• Home
• Tools
  • Tools Overview
  • Query Tools

    
    • QuickSearch
    • QueryBuilder
    • TermLink
    • CytoSearch
    • RNA-Seq Search
    • Interactions Browser
    • ImageBrowse
    • Find A Person
    • Google™ FlyBase
  • Genomic/Map Tools

    
    • BLAST
    • GBrowse
    • GBrowse 2 Beta
    • CytoSearch
    • Feature Mapper
    • Aberrations Maps
    • Chromosome Maps
    • Coordinates Converter
  • Retrieve/Convert

    
    • Batch Download
    • Coordinates Converter
    • ID Converter
  • Data Submission

    
    • Fast-Track Your Paper
    • Submit Personal Comm
    • Add A New Person
    • Update Person Info
  • People

    
    • Find A Person
    • Add A New Person
    • Update Person Info
• Files
  • Files Overview
  • Current release
  • Archived releases
  • Map Conversion
  • FTP site:
  • Releases (FTP)
  • Genomes (FTP)
• Species
  • Phylogeny
  • Sequenced Species
  • Synteny Table
  • Color Illustrations
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All Resources
  • Model Organisms
  • Stock Collections
  • Vectors & Constructs
  • Other links:
  • BDGP
  • DGRC
  • DIS by issue
  • FlyExpress
  • Interactive Fly
  • modENCODE
  • Textpresso for Fly
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • FlyBase Guides
  • Video Tutorials
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Contact FlyBase
• Archives
  • Prior Release
  • Other Archives
  • Coordinate Converter

Gene Dmel\robl

General Information
Symbol	Dmel\robl	Species	D. melanogaster
Name	roadblock	Annotation symbol	CG10751
Feature type	protein_coding_gene	FlyBase ID	FBgn0024196
Gene Model Status	Current	Stock availability	10 publicly available
Also Known As	DLC, LC7
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	54B16-54B16	Sequence location	2R:13,343,977..13,344,634 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene roadblock is referred to in FlyBase by the symbol Dmel\robl (CG10751, FBgn0024196). It is a protein_coding_gene from Drosophila melanogaster. Based on sequence similarity, it is predicted to have molecular function: ATPase activity, coupled. There is experimental evidence that it is involved in the biological process: dendrite morphogenesis; mushroom body development. 12 alleles are reported. The phenotypes of these alleles are annotated with: segmental nerve; eye; mitotic telophase; trichogen cell; mitotic cell cycle; macrochaeta; mitotic anaphase; imaginal disc. It has one annotated transcript and one annotated polypeptide. Protein features are: Dynein light chain, roadblock-type; Dynein light chain-related. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult fat body, virgin spermatheca, adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1625632_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of robl. Gene sequence location is 2R:13343977..13344634.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
Update Feed	Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 54B16-54B16   Limits computationally determined from genome sequence between P{EP}l(2)k01209EP2177&P{lacW}k11012a and P{lacW}l(2)k07433k07433
Experimentally Determined Cytological Location
Cytogenetic map Notes References 54B-54B     (Bowman, 1999.4.9) 54C-54C     (Bowman et al., 1998)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\robl for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) robl-RA FBtr0086920  NM_079047   527   97
Additional Transcript Data & Comments
Reported size (kB)	0.504 (longest cDNA) (Bowman et al., 1999)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein robl-PA   FBpp0086076   10.8   97   6.52     NP_523771   AAF57861
Additional Polypeptide Data & Comments
Reported size (kDa)	97 (aa); 12 (kD observed) (Bowman et al., 1999)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Dynein light chain-related (IPR004942) Dynein light chain, roadblock-type (IPR016561)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF57861   AF141920 AAD45986   AF141921 AAD45987   AI061910   AY071587 AAL49209   BI481570   BI484967   BP546035   BP547605   BP548469   BP552588   BP555223   BP558242   CO181016   CO338351   EC076389   EC268004   EV582834   EV594678   FE863529   FE863684   FE863789   FE863844   FE863915   GH739104   GH739200   GH739236   GH740217   GH742024   GH742218   GH742302   GH742760   GH743556   GH763973   GH765083   GH765118   GH766377   GH769016   GH769250   GH779754   GO254541
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	Q7KMS3
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage     (Bowman et al., 1999) larval stage     (Bowman et al., 1999)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: High or moderate levels of expression observed in all larval and adult organs/tissues. Expression at high levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male reproductive system, larval carcass. Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult fat body, virgin spermatheca, adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    799.6 Larval Midgut    712.6 Larval Hindgut    613 Larval Malpighian Tubules    652.6 Larval Fat Body    425.6 Larval Salivary Gland    1178.4 Larval Trachea    1186.775 Larval Carcass    522.075 Adult Head    668.3 Adult Eye    903.925 Adult Brain    689 Adult Thoracic-Abdominal Ganglion    803 Adult Crop    635.1 Adult Midgut    846.9 Adult Hindgut    779.2 Adult Malpighian Tubules    542.9 Adult Fat Body    329.8 Adult Salivary Gland    1016.6 Adult Heart    595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca    677.1 Adult Ovary    576.6 Adult Testis    557 Adult Male Accessory Gland    582.9 Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  (799.6) Larval Midgut  (712.6) Larval Hindgut  (613) Larval Malpighian Tubules  (652.6) Larval Fat Body    425.6 Larval Salivary Gland  (1178.4) Larval Trachea  (1186.775) Larval Carcass  522.075 Adult Head  (668.3) Adult Eye  (903.925) Adult Brain  (689) Adult Thoracic-Abdominal Ganglion  (803) Adult Crop  (635.1) Adult Midgut  (846.9) Adult