FlyBase Gene Report: Dmel\Dap160

General Information

Symbol

Dmel\Dap160

Species

D. melanogaster

Name

Dynamin associated protein 160

Annotation Symbol

CG1099

Feature Type

protein_coding_gene

FlyBase ID

FBgn0023388

Gene Model Status

Current

Stock Availability

12 publicly available

Gene Summary

Dynamin associated protein 160 (Dap160) encodes an adaptor protein that contributes to endocytosis. It regulates the Notch pathway and mediates the asymmetric accumulation of a number or proteins, including the product of aPKC during neuroblast division. [Date last reviewed: 2019-09-12] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Pathway
Interactive Fly

Also Known As

intersectin, Dap160/intersectin, noncoding_4110, DAP-160

Key Links

Genomic Location

Cytogenetic map

39A3-39A4

Sequence location

2L:21,134,439..21,142,347 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:21134439..21142347

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (18 terms)

Molecular Function (5 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

enables protein binding

inferred from physical interaction with FLYBASE:par-6; FB:FBgn0026192

(Chabu and Doe, 2008)

inferred from physical interaction with FLYBASE:aPKC; FB:FBgn0261854

(Chabu and Doe, 2008)

enables protein kinase activator activity

inferred from direct assay

(Chabu and Doe, 2008)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

enables calcium ion binding

inferred from electronic annotation with InterPro:IPR002048

(InterPro Project Members, 2004-)

enables nucleic acid binding

inferred from electronic annotation with InterPro:IPR035979

(InterPro Project Members, 2004-)

enables protein-macromolecule adaptor activity

inferred from biological aspect of ancestor with PANTHER:PTN000130974

(GO Reference Genome Project, 2011-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

involved_in bulk synaptic vesicle endocytosis

inferred from mutant phenotype

(Koh et al., 2004)

involved_in clathrin-dependent synaptic vesicle endocytosis

inferred from mutant phenotype

(Koh et al., 2004)

involved_in intracellular protein localization

inferred from mutant phenotype

(Chabu and Doe, 2008, Koh et al., 2004)

involved_in negative regulation of Notch signaling pathway

inferred from mutant phenotype

(Tang et al., 2005)

involved_in positive regulation of neuroblast proliferation

inferred from mutant phenotype

(Chabu and Doe, 2008)

involved_in synaptic vesicle endocytosis

inferred from mutant phenotype

(Koh et al., 2007, Marie et al., 2004)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in clathrin-dependent synaptic vesicle endocytosis

inferred from biological aspect of ancestor with PANTHER:PTN000880482

(GO Reference Genome Project, 2011-)

involved_in endosomal transport

inferred from biological aspect of ancestor with PANTHER:PTN000130974

(GO Reference Genome Project, 2011-)

Cellular Component (6 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

located_in apical cortex

inferred from direct assay

(Chabu and Doe, 2008)

located_in cytoplasm

inferred from direct assay

(Chabu and Doe, 2008)

colocalizes_with presynaptic membrane

inferred from direct assay

(Marie et al., 2004)

located_in synaptic vesicle

inferred from direct assay

(Jiao et al., 2010)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000130974

(GO Reference Genome Project, 2011-)

is_active_in intracellular vesicle

inferred from biological aspect of ancestor with PANTHER:PTN000880482

(GO Reference Genome Project, 2011-)

is_active_in plasma membrane

inferred from biological aspect of ancestor with PANTHER:PTN000130974

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

Pathway (FlyBase)

NEGATIVE REGULATORS OF NOTCH SIGNALING PATHWAY

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Pathway (FlyBase)

NEGATIVE REGULATORS OF NOTCH SIGNALING PATHWAY -: The Notch receptor signaling pathway is activated by the binding of the transmembrane receptor Notch (N) to transmembrane ligands, Dl or Ser, presented on adjacent cells. This results in the proteolytic cleavage of N, releasing the intracellular domain (NICD). NICD translocates into the nucleus, interacting with Su(H) and mam to form a transcription complex, which up-regulates transcription of Notch-responsive genes. Negative regulators of the pathway down-regulate the signal from the sending cell or the response in the receiving cell. (Adapted from FBrf0225731 and FBrf0192604).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\Dap160 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry M9ND00)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.

