FB2021_02, released April 13, 2021

Gene: Dmel\unc-4

Open Close
General Information
Symbol
Dmel\unc-4
Species
D. melanogaster
Name
unc-4
Annotation Symbol
CG6269
Feature Type
protein_coding_gene
FlyBase ID
FBgn0024184
Gene Model Status
Current
Stock Availability
7 publicly available
Gene Summary
unc-4 (unc-4) encodes a homeodomain transcription factor expressed in distinct subsets of neurons in the embryonic and post-embryonic CNS. It regulates neuronal development and differentiation at least in part via repressing the expression of HGTX. It contributes to neuronal specification and differentiation, specifically axonal pathfinding and axongenesis. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Dunc-4

Key Links
Genomic Location
Cytogenetic map
16C10-16D1
Sequence location
X:17,768,569..17,778,708 [+]
Recombination map
1-58
RefSeq locus
NC_004354 REGION:17768569..17778708
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (4 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables DNA-binding transcription factor activity, RNA polymerase II-specific
inferred from electronic annotation with InterPro:IPR017970
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
enables sequence-specific double-stranded DNA binding
inferred from biological aspect of ancestor with PANTHER:PTN001551498
(assigned by GO_Central )
(Gaudet et al., 2011)
Biological Process (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
involved_in regulation of gene expression
inferred from biological aspect of ancestor with PANTHER:PTN001551498
(assigned by GO_Central )
(Gaudet et al., 2011)
involved_in regulation of transcription, DNA-templated
inferred from electronic annotation with InterPro:IPR017970
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Cellular Component (0 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (0 terms)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the paired homeobox family. Unc-4 subfamily. (O77215)
Protein Signatures (InterPro)
Summaries
Gene Snapshot
unc-4 (unc-4) encodes a homeodomain transcription factor expressed in distinct subsets of neurons in the embryonic and post-embryonic CNS. It regulates neuronal development and differentiation at least in part via repressing the expression of HGTX. It contributes to neuronal specification and differentiation, specifically axonal pathfinding and axongenesis. [Date last reviewed: 2019-03-21]
Gene Group (FlyBase)
PAIRED-LIKE HOMEOBOX TRANSCRIPTION FACTORS -
Paired-like (PRD-like) homeobox transcription factors are sequence-specific DNA binding proteins that regulate transcription. PRD-like transcription factors lack a paired domain but possess a homeodomain characteristic of the paired homeobox transcription factors. Some members also possess an Engrailed Homology 1 (EH1) and/or an OAR motif near the C-terminus. (Adapted from FBrf0232555 and FBrf0106517).
Protein Function (UniProtKB)
Transcription factor that regulates synaptic specificity.
(UniProt, O77215)
Summary (Interactive Fly)

homeodomain transcription factor - functions during post-embryonic development of the adult CNS to promote cholinergic neurotransmitter identity and suppress the GABA fate in one larval neuroblast lineage - promotes proper neuronal projections to the leg neuropil and a specific flight-related take-off behavior in a second larval lineage - acts peripherally to promote proprioceptive sensory organ development and the execution of specific leg-related behaviors

Gene Model and Products
Number of Transcripts
3
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\unc-4 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.51

Gene model reviewed during 5.55

Alternative translation stop created by use of multiphasic reading frames within coding region.

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
unc-4-RB
FBtr0340626
NM_001298460
2866
588
unc-4-RC
FBtr0340627
NM_133014
2995
588
unc-4-RD
FBtr0340628
NM_001298461
2870
300
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
unc-4-PB
FBpp0309490
61.1
588
8.41
NP_001285389
AHN59859
unc-4-PC
FBpp0309491
61.1
588
8.41
NP_573242
AAF48762
unc-4-PD
FBpp0309492
31.0
300
7.25
NP_001285390
AHN59860
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