Hindgut  (779.2) Adult Malpighian Tubules  (542.9) Adult Fat Body    329.8 Adult Salivary Gland  (1016.6) Adult Heart  (595.475) Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca  (677.1) Adult Ovary  (576.6) Adult Testis  (557) Adult Male Accessory Gland  (582.9) Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    799.6 Larval Midgut    712.6 Larval Hindgut    613 Larval Malpighian Tubules    652.6 Larval Fat Body    425.6 Larval Salivary Gland  (1178.4) Larval Trachea  (1186.775) Larval Carcass    522.075 Adult Head    668.3 Adult Eye    903.925 Adult Brain    689 Adult Thoracic-Abdominal Ganglion    803 Adult Crop    635.1 Adult Midgut    846.9 Adult Hindgut    779.2 Adult Malpighian Tubules    542.9 Adult Fat Body    329.8 Adult Salivary Gland  1016.6 Adult Heart    595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca    677.1 Adult Ovary    576.6 Adult Testis    557 Adult Male Accessory Gland    582.9 Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    799.6 Larval Midgut    712.6 Larval Hindgut    613 Larval Malpighian Tubules    652.6 Larval Fat Body    425.6 Larval Salivary Gland    1178.4 Larval Trachea    1186.775 Larval Carcass    522.075 Adult Head    668.3 Adult Eye    903.925 Adult Brain    689 Adult Thoracic-Abdominal Ganglion    803 Adult Crop    635.1 Adult Midgut    846.9 Adult Hindgut    779.2 Adult Malpighian Tubules    542.9 Adult Fat Body    329.8 Adult Salivary Gland    1016.6 Adult Heart    595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca    677.1 Adult Ovary    576.6 Adult Testis    557 Adult Male Accessory Gland    582.9 Adult Carcass    336.1 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    799.6 Larval Midgut    712.6 Larval Hindgut    613 Larval Malpighian Tubules    652.6 Larval Fat Body    425.6 Larval Salivary Gland    1178.4 Larval Trachea    1186.775 Larval Carcass    522.075 Adult Head    668.3 Adult Eye    903.925 Adult Brain    689 Adult Thoracic-Abdominal Ganglion    803 Adult Crop    635.1 Adult Midgut    846.9 Adult Hindgut    779.2 Adult Malpighian Tubules    542.9 Adult Fat Body    329.8 Adult Salivary Gland    1016.6 Adult Heart    595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca    677.1 Adult Ovary    576.6 Adult Testis    557 Adult Male Accessory Gland    582.9 Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System  799.6 Larval Midgut  712.6 Larval Hindgut  613 Larval Malpighian Tubules  652.6 Larval Fat Body    425.6 Larval Salivary Gland  (1178.4) Larval Trachea  (1186.775) Larval Carcass  522.075 Adult Head  668.3 Adult Eye  (903.925) Adult Brain  689 Adult Thoracic-Abdominal Ganglion  803 Adult Crop  635.1 Adult Midgut  (846.9) Adult Hindgut  779.2 Adult Malpighian Tubules  542.9 Adult Fat Body    329.8 Adult Salivary Gland  (1016.6) Adult Heart  595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca  677.1 Adult Ovary  576.6 Adult Testis  557 Adult Male Accessory Gland  582.9 Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    799.6 Larval Midgut    712.6 Larval Hindgut    613 Larval Malpighian Tubules    652.6 Larval Fat Body    425.6 Larval Salivary Gland  1178.4 Larval Trachea  1186.775 Larval Carcass    522.075 Adult Head    668.3 Adult Eye    903.925 Adult Brain    689 Adult Thoracic-Abdominal Ganglion    803 Adult Crop    635.1 Adult Midgut    846.9 Adult Hindgut    779.2 Adult Malpighian Tubules    542.9 Adult Fat Body    329.8 Adult Salivary Gland  1016.6 Adult Heart    595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca    677.1 Adult Ovary    576.6 Adult Testis    557 Adult Male Accessory Gland    582.9 Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    799.6 Larval Midgut    712.6 Larval Hindgut    613 Larval Malpighian Tubules    652.6 Larval Fat Body    425.6 Larval Salivary Gland    1178.4 Larval Trachea    1186.775 Larval Carcass    522.075 Adult Head    668.3 Adult Eye    903.925 Adult Brain    689 Adult Thoracic-Abdominal Ganglion    803 Adult Crop    635.1 Adult Midgut    846.9 Adult Hindgut    779.2 Adult Malpighian Tubules    542.9 Adult Fat Body    329.8 Adult Salivary Gland    1016.6 Adult Heart    595.475 Adult VirginFemale Spermatheca    466 Adult InseminatedFemale Spermatheca    677.1 Adult Ovary    576.6 Adult Testis    557 Adult Male Accessory Gland    582.9 Adult Carcass    336.1 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    668.3   NA  Eye    903.925   NA  Brain    689   799.6  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    803   NA  Crop    635.1   712.6  Midgut    846.9   613  Hindgut    779.2   652.6  Malpighian Tubules    542.9   425.6  Fat Body    329.8   1178.4  Salivary Gland    1016.6   NA  Heart    595.475   1186.775  Trachea    NA   NA  VirginFemale Spermatheca    466   NA  InseminatedFemale Spermatheca    677.1   NA  Ovary    576.6   NA  Testis    557   NA  Male Accessory Gland    582.9   522.075  Carcass    336.1 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    45 central nervous system, larvae L3    49 central nervous system, pupae P8    32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    44 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    58 digestive system, larvae L3 wandering    72 digestive system, 1-day adult    37 digestive system, 4-day adult    31 digestive system, 20-day adult    35 fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8    42 carcass, larvae L3 wandering    43 carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female    47 ovary, mated 4-day female    39 testis, mated 4-day male    112 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (45) central nervous system, larvae L3  (49) central nervous system, pupae P8  (32) head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male  (29) head, mated 4-day male  (33) head, mated 20-day male  (44) salivary gland, larvae L3 wandering  (34) salivary gland, white prepupae  (58) digestive system, larvae L3 wandering  (72) digestive system, 1-day adult  (37) digestive system, 4-day adult  (31) digestive system, 20-day