FlyBase Gene Report: Dmel\robl

General Information

Symbol

Dmel\robl

Species

D. melanogaster

Name

roadblock

Annotation Symbol

CG10751

Feature Type

protein_coding_gene

FlyBase ID

FBgn0024196

Gene Model Status

Current

Stock Availability

8 publicly available

Gene Summary

roadblock (robl) encodes a dynein-associated protein involved in intracellular transport mediated by Dynein. It contributes to dendrite morphogenesis and mushroom body development. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
Interactive Fly

Also Known As

DLC, dynein light chain, l(2)k10408, LC7

Key Links

Genomic Location

Cytogenetic map

54B16-54B16

Sequence location

2R:17,456,472..17,457,129 [+]

Recombination map

2-82

RefSeq locus

NT_033778 REGION:17456472..17457129

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (8 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables dynein intermediate chain binding

inferred from direct assay

(Hall et al., 2010)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables dynein intermediate chain binding

inferred from biological aspect of ancestor with PANTHER:PTN000081506

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (3 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

involved_in dendrite morphogenesis

inferred from mutant phenotype

(Satoh et al., 2008)

involved_in microtubule-based movement

inferred from direct assay

(Bowman et al., 1999)

involved_in mushroom body development

inferred from mutant phenotype

(Reuter et al., 2003)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

involved_in dendrite morphogenesis

traceable author statement

(Gao and Bogert, 2003)

involved_in microtubule-based movement

inferred from biological aspect of ancestor with PANTHER:PTN000081506

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (4 terms)

Terms Based on Experimental Evidence (2 terms)

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from mutant phenotype

(Bowman et al., 1999)

part_of cytoplasmic dynein complex

inferred from mutant phenotype

(Bowman et al., 1999)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

is_active_in centrosome

inferred from biological aspect of ancestor with PANTHER:PTN000845699

(assigned by GO_Central )

(Gaudet et al., 2011)

part_of cytoplasmic dynein complex

inferred from biological aspect of ancestor with PANTHER:PTN000081506

(assigned by GO_Central )

(Gaudet et al., 2011)

is_active_in cytoplasm

inferred from biological aspect of ancestor with PANTHER:PTN000081506

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

DYNEIN LIGHT CHAINS

Protein Family (UniProt)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

DYNEIN LIGHT CHAINS -: Dyneins are minus end-directed microtubule motor protein complexes. Dynein light chains are involved in dynein complex assembly and interact with specific dynein adaptor proteins. (Adapted from PMID:19935668 and PMID:24064538).

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\robl for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure New Section

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry Q7KMS3)

If you don't see the viewer to the right, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.50

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

robl-RA

FBtr0086920

NM_079047

527

Additional Transcript Data and Comments

Reported size (kB)

0.504 (longest cDNA)

(Bowman et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

robl-PA

FBpp0086076

10.8

6.52

NP_523771

AAF57861

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

97 (aa); 12 (kD observed)

(Bowman et al., 1999)

Comments

External Data

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\robl using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

robl_1

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

(Bowman et al., 1999)

larval stage

(Bowman et al., 1999)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

part_of axonemal dynein complex

inferred from mutant phenotype

(Bowman et al., 1999)

part_of cytoplasmic dynein complex

inferred from mutant phenotype

(Bowman et al., 1999)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\robl in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\robl

View exonic expression by tissue for Dmel\robl

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36963/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0024196

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0024196

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0024196

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10751

Flygut - An atlas of the Drosophila adult midgut

FBgn0024196

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 7 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 8 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of robl

Transgenic constructs containing regulatory region of robl

Aberrations (Deficiencies and Duplications) ( 3 )

Inferred from experimentation ( 3 )

Gene disrupted in

Df(2R)k10408

(Bowman et al., 1999)

Df(2R)robl-b

(Bowman, 2000.3.30)

Df(2R)robl-c

(Bowman, 2000.3.30, Bowman et al., 1999)

Inferred from location ( 0 )

