A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Gene Dmel\zpg

General Information
SymbolDmel\zpgSpeciesD. melanogaster
Namezero population growthAnnotation symbolCG10125
Feature typeprotein_coding_geneFlyBase IDFBgn0024177
Created / Updated2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)3LRecombination map
Cytogenetic map 65B5-65B5 Sequence location3L:6,597,455..6,599,129 [-]
Map ( GBrowse ) detailed view
hide Summary Information
Automatically generated summary

See sections below for more information
The gene zero population growth is referred to in FlyBase by the symbol zpg (CG10125, FBgn0024177). It has the cytological map location 65B5. Its sequence location is 3L:6597455..6599129. Its molecular function is described as gap junction channel activity. It is involved in the biological process germ cell development. 14 alleles are reported. The phenotypes of these alleles are annotated with 12 unique terms, many of which group under: organ system; anatomical structure; germarium; organism; portion of tissue; spermatogonium; hub cell; cyst cell; germ cell; developing anatomical structure. It has one annotated transcript and one annotated polypeptide.

External Summaries

hide Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
65B5-65B5  
Limits computationally determined from genome sequence between P{PZ}l(3)0209402094 and P{lacW}l(3)L4060L4060  
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
Experimentally Determined Recombination Data
Location
Left of (cM)
(Tazuke et al., 2002)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology)
References
In direction of increasing cytology: ndl- CG8368+ zpg- CG8368+
(Tazuke et al., 2002)
Gene Order (overall orientation not stated)
References
hide Gene Model & Products
Please see the GBrowse view of Dmel\zpg for information on other features
detailed view FBtr0076984 FBtr0076976 FBtr0076983 FBtr0076977 FBpp0076693 FBpp0076692 FBpp0076686 FBpp0076685 FBti0070984 FBti0023809 FBti0044859
Comments on Gene Model
hide Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Associated CDS (aa)
zpg-RA
FBtr0076983
 NM_139792
  1547
  367
Additional Transcript Data & Comments
Reported size (kB)
1.6 (northern blot)
(Tazuke et al., 2002)
Comments
External Data
Crossreferences
hide Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
zpg-PA  
FBpp0076692  
42.8  
367  
9.18  
  NP_648049
  AAF50655
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
PANTHER - Protein classification by function, families, and pathways
•
Crossreferences
InterPro domains - A database of protein families, domains, and functional sites
•
Innexin (IPR000990)
hide Sequences Consistent with the Gene Model
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
AA801795
 
