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Gene Dmel\wit

General Information
Symbol	Dmel\wit	Species	D. melanogaster
Name	wishful thinking	Annotation symbol	CG10776
Feature type	protein_coding_gene	FlyBase ID	FBgn0024179
Gene Model Status	Current	Stock availability	15 publicly available
Also Known As	l(3)64Aa, STK-D
Genomic Location
Chromosome (arm)	3L	Recombination map
Cytogenetic map	64A5-64A5	Sequence location	3L:4,064,152..4,071,717 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene wishful thinking is referred to in FlyBase by the symbol Dmel\wit (CG10776, FBgn0024179). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: transforming growth factor beta receptor activity, type II. There is experimental evidence that it is involved in the biological process: neuromuscular synaptic transmission; lateral inhibition; neuromuscular junction development. 39 alleles are reported. The phenotypes of these alleles are annotated with: wing vein L5; posterior crossvein; NMJ bouton; bouton; chaeta; neuromuscular junction; embryonic/larval neuromuscular junction; postsynaptic membrane; synapse; embryonic/larval muscle system. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: Protein kinase, ATP binding site; Protein kinase, catalytic domain; Protein kinase-like domain; TGF-beta receptor/activin receptor, type I/II. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed during early pupal stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1640409_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of wit. Gene sequence location is 3L:4064152..4071717.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 64A5-64A5   Limits computationally determined from genome sequence between P{PZ}l(3)rG166rG166 and P{PZ}sinu06524
Experimentally Determined Cytological Location
Cytogenetic map Notes References 64A1-64A5   (determined by in situ hybridisation)   (Szeged Stock Center, 1998-) 64A-64A     (Chavez et al., 1998) 64A1-64A5   (determined by in situ hybridisation)   (Deak et al., 1997)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\wit for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.46 Stop-codon suppression (UAG) postulated; FBrf0216884. gene_with_stop_codon_read_through ; SO:0000697 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) wit-RA FBtr0073279  NM_079953   4217   903 wit-RB FBtr0330072  NM_001274466   4217   913
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein wit-PA   FBpp0073135   100.3   903   6.99     NP_524692   AAF47832 wit-PB   FBpp0303105   101.4   913   6.82     NP_001261395   AGB94090
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) TGF-beta receptor/activin receptor, type I/II (IPR000472)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA698052   AA698177   AE014296 AAF47832   AF237561 AAF60175   AF420466 AAL16073   AE014296 AGB94090   AI135703   AW941769   AY119514 AAM50168   BG637674   BI173393   CO184867   CO266679   CO338933   CZ479052   CZ488074   DU708643   EC050988   EC054473   EC221967   EC253512   EC254240
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q9VZI9 Q95V83 Q9NGX8
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 11 -- 14   presumptive embryonic/larval central nervous system | restricted   (Aberle et al., 2002) ventral nerve cord primordium | restricted   (Aberle et al., 2002) embryonic stage 14   amnioserosa   (Aberle et al., 2002) embryonic stage 15 -- 17   presumptive embryonic/larval central nervous system   (Aberle et al., 2002) embryonic stage 16 -- 17   embryonic/larval midgut | restricted   (Aberle et al., 2002)
Additional Descriptive Data
	Embryonic expression first observed at stage 11, primarily in the CNS and in the labral sensory complex primordia of the PNS; expression in restricted clusters of cells. Expression in CNS expands to include more cells and continues to end of embryogenesis. In stage 14 embryos, also expressed is scattered cells in the dorsal closure furrow. In stage 16 embryos, also expressed in the 'furrows' (constrictions?) of the developing midgut. (Aberle et al., 2002)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 13   RP2 motor neuron   (Aberle et al., 2002) VUM neuron   (Aberle et al., 2002) embryonic stage 13 -- 14   ventral nerve cord primordium | restricted   (Aberle et al., 2002) embryonic stage 13 -- 17   lateral cord neuron   (Aberle et al., 2002) embryonic stage 16 -- 17   motor neuron   (Aberle et al., 2002) larval stage   embryonic/larval brain   (Aberle et al., 2002) first instar larval stage -- second instar larval stage   ventral nerve cord   (Aberle et al., 2002) third instar larval stage   imaginal disc   (Aberle et al., 2002) embryonic/larval neuromuscular junction   • terminal bouton (Higashi-Kovtun et al., 2010) embryonic/larval neuromuscular junction   • postsynaptic membrane (Higashi-Kovtun et al., 2010) ventral nerve cord   (Higashi-Kovtun et al., 2010)
