FB2025_02 , released April 17, 2025
Gene: Dmel\WASp
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General Information
Symbol
Dmel\WASp
Species
D. melanogaster
Name
WASp
Annotation Symbol
CG1520
Feature Type
protein_coding_gene
FlyBase ID
FBgn0024273
Gene Model Status
Current
Stock Availability
26 publicly available
Gene Summary
WASp (WASp) encodes the homolog of the conserved Wiskott-Aldrich Syndrome family of nucleation promoting factors. It enables the Arp2/3 complex to nucleate polymerization of branched microfilament arrays. It contributes to gastrulation, myoblast fusion, synapse morphology at neuromuscular junctions, sensory organ development, and spermatogenesis. [Date last reviewed: 2019-03-21] (FlyBase Gene Snapshot)
All Summaries
Also Known As

Wsp, Wiskott-Aldrich syndrome protein, D-WASP, dWASP

Key Links
Genomic Location
Cytogenetic map
98E5-98E5
Sequence location
Recombination map
3-97
RefSeq locus
NT_033777 REGION:28824506..28832024
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (32 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
enables protein binding
inferred from physical interaction with FLYBASE:Abi; FB:FBgn0020510
(Bogdan and Klambt, 2003)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables actin binding
inferred from electronic annotation with InterPro:IPR003124
(InterPro Project Members, 2004-)
enables small GTPase binding
inferred from biological aspect of ancestor with PANTHER:PTN008593182
(GO Reference Genome Project, 2011-)
Biological Process (23 terms)
Terms Based on Experimental Evidence (20 terms)
CV Term
Evidence
References
involved_in apical constriction
inferred from mutant phenotype
(Bertet et al., 2009)
involved_in Arp2/3 complex-mediated actin nucleation
inferred from direct assay
(Liu et al., 2009)
involved_in axonal fasciculation
inferred from genetic interaction with FLYBASE:SCAR; FB:FBgn0041781
(Zallen et al., 2002)
involved_in axonogenesis
inferred from genetic interaction with FLYBASE:SCAR; FB:FBgn0041781
(Zallen et al., 2002)
involved_in cell competition in a multicellular organism
inferred from mutant phenotype
(Li and Baker, 2007)
involved_in chaeta morphogenesis
inferred from mutant phenotype
(Zallen et al., 2002)
involved_in clathrin-dependent endocytosis
inferred from mutant phenotype
(Kochubey et al., 2006)
involved_in cuticle pattern formation
inferred from mutant phenotype
(Chanut-Delalande et al., 2006)
involved_in epidermal cell differentiation
inferred from mutant phenotype
(Chanut-Delalande et al., 2006)
involved_in intracellular protein transport
inferred from mutant phenotype
(Rajan et al., 2009)
involved_in microtubule bundle formation
inferred from direct assay
(Liu et al., 2009)
involved_in muscle attachment
inferred from mutant phenotype
(Schafer et al., 2007)
involved_in myoblast fusion
inferred from mutant phenotype
(Sens et al., 2010, Massarwa et al., 2007, Schafer et al., 2007)
inferred from genetic interaction with FLYBASE:Whamy; FB:FBgn0037750
(Brinkmann et al., 2016)
inferred from genetic interaction with FLYBASE:Arp3; FB:FBgn0262716
(Berger et al., 2008)
involved_in negative regulation of synaptic assembly at neuromuscular junction
inferred from mutant phenotype
(Nahm et al., 2010, Coyle et al., 2004)
involved_in regulation of actin filament polymerization
inferred from mutant phenotype
(Rotkopf et al., 2011, Bertet et al., 2009)
involved_in regulation of embryonic cell shape
inferred from mutant phenotype
(Bertet et al., 2009)
involved_in regulation of synaptic assembly at neuromuscular junction
inferred from direct assay
(Nahm et al., 2010)
involved_in sensory organ development
inferred from mutant phenotype
(Tal et al., 2002)
involved_in spermatid development
inferred from mutant phenotype
(Rotkopf et al., 2011)
involved_in vesicle budding from membrane
inferred from mutant phenotype
(Kochubey et al., 2006)
Terms Based on Predictions or Assertions (4 terms)
CV Term
Evidence
References
involved_in actin filament organization
inferred from electronic annotation with InterPro:IPR011026
(InterPro Project Members, 2004-)
involved_in asymmetric cell division
traceable author statement
(Vartiainen and Machesky, 2004)
involved_in cell fate commitment
traceable author statement
(Vartiainen and Machesky, 2004)
involved_in regulation of actin filament polymerization
inferred from biological aspect of ancestor with PANTHER:PTN008593182
(GO Reference Genome Project, 2011-)
Cellular Component (6 terms)
Terms Based on Experimental Evidence (6 terms)
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Rodriguez-Mesa et al., 2012)
located_in microvillus
inferred from mutant phenotype
(Zelhof and Hardy, 2004)
located_in neuromuscular junction
inferred from direct assay
(Nahm et al., 2010)
located_in nucleus
inferred from direct assay
(Rodriguez-Mesa et al., 2012)
part_of protein-containing complex
inferred from physical interaction with FLYBASE:Abi; FB:FBgn0020510
(Stephan et al., 2011)
located_in rhabdomere
inferred from mutant phenotype
(Zelhof and Hardy, 2004)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN008593182
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Summaries
Gene Snapshot
WASp (WASp) encodes the homolog of the conserved Wiskott-Aldrich Syndrome family of nucleation promoting factors. It enables the Arp2/3 complex to nucleate polymerization of branched microfilament arrays. It contributes to gastrulation, myoblast fusion, synapse morphology at neuromuscular junctions, sensory organ development, and spermatogenesis. [Date last reviewed: 2019-03-21]
Gene Group (FlyBase)
WISKOTT-ALDRICH SYNDROME PROTEINS -
The Wiskott-Aldrich Syndrome Proteins (WASP) are a family of nucleation-promoting factors. WASP proteins share a conserved Arp2/3-complex-activating C-terminal VCA/WCA (verprolin/WASP, cofilin, acidic) domain. WASP members participate in cytoskeleton organization, notably through the stimulation of the actin-nucleating activity of the Arp2/3 complex. (Adapted from FBrf0230860 and FBrf0217509).
Summary (Interactive Fly)

