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Gene Dmel\mip130

General Information
Symbol	Dmel\mip130	Species	D. melanogaster
Name	Myb-interacting protein 130	Annotation symbol	CG3480
Feature type	protein_coding_gene	FlyBase ID	FBgn0023509
Gene Model Status	Current	Stock availability	3 publicly available
Also Known As	TWIT, EG:86E4.4
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	2B15-2B16	Sequence location	X:1,802,246..1,814,351 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Myb-interacting protein 130 is referred to in FlyBase by the symbol Dmel\mip130 (CG3480, FBgn0023509). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: DNA binding. There is experimental evidence that it is involved in the biological process: centrosome organization; mitotic spindle organization; negative regulation of transcription from RNA polymerase II promoter. 15 alleles are reported. The phenotype of these alleles is annotated with: chorion. It has one annotated transcript and one annotated polypeptide. Protein features are: DIRP. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. Comments on Affy2 ProbeSet: ProbeSet 1639185_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of mip130. Gene sequence location is X:1802246..1814351.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2011_10	References Knowles-Barley, 2011.8.24, BrainTrap expression curation.
FB2012_01	Sequence features BKN21848 HFA18575
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 2B15-2B16   Limits computationally determined from genome sequence between P{EP}EP1444&P{EP}CG14818EP1190 and P{EP}CG3600EP1232
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\mip130 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) mip130-RA FBtr0070315  NM_130602   3485   986
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein mip130-PA   FBpp0070302   110.4   986   4.93     NP_569958   AAF45694
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • DIRP (IPR010561)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202501   AA246264   AA390321   AA392701   AA441651   AA539616   AA941612   AA941864   AA948894   AA979426   AE014298 AAF45694   AI259343   AI456549   AI516467   AI516694   AI520539   AL021086 CAA15930   AY819021 AAV68084   BG638148   BG638807   BI162225   BI171619   BI172680   BI375408   BI577672   BI577960   BI580625   BI632327   BT021408 AAX33556   CO267777   CO278945   CO280768   CO297005   CO312300   CO316755   CO339365   EC052576   EC061233   EC076184   EC084690   EC087289   EC233861   EC256678   EL886601   EL886652   ES696433   GH752252   GH757243   GH758847   GH759855   GH761193   GH774662   GH778327   GH789804   GH796880   GH802452   GH809396   GH809847   GH811932   GH815284   GH820331   GH820817   GH824620   GH826314   GH830454   GH831776   GH842232   GH844220   GH883857   GH889125   GH889278   GH892634   GH896815   GH899239   GH899299   GH900567   GH907634   GH913455   GH915273   GH919920   GH928769   GH937094   GH984927
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O46093 Q9W542
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage   organism   (Beall et al., 2004)
Additional Descriptive Data
Marker for
	mip130
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0023509    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0023509 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-18 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0023509 expression level Developmental Stage   Expression Level embryo 00-02hr    2222 embryo 02-04hr    3352 embryo 04-06hr    3523 embryo 06-08hr    3818 embryo 08-10hr    3329 embryo 10-12hr    2229 embryo 12-14hr    1728 embryo 14-16hr    1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day    1431 adult female 30day    1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2222) embryo 02-04hr  (3352) embryo 04-06hr  (3523) embryo 06-08hr  (3818) embryo 08-10hr  (3329) embryo 10-12hr  (2229) embryo 12-14hr  (1728) embryo 14-16hr  (1580) embryo 16-18hr  (1171) embryo 18-20hr  (888) embryo 20-22hr  (1367) embryo 22-24hr  (952) larva L1  (702) larva L2  (620) larva L3 12hr old  (541) larva L3 puffstage 1-2  (480) larva L3 puffstage 3-6  (820) larva L3 puffstage 