adult  (35) fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8  (42) carcass, larvae L3 wandering  (43) carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female  (47) ovary, mated 4-day female  (39) testis, mated 4-day male  (112) accessory gland, mated 4-day male  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    45 central nervous system, larvae L3    49 central nervous system, pupae P8    32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    44 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    58 digestive system, larvae L3 wandering    72 digestive system, 1-day adult    37 digestive system, 4-day adult    31 digestive system, 20-day adult    35 fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8    42 carcass, larvae L3 wandering    43 carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female    47 ovary, mated 4-day female    39 testis, mated 4-day male  (112) accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    45 central nervous system, larvae L3    49 central nervous system, pupae P8    32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    44 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    58 digestive system, larvae L3 wandering    72 digestive system, 1-day adult    37 digestive system, 4-day adult    31 digestive system, 20-day adult    35 fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8    42 carcass, larvae L3 wandering    43 carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female    47 ovary, mated 4-day female    39 testis, mated 4-day male    112 accessory gland, mated 4-day male    34 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    45 central nervous system, larvae L3    49 central nervous system, pupae P8    32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    44 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    58 digestive system, larvae L3 wandering    72 digestive system, 1-day adult    37 digestive system, 4-day adult    31 digestive system, 20-day adult    35 fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8    42 carcass, larvae L3 wandering    43 carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female    47 ovary, mated 4-day female    39 testis, mated 4-day male    112 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (45) central nervous system, larvae L3  (49) central nervous system, pupae P8  32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male  29 head, mated 4-day male  (33) head, mated 20-day male  (44) salivary gland, larvae L3 wandering  (34) salivary gland, white prepupae  (58) digestive system, larvae L3 wandering  (72) digestive system, 1-day adult  (37) digestive system, 4-day adult  31 digestive system, 20-day adult  (35) fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8  (42) carcass, larvae L3 wandering  (43) carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female  (47) ovary, mated 4-day female  (39) testis, mated 4-day male  (112) accessory gland, mated 4-day male  (34) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    45 central nervous system, larvae L3    49 central nervous system, pupae P8    32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    44 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    58 digestive system, larvae L3 wandering    72 digestive system, 1-day adult    37 digestive system, 4-day adult    31 digestive system, 20-day adult    35 fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8    42 carcass, larvae L3 wandering    43 carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female    47 ovary, mated 4-day female    39 testis, mated 4-day male  112 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    45 central nervous system, larvae L3    49 central nervous system, pupae P8    32 head, virgin 1-day female    23 head, virgin 4-day female    22 head, virgin 20-day female    19 head, mated 1-day female    19 head, mated 4-day female    20 head, mated 20-day female    20 head, mated 1-day male    29 head, mated 4-day male    33 head, mated 20-day male    44 salivary gland, larvae L3 wandering    34 salivary gland, white prepupae    58 digestive system, larvae L3 wandering    72 digestive system, 1-day adult    37 digestive system, 4-day adult    31 digestive system, 20-day adult    35 fat body, larvae L3 wandering    5 fat body, white prepupae    24 fat body, pupae P8    42 carcass, larvae L3 wandering    43 carcass, 1-day adult    18 carcass, 4-day adult    19 carcass, 20-day adult    20 ovary, virgin 4-day female    47 ovary, mated 4-day female    39 testis, mated 4-day male    112 accessory gland, mated 4-day male    34 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of moderately high expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    80 embryo 02-04hr    112 embryo 04-06hr    169 embryo 06-08hr    128 embryo 08-10hr    141 embryo 10-12hr    213 embryo 12-14hr    109 embryo 14-16hr    95 embryo 16-18hr    84 embryo 18-20hr    61 embryo 20-22hr    62 embryo 22-24hr    70 larva L1    76 larva L2    72 larva L3 12hr old    51 larva L3 puffstage 1-2    61 larva L3 puffstage 3-6    67 larva L3 puffstage 7-9    96 white prepupae new    89 white prepupae 12hr    91 white prepupae 24hr    100 pupae 2d postWPP    87 pupae 3d postWPP    63 pupae 4d postWPP    60 adult male 01day    61 adult male 05day    64 adult male 30day    78 adult female 01day    63 adult female 05day    72 adult female 30day    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (80) embryo 02-04hr  (112) embryo 04-06hr  (169) embryo 06-08hr  (128) embryo 08-10hr  (141) embryo 10-12hr  (213) embryo 12-14hr  (109) embryo 14-16hr  (95) embryo 16-18hr  (84) embryo 18-20hr  (61) embryo 20-22hr  (62) embryo 22-24hr  (70) larva L1  (76) larva L2  (72) larva L3 12hr old  (51) larva L3 puffstage 1-2  (61) larva L3 puffstage 3-6  (67) larva L3 puffstage 7-9  (96) white prepupae new  (89) white prepupae 12hr  (91) white prepupae 24hr  (100) pupae 2d postWPP  (87) pupae 3d postWPP  (63) pupae 4d postWPP  (60) adult male 01day  (61) adult male 05day  (64) adult male 30day  (78) adult female 01day  (63) adult female 05day  (72) adult female 30day  (69) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    80 embryo 02-04hr  (112) embryo 04-06hr  (169) embryo 06-08hr  (128) embryo 08-10hr  (141) embryo 10-12hr  (213) embryo 12-14hr  (109) embryo 14-16hr    95 embryo 16-18hr    84 embryo 18-20hr    61 embryo 20-22hr    62 embryo 22-24hr    70 larva L1    76 larva L2    72 larva L3 12hr old    51 larva L3 puffstage 1-2    61 larva L3 puffstage 3-6    67 larva L3 puffstage 7-9    96 white prepupae new    89 white prepupae 12hr    91 white prepupae 24hr    100 pupae 2d postWPP    87 pupae 3d postWPP    63 pupae 4d postWPP    60 adult male 01day    61 adult male 05day    64 adult male 30day    78 adult female 01day    63 adult female 05day    72 adult female 30day    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    80 embryo 02-04hr    112 embryo 04-06hr    169 embryo 06-08hr    128 embryo 08-10hr    141 embryo 10-12hr    213 embryo 12-14hr    109 embryo 14-16hr    95 embryo 16-18hr    84 embryo 18-20hr    61 embryo 20-22hr    62 embryo 22-24hr    70 larva L1    76 larva L2    72 larva L3 12hr old    51 larva L3 puffstage 1-2    61 larva L3 puffstage 3-6    67 larva L3 puffstage 7-9    96 white prepupae new    89 white prepupae 12hr    91 white prepupae 24hr    100 pupae 2d postWPP    87 pupae 3d postWPP    63 pupae 4d postWPP    60 adult male 01day    61 adult male 05day    64 adult male 30day    78 adult female 01day    63 adult female 05day    72 adult female 30day    69 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    80 embryo 02-04hr    112 embryo 04-06hr    169 embryo 06-08hr    128 embryo 08-10hr    141 embryo 10-12hr    213 embryo 12-14hr    109 embryo 14-16hr    95 embryo 16-18hr    84 embryo 18-20hr    61 embryo 20-22hr    62 embryo 22-24hr    70 larva L1    76 larva L2    72 larva L3 12hr old    51 larva L3 puffstage 1-2    61 larva L3 puffstage 3-6    67 larva L3 puffstage 7-9    96 white prepupae new    89 white prepupae 12hr    91 white prepupae 24hr    100 pupae 2d postWPP    87 pupae 3d postWPP    63 pupae 4d postWPP    60 adult male 01day    61 adult male 05day    64 adult male 30day    78 adult female 01day    63 adult female 05day    72 adult female 30day    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (80) embryo 02-04hr  (112) embryo 04-06hr  (169) embryo 06-08hr  (128) embryo 08-10hr  (141) embryo 10-12hr  (213) embryo 12-14hr  (109) embryo 14-16hr  (95) embryo 16-18hr  (84) embryo 18-20hr  (61) embryo 20-22hr  (62) embryo 22-24hr  (70) larva L1  (76) larva L2  (72) larva L3 12hr old  (51) larva L3 puffstage 1-2  (61) larva L3 puffstage 3-6  (67) larva L3 puffstage 7-9  (96) white prepupae new  (89) white prepupae 12hr  (91) white prepupae 24hr  (100) pupae 2d postWPP  (87) pupae 3d postWPP  (63) pupae 4d postWPP  (60) adult male 01day  (61) adult male 05day  (64) adult male 30day  (78) adult female 01day  (63) adult female 05day  (72) adult female 30day  (69) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    80 embryo 02-04hr  112 embryo 04-06hr  (169) embryo 06-08hr  128 embryo 08-10hr  141 embryo 10-12hr  (213) embryo 12-14hr  109 embryo 14-16hr    95 embryo 16-18hr    84 embryo 18-20hr    61 embryo 20-22hr    62 embryo 22-24hr    70 larva L1    76 larva L2    72 larva L3 12hr old    51 larva L3 puffstage 1-2    61 larva L3 puffstage 3-6    67 larva L3 puffstage 7-9    96 white prepupae new    89 white prepupae 12hr    91 white prepupae 24hr    100 pupae 2d postWPP    87 pupae 3d postWPP    63 pupae 4d postWPP    60 adult male 01day    61 adult male 05day    64 adult male 30day    78 adult female 01day    63 adult female 05day    72 adult female 30day    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    80 embryo 02-04hr    112 embryo 04-06hr    169 embryo 06-08hr    128 embryo 08-10hr    141 embryo 10-12hr    213 embryo 12-14hr    109 embryo 14-16hr    95 embryo 16-18hr    84 embryo 18-20hr    61 embryo 20-22hr    62 embryo 22-24hr    70 larva L1    76 larva L2    72 larva L3 12hr old    51 larva L3 puffstage 1-2    61 larva L3 puffstage 3-6    67 larva L3 puffstage 7-9    96 white prepupae new    89 white prepupae 12hr    91 white prepupae 24hr    100 pupae 2d postWPP    87 pupae 3d postWPP    63 pupae 4d postWPP    60 adult male 01day    61 adult male 05day    64 adult male 30day    78 adult female 01day    63 adult female 05day    72 adult female 30day    69 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    278 Schneider line 2 Sg4    137 embryonic 1182-4H    55 embryonic GM2    102 embryonic Kc167    194 embryonic S1    88 embryonic S3    66 leg disc CME L1    110 wing disc CME-W2    176 wing disc ML-DmD8    85 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    57 wing disc ML-DmD21    108 wing disc ML-DmD32    41 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    107 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    88 CNS ML-DmBG2-c2    160 tumorous blood cells mbn2    82 ovary fGS/OSS    39 ovary OSC    61 ovary OSS    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (278) Schneider line 2 Sg4  (137) embryonic 1182-4H  (55) embryonic GM2  (102) embryonic Kc167  (194) embryonic S1  (88) embryonic S3  (66) leg disc CME L1  (110) wing disc CME-W2  (176) wing disc ML-DmD8  (85) wing disc ML-DmD9  (77) wing disc ML-DmD16-c3  (57) wing disc ML-DmD21  (108) wing disc ML-DmD32  (41) haltere disc ML-DmD17-c3  (81) eye-antennal disc ML-DmD11  (69) antennal disc ML-DmD20-c5  (107) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1  (88) CNS ML-DmBG2-c2  (160) tumorous blood cells mbn2  (82) ovary fGS/OSS  (39) ovary OSC  (61) ovary OSS  (29) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (278) Schneider line 2 Sg4  (137) embryonic 1182-4H    55 embryonic GM2  102 embryonic Kc167  (194) embryonic S1    88 embryonic S3    66 leg disc CME L1  (110) wing disc CME-W2  (176) wing disc ML-DmD8    85 