AE003562
AAF50655
 
AF271718
AAL36976
 
AI513218
 
AY094856
AAM11209
 
BI213186
 
BI374380
 
BI482285
 
BI483848
 
BI483885
 
BP549243
 
BP549244
 
CG10125
 
CO278507
 
CO300357
 
CO322757
 
CO326833
 
DU708657
 
EC063108
 
EC233157
 
EC245815
 
EC246161
 
EC257994
 
 
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
    Maps to
    Does NOT map to
    Identified with
    (Stebbings et al., 2002)
    (BDGP Project Members, 2000-)
    (Todman, 2000.5.22)
    hide Mapped Features & Mutations
    Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here
    Type
    Symbol & Location
    Additional Notes
    References
    point mutation
    zpg[1]
    3L:6,598,948..6,598,948
    evidence=experimental
    na_change=A6579795T
    pr_change=D21V|zpg-PA
    reported_na_change=A150T
    reported_pr_change=D21V
    (Tazuke et al., 2002)
    point mutation
    zpg[z-5352]
    3L:6,598,739..6,598,739
    evidence=experimental
    na_change=C6579586T
    pr_change=R91@|zpg-PA
    reported_na_change=C359T
    reported_pr_change=R91@
    point mutation
    zpg[z-2679]
    3L:6,598,677..6,598,677
    comment=reported as mutation in splice site acceptor site between amino acid residues 92/93
    evidence=experimental
    na_change=G6579524A
    reported_na_change=G366A
    (Tazuke et al., 2002)
    point mutation
    zpg[2]
    3L:6,598,179..6,598,179
    evidence=experimental
    na_change=C6579026T
    pr_change=P235S|zpg-PA
    reported_na_change=C919T
    reported_pr_change=P235S
    (Tazuke et al., 2002)
    point mutation
    zpg[z-0918]
    3L:6,598,172..6,598,172
    evidence=experimental
    na_change=G6579019A
    pr_change=C237Y|zpg-PA
    reported_na_change=G926A
    reported_pr_change=C237Y
    (Tazuke et al., 2002)
    point mutation
    zpg[z-2552]
    3L:6,598,167..6,598,167
    evidence=experimental
    na_change=A6579014T
    pr_change=K239@|zpg-PA
    reported_na_change=A931T
    reported_pr_change=K239@
    (Tazuke et al., 2002)
    point mutation
    zpg[z-2533]
    3L:6,598,167..6,598,167
    evidence=experimental
    na_change=A6579014T
    pr_change=K239@|zpg-PA
    reported_na_change=A931T
    reported_pr_change=K239@
    (Tazuke et al., 2002)
    point mutation
    zpg[z-3860]
    3L:6,598,055..6,598,055
    na_change=G6578902A
    pr_change=W276@|zpg-PA
    reported_na_change=G1043A
    reported_pr_change=W276@
    evidence=experimental
    (Tazuke et al., 2002)
    rescue fragment
    zpg[+t6.15]
    3L:6,595,302..6,601,443
    comment=Authors report rescue by a 6.15 kb Bam HI-Hind III fragment obtained from a genomic phage clone. The indicated Bam HI fragment is of the correct size and position based on the map presented in figure 4.
    linked_to=BamHI-BamHI_rfrag
    (Tazuke et al., 2002)
    hide External Data
    Linkouts
    DEDB - Drosophila exon database: splicing graphs
    •
    Crossreferences
    hide Expression Data
    FlyBase-Curated Data
    Transcript and
    Protein data     		Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
    hide Summary of Transcript Expression
    Stage
    Tissue/Position
    Reference
     embryonic stage | contracted germ band stage
     germ cell
    (Tazuke et al., 2002)
     embryonic stage | blastoderm
     pole cell
    (Tazuke et al., 2002)
     adult stage
     germ cell
    (Tazuke et al., 2002)
     adult stage | male
     spermatogonium
    (Tazuke et al., 2002)
    Marker for
      Subcellular Localization
      CV Term
      hide Summary of Polypeptide Expression
      Stage
      Tissue/Position
      Reference
       adult stage
       egg chamber stage 6-7
      (Tazuke et al., 2002)
       larval stage | third instar
       spermatogonium
      (Tazuke et al., 2002)
       adult stage
       germarium
      (Tazuke et al., 2002)
       adult stage
       ovariole | stage 8-9
      (Tazuke et al., 2002)
       larval stage | third instar
       primary spermatocyte cyst
      (Tazuke et al., 2002)
      Marker for
        Subcellular Localization
        CV Term
        hide External Data & Images
        Linkouts
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        •
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        •
        GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
        •
        BDGP expression data
        •
        Show all BDGP original images, annotations and embryonic microarray data for zpg
        FlyExpress - Embryonic expression images (BDGP data)
        •
        Show all FlyExpress processed images for zpg
        hide Alleles & Phenotypes
        hide Summary of Allele Phenotypes
        Lethality
        Allele
        viable
        lethal | recessive
        Sterility
        Allele
        female sterile (with Df(3L)CH12)
        male sterile
        male sterile (with Df(3L)ZN47)
        Phenotype manifest in
        Allele
        germ cell & germarium
        cystoblast
        cystocyte
        female germline stem cell
        nurse cell fusome
        testis (with Df(3L)ZN47)
        male germline stem cell (with Df(3L)ZN47)
        primary gonial cell (with Df(3L)ZN47)
        spermatogonium (with Df(3L)ZN47)
        hub cell (with Df(3L)ZN47)
        hub cell | supernumerary (with Df(3L)ZN47)
        cyst cell (with Df(3L)ZN47)
        ovary (with Df(3L)ZN47)
        egg chamber (with Df(3L)ZN47)
        female germline stem cell (with Df(3L)ZN47)
        ovary (with Df(3L)CH12)
        female germline stem cell (with Df(3L)CH12)
        female germline stem cell (with Df(3L)CH20)
        hide Classical Alleles ( 12 )
        For All Classical Alleles Show