Additional Descriptive Data
	Expressed specifically in the nervous system from embryonic stages until third instar larvae. In embryos from stage 13 high levels of expression in the CNS, in later embryos highest in motor neurons; weak expression in sensory neurons of the PNS. In larvae, expressed in brain from first through third instars; expression in ventral nerve cord ceases during third instar. Expression also observed in all imaginal discs. (Aberle et al., 2002)
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, adult salivary gland, larval trachea, adult spermathecae, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate   High  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    215.375 Larval Midgut    287.6 Larval Hindgut    69.4 Larval Malpighian Tubules    455.1 Larval Fat Body    145.5 Larval Salivary Gland    12.2 Larval Trachea    205.525 Larval Carcass    84.35 Adult Head    205.1 Adult Eye    374.5 Adult Brain    385.9 Adult Thoracic-Abdominal Ganglion    319 Adult Crop    212.2 Adult Midgut    224.5 Adult Hindgut    66.5 Adult Malpighian Tubules    348.4 Adult Fat Body    392.8 Adult Salivary Gland    279 Adult Heart    416.525 Adult VirginFemale Spermatheca    160.3 Adult InseminatedFemale Spermatheca    301.3 Adult Ovary    20.7 Adult Testis    28.7 Adult Male Accessory Gland    41 Adult Carcass    120 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    205.1   NA  Eye    374.5   NA  Brain    385.9   215.375  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    319   NA  Crop    212.2   287.6  Midgut    224.5   69.4  Hindgut    66.5   455.1  Malpighian Tubules    348.4   145.5  Fat Body    392.8   12.2  Salivary Gland    279   NA  Heart    416.525   205.525  Trachea    NA   NA  VirginFemale Spermatheca    160.3   NA  InseminatedFemale Spermatheca    301.3   NA  Ovary    20.7   NA  Testis    28.7   NA  Male Accessory Gland    41   84.35  Carcass    120 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    14 central nervous system, larvae L3    18 central nervous system, pupae P8    25 head, virgin 1-day female    14 head, virgin 4-day female    12 head, virgin 20-day female    10 head, mated 1-day female    13 head, mated 4-day female    13 head, mated 20-day female    10 head, mated 1-day male    19 head, mated 4-day male    20 head, mated 20-day male    18 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    1 digestive system, larvae L3 wandering    6 digestive system, 1-day adult    8 digestive system, 4-day adult    8 digestive system, 20-day adult    8 fat body, larvae L3 wandering    2 fat body, white prepupae    2 fat body, pupae P8    9 carcass, larvae L3 wandering    9 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    3 ovary, virgin 4-day female    1 ovary, mated 4-day female    0 testis, mated 4-day male    2 accessory gland, mated 4-day male    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of very low expression. Peak expression observed during early pupal stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    1 embryo 02-04hr    1 embryo 04-06hr    0 embryo 06-08hr    1 embryo 08-10hr    1 embryo 10-12hr    3 embryo 12-14hr    6 embryo 14-16hr    8 embryo 16-18hr    6 embryo 18-20hr    5 embryo 20-22hr    6 embryo 22-24hr    7 larva L1    6 larva L2    5 larva L3 12hr old    3 larva L3 puffstage 1-2    6 larva L3 puffstage 3-6    8 larva L3 puffstage 7-9    8 white prepupae new    9 white prepupae 12hr    13 white prepupae 24hr    21 pupae 2d postWPP    14 pupae 3d postWPP    7 pupae 4d postWPP    6 adult male 01day    7 adult male 05day    9 adult male 30day    8 adult female 01day    5 adult female 05day    3 adult female 30day    3 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    