FB2022_02, released March 29, 2022

Gene: Dmel\EloB

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General Information
Symbol
Dmel\EloB
Species
D. melanogaster
Name
Elongin B
Annotation Symbol
CG4204
Feature Type
protein_coding_gene
FlyBase ID
FBgn0023212
Gene Model Status
Current
Stock Availability
8 publicly available
Gene Summary
Elongin B (EloB) encodes an essential protein for development that participates in the control of wing cell identity. EloB, EloC and EloA encode for subunits of the Elongin complex, which interacts in vivo with the chromatin factor encoded by corto. The proteins encoded by EloB and EloC form an adaptor subcomplex of a Cul2-RING ubiquitin ligase complex. [Date last reviewed: 2019-03-07] (FlyBase Gene Snapshot)
All Summaries
Key Links
Genomic Location
Cytogenetic map
92F2-92F2
Sequence location
Recombination map
3-69
RefSeq locus
NT_033777 REGION:20578222..20579850
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (9 terms)
Molecular Function (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Biological Process (6 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
involved_in imaginal disc-derived wing vein specification
inferred from mutant phenotype
(Rougeot et al., 2013)
inferred from genetic interaction with FLYBASE:corto; FB:FBgn0010313
(Rougeot et al., 2013)
involved_in larval somatic muscle development
inferred from expression pattern
(Dobi et al., 2014)
inferred from mutant phenotype
(Dobi et al., 2014)
involved_in negative regulation of receptor signaling pathway via JAK-STAT
inferred from mutant phenotype
(Stec et al., 2013)
involved_in positive regulation of proteasomal ubiquitin-dependent protein catabolic process
inferred from mutant phenotype
(Nayak et al., 2018)
involved_in viral process
inferred from mutant phenotype
(Nayak et al., 2018)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
involved_in transcription elongation from RNA polymerase II promoter
inferred from electronic annotation with InterPro:IPR039049
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
inferred by curator from GO:0070449
(Rougeot et al., 2013)
Cellular Component (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
part_of Cul2-RING ubiquitin ligase complex
(Nayak et al., 2018)
part_of elongin complex
inferred from direct assay
(Rougeot et al., 2013)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
part_of elongin complex
inferred from biological aspect of ancestor with PANTHER:PTN000997498
(assigned by GO_Central )
(Gaudet et al., 2011)
part_of VCB complex
inferred from biological aspect of ancestor with PANTHER:PTN000997498
(assigned by GO_Central )
(Gaudet et al., 2011)
Gene Group (FlyBase)
Pathway (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
Elongin B (EloB) encodes an essential protein for development that participates in the control of wing cell identity. EloB, EloC and EloA encode for subunits of the Elongin complex, which interacts in vivo with the chromatin factor encoded by corto. The proteins encoded by EloB and EloC form an adaptor subcomplex of a Cul2-RING ubiquitin ligase complex. [Date last reviewed: 2019-03-07]
Pathway (FlyBase)
Negative Regulators of JAK-STAT Signaling Pathway -
Negative regulators of JAK-STAT signaling down-regulate the pathway, attenuating transcriptional control by Stat92E.
Gene Group (FlyBase)
VCB COMPLEX -
VCB complex (Vhl-EloC-EloB) is a member of a family of ubiquitin ligases that share a Cullin-Rbx module. VCB complex is a multiprotein complex, which consist of large modular assemblies comprising a RING domain, a scaffolding Cullin subunit, one or more adaptor proteins, and a substrate-recognition subunit. (Adapted from FBrf0129709, FBrf0240293 and PMID:15601820).
ELONGIN COMPLEX -
Elongin complex is a transcription elongation factor that increases RNA polymerase II elongation rate by suppressing transient pausing. Elongin complex is a heterotrimer composed of the large transcriptionally active subunit elongin A and two regulatory subunits elongin B and C, which form a stable subcomplex that binds to subunit A. (Adapted from FBrf0223250 and FBrf0180540).
Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\EloB for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O44226)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
EloB-RA
FBtr0083947
NM_079692
1207
118
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
EloB-PA
FBpp0083354
13.3
118
4.10
NP_524416
AAG22163
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\EloB using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 5 of adulthood & male -- day 7 of adulthood & male
spermatozoon
(Dorus et al., 2006)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
part_of Cul2-RING ubiquitin ligase complex
(Nayak et al., 2018)
part_of elongin complex
inferred from direct assay
(Rougeot et al., 2013)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\EloB in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 2 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 6 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of EloB
Transgenic constructs containing regulatory region of EloB
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\ELOB
11 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (2)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Elob
12 of 15
Yes
Yes
Mmus\Elobl
4 of 15
No
Yes
Rattus norvegicus (Norway rat) (5)
Rnor\Elob
12 of 13
Yes
Yes
Rnor\RGD1565653
3 of 13
No
Yes
Rnor\LOC103691042
1 of 13
No
Yes
Rnor\RGD1304870
1 of 13
No
Yes
Rnor\RGD1561118
1 of 13
No
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\elob
8 of 12
Yes
Yes
Danio rerio (Zebrafish) (2)
Drer\elob
7 of 15
Yes
Yes
Drer\LOC569097
2 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (1)
Cele\elb-1
12 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\AT5G57860
2 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Other Organism Orthologs (via OrthoDB)
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (0)
No records found.
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    EloBGD4863
    exacerbates  tauopathy
    modeled by Hsap\MAPTR406W.UAS
    (Butzlaff et al., 2015)
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    ELOB; elongin B
    11 of 15
    600787
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        EloB - Art8
        experimental knowledge based
        (Guruharsha et al., 2011)
        EloB - CG12081
        experimental knowledge based
        (Guruharsha et al., 2011)
        EloB - EloC
        anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based
        (Rougeot et al., 2013, Guruharsha et al., 2011)
        EloB - corto
        anti tag coimmunoprecipitation, anti tag western blot
        (Rougeot et al., 2013)
        Summary of Genetic Interactions
        esyN Network Diagram
        esyN Network Key:
        Suppression
        Enhancement
        View in Interactions Browser
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        corto
        suppressible
        EloB
        (Rougeot et al., 2013)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Negative Regulators of JAK-STAT Signaling Pathway -
        Negative regulators of JAK-STAT signaling down-regulate the pathway, attenuating transcriptional control by Stat92E.
        Metabolic Pathways
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        External Data
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-69
        Cytogenetic map
        92F2-92F2
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        92F2-92F2
        Limits computationally determined from genome sequence between P{PZ}l(3)1058510585 and P{EP}SyndEP409
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-69