        Allele of zpgClassMutagenStocksKnown lesion
        zpg1
          		0 --
          zpg20 --
          zpg3-ex0 Yes
          zpg30 Yes
          zpgLA006751 --
          zpgunspecified
            		0 --
            zpgz-09180 --
            zpgz-25331 --
            zpgz-25520 --
            zpgz-26790 --
            zpgz-38600 --
            zpgz-53521 --
            hide Alleles Carried on Transgenic Constructs ( 2 )
            For All Alleles Carried on Transgenic Constructs Show

            Allele of zpgClassMutagenStocksKnown lesion
            zpg+t6.150 Yes
            zpgGD23382 Yes
            hide Aneuploid Aberrations
            Useful deficiency
             
            Useful duplication
             
            Disrupted in
            (Tazuke et al., 2002)
            (Tazuke et al., 2002)
            (Tazuke et al., 2002)
            hide Transgenic Constructs & Insertions
            Transgenic Constructs
            Type of construct
            Name
            Expression data
            characterization construct
            P{zpg+t6.15}
            NA
            UAS construct
            P{GD2338}
            NA
            Insertions
            Type of insertions
            Name
            Expression data
            insertion of mobile activating element
            P{Mae-UAS.6.11}zpgLA00675
            NA
            insertion of enhancer trap
            P{lacW}zpg3
            No
            hide Related Comments
            Please look at the allele reports for the complete phenotype data
            Flies mutant for zpg are viable but sterile with very small gonads. In zpg mutant testes or ovaries, germline stem cells appear to divide but the early germ cells fail to differentiate, but rather, form small clusters and undergo apoptosis, as observed by staining with acridine orange.
            (Tazuke et al., 1998)
            Adult zpg mutant gonads contain small numbers of early germ cells, which resemble stem cells or early spermatogonia or oogonia, but lack later stages of germ cell differentiation.
            (Tazuke et al., 2002)
            Female germline stem cells in zpg mutants can divide, but the daughter cell normally destined to differentiate dies.
            (Gilboa et al., 2003)
            hide Gene Ontology: Function, Process & Cellular Component ( 4 )
            hide Molecular Function
            CV term
            Evidence
            References
            gap junction channel activity
            non-traceable author statement
            (Swiss-Prot Project Members, 2000.5.1)
            non-traceable author statement
            (Gilboa et al., 2001)
            non-traceable author statement
            (Tazuke et al., 2001)
            non-traceable author statement
            (Lin, 2002)
            hide Biological Process
            CV term
            Evidence
            References
            germ cell development
            inferred from mutant phenotype
            (Tazuke et al., 2002)
            traceable author statement
            (Lin, 2002)
            hide Cellular Component
            CV term
            Evidence
            References
            gap junction
            inferred from sequence or structural similarity with EMBL:AF115854; protein_id:AAD29306.1
            (FlyBase, 1992-)
            non-traceable author statement
            (Swiss-Prot Project Members, 2000.5.1)
            non-traceable author statement
            (Gilboa et al., 2001)
            non-traceable author statement
            (Tazuke et al., 2001)
            traceable author statement
            (Lin, 2002)
            integral to membrane
            non-traceable author statement
            (Swiss-Prot Project Members, 2000.5.1)
            hide Sequence Ontology: Class of Gene
            protein_coding_gene
             
            nuclear_gene
             
            hide Interactions & Pathways
            hide Summary of Genetic Interactions
            Interacts with
            Please look at the allele data for full details of the genetic interactions
            zpg allele
            Gene
            References
            zpgunspecified
            bgcn
            (Gilboa et al., 2003)
            dpp
            (Gilboa et al., 2003)
            bam
            (Gilboa et al., 2003)
            hide External Data
            Linkouts
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            hide Orthologs
            Genome-wide drosophilid orthologs
            Curated drosophilid orthologs
             