Wiskott-Aldrich syndrome protein - regulates actin polymerization - enables the Arp2/3 complex to nucleate polymerization of branched microfilament arrays and contributes to gastrulation, myoblast fusion, synapse morphology at neuromuscular junctions, sensory organ development, and spermatogenesis

Gene Model and Products
Number of Transcripts
4
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\WASp for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry Q9VAT0)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Gene model reviewed during 5.48

Low-frequency RNA-Seq exon junction(s) not annotated.

Annotated transcripts do not represent all supported alternative splices within 5' UTR.

Gene model reviewed during 5.55

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
WASp-RA
FBtr0085352
NM_143380
2794
527
WASp-RB
FBtr0085353
NM_170378
2682
527
WASp-RC
FBtr0085354
NM_176578
2500
494
WASp-RD
FBtr0336768
NM_001276116
2734
527
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
WASp-PA
FBpp0084721
57.0
527
9.29
Q9VAT0
NP_651637
AAF56819
WASp-PB
FBpp0084722
57.0
527
9.29
Q9VAT0
NP_733257
AAN14303
WASp-PC
FBpp0084723
53.6
494
9.75
Q86B74
NP_788755
AAO41609
WASp-PD
FBpp0307744
57.0
527
9.29
Q9VAT0
NP_001263045
AGB96425
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

527 aa isoforms: WASp-PA, WASp-PB, WASp-PD
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\WASp using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

1.20

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 14
fusion competent cell
(Jin et al., 2011)
embryonic stage 16
muscle attachment site
(Schafer et al., 2007)
third instar larval stage
embryonic/larval neuromuscular junction
(Nahm et al., 2010)
embryonic/larval neuromuscular junction
(Nahm et al., 2010)
wandering third instar larval stage
type I bouton
(Coyle et al., 2004)
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage
organism | ubiquitous
(Schafer et al., 2007)
embryonic stage 16
larval central nervous system
(Schafer et al., 2007)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data

WASp protein is expressed in fusion competent cells.

(Jin et al., 2011)

WASp is present at the postsynaptic domain of type I boutons at the neuromuscular junction.

(Nahm et al., 2010)

WASp is maternally expressed and is ubiquitously expressed during embryonic development. At the end of embryogenesis, WASp is clearly expressed at muscle attachment sites and in the CNS.