Gene model reviewed during 5.46

Tissue-specific extension of 3' UTRs observed during later stages (FBrf0218523, FBrf0219848); all variants may not be annotated

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

Dap160-RA

FBtr0081503

NM_057974

3973

1097

Dap160-RB

FBtr0081504

NM_057975

3724

1014

Dap160-RC

FBtr0301276

NM_001169557

3970

1096

Dap160-RD

FBtr0306017

NM_001259189

4168

1190

Dap160-RG

FBtr0333086

NM_001273734

5420

1088

Dap160-RH

FBtr0333087

NM_001273735

5171

1005

Additional Transcript Data and Comments

Reported size (kB)

4.0, 3.8 (longest cDNA)

(Roos and Kelly, 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

Dap160-PA

FBpp0081031

120.8

1097

4.90

FBpp0081032

111.9

1014

5.16

FBpp0290491

120.6

1096

4.92

FBpp0297159

131.7

1190

5.17

FBpp0305299

119.9

1088

4.80

FBpp0305300

111.0

1005

5.00

M9PDC7

NP_001260664

AGB93199

Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments

Reported size (kDa)

1097, 1014 (aa); 160 (kD observed)

(Roos and Kelly, 1998)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Dap160 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dap160_1

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.92

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 11 -- 12

lateral cord neuron

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic foregut sensory structure

(Fisher et al., 2012)

embryonic head epidermis

(Fisher et al., 2012)

embryonic head sensory system

(Fisher et al., 2012)

larval nervous system

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

larval ventral nerve cord

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immuno-electron microscopy

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

neuromuscular junction

(Winther et al., 2013)

immunohistochemistry

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

motor neuron

• presynaptic active zone

Comment: periactive zone

(Marie et al., 2004)

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

larval stage

neuromuscular junction | restricted

Comment: Presynaptic

(Verstreken et al., 2009)

third instar larval stage

neuromuscular junction

(Winther et al., 2013, Roos and Kelly, 1998)

bouton

(Roos and Kelly, 1998)

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult head

• membrane

(Aradska et al., 2015)

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

head

(Kelly and Phillips, 2005)

Additional Descriptive Data

Dap160 protein is restricted to synaptic terminals in third instar neuromuscular junctions.

(Roos and Kelly, 1998)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in apical cortex

inferred from direct assay

(Chabu and Doe, 2008)

located_in cytoplasm

inferred from direct assay

(Chabu and Doe, 2008)

colocalizes_with presynaptic membrane

inferred from direct assay

(Marie et al., 2004)

located_in synaptic vesicle

inferred from direct assay

(Jiao et al., 2010)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Dap160 in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\Dap160

View exonic expression by tissue for Dmel\Dap160

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023388

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35378/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0023388

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0023388

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0023388

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1099

Flygut - An atlas of the Drosophila adult midgut

FBgn0023388

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\Dap160

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 21 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 16 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of Dap160

Transgenic constructs containing regulatory region of Dap160

Aberrations (Deficiencies and Duplications) ( 9 )

Inferred from experimentation ( 9 )

Gene disrupted in

Df(2L)10523-35

(Marie et al., 2004)

Df(2L)bur-K1

(Koh et al., 2004)

Df(2L)Dap160^2543-Δ1

(Marie et al., 2004)

Df(2L)DS8

(Koh et al., 2004)

Df(2L)Exel6047

(Ryder, 2004.4.26)

Gene not disrupted in

Df(2L)10523-17

(Marie et al., 2004)

Df(2L)DS9

(Koh et al., 2004)

Df(2L)Rev18

(Koh et al., 2004)

Gene partially disrupted in

Df(2L)Dap160^2543-Δ1

(Marie et al., 2004)

Df(2L)Nhe2^null

(Grillo-Hill et al., 2015)

Inferred from location ( 10 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal

lethal (with Df(2L)bur-K1)

lethal - all die before end of larval stage (with Df(2L)10523-35)

Dap160^Q24

lethal - all die before end of larval stage | recessive

lethal - all die before end of P-stage (with Dap160^Δ1)

Dap160^Δ2

lethal - all die before end of P-stage (with Dap160^Δ2)

Dap160^Δ1

lethal - all die before end of P-stage (with Df(2L)bur-K1)

Dap160^Δ1

lethal - all die before end of P-stage | recessive

partially lethal (with Df(2L)BSC302)

Dap160^{CR00465-TG4.0}

viable

Dap160^EP2543

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

Dap160^GD7159

viable, with Scer\GAL4^Mef2.PR

Dap160^GD7159

viable, with Scer\GAL4^pnr-MD237

Dap160^GD7159

viable, with Scer\GAL4^tin.CΔ4

Dap160^GD7159

Other Phenotypes

Allele

abnormal mitotic cell cycle (with Dap160^Q24)