Df(2R)BSC161

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal - all die before end of larval stage | recessive

robl^Z

partially lethal - majority die, with Scer\GAL4^pnr-MD237

robl^GD12965

viable

robl^f03905

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

robl^GD12965

viable, with Scer\GAL4^Mef2.PR

robl^GD12965

viable, with Scer\GAL4^tin.CΔ4

robl^GD12965

Sterility

Allele

fertile

robl^f03905

Other Phenotypes

Allele

abnormal locomotor behavior | larval stage

robl^Z

abnormal mitotic cell cycle | larval stage

robl^Z

abnormal neuroanatomy | recessive | somatic clone | third instar larval stage

robl^MB

abnormal neuroanatomy | somatic clone

abnormal size | larval stage

robl^Z

abnormal size | pupal stage

robl^Z

decreased cell number | somatic clone

decreased occurrence of cell division | larval stage

robl^Z

paralytic | larval stage | posterior | recessive

robl^Z

some die during larval stage | recessive

robl^Z

some die during pupal stage, with Scer\GAL4^pnr-MD237

robl^GD12965

visible, with Scer\GAL4^pnr-MD237

robl^GD12965

wild-type

robl^+t6.6

Phenotype manifest in

Allele

adult mushroom body | somatic clone

axon | larval stage

robl^Z

dendrite | somatic clone

robl^Z

dendrite | third instar larval stage

robl^MB

embryonic/larval brain | larval stage

robl^Z

embryonic/larval mushroom body | somatic clone

eye

robl^Z

imaginal disc

robl^Z

imaginal disc | larval stage

robl^Z

larval multidendritic class IV neuron | somatic clone | third instar larval stage

robl^MB

larval segmental nerve

robl^Z

macrochaeta

robl^Z

mitosis & nuclear chromosome

robl^Z

mitotic anaphase

robl^Z

mitotic cell cycle

robl^Z

mitotic telophase

robl^Z

trichogen cell, with Scer\GAL4^pnr-MD237

robl^GD12965

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\DYNLRB2

14 of 15

Yes

Hsap\DYNLRB1

10 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (2)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Dynlrb2

14 of 15

Yes

Mmus\Dynlrb1

8 of 15

Yes

Rattus norvegicus (Norway rat) (2)

Rnor\Dynlrb2

12 of 13

Yes

Rnor\Dynlrb1

7 of 13

Yes

Xenopus tropicalis (Western clawed frog) (2)

Xtro\dynlrb2

12 of 12

Yes

Xtro\dynlrb1

8 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\dynlrb1

10 of 15

Yes

Drer\dynlrb2

9 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\dyrb-1

12 of 15

Yes

Arabidopsis thaliana (thale-cress) (0)

No records found.

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Other Organism Orthologs (via OrthoDB)

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (8)

Gene Symbol

Score

Source

Alignment

6 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

4 of 10

3 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

robl^K

ameliorates Alzheimer's disease

modeled by Hsap\APP^{695SPA4CT.UAS.Tag:MYC}

(Gunawardena and Goldstein, 2001)

modeled by Hsap\APP^{V717F.LOND.UAS}

(Gunawardena and Goldstein, 2001)

modeled by Hsap\APP^{695-Swedish.UAS}

(Gunawardena and Goldstein, 2001)

modeled by Hsap\APP^695.T.UAS

(Gunawardena and Goldstein, 2001)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

DYNLRB2; dynein light chain roadblock-type 2

14 of 15

607168

DYNLRB1; dynein light chain roadblock-type 1

10 of 15

607167

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

robl - CG13074

two hybrid

(Hu et al., 2017.6.13)

robl - Dlc90F

molecular sieving, predetermined participant

(Hall et al., 2010)

robl - Pur-α

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

robl - Trf

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

robl - ctp

molecular sieving, predetermined participant

(Hall et al., 2010)

robl - grh

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

robl - h

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

robl - robl37BC

experimental knowledge based

(Guruharsha et al., 2011)

robl - sw

x-ray crystallography, predetermined participant, molecular sieving, experimental knowledge based, isothermal titration calorimetry

(Guruharsha et al., 2011, Hall et al., 2010)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Appl

suppressible

robl

(Gunawardena and Goldstein, 2001)

CycB

enhanceable

robl

(Ji et al., 2002)

External Data

Linkouts

BioGRID - A database of protein and genetic interactions.

62657

DroID - A comprehensive database of gene and protein interactions.

