-04hr  (839) embryo 04-06hr  (2015) embryo 06-08hr  (2399) embryo 08-10hr  (2046) embryo 10-12hr  (1244) embryo 12-14hr  (1728) embryo 14-16hr  (1443) embryo 16-18hr  (1418) embryo 18-20hr  (1615) embryo 20-22hr  (1206) embryo 22-24hr  (1418) larva L1  (840) larva L2  (798) larva L3 12hr old  (824) larva L3 puffstage 1-2  (1044) larva L3 puffstage 3-6  (1923) larva L3 puffstage 7-9  (2946) white prepupae new  (2822) white prepupae 12hr  (2156) white prepupae 24hr  (2064) pupae 2d postWPP  (1719) pupae 3d postWPP  (1046) pupae 4d postWPP  (978) adult male 01day  (1238) adult male 05day  (1232) adult male 30day  (1212) adult female 01day  (1819) adult female 05day  (2837) adult female 30day  (2884) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2797) embryo 02-04hr    839 embryo 04-06hr  (2015) embryo 06-08hr  (2399) embryo 08-10hr  (2046) embryo 10-12hr    1244 embryo 12-14hr  (1728) embryo 14-16hr  1443 embryo 16-18hr  1418 embryo 18-20hr  (1615) embryo 20-22hr    1206 embryo 22-24hr  1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6  (1923) larva L3 puffstage 7-9  (2946) white prepupae new  (2822) white prepupae 12hr  (2156) white prepupae 24hr  (2064) pupae 2d postWPP  (1719) pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day  (1819) adult female 05day  (2837) adult female 30day  (2884) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2797 embryo 02-04hr    839 embryo 04-06hr    2015 embryo 06-08hr    2399 embryo 08-10hr    2046 embryo 10-12hr    1244 embryo 12-14hr    1728 embryo 14-16hr    1443 embryo 16-18hr    1418 embryo 18-20hr    1615 embryo 20-22hr    1206 embryo 22-24hr    1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6    1923 larva L3 puffstage 7-9    2946 white prepupae new    2822 white prepupae 12hr    2156 white prepupae 24hr    2064 pupae 2d postWPP    1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day    1819 adult female 05day    2837 adult female 30day    2884 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2797 embryo 02-04hr    839 embryo 04-06hr    2015 embryo 06-08hr    2399 embryo 08-10hr    2046 embryo 10-12hr    1244 embryo 12-14hr    1728 embryo 14-16hr    1443 embryo 16-18hr    1418 embryo 18-20hr    1615 embryo 20-22hr    1206 embryo 22-24hr    1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6    1923 larva L3 puffstage 7-9    2946 white prepupae new    2822 white prepupae 12hr    2156 white prepupae 24hr    2064 pupae 2d postWPP    1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day    1819 adult female 05day    2837 adult female 30day    2884 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0023172 expression level Developmental Stage   Expression Level embryo 00-02hr    2797 embryo 02-04hr    839 embryo 04-06hr    2015 embryo 06-08hr    2399 embryo 08-10hr    2046 embryo 10-12hr    1244 embryo 12-14hr    1728 embryo 14-16hr    1443 embryo 16-18hr    1418 embryo 18-20hr    1615 embryo 20-22hr    1206 embryo 22-24hr    1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6    1923 larva L3 puffstage 7-9    2946 white prepupae new    2822 white prepupae 12hr    2156 white prepupae 24hr    2064 pupae 2d postWPP    1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day    1819 adult female 05day    2837 adult female 30day    2884 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2797) embryo 02-04hr  (839) embryo 04-06hr  (2015) embryo 06-08hr  (2399) embryo 08-10hr  (2046) embryo 10-12hr  (1244) embryo 12-14hr  (1728) embryo 14-16hr  (1443) embryo 16-18hr  (1418) embryo 18-20hr  (1615) embryo 20-22hr  (1206) embryo 22-24hr  (1418) larva L1  (840) larva L2  (798) larva L3 12hr old  (824) larva L3 puffstage 1-2  (1044) larva L3 puffstage 3-6  (1923) larva L3 puffstage 7-9  (2946) white prepupae new  (2822) white prepupae 12hr  (2156) white prepupae 24hr  (2064) pupae 2d postWPP  (1719) pupae 3d postWPP  (1046) pupae 4d postWPP  (978) adult male 01day  (1238) adult male 05day  (1232) adult male 30day  (1212) adult female 01day  (1819) adult female 05day  (2837) adult female 