1 Schneider line 2 Sg4    0 embryonic 1182-4H    0 embryonic GM2    1 embryonic Kc167    1 embryonic S1    3 embryonic S3    1 leg disc CME L1    0 wing disc CME-W2    1 wing disc ML-DmD8    1 wing disc ML-DmD9    0 wing disc ML-DmD16-c3    1 wing disc ML-DmD21    0 wing disc ML-DmD32    0 haltere disc ML-DmD17-c3    0 eye-antennal disc ML-DmD11    0 antennal disc ML-DmD20-c5    0 mixed discs ML-DmD4-c1    0 CNS ML-DmBG1-c1    2 CNS ML-DmBG2-c2    11 tumorous blood cells mbn2    0 ovary fGS/OSS    2 ovary OSC    1 ovary OSS    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    4 cold shock, 4-day adult    4 heat shock, 4-day adult    4 Cadmium 50 mM 6 hrs, larvae L3    4 Cadmium 50 mM 12 hrs, larvae L3    5 Cadmium 50 mM 48 hrs, 4-day adult    4 Cadmium 100 mM 48 hrs, 4-day adult    3 Copper 0.5 mM 12 hrs, larvae L3    2 Copper 15 mM 48 hrs, 4-day adult    6 Zinc 5 mM 12 hrs, larvae L3    3 Zinc 4.5 mM 48 hrs, 4-day adult    9 Ethanol 2.5% 3 hrs, larvae L3    3 Ethanol 5% 3 hrs, larvae L3    9 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    4 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    3 Caffeine 25 mg/ml 48 hrs, 4-day adult    3 Paraquat 5 mM 48 hrs, 4-day adult    5 Paraquat 10 mM 48 hrs, 4-day adult    5 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    2 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010) mE2_34_mRNA_expression_cluster_18
External Data & Images
Linkouts	BDGP expression data • GH13548 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024179 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10776-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal (with Df(3L)GN50) witS126215 lethal (with witA12) witB11 lethal (with witB11) witA12 lethal | maternal effect | pharate adult stage witG15 lethal | pharate adult stage witunspecified lethal | pharate adult stage (with witA12) witC175 lethal | pharate adult stage (with witC175) witA12 lethal | pharate adult stage | recessive witunspecified lethal | recessive witA12 witB11 witS126215 viable witLA00504 viable, with Scer\GAL4pnr-MD237 witGD55 witGD14424 Sterility Allele fertile witLA00504 Other Phenotypes Allele neuroanatomy defective (with witA12) witB11 witHA3 neuroanatomy defective (with witB11) witA12 neuroanatomy defective (with witHA2) witHA5 neuroanatomy defective (with witHA3) witA12 neuroanatomy defective (with witHA4) witHA5 neuroanatomy defective (with witHA5) witHA2 witHA4 neuroanatomy defective | larval stage (with witA12) witB11 neuroanatomy defective | larval stage (with witB11) witA12 neuroanatomy defective | third instar larval stage (with witA12) witB11 neuroanatomy defective | third instar larval stage (with witB11) witA12 neurophysiology defective (with witA12) witB11 witC175 neurophysiology defective (with witB11) witA12 neurophysiology defective (with witC175) witA12 neurophysiology defective (with witHA4) witHA5 neurophysiology defective (with witHA5) witHA4 neurophysiology defective | larval stage (with witA12) witB11 neurophysiology defective | larval stage (with witB11) witA12 neurophysiology defective | third instar larval stage (with witA12) witHA3 neurophysiology defective | third instar larval stage (with witHA3) witA12 size defective | third instar larval stage (with witA12) witHA3 size defective | third instar larval stage (with witHA3) witA12 visible, with Scer\GAL4pnr-MD237 witGD14424 wild-type wit+tSE9 wit+tSE32 wit+tXB.SE32 withs.PM witUbi-p63E.PM witΔC.genomic wild-type, with Scer\GAL4G14 witScer\UAS.ΔC.T:Zzzz\FLAG Phenotype manifest in Allele bouton (with witA12) witB11 bouton (with witB11) witA12 bouton (with witHA2) witHA5 bouton (with witHA4) witHA5 bouton (with witHA5) witHA2 witHA4 chaeta, with Scer\GAL4pnr-MD237 witGD14424 embryonic/larval muscle system (with witHA2) witHA5 embryonic/larval muscle system (with witHA4) witHA5 embryonic/larval muscle system (with witHA5) witHA2 witHA4 embryonic/larval neuromuscular junction (with witA12) witB11 embryonic/larval neuromuscular junction (with witB11) witA12 embryonic/larval neuromuscular junction | third instar larval stage (with witA12) witB11 witHA3 embryonic/larval neuromuscular junction | third instar larval stage (with witB11) witA12 embryonic/larval neuromuscular junction | third instar larval stage (with witHA3) witA12 