7-9  (918) white prepupae new  (957) white prepupae 12hr  (1048) white prepupae 24hr  (1236) pupae 2d postWPP  (703) pupae 3d postWPP  (419) pupae 4d postWPP  (378) adult male 01day  (438) adult male 05day  (439) adult male 30day  (414) adult female 01day  (1133) adult female 05day  (1431) adult female 30day  (1467) Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (2222) embryo 02-04hr  (3352) embryo 04-06hr  (3523) embryo 06-08hr  (3818) embryo 08-10hr  (3329) embryo 10-12hr  (2229) embryo 12-14hr  (1728) embryo 14-16hr  (1580) embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day  1431 adult female 30day  1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2222 embryo 02-04hr    3352 embryo 04-06hr    3523 embryo 06-08hr    3818 embryo 08-10hr    3329 embryo 10-12hr    2229 embryo 12-14hr    1728 embryo 14-16hr    1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day    1431 adult female 30day    1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2222 embryo 02-04hr    3352 embryo 04-06hr    3523 embryo 06-08hr    3818 embryo 08-10hr    3329 embryo 10-12hr    2229 embryo 12-14hr    1728 embryo 14-16hr    1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day    1431 adult female 30day    1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0023509 expression level Developmental Stage   Expression Level embryo 00-02hr    2222 embryo 02-04hr    3352 embryo 04-06hr    3523 embryo 06-08hr    3818 embryo 08-10hr    3329 embryo 10-12hr    2229 embryo 12-14hr    1728 embryo 14-16hr    1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day    1431 adult female 30day    1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr  (2222) embryo 02-04hr  (3352) embryo 04-06hr  (3523) embryo 06-08hr  (3818) embryo 08-10hr  (3329) embryo 10-12hr  (2229) embryo 12-14hr  (1728) embryo 14-16hr  (1580) embryo 16-18hr  (1171) embryo 18-20hr  (888) embryo 20-22hr  (1367) embryo 22-24hr  (952) larva L1  (702) larva L2  (620) larva L3 12hr old  (541) larva L3 puffstage 1-2  (480) larva L3 puffstage 3-6  (820) larva L3 puffstage 7-9  (918) white prepupae new  (957) white prepupae 12hr  (1048) white prepupae 24hr  (1236) pupae 2d postWPP  (703) pupae 3d postWPP  (419) pupae 4d postWPP  (378) adult male 01day  (438) adult male 05day  (439) adult male 30day  (414) adult female 01day  (1133) adult female 05day  (1431) adult female 30day  (1467) Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  2222 embryo 02-04hr  (3352) embryo 04-06hr  (3523) embryo 06-08hr  (3818) embryo 08-10hr  (3329) embryo 10-12hr  2229 embryo 12-14hr  1728 embryo 14-16hr  1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day  1431 adult female 30day  1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    2222 embryo 02-04hr    3352 embryo 04-06hr    3523 embryo 06-08hr    3818 embryo 08-10hr    3329 embryo 10-12hr    2229 embryo 12-14hr    1728 embryo 14-16hr    1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day    1431 adult female 30day    1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    2222 embryo 02-04hr    3352 embryo 04-06hr    3523 embryo 06-08hr    3818 embryo 08-10hr    3329 embryo 10-12hr    2229 embryo 12-14hr    1728 embryo 14-16hr    1580 embryo 16-18hr    1171 embryo 18-20hr    888 embryo 20-22hr    1367 embryo 22-24hr    952 larva L1    702 larva L2    620 larva L3 12hr old    541 larva L3 puffstage 1-2    480 larva L3 puffstage 3-6    820 larva L3 puffstage 7-9    918 white prepupae new    957 white prepupae 12hr    1048 white prepupae 24hr    1236 pupae 2d postWPP    703 pupae 3d postWPP    419 pupae 4d postWPP    378 adult male 01day    438 adult male 05day    439 adult male 30day    414 adult female 01day    1133 adult female 05day    1431 adult female 30day    1467 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0023509    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0023509 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0023509 expression level Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0023509 expression level Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    101.375 Larval Midgut    20.8 Larval Hindgut    38.8 Larval Malpighian Tubules    42.7 Larval Fat Body    