30day  (2884) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2797) embryo 02-04hr    839 embryo 04-06hr  2015 embryo 06-08hr  2399 embryo 08-10hr  2046 embryo 10-12hr    1244 embryo 12-14hr  1728 embryo 14-16hr  1443 embryo 16-18hr  1418 embryo 18-20hr  1615 embryo 20-22hr    1206 embryo 22-24hr  1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6  1923 larva L3 puffstage 7-9  (2946) white prepupae new  (2822) white prepupae 12hr  2156 white prepupae 24hr  2064 pupae 2d postWPP  1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day  1819 adult female 05day  (2837) adult female 30day  (2884) Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2797 embryo 02-04hr    839 embryo 04-06hr    2015 embryo 06-08hr    2399 embryo 08-10hr    2046 embryo 10-12hr    1244 embryo 12-14hr    1728 embryo 14-16hr    1443 embryo 16-18hr    1418 embryo 18-20hr    1615 embryo 20-22hr    1206 embryo 22-24hr    1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6    1923 larva L3 puffstage 7-9    2946 white prepupae new    2822 white prepupae 12hr    2156 white prepupae 24hr    2064 pupae 2d postWPP    1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day    1819 adult female 05day    2837 adult female 30day    2884 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2797 embryo 02-04hr    839 embryo 04-06hr    2015 embryo 06-08hr    2399 embryo 08-10hr    2046 embryo 10-12hr    1244 embryo 12-14hr    1728 embryo 14-16hr    1443 embryo 16-18hr    1418 embryo 18-20hr    1615 embryo 20-22hr    1206 embryo 22-24hr    1418 larva L1    840 larva L2    798 larva L3 12hr old    824 larva L3 puffstage 1-2    1044 larva L3 puffstage 3-6    1923 larva L3 puffstage 7-9    2946 white prepupae new    2822 white prepupae 12hr    2156 white prepupae 24hr    2064 pupae 2d postWPP    1719 pupae 3d postWPP    1046 pupae 4d postWPP    978 adult male 01day    1238 adult male 05day    1232 adult male 30day    1212 adult female 01day    1819 adult female 05day    2837 adult female 30day    2884 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0023172    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0023172 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Nearly all larval and adult tissues/organs expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult hindgut. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0023172 expression level Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut    507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut  507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut    507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut    507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0023172 expression level Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut    507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut  507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut    507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    285.575 Larval Midgut    385.7 Larval Hindgut    475.5 Larval Malpighian Tubules    366 Larval Fat Body    260.5 Larval Salivary Gland    285.1 Larval Trachea    243.5 Larval Carcass    325.525 Adult Head    235.7 Adult Eye    312.625 Adult Brain    199.4 Adult Thoracic-Abdominal Ganglion    158.4 Adult Crop    385.8 Adult Midgut    351.3 Adult Hindgut    507.3 Adult Malpighian Tubules    280.2 Adult Fat Body    265.5 Adult Salivary Gland    265.2 Adult Heart    230.725 Adult VirginFemale Spermatheca    374.6 Adult InseminatedFemale Spermatheca    330.1 Adult Ovary    428.5 Adult Testis    43.3 Adult Male Accessory Gland    200.1 Adult Carcass    268.3 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    235.7   NA  Eye    312.625   NA  Brain    199.4   285.575  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    158.4   NA  Crop    385.8   385.7  Midgut    351.3   475.5  Hindgut    507.3   366  Malpighian Tubules    280.2   260.5  Fat Body    265.5   285.1  Salivary Gland    265.2   NA  Heart    230.725   243.5  Trachea    NA   NA  VirginFemale Spermatheca    374.6   NA  InseminatedFemale Spermatheca    330.1   NA  Ovary    428.5   NA  Testis    43.3   NA  Male Accessory Gland    200.1   325.525  Carcass    268.3 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_23