Dap160^Δ1

abnormal mitotic cell cycle (with Dap160^Δ1)

Dap160^Q24

abnormal neuroanatomy (with Dap160^Δ1)

abnormal neuroanatomy (with Dap160^Δ2)

Dap160^Δ1

abnormal neuroanatomy (with Dap160^Δ3)

Dap160^Δ1

abnormal neuroanatomy | larval stage (with Dap160^EMS)

Dap160^Δ1

abnormal neuroanatomy | larval stage (with Dap160^Δ1)

Dap160^EMS

abnormal neuroanatomy | larval stage (with Df(2L)bur-K1)

Dap160^Δ1

abnormal neuroanatomy | third instar larval stage (with Dap160^EMS1)

Dap160^Δ2

abnormal neuroanatomy | third instar larval stage (with Dap160^Q24)

Dap160^Δ1

abnormal neuroanatomy | third instar larval stage (with Dap160^Δ1)

Dap160^Q24

abnormal neuroanatomy | third instar larval stage (with Dap160^Δ2)

Dap160^EMS1

abnormal neurophysiology

Dap160^Q24

abnormal neurophysiology (with Dap160^Δ1)

abnormal neurophysiology (with Dap160^Δ2)

Dap160^Δ1

abnormal neurophysiology (with Dap160^Δ3)

Dap160^Δ1

abnormal neurophysiology (with Df(2L)10523-35)

Dap160^Q24

abnormal neurophysiology | larval stage (with Df(2L)bur-K1)

Dap160^Δ1

paralytic | heat sensitive (with Dap160^EMS)

Dap160^Δ1

paralytic | heat sensitive (with Dap160^Δ1)

paralytic | heat sensitive (with Dap160^Δ6)

Dap160^Δ1

some die during larval stage (with Df(2L)10523-35)

Dap160^Q24

some die during larval stage | recessive

some die during pharate adult stage (with Dap160^Δ1)

Dap160^Δ2

some die during pharate adult stage (with Dap160^Δ2)

Dap160^Δ1

some die during pharate adult stage (with Df(2L)bur-K1)

Dap160^Δ1

some die during pharate adult stage | recessive

uncoordinated | adult stage (with Dap160^EMS)

Dap160^Δ1

uncoordinated | adult stage (with Dap160^Δ1)

uncoordinated | adult stage (with Dap160^Δ3)

Dap160^Δ1

uncoordinated | adult stage (with Dap160^Δ6)

Dap160^Δ1

wild-type

Dap160^EP2543

Phenotype manifest in

Allele

bouton (with Dap160^Δ1)

bouton (with Dap160^Δ2)

Dap160^Δ1

bouton (with Dap160^Δ3)

Dap160^Δ1

bouton (with Df(2L)bur-K1)

Dap160^Δ1

bouton | increased number

Dap160^Q24

bouton | increased number (with Dap160^Δ1)

bouton | increased number (with Dap160^Δ2)

Dap160^Δ1

bouton | increased number (with Dap160^Δ3)

Dap160^Δ1

bouton | increased number (with Df(2L)10523-35)

Dap160^Q24

embryonic/larval neuromuscular junction (with Df(2L)bur-K1)

Dap160^Δ1

eo neuron | increased number, with Scer\GAL4^sca-537.4

Dap160^UAS.cTa

eo sensory structure, with Scer\GAL4^sca-537.4

Dap160^UAS.cTa

larval neuroblast (with Dap160^Q24)

Dap160^Δ1

larval neuroblast (with Dap160^Δ1)

Dap160^Q24

larval neuroblast & larval brain, with Scer\GAL4^wor.PA

Dap160^UAS.cMa

larval neuroblast & larval brain (with Dap160^Q24)

Dap160^Δ1

larval neuroblast & larval brain (with Dap160^Δ1)

Dap160^Q24

macrochaeta, with Scer\GAL4^sca-537.4

Dap160^UAS.cTa

neuromuscular junction (with Dap160^Δ1)

neuromuscular junction (with Dap160^Δ2)

Dap160^Δ1

neuromuscular junction (with Dap160^Δ3)

Dap160^Δ1

NMJ bouton | increased number (with Dap160^EMS1)

Dap160^Δ2

NMJ bouton | increased number (with Dap160^Δ2)

Dap160^EMS1

NMJ bouton | larval stage | increased number (with Dap160^EMS)

Dap160^Δ1

NMJ bouton | larval stage | increased number (with Dap160^Δ1)

