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Gene Dmel\eIF4G

General Information
Symbol	Dmel\eIF4G	Species	D. melanogaster
Name	eukaryotic translation initiation factor 4G	Annotation symbol	CG10811
Feature type	protein_coding_gene	FlyBase ID	FBgn0023213
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	eIF-4G, deIF4G
Genomic Location
Chromosome (arm)	4	Recombination map
Cytogenetic map	102F1-102F1	Sequence location	4:935,923..951,341 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene eukaryotic translation initiation factor 4G is referred to in FlyBase by the symbol Dmel\eIF4G (CG10811, FBgn0023213). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: eukaryotic initiation factor 4E binding; RNA binding; RNA 7-methylguanosine cap binding. There is experimental evidence that it is involved in the biological process: mitotic spindle elongation; G1/S transition of mitotic cell cycle; mitotic G2 DNA damage checkpoint; mitosis; mitotic spindle organization; translation. 5 alleles are reported. No phenotypic data is available. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Armadillo-type fold; Initiation factor eIF-4 gamma, MA3; MIF4-like, type 1/2/3; MIF4G-like, type 3; W2 domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. Comments on Affy2 ProbeSet: ProbeSet 1637560_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of eIF4G. Gene sequence location is 4:935923..951341.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 102F1-102F1
Experimentally Determined Cytological Location
Cytogenetic map Notes References 102E-102E     (Hernandez, 1997.10.16) 102E-102E   (determined by in situ hybridisation)   (Hernandez et al., 1998)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\eIF4G for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Low-frequency RNA-Seq exon junction(s) not annotated. Gene model reviewed during 5.47
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) eIF4G-RA FBtr0089243  NM_079901   5576   1666 eIF4G-RB FBtr0112904  NM_001103382   6335   1919 eIF4G-RC FBtr0289951  NM_001144385   5806   1666
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein eIF4G-PA   FBpp0088303   183.9   1666   7.92     NP_524640   AAF59403 eIF4G-PB   FBpp0111817   211.1   1919   8.54     NP_001096852   ABV53593 eIF4G-PC   FBpp0288389   183.9   1666   7.92     NP_001137857   ACL82868
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • W2 domain (IPR003307) MIF4G-like, type 3 (IPR003890) Initiation factor eIF-4 gamma, MA3 (IPR003891) MIF4-like, type 1/2/3 (IPR016021) Armadillo-type fold (IPR016024)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA735894   AE014135 AAF59403   AE014135 ABV53593   AC010916   AE014135 ACL82868   AF030155 AAC38985   AI295591   AW941972   BG637151   BI229842   BI235689   BI366194   BP542339   BP548011   BP549883   BP549884   BP552478   BP552479   BP553724   BP553725   BT009987 AAQ22456   BT053671   CA805303   CK661008   CO178792   CO178912   CO182949   CO184064   CO186534   CO187915   CO188382   CO190349   CO192894   CO261905   CO263104   CO263765   CO264851   CO267695   CO269089   CO269265   CO273249   CO278211   CO279641   CO281608   CO282397   CO285416   CO287359   CO287474   CO290304   CO291109   CO294285   CO297305   CO302755   CO303309   CO303403   CO304565   CO306233   CO306855   CO306930   CO308228   CO309307   CO311613   CO314437   CO320772   CO322397   CO327697   CO328346   CO330201   CO339494   CO345486   CZ479912   EC058406   EC058872   EC059231   EC066920   EC067224   EC074523   EC075851   EC080462   EC081197   EC081975   EC082282   EC089081   EC090288   EC196944   EC197227   EC198639   EC199531   EC201245   EC203148   EC208156   EC209624   EC214195   EC214675   EC219574   EC223065   EC225305   EC226032   EC229933   EC234655   EC236318   EC237389   EC240085   EC243130   EC244345   EC245081   EC249304   EC250251   EC250610   EC251532   EC257586   EC258826   EV580325   EV586820   EV590578   EV593167   EV593938   EV596004   EV596963   EV598466   GH941161   GH944501   GH945028   GH946017   GH949782   GH950198   GH950740   GH953855   GH954438   GH961320   GH961847   GH962752   GH964955   GH967827   GH971174   GH976249   GH982491   GH983019   GR705741
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O61380 A8DZ29 Q7YU50
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | faint & ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy Stage Tissue/Position (including subcellular localization) Reference day 5 of adulthood & male -- day 7 of adulthood & male   spermatozoon   (Wasbrough et al., 2010)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
eukaryotic translation initiation factor 4F complex inferred from direct assay (Zapata et al., 1994)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval/adult carcass. Expression at moderate levels in the following post-embryonic organs or tissues: adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1241.025 Larval Midgut    1285.6 Larval Hindgut    1156.4 Larval Malpighian Tubules    1565.6 Larval Fat Body    814.3 Larval Salivary Gland    2815.5 Larval Trachea    1059.6 Larval Carcass    1404.35 Adult Head    568.9 Adult Eye    534.85 Adult Brain    807 Adult Thoracic-Abdominal Ganglion    672.8 Adult Crop    868.8 Adult Midgut    942 Adult Hindgut    677.6 Adult Malpighian Tubules    1909 Adult Fat Body    1554.8 Adult Salivary Gland    1581 Adult Heart    998.95 Adult VirginFemale Spermatheca    1788.4 Adult InseminatedFemale Spermatheca    2122.8 Adult Ovary    1176.5 Adult Testis    162.4 Adult Male Accessory Gland    1714.4 Adult Carcass    741.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1241.025) Larval Midgut  (1285.6) Larval Hindgut  (1156.4) Larval Malpighian Tubules  (1565.6) Larval Fat Body  (814.3) Larval Salivary Gland  (2815.5) Larval Trachea  (1059.6) Larval Carcass  (1404.35) Adult Head  (568.9) Adult Eye  534.85 Adult Brain  (807) Adult Thoracic-Abdominal Ganglion  (672.8) Adult Crop  (868.8) Adult Midgut  (942) Adult Hindgut  (677.6) Adult Malpighian Tubules  (1909) Adult Fat Body  (1554.8) Adult Salivary Gland  (1581) Adult Heart  (998.95) Adult VirginFemale Spermatheca  (1788.4) Adult InseminatedFemale Spermatheca  (2122.8) Adult Ovary  (1176.5) Adult Testis    162.4 Adult Male Accessory Gland  (1714.4) Adult Carcass  (741.1) Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  (1241.025) Larval Midgut  (1285.6) Larval Hindgut  (1156.4) Larval Malpighian Tubules  (1565.6) Larval Fat Body    814.3 Larval Salivary Gland  (2815.5) Larval Trachea  1059.6 Larval Carcass  (1404.35) Adult Head    568.9 Adult Eye    534.85 Adult Brain    807 Adult Thoracic-Abdominal Ganglion    672.8 Adult Crop    868.8 Adult Midgut    942 Adult Hindgut    677.6 Adult Malpighian Tubules  (1909) Adult Fat Body  (1554.8) Adult Salivary Gland  (1581) Adult Heart    998.95 Adult VirginFemale Spermatheca  (1788.4) Adult InseminatedFemale Spermatheca  (2122.8) Adult Ovary  (1176.5) Adult Testis    162.4 Adult Male Accessory Gland  (1714.4) Adult Carcass    741.