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023172 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9635-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(2R)ED1) RhoGEF204291 lethal (with RhoGEF21.1) RhoGEF24.1 lethal (with RhoGEF24.1) RhoGEF21.1 RhoGEF24.4 lethal (with RhoGEF24.4) RhoGEF24.1 lethal | embryonic stage (with RhoGEF24.1) RhoGEF25.1 RhoGEF26.5 lethal | embryonic stage (with RhoGEF25.1) RhoGEF24.1 lethal | embryonic stage (with RhoGEF26.5) RhoGEF24.1 lethal | embryonic stage | maternal effect | recessive RhoGEF204291 lethal | embryonic stage | partially | rescuable maternal effect RhoGEF204291 lethal | first instar larval stage (with RhoGEF24.1) RhoGEF25.1 RhoGEF26.5 lethal | first instar larval stage (with RhoGEF25.1) RhoGEF24.1 lethal | first instar larval stage (with RhoGEF26.5) RhoGEF24.1 lethal | larval stage | recessive RhoGEF204291 lethal | partially (with RhoGEF24.1) RhoGEF2PX6 RhoGEF2PX10 lethal | partially (with RhoGEF2PX6) RhoGEF24.1 lethal | partially (with RhoGEF2PX10) RhoGEF24.1 lethal | recessive RhoGEF21.1 RhoGEF24.1 RhoGEF211-3 RhoGEF204291 viable RhoGEF2EY08391 Sterility Allele female sterile | germline clone RhoGEF2201 RhoGEF2350 fertile RhoGEF2EY08391 Other Phenotypes Allele cell shape defective RhoGEF21.1 RhoGEF2MtnA.T:Avic\GFP-EGFP cell shape defective | conditional | third instar larval stage, with Scer\GAL4ap-md544, Scer\GAL80ts.αTub84B RhoGEF2Scer\UAS.cUa mitotic cell cycle defective | embryonic stage | maternal effect RhoGEF204291 visible (with RhoGEF24.1) RhoGEF2PX6 RhoGEF2PX10 visible (with RhoGEF2PX6) RhoGEF24.1 visible (with RhoGEF2PX10) RhoGEF24.1 Phenotype manifest in Allele actin cytoskeleton | blastoderm stage | germline clone RhoGEF204291 actin filament & embryonic/larval tracheal system, with Scer\GAL4btl.PS RhoGEF2NIG.9635R amnioproctodeal invagination | germline clone RhoGEF21.1 RhoGEF24.1 anterior midgut primordium | germline clone | maternal effect RhoGEF204291 blastoderm embryo | germline clone RhoGEF204291 cortical actin cytoskeleton & syncytial blastoderm embryo | maternal effect RhoGEF204291 embryonic/first instar larval cuticle | germline clone | maternal effect | ventral RhoGEF204291 embryonic/first instar larval cuticle | germline clone | ventral RhoGEF204291 embryonic ventral epidermis | rescuable maternal effect RhoGEF204291 epithelial cell RhoGEF21.1 extended germ band embryo | germline clone RhoGEF21.1 RhoGEF24.1 extended germ band embryo | germline clone | maternal effect RhoGEF204291 furrow canal | germline clone RhoGEF204291 RhoGEF21.1 mesectoderm | germline clone RhoGEF21.1 RhoGEF24.1 mesoderm anlage RhoGEF24.1 nucleus | blastoderm stage | germline clone RhoGEF204291 pole cell & cytoskeleton | germ-line clone RhoGEF204291 pole cell | germline clone RhoGEF204291 posterior midgut primordium | germline clone | maternal effect RhoGEF204291 posterior spiracle primordium RhoGEF204291 presumptive embryonic salivary gland (with RhoGEF24.1) RhoGEF26.5 presumptive embryonic salivary gland (with RhoGEF26.5) RhoGEF24.1 stage 5 embryo | germline clone RhoGEF21.1 RhoGEF24.1 stomodeal invagination | germline clone RhoGEF21.1 RhoGEF24.1 ventral furrow | germline clone RhoGEF21.1 RhoGEF24.1 ventral midline RhoGEF204291 wing (with RhoGEF24.1) RhoGEF2PX10 RhoGEF2PX6 wing (with RhoGEF2PX6) RhoGEF24.1 wing (with RhoGEF2PX10) RhoGEF24.1 wing disc (with RhoGEF24.1) RhoGEF2PX6 wing disc (with RhoGEF2PX6) RhoGEF24.1 wing disc | conditional | third instar larval stage, with Scer\GAL4ap-md544, Scer\GAL80ts.αTub84B RhoGEF2Scer\UAS.cUa wing disc | somatic clone RhoGEF21.1
Classical Alleles ( 24 )
For All Classical Alleles Show