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    57 wing disc ML-DmD21  (108) wing disc ML-DmD32    41 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5  107 mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1    88 CNS ML-DmBG2-c2  (160) tumorous blood cells mbn2    82 ovary fGS/OSS    39 ovary OSC    61 ovary OSS    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    278 Schneider line 2 Sg4    137 embryonic 1182-4H    55 embryonic GM2    102 embryonic Kc167    194 embryonic S1    88 embryonic S3    66 leg disc CME L1    110 wing disc CME-W2    176 wing disc ML-DmD8    85 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    57 wing disc ML-DmD21    108 wing disc ML-DmD32    41 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    107 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    88 CNS ML-DmBG2-c2    160 tumorous blood cells mbn2    82 ovary fGS/OSS    39 ovary OSC    61 ovary OSS    29 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    278 Schneider line 2 Sg4    137 embryonic 1182-4H    55 embryonic GM2    102 embryonic Kc167    194 embryonic S1    88 embryonic S3    66 leg disc CME L1    110 wing disc CME-W2    176 wing disc ML-DmD8    85 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    57 wing disc ML-DmD21    108 wing disc ML-DmD32    41 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    107 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    88 CNS ML-DmBG2-c2    160 tumorous blood cells mbn2    82 ovary fGS/OSS    39 ovary OSC    61 ovary OSS    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (278) Schneider line 2 Sg4  (137) embryonic 1182-4H  (55) embryonic GM2  (102) embryonic Kc167  (194) embryonic S1  (88) embryonic S3  (66) leg disc CME L1  (110) wing disc CME-W2  (176) wing disc ML-DmD8  (85) wing disc ML-DmD9  (77) wing disc ML-DmD16-c3  (57) wing disc ML-DmD21  (108) wing disc ML-DmD32  (41) haltere disc ML-DmD17-c3  (81) eye-antennal disc ML-DmD11  (69) antennal disc ML-DmD20-c5  (107) mixed discs ML-DmD4-c1  (110) CNS ML-DmBG1-c1  (88) CNS ML-DmBG2-c2  (160) tumorous blood cells mbn2  (82) ovary fGS/OSS  (39) ovary OSC  (61) ovary OSS  29 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+  (278) Schneider line 2 Sg4  137 embryonic 1182-4H    55 embryonic GM2  102 embryonic Kc167  (194) embryonic S1    88 embryonic S3    66 leg disc CME L1  110 wing disc CME-W2  (176) wing disc ML-DmD8    85 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    57 wing disc ML-DmD21  108 wing disc ML-DmD32    41 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5  107 mixed discs ML-DmD4-c1  110 CNS ML-DmBG1-c1    88 CNS ML-DmBG2-c2  (160) tumorous blood cells mbn2    82 ovary fGS/OSS    39 ovary OSC    61 ovary OSS    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    278 Schneider line 2 Sg4    137 embryonic 1182-4H    55 embryonic GM2    102 embryonic Kc167    194 embryonic S1    88 embryonic S3    66 leg disc CME L1    110 wing disc CME-W2    176 wing disc ML-DmD8    85 wing disc ML-DmD9    77 wing disc ML-DmD16-c3    57 wing disc ML-DmD21    108 wing disc ML-DmD32    41 haltere disc ML-DmD17-c3    81 eye-antennal disc ML-DmD11    69 antennal disc ML-DmD20-c5    107 mixed discs ML-DmD4-c1    110 CNS ML-DmBG1-c1    88 CNS ML-DmBG2-c2    160 tumorous blood cells mbn2    82 ovary fGS/OSS    39 ovary OSC    61 ovary OSS    29 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    30 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    57 Cadmium 50 mM 12 hrs, larvae L3    68 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    39 Ethanol 5% 3 hrs, larvae L3    54 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    36 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    37 Rotenone 2 μg 12 hrs, larvae L3    45 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult  (30) heat shock, 4-day adult  (34) Cadmium 50 mM 6 hrs, larvae L3  (57) Cadmium 50 mM 12 hrs, larvae L3  (68) Cadmium 50 mM 48 hrs, 4-day adult  (30) Cadmium 100 mM 48 hrs, 4-day adult  (35) Copper 0.5 mM 12 hrs, larvae L3  (37) Copper 15 mM 48 hrs, 4-day adult  (40) Zinc 5 mM 12 hrs, larvae L3  (28) Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3  (39) Ethanol 5% 3 hrs, larvae L3  (54) Ethanol 10% 3 hrs, larvae L3  (36) Caffeine 1.5 mg/ml 4 hrs, larvae L3  (32) Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (36) Caffeine 25 mg/ml 48 hrs, 4-day adult  26 Paraquat 5 mM 48 hrs, 4-day adult  (43) Paraquat 10 mM 48 hrs, 4-day adult  (37) Rotenone 2 μg 12 hrs, larvae L3  (45) Rotenone 8 μg 12 hrs, larvae L3  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    30 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    57 Cadmium 50 mM 12 hrs, larvae L3    68 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    39 Ethanol 5% 3 hrs, larvae L3    54 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    36 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    37 Rotenone 2 μg 12 hrs, larvae L3    45 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    30 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    57 Cadmium 50 mM 12 hrs, larvae L3    68 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    39 Ethanol 5% 3 hrs, larvae L3    54 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    36 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    37 Rotenone 2 μg 12 hrs, larvae L3    45 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    30 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    57 Cadmium 50 mM 12 hrs, larvae L3    68 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    39 Ethanol 5% 3 hrs, larvae L3    54 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    36 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    37 Rotenone 2 μg 12 hrs, larvae L3    45 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult  30 heat shock, 4-day adult  (34) Cadmium 50 mM 6 hrs, larvae L3  (57) Cadmium 50 mM 12 hrs, larvae L3  (68) Cadmium 50 mM 48 hrs, 4-day adult  30 Cadmium 100 mM 48 hrs, 