femur | pharate adult stage (with witA12) witC175 femur | pharate adult stage (with witC175) witA12 leg | pharate adult stage (with witA12) witC175 leg | pharate adult stage (with witC175) witA12 metathoracic femur | pharate adult stage (with witA12) witC175 metathoracic femur | pharate adult stage (with witC175) witA12 metathoracic tibia | pharate adult stage (with witA12) witC175 metathoracic tibia | pharate adult stage (with witC175) witA12 neuromuscular junction witS126215 neuromuscular junction (with witA12) witB11 neuromuscular junction (with witB11) witA12 neuromuscular junction (with witHA2) witHA5 neuromuscular junction (with witHA4) witHA5 neuromuscular junction (with witHA5) witHA2 witHA4 NMJ bouton (with witA12) witB11 NMJ bouton (with witB11) witA12 NMJ bouton | larval stage (with witA12) witB11 NMJ bouton | larval stage (with witB11) witA12 NMJ bouton | third instar larval stage (with witA12) witHA3 NMJ bouton | third instar larval stage (with witHA3) witA12 posterior crossvein, with Scer\GAL4A9 witΔI.Scer\UAS postsynaptic membrane (with witA12) witC175 postsynaptic membrane (with witC175) witA12 synapse, with Scer\GAL4Rapgap1-OK6 witScer\UAS.ΔC.T:Zzzz\FLAG synapse (with witA12) witB11 synapse (with witB11) witA12 synapse | larval stage witunspecified tarsal segment | pharate adult stage (with witA12) witC175 tarsal segment | pharate adult stage (with witC175) witA12 tibia | pharate adult stage (with witA12) witC175 tibia | pharate adult stage (with witC175) witA12 type I bouton | third instar larval stage (with witA12) witB11 type I bouton | third instar larval stage (with witB11) witA12 wing vein L5, with Scer\GAL4A9 witΔI.Scer\UAS
Classical Alleles ( 17 )
For All Classical Alleles Show
Allele of wit Class Mutagen Stocks Known lesion witA12 2 Yes witB11 2 -- witLA00504 P-element activity 2 -- witd02492 1 -- wite00566 1 -- wite01243 piggyBac transposase 1 -- witC175 0 Yes witf05487 piggyBac transposase 0 -- witG15 0 -- witG4 0 -- witHA1 hypomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes witHA2 loss of function allele ethyl methanesulfonate 0 -- witHA3 loss of function allele ethyl methanesulfonate 0 Yes witHA4 loss of function allele ethyl methanesulfonate 0 Yes witHA5 loss of function allele ethyl methanesulfonate 0 Yes witS126215 Delta2-3 P-element activity 0 Yes witunspecified 0 --
Alleles Carried on Transgenic Constructs ( 22 )
For All Alleles Carried on Transgenic Constructs Show
Allele of wit Class Mutagen Stocks Known lesion witGD14424 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes witGD55 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes witHMS02298 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes witJF01969 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes witKK100911 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes wit+tSE32 in vitro construct - genomic fragment 0 Yes wit+tSE9 in vitro construct - genomic fragment 0 Yes wit+tXB.SE32 in vitro construct - genomic fragment 0 Yes wit5'.Ubi-p63E in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis in vitro construct - deletion 0 Yes witdsRNA.cAa in vitro construct - RNAi 0 Yes witGD2551 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes withs.PM in vitro construct - regulatory fusion 0 Yes witNIG.10776R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes witScer\UAS.cMa in vitro construct - regulatory fusion 0 Yes witScer\UAS.T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes witScer\UAS.ΔC.T:Zzzz\FLAG in vitro construct - coding region fusion in vitro construct - deletion 0 Yes wittB.SE32 in vitro construct - genomic fragment 0 Yes wittHS in vitro construct - genomic fragment 0 Yes wittSE20 in vitro construct - genomic fragment 0 Yes witUbi-p63E.PM in vitro construct - regulatory fusion in vitro construct - site directed mutagenesis 0 Yes witΔC.genomic in vitro construct - site directed mutagenesis 0 Yes witΔI.Scer\UAS in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3L)A466 (Kulkarni et al., 1994) Df(3L)BSC369 (Christensen et al., 2007.10.29) Df(3L)C175 (Marques et al., 2002, Harrison et al., 1995) Df(3L)Exel6099 (Ryder, 2004.4.26) Df(3L)GN19 (Harrison et al., 1995) Df(3L)GN34 (Harrison et al., 1995) Df(3L)GN50 (Szeged Stock Center, 1998-, Deak et al., 1997) Df(3L)HR298 (Kulkarni et al., 1994, Harrison et al., 1995) Df(3L)TE1 (Kulkarni et al., 1994, Harrison et al., 1995)