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1241.025 Larval Midgut    1285.6 Larval Hindgut    1156.4 Larval Malpighian Tubules    1565.6 Larval Fat Body    814.3 Larval Salivary Gland    2815.5 Larval Trachea    1059.6 Larval Carcass    1404.35 Adult Head    568.9 Adult Eye    534.85 Adult Brain    807 Adult Thoracic-Abdominal Ganglion    672.8 Adult Crop    868.8 Adult Midgut    942 Adult Hindgut    677.6 Adult Malpighian Tubules    1909 Adult Fat Body    1554.8 Adult Salivary Gland    1581 Adult Heart    998.95 Adult VirginFemale Spermatheca    1788.4 Adult InseminatedFemale Spermatheca    2122.8 Adult Ovary    1176.5 Adult Testis    162.4 Adult Male Accessory Gland    1714.4 Adult Carcass    741.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    1241.025 Larval Midgut    1285.6 Larval Hindgut    1156.4 Larval Malpighian Tubules    1565.6 Larval Fat Body    814.3 Larval Salivary Gland    2815.5 Larval Trachea    1059.6 Larval Carcass    1404.35 Adult Head    568.9 Adult Eye    534.85 Adult Brain    807 Adult Thoracic-Abdominal Ganglion    672.8 Adult Crop    868.8 Adult Midgut    942 Adult Hindgut    677.6 Adult Malpighian Tubules    1909 Adult Fat Body    1554.8 Adult Salivary Gland    1581 Adult Heart    998.95 Adult VirginFemale Spermatheca    1788.4 Adult InseminatedFemale Spermatheca    2122.8 Adult Ovary    1176.5 Adult Testis    162.4 Adult Male Accessory Gland    1714.4 Adult Carcass    741.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (1241.025) Larval Midgut  (1285.6) Larval Hindgut  (1156.4) Larval Malpighian Tubules  (1565.6) Larval Fat Body  (814.3) Larval Salivary Gland  (2815.5) Larval Trachea  (1059.6) Larval Carcass  (1404.35) Adult Head  568.9 Adult Eye  534.85 Adult Brain  807 Adult Thoracic-Abdominal Ganglion  672.8 Adult Crop  (868.8) Adult Midgut  (942) Adult Hindgut  677.6 Adult Malpighian Tubules  (1909) Adult Fat Body  (1554.8) Adult Salivary Gland  (1581) Adult Heart  (998.95) Adult VirginFemale Spermatheca  (1788.4) Adult InseminatedFemale Spermatheca  (2122.8) Adult Ovary  (1176.5) Adult Testis    162.4 Adult Male Accessory Gland  (1714.4) Adult Carcass  741.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System  1241.025 Larval Midgut  1285.6 Larval Hindgut  1156.4 Larval Malpighian Tubules  1565.6 Larval Fat Body    814.3 Larval Salivary Gland  (2815.5) Larval Trachea  1059.6 Larval Carcass  1404.35 Adult Head    568.9 Adult Eye    534.85 Adult Brain    807 Adult Thoracic-Abdominal Ganglion    672.8 Adult Crop    868.8 Adult Midgut    942 Adult Hindgut    677.6 Adult Malpighian Tubules  (1909) Adult Fat Body  1554.8 Adult Salivary Gland  1581 Adult Heart    998.95 Adult VirginFemale Spermatheca  (1788.4) Adult InseminatedFemale Spermatheca  (2122.8) Adult Ovary  1176.5 Adult Testis    162.4 Adult Male Accessory Gland  1714.4 Adult Carcass    741.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    1241.025 Larval Midgut    1285.6 Larval Hindgut    1156.4 Larval Malpighian Tubules    1565.6 Larval Fat Body    814.3 Larval Salivary Gland    2815.5 Larval Trachea    1059.6 Larval Carcass    1404.35 Adult Head    568.9 Adult Eye    534.85 Adult Brain    807 Adult Thoracic-Abdominal Ganglion    672.8 Adult Crop    868.8 Adult Midgut    942 Adult Hindgut    677.6 Adult Malpighian Tubules    1909 Adult Fat Body    1554.8 Adult Salivary Gland    1581 Adult Heart    998.95 Adult VirginFemale Spermatheca    1788.4 Adult InseminatedFemale Spermatheca    2122.8 Adult Ovary    1176.5 Adult Testis    162.4 Adult Male Accessory Gland    1714.4 Adult Carcass    741.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    568.9   NA  Eye    534.85   NA  Brain    807   1241.025  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    672.8   NA  Crop    868.8   1285.6  Midgut    942   1156.4  Hindgut    677.6   1565.6  Malpighian Tubules    1909   814.3  Fat Body    1554.8   2815.5  Salivary Gland    1581   NA  Heart    998.95   1059.6  Trachea    NA   NA  VirginFemale Spermatheca    1788.4   NA  InseminatedFemale Spermatheca    2122.8   NA  Ovary    1176.5   NA  Testis    162.4   NA  Male Accessory Gland    1714.4   1404.35  Carcass    741.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    60 central nervous system, larvae L3    89 central nervous system, pupae P8    66 head, virgin 1-day female    65 head, virgin 4-day female    40 head, virgin 20-day female    34 head, mated 1-day female    40 head, mated 4-day female    42 head, mated 20-day female    37 head, mated 1-day male    48 head, mated 4-day male    47 head, mated 20-day male    52 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    59 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    55 digestive system, 4-day adult    74 digestive system, 20-day adult    47 fat body, larvae L3 wandering    33 fat body, white prepupae    62 fat body, pupae P8    17 carcass, larvae L3 wandering    80 carcass, 1-day adult    62 carcass, 4-day adult    72 carcass, 20-day adult    49 ovary, virgin 4-day female    151 ovary, mated 4-day female    117 testis, mated 4-day male    7 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (60) central nervous system, larvae L3  (89) central nervous system, pupae P8  (66) head, virgin 1-day female  (65) head, virgin 4-day female  (40) head, virgin 20-day female  (34) head, mated 1-day female  (40) head, mated 4-day female  (42) head, mated 20-day female  (37) head, mated 1-day male  (48) head, mated 4-day male  (47) head, mated 20-day male  (52) salivary gland, larvae L3 wandering  (34) salivary gland, white prepupae  (59) digestive system, larvae L3 wandering  (63) digestive system, 1-day adult  (55) digestive system, 4-day adult  (74) digestive system, 20-day adult  (47) fat body, larvae L3 wandering  (33) fat body, white prepupae  (62) fat body, pupae P8    17 carcass, larvae L3 wandering  (80) carcass, 1-day adult  (62) carcass, 4-day adult  (72) carcass, 20-day adult  (49) ovary, virgin 4-day female  (151) ovary, mated 4-day female  (117) testis, mated 4-day male    7 accessory gland, mated 4-day male  (32) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    60 central nervous system, larvae L3    89 central nervous system, pupae P8    66 head, virgin 1-day female    65 head, virgin 4-day female    40 head, virgin 20-day female    34 head, mated 1-day female    40 head, mated 4-day female    42 head, mated 20-day female    37 head, mated 1-day male    48 head, mated 4-day male    47 head, mated 20-day male    52 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    59 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    55 digestive system, 4-day adult    74 digestive system, 20-day adult    47 fat body, larvae L3 wandering    33 fat body, white prepupae    62 fat body, pupae P8    17 carcass, larvae L3 wandering    80 carcass, 1-day adult    62 carcass, 4-day adult    72 carcass, 20-day adult    49 ovary, virgin 4-day female  (151) ovary, mated 4-day female  (117) testis, mated 4-day male    7 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    60 central nervous system, larvae L3    89 central nervous system, pupae P8    66 head, virgin 1-day female    65 head, virgin 4-day female    40 head, virgin 20-day female    34 head, mated 1-day female    40 head, mated 4-day female    42 head, mated 20-day female    37 head, mated 1-day male    48 head, mated 4-day male    47 head, mated 20-day male    52 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    59 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    55 digestive system, 4-day adult    74 digestive system, 20-day adult    47 fat body, larvae L3 wandering    33 fat body, white prepupae    62 fat body, pupae P8    17 carcass, larvae L3 wandering    80 carcass, 1-day adult    62 carcass, 4-day adult    72 carcass, 20-day adult    49 ovary, virgin 4-day female    151 ovary, mated 4-day female    117 testis, mated 4-day male    7 accessory gland, mated 4-day male    32 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    60 central nervous system, larvae L3    89 central nervous system, pupae P8    66 head, virgin 1-day female    65 head, virgin 4-day female    40 head, virgin 20-day female    34 head, mated 1-day female    40 head, mated 4-day female    42 head, mated 20-day female    37 head, mated 1-day male    48 head, mated 4-day male    47 head, mated 20-day male    52 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    59 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    55 digestive system, 4-day adult    74 digestive system, 20-day adult    47 fat body, larvae L3 wandering    33 fat body, white prepupae    62 fat body, pupae P8    17 carcass, larvae L3 wandering    80 carcass, 1-day adult    62 carcass, 4-day adult    72 carcass, 20-day adult    49 ovary, virgin 4-day female    151 ovary, mated 4-day female    117 testis, mated 4-day male    7 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (60) central nervous system, larvae L3  (89) central nervous system, pupae P8  (66) head, virgin 1-day female  (65) head, virgin 4-day female  (40) head, virgin 20-day female  (34) head, mated 1-day female  (40) head, mated 4-day female  (42) head, mated 20-day female  (37) head, mated 1-day male  (48) head, mated 4-day male  (47) head, mated 20-day male  (52) salivary gland, larvae L3 wandering  (34) salivary gland, white prepupae  (59) digestive system, larvae L3 wandering  (63) digestive system, 1-day adult  (55) digestive system, 4-day adult  (74) digestive system, 20-day adult  (47) fat body, larvae L3 wandering  (33) fat body, white prepupae  (62) fat body, pupae P8    17 carcass, larvae L3 wandering  (80) carcass, 1-day adult  (62) carcass, 4-day adult  (72) carcass, 20-day adult  (49) ovary, virgin 4-day female  (151) ovary, mated 4-day female  (117) testis, mated 4-day male    7 accessory gland, mated 4-day male  32 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    60 central nervous system, larvae L3    89 central nervous system, pupae P8    66 head, virgin 1-day female    65 head, virgin 4-day female    40 head, virgin 20-day female    34 head, mated 1-day female    40 head, mated 4-day female    42 head, mated 20-day female    37 head, mated 1-day male    48 head, mated 4-day male    47 head, mated 20-day male    52 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    59 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    55 digestive system, 4-day adult    74 digestive system, 20-day adult    47 fat body, larvae L3 wandering    33 fat body, white prepupae    62 fat body, pupae P8    17 carcass, larvae L3 wandering    80 carcass, 1-day adult    62 carcass, 4-day adult    72 carcass, 20-day adult    49 ovary, virgin 4-day female  (151) ovary, mated 4-day female  117 testis, mated 4-day male    7 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    60 central nervous system, larvae L3    89 central nervous system, pupae P8    66 head, virgin 1-day female    65 head, virgin 4-day female    40 head, virgin 20-day female    34 head, mated 1-day female    40 head, mated 4-day female    42 head, mated 20-day female    37 head, mated 1-day male    48 head, mated 4-day male    47 head, mated 20-day male    52 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    59 digestive system, larvae L3 wandering    63 digestive system, 1-day adult    55 digestive system, 4-day adult    74 digestive system, 20-day adult    47 fat body, larvae L3 wandering    33 fat body, white prepupae    62 fat body, pupae P8    17 carcass, larvae L3 wandering    80 carcass, 1-day adult    62 carcass, 4-day adult    72 carcass, 20-day adult    49 ovary, virgin 4-day female    151 ovary, mated 4-day female    117 testis, mated 4-day male    7 accessory gland, mated 4-day male    32 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 06-24 hour embryonic stages, at stages throughout the larval period, at stages throughout the pupal period, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    26 embryo 04-06hr    53 embryo 06-08hr    56 embryo 08-10hr    65 embryo 10-12hr    48 embryo 12-14hr    93 embryo 14-16hr    39 embryo 