4-day adult  (35) Copper 0.5 mM 12 hrs, larvae L3  (37) Copper 15 mM 48 hrs, 4-day adult  (40) Zinc 5 mM 12 hrs, larvae L3  28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3  (39) Ethanol 5% 3 hrs, larvae L3  (54) Ethanol 10% 3 hrs, larvae L3  (36) Caffeine 1.5 mg/ml 4 hrs, larvae L3  32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (36) Caffeine 25 mg/ml 48 hrs, 4-day adult  26 Paraquat 5 mM 48 hrs, 4-day adult  (43) Paraquat 10 mM 48 hrs, 4-day adult  (37) Rotenone 2 μg 12 hrs, larvae L3  (45) Rotenone 8 μg 12 hrs, larvae L3  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    30 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    57 Cadmium 50 mM 12 hrs, larvae L3    68 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    39 Ethanol 5% 3 hrs, larvae L3    54 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    36 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    37 Rotenone 2 μg 12 hrs, larvae L3    45 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    20 cold shock, 4-day adult    30 heat shock, 4-day adult    34 Cadmium 50 mM 6 hrs, larvae L3    57 Cadmium 50 mM 12 hrs, larvae L3    68 Cadmium 50 mM 48 hrs, 4-day adult    30 Cadmium 100 mM 48 hrs, 4-day adult    35 Copper 0.5 mM 12 hrs, larvae L3    37 Copper 15 mM 48 hrs, 4-day adult    40 Zinc 5 mM 12 hrs, larvae L3    28 Zinc 4.5 mM 48 hrs, 4-day adult    22 Ethanol 2.5% 3 hrs, larvae L3    39 Ethanol 5% 3 hrs, larvae L3    54 Ethanol 10% 3 hrs, larvae L3    36 Caffeine 1.5 mg/ml 4 hrs, larvae L3    32 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    36 Caffeine 25 mg/ml 48 hrs, 4-day adult    26 Paraquat 5 mM 48 hrs, 4-day adult    43 Paraquat 10 mM 48 hrs, 4-day adult    37 Rotenone 2 μg 12 hrs, larvae L3    45 Rotenone 8 μg 12 hrs, larvae L3    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_30 mE1_20_mRNA_expression_cluster_02
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024196 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10751-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | larval stage | recessive roblZ partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 roblGD12965 viable roblf03905 Sterility Allele fertile roblf03905 Other Phenotypes Allele decreased cell number | somatic clone roblB roblMB roblZ neuroanatomy defective | recessive | somatic clone | third instar larval stage roblMB neuroanatomy defective | somatic clone roblB roblMB roblZ paralytic | larval stage | posterior | recessive roblZ visible, with Scer\GAL4pnr-MD237 roblGD12965 wild-type robl+t6.6 Phenotype manifest in Allele adult mushroom body | somatic clone roblMB roblZ class IV dendritic arborizing neuron | somatic clone | third instar larval stage roblMB dendrite | somatic clone roblZ dendrite | third instar larval stage roblMB embryonic/larval mushroom body | somatic clone roblB roblMB eye roblZ imaginal disc roblZ macrochaeta roblZ mitosis & nuclear chromosome roblZ mitotic anaphase roblZ mitotic cell cycle roblZ mitotic telophase roblZ segmental nerve roblZ trichogen cell, with Scer\GAL4pnr-MD237 roblGD12965
Classical Alleles ( 7 )
For All Classical Alleles Show
Allele of robl Class Mutagen Stocks Known lesion roblf03905 piggyBac transposase 2 -- roblB 1 -- roblCB-0554-3 1 -- roblG7617 P-element activity 1 -- roblMB ethyl methanesulfonate 1 -- roblK 0 -- roblZ neomorphic allele - genetic evidence ethyl methanesulfonate 0 Yes
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of robl Class Mutagen Stocks Known lesion roblGD12965 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes roblJF03411 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes roblKK109229 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes robl+t6.6 in vitro construct - genomic fragment 0 Yes roblHsp70Bb.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)k10408 (Bowman et al., 1999) Df(2R)robl-b (Bowman, 2000.3.30) Df(2R)robl-c (Bowman et al., 1999, Bowman, 2000.3.30)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD12965} NA P{KK109229} NA P{TRiP.JF03411} NA heat-shock construct P{Hsp70Bb-robl.His6} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions P{RS3}roblCB-0554-3 NA PBac{WH}roblf03905 NA insertion of mobile activating element P{EP}roblG7617 NA
Gene Ontology: Function, Process & Cellular Component ( 7 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
dendrite morphogenesis inferred from mutant phenotype (Satoh et al., 2008) mushroom body development inferred from mutant phenotype (Reuter et al., 2003)
Cellular Component
CV term Evidence References
microtubule associated complex inferred from direct assay (Hughes et al., 2008)
Terms Based on Predictions or Assertions ( 6 terms )
Molecular Function
CV term Evidence References
ATPase activity, coupled inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Biological Process
CV term Evidence References
dendrite morphogenesis traceable author statement (Gao and Bogert, 2003) microtubule-based movement inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Cellular Component
CV term Evidence References
axonemal dynein complex non-traceable author statement (Bowman, 1999.4.9) dynein complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000) microtubule associated complex inferred from sequence or structural similarity (Goldstein and Gunawardena, 2000)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