Not disrupted in	Df(3L)9-7K (Kulkarni et al., 1994) Df(3L)TE3 (Kulkarni et al., 1994)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD55} NA P{GD2551} NA P{GD14424} NA P{KK100911} NA P{NIG.10776R} NA P{TRiP.HMS02298} NA P{TRiP.JF01969} NA P{UAS-wit.HA} NA P{UAS-wit.M} NA P{UAS-wit.ΔC.T:FLAG} NA P{UAS-witΔI} NA heat-shock construct P{Hsp70-wit.M} NA characterization construct P{B.SE32} NA P{dib+tHS} NA P{SE9} NA P{SE20} NA P{SE32} NA P{Ubi-p63E.sax-EC.wit-IC} NA P{Ubi-p63E.tkv-EC.wit-IC} NA P{Ubi-p63E.wit-EC.sax-IC} NA P{Ubi-p63E.wit-EC.tkv-IC} NA P{Ubi-p63E-wit.5'} NA P{Ubi-wit.M} NA P{wit.ΔC.genomic} NA P{XB.SE32} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}wite00566 NA PBac{RB}wite01243 NA PBac{WH}witf05487 NA insertion of mobile activating element P{Mae-UAS.6.11}witLA00504 NA P{XP}witd02492 NA insertion of enhancer trap P{lacW}witS126215 No
Gene Ontology: Function, Process & Cellular Component ( 12 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) neuromuscular junction development inferred from mutant phenotype (Marques et al., 2002, Aberle et al., 2002, Marques et al., 2003, James and Broihier, 2011) neuromuscular synaptic transmission inferred from mutant phenotype (Marques et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 9 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000719, InterPro:IPR017441 (FlyBase Curators et al., 2004-) protein kinase activity non-traceable author statement (Morrison et al., 2000) transforming growth factor beta-activated receptor activity inferred from electronic annotation with InterPro:IPR000472 (FlyBase Curators et al., 2004-) transforming growth factor beta receptor activity, type II inferred from sequence or structural similarity (Ruberte et al., 1995, Marques et al., 1996) non-traceable author statement (Chavez, 2000.2.20) traceable author statement (Chavez et al., 1998)
Biological Process
CV term Evidence References
dendrite morphogenesis non-traceable author statement (Grueber and Jan, 2004) protein phosphorylation inferred from electronic annotation with InterPro:IPR000719 (FlyBase Curators et al., 2004-) inferred from sequence or structural similarity (Ruberte et al., 1995) non-traceable author statement (Morrison et al., 2000) traceable author statement (Chavez et al., 1998) signal transduction inferred from sequence or structural similarity (Ruberte et al., 1995, Chavez et al., 1998)
Cellular Component
CV term Evidence References
membrane inferred from electronic annotation with InterPro:IPR000472 (FlyBase Curators et al., 2004-) plasma membrane inferred from sequence or structural similarity (Ruberte et al., 1995) traceable author statement (Chavez et al., 1998)
Sequence Ontology: Class of Gene
	gene_with_stop_codon_read_through (Jungreis et al., 2011) nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
wit - CG10418 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wit - CG15098 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wit - CG6701 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wit - SmD3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wit - SmG anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wit - Zn72D anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) wit - mRpL44 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions wit allele Gene References witA12 Fmrf (Marques et al., 2003) spin (Sweeney and Davis, 2002) witB11 Fmrf (Marques et al., 2003) spin (Sweeney and Davis, 2002) tkv::wit (McCabe et al., 2004) witHA3 Mad (McCabe et al., 2004) Med (McCabe et al., 2004) sax (McCabe et al., 2004) tkv (McCabe et al., 2004) tkv::wit (McCabe et al., 2004) witHA4 hiw (McCabe et al., 2004) witunspecified tkv::wit (McCabe et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 68828 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024179 DroID - A comprehensive database of gene and protein interactions. • FBgn0024179 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44096 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q95V83
Orthologs
OrthoDB Orthologs (62) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6GQQQC Dipteran inclusive ortholog search EOG6K3M48 Insect inclusive ortholog search EOG634V20 Arthropod inclusive ortholog search EOG6CJSZ1 Metazoa inclusive ortholog search EOG6TXQK5