Dap160^EMS

synaptic vesicle (with Dap160^Δ1)

Dap160^Δ2

synaptic vesicle (with Dap160^Δ2)

Dap160^Δ1

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (111)

Hsap\ITSN1

14 of 14

Yes

Hsap\ITSN2

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\NCF1C

1 of 14

Yes

Hsap\NCF1

1 of 14

Yes

Hsap\NCF2

1 of 14

Yes

Hsap\NCF4

1 of 14

Yes

Hsap\NCK1

1 of 14

Hsap\NCK2

1 of 14

Hsap\NOXA1

1 of 14

Yes

Hsap\NOXO1

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Hsap\SH3PXD2B

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (76)

Rnor\Itsn1

14 of 14

Yes

Rnor\Itsn2

12 of 14

Yes

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Ncf4

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Rnor\Sh3pxd2b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Mus musculus (laboratory mouse) (77)

Mmus\Itsn1

14 of 14

Yes

Mmus\Itsn2

12 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Ncf2

1 of 14

Yes

Mmus\Ncf4

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Mmus\Sh3pxd2b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Xenopus tropicalis (Western clawed frog) (62)

Xtro\itsn2

10 of 13

Yes

Xtro\itsn1

7 of 13

Yes

5 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\arhgap4

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Xtro\sla

1 of 13

Yes

Xtro\srgap1

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Danio rerio (Zebrafish) (101)

Drer\itsn1

13 of 14

Yes

Drer\itsn2a

13 of 14

Yes

Drer\itsn2b

13 of 14

Yes

2 of 14

2 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\arhgap4a

1 of 14

Drer\arhgap4b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\ncf2

1 of 14

Yes

Drer\ncf4

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\noxo1b

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Drer\sh3pxd2ab

1 of 14

Yes

Drer\sh3pxd2b

1 of 14

Yes

Drer\sh3rf1

1 of 14

Drer\sh3rf2

1 of 14

Drer\sh3yl1

1 of 14

Yes

Drer\si:ch211-51c14.1

1 of 14

Drer\si:ch211-105f12.2

1 of 14

Drer\si:ch211-176g13.8

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (26)

Cele\itsn-1

13 of 14

Yes

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Yes

Anopheles gambiae (African malaria mosquito) (31)

Agam\AgaP_AGAP009037

12 of 12

Yes

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (11)

Atha\AT1G20760

3 of 13

Yes

Atha\AT1G21630

3 of 13

Yes

2 of 13

1 of 13

1 of 13

1 of 13

Yes

Atha\AT4G18060

1 of 13

Atha\AT4G39020

1 of 13

Yes

Atha\AT5G05180

1 of 13

Yes

Atha\NET4A

1 of 13

Yes

Atha\NET4B

1 of 13

Yes

Saccharomyces cerevisiae (Brewer's yeast) (12)

Scer\EDE1

3 of 13

Yes

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Scer\USO1

1 of 13

Schizosaccharomyces pombe (Fission yeast) (13)

Spom\SPBC800.10c

4 of 12

Yes

Spom\ucp8

4 of 12

Yes

2 of 12

2 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

1 of 12

Yes

Spom\SPBC4F6.14

1 of 12

Spom\SPBC19C2.10

1 of 12

Spom\SPBC119.05c

1 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:Dap160. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (27)

4 of 13

4 of 13

3 of 13

2 of 13

tmn

2 of 13

Abl

1 of 13

Btk

1 of 13

CAP

1 of 13

CG4806

1 of 13

CG33228

1 of 13

CG34214

1 of 13

Crk

1 of 13

dock

1 of 13

drk

1 of 13

EndoA

1 of 13

EndoB

1 of 13

Nost

1 of 13

nwk

1 of 13

Pi3K21B

1 of 13

POSH

1 of 13

Rbp

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

vap

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Down syndrome, ITSN1-SYNJ1-RCAN1-related

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 2 )

Allele

Disease

Evidence

References

Dap160^UAS.cMa

model of Down syndrome

CEA with sra^UAS.Tag:HA, Synj^+tKa

(Chang and Min, 2009)

Dap160^JF01918

model of intellectual disability

CEA

(Haddadi and Ataei, 2022)

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 5 )

Allele

Disease

Interaction

References

Dap160^Δ1

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

exacerbates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Spargo et al., 2025)

Dap160^Δ2

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Dap160^EP2543

ameliorates amyotrophic lateral sclerosis type 8

modeled by Vap33^{P58S.UAS.Tag:FLAG}

(Sanhueza et al., 2015)