588 aa isoforms: unc-4-PB, unc-4-PC
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\unc-4 using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\unc-4 in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 5 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of unc-4
Transgenic constructs containing regulatory region of unc-4
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 0 )
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\UNCX
6 of 15
Yes
Yes
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Uncx
6 of 15
Yes
No
Rattus norvegicus (Norway rat) (1)
Rnor\Uncx
7 of 13
Yes
Yes
Xenopus tropicalis (Western clawed frog) (1)
Xtro\uncx
4 of 12
Yes
Yes
Danio rerio (Zebrafish) (4)
Drer\uncx4.1
6 of 15
Yes
Yes
Drer\uncx
6 of 15
Yes
No
Drer\alx3
1 of 15
No
No
Drer\alx4a
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\unc-4
3 of 15
Yes
No
Cele\dsc-1
1 of 15
No
Yes
Arabidopsis thaliana (thale-cress) (1)
Atha\HB-2
1 of 9
Yes
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
No records found.
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG09190CO9 )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00005094
Drosophila simulans
Dsim\GD26823
Drosophila sechellia
Dsec\GM13524
Drosophila erecta
Dere\GG19131
Drosophila yakuba
Dyak\GE17683
Drosophila ananassae
Dana\GF19454
Drosophila pseudoobscura pseudoobscura
Dpse\GA19478
Drosophila persimilis
Dper\GL14568
Drosophila willistoni
Dwil\GK25621
Drosophila virilis
Dvir\GJ16592
Drosophila mojavensis
Dmoj\GI14686
Drosophila grimshawi
Dgri\GH24785
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150EZU )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17013237
Glossina morsitans
Tsetse fly
Gmor\GMOY006492
Glossina morsitans
Tsetse fly
Gmor\GMOY008202
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_14517
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_14508
Mayetiola destructor
Hessian fly
Mdes\Mdes006342
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL007285
Anopheles darlingi
American malaria mosquito
Adar\ADAC008055
Anopheles gambiae
Malaria mosquito
Agam\AGAP001495
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ017215
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0N47 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA005619
Danaus plexippus
Monarch butterfly
Dple\DPOGS200324-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL011564
Apis florea
Little honeybee
Aflo\4108345508
Apis mellifera
Western honey bee
Amel\LOC408443
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP12782
Bombus terrestris
Buff-tailed bumblebee
Bter\15209135
Linepithema humile
Argentine ant
Lhum\LH19475
Linepithema humile
Argentine ant
Lhum\LH19474
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:001dd7
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG000927
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12706
Tribolium castaneum
Red flour beetle
Tcas\TC005661
Pediculus humanus
Human body louse
Phum\PHUM093500
Pediculus humanus
Human body louse
Phum\PHUM093490
Rhodnius prolixus
Kissing bug
Rpro\RPRC011714
Rhodnius prolixus
Kissing bug
Rpro\RPRC011712
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI000498
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_14529
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_13097
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0ND8 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR011175
Strigamia maritima
European centipede
Smar\SMAR011174
Ixodes scapularis
Black-legged tick
Isca\ISCW016210
Ixodes scapularis
Black-legged tick
Isca\ISCW016208
Stegodyphus mimosarum
African social velvet spider
Smim\KFM71523.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur04g00320
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_41198
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0B1L )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Alx1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Prx1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Unc4-1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Alx4
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Alx1_1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Unc4.1
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000009645
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000018015
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000027213
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000025875
Gallus gallus
Domestic chicken
Ggal\UNCX
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000003324
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000025867
Gallus gallus
Domestic chicken
Ggal\ENSGALG00000028236
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (23)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
OdsH
3 of 10
al
1 of 10
CG9876
1 of 10
CG11294
1 of 10
CG32532
1 of 10
eve
1 of 10
ey
1 of 10
eyg
1 of 10
gsb
1 of 10
gsb-n
1 of 10
Gsc
1 of 10
oc
1 of 10
otp
1 of 10
Poxm
1 of 10
Poxn
1 of 10
Pph13
1 of 10
prd
1 of 10
Ptx1
1 of 10
repo
1 of 10
sv
1 of 10
toe
1 of 10
Vsx1
1 of 10
Vsx2
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Model Summary Ribbon
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    UNCX; UNC homeobox
    6 of 15
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        esyN Network Diagram
        Show neighbor-neighbor interactions:
        Select Layout:
        Legend:
        Protein
        RNA
        Selected Interactor(s)
        Interactions Browser
        View in Interactions Browser
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        unc-4 - GATAe
        two hybrid array
        (Shokri et al., 2019)
        unc-4 - Hr78
        two hybrid array
        (Shokri et al., 2019)
        unc-4 - Ubx
        bimolecular fluorescence complementation, fluorescence microscopy
        (Bischof et al., 2018)
        unc-4 - abd-A
        bimolecular fluorescence complementation, fluorescence microscopy
        (Bischof et al., 2018)
        Summary of Genetic Interactions
        esyN Network Diagram
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        External Data
        Linkouts
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        X
        Recombination map
        1-58
        Cytogenetic map
        16C10-16D1
        Sequence location
        X:17,768,569..17,778,708 [+]
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        16C10-16D1
        Limits computationally determined from genome sequence between P{EP}EP1464 and P{EP}EP970
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        16D1-16D2
        (Tabuchi et al., 1998)
        16D1-16D2
        (determined by in situ hybridisation)
        (Okano and Tabuchi, 1998.3.11)
        Experimentally Determined Recombination Data
        Location

        1-58

        (FlyBase, 2016)

        1-58.4

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (7)
        Genomic Clones (15)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (11)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        Antibody Information
        Laboratory Generated Antibodies
         

        polyclonal

        (Lacin et al., 2014)
        Commercially Available Antibodies
         
        Other Information
        Relationship to Other Genes
        Source for database identify of
        Source for database merge of
        Additional comments
        Other Comments

        A novel paired-like homeobox containing gene isolated by RT-PCR from Drosophila embryonic mRNA. Temporal and spatial expression patterns are characterised.

        (Tabuchi et al., 1998)
        Origin and Etymology
        Discoverer
        Etymology
        Identification
        External Crossreferences and Linkouts ( 37 )
        Sequence Crossreferences
        NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
        GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
        GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
        RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
        UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
        UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
        Other crossreferences
        BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
        Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
        Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
        Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
        Flygut - An atlas of the Drosophila adult midgut
        GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
        iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
        InterPro - A database of protein families, domains and functional sites
        KEGG Genes - Molecular building blocks of life in the genomic space.
        modMine - A data warehouse for the modENCODE project
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        DRSC - Results frm RNAi screens
        FLIGHT - Cell culture data for RNAi and other high-throughput technologies
        FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
        FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
        FlyMine - An integrated database for Drosophila genomics
        Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
        InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
        Synonyms and Secondary IDs (7)
        Datasets (0)
        Study focus (0)
        Experimental Role
        Project
        Project Type
        Title
        References (46)