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General Information
Symbol
Dmel\eIF4G1
Species
D. melanogaster
Name
eukaryotic translation initiation factor 4G1
Annotation Symbol
CG10811
Feature Type
FlyBase ID
FBgn0023213
Gene Model Status
Stock Availability
Gene Summary
eukaryotic translation initiation factor 4G1 (eIF4G1) encodes a protein that contributes to translation and spermatogenesis. [Date last reviewed: 2019-07-11] (FlyBase Gene Snapshot)
Also Known As

eIF4G, eIF-4G, eukaryotic translation initiation factor 4G, Eukaryotic-initiation-factor-4G, deIF4G

Key Links
Genomic Location
Cytogenetic map
Sequence location
4:915,297..930,715 [-]
Recombination map
4-0
RefSeq locus
NC_004353 REGION:915297..930715
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (5 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
inferred from physical interaction with FLYBASE:eIF4E4; FB:FBgn0035709
inferred from physical interaction with FLYBASE:eIF4E5; FB:FBgn0035823
inferred from physical interaction with FLYBASE:eIF4E7; FB:FBgn0040368
inferred from physical interaction with FLYBASE:eIF4E3; FB:FBgn0265089
enables RNA binding
inferred from direct assay
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables mRNA binding
inferred from biological aspect of ancestor with PANTHER:PTN000583430
(assigned by GO_Central )
inferred from sequence or structural similarity with HGNC:3296,SGD:S000003394
inferred from biological aspect of ancestor with PANTHER:PTN000583430
(assigned by GO_Central )
inferred from sequence or structural similarity
Biological Process (4 terms)
Terms Based on Experimental Evidence (3 terms)
CV Term
Evidence
References
involved_in mitotic cell cycle
inferred from high throughput mutant phenotype
inferred from mutant phenotype
involved_in translation
inferred from direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from sequence or structural similarity
inferred from sequence or structural similarity with HGNC:3296,SGD:S000003394
Cellular Component (2 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000583430
(assigned by GO_Central )
Protein Family (UniProt)
-
Summaries
Gene Snapshot
eukaryotic translation initiation factor 4G1 (eIF4G1) encodes a protein that contributes to translation and spermatogenesis. [Date last reviewed: 2019-07-11]
Gene Group (FlyBase)
CYTOPLASMIC TRANSLATION INITIATION FACTORS -
Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\eIF4G1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.47

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0089243
5576
1666
FBtr0112904
6335
1919
FBtr0289951
5806
1666
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
FBpp0088303
183.9
1666
7.92
FBpp0111817
211.1
1919
8.54
FBpp0288389
183.9
1666
7.92
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1666 aa isoforms: eIF4G1-PA, eIF4G1-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF4G1 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
organism

Comment: maternally deposited

Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
Additional Descriptive Data

eIF4G1 protein is expressed primarily in somatic cyst cells. eIF4G1 protein can also be detected at lower levels in germ cells at some stages of spermatogeneis; it is localized to the cytoplasm of spermatogonia and primary spermatocytes, but is excluded from meiotic and post-meiotic stage germ cells.

Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytosol
inferred from high throughput direct assay
Expression Deduced from Reporters
Stage
Tissue/Position (including subcellular localization)
Reference
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF4G1 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
EMBL-EBI Single Cell Expression Atlas
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 4 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of eIF4G1
Transgenic constructs containing regulatory region of eIF4G1
Aberrations (Deficiencies and Duplications) ( 1 )
Inferred from experimentation ( 1 )
Gene disrupted in
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
11 of 15
Yes
Yes
3 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (4)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
11 of 15
Yes
Yes
10 of 15
No
Yes
3 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (6)
9 of 13
Yes
Yes
5 of 13
No
Yes
4 of 13
No
Yes
2 of 13
No
No
2 of 13
No
Yes
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (4)
5 of 12
Yes
Yes
5 of 12
Yes
Yes
2 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (8)
9 of 15
Yes
Yes
7 of 15
No
Yes
7 of 15
No
Yes
3 of 15
No
No
3 of 15
No
No
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
Caenorhabditis elegans (Nematode, roundworm) (1)
9 of 15
Yes
Yes
Arabidopsis thaliana (thale-cress) (5)
5 of 9
Yes
Yes
2 of 9
No
No
2 of 9
No
No
1 of 9
No
Yes
1 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (2)
7 of 15
Yes
Yes
6 of 15
No
Yes
Schizosaccharomyces pombe (Fission yeast) (1)
6 of 12
Yes
Yes
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091900GK )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Drosophila simulans
Drosophila sechellia
Drosophila erecta
Drosophila yakuba
Drosophila ananassae
Drosophila pseudoobscura pseudoobscura
Drosophila persimilis
Drosophila willistoni
Drosophila virilis
Drosophila mojavensis
Drosophila grimshawi
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500J2 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Glossina morsitans
Tsetse fly
Lucilia cuprina
Australian sheep blowfly
Mayetiola destructor
Hessian fly
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00R3 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Danaus plexippus
Monarch butterfly
Heliconius melpomene
Postman butterfly
Apis florea
Little honeybee
Apis mellifera
Western honey bee
Bombus impatiens
Common eastern bumble bee
Bombus terrestris
Buff-tailed bumblebee
Linepithema humile
Argentine ant
Megachile rotundata
Alfalfa leafcutting bee
Nasonia vitripennis
Parasitic wasp
Nasonia vitripennis
Parasitic wasp
Dendroctonus ponderosae
Mountain pine beetle
Tribolium castaneum
Red flour beetle
Pediculus humanus
Human body louse
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Rhodnius prolixus
Kissing bug
Cimex lectularius
Bed bug
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Acyrthosiphon pisum
Pea aphid
Zootermopsis nevadensis
Nevada dampwood termite
Zootermopsis nevadensis
Nevada dampwood termite
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00PN )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Ixodes scapularis
Black-legged tick
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Stegodyphus mimosarum
African social velvet spider
Tetranychus urticae
Two-spotted spider mite
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Daphnia pulex
Water flea
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G03ZW )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Strongylocentrotus purpuratus
Purple sea urchin
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Ciona intestinalis
Vase tunicate
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Gallus gallus
Domestic chicken
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (3)
5 of 10
4 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
RNA-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Genomic Location and Detailed Mapping Data
Chromosome (arm)
4
Recombination map
4-0
Cytogenetic map
Sequence location
4:915,297..930,715 [-]
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
102F1-102F1
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
102E-102E
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (8)
Genomic Clones (13)
 