Orthologs in Drosophila Species (EOG6GQQQC)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\wit     Drosophila simulans   Dsim\GD13298 Y   Drosophila sechellia   Dsec\GM14019 Y   Drosophila erecta   Dere\GG14225 Y   Drosophila yakuba   Dyak\GE20653 Y   Drosophila ananassae   Dana\GF24409 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10555 Y   Drosophila persimilis   Dper\GL16800 Y   Drosophila willistoni   Dwil\GK18023 Y   Drosophila virilis   Dvir\GJ13615 Y   Drosophila mojavensis   Dmoj\GI11419 Y   Drosophila grimshawi   Dgri\GH24979 Y
Orthologs in non-Drosophila Dipterans (EOG6K3M48)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL004165   Anopheles gambiae Malaria mosquito  Agam\AGAP005567   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ004526
Orthologs in non-Dipteran Insects (EOG634V20)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\wit   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG005861   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH13354   Atta cephalotes Leafcutter ant  Acep\ACEP26476   Camponotus floridanus Florida carpenter ant  Cflo\CFLO12631   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22254   Linepithema humile Argentine ant  Lhum\LH11245   Pogonomyrmex barbatus Red harvester ant  Pbar\PB21852   Solenopsis invicta Red fire ant  Sinv\SINV23298   Tribolium castaneum Red flour beetle  Tcas\wit
Orthologs in non-Insect Arthropods (EOG6CJSZ1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Ixodes scapularis Deer tick  Isca\ISCW020983
Orthologs in non-Arthropod Metazoa (EOG6TXQK5)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\daf-4 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_024092gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_017511gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_000814gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_011711gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012281gn Y Danio rerio Zebrafish  Drer\bmpr2a Y Danio rerio Zebrafish  Drer\bmpr2b Y Danio rerio Zebrafish  Drer\tgfbr2 Y Danio rerio Zebrafish  Drer\acvr2b Y Danio rerio Zebrafish  Drer\acvr2a Y Danio rerio Zebrafish  Drer\ENSDARG00000059363 Y Danio rerio Zebrafish  Drer\ENSDARG00000079944 Y Danio rerio Zebrafish  Drer\si:dkey-101k6.5 Y Danio rerio Zebrafish  Drer\ENSDARG00000041516 Y Xenopus tropicalis Western clawed frog  Xtro\tgfbr2 Y Xenopus tropicalis Western clawed frog  Xtro\bmpr2 Y Xenopus tropicalis Western clawed frog  Xtro\acvr2b Y Xenopus tropicalis Western clawed frog  Xtro\acvr2a Y Gallus gallus Domestic chicken  Ggal\LOC424261 Y Gallus gallus Domestic chicken  Ggal\ACVR2A Y Gallus gallus Domestic chicken  Ggal\TGFBR2 Y Gallus gallus Domestic chicken  Ggal\ACVR2B Y Gallus gallus Domestic chicken  Ggal\BRK-3 Y Mus musculus House mouse  Mmus\Bmpr2 Y Mus musculus House mouse  Mmus\Tgfbr2 Y Mus musculus House mouse  Mmus\Acvr2a Y Mus musculus House mouse  Mmus\ENSMUSG00000061393 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000022196 Y Rattus norvegicus Norway rat  Rnor\Acvr2a Y Rattus norvegicus Norway rat  Rnor\Acvr2b Y Rattus norvegicus Norway rat  Rnor\Tgfbr2 Y Homo sapiens Human  Hsap\TGFBR2 Y Homo sapiens Human  Hsap\ACVR2A Y Homo sapiens Human  Hsap\BMPR2 Y Homo sapiens Human  Hsap\ACVR2B Y
Human Orthologs (4)
Gene OMIM HGNC Hsap\TGFBR2     Hsap\ACVR2A     Hsap\BMPR2     Hsap\ACVR2B
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD13298 Drosophila sechellia Dsec\GM14019 Drosophila erecta Dere\GG14225 Drosophila yakuba Dyak\GE20653 Drosophila ananassae Dana\GF24409 Drosophila pseudoobscura pseudoobscura Dpse\GA10555 Drosophila persimilis Dper\GL16800 Drosophila willistoni Dwil\GK18023 Drosophila virilis Dvir\GJ13615 Drosophila mojavensis Dmoj\GI11419 Drosophila grimshawi Dgri\GH24979
Stocks & Reagents
Stocks Listed in FlyBase ( 15 )
Bloomington	25949 y1 v1; P{TRiP.JF01969}attP2 41906 y1 sc* v1; P{TRiP.HMS02298}attP2
Harvard	- P{XP}witd02492 - PBac{RB}wite00566 - PBac{RB}wite01243
Kyoto	120504 y* w*; P{Mae-UAS.6.11}witLA00504 108085 bw1; witA12 st1/TM6B, Tb1 108086 bw1; witB11 st1/TM6B, Tb1
VDRC	v103808 P{KK100911}VIE-260B v865 w1118; P{GD55}v865 v42244 w1118; P{GD14424}v42244 v42245 w1118; P{GD14424}v42245/TM3
	More stocks available...