(Schafer et al., 2007)
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from direct assay
(Rodriguez-Mesa et al., 2012)
located_in microvillus
inferred from mutant phenotype
(Zelhof and Hardy, 2004)
located_in neuromuscular junction
inferred from direct assay
(Nahm et al., 2010)
located_in nucleus
inferred from direct assay
(Rodriguez-Mesa et al., 2012)
part_of protein-containing complex
inferred from physical interaction with FLYBASE:Abi; FB:FBgn0020510
(Stephan et al., 2011)
located_in rhabdomere
inferred from mutant phenotype
(Zelhof and Hardy, 2004)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\WASp in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 11 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 44 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of WASp
Transgenic constructs containing regulatory region of WASp
Aberrations (Deficiencies and Duplications) ( 2 )
Inferred from experimentation ( 2 )
Gene disrupted in
Df(3R)3450
(Schafer et al., 2007, Tal et al., 2002, Ben-Yaacov et al., 2001)
Df(3R)Exel6210
(Ryder, 2004.4.26)
Inferred from location ( 4 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(3R)3450)
lethal | embryonic stage | recessive | rescuable maternal effect
lethal | pharate adult stage | recessive
lethal | recessive
lethal - all die before end of P-stage (with WASp1)
lethal - all die before end of P-stage (with WASp3)
lethal - all die before end of P-stage | recessive
lethal - all die during embryonic stage
partially lethal
partially lethal (with Df(3R)3450)
partially lethal - majority die, with Scer\GAL4pnr-MD237
viable
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
fertile
male sterile, with Df(3R)3450/WASp1, Scer\GAL4arm.PS
male sterile, with Scer\GAL4A3.eya
male sterile (with Df(3R)3450), with Scer\GAL4arm.PS, WASpH242D.UAS
Other Phenotypes
Allele
abnormal behavior | recessive
abnormal cell shape | extended germ band stage, with Scer\GAL4VP16.mat.αTub67C
abnormal neuroanatomy
abnormal neuroanatomy, with Scer\GAL4C57
abnormal neuroanatomy | embryonic stage, with Scer\GAL4eve.RN2
abnormal neuroanatomy | larval stage | conditional, with Scer\GAL4A58
abnormal neuroanatomy | pupal stage, with Scer\GAL45-56
abnormal neuroanatomy | pupal stage (with Df(3R)3450)
abnormal neuroanatomy | third instar larval stage (with Df(3R)3450)
decreased rate of movement | recessive
decreased size | oogenesis
increased cell number | embryonic stage
increased cell number | embryonic stage, with Scer\GAL4Hand.PU
increased cell number | embryonic stage, with Scer\GAL4tin.CΔ4
increased cell number | embryonic stage (with Df(3R)3450)
increased cell number | pupal stage (with Df(3R)3450)
some die during embryonic stage
some die during pharate adult stage (with WASp1)
some die during pharate adult stage (with WASp3)
some die during pharate adult stage | recessive
some die during pupal stage, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4pnr-MD237
visible, with Scer\GAL4sca-537.4
visible (with Df(3R)3450)
wild-type
Phenotype manifest in
Allele
actin filament, with Df(3R)3450/WASp1, Scer\GAL4arm.PS
actin filament (with Df(3R)3450), with Scer\GAL4arm.PS, WASpH242D.UAS
actin filament & eye photoreceptor cell | somatic clone
adult abdominal sensillum (with Df(3R)3450)
adult brain, with Scer\GAL4elav.PU
adult head bristle (with Df(3R)3450)
adult thorax & microchaeta (with Df(3R)3450)
adult thorax & microchaeta & external sensory organ precursor cell IIa (with Df(3R)3450)
adult thorax & microchaeta & external sensory organ precursor cell IIb (with Df(3R)3450)
bouton
bouton | increased number
chaeta, with Scer\GAL4sca-537.4
dendrite | embryonic stage, with Scer\GAL4eve.RN2
denticle
dorsal acute muscle cell, with Scer\GAL4twi.PB
dorsal acute muscle cell (with Df(3R)3450)
dorsal acute muscle cell | embryonic stage
dorsocentral bristle (with Df(3R)3450)
ectoderm | extended germ band stage, with Scer\GAL4VP16.mat.αTub67C
embryonic/larval fat body | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
embryonic/larval neuromuscular junction | third instar larval stage (with Df(3R)3450)
embryonic cardioblast | increased number
embryonic cardioblast | increased number, with Scer\GAL4Hand.PU
embryonic cardioblast | increased number, with Scer\GAL4tin.CΔ4