26 Larval Salivary Gland    64.8 Larval Trachea    66.425 Larval Carcass    39.575 Adult Head    29 Adult Eye    34.05 Adult Brain    55 Adult Thoracic-Abdominal Ganglion    33.9 Adult Crop    36 Adult Midgut    22 Adult Hindgut    28.7 Adult Malpighian Tubules    27.9 Adult Fat Body    33.8 Adult Salivary Gland    55.6 Adult Heart    27.35 Adult VirginFemale Spermatheca    17.9 Adult InseminatedFemale Spermatheca    20 Adult Ovary    83.2 Adult Testis no informative data Adult Male Accessory Gland    40.5 Adult Carcass    33.2 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis no informative data Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    29   NA  Eye    34.05   NA  Brain    55   101.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    33.9   NA  Crop    36   20.8  Midgut    22   38.8  Hindgut    28.7   42.7  Malpighian Tubules    27.9   26  Fat Body    33.8   64.8  Salivary Gland    55.6   NA  Heart    27.35   66.425  Trachea    NA   NA  VirginFemale Spermatheca    17.9   NA  InseminatedFemale Spermatheca    20   NA  Ovary    83.2   NA  Testis    no informative data   NA  Male Accessory Gland    40.5   39.575  Carcass    33.2 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_17
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023509 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3480-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele semi-viable mip1301-723 viable mip1301-36 viable (with mip1301-723+1-36.3') mip1301-723+1-36.5' viable (with mip1301-723+1-36.5') mip1301-723+1-36.3' Sterility Allele female semi-sterile mip1301-36 female sterile mip130unspecified female sterile (with mip1301-723+1-36.3') mip1301-723+1-36.5' female sterile (with mip1301-723+1-36.5') mip1301-723+1-36.3' Other Phenotypes Allele small body mip1301-723 small body (with mip1301-723+1-36.3') mip1301-723+1-36.5' small body (with mip1301-723+1-36.5') mip1301-723+1-36.3' Phenotype manifest in Allele chorion mip1301-36 chorion (with mip1301-723+1-36.3') mip1301-723+1-36.5' chorion (with mip1301-723+1-36.5') mip1301-723+1-36.3'
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of mip130 Class Mutagen Stocks Known lesion mip130G551 P-element activity 1 -- mip1301-36 loss of function allele I-CreI endonuclease 0 Yes mip1301-723+1-36.3' SCEI endonuclease FLPase 0 Yes mip1301-723+1-36.5' FLPase SCEI endonuclease 0 Yes mip1301-723 neomorphic allele - genetic evidence I-CreI endonuclease 0 Yes mip130CPTI003151 piggyBac transposase 0 Yes mip130unspecified 0 --
Alleles Carried on Transgenic Constructs ( 8 )
For All Alleles Carried on Transgenic Constructs Show
Allele of mip130 Class Mutagen Stocks Known lesion mip130GD10978 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mip130HMS00462 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes mip130dsRNA.cBa in vitro construct - RNAi 0 Yes mip130dsRNA.cKa in vitro construct - RNAi 0 Yes mip130KK101797 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes mip130MtnA.C.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mip130MtnA.N.T:Avic\GFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes mip130T:Zzzz\FLAG in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD10978} NA P{KK101797} NA P{TRiP.HMS00462} NA characterization construct P{mip130-Flag} NA
Insertions
	Type of insertions Name Expression data insertion of enhancer trap P{lacW}l(1)G0220G0220 No insertion of mobile activating element P{EP}Rbcn-3BG551 NA miscellaneous insertions PBac{754.P.FSVS-0}mip130CPTI003151 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 7 terms )
Molecular Function
CV term Evidence References
DNA binding inferred from direct assay (Beall et al., 2002)
Biological Process
CV term Evidence References
centrosome organization inferred from mutant phenotype (Goshima et al., 2007) mitotic spindle organization inferred from mutant phenotype (Goshima et al., 2007) negative regulation of transcription from RNA polymerase II promoter inferred from mutant phenotype (Lewis et al., 2004, Korenjak et al., 2004)
Cellular Component
CV term Evidence References
chromatin inferred from direct assay (Korenjak et al., 2004) Myb complex inferred from direct assay (Beall et al., 2002, Korenjak et al., 2004) polytene chromosome inferred from direct assay (Beall et al., 2007)