16-18hr    54 embryo 18-20hr    34 embryo 20-22hr    42 embryo 22-24hr    57 larva L1    54 larva L2    74 larva L3 12hr old    58 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    60 white prepupae new    62 white prepupae 12hr    46 white prepupae 24hr    44 pupae 2d postWPP    45 pupae 3d postWPP    54 pupae 4d postWPP    19 adult male 01day    34 adult male 05day    23 adult male 30day    16 adult female 01day    35 adult female 05day    71 adult female 30day    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (50) embryo 02-04hr  26 embryo 04-06hr  (53) embryo 06-08hr  (56) embryo 08-10hr  (65) embryo 10-12hr  (48) embryo 12-14hr  (93) embryo 14-16hr  (39) embryo 16-18hr  (54) embryo 18-20hr  (34) embryo 20-22hr  (42) embryo 22-24hr  (57) larva L1  (54) larva L2  (74) larva L3 12hr old  (58) larva L3 puffstage 1-2  (28) larva L3 puffstage 3-6  (42) larva L3 puffstage 7-9  (60) white prepupae new  (62) white prepupae 12hr  (46) white prepupae 24hr  (44) pupae 2d postWPP  (45) pupae 3d postWPP  (54) pupae 4d postWPP    19 adult male 01day  (34) adult male 05day    23 adult male 30day    16 adult female 01day  (35) adult female 05day  (71) adult female 30day  (55) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    26 embryo 04-06hr    53 embryo 06-08hr    56 embryo 08-10hr    65 embryo 10-12hr    48 embryo 12-14hr    93 embryo 14-16hr    39 embryo 16-18hr    54 embryo 18-20hr    34 embryo 20-22hr    42 embryo 22-24hr    57 larva L1    54 larva L2    74 larva L3 12hr old    58 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    60 white prepupae new    62 white prepupae 12hr    46 white prepupae 24hr    44 pupae 2d postWPP    45 pupae 3d postWPP    54 pupae 4d postWPP    19 adult male 01day    34 adult male 05day    23 adult male 30day    16 adult female 01day    35 adult female 05day    71 adult female 30day    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    26 embryo 04-06hr    53 embryo 06-08hr    56 embryo 08-10hr    65 embryo 10-12hr    48 embryo 12-14hr    93 embryo 14-16hr    39 embryo 16-18hr    54 embryo 18-20hr    34 embryo 20-22hr    42 embryo 22-24hr    57 larva L1    54 larva L2    74 larva L3 12hr old    58 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    60 white prepupae new    62 white prepupae 12hr    46 white prepupae 24hr    44 pupae 2d postWPP    45 pupae 3d postWPP    54 pupae 4d postWPP    19 adult male 01day    34 adult male 05day    23 adult male 30day    16 adult female 01day    35 adult female 05day    71 adult female 30day    55 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    26 embryo 04-06hr    53 embryo 06-08hr    56 embryo 08-10hr    65 embryo 10-12hr    48 embryo 12-14hr    93 embryo 14-16hr    39 embryo 16-18hr    54 embryo 18-20hr    34 embryo 20-22hr    42 embryo 22-24hr    57 larva L1    54 larva L2    74 larva L3 12hr old    58 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    60 white prepupae new    62 white prepupae 12hr    46 white prepupae 24hr    44 pupae 2d postWPP    45 pupae 3d postWPP    54 pupae 4d postWPP    19 adult male 01day    34 adult male 05day    23 adult male 30day    16 adult female 01day    35 adult female 05day    71 adult female 30day    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (50) embryo 02-04hr  26 embryo 04-06hr  (53) embryo 06-08hr  (56) embryo 08-10hr  (65) embryo 10-12hr  (48) embryo 12-14hr  (93) embryo 14-16hr  (39) embryo 16-18hr  (54) embryo 18-20hr  (34) embryo 20-22hr  (42) embryo 22-24hr  (57) larva L1  (54) larva L2  (74) larva L3 12hr old  (58) larva L3 puffstage 1-2  28 larva L3 puffstage 3-6  (42) larva L3 puffstage 7-9  (60) white prepupae new  (62) white prepupae 12hr  (46) white prepupae 24hr  (44) pupae 2d postWPP  (45) pupae 3d postWPP  (54) pupae 4d postWPP    19 adult male 01day  (34) adult male 05day    23 adult male 30day    16 adult female 01day  (35) adult female 05day  (71) adult female 30day  (55) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    26 embryo 04-06hr    53 embryo 06-08hr    56 embryo 08-10hr    65 embryo 10-12hr    48 embryo 12-14hr    93 embryo 14-16hr    39 embryo 16-18hr    54 embryo 18-20hr    34 embryo 20-22hr    42 embryo 22-24hr    57 larva L1    54 larva L2    74 larva L3 12hr old    58 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    60 white prepupae new    62 white prepupae 12hr    46 white prepupae 24hr    44 pupae 2d postWPP    45 pupae 3d postWPP    54 pupae 4d postWPP    19 adult male 01day    34 adult male 05day    23 adult male 30day    16 adult female 01day    35 adult female 05day    71 adult female 30day    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    50 embryo 02-04hr    26 embryo 04-06hr    53 embryo 06-08hr    56 embryo 08-10hr    65 embryo 10-12hr    48 embryo 12-14hr    93 embryo 14-16hr    39 embryo 16-18hr    54 embryo 18-20hr    34 embryo 20-22hr    42 embryo 22-24hr    57 larva L1    54 larva L2    74 larva L3 12hr old    58 larva L3 puffstage 1-2    28 larva L3 puffstage 3-6    42 larva L3 puffstage 7-9    60 white prepupae new    62 white prepupae 12hr    46 white prepupae 24hr    44 pupae 2d postWPP    45 pupae 3d postWPP    54 pupae 4d postWPP    19 adult male 01day    34 adult male 05day    23 adult male 30day    16 adult female 01day    35 adult female 05day    71 adult female 30day    55 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    69 Schneider line 2 Sg4    42 embryonic 1182-4H    82 embryonic GM2    98 embryonic Kc167    58 embryonic S1    65 embryonic S3    169 leg disc CME L1    44 wing disc CME-W2    152 wing disc ML-DmD8    52 wing disc ML-DmD9    44 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    13 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    164 ovary fGS/OSS    49 ovary OSC    113 ovary OSS    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (69) Schneider line 2 Sg4  (42) embryonic 1182-4H  (82) embryonic GM2  (98) embryonic Kc167  (58) embryonic S1  (65) embryonic S3  (169) leg disc CME L1  (44) wing disc CME-W2  (152) wing disc ML-DmD8  (52) wing disc ML-DmD9  (44) wing disc ML-DmD16-c3  (101) wing disc ML-DmD21    13 wing disc ML-DmD32  (47) haltere disc ML-DmD17-c3  (102) eye-antennal disc ML-DmD11  (74) antennal disc ML-DmD20-c5  (40) mixed discs ML-DmD4-c1  (73) CNS ML-DmBG1-c1  (62) CNS ML-DmBG2-c2  (71) tumorous blood cells mbn2  (164) ovary fGS/OSS  (49) ovary OSC  (113) ovary OSS  (49) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    69 Schneider line 2 Sg4    42 embryonic 1182-4H    82 embryonic GM2    98 embryonic Kc167    58 embryonic S1    65 embryonic S3  (169) leg disc CME L1    44 wing disc CME-W2  (152) wing disc ML-DmD8    52 wing disc ML-DmD9    44 wing disc ML-DmD16-c3  101 wing disc ML-DmD21    13 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3  102 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2  (164) ovary fGS/OSS    49 ovary OSC  (113) ovary OSS    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    69 Schneider line 2 Sg4    42 embryonic 1182-4H    82 embryonic GM2    98 embryonic Kc167    58 embryonic S1    65 embryonic S3    169 leg disc CME L1    44 wing disc CME-W2    152 wing disc ML-DmD8    52 wing disc ML-DmD9    44 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    13 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    164 ovary fGS/OSS    49 ovary OSC    113 ovary OSS    49 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    69 Schneider line 2 Sg4    42 embryonic 1182-4H    82 embryonic GM2    98 embryonic Kc167    58 embryonic S1    65 embryonic S3    169 leg disc CME L1    44 wing disc CME-W2    152 wing disc ML-DmD8    52 wing disc ML-DmD9    44 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    13 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    164 ovary fGS/OSS    49 ovary OSC    113 ovary OSS    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (69) Schneider line 2 Sg4  (42) embryonic 1182-4H  (82) embryonic GM2  (98) embryonic Kc167  (58) embryonic S1  (65) embryonic S3  (169) leg disc CME L1  (44) wing disc CME-W2  (152) wing disc ML-DmD8  (52) wing disc ML-DmD9  (44) wing disc ML-DmD16-c3  (101) wing disc ML-DmD21    13 wing disc ML-DmD32  (47) haltere disc ML-DmD17-c3  (102) eye-antennal disc ML-DmD11  (74) antennal disc ML-DmD20-c5  (40) mixed discs ML-DmD4-c1  (73) CNS ML-DmBG1-c1  (62) CNS ML-DmBG2-c2  (71) tumorous blood cells mbn2  (164) ovary fGS/OSS  (49) ovary OSC  (113) ovary OSS  (49) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    69 Schneider line 2 Sg4    42 embryonic 1182-4H    82 embryonic GM2    98 embryonic Kc167    58 embryonic S1    65 embryonic S3  (169) leg disc CME L1    44 wing disc CME-W2  (152) wing disc ML-DmD8    52 wing disc ML-DmD9    44 wing disc ML-DmD16-c3  101 wing disc ML-DmD21    13 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3  102 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2  (164) ovary fGS/OSS    49 ovary OSC  113 ovary OSS    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    69 Schneider line 2 Sg4    42 embryonic 1182-4H    82 embryonic GM2    98 embryonic Kc167    58 embryonic S1    65 embryonic S3    169 leg disc CME L1    44 wing disc CME-W2    152 wing disc ML-DmD8    52 wing disc ML-DmD9    44 wing disc ML-DmD16-c3    101 wing disc ML-DmD21    13 wing disc ML-DmD32    47 haltere disc ML-DmD17-c3    102 eye-antennal disc ML-DmD11    74 antennal disc ML-DmD20-c5    40 mixed discs ML-DmD4-c1    73 CNS ML-DmBG1-c1    62 CNS ML-DmBG2-c2    71 tumorous blood cells mbn2    164 ovary fGS/OSS    49 ovary OSC    113 ovary OSS    49 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult    68 Cadmium 50 mM 6 hrs, larvae L3    40 Cadmium 50 mM 12 hrs, larvae L3    69 Cadmium 50 mM 48 hrs, 4-day adult    74 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    106 Zinc 5 mM 12 hrs, larvae L3    31 Zinc 4.5 mM 48 hrs, 4-day adult    57 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    67 Ethanol 10% 3 hrs, larvae L3    47 Caffeine 1.5 mg/ml 4 hrs, larvae L3    56 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    37 Paraquat 5 mM 48 hrs, 4-day adult    74 Paraquat 10 mM 48 hrs, 4-day adult    57 Rotenone 2 μg 12 hrs, larvae L3    67 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult  (68) Cadmium 50 mM 6 hrs, larvae L3  (40) Cadmium 50 mM 12 hrs, larvae L3  (69) Cadmium 50 mM 48 hrs, 4-day adult  (74) Cadmium 100 mM 48 hrs, 4-day adult  (87) Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  (106) Zinc 5 mM 12 hrs, larvae L3  (31) Zinc 4.5 mM 48 hrs, 4-day adult  (57) Ethanol 2.5% 3 hrs, larvae L3  (61) Ethanol 5% 3 hrs, larvae L3  (67) Ethanol 10% 3 hrs, larvae L3  (47) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (56) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (82) Caffeine 25 mg/ml 48 hrs, 4-day adult  (37) Paraquat 5 mM 48 hrs, 4-day adult  (74) Paraquat 10 mM 48 hrs, 4-day adult  (57) Rotenone 2 μg 12 hrs, larvae L3  (67) Rotenone 8 μg 12 hrs, larvae L3  (28) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult    68 Cadmium 50 mM 6 hrs, larvae L3    40 Cadmium 50 mM 12 hrs, larvae L3    69 Cadmium 50 mM 48 hrs, 4-day adult    74 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  106 Zinc 5 mM 12 hrs, larvae L3    31 Zinc 4.5 mM 48 hrs, 4-day adult    57 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    67 Ethanol 10% 3 hrs, larvae L3    47 Caffeine 1.5 mg/ml 4 hrs, larvae L3    56 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    37 Paraquat 5 mM 48 hrs, 4-day adult    74 Paraquat 10 mM 48 hrs, 4-day adult    57 Rotenone 2 μg 12 hrs, larvae L3    67 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult    68 Cadmium 50 mM 6 hrs, larvae L3    40 Cadmium 50 mM 12 hrs, larvae L3    69 Cadmium 50 mM 48 hrs, 4-day adult    74 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    106 Zinc 5 mM 12 hrs, larvae L3    31 Zinc 4.5 mM 48 hrs, 4-day adult    57 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    67 Ethanol 10% 3 hrs, larvae L3    47 Caffeine 1.5 mg/ml 4 hrs, larvae L3    56 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    37 Paraquat 5 mM 48 hrs, 4-day adult    74 Paraquat 10 mM 48 hrs, 4-day adult    57 Rotenone 2 μg 12 hrs, larvae L3    67 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult    68 Cadmium 50 mM 6 hrs, larvae L3    40 Cadmium 50 mM 12 hrs, larvae L3    69 Cadmium 50 mM 48 hrs, 4-day adult    74 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    106 Zinc 5 mM 12 hrs, larvae L3    31 Zinc 4.5 mM 48 hrs, 4-day adult    57 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    67 Ethanol 10% 3 hrs, larvae L3    47 Caffeine 1.5 mg/ml 4 hrs, larvae L3    56 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    37 Paraquat 5 mM 48 hrs, 