embryonic myoblast
embryonic myoblast, with Scer\GAL4twi.PG
embryonic myoblast (with Df(3R)3450)
embryonic myoblast (with WASp1)
embryonic myoblast (with WASp3)
embryonic myoblast (with WASp3D3-035)
embryonic peripheral nervous system & external sensory organ | germ-line clone (with Df(3R)3450)
embryonic peripheral nervous system & neuron | supernumerary | germ-line clone (with Df(3R)3450)
external sensory organ | somatic clone
external sensory organ precursor cell | pupal stage (with Df(3R)3450)
external sensory organ precursor cell IIa | somatic clone
external sensory organ precursor cell IIb | somatic clone
extracellular matrix | third instar larval stage, with Scer\GAL4SPARC-MI00329-GAL4
eye disc | somatic clone
filamentous actin | embryonic stage
head cyst cell, with Df(3R)3450/WASp1, Scer\GAL4arm.PS
head cyst cell (with Df(3R)3450), with Scer\GAL4arm.PS, WASpH242D.UAS
interommatidial bristle (with Df(3R)3450)
interommatidial bristle & eo neuron | supernumerary (with Df(3R)3450)
interommatidial bristle & tormogen cell (with Df(3R)3450)
interommatidial bristle & trichogen cell (with Df(3R)3450)
interommatidial bristle | adult stage | somatic clone (with Df(3R)3450)
interommatidial bristle | somatic clone
larval DA1 motor neuron | embryonic stage, with Scer\GAL4eve.RN2
larval dorsal multidendritic neuron ddaC | larval stage | conditional, with Scer\GAL4A58
larval dorsal multidendritic neuron ddaC | pupal stage, with Scer\GAL45-56
larval RP2 motor neuron | increased number (with Df(3R)3450)
larval somatic muscle cell
larval somatic muscle cell, with Scer\GAL4twi.PB
larval somatic muscle cell, with Scer\GAL4twi.PG
larval tracheocyte | larval stage, with Scer\GAL4btl.PS
larval ventral nerve cord | rescuable maternal effect
lateral transverse muscle cell | late embryonic stage
leg & microchaeta (with Df(3R)3450)
macrochaeta (with Df(3R)3450)
macrochaeta & adult head | somatic clone
macrochaeta | immature adult stage
macrochaeta | immature adult stage (with Df(3R)3450)
macrochaeta | increased number (with Df(3R)3450)
mesothoracic bristle & eo neuron | supernumerary
mesothoracic bristle & tormogen cell
mesothoracic bristle & trichogen cell
microchaeta (with Df(3R)3450)
microchaeta & adult head | somatic clone
microvillus & eye photoreceptor cell | somatic clone
mitochondrion, with Scer\GAL4elav.PU
muscle attachment site
muscle attachment site (with Df(3R)3450)
myoblast, with Scer\GAL4twi.PB
myoblast | embryonic stage
neuromuscular junction, with Scer\GAL4C57
neuromuscular junction | larval stage
neuron | embryonic stage (with Df(3R)3450)
NMJ bouton, with Scer\GAL4C57
NMJ bouton | third instar larval stage (with Df(3R)3450)
notopleural bristle (with Df(3R)3450)
nucleus | embryonic stage
nurse cell ring canal
plasma membrane | embryonic stage
primordial germ cell | embryonic stage
primordial germ cell | embryonic stage (with WASp1)
primordial germ cell | embryonic stage (with WASp3)
rhabdomere (with WASp1)
rhabdomere (with WASp3)
rhabdomere | adult stage | somatic clone (with Df(3R)3450)
rhabdomere | somatic clone
salivary gland body primordium | embryonic stage, with Scer\GAL4fkh.PH
scutellar bristle (with Df(3R)3450)
seminal vesicle, with Df(3R)3450/WASp1, Scer\GAL4arm.PS
seminal vesicle, with Scer\GAL4A3.eya
seminal vesicle (with Df(3R)3450), with Scer\GAL4arm.PS, WASpH242D.UAS
sensory mother cell (with Df(3R)3450)
spermatid cyst, with Df(3R)3450/WASp1, Scer\GAL4arm.PS
spermatid cyst, with Scer\GAL4A3.eya
spermatid cyst (with Df(3R)3450), with Scer\GAL4arm.PS, WASpH242D.UAS
synaptic vesicle | third instar larval stage (with Df(3R)3450)
tormogen cell (with Df(3R)3450)
tracheocyte | P-stage, with Scer\GAL4btl.PS
trichogen cell, with Scer\GAL4pnr-MD237
trichogen cell (with Df(3R)3450)
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (5)
Hsap\WAS
11 of 14
Yes
Yes
Hsap\WASL
9 of 14
No
Yes
Hsap\CDC42SE1
1 of 14
No
No
1  
Hsap\CDC42SE2
1 of 14
No
No
Hsap\CEL
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Wasl
11 of 14
Yes
Yes
Rnor\Was
10 of 14
No
Yes
Rnor\Cdc42se1
1 of 14
No
No
Rnor\Cdc42se2
1 of 14
No
No
Mus musculus (laboratory mouse) (4)
Mmus\Was
11 of 14
Yes
Yes
Mmus\Wasl
9 of 14
No
Yes
Mmus\Cdc42se1
1 of 14
No
No
Mmus\Cdc42se2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (8)