robl - Aven anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Bacc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG10175 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG10465 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG10494 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG10602 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG10752 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG10909 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG11208 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG11791 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG13950 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG14020 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG14253 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG14980 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG15043 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG1983 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG2010 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG2091 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG30284 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG3061 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG31538 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG32236 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG32698 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG32758 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG3708 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG4866 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG4880 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG4953 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG5380 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG7550 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG7686 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG8323 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG8888 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CG9281 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CanB2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Cbp20 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Cen anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - CkIIβ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Cyp9f2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Dlc90F anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Ext2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Glycogenin anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - IntS12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Moe anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Obp19c anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Obp56e anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Or49b anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Pex12 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Rpn11 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - SNCF anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Sap47 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Smn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Spn47C anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Su(fu) anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Timp anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Trf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - Uev1A anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - ana2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - asrij anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - ct anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - dpr14 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - fng anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - frj anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - fwe anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - htl anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - imd anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - l(1)G0469 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - l(2)35Bc anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - pnut anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) robl - robl37BC experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) robl - sw experimental knowledge based (Guruharsha et al., 2011) robl - wgn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions robl allele Gene References roblK CycB (Ji et al., 2002)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 62657 DroID - A comprehensive database of gene and protein interactions. • FBgn0024196 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36963
Orthologs
OrthoDB Orthologs (45) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6BCF7R Dipteran inclusive ortholog search EOG6RXZ8T Insect inclusive ortholog search EOG67M0VT Arthropod inclusive ortholog search EOG6CNP81 Metazoa inclusive ortholog search EOG61CKS1
Orthologs in Drosophila Species (EOG6BCF7R)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\robl     Drosophila simulans   Dsim\GD11269 Y   Drosophila sechellia   Dsec\GM21776 Y   Drosophila sechellia   Dsec\GM26651     Drosophila erecta   Dere\GG20680 Y   Drosophila yakuba   Dyak\GE11664 Y   Drosophila ananassae   Dana\GF13122 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA10543 Y   Drosophila persimilis   Dper\GL11426 Y   Drosophila willistoni   Dwil\GK22899 Y   Drosophila virilis   Dvir\GJ20372 Y   Drosophila mojavensis   Dmoj\GI18578 Y   Drosophila grimshawi   Dgri\GH21399 Y
Orthologs in non-Drosophila Dipterans (EOG6RXZ8T)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL005354   Anopheles gambiae Malaria mosquito  Agam\AGAP003360   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ002048
Orthologs in non-Dipteran Insects (EOG67M0VT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\GB49522   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG022369   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15913   Atta cephalotes Leafcutter ant  Acep\ACEP10941   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14654   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL19338   Linepithema humile Argentine ant  Lhum\LH17839   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24029   Acyrthosiphon pisum Pea aphid  Apim\ACYPI004033   Bombyx mori Silkmoth  Bmor\BGIBMGA010715   Pediculus humanus Human body louse  Phum\PHUM551150   Tribolium castaneum Red flour beetle  Tcas\robl
Orthologs in non-Insect Arthropods (EOG6CNP81)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_257590   Ixodes scapularis Deer tick  Isca\ISCW002098