FBgn0024196

MIST (protein-protein) - An integrated Molecular Interaction Database

robl

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0024196

External Data

Linkouts

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-82

Cytogenetic map

54B16-54B16

Sequence location

2R:17,456,472..17,457,129 [+]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

54B16-54B16

Limits computationally determined from genome sequence between P{EP}l(2)k01209^EP2177&P{lacW}k11012a and P{lacW}l(2)k07433^k07433

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

54B-54B

(Bowman, 1999.4.9)

54C-54C

(Bowman et al., 1998)

Experimentally Determined Recombination Data

Location

2-82

(FlyBase, 2016)

2-86.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (8)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

18710

w¹¹¹⁸; PBac{WH}robl^f03905

28484

y¹ w^*; P{EP}robl^G7617/CyO

8787

w^*; P{FRT(w^hs)}G13 robl^MB/CyO

31977

y¹ v¹; P{TRiP.JF03411}attP2/TM3, Sb¹

8803

y¹ w^*; P{FRT(w^hs)}G13 robl^B/CyO, y⁺

Kyoto

123236

w¹¹¹⁸; P{RS3}robl^CB-0554-3

VDRC

v105760

P{KK109229}VIE-260B

v22760

w¹¹¹⁸; P{GD12965}v22760/TM3

Genomic Clones (4)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (37)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

RE64145

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0024196

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0024196

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36963

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Bowman et al., 1999)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for database merge of

Source for merge of: robl l(2)k10408

(Bowman et al., 1999)

Additional comments

Other Comments

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

Identification: 1 allele of robl has been identified in a screen to isolate genes required for normal neuronal morphogenesis in larval mushroom body neurons.

(Reuter et al., 2003)

Identification: Screen for new axonal transport mutants to identify components of the axonal transport process.

(Bowman et al., 1998)

Mutants display a larval distal sluggish phenotype and accumulations of motors and cargo in the larval segmental nerves. Mutants also show a strong mitotic defect. Strong alleles of robl show a loss of imaginal tissue growth and abnormal anaphase-telophase mitotic structures in larval brain squashes. Weaker alleles of robl show a rough eye phenotype.

(Bowman et al., 1998)

Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology.

(Roch et al., 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 35 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

36963

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

Q7KMS3

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Q7KMS3

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

Q7KMS3

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0024196

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

36963/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0024196

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

robl_1

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0024196

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0024196

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG10751

Flygut - An atlas of the Drosophila adult midgut

FBgn0024196

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0024196

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

36963

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG10751

MARRVEL_MODEL - MARRVEL (model organism gene)

36963

modMine - A data warehouse for the modENCODE project

FBgn0024196

Linkouts

BioGRID - A database of protein and genetic interactions.

62657

DroID - A comprehensive database of gene and protein interactions.

FBgn0024196

DRSC - Results frm RNAi screens

FBgn0024196

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0024196

FlyMine - An integrated database for Drosophila genomics

FBgn0024196

MIST (protein-protein) - An integrated Molecular Interaction Database

robl

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG10751

(Fukuoh et al., 2014, Ling and Salvaterra, 2011, Zhang et al., 2011, Zhang et al., 2010, Keleman et al., 2009.8.5, Perkins et al., 2009, Hughes et al., 2008, Goldstein and Gunawardena, 2000)

DLC

(Power et al., 2012, Reis et al., 2012, Gunawardena et al., 2003)

LC7

(Hall et al., 2010, Hall et al., 2009)

Roadblock

(Puñal et al., 2021)

l(2)k10408

(Spradling et al., 1999)

robl

(Miller and Posakony, 2020, White et al., 2020, Hansen et al., 2019, Hu et al., 2017.6.13, Copf, 2015, Copf, 2014, Iacobucci et al., 2014, Kang et al., 2014, Gunawardena et al., 2013, Reis et al., 2012, Ling and Salvaterra, 2011, Satoh et al., 2008, Pfister et al., 2006)

roblk

(Power et al., 2012)

Name Synonyms

Dynein light chain LC7

(Dix et al., 2013)

Roadblock

(Satoh et al., 2008)

dynein light chain

(Power et al., 2012, Reis et al., 2012, Hall et al., 2010)

dynein, light chain, roadblock-type 2

(Ling and Salvaterra, 2011)

roadblock