Transposon inserted in intron
Gene model reviewed during 5.48
Low-frequency RNA-Seq exon junction(s) not annotated.
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\dnt using the Feature Mapper tool.
dnt transcript is detected at high levels in embryos, pupae and adults, but at low levels in larvae. In the blastoderm, expression is detected in a small anterior domain, and at 20-80% egg length. By gastrulation, dnt transcript is detected at the amnioproctodeal invagination, and later in the ventral and cephalic furrows. A striped pattern is seen at the start of gastrulation, and 14 stripes become prominent at extended germ band stage. The 14 stripes are just posterior to the domain of en protein expression. Expression is subsequently seen in doughnut shaped rings surrounding the developing tracheal pits. In late embryogenesis, expression is also detected in developing imaginal tissues. The patterns of dnt and os transcript expression show similarities.
In the precellular embryo, the maternal dnt transcript is ubiquitous. At the cellular blastoderm stage, the transcript is absent from the ends of the embryo, but is detected in a gap-like striped pattern. An ectodermal dynamic striped pattern of expression continues to be detected, especially in regions bordering epithelial invagination sites, including the ventral furrow, cephalic furrow, foregut, hindgut, optic lobe and tracheal pits.
GBrowse - Visual display of RNA-Seq signalsView Dmel\dnt in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: dnt CG17559
When dsRNA constructs are made and transiently transfected into S2 cells in RNAi experiments, a decrease in the ratio of cells in prometaphase and metaphase versus the total number of mitotic cells, a whole range of mitotic abnormalities, centrosome abnormalities, spindle abnormalities and chromosome abnormalities are seen.
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
The gene is named "doughnut" after part of the embryonic expression pattern, which is in doughnut-shaped rings surrounding the developing tracheal pits immediately prior to their invagination.