            Linkouts
            InParanoid orthologs - Eukaryotic orthologs
            •
            hide Functional Complementation between Species
            hide Inter-Species Misexpression Data
            Produces phenotype in
            Produces NO phenotype in
            hide Stocks & Reagents
            hide Stocks Listed in FlyBase ( 5 )
            VDRC
            v33277
            w1118; P{GD2338}v33277
            v33278
            w1118; P{GD2338}v33278
            Bloomington
            9918
            zpgz-2533 st1/TM3, Sb1
            9919
            zpgz-5352 bw1/TM6B, Tb1
            22247
            y1 w67c23; P{Mae-UAS.6.11}zpgLA00675 CG8368LA00675/TM3, Sb1 Ser1
            hide Genomic Clones ( 2 )
            hide cDNA Clones ( 9 )
            cDNA Clones, Fully Sequenced
            BDGP DGC clones
            Other clones
            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            hide RNAi & Array Information
            Affy Oligo
            Linkouts
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            hide Antibody Information
            polyclonal
            (Tazuke et al., 2002)
            hide Other Information
            hide Discoverer
            hide Etymology
            hide Identification
            hide Position Effect Variegation Data
            hide Relationship to Other Genes
            Source for database identity of
            Source for identity of: inx4 CG10125
            (Phelan and Starich, 2001)
            Source for database merge of
            Source for merge of: zpg inx4
            (Tazuke et al., 2002)
            Additional comments
            hide Comments About Role
            zpg is required for the survival of differentiating early germ cells during gametogenesis in both sexes.
            (Tazuke et al., 2002)
            hide Comments About Molecular Function
            hide Other Comments
            Expression is enriched in embryonic gonads.
            (Shigenobu et al., 2006)
            hide External Crossreferences & Linkouts
            Sequence Crossreferences
            RefSeq (Transcripts)
            •
            RefSeq (Proteins)
            •
            DDBJ / EMBL / GenBank
            •
            UniProtKB/Swiss-Prot
            •
            Other Crossreferences
            InterPro domains - A database of protein families, domains, and functional sites
            •
            Innexin (IPR000990)
            Linkouts
            BDGP expression data
            •
            Show all BDGP original images, annotations and embryonic microarray data for zpg
            BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions
            •
            DEDB - Drosophila exon database: splicing graphs
            •
            DRSC - RNAi screening (Harvard): high-throughput cell culture data and design
            •
            FLIGHT - Cell culture data for RNAi and other high-throughput technologies
            •
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            •
            FlyExpress - Embryonic expression images (BDGP data)
            •
            FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans
            •
            GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies
            •
            GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data
            •
            InParanoid orthologs - Eukaryotic orthologs
            •
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            •
            PANTHER - Protein classification by function, families, and pathways
            •
            hide Synonyms & Secondary IDs ( 6 )
            Reported As
            Symbol Synonym
            CG10125
            (Tazuke et al., 2002, )
            Dm-inx4
            (Phelan and Starich, 2001)
            inx-4
            (Chiusano et al., 2005)
            inx4
            (Stebbings et al., 2002, )
            zpg
            (Shigenobu et al., 2006, Shigenobu et al., 2006, Chau et al., 2008)
            Name Synonym
            zero population growth
            (Tazuke et al., 1998, Shigenobu et al., 2006)
            Secondary FlyBase IDs
            • FBgn0035706
            hide References ( 28 )
            Generate a list of
            List References by type
            hide Recent research papers ( 2 )
            Shigenobu et al., 2006, Proc. Natl. Acad. Sci. USA 103(37): 13728--13733
            Molecular characterization of embryonic gonads by gene expression profiling in Drosophila melanogaster. [FBrf0193273]
            Shigenobu et al., 2006, Dev., Growth Diffn 48(1): 49--57
            Isolation of germline cells from Drosophila embryos by flow cytometry. [FBrf0190265]
            Show all research papers ...
            hide Recent reviews (0)
            All reviews listed in FlyBase were published before 2006
            Show reviews ...