4-day adult    74 Paraquat 10 mM 48 hrs, 4-day adult    57 Rotenone 2 μg 12 hrs, larvae L3    67 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult  (68) Cadmium 50 mM 6 hrs, larvae L3  (40) Cadmium 50 mM 12 hrs, larvae L3  (69) Cadmium 50 mM 48 hrs, 4-day adult  (74) Cadmium 100 mM 48 hrs, 4-day adult  (87) Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  (106) Zinc 5 mM 12 hrs, larvae L3  31 Zinc 4.5 mM 48 hrs, 4-day adult  (57) Ethanol 2.5% 3 hrs, larvae L3  (61) Ethanol 5% 3 hrs, larvae L3  (67) Ethanol 10% 3 hrs, larvae L3  (47) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (56) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (82) Caffeine 25 mg/ml 48 hrs, 4-day adult  (37) Paraquat 5 mM 48 hrs, 4-day adult  (74) Paraquat 10 mM 48 hrs, 4-day adult  (57) Rotenone 2 μg 12 hrs, larvae L3  (67) Rotenone 8 μg 12 hrs, larvae L3  28 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult    68 Cadmium 50 mM 6 hrs, larvae L3    40 Cadmium 50 mM 12 hrs, larvae L3    69 Cadmium 50 mM 48 hrs, 4-day adult    74 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult  106 Zinc 5 mM 12 hrs, larvae L3    31 Zinc 4.5 mM 48 hrs, 4-day adult    57 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    67 Ethanol 10% 3 hrs, larvae L3    47 Caffeine 1.5 mg/ml 4 hrs, larvae L3    56 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    37 Paraquat 5 mM 48 hrs, 4-day adult    74 Paraquat 10 mM 48 hrs, 4-day adult    57 Rotenone 2 μg 12 hrs, larvae L3    67 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    25 cold shock, 4-day adult    23 heat shock, 4-day adult    68 Cadmium 50 mM 6 hrs, larvae L3    40 Cadmium 50 mM 12 hrs, larvae L3    69 Cadmium 50 mM 48 hrs, 4-day adult    74 Cadmium 100 mM 48 hrs, 4-day adult    87 Copper 0.5 mM 12 hrs, larvae L3    19 Copper 15 mM 48 hrs, 4-day adult    106 Zinc 5 mM 12 hrs, larvae L3    31 Zinc 4.5 mM 48 hrs, 4-day adult    57 Ethanol 2.5% 3 hrs, larvae L3    61 Ethanol 5% 3 hrs, larvae L3    67 Ethanol 10% 3 hrs, larvae L3    47 Caffeine 1.5 mg/ml 4 hrs, larvae L3    56 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    82 Caffeine 25 mg/ml 48 hrs, 4-day adult    37 Paraquat 5 mM 48 hrs, 4-day adult    74 Paraquat 10 mM 48 hrs, 4-day adult    57 Rotenone 2 μg 12 hrs, larvae L3    67 Rotenone 8 μg 12 hrs, larvae L3    28 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_03 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	BDGP expression data • PC00134 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023213 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10811-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal, with Scer\GAL4pnr-MD237 eIF4GGD7098 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of eIF4G Class Mutagen Stocks Known lesion eIF4Ge02087 piggyBac transposase 1 -- eIF4GCPTI002576 piggyBac transposase 0 Yes
Alleles Carried on Transgenic Constructs ( 3 )
For All Alleles Carried on Transgenic Constructs Show
Allele of eIF4G Class Mutagen Stocks Known lesion eIF4GGD7098 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes eIF4GHMS00762 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes eIF4GdsRNA.cHa in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD7098} NA P{TRiP.HMS00762} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{754.P.FSVS-0}eIF4GCPTI002576 NA PBac{RB}eIF4Ge02087 NA
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 10 terms )
Molecular Function
CV term Evidence References
eukaryotic initiation factor 4E binding inferred from physical interaction with eIF4E-3 (Hernandez et al., 2005) inferred from physical interaction with eIF4E-4 (Hernandez et al., 2005) inferred from physical interaction with eIF4E-5 (Hernandez et al., 2005) inferred from physical interaction with eIF4E-7 (Hernandez et al., 2005) inferred from physical interaction with eIF-4E (Hernandez et al., 2005, Hernandez et al., 1998) RNA 7-methylguanosine cap binding inferred from direct assay (Franklin-Dumont et al., 2007) RNA binding inferred from direct assay (Zapata et al., 1994)
Biological Process
CV term Evidence References
G1/S transition of mitotic cell cycle inferred from mutant phenotype (Franklin-Dumont et al., 2007) mitosis inferred from mutant phenotype (Ducat et al., 2008) mitotic G2 DNA damage checkpoint inferred from genetic interaction with nbs (Kondo and Perrimon, 2011) mitotic spindle elongation inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) translation inferred from direct assay (Zapata et al., 1994)
Cellular Component
CV term Evidence References
eukaryotic translation initiation factor 4F complex inferred from direct assay (Zapata et al., 1994)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from electronic annotation with InterPro:IPR003890 (FlyBase Curators et al., 2004-) translation initiation factor activity inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with human EIF4G1, Saccharomyces TIF4631 (Hernandez et al., 1998)
Biological Process
CV term Evidence References
RNA metabolic process inferred from electronic annotation with InterPro:IPR003890, InterPro:IPR016021 (FlyBase Curators et al., 2004-) translational initiation inferred from sequence or structural similarity (Lasko, 2000) inferred from sequence or structural similarity with human EIF4G1, Saccharomyces TIF4631 (Hernandez et al., 1998)
Cellular Component
CV term Evidence References
cytosol inferred from sequence or structural similarity (Lasko, 2000) eukaryotic translation initiation factor 4F complex inferred from sequence or structural similarity (Lasko, 2000)
Sequence Ontology: Class of Gene
	gene_with_edited_transcript (Graveley et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
eIF4G - Capr anti bait coimmunoprecipitation, western blot (Papoulas et al., 2010) eIF4G - Fmr1 anti bait coimmunoprecipitation, peptide massfingerprinting, western blot (Papoulas et al., 2010) eIF4G - eIF-4E anti bait coimmunoprecipitation, western blot (Hernandez et al., 2012) eIF4G - eIF4E-3 anti bait coimmunoprecipitation, western blot (Hernandez et al., 2012)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions eIF4G allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68664 DroID - A comprehensive database of gene and protein interactions. • FBgn0023213 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43839
Orthologs