Allele of RhoGEF2 Class Mutagen Stocks Known lesion RhoGEF2e03784 piggyBac transposase 3 -- RhoGEF204291 loss of function allele P-element activity 1 Yes RhoGEF21.1 loss of function allele ethyl methanesulfonate 1 Yes RhoGEF24.1 loss of function allele ethyl methanesulfonate 1 Yes RhoGEF24.4 loss of function allele ethyl methanesulfonate 1 Yes RhoGEF25.1 antimorphic allele - genetic evidence ethyl methanesulfonate 1 Yes RhoGEF26.5 antimorphic allele - genetic evidence ethyl methanesulfonate 1 Yes RhoGEF29.6 ethyl methanesulfonate 1 -- RhoGEF2EY08391 P-element activity 1 -- RhoGEF2MB02788 1 -- RhoGEF211-3 ethyl methanesulfonate 0 -- RhoGEF22.3 ethyl methanesulfonate 0 -- RhoGEF22.6 ethyl methanesulfonate 0 -- RhoGEF22.9 ethyl methanesulfonate 0 -- RhoGEF2201 0 -- RhoGEF2350 0 -- RhoGEF23w18 hypomorphic allele - genetic evidence 0 -- RhoGEF25.2 ethyl methanesulfonate 0 -- RhoGEF28.1 ethyl methanesulfonate 0 -- RhoGEF28.9 ethyl methanesulfonate 0 -- RhoGEF2EP2432 P-element activity 0 -- RhoGEF2PX10 hypomorphic allele - genetic evidence P-element activity 0 Yes RhoGEF2PX6 hypomorphic allele - genetic evidence P-element activity 0 Yes RhoGEF2unspecified 0 --
Alleles Carried on Transgenic Constructs ( 18 )
For All Alleles Carried on Transgenic Constructs Show
Allele of RhoGEF2 Class Mutagen Stocks Known lesion RhoGEF2Scer\UAS.P\T.T:Uuuu\T7 in vitro construct - regulatory fusion in vitro construct - coding region fusion 2 Yes RhoGEF2DeltaPDZ.Scer\UAS.P\T.T:Uuuu\T7 in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - deletion 1 Yes RhoGEF2HMS01118 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RhoGEF2JF01747 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes RhoGEF2dsRNA.cRa in vitro construct - RNAi 0 Yes RhoGEF2GAGA.sqh.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes RhoGEF2GD14275 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RhoGEF2KK100719 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes RhoGEF2MtnA.T:Avic\GFP-EGFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RhoGEF2NIG.9635R in vitro construct - RNAi 0 -- RhoGEF2PDZ.Scer\UAS.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RhoGEF2PDZ.Scer\UAS.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RhoGEF2PDZ.T:Avic\GFP in vitro construct - coding region fusion 0 Yes RhoGEF2RE.Scer\UAS in vitro construct - regulatory fusion 0 Yes RhoGEF2Scer\UAS.cUa in vitro construct - regulatory fusion 0 Yes RhoGEF2Scer\UAS.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RhoGEF2sqh.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes RhoGEF2T1544A.sqh.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion in vitro construct - site directed mutagenesis 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)BSC49 (Deal-Herr and Cook, 2004.1.20) Df(2R)BSC331 (Christensen et al., 2007.10.29) Df(2R)BSC359 (Christensen and Cook, 2007.10.29) Df(2R)ED1 (Roote, 2002.9.18, Ryder et al., 2004, Ryder and Roote, 2004.11.10) Df(2R)Exel6065 (Ryder, 2004.4.26) Df(2R)P803-Δ15 (Bayer et al., 2003)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14275} NA P{KK100719} NA P{TRiP.HMS01118} NA P{TRiP.JF01747} NA P{UAS-RhoGEF2.4xPDZ.myc} NA P{UAS-RhoGEF2.4xPDZ.RFP} NA P{UAS-RhoGEF2.RFP} NA P{UAS-RhoGEF2.U} NA characterization construct P{NIG.9635R} NA P{sqh-RhoGEF2.6xmyc} NA P{sqh-RhoGEF2.GAGA.6xmyc} NA P{sqh-RhoGEF2.T1544A.6xmyc} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}RhoGEF2EP2432 NA P{EPgy2}RhoGEF2EY08391 NA P{GSV1}C79C79 NA miscellaneous insertions P{?PZ}RhoGEF2PX6 NA P{?PZ}RhoGEF2PX10 NA PBac{RB}RhoGEF2e03784 NA insertion of enhancer trap P{PZ}RhoGEF204291 No insertion of enhancer trap binary system Mi{ET1}ΡGEF2MB02788 No
Gene Ontology: Function, Process & Cellular Component ( 27 unique terms )
Terms Based on Experimental Evidence ( 22 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