Orthologs in non-Arthropod Metazoa (EOG61CKS1)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\dyrb-1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_008699gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_003137gn Y Danio rerio Zebrafish  Drer\dynlrb1 Y Danio rerio Zebrafish  Drer\ENSDARG00000089952 Y Xenopus tropicalis Western clawed frog  Xtro\dynlrb1 Y Xenopus tropicalis Western clawed frog  Xtro\dynlrb2 Y Gallus gallus Domestic chicken  Ggal\DYNLRB2 Y Gallus gallus Domestic chicken  Ggal\DYNLRB1 Y Mus musculus House mouse  Mmus\Dynlrb2 Y Mus musculus House mouse  Mmus\Dynlrb1 Y Rattus norvegicus Norway rat  Rnor\Dynlrb2 Y Rattus norvegicus Norway rat  Rnor\Dynlrb1 Y Homo sapiens Human  Hsap\DYNLRB1 Y Homo sapiens Human  Hsap\DYNLRB2 Y
Human Orthologs (2)
Gene OMIM HGNC Hsap\DYNLRB1     Hsap\DYNLRB2
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD11269 Drosophila sechellia Dsec\GM21776 Drosophila erecta Dere\GG20680 Drosophila yakuba Dyak\GE11664 Drosophila ananassae Dana\GF13122 Drosophila pseudoobscura pseudoobscura Dpse\GA10543 Drosophila persimilis Dper\GL11426 Drosophila willistoni Dwil\GK22899 Drosophila virilis Dvir\GJ20372 Drosophila mojavensis Dmoj\GI18578 Drosophila grimshawi Dgri\GH21399
Stocks & Reagents
Stocks Listed in FlyBase ( 10 )
Bloomington	18710 w1118; PBac{WH}roblf03905 28484 y1 w*; P{EP}roblG7617/CyO 8787 w*; P{FRT(whs)}G13 roblMB/CyO 31977 y1 v1; P{TRiP.JF03411}attP2/TM3, Sb1 8803 y1 w*; P{FRT(whs)}G13 roblB/CyO, y+
Harvard	- PBac{WH}roblf03905
Kyoto	123236 w1118; P{RS3}roblCB-0554-3
VDRC	v105760 P{KK109229}VIE-260B v22760 w1118; P{GD12965}v22760/TM3 v22761 w1118; P{GD12965}v22761/CyO
Genomic Clones ( 1 )
	BACR21A09 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 37 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	RE64145
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD34974 RE68166
Other clones	103873 571308 Dmel_dig_020632_0658_1379 Dmel_neb_014018_0624_2158 EC13342 EN16557 FGH102B06 FGH118G04 FGH128B04 FGM219F04 FGM234B04 FGM313H03 RM07471 RM07771 RM07871 RM07971 RM08071 RP01095 RP001008763 RP001009876 RP001012508 RP001014382 RP001015530 RP001017193 RP001018625 RP001018808 RP001020013 RP001021475 RP001021480 RP001022715 RP001036223 RP001039663 RP001053640 RP001054055
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024196 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36963
Antibody Information
	polyclonal (Bowman et al., 1999)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of	Source for merge of: robl l(2)k10408 (Bowman et al., 1999)
Additional comments
Other Comments
	dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology. (Kiger et al., 2003) Identification: 1 allele of robl has been identified in a screen to isolate genes required for normal neuronal morphogenesis in larval mushroom body neurons. (Reuter et al., 2003) Identification: Screen for new axonal transport mutants to identify components of the axonal transport process. (Bowman et al., 1998) Mutants display a larval distal sluggish phenotype and accumulations of motors and cargo in the larval segmental nerves. Mutants also show a strong mitotic defect. Strong alleles of robl show a loss of imaginal tissue growth and abnormal anaphase-telophase mitotic structures in larval brain squashes. Weaker alleles of robl show a rough eye phenotype. (Bowman et al., 1998) Gene isolated in a screen of the second chromosome identifying mutants affecting disc morphology. (Roch et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_079047 RefSeq (Proteins) • NP_523771 DDBJ / EMBL / GenBank • AAD45986 AAD45987 AAF57861 AAL49209 AF141920 AF141921 AY071587 BI481570 Entrez Gene - A searchable database of RefSeq genes. • 36963 UniProtKB/TrEMBL • Q7KMS3
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Dynein light chain-related (IPR004942) Dynein light chain, roadblock-type (IPR016561)
Linkouts
	BioGRID - A database of protein and genetic interactions • 62657 DroID - A comprehensive database of gene and protein interactions. • FBgn0024196 DRSC - Results from RNAi screens. • FBgn0024196 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024196 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG10751-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024196 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36963 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 36963 modMine - Data generated by the modENCODE project. • FBgn0024196
Synonyms & Secondary IDs ( 9 )
Reported As
Symbol Synonym	CG10751 (Goldstein and Gunawardena, 2000, Keleman et al., 2009.8.5, Hughes et al., 2008, Perkins et al., 2009, Ling and Salvaterra, 2011) DLC (Gunawardena et al., 2003, Reis et al., 2012) l(2)k10408 (Spradling et al., 1999, ) LC7 (Hall et al., 2009, Hall et al., 2010) robl (Satoh et al., 2008, Ling and Salvaterra, 2011, Reis et al., 2012)
Name Synonym	dynein, light chain, roadblock-type 2 (Ling and Salvaterra, 2011) dynein light chain (Hall et al., 2010, Reis et al., 2012) roadblock (Bowman et al., 1998, Hall et al., 2010) Roadblock (Satoh et al., 2008)
Secondary FlyBase IDs
	FBgn0021950
References ( 42 )
Generate a list of	All references relating to this gene ( 42 )
List References by type	Research paper  ( 14 ) Supplementary material  ( 8 ) Review  ( 1 ) Personal communication to FlyBase  ( 10 ) Abstract  ( 3 ) FlyBase analysis  ( 1 ) DNA/RNA sequence record  ( 2 ) Curated genome annotation  ( 2 )
Recent research papers ( 3 )
	Díaz-Castillo and Ranz, 2012, Mol. Biol. Evol. 29(9): 2105--2108 Nuclear chromosome dynamics in the Drosophila male germ line contribute to the nonrandom genomic distribution of retrogenes. [FBrf0219274] Reis et al., 2012, Mol. Biol. Cell 23(9): 1700--1714 Molecular motor function in axonal transport in vivo probed by genetic and computational analysis in Drosophila. [FBrf0218178] Ling and Salvaterra, 2011, PLoS ONE 6(3): e17762 Robust RT-qPCR Data Normalization: Validation and Selection of Internal Reference Genes during Post-Experimental Data Analysis. [FBrf0213272] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...