OrthoDB Orthologs (96) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG683DPV Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG60K6TQ Arthropod inclusive ortholog search EOG6FN2ZN Metazoa inclusive ortholog search EOG6WQCV7
Orthologs in Drosophila Species (EOG683DPV)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\eIF4G     Drosophila sechellia   Dsec\GM26760 Y   Drosophila erecta   Dere\GG16429 Y   Drosophila yakuba   Dyak\GE14497 Y   Drosophila ananassae   Dana\GF19009 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10575 Y   Drosophila persimilis   Dper\GL15607 Y   Drosophila willistoni   Dwil\GK13658 Y   Drosophila virilis   Dvir\GJ21224 Y   Drosophila mojavensis   Dmoj\GI23530     Drosophila mojavensis   Dmoj\GI14106 Y   Drosophila grimshawi   Dgri\GH23978 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG60K6TQ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB45348 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG037316 Y Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG018209 Y Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15947 Y Atta cephalotes Leafcutter ant  Acep\ACEP14634 Y Atta cephalotes Leafcutter ant  Acep\ACEP14625 Y Camponotus floridanus Florida carpenter ant  Cflo\CFLO19603 Y Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL18360 Y Linepithema humile Argentine ant  Lhum\LH12239 Y Pogonomyrmex barbatus Red harvester ant  Pbar\PB20204 Y Solenopsis invicta Red fire ant  Sinv\SINV17648 Y Solenopsis invicta Red fire ant  Sinv\SINV18171 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI000625 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI48547 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI41323 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI069178 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI070526 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI073861 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI31627 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI004413 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI39421 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI070472 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI52739 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI060566 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI062529 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI067845 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI082641 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI41153 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI005288 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI086687 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI067875 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI32865 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI30966 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI39422 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI061549 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI006471 Y Acyrthosiphon pisum Pea aphid  Apim\ACYPI002206 Y Bombyx mori Silkmoth  Bmor\BGIBMGA000528 Y Pediculus humanus Human body louse  Phum\PHUM067390 Y Tribolium castaneum Red flour beetle  Tcas\TC013876 Y
Orthologs in non-Insect Arthropods (EOG6FN2ZN)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\SRPX2 Y Daphnia pulex Water flea  Dpux\JGI_V11_244701 Y Daphnia pulex Water flea  Dpux\JGI_V11_5900 Y Daphnia pulex Water flea  Dpux\JGI_V11_225020 Y Daphnia pulex Water flea  Dpux\CDC55 Y Daphnia pulex Water flea  Dpux\JGI_V11_248524 Y Daphnia pulex Water flea  Dpux\EDNRA Y Daphnia pulex Water flea  Dpux\Pnpla8 Y Daphnia pulex Water flea  Dpux\JGI_V11_5957 Y Daphnia pulex Water flea  Dpux\CoI Y Daphnia pulex Water flea  Dpux\JGI_V11_228894 Y Daphnia pulex Water flea  Dpux\JGI_V11_55191 Y Daphnia pulex Water flea  Dpux\JGI_V11_198899 Y Ixodes scapularis Deer tick  Isca\ISCW008595 Y Ixodes scapularis Deer tick  Isca\ISCW008594 Y
Orthologs in non-Arthropod Metazoa (EOG6WQCV7)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\ifg-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_023932gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_019064gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028861gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_006085gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024859gn Y Danio rerio Zebrafish  Drer\wu:fb16f03 Y Danio rerio Zebrafish  Drer\CABZ01085054.1 Y Danio rerio Zebrafish  Drer\ENSDARG00000077215 Y Danio rerio Zebrafish  Drer\eif4g2 Y Danio rerio Zebrafish  Drer\eif4g2a Y Danio rerio Zebrafish  Drer\OTTDARG00000030376 Y Danio rerio Zebrafish  Drer\ENSDARG00000006200 Y Xenopus tropicalis Western clawed frog  Xtro\eif4g2 Y Xenopus tropicalis Western clawed frog  Xtro\eif4g3 Y Xenopus tropicalis Western clawed frog  Xtro\eif4g1 Y Gallus gallus Domestic chicken  Ggal\EIF4G1 Y Gallus gallus Domestic chicken  Ggal\EIF4G3 Y Gallus gallus Domestic chicken  Ggal\EIF4G2 Y Mus musculus House mouse  Mmus\Eif4g2 Y Mus musculus House mouse  Mmus\Eif4g1 Y Mus musculus House mouse  Mmus\Eif4g3 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000017158 Y Rattus norvegicus Norway rat  Rnor\Eif4g1 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000030704 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000014368 Y Homo sapiens Human  Hsap\EIF4G1 Y Homo sapiens Human  Hsap\EIF4G2 Y Homo sapiens Human  Hsap\EIF4G3 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\EIF4G1     Hsap\EIF4G2     Hsap\EIF4G3
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM26760 Drosophila erecta Dere\GG16429 Drosophila yakuba Dyak\GE14497 Drosophila ananassae Dana\GF19009 Drosophila pseudoobscura pseudoobscura Dpse\GA10575 Drosophila persimilis Dper\GL15607 Drosophila willistoni Dwil\GK13658 Drosophila virilis Dvir\GJ21224 Drosophila mojavensis Dmoj\GI14106 Drosophila grimshawi Dgri\GH23978
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	33049 y1 sc* v1; P{TRiP.HMS00762}attP2
Harvard	- PBac{RB}eIF4Ge02087
VDRC	v17002 w1118; P{GD7098}v17002 v17003 w1118; P{GD7098}v17003
Genomic Clones ( 3 )
	BACR11A04 BACR13D24 BACR16N23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 124 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE51676
Other clones	FI02056
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LP09265 LP20066 RE27713 RE31602 SD15021