Terms Based on Predictions or Assertions ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
eggshell chorion gene amplification inferred by curator from GO:0031523 (Beall et al., 2002)
Cellular Component
CV term Evidence References
cytoplasm non-traceable author statement (Begum and White-Cooper, 2003, Begum and White-Cooper, 2003) nucleus inferred from sequence or structural similarity (White-Cooper et al., 2000) non-traceable author statement (Begum and White-Cooper, 2003, Begum and White-Cooper, 2003)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions mip130 allele Gene References mip1301-36 Myb (Beall et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57699 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0023509 DroID - A comprehensive database of gene and protein interactions. • FBgn0023509 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31153
Orthologs
Genome-wide drosophilid orthologs	Dana\GF19452 Dere\GG12891 Dgri\GH11979 Dmoj\GI14720 Dper\GL18218 Dpse\GA27784 Dsec\GM19180 Dsim\GD24729 Dvir\GJ16785 Dwil\GK16330 Dyak\GE16223
Curated drosophilid orthologs	Dpse\GA17473
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • AAEL005631-PA Aedes aegypti (mosquito) AGAP004624-PA Anopheles gambiae (mosquito) XP_394339.2 Apis mellifera (honey bee) XP_001949408.1 Acyrthosiphon pisum (pea aphid) At3g05380.1 Arabidopsis thaliana (wall cress) 96604 Branchiostoma floridae (lancelet) BGIBMGA001154-PA Bombyx mori (silk worm) CE28195 Caenorhabditis elegans (worm) JA12739 Caenorhabditis japonica (nematode) CPIJ012736 Culex pipens (mosquito) 99454 Capitella spl (polychaete worm) DDB0232078 Dictyostelium discoideum (amoeba) 300555 Daphnia pulex (daphnia) ENSDARP00000045184 Danio rerio (zebra fish) ENSGACP00000014995 Gymnopilus aculeatus (mushroom) ENSP00000355772 Homo sapiens (human) ISCW005548-PA Ixodes scapularis (deer tick) 167874 Nematostella vectensis (starlet anemone) XP_001605183.1 Nasonia vitripennis (wasp) ENSORLP00000017237 Oryzias latipes (japanese killifish) PHUM004416-PA Pediculus humanus (human body louse) ENSPPYP00000000163 Pongo pygmaeus (orangutan) Smp_055340.1 Schistosoma mansoni (trematode parasite) XP_975203.2 Tribolium castaneum (red flour beetle) ENSXETP00000048950 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV14437 LH19032 ISCW005548 FBgn0233763 FBgn0218332 FBgn0196050 FBgn0174082 JGI_V11_300555 FBgn0249148 FBgn0155820 FBgn0137471 FBgn0119458 FBgn0105164 FBgn0096461 CPIJ012736 BGIBMGA001154 ACYPI005224 GB16458 AGAP004624 AAEL005631 PB26051 PHUM614730 GLEAN_08121
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	33260 P{EP}Rbcn-3BG551 mip130G551 w* 32462 y1 sc* v1; P{TRiP.HMS00462}attP2/TM3, Sb1
VDRC	v26205 w1118; P{GD10978}v26205/TM3
Genomic Clones ( 2 )
	BACR05N09 BACR33I18 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 79 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD07342
Other clones	DPiM_LD07342
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD01995 LD03263 LD06046 LD11804 LD16653 LD17562 LD17854 LD25839 LD27175 LD27573 LD33983 LD40730 LD42344 LD42671 LP02735 RE01602 RE13433 RE15325 RE63205 RE71383 RE71740 RE74841 SD26079
Other clones	19254 38912 41489 82153 101475 214213.47 EC052576 EK132362 EK151820 EK154818 EK215721 EK228349 EK265803 EP01727 IP17860 LD36606 RC0-DP0003-150700-021-C03 RP003008185 RP003012996 RP003027669 RP003068457 RP003082531 RP003116881 RP003124222 RP003138270 RP003138783 RP003156501 RP003217290 RP003231705 RP003233326 RP003239519 RP003242389 RP003266831 RP003290548 RP003294165 RP003305964 RP003313210 RP003334501 RP003369999 RP003375360 RP003379917 RP003431452 RP003471360 RP003482824 RP003549210 RP003560284 RP003563216 RP003563462 RP003583352 RP003584181 RP003602602 RP003662129 RP003663989 RP004500477
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0023509 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31153
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments	"l(1)G0220" may affect "CG17766" or "twit". (Peter et al., 2002)
Other Comments