actin cytoskeleton reorganization inferred from mutant phenotype (Padash Barmchi et al., 2005) actin filament organization inferred from mutant phenotype (Grosshans et al., 2005) anterior midgut invagination inferred from mutant phenotype (Barrett et al., 1997) cell elongation involved in imaginal disc-derived wing morphogenesis inferred from mutant phenotype (Widmann and Dahmann, 2009) cellularization inferred from mutant phenotype (Grosshans et al., 2005, Padash Barmchi et al., 2005) germ-band extension inferred from mutant phenotype (Barrett et al., 1997) morphogenesis of embryonic epithelium inferred from mutant phenotype (Dawes-Hoang et al., 2005) pole cell development inferred from mutant phenotype (Padash Barmchi et al., 2005) posterior midgut invagination inferred from mutant phenotype (Barrett et al., 1997) pseudocleavage involved in syncytial blastoderm formation inferred from mutant phenotype (Webb et al., 2009) regulation of axonogenesis inferred from genetic interaction with LIMK1 (Ng and Luo, 2004) regulation of cell shape inferred from direct assay (Nikolaidou and Barrett, 2002) inferred from mutant phenotype (Dawes-Hoang et al., 2005) regulation of embryonic cell shape inferred from genetic interaction with fog AND inferred from mutant phenotype (Barrett et al., 1997) spiracle morphogenesis, open tracheal system inferred from mutant phenotype (Simoes et al., 2006) ventral furrow formation inferred from mutant phenotype (Barrett et al., 1997, Fox and Peifer, 2007)
Cellular Component
CV term Evidence References
actin cap inferred from direct assay (Padash Barmchi et al., 2005) actomyosin contractile ring inferred from direct assay (Padash Barmchi et al., 2005) apical cortex inferred from direct assay (Padash Barmchi et al., 2005) apical part of cell inferred from direct assay (Padash Barmchi et al., 2005) apical plasma membrane inferred from direct assay (Padash Barmchi et al., 2005) basal part of cell inferred from direct assay (Padash Barmchi et al., 2005) lateral plasma membrane inferred from direct assay (Padash Barmchi et al., 2005)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
diacylglycerol binding inferred from sequence or structural similarity with InterPro:IPR002219 (FlyBase, 1992-) Rho guanyl-nucleotide exchange factor activity inferred from electronic annotation with InterPro:IPR000219, InterPro:IPR015212 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Barrett et al., 1997) non-traceable author statement (Haecker, 1997.10.30) traceable author statement (Leptin, 1999)
Biological Process
CV term Evidence References
gastrulation traceable author statement (Leptin, 1999) regulation of embryonic cell shape non-traceable author statement (Leptin, 1999) regulation of Rho protein signal transduction inferred from electronic annotation with InterPro:IPR000219 (FlyBase Curators et al., 2004-) termination of G-protein coupled receptor signaling pathway inferred from electronic annotation with InterPro:IPR016137 (FlyBase Curators et al., 2004-)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions RhoGEF2 allele Gene References RhoGEF21.1 fog (Barrett et al., 1997) LIMK1 (Ng and Luo, 2004) Rho1 (Barrett et al., 1997, Bayer et al., 2003) Sb (Bayer et al., 2003) zip (Halsell et al., 2000, Bayer et al., 2003) RhoGEF22.3 Rho1 (Barrett et al., 1997) RhoGEF22.6 Rho1 (Barrett et al., 1997) RhoGEF24.1 cta (Nikolaidou, 2004) fog (Barrett et al., 1997, Nikolaidou, 2004, Nikolaidou, 2004) LIMK1 (Ng and Luo, 2004) Rho1 (Bayer et al., 2003) Sb (Bayer et al., 2003) ssh (Ng and Luo, 2004) zip (Halsell et al., 2000, Bayer et al., 2003) RhoGEF26.5 fog (Nikolaidou, 2004) RhoGEF211-3 E(br)65 (Ward et al., 2003) E(br)444 (Ward et al., 2003) E(zip)12-5 (Bayer et al., 2003) E(zip)18-5 (Bayer et al., 2003) Rho1 (Ward et al., 2003, Bayer et al., 2003) Sb (Bayer et al., 2003) zip (Bayer et al., 2003) RhoGEF204291 btl (Zhu et al., 2005) E(br)121 (Ward et al., 2003) E(br)444 (Ward et al., 2003) flw (Vereshchagina et al., 2004) LIMK1 (Chen et al., 2004) Rho1 (Barrett et al., 1997, Bayer et al., 2003) Sb (Bayer et al., 2003) zip (Halsell et al., 2000, Mizuno et al., 2002, Bayer et al., 2003) RhoGEF2dsRNA.cRa cta (Rogers et al., 2004) RhoGEF2MtnA.T:Avic\GFP-EGFP Rho1 (Rogers et al., 2004) rok (Rogers et al., 2004) RhoGEF2PX6 cta (Nikolaidou, 2004) RhoGEF2PX10 cta (Nikolaidou, 2004) unspecified bnk (Padash Barmchi et al., 2005) E(zip)31-6 (Bayer et al., 2003) Rho1 (Barrett et al., 1997)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62615 DroID - A comprehensive database of gene and protein interactions. • FBgn0023172 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36915