Dap160^UAS.cKa

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cJa

(Spargo et al., 2025)

Dap160^KK102297

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

14 of 14

13 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

Dap160 - Cbl

anti bait coimmunoprecipitation, western blot, pull down, autoradiography

(Robertson et al., 2000)

Dap160 - Chc

far western blotting, molecular weight estimation by staining

(Roos and Kelly, 1998)

Dap160 - Eps-15

anti bait coimmunoprecipitation, peptide massfingerprinting, western blot

(Winther et al., 2015, Koh et al., 2007)

Dap160 - Snap29

pull down, western blot

(Xu et al., 2014)

Dap160 - Syn

anti bait coimmunoprecipitation, western blot, far western blotting, molecular weight estimation by staining, pull down

(Winther et al., 2015, Roos and Kelly, 1998)

Dap160 - Synj

pull down, western blot, anti bait coimmunoprecipitation, far western blotting, molecular weight estimation by staining

(Chen et al., 2014, Verstreken et al., 2003, Roos and Kelly, 1998)

Dap160 - Syt1

cosedimentation, western blot

(Kelly and Phillips, 2005)

Dap160 - aPKC

anti bait coimmunoprecipitation, western blot, Identification by mass spectrometry, pull down, autoradiography

(Chabu and Doe, 2008)

Dap160 - hpo

anti tag coimmunoprecipitation, peptide massfingerprinting

(Kwon et al., 2013)

Dap160 - numb

pull down, western blot

(Tang et al., 2005)

Dap160 - nwk

two hybrid, pull down, western blot, molecular weight estimation by staining

(Del Signore et al., 2021, O'Connor-Giles et al., 2008, Rodal et al., 2008)

Dap160 - par-6

pull down, autoradiography

(Chabu and Doe, 2008)

Dap160 - shi

far western blotting, molecular weight estimation by staining, pull down, western blot, cosedimentation, anti bait coimmunoprecipitation, autoradiography, edman degradation

(Winther et al., 2015, Chen et al., 2014, Winther et al., 2013, Rodal et al., 2008, Kelly and Phillips, 2005, Verstreken et al., 2003, Razzaq et al., 2001, Robertson et al., 2000, Roos and Kelly, 1998)

Dap160 - stnB

anti bait coimmunoprecipitation, western blot, pull down, far western blotting, anti tag western blot, cosedimentation

(Kelly and Phillips, 2005)

Summary of Genetic Interactions

Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Dap160

suppressible

wit

(O'Connor-Giles et al., 2008)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Cbl

enhanceable

Dap160

(Robertson et al., 2000)

Dad

suppressible

Dap160

(O'Connor-Giles et al., 2008)

Nf1

suppressible

Dap160

(Walker et al., 2013)

nwk

enhanceable

Dap160

(O'Connor-Giles et al., 2008)

tkv

suppressible

Dap160

(O'Connor-Giles et al., 2008)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

61333

DroID - A comprehensive database of gene and protein interactions.

FBgn0023388

MIST (protein-protein) - An integrated Molecular Interaction Database

35378

Pathways

Signaling Pathways (FlyBase)

NEGATIVE REGULATORS OF NOTCH SIGNALING PATHWAY

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O61618

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

39A3-39A4

Sequence location

2L:21,134,439..21,142,347 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

39A3-39A4

; Limits computationally determined from genome sequence between P{PZ}l(2)05287⁰⁵²⁸⁷ and P{lacW}l(2)k07215^k07215&P{lacW}snRNA:U4:39B^k09410

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

39A-39A

(Lloyd et al., 2000)

Determined by comparing Celera genomic sequence with sequence from BDGP BAC and P1 clones.

(Lloyd et al., 2000)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-54.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (12)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

19582

w¹¹¹⁸; P{EP}Dap160^EP2543

25879

y¹ v¹; P{TRiP.JF01918}attP2

42695

y¹ w^*; L²/CyO; P{UAS-Dap160.K}4.1

24877

w^*; Dap160^Δ1/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

24878

w^*; Dap160^Δ2/CyO, P{GAL4-Kr.C}DC3, P{UAS-GFP.S65T}DC7

78917

y¹ w^*; TI{CRIMIC.TG4.0}Dap160^{CR00465-TG4.0}/SM6a

605365

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{Car20y}25F Dap160^EMS P{neoFRT}40A

NIG-Fly

M2L-0327

y¹ w¹¹¹⁸; Dap160^SK4 P{neoFRT}40A/CyO-Tb

M2L-0328

y¹ w¹¹¹⁸; Dap160^SK7 P{neoFRT}40A/CyO-Tb

2LG-0258

y² VhaAC39-1^cho-1 v¹; P{U6-Dap160.NIG.sgRNA}attP40/CyO

VDRC

v106689

P{KK102297}VIE-260B

v16158

w¹¹¹⁸; P{GD7159}v16158

Genomic Clones (19)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (53)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

IP14822

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0023388

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0023388

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Chabu and Doe, 2008, Roos and Kelly, 1998)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Dap160 is required for synaptic development and vesicle endocytosis.