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (119)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
Commercially Available Antibodies
 
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: eIF4G eIF-4G

Source for identity of: eIF4G1 eIF4G

Source for database merge of
Additional comments
Other Comments

Candidate stable intronic sequence RNA (sisRNA) identified within CDS of this gene.

RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA.

eIF-4G has been cloned and sequenced.

Drosophila initiation factor eIF-4F is composed of two subunits, one is encoded by eIF-4E and the other by eIF-4G, a 200kD protein. eIF-4G is required for the translation of endogenous mRNAs in cell-free systems made from Drosophila embryos, and can cross-link uncapped, but not m7G capped RNA. eIF-4E cross-links with m7G capped RNA.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 58 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
Other crossreferences
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
EMBL-EBI Single Cell Expression Atlas
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL
modMine - A data warehouse for the modENCODE project
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (19)
Reported As
Symbol Synonym
Eif4G
eIF4G
(Dold et al., 2020, Lasko, 2020, Vandehoef et al., 2020, Vasudevan et al., 2020, Laflamme et al., 2017, Ma et al., 2017, Rissland et al., 2017, Transgenic RNAi Project members, 2017-, Wang et al., 2017, Grüner et al., 2016, Na et al., 2016, Vinayagam et al., 2016, Zhang et al., 2016, Zuberek et al., 2016, Dent et al., 2015, Gehrke et al., 2015, Ghosh and Lasko, 2015, Horan et al., 2015, Liu et al., 2015, Pek et al., 2015, Peter et al., 2015, Sopko et al., 2015, Vanhauwaert and Verstreken, 2015, Ashwal-Fluss et al., 2014, Ghosh et al., 2014, Igreja et al., 2014, Cui et al., 2013, Hernández et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Kwon et al., 2013, Yoshigi et al., 2013, Zekri et al., 2013, Gonsalvez and Long, 2012, Hernandez et al., 2012, Jansen and Niessing, 2012, Killip and Grewal, 2012, Mihailovich et al., 2012, Vazquez-Pianzola and Suter, 2012, Friedman et al., 2011, Garrey et al., 2010, Jäger and Dorner, 2010, Müller et al., 2010, Papoulas et al., 2010, Wasbrough et al., 2010, Duncan et al., 2009, Iwasaki et al., 2009, Pisa et al., 2009, Zekri et al., 2009, Duncan, 2008, Hernandez et al., 2008, Baker and Fuller, 2007, Franklin-Dumont et al., 2007, Franklin-Dumont et al., 2007, Fuller and Baker, 2007.6.28, Baker and Fuller, 2006, Hernandez and Vazquez-Pianzola, 2005, Hernandez et al., 2005, Macdonald, 2005, Hernandez et al., 2004, Kozak, 2004, Macdonald, 2004, Nelson et al., 2004, Zappavigna et al., 2004, Gamberi et al., 2003, Herold et al., 2003, Herold et al., 2001, Johnstone and Lasko, 2001, Lasko, 2000, Locke et al., 2000, Lehner, 1999, Hernandez et al., 1998, Zapata et al., 1994)
Name Synonyms
Eukaryotic initiation factor 4G
Eukaryotic-initiation-factor-4G
eukaryotic initiation factor 4G
eukaryotic translation initiation factor 4G
eukaryotic translation initiation factor 4G1
translation initiation factor
translation initiation factor 4G
translation initiation factor eIF4G
Secondary FlyBase IDs
    Datasets (0)
    Study focus (0)
    Experimental Role
    Project
    Project Type
    Title
    References (147)