Other clones	17243 28124 31287 38343 43767 56629 57571 62481 73458 82090 83002 102462 117383 120766 121403 145162 146875 153564 174007 180688 183172 191798 214275.13 214445.8 CK01.32D.C1 CK01.59A.F6 CK01.72C.F10 CK01.73C.A2 CK01.94C.B8 CK01.98C.F6 CK01.104B.C4 CK01.104B.G4 CK01.107B.G2 CK01.112B.G4 CK01.123C.C3 CK01.126A.B6 Dmel_dig_019713_0672_3407 Dmel_neb_009634_0360_1406 Dmel_neb_011291_0570_3087 Dmel_neb_011382_0620_1304 Dmel_neb_012613_0640_2196 Dmel_neb_013409_0937_3431 Dmel_neb_013744_0895_0706 Dmel_neb_014734_0202_1385 EC24329 EC27028 EC34728 EC258826 EK020732 EK034831 EK039456 EK047842 EK057553 EK057933 EK065641 EK065939 EK073640 EK076142 EK081557 EK087458 EK089557 EK093603 EK094010 EK110232 EK129337 EK135543 EK140464 EK151529 EK157708 EK160361 EK168933 EK184836 EK188043 EK206504 EK216626 EK217317 EK225552 EK236247 EK240942 EK241311 EK245128 EK250560 EK252609 EK252817 EK257038 EK288036 EK294717 EP02124 EP07902 EP09034 EP22141 ESG0117c.B21_B12 FGC012A10 FGH123A11 FGM104A08 FGM219A03 FGM225H12 GM10051 MB6-CG10811-RB RP004114232 RP004123202 RP004170705 RP004176285 RP004179813 RP004215176 RP004220313 RP004225129 RP004235675 RP004240262 RP004270083 RP004323620 RP004416536 RP004462859 RP004497384 RP004521811 RP004531233 RP004536543
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0023213 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43839
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: eIF4G eIF-4G (Fuller and Baker, 2007.6.28)
Source for database merge of
Additional comments
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in aberrantly short, monopolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) eIF-4G has been cloned and sequenced. (Hernandez et al., 1998) Drosophila initiation factor eIF-4F is composed of two subunits, one is encoded by eIF-4E and the other by eIF-4G, a 200kD protein. eIF-4G is required for the translation of endogenous mRNAs in cell-free systems made from Drosophila embryos, and can cross-link uncapped, but not m7G capped RNA. eIF-4E cross-links with m7G capped RNA. (Zapata et al., 1994)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079901 NM_001103382 NM_001144385 RefSeq (Proteins) • NP_524640 NP_001096852 NP_001137857 DDBJ / EMBL / GenBank • AA735894 AAC38985 AAF59403 AAQ22456 ABV53593 AC010916 ACL82868 AF030155 AW941972 BI366194 BT009987 CZ479912 Entrez Gene - A searchable database of RefSeq genes. • 43839 UniProtKB/TrEMBL • A8DZ29 O61380 Q7YU50
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • W2 domain (IPR003307) MIF4G-like, type 3 (IPR003890) Initiation factor eIF-4 gamma, MA3 (IPR003891) MIF4-like, type 1/2/3 (IPR016021) Armadillo-type fold (IPR016024) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • PC00134
Linkouts
	BioGRID - A database of protein and genetic interactions • 68664 DroID - A comprehensive database of gene and protein interactions. • FBgn0023213 DRSC - Results from RNAi screens. • FBgn0023213 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023213 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10811-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0023213 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 43839 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 43839 modMine - Data generated by the modENCODE project. • FBgn0023213
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	CG10811 (Labourier et al., 2002, Lasko, 2000, Franklin-Dumont et al., 2007, Fuller and Baker, 2007.6.28, Chen et al., 2012, Goshima et al., 2007, Franklin-Dumont et al., 2007, Baker and Fuller, 2007, Ducat et al., 2008, Pisa et al., 2009, Jäger and Dorner, 2010, Ni et al., 2010.12.1) deIF4G (Takahashi et al., 2005, Garrey et al., ) eIF4G (Macdonald, 2005, Zappavigna et al., 2004, Kozak, 2004, Macdonald, 2004, Herold et al., 2003, Gamberi et al., 2003, Herold et al., 2001, Johnstone and Lasko, 2001, Lasko, 2000, Locke et al., 2000, Lehner, 1999, Hernandez et al., 1998, Baker and Fuller, 2006, Franklin-Dumont et al., 2007, Hernandez et al., 2005, Fuller and Baker, 2007.6.28, Hernandez et al., 2008, Franklin-Dumont et al., 2007, Zekri et al., 2009, Baker and Fuller, 2007, Iwasaki et al., 2009, Hernandez et al., 2012, Pisa et al., 2009, Mihailovich et al., 2012, Duncan et al., 2009, Jäger and Dorner, 2010, Papoulas et al., 2010, Garrey et al., , Wasbrough et al., 2010, Hernandez and Vazquez-Pianzola, 2005, Hernandez et al., 2004, Duncan, 2008, Killip and Grewal, 2012) eIF4g (Ephrussi and St. Johnston, 2004) Eif4G   eIF-4G (Fuller and Baker, 2007.6.28, Greil et al., 2003, Bjorklund et al., 2006, Stuart et al., 2007, Valencia-Sanchez et al., 2006, Goshima et al., 2007, Johansson et al., 2007, Tadros et al., 2007, Ducat et al., 2008) EIF4G (Kondo and Perrimon, 2011) p200 (Zapata et al., 1994)
Name Synonym	eIF-4G (Johansson et al., 2007) Eukaryotic-initiation-factor-4G   Eukaryotic initiation factor 4G (Gouw et al., 2009) eukaryotic initiation factor 4G (Papoulas et al., 2010) eukaryotic translation initiation factor 4G (Pisa et al., 2009, Garrey et al., ) translation initiation factor (Cho et al., 2005) translation initiation factor eIF4G (Hernandez, 1997.10.16)
Secondary FlyBase IDs
References ( 72 )
Generate a list of	All references relating to this gene ( 72 )
List References by type	Research paper  ( 37 ) Supplementary material  ( 7 ) Review  ( 8 ) Personal communication to FlyBase  ( 9 ) Abstract  ( 6 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 7 )
	Chen et al., 2012, Cell Death Differ. 19(10): 1655--1663 Effectors of alcohol-induced cell killing in Drosophila. [FBrf0219410] Fukaya and Tomari, 2012, Mol. Cell 48(6): 825--836 MicroRNAs Mediate Gene Silencing via Multiple Different Pathways in Drosophila. [FBrf0220346] Hernandez et al., 2012, Development 139(17): 3211--3220 Eukaryotic initiation factor 4E-3 is essential for meiotic chromosome segregation, cytokinesis and male fertility in Drosophila. [FBrf0219080] Killip and Grewal, 2012, Dev. Biol. 371(2): 191--202 DREF is required for cell and organismal growth in Drosophila and functions downstream of the nutrition/TOR pathway. [FBrf0219592] Mihailovich et al., 2012, RNA 18(1): 53--64 Widespread generation of alternative UTRs contributes to sex-specific RNA binding by UNR. [FBrf0216923] Jeske et al., 2011, EMBO J. 30(1): 90--103 Smaug assembles an ATP-dependent stable complex repressing nanos mRNA translation at multiple levels. [FBrf0212721] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...