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Gene: Dmel\unc-4

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General Information
Symbol
Dmel\unc-4
Species
D. melanogaster
Name
unc-4
Annotation Symbol
CG6269
Feature Type
protein_coding_gene
FlyBase ID
FBgn0024184
Gene Model Status
Current
Stock Availability
5 publicly available
Gene Snapshot
Unc4 is a homeodomain transcription factor expressed in distinct subsets of neurons in the embryonic and post-embryonic CNS. It regulates neuronal development and differentiation at least in part via repressing the expression of HGTX. Unc4 roles include neuronal specification and differentiation, specifically axonal pathfinding and axongenesis. [Date last reviewed: 2016-08-25]
Other Summaries
Also Known As
Dunc-4
Key Links
Genomic Location
Cytogenetic map
16C10-16D1
Sequence location
X:17,768,569..17,778,708 [+]
Recombination map
1-58
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI Genome Data Viewer
UCSC Genome Browser
Ensembl Genome Browser
PopFly Genome Browser
GO Summary Ribbons
Families, Domains and Molecular Function
Gene Group Membership (FlyBase)
Protein Family (UniProt, Sequence Similarities)
Belongs to the paired homeobox family. Unc-4 subfamily. (O77215)
Protein Domains/Motifs
InterPro
Homeobox domain; Homeobox-like domain superfamily; Homeobox, conserved site
Molecular Function (see GO section for details)
Experimental Evidence
-
Predictions / Assertions
sequence-specific DNA binding
Summaries
Gene Group Membership
PAIRED-LIKE HOMEOBOX TRANSCRIPTION FACTORS -
Paired-like (PRD-like) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. PRD-like transcription factors lack a paired domain but possess a homeodomain characteristic of the paired homeobox transcription factors. Some members also possess an Engrailed Homology 1 (EH1) and/or an OAR motif near the C-terminus. (Adapted from FBrf0232555 and FBrf0106517).
UniProt Contributed Function Data
Transcription factor that regulates synaptic specificity.
(UniProt, O77215)
Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\unc-4 or the JBrowse view of Dmel\unc-4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model
Gene model reviewed during 5.51
Gene model reviewed during 5.55
Alternative translation stop created by use of multiphasic reading frames within coding region.
Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
unc-4-RB
FBtr0340626
NM_001298460
2866
588
unc-4-RC
FBtr0340627
NM_133014
2995
588
unc-4-RD
FBtr0340628
NM_001298461
2870
300
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
unc-4-PB
FBpp0309490
61.1
588
8.41
NP_001285389
AHN59859
unc-4-PC
FBpp0309491
61.1
588
8.41
NP_573242
AAF48762
unc-4-PD
FBpp0309492
31.0
300
7.25
NP_001285390
AHN59860
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

588 aa isoforms: unc-4-PB, unc-4-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unc-4 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (2 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
sequence-specific DNA binding
inferred from electronic annotation with InterPro:IPR017970
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
regulation of transcription, DNA-templated
inferred from electronic annotation with InterPro:IPR017970
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Expression Data
Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\unc-4 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs and Phenotypes
Classical and Insertion Alleles ( 0 )
For All Classical and Insertion Alleles Show
 