Genomic Clones ( 1 )
	BACR09C02 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 13 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH13548
Other clones	DPiM_GH13548
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	HL03685 RE16303
Other clones	36434 214245.22 214447.25 EC28710 EC050988 EC054473 EK147221 EP00351 HL03890
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024179 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44096
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: wit CG10776 (FlyBase, 1996-) Source for merge of: wit l(3)64Aa (Chavez et al., 2000) Source for merge of: wit l(3)S126215 (Aberle et al., 2002) Source for merge of: wit SE20 (O'Connor, 2000.12.5) Source for merge of: wit Stk-D (Basler, 2000.11.30)
Additional comments	Source for merge of wit CG10776 was sequence comparison (date:000401). (FlyBase, 1996-)
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) wit regulates expression of Fmrf. wit may globally regulate neuromuscular junction (NMJ) function by controlling both the growth and transmitter release properties of the synapse as well as the expression of systemic modulators of NMJ synaptic activity. (Marques et al., 2003) wit appears to function as a presynaptic receptor that regulates synaptic size at the neuromuscular junction. (Aberle et al., 2002) wit has a role in neuromuscular junction synapse assembly and activity. (Marques et al., 2002) A new Type II receptor, a candidate receptor for dpp, scw and gbb. (Marques et al., 1996) Discussed during genetic analysis of the Ras64B-Rop region. (Harrison et al., 1995)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079953 NM_001274466 RefSeq (Proteins) • NP_524692 NP_001261395 DDBJ / EMBL / GenBank • AA698052 AA698177 AAF47832 AAF60175 AAL16073 AAM50168 AF237561 AF420466 AGB94090 AI135703 AW941769 AY119514 BG637674 BI173393 CZ479052 CZ488074 DU708643 Entrez Gene - A searchable database of RefSeq genes. • 44096 UniProtKB/TrEMBL • Q9NGX8 Q9VZI9 Q95V83
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Protein kinase, catalytic domain (IPR000719) Protein kinase-like domain (IPR011009) Protein kinase, ATP binding site (IPR017441) TGF-beta receptor/activin receptor, type I/II (IPR000472) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • GH13548
Linkouts
	BioGRID - A database of protein and genetic interactions • 68828 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024179 DroID - A comprehensive database of gene and protein interactions. • FBgn0024179 DRSC - Results from RNAi screens. • FBgn0024179 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024179 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10776-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024179 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44096 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /torstoll/wishflt1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44096 modMine - Data generated by the modENCODE project. • FBgn0024179 SignaLink - A protein-protein and protein-miRNA interaction database with multi-layered structure and multiple confidence scores. • Q95V83
Synonyms & Secondary IDs ( 16 )
Reported As
Symbol Synonym	1262/15 (Deak et al., 1997) ALK3/BMPRII (Morrison et al., 2000) CG10776 (Marques et al., 2002, Morrison et al., 2000, Perkins et al., 1.15.2009, Keleman et al., 2009.8.5, Ni et al., 2010.12.1) l(3)64Aa (Featherstone et al., 2000, Chavez et al., 2000, Harrison, 1999.5.12, Kulkarni et al., 1994, ) l(3)S126215   l(3)SH12 (Harrison et al., 1995) SE20 (Marques et al., 1996, ) STK-D (Basler, 2000.11.30, Raftery and Sutherland, 1999, Ruberte et al., 1995) Stk-D   wit (Collins et al., 2006, Sun et al., 2012, Knox et al., 2007, Eaton and Davis, 2005, Ball et al., 2010, Christensen et al., 2007.10.29, Serpe and O'Connor, 2006, Herrero et al., 2007, Yuva-Aydemir et al., 2011, Chang et al., 2008, van der Plas et al., 