	RNAi screen using dsRNA made from templates generated with primers directed against this gene results in the formation of monastral bipolar spindles when assayed in S2 cells. This phenotype can be observed when the screen is performed with or without Cdc27 dsRNA. (Goshima et al., 2007) RNAi screen using dsRNA made from templates generated with primers directed against this gene in S2 cells does not affect the number of prophase centrosomes, but monastral bipolar spindles are formed during mitosis, suggesting that the centrosomes are fused. (Goshima et al., 2007)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_130602 RefSeq (Proteins) • NP_569958 DDBJ / EMBL / GenBank • AA948894 AAF45694 AAV68084 AAX33556 AL021086 AY819021 BT021408 CAA15930 Entrez Gene - A searchable database of RefSeq genes. • 31153 UniProtKB/TrEMBL • O46093 Q9W542
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • DIRP (IPR010561)
Linkouts
	BioGRID - A database of protein and genetic interactions • 57699 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0023509 DroID - A comprehensive database of gene and protein interactions. • FBgn0023509 DRSC - Results from RNAi screens. • FBgn0023509 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0023509 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG3480-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0023509 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31153 InParanoid A subset of ortholog calls from InParanoid. • AAEL005631-PA Aedes aegypti (mosquito) AGAP004624-PA Anopheles gambiae (mosquito) XP_394339.2 Apis mellifera (honey bee) XP_001949408.1 Acyrthosiphon pisum (pea aphid) At3g05380.1 Arabidopsis thaliana (wall cress) 96604 Branchiostoma floridae (lancelet) BGIBMGA001154-PA Bombyx mori (silk worm) CE28195 Caenorhabditis elegans (worm) JA12739 Caenorhabditis japonica (nematode) CPIJ012736 Culex pipens (mosquito) 99454 Capitella spl (polychaete worm) DDB0232078 Dictyostelium discoideum (amoeba) 300555 Daphnia pulex (daphnia) ENSDARP00000045184 Danio rerio (zebra fish) ENSGACP00000014995 Gymnopilus aculeatus (mushroom) ENSP00000355772 Homo sapiens (human) ISCW005548-PA Ixodes scapularis (deer tick) 167874 Nematostella vectensis (starlet anemone) XP_001605183.1 Nasonia vitripennis (wasp) ENSORLP00000017237 Oryzias latipes (japanese killifish) PHUM004416-PA Pediculus humanus (human body louse) ENSPPYP00000000163 Pongo pygmaeus (orangutan) Smp_055340.1 Schistosoma mansoni (trematode parasite) XP_975203.2 Tribolium castaneum (red flour beetle) ENSXETP00000048950 Xenopus tropicalis (frog) InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31153 modMine - Data generated by the modENCODE project. • FBgn0023509 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • NV14437 LH19032 ISCW005548 FBgn0233763 FBgn0218332 FBgn0196050 FBgn0174082 JGI_V11_300555 FBgn0249148 FBgn0155820 FBgn0137471 FBgn0119458 FBgn0105164 FBgn0096461 CPIJ012736 BGIBMGA001154 ACYPI005224 GB16458 AGAP004624 AAEL005631 PB26051 PHUM614730 GLEAN_08121
Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	86E4.4 (White-Cooper et al., 2000) CG3480 (Beall et al., 2002, Benos, 2000.12.13, Goshima et al., 2007, Beall et al., 2007, Ni et al., 2010.12.1, Keleman et al., 2009.8.5) EG:86E4.4 (Peter et al., 2002, Benos et al., 2001, Giot et al., 2003, ) Mip130 (Korenjak et al., 2004, Tower, 2004, Goshima et al., 2007, Wen et al., 2008, Ullah et al., 2007, Georlette et al., 2007, Wen et al., 2008) mip130 (Doggett et al., 2007, Beall et al., 2007, Scaria et al., 2008, Xi et al., 2008) Mip130/TWIT (Korenjak and Brehm, 2005) TWIT (Korenjak et al., 2004) twit (Begum and White-Cooper, 2003, Begum and White-Cooper, 2003, ) Twit (Goshima et al., 2007, Goshima et al., 2007)
Name Synonym	Myb-interacting protein 130 (Beall et al., 2004) twilight (Begum and White-Cooper, 2003)
Secondary FlyBase IDs
References ( 36 )
Generate a list of	All references relating to this gene ( 36 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 4 ) Review  ( 4 ) Personal communication to FlyBase  ( 7 ) Abstract  ( 4 ) FlyBase analysis  ( 1 ) Computer file  ( 1 )
Recent research papers (0)
	All research papers listed in FlyBase were published before 2010 Show research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...