Orthologs
Genome-wide drosophilid orthologs	Dana\GF11430 Dere\GG20653 Dgri\GH23156 Dmoj\GI21339 Dper\GL10452 Dpse\GA24373 Dsim\GD11240 Dvir\GJ19685 Dwil\GK21918 Dyak\GE11638
Curated drosophilid orthologs	Dpse\GA21930
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AGAP010767-PA Anopheles gambiae (mosquito) XP_393610.3 Apis mellifera (honey bee) XP_001945506.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000044440 Bos taurus (bovine) CN09360 Caenorhabditis brenneri (small nematode) CBP31481 Caenorhabditis briggsae (nematode) CE39724 Caenorhabditis elegans (worm) ENSCAFP00000024261 Canis familiaris (dog) JA03585 Caenorhabditis japonica (nematode) ENSCPOP00000009409 Cavia porcellus (guinea pig) 220966 Capitella spl (polychaete worm) DDB0233315 Dictyostelium discoideum (amoeba) ENSECAP00000020390 Equus caballus (horse) ENSGACP00000025091 Gymnopilus aculeatus (mushroom) ENSGALP00000021624 Gallus gallus (chicken) 189723 Hypsipyla robusta (cedar shoot borer) ENSP00000380942 Homo sapiens (human) 171725 Lottia gigantea (owl limpet) ENSMODP00000016438 Monodelphis domestica (gray opossum) ENSMUSP00000072547 Mus musculus (house mouse) ENSOANP00000010025 Ornithorynchus anatinus (platypus) PP14797 Pristionchus pacificus (nematode) ENSPPYP00000004552 Pongo pygmaeus (orangutan) ENSPTRP00000058269 Pan troglodytes (chimpanzee) ENSRNOP00000056613 Rattus norvegicus (norway rat) Smp_126600 Schistosoma mansoni (trematode parasite) 62108 Trichoplax adhaerens (metazoa) ENSTNIP00000010605 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000029590 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000051338 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB18432 PB10144 NV21571 NV10482 LH16296 ISCW023724 ISCW023722 FBgn0229436 FBgn0223903 FBgn0206827 FBgn0182992 FBgn0176627 JGI_V11_52830 JGI_V11_104788 FBgn0245770 FBgn0144069 FBgn0130613 FBgn0112841 FBgn0088470 CPIJ004468 CPIJ017632 CPIJ017631 BGIBMGA008158 ACYPI002690 GB13906 AGAP010767 AAEL013102 AAEL011061 PHUM164820 PHUM165150 GLEAN_03070 GLEAN_03069
Stocks & Reagents
Stocks Listed in FlyBase ( 17 )
Bloomington	9386 y1 w*; P{UASp-T7.RhoGEF2}5 24032 w1118; Mi{ET1}ΡGEF2MB02788 9387 y1 w*; P{UASp-T7.RhoGEF2}6b 31239 y1 v1; P{TRiP.JF01747}attP2 18190 w1118; PBac{RB}RhoGEF2e03784/CyO 9388 y1 w*; P{UASp-T7.RhoGEF2.DeltaPDZ}4a 11369 cn1 P{PZ}RhoGEF204291/CyO; ry506 34643 y1 sc* v1; P{TRiP.HMS01118}attP2 20022 y1 w67c23; P{EPgy2}RhoGEF2EY08391 9122 y1 w*; P{FRT(whs)}G13 RhoGEF24.1/CyO 9382 w*; RhoGEF24.4/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7
Harvard	- PBac{RB}RhoGEF2e03784
	More stocks available...
Genomic Clones ( 2 )
	BACR09M08 BACR32P08 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 63 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	SD04476 SD10031
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM25011 HL03229 RH56752 SD06759 SD09362 SD13768 SD14264 SD22588
Other clones	29745 33119 41886 59621 61648 73424 102896 120827 144489 165508 214243.45 214373.3 214473.76 214501.27 214507.1 569814 CK01.9A.E8 CK01.9A.H7 CK01.29C.A6 CK01.40A.C10 CK01.66D.H2 CK01.114A.G8 CK01.123D.G10 CK01.145D.G7 CK01905 Dmel_dig_011886_0607_0858 Dmel_dig_021264_0608_0622 EC052052 EC054053 EC054547 EC055049 EC055491 EC055801 EC056006 EC056108 EC056380 EK004460 EK026135 EK050001 EK054061 EK119828 EK155046 EK187259 EK202446 EK250237 EK270062 EN15654 EP00155 EP05548 EP09014 EP14412 EP14805 EP19737
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0023172 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36915
Antibody Information
Other Information
Discoverer
Etymology
	Named "shar pei" after the breed of dog characterized by numerous skin folds, to reflect the phenotype of mutant embryos. (Settleman and Barrett, 1997.8.8)