Xtro\wasl
8 of 13
Yes
Yes
Xtro\was
3 of 13
No
Yes
Xtro\wasll
2 of 13
No
Yes
Xtro\cdc42se1
1 of 13
No
No
Xtro\cdc42se2l
1 of 13
No
No
Xtro\cdc42se2
1 of 13
No
No
Xtro\thul16
1 of 13
No
Yes
Xtro\XB5748694
1 of 13
No
Yes
Danio rerio (Zebrafish) (7)
Drer\wasb
11 of 14
Yes
Yes
Drer\wasa
10 of 14
No
Yes
Drer\wasla
10 of 14
No
Yes
Drer\waslb
10 of 14
No
Yes
Drer\si:dkey-197j19.6
4 of 14
No
Yes
Drer\cdc42se1
1 of 14
No
No
Drer\cdc42se2
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (6)
Cele\wsp-1
5 of 14
Yes
Yes
Cele\B0280.2
1 of 14
No
Yes
Cele\C31C9.6
1 of 14
No
Yes
Cele\F54A3.6
1 of 14
No
Yes
Cele\kin-23
1 of 14
No
No
Cele\viro-2
1 of 14
No
Yes
Anopheles gambiae (African malaria mosquito) (2)
Agam\AgaP_AGAP001081
10 of 12
Yes
Yes
Agam\AgaP_AGAP003263
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (0)
Saccharomyces cerevisiae (Brewer's yeast) (2)
Scer\LAS17
5 of 13
Yes
Yes
Scer\VRP1
1 of 13
No
No
Schizosaccharomyces pombe (Fission yeast) (2)
Spom\wsp1
3 of 12
Yes
Yes
Spom\vrp1
1 of 12
No
No
Escherichia coli (enterobacterium) (0)
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:WASp. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (2)
Whamy
2 of 13
Spec2
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 3 )
    Modifiers Based on Experimental Evidence ( 1 )
    Allele
    Disease
    Interaction
    References
    WASpUAS.cBa
    exacerbates  tauopathy
    modeled by Hsap\MAPTR406W.UAS
    (Duboff et al., 2012)
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    WAS; WASP actin nucleation promoting factor
    11 of 14
    300392
    WASL; WASP like actin nucleation promoting factor
    9 of 14
    605056
        Functional Complementation Data
        Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
        Interactions
        Summary of Physical Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please see the Physical Interaction reports below for full details
        protein-protein
        Physical Interaction
        Assay
        References
        WASp - Abi
        pull down, western blot, two hybrid, anti bait coimmunoprecipitation, molecular sieving
        (Kim et al., 2019, Stephan et al., 2011, Bogdan et al., 2005, Bogdan and Klambt, 2003)
        WASp - Abp1
        pull down, western blot
        (Koch et al., 2014)
        WASp - Amph
        pull down, western blot
        (Zelhof and Hardy, 2004)
        WASp - Cdc42
        enzymatic study, pull down, western blot, autoradiography, anti tag western blot, fluorescent resonance energy transfer, fluorescence microscopy
        (Sharifai et al., 2014, Nahm et al., 2010, Fricke et al., 2009, Liu et al., 2009, Rodal et al., 2008)
        WASp - Cip4
        pull down, western blot, two hybrid, anti tag western blot, anti tag coimmunoprecipitation, anti bait coimmunoprecipitation, bimolecular fluorescence complementation, fluorescence microscopy
        (Zobel et al., 2015, Nahm et al., 2010, Fricke et al., 2009, Leibfried et al., 2008)
        WASp - Crk
        pull down, anti tag western blot
        (Kim et al., 2007)
        WASp - Cyfip
        pull down, western blot
        (Bogdan et al., 2005)
        WASp - Hem
        pull down, western blot
        (Bogdan et al., 2005)
        WASp - SCAR
        pull down, western blot, anti bait coimmunoprecipitation
        (Bogdan et al., 2005)
        WASp - SH3PX1
        two hybrid, anti tag coimmunoprecipitation, western blot, pull down
        (Worby et al., 2002, Worby et al., 2001)
        WASp - Synd
        pull down, western blot
        (Kumar et al., 2009)
        WASp - Vrp1
        anti tag coimmunoprecipitation, anti tag western blot, experimental knowledge based, western blot, pull down
        (Kaipa et al., 2013, Guruharsha et al., 2011, Jin et al., 2011, Kim et al., 2007)
        WASp - Whamy
        pull down, molecular weight estimation by staining, anti tag coimmunoprecipitation, anti tag western blot
        (Brinkmann et al., 2016)
        WASp - dock
        two hybrid, anti tag coimmunoprecipitation, anti tag western blot
        (Kaipa et al., 2013, Worby et al., 2001)
        WASp - nwk
        pull down, western blot, two hybrid, enzymatic study