2006, Wang et al., 2007, Marques, 2005, Boylan et al., 2008, Nahm et al., 2010, Jungreis et al., 2011, Chang et al., 2008, Ng, 2008, Goold and Davis, 2007, Vogler and Urban, 2008, Miguel-Aliaga et al., 2008, Kim and Marqués, 2012, Nakayama et al., 2006, Fradkin et al., 2008, Rodal et al., 2008, Korolchuk et al., 2007, Eade and Allan, 2009, Cordero et al., 2007, Koh et al., 2004, Haussmann et al., 2008, Davidson et al., 2009, Higashi-Kovtun et al., 2010, Nahm et al., 2010, Mosca and Schwarz, 2010, Xiong et al., 2010, Pilgram et al., 2011, O'Connor-Giles et al., 2008, Kim and Marqués, 2010, Rodal et al., 2011, Khuong et al., 2010, James and Broihier, 2011, Miller et al., 2012, Le and Wharton, 2012, Japanese National Institute of Genetics, 2012.5.21, Pennetier et al., 2012) Wit (Baumgardt et al., 2007, Yakoby et al., 2007, Dudu, 2006, Jensen et al., 2009) WIT (Gesualdi and Haerry, 2007)
Name Synonym	Serine/threonine kinase-D   wishful thinking (Chavez et al., 1998, Collins et al., 2006, Knox et al., 2007, Eaton and Davis, 2005, Ball et al., 2010, Marques, 2005, Chang et al., 2008, Korolchuk et al., 2007, Koh et al., 2004, Haussmann et al., 2008, Mosca and Schwarz, 2010, Miller et al., 2012) Wishful thinking (Herrero et al., 2007, Wang et al., 2007, Jensen et al., 2009, Higashi-Kovtun et al., 2010, Kim and Marqués, 2010, James and Broihier, 2011) Wishful Thinking (Wang et al., 2007)
Secondary FlyBase IDs
	FBgn0014282 FBgn0015807 FBgn0027362 FBgn0027993 FBgn0035493
References ( 122 )
Generate a list of	All references relating to this gene ( 122 )
List References by type	Research paper  ( 66 ) Supplementary material  ( 9 ) Review  ( 12 ) Personal communication to FlyBase  ( 15 ) Abstract  ( 11 ) FlyBase analysis  ( 3 ) Stock list  ( 2 ) DNA/RNA sequence record  ( 1 ) Curated genome annotation  ( 2 ) Conference report  ( 1 )
Recent research papers ( 11 )
	Gorostiza and Ceriani, 2013, J. Neurosci. 33(2): 687--696 Retrograde bone morphogenetic protein signaling shapes a key circadian pacemaker circuit. [FBrf0220479] Marmion et al., 2013, Dev. Biol. 375(1): 45--53 The Drosophila BMPRII, wishful thinking, is required for eggshell patterning. [FBrf0220785] Kim and Marqués, 2012, Dev. Neurobiol. 72(12): 1541--1558 The Ly6 neurotoxin-like molecule target of wit regulates spontaneous neurotransmitter release at the developing neuromuscular junction in Drosophila. [FBrf0219871] Le and Wharton, 2012, Dev. Dyn. 241(1): 200--214 Hyperactive BMP signaling induced by ALK2(R206H) requires type II receptor function in a Drosophila model for classic fibrodysplasia ossificans progressiva. [FBrf0217004] Miller et al., 2012, J. Neurosci. 32(40): 13776--13786 Analysis of synaptic growth and function in Drosophila with an extended larval stage. [FBrf0219554] Pennetier et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(9): 3389--3394 Size control of the Drosophila hematopoietic niche by bone morphogenetic protein signaling reveals parallels with mammals. [FBrf0217568] Sun et al., 2012, PLoS Genet. 8(2): e1002515 Neurophysiological Defects and Neuronal Gene Deregulation in Drosophila mir-124 Mutants. [FBrf0217508] James and Broihier, 2011, Development 138(15): 3273--3286 Crimpy inhibits the BMP homolog Gbb in motoneurons to enable proper growth control at the Drosophila neuromuscular junction. [FBrf0214390] Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Rodal et al., 2011, J. Cell Biol. 193(1): 201--217 A presynaptic endosomal trafficking pathway controls synaptic growth signaling. [FBrf0213353] Yuva-Aydemir et al., 2011, J. Neurosci. 31(19): 7005--7015 Spinster Controls Dpp Signaling during Glial Migration in the Drosophila Eye. [FBrf0213705] Show all research papers ...
Recent reviews ( 1 )
	Broadie et al., 2011, Dev. Neurobiol. 71(11): 1102--1130 Extracellular matrix and its receptors in drosophila neural development. [FBrf0216377] Show all reviews ...