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	RhoGEF2 is required for furrow canal formation during cellularisation. (Grosshans et al., 2005) RhoGEF2 activity is required for actin organization and acto-myosin contractility during somatic nuclear divisions, pole cell formation and cellularization of syncytial blastoderm embryos. (Padash Barmchi et al., 2005) RhoGEF2 is required for epithelial folding and invagination. (Nikolaidou, 2004) RhoGEF2 travels to the cell cortex on the tips of growing microtubules by interaction with the microtubule plus-end tracking protein Eb1. (Rogers et al., 2004) dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Mutations in RhoGEF2 disrupt the coordinates induction of cell shape changes. (Hacker and Perrimon, 1998) Rho1 is required for invagination of mesodermal and endodermal primordia during gastrulation and its activation is likely to be regulated by RhoGEF2. (Hacker and Perrimon, 1998) RhoGEF2 is essential for the invagination of mesodermal and endodermal primordia during gastrulation. (Hacker and Perrimon, 1998) RhoGEF2 is essential for directing the cell shape changes associated with gastrulation. (Barrett et al., 1997) Embryos lacking RhoGEF2 (generated as germline clones) show inappropriate lateral folds. (Settleman and Barrett, 1997.8.8) The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation. (Perrimon et al., 1996)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_057969 NM_206146 NM_206147 RefSeq (Proteins) • NP_477317 NP_995868 NP_995869 DDBJ / EMBL / GenBank • AAB88816 AAC38820 AAF57915 AAL39949 AAM68489 AAM68490 AC004335 AC005717 AF031930 AF032870 AI532843 AQ025625 AQ073581 AW944363 AY069804 CL610210 CZ481539 CZ481540 Entrez Gene - A searchable database of RefSeq genes. • 36915 UniProtKB/TrEMBL • A1ZAN6 A1ZAN7 O44113 O44381 Q8T9E3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dbl homology (DH) domain (IPR000219) PDZ/DHR/GLGF (IPR001478) Pleckstrin homology domain (IPR001849) Protein kinase C-like, phorbol ester/diacylglycerol binding (IPR002219) Pleckstrin homology-type (IPR011993) Regulator of G protein signalling-like fold (IPR015212) Rho GTP exchange factor (IPR015721) Regulator of G protein signalling superfamily (IPR016137)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62615 DroID - A comprehensive database of gene and protein interactions. • FBgn0023172 DRSC - Results from RNAi screens. • FBgn0023172 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023172 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9635-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0023172 FlyReactome - A curated knowledgebase of Drosophila melanogaster pathways • 351010 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36915 InParanoid A subset of ortholog calls from InParanoid. • AGAP010767-PA Anopheles gambiae (mosquito) XP_393610.3 Apis mellifera (honey bee) XP_001945506.1 Acyrthosiphon pisum (pea aphid) ENSBTAP00000044440 Bos taurus (bovine) CN09360 Caenorhabditis brenneri (small nematode) CBP31481 Caenorhabditis briggsae (nematode) CE39724 Caenorhabditis elegans (worm) ENSCAFP00000024261 Canis familiaris (dog) JA03585 Caenorhabditis japonica (nematode) ENSCPOP00000009409 Cavia porcellus (guinea pig) 220966 Capitella spl (polychaete worm) DDB0233315 Dictyostelium discoideum (amoeba) ENSECAP00000020390 Equus caballus (horse) ENSGACP00000025091 Gymnopilus aculeatus (mushroom) ENSGALP00000021624 Gallus gallus (chicken) 189723 Hypsipyla robusta (cedar shoot borer) ENSP00000380942 Homo sapiens (human) 171725 Lottia gigantea (owl limpet) ENSMODP00000016438 Monodelphis domestica (gray opossum) ENSMUSP00000072547 Mus musculus (house mouse) ENSOANP00000010025 Ornithorynchus anatinus (platypus) PP14797 Pristionchus pacificus (nematode) ENSPPYP00000004552 Pongo pygmaeus (orangutan) ENSPTRP00000058269 Pan troglodytes (chimpanzee) ENSRNOP00000056613 Rattus norvegicus (norway rat) Smp_126600 Schistosoma mansoni (trematode parasite) 62108 Trichoplax adhaerens (metazoa) ENSTNIP00000010605 Tetraodon nigroviridis (green spotted