        (Stanishneva-Konovalova et al., 2016, Rodal et al., 2008, Coyle et al., 2004)
        Summary of Genetic Interactions
        Interaction Browsers
        View in FlyBase Interactions Browser   View in MIST tool (external link)
        Please look at the allele data for full details of the genetic interactions
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        WASp
        suppressible
        AP-1μ
        (Benhra et al., 2011)
        WASp
        enhanceable
        Arp3
        (Berger et al., 2008)
        WASp
        enhanceable
        Cdc42|Cip4
        (Nahm et al., 2010)
        WASp
        enhanceable
        Cip4
        (Nahm et al., 2010)
        WASp
        suppressible
        H
        (Ben-Yaacov et al., 2001)
        WASp
        enhanceable
        N
        (Ben-Yaacov et al., 2001)
        WASp
        suppressible
        N
        (Ben-Yaacov et al., 2001)
        WASp
        enhanceable
        nwk
        (Khuong et al., 2010, Coyle et al., 2004)
        WASp
        enhanceable
        PIP5K59B
        (Kim et al., 2021)
        WASp
        suppressible
        RhoGAP92B
        (Nahm et al., 2010)
        WASp
        enhanceable
        Whamy
        (Brinkmann et al., 2016)
        WASp
        suppressible
        Whamy
        (Brinkmann et al., 2016)
        WASp
        suppressible
        wit
        (Nahm et al., 2010)
        Starting gene(s)
        Interaction type
        Interacting gene(s)
        Reference
        Abi
        suppressible
        WASp
        (Bogdan et al., 2005)
        Arp3
        enhanceable
        WASp
        (Berger et al., 2008)
        Cdc42
        enhanceable
        WASp|Cip4
        (Nahm et al., 2010)
        cindr
        enhanceable
        WASp
        (Johnson et al., 2008)
        Cip4
        enhanceable
        WASp
        (Nahm et al., 2010)
        Cip4
        enhanceable
        Cdc42|WASp
        (Nahm et al., 2010)
        Cip4
        suppressible
        WASp
        (Fricke et al., 2009)
        Cyfip
        suppressible
        WASp
        (Bogdan et al., 2004)
        dock
        suppressible
        WASp
        (Stark et al., 2021)
        fog
        suppressible
        WASp
        (Ratnaparkhi, 2013)
        Hem
        suppressible
        WASp
        (Schafer et al., 2007, Bogdan and Klambt, 2003)
        nwk
        enhanceable
        WASp
        (Khuong et al., 2010, Coyle et al., 2004)
        Pak3
        enhanceable
        WASp
        (Duan et al., 2012)
        RhoGAP92B
        suppressible
        WASp
        (Nahm et al., 2010)
        SCAR
        enhanceable
        WASp
        (Berger et al., 2008)
        sn|m|f
        enhanceable
        WASp
        (Chanut-Delalande et al., 2006)
        upd1
        suppressible
        WASp
        (Bertet et al., 2009)
        Vrp1
        suppressible
        WASp
        (Massarwa et al., 2007)
        Vrp1
        enhanceable
        WASp
        (Kaipa et al., 2013)
        wit
        suppressible
        WASp
        (Khuong et al., 2010)
        External Data
        Linkouts
        BioGRID - A database of protein and genetic interactions.
        DroID - A comprehensive database of gene and protein interactions.
        MIST (genetic) - An integrated Molecular Interaction Database
        MIST (protein-protein) - An integrated Molecular Interaction Database
        Pathways
        Signaling Pathways (FlyBase)
        Metabolic Pathways
        FlyBase
        External Links
        External Data
        Linkouts
        KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
        Class of Gene
        Genomic Location and Detailed Mapping Data
        Chromosome (arm)
        3R
        Recombination map
        3-97
        Cytogenetic map
        98E5-98E5
        Sequence location
        FlyBase Computed Cytological Location
        Cytogenetic map
        Evidence for location
        98E5-98E5
        Limits computationally determined from genome sequence between P{PZ}l(3)0648706487 and P{EP}EP3390EP3390
        Experimentally Determined Cytological Location
        Cytogenetic map
        Notes
        References
        Experimentally Determined Recombination Data
        Location

