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General Information
Symbol
Dmel\RhoGEF2
Species
D. melanogaster
Name
Rho guanine nucleotide exchange factor 2
Annotation Symbol
CG9635
Feature Type
FlyBase ID
FBgn0023172
Gene Model Status
Stock Availability
Gene Snapshot
Rho guanine nucleotide exchange factor 2 (RhoGEF2) encodes a protein involved in actin organization and contractility. It contributes to multiple actin-based processes including wound healing, cell shape and cell contraction. [Date last reviewed: 2019-08-01]
Also Known As

DRhoGEF2, RhoGEF, shar, DRhoGEF, shar pei

Key Links
Genomic Location
Cytogenetic map
Sequence location
2R:17,027,190..17,044,653 [+]
Recombination map

2-81

RefSeq locus
NT_033778 REGION:17027190..17044653
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (28 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity
Biological Process (20 terms)
Terms Based on Experimental Evidence (20 terms)
CV Term
Evidence
References
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:fog; FB:FBgn0000719
inferred from mutant phenotype
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (7 terms)
Terms Based on Experimental Evidence (7 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (0 terms)
Protein Family (UniProt)
-
Summaries
Gene Group (FlyBase)
RHO GUANINE NUCLEOTIDE EXCHANGE FACTORS -
RhoGEFs are Guanine Nucleotide Exchange Factors (GEFs) for Rho family GTPases. They include Dbl-homology domain-containing proteins (the largest family of RhoGEFs) and CZH2 domain/Dock family GEFs. (Adapted from FBrf0190957).
Summary (Interactive Fly)

induces contractile cell shape changes by stimulating myosin II via the Rho1 pathway - location at the actin cortex regulated by interaction with the microtubule plus-end tracking protein EB1

Gene Model and Products
Number of Transcripts
5
Number of Unique Polypeptides
4

Please see the GBrowse view of Dmel\RhoGEF2 or the JBrowse view of Dmel\RhoGEF2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0086971
8475
2559
FBtr0086969
7424
2205
FBtr0086970
8486
2559
FBtr0305083
8480
2557
FBtr0340124
8166
2456
Additional Transcript Data and Comments
Reported size (kB)

10.5, 8.5 (northern blot)

9.5 (northern blot)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0086125
280.8
2559
6.76
FBpp0086123
242.2
2205
6.37
FBpp0086124
280.8
2559
6.76
FBpp0293620
280.6
2557
6.76
FBpp0309117
269.6
2456
6.61
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

2559 aa isoforms: RhoGEF2-PD, RhoGEF2-PF
Additional Polypeptide Data and Comments
Reported size (kDa)

2559 (aa); 281 (kD predicted)

2559 (aa); 284 (kD predicted)

Comments
External Data
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\RhoGEF2 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

Expression in stage P3 pupal leg discs is restricted to presumptive tarsal joints.

The RhoGEF2 transcript is abundant in syncytial blastoderm embryos, continues to be detected during gastrulation, and is not detected after germ band extension.

Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

RhoGEF2 protein shows apical localization in trachea.

RhoGEF2 protein is enriched in groove founder cells at embryonic stage 12 at the sites where segmental boundaries will form.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\RhoGEF2 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 25 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 24 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of RhoGEF2
Transgenic constructs containing regulatory region of RhoGEF2
Deletions and Duplications ( 6 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
actin filament & embryonic/larval tracheal system, with Scer\GAL4btl.PS
pole cell & cytoskeleton | germ-line clone
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (26)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
10 of 15
No
Yes
10 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1  
1 of 15
No
No
1 of 15
No
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (23)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
11 of 15
Yes
Yes
9 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (21)
7 of 13
Yes
Yes
7 of 13
Yes
Yes
6 of 13
No
Yes
2 of 13
No
No
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (17)
6 of 12
Yes
Yes
5 of 12
No
Yes
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
1 of 12
No
Yes
Danio rerio (Zebrafish) (31)
10 of 15
Yes
Yes
8 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (7)
12 of 15
Yes
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (0)
No records found.
Saccharomyces cerevisiae (Brewer's yeast) (4)
2 of 15
Yes
No
2 of 15
Yes
No
1 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (4)
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
No
1 of 12
Yes
No
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG0919007S )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091501QZ )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Mayetiola destructor
Hessian fly
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Aedes aegypti
Yellow fever mosquito
Anopheles darlingi
American malaria mosquito
Anopheles gambiae
Malaria mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Culex quinquefasciatus
Southern house mosquito
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )
No non-Dipteran orthologies identified
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )
No non-Insect Arthropod orthologies identified
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )
No non-Arthropod Metazoa orthologies identified
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (5)
2 of 10
1 of 10
1 of 10
1 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 2 )
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Metabolic Pathways
External Data
Linkouts
Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2R
Recombination map

2-81

Cytogenetic map
Sequence location
2R:17,027,190..17,044,653 [+]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
53E4-53F1
Limits computationally determined from genome sequence between P{lacW}l(2)k11405k11405&P{EP}EP641 and P{lacW}l(2)k10815k10815
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
53F1-53F2
53F1--3
53F1-53F2
(determined by in situ hybridisation)
53F-53F
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Left of (cM)
Right of (cM)
Notes

Maps at 2-80 to 2-85.