Allele of unc-4
Class
Mutagen
Associated Insertion
Stocks
Known lesion
Other relevant insertions
Transgenic Constructs ( 5 )
Deletions and Duplications ( 0 )
Summary of Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (6)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\UNCX
6 of 15
Yes
Yes
Hsap\ALX1
1 of 15
No
No
Hsap\ALX3
1 of 15
No
No
Hsap\ALX4
1 of 15
No
No
Hsap\PRRX1
1 of 15
No
No
Hsap\PRRX2
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (5)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Uncx
6 of 15
Yes
Yes
Mmus\Alx1
1 of 15
No
No
Mmus\Alx4
1 of 15
No
No
Mmus\Prrx1
1 of 15
No
No
Mmus\Prrx2
1 of 15
No
No
Rattus norvegicus (Norway rat) (5)
Rnor\Uncx
6 of 13
Yes
Yes
Rnor\Alx1
1 of 13
No
No
Rnor\Alx4
1 of 13
No
No
Rnor\Prrx1
1 of 13
No
No
Rnor\Prrx2
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (2)
Xtro\uncx
2 of 12
Yes
Yes
Xtro\alx1
1 of 12
No
Yes
Danio rerio (Zebrafish) (7)
Drer\uncx
6 of 15
Yes
Yes
Drer\uncx4.1
5 of 15
No
Yes
Drer\alx3
2 of 15
No
Yes
Drer\alx4a
2 of 15
No
Yes
Drer\alx1
1 of 15
No
No
Drer\prrx1a
1 of 15
No
No
Drer\prrx1b
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\unc-4
3 of 15
Yes
Yes
Cele\dsc-1
2 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\HB-2
1 of 9
Yes
Yes
Saccharomyces cerevisiae (Brewer's yeast) (0)
No orthologs reported.
Schizosaccharomyces pombe (Fission yeast) (0)
No orthologs reported.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190CO9 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\unc-4
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005094
Drosophila simulans
Dsim\GD26823
Drosophila sechellia
Dsec\GM13524
Drosophila erecta
Dere\GG19131
Drosophila yakuba
Dyak\GE17683
Drosophila ananassae
Dana\GF19454
Drosophila pseudoobscura pseudoobscura
Dpse\GA19478
Drosophila persimilis
Dper\GL14568
Drosophila willistoni
Dwil\GK25621
Drosophila virilis
Dvir\GJ16592
Drosophila mojavensis
Dmoj\GI14686
Drosophila grimshawi
Dgri\GH24785
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EZU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013237
Glossina morsitans
Tsetse fly
Gmor\GMOY006492
Glossina morsitans
Tsetse fly
Gmor\GMOY008202
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_14517
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_14508
Mayetiola destructor
Hessian fly
Mdes\Mdes006342
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007285
Anopheles darlingi
American malaria mosquito
Adar\ADAC008055
Anopheles gambiae
Malaria mosquito
Agam\AGAP001495
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ017215
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0N47 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA005619
Danaus plexippus
Monarch butterfly
Dple\DPOGS200324-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL011564
Apis florea
Little honeybee
Aflo\4108345508
Apis mellifera
Western honey bee
Amel\LOC408443
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP12782
Bombus terrestris
Buff-tailed bumblebee
Bter\15209135
Linepithema humile
Argentine ant
Lhum\LH19475
Linepithema humile
Argentine ant
Lhum\LH19474
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001dd7
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000927
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12706
Tribolium castaneum
Red flour beetle
Tcas\TC005661
Pediculus humanus
Human body louse
Phum\PHUM093500
Pediculus humanus
Human body louse
Phum\PHUM093490
Rhodnius prolixus
Kissing bug
Rpro\RPRC011714
Rhodnius prolixus
Kissing bug
Rpro\RPRC011712
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI000498
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14529
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_13097
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0ND8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011175
Strigamia maritima
European centipede
Smar\SMAR011174
Ixodes scapularis
Black-legged tick
Isca\ISCW016210
Ixodes scapularis
Black-legged tick
Isca\ISCW016208
Stegodyphus mimosarum
African social velvet spider
Smim\KFM71523.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g00320
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_41198
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0B1L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Alx1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Prx1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Unc4-1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Alx4
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Alx1_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Unc4.1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000009645
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000018015
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000027213
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000025875
Gallus gallus
Domestic chicken
Ggal\UNCX
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000003324
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000025867
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000028236
Human Disease Model Data
FlyBase Human Disease Model Reports
    Alleles Reported to Model Human Disease (Disease Ontology)
    Download
    Models ( 0 )
    Allele
    Disease
    Evidence
    References
    Interactions ( 0 )
    Allele
    Disease
    Interaction
    References
    Comments ( 0 )
     
    Human Orthologs (via DIOPT v7.1)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    Complementation?
    Transgene?
    UNCX; UNC homeobox
    6 of 15
      Functional Complementation Data
      Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
      Interactions
      Summary of Physical Interactions
      esyN Network Diagram
      Interactions Browser
      Summary of Genetic Interactions
      esyN Network Diagram
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      Starting gene(s)
      Interaction type
      Interacting gene(s)
      Reference
      External Data
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      DroID - A comprehensive database of gene and protein interactions.
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      Pathways
      Gene Group - Pathway Membership (FlyBase)
      External Data
      Linkouts
      Genomic Location and Detailed Mapping Data
      Chromosome (arm)
      X
      Recombination map
      1-58
      Cytogenetic map
      16C10-16D1
      Sequence location
      X:17,768,569..17,778,708 [+]
      FlyBase Computed Cytological Location
      Cytogenetic map
      Evidence for location
      16C10-16D1
      Limits computationally determined from genome sequence between P{EP}EP1464 and P{EP}EP970
      Experimentally Determined Cytological Location
      Cytogenetic map
      Notes
      References
      16D1-16D2
      (Tabuchi et al., 1998)
      16D1-16D2
      (determined by in situ hybridisation)
      (Okano and Tabuchi, 1998.3.11)
      Experimentally Determined Recombination Data
      Location
      1-58
      (FlyBase, 2016)
      1-58.4
      (Comeron et al., 2012)
      Left of (cM)
      Right of (cM)
      Notes
      Stocks and Reagents
      Stocks (5)
      Genomic Clones (15)
       

      Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

      cDNA Clones (11)
       

      Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

      cDNA clones, fully sequences
      BDGP DGC clones
      Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      polyclonal
      (Lacin et al., 2014)
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of
      Source for database merge of
      Additional comments
      Other Comments
      A novel paired-like homeobox containing gene isolated by RT-PCR from Drosophila embryonic mRNA. Temporal and spatial expression patterns are characterised.
      (Tabuchi et al., 1998)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 35 )
      Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
      InterPro - A database of protein families, domains and functional sites
      Linkouts
      BioGRID - A database of protein and genetic interactions.
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      FlyMine - An integrated database for Drosophila genomics
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Synonyms and Secondary IDs (7)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
      Title
      References (40)