(Koh et al., 2004)

Mutant neuromuscular junctions show a mild defect in synaptic vesicle endocytosis at 22^oC, which is strongly enhanced at 34^oC.

(Koh et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: Binding partner of the shi gene product, dynamin.

(Roos and Kelly, 1997)

Relationship to Other Genes

Source for database merge of

Additional comments

Nomenclature History

Source for database identify of

Source for identity of: Dap160 CG1099

(Wortman, 1999.11)

Nomenclature comments

Etymology

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

CG1099

(National Institute of Genetics Fly Stocks, 2022-, Kondo et al., 2017, Neuert et al., 2017, Harris et al., 2016, Rosa-Ferreira et al., 2015, Howell et al., 2012, Cammarato et al., 2011, Khanna et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Giannakou and Dow, 2001, Verstreken and Bellen, 2000.9.4)

DAP-160

(Kelly and Phillips, 2005, Lloyd et al., 2000)

DAP160

(Coyle, 2014, Podufall et al., 2014, Xu et al., 2014, Beck et al., 2012, Phillips et al., 2010, Mohrmann et al., 2008, Bao et al., 2007, Razzaq et al., 2001, Chang and Balice-Gordon, 2000, Roos et al., 2000)

Dap160

(Zheng et al., 2023, National Institute of Genetics Fly Stocks, 2022-, Uçkun et al., 2021, Hope et al., 2020, Hall et al., 2019, Schroeder et al., 2019, Gene Disruption Project members, 2018-, Lee et al., 2018, Kondo et al., 2017, Neuert et al., 2017, Chen et al., 2015, Kavi et al., 2015, Kelley et al., 2015, Sanhueza et al., 2015, Chen et al., 2014, Mukherjee et al., 2014, Kwon et al., 2013, Kwon et al., 2013, Maldonado et al., 2013, Walker et al., 2013, Winther et al., 2013, Howell et al., 2012, Cheng et al., 2011, Kawasaki et al., 2011, Chang et al., 2010, Jiao et al., 2010, Li et al., 2010, Schulte et al., 2010, Johnson et al., 2009, Massaro et al., 2009, Venken et al., 2009, Verstreken et al., 2009, Rodal et al., 2008, Buszczak et al., 2007, Chabu et al., 2007, Korolchuk et al., 2007, Scappini et al., 2007, Kittel et al., 2006, Bao et al., 2005, Tang et al., 2005, Robertson et al., 2000)

Dap160/intersectin

(Götz et al., 2021, Narayanan and Ramaswami, 2001)

noncoding_4110

(FlyBase Genome Annotators, 2011, The modENCODE Consortium, 2010)

p160

(Roos and Kelly, 1997)

Name Synonyms

Dynamin associated protein 160

(Cammarato et al., 2011, Khanna et al., 2010, Roos and Kelly, 1997)

Dynamin-associated protein 160 kDa

(Winther et al., 2013)

Intersectin

(Johnson et al., 2009, Verstreken and Bellen, 2006)

dynamin associated protein 160

(Gupta et al., 2014)

intersectin

(Winther et al., 2013, Kawasaki et al., 2011)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 77 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35378

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

M9ND00

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

M9ND00

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0023388

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

35378/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0023388

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0023388

FlyMine - An integrated database for Drosophila genomics

FBgn0023388

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG1099

MARRVEL_MODEL - MARRVEL (model organism gene)

35378

Linkouts

BioGRID - A database of protein and genetic interactions.

61333

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0023388

DroID - A comprehensive database of gene and protein interactions.

FBgn0023388

DRSC - Results frm RNAi screens

FBgn0023388

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Dap160_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0023388

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0023388

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1099

Flygut - An atlas of the Drosophila adult midgut

FBgn0023388

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0023388

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC032394

MIST (protein-protein) - An integrated Molecular Interaction Database

35378

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O61618

References (146)

The following external sites may use different assemblies or annotations than FlyBase.

0.92

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