Stocks and Reagents
Stocks (25)
Genomic Clones (24)
cDNA Clones (56)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
    Drosophila Genomics Resource Center cDNA clones

    For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

    cDNA Clones, End Sequenced (ESTs)
    RNAi and Array Information
    Linkouts
    DRSC - Results frm RNAi screens
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    Antibody Information
    Laboratory Generated Antibodies
     
    Commercially Available Antibodies
     
    Other Information
    Relationship to Other Genes
    Source for database identify of
    Source for database merge of
    Additional comments
    Other Comments

    RhoGEF2 is required for furrow canal formation during cellularisation.

    RhoGEF2 activity is required for actin organization and acto-myosin contractility during somatic nuclear divisions, pole cell formation and cellularization of syncytial blastoderm embryos.

    RhoGEF2 is required for epithelial folding and invagination.

    RhoGEF2 travels to the cell cortex on the tips of growing microtubules by interaction with the microtubule plus-end tracking protein Eb1.

    dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

    Rho1 is required for invagination of mesodermal and endodermal primordia during gastrulation and its activation is likely to be regulated by RhoGEF2.

    Mutations in RhoGEF2 disrupt the coordinates induction of cell shape changes.

    RhoGEF2 is essential for the invagination of mesodermal and endodermal primordia during gastrulation.

    RhoGEF2 is essential for directing the cell shape changes associated with gastrulation.

    Embryos lacking RhoGEF2 (generated as germline clones) show inappropriate lateral folds.

    The autosomal "FLP-DFS" technique (using the P{ovoD1-18} P{FRT(whs)} P{hsFLP} chromosomes) has been used to identify the specific maternal effect phenotype for the zygotic lethal mutation.

    Origin and Etymology
    Discoverer
    Etymology

    Named "shar pei" after the breed of dog characterized by numerous skin folds, to reflect the phenotype of mutant embryos.

    Identification
    External Crossreferences and Linkouts ( 71 )
    Sequence Crossreferences
    NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
    GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
    GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
    RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
    UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
    Other crossreferences
    Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
    Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
    Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
    Flygut - An atlas of the Drosophila adult midgut
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
    KEGG Genes - Molecular building blocks of life in the genomic space.
    modMine - A data warehouse for the modENCODE project
    SignaLink - A signaling pathway resource with multi-layered regulatory networks.
    Linkouts
    BioGRID - A database of protein and genetic interactions.
    DroID - A comprehensive database of gene and protein interactions.
    DRSC - Results frm RNAi screens
    FLIGHT - Cell culture data for RNAi and other high-throughput technologies
    FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
    FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
    FlyMine - An integrated database for Drosophila genomics
    Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    MIST (genetic) - An integrated Molecular Interaction Database
    MIST (protein-protein) - An integrated Molecular Interaction Database
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Synonyms and Secondary IDs (25)
    Reported As
    Symbol Synonym
    RhoGEF2
    (Sidor et al., 2020, Boucher et al., 2019, Deneke et al., 2019, Dent et al., 2019, Garcia De Las Bayonas et al., 2019, Gerritsma et al., 2019, Herrera-Perez and Kasza, 2019, Krueger et al., 2019, Meltzer et al., 2019, Ng, 2019.8.30, Ray et al., 2019, Cho et al., 2018, Izquierdo et al., 2018, Kale et al., 2018, Mortensen et al., 2018, Richardson and Portela, 2018, An et al., 2017, Gilmour et al., 2017, Nakamura et al., 2017, Clandinin and Owens, 2016-, Crocker et al., 2016, Das et al., 2016, Kerridge et al., 2016, Sarov et al., 2016, Urbansky et al., 2016, Hosono et al., 2015, Turkel et al., 2015, Acharya et al., 2014, Ashwal-Fluss et al., 2014, Eliazer et al., 2014, Jalvingh et al., 2014, Nie et al., 2014, Bulgakova et al., 2013, Das et al., 2013, Khoo et al., 2013, Kwon et al., 2013, Lee and Harris, 2013, Peters and Rogers, 2013, Sotillos et al., 2013, Spahn and Reuter, 2013, Zhang et al., 2013, Adamson and Lajeunesse, 2012, Crest et al., 2012, Howell et al., 2012, Izutsu et al., 2012, Lim and Thiery, 2012, Murray et al., 2012, Brumby et al., 2011, Friedman et al., 2011, Greenberg and Hatini, 2011, Laplante and Nilson, 2011, Levayer et al., 2011, Mathew et al., 2011, Muyskens and Guillemin, 2011, Sun et al., 2011, Wasbrough et al., 2010, Wenzl et al., 2010, Patch et al., 2009, Webb et al., 2009, Webb et al., 2009, Widmann and Dahmann, 2009, Cao et al., 2008, Homem and Peifer, 2008, Koelsch and Leptin, 2008, Ng, 2008, Stofanko et al., 2008, Bakal et al., 2007, Bakal et al., 2007, Christensen and Cook, 2007.10.29, Christensen et al., 2007.10.29, Fox and Peifer, 2007, Khoo et al., 2007, Kolesnikov and Beckendorf, 2007, Kolsch et al., 2007, Tountas and Fortini, 2007, Webb et al., 2007, Wenzl and Großhans, 2007, Wolfgang et al., 2007, Zeitlinger et al., 2007, Molnar et al., 2006, Burgler and Macdonald, 2005, Fox et al., 2005, Jordan et al., 2005, Rehwinkel et al., 2005)
    Name Synonyms
    Rho guanine nucleotide exchange factor 2
    Transcription unit 2
    akkordion
    guanine nucleotide exchange factor 2
    Secondary FlyBase IDs
    • FBgn0010569
    • FBgn0020519
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (216)