pufferfish) ENSTRUP00000029590 Takifugu rubripes (pufferfish Takifugu) ENSXETP00000051338 Xenopus tropicalis (frog) Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /cytoskel/rhogef2_1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36915 modMine - Data generated by the modENCODE project. • FBgn0023172 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • PB18432 PB10144 NV21571 NV10482 LH16296 ISCW023724 ISCW023722 FBgn0229436 FBgn0223903 FBgn0206827 FBgn0182992 FBgn0176627 JGI_V11_52830 JGI_V11_104788 FBgn0245770 FBgn0144069 FBgn0130613 FBgn0112841 FBgn0088470 CPIJ004468 CPIJ017632 CPIJ017631 BGIBMGA008158 ACYPI002690 GB13906 AGAP010767 AAEL013102 AAEL011061 PHUM164820 PHUM165150 GLEAN_03070 GLEAN_03069
Synonyms & Secondary IDs ( 24 )
Reported As
Symbol Synonym	CG9635 (Lee et al., 2003, Molnar et al., 2006, Ni et al., 2008, Ni et al., 2010.12.1, Keleman et al., 2009.8.5) DRhoGEF (Shook and Keller, 2003, Barrett et al., 1998) dRhoGEF (Rodriguez et al., 1999) DRhoGEF2 (Zhu et al., 2005, Nikolaidou, 2004, Nikolaidou, 2004, Rogers et al., 2004, Haecker et al., 2005, Gardano et al., 2005, Bayer et al., 2003, Settleman, 2001, Settleman, 2000, Nikolaidou and Barrett, 2002, Mizuno et al., 2002, Escott and Barrett, 2001, Sotillos and Campuzano, 2000, Grosshans and Wieschaus, 2000, Magie et al., 1999, Sutherland and Witke, 1999, Asha et al., 1999, Knust and Muller, 1998, Pourquie and Lemaire, 1998, Haecker and Perrimon, 1998, Hacker et al., 1998, Hacker and Perrimon, 1998, Barrett et al., 1997, Leptin, 2005, Mulinari et al., 2007, Marques et al., 2007, Padash Barmchi et al., 2005, Simoes et al., 2006, Mulinari et al., 2008, Mulinari et al., 2008, Mulinari and Häcker, 2009) dRhoGEF2 (Zheng et al., 1999, Brouzes and Farge, 2004) DrhoGEF2 (Chen et al., 2004, He and Adler, 2003, Bilder, 2001, Baer et al., 2007) Gef2 (Massarwa et al., 2009) l(2)04291 (Hacker and Perrimon, 1998, ) RG2 (Wenzl et al., 2010) RhoGEF (Ng and Luo, 2004, Akdemir et al., 2006) RhoGef2 (Martin, 2004) rhoGEF2 (Haecker, 1997.10.30) rhogef2 (Kolesnikov, 2006) RhoGEF2 (Webb et al., 2007, Wenzl and Grosshans, 2007, Fox et al., 2005, Jordan et al., 2005, Khoo et al., 2007, Wolfgang et al., 2007, Tountas and Fortini, 2007, Kolsch et al., 2007, Christensen et al., 2007.10.29, Christensen and Cook, 2007.10.29, Bakal et al., 2007, Fox and Peifer, 2007, Wenzl et al., 2010, Koelsch and Leptin, 2008, Molnar et al., 2006, Bakal et al., 2007, Cao et al., 2008, Ng, 2008, Kolesnikov and Beckendorf, 2007, Stofanko et al., 2008, Webb et al., 2009, Widmann and Dahmann, 2009, Homem and Peifer, 2008, Webb et al., 2009, Zeitlinger et al., 2007, Rehwinkel et al., 2005, Patch et al., 2009, Laplante and Nilson, 2011, Wasbrough et al., 2010) Rho-GEF2 (Corrigall et al., 2007, Yan et al., 2009) shar (Settleman and Barrett, 1997.8.8, ) Su(Rho1)2B (Barrett et al., 1997) T2 (Hacker and Perrimon, 1998) unnamed (Barrett and Settleman, 1997)
Name Synonym	akkordion   guanine nucleotide exchange factor 2 (Kolesnikov and Beckendorf, 2007) RhoGEF2 (Patch et al., 2009) Shar pei (Barrett, 1997.11.3, ) shar pei (Settleman and Barrett, 1997.8.8, ) Transcription unit 2 (Hacker and Perrimon, 1998)
Secondary FlyBase IDs
	FBgn0010569 FBgn0020519
References ( 125 )
Generate a list of	All references relating to this gene ( 125 )
List References by type	Research paper  ( 51 ) Supplementary material  ( 8 ) Review  ( 13 ) Personal communication to FlyBase  ( 14 ) Abstract  ( 26 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 2 ) Conference report  ( 2 ) Computer file  ( 4 ) Automatic genome annotation  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Laplante and Nilson, 2011, J. Cell Biol. 192(2): 335--348 Asymmetric distribution of Echinoid defines the epidermal leading edge during Drosophila dorsal closure. [FBrf0212838] Wenzl et al., 2010, Mech. Dev. 127(7-8): 371--384 Localization of RhoGEF2 during Drosophila cellularization is developmentally controlled by slam. [FBrf0211115] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...