        (FlyBase, 2016)

        3-72.8

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (8)
        Genomic Clones (15)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (90)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of

        Source for identity of: EloB Elongin-B

        (Rougeot et al., 2013)
        Source for database merge of

        Source for merge of: Elongin-B CG4204

        (Bayraktaroglu, 2000.4.12)
        Additional comments
        Other Comments

        The Elongin complex (comprising EloA, EloB and EloC) appears to participate in vein vs intervein specification in the wing, antagonising corto.

        (Rougeot et al., 2013)

        Area matching Drosophila Elongin B gene (inverted), Acc. No. AB007692.

        (Davies, 2001.3.15)

        Isolated from a Drosophila embryonic yeast two-hybrid cDNA library, using human Elongin B as a bait.

        (Aso and Conrad, 1997)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 46 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
        EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        MARRVEL_MODEL - MARRVEL (model organism gene)
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
        Synonyms and Secondary IDs (8)
        Datasets (8)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        Study result (8)
        Result
        Result Type
        Title
        scRNAseq_2020_Cattenoz_NI_seq_clustering
        Clustering analysis of hemocytes from non-infested third instar larvae
        scRNAseq_2020_Cattenoz_WI_seq_clustering
        Clustering analysis of hemocytes from wasp-infested third instar larvae
        scRNAseq_2020_Cho_NI72LG_seq_clustering
        Clustering analysis of lymph gland cells from non-infested larvae at 72 h after egg-laying
        scRNAseq_2020_Cho_NI96LG_seq_clustering
        Clustering analysis of lymph gland cells from non-infested larvae at 96 h after egg-laying
        scRNAseq_2020_Cho_NI120LG_seq_clustering
        Clustering analysis of lymph gland cells from non-infested larvae at 120 h after egg-laying
        scRNAseq_2020_Cho_WI96LG_seq_clustering
        Clustering analysis of lymph gland cells from wasp-infested larvae at 96 h after egg-laying
        scRNAseq_2020_Cho_NI96HL_seq_clustering
        Clustering analysis of circulating hemocytes from non-infested larvae at 96 h after egg-laying
        scRNAseq_2020_Cho_NI120HL_seq_clustering
        Clustering analysis of circulating hemocytes from non-infested larvae at 120 h after egg-laying
        References (50)