        3-97

        (FlyBase, 2016)

        3-94.3

        (Comeron et al., 2012)
        Left of (cM)
        Right of (cM)
        Notes
        Stocks and Reagents
        Stocks (26)
        Genomic Clones (19)
         

        Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

        cDNA Clones (50)
         

        Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

        cDNA clones, fully sequenced
        BDGP DGC clones
        Other clones
        Drosophila Genomics Resource Center cDNA clones

        For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

        cDNA Clones, End Sequenced (ESTs)
        BDGP DGC clones
        Other clones
        RNAi and Array Information
        Linkouts
        DRSC - Results frm RNAi screens
        Antibody Information
        Laboratory Generated Antibodies
         

        monoclonal

        (Rodriguez-Mesa et al., 2012)

        polyclonal

        (Nahm et al., 2010, Ben-Yaacov et al., 2001)
        Commercially Available Antibodies
         
        Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
        Cell Line Information
        Publicly Available Cell Lines
         
          Other Stable Cell Lines
           
            Other Comments

            WASp is needed in wild-type cells for the death of Minute neighbours in cell competition experiments.

            (Li and Baker, 2007)

            The myoblast fusion phenotype in WASp mutants is based upon a specific defect in myoblast fusion not in cell fate determination.

            (Schafer et al., 2007)

            Hem antagonizes WASp function during myoblast fusion.

            (Schafer et al., 2007)

            dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

            (Kiger et al., 2003)

            dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.

            (Rogers et al., 2003)

            The cytoskeletal elements of WASp are required for it to perform its role in establishing lineage and cell shape.

            (Tal et al., 2002)

            WASp is required for cell fate decisions mediated by N signaling during adult and embryonic sensory organ development.

            (Ben-Yaacov et al., 2001)
            Relationship to Other Genes
            Source for database merge of

            Source for merge of: WASp CG1520

            (Goldstein and Gunawardena, 2000)
            Additional comments
            Nomenclature History
            Source for database identify of
            Nomenclature comments
            Etymology
            Synonyms and Secondary IDs (16)
            Reported As
            Symbol Synonym
            CG1520
            (Ibaraki et al., 2021, Hassan et al., 2018, Balakrishnan et al., 2015, Hallier et al., 2015, Hosono et al., 2015, Abmayr and Pavlath, 2012, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Perkins et al., 2009.1.15, Rogers et al., 2003, Goldstein and Gunawardena, 2000)
            D-WASP
            (Schejter et al., 2000, Schejter et al., 1999)
            Dm WASP
            (Renfranz and Beckerle, 2002)
            DmWASP
            (Linardopoulou et al., 2007)
            N-WASP
            (Fic et al., 2021)
            WASP
            (Fernandez-Gonzalez and Harris, 2023, Dubey et al., 2020, Kitatani et al., 2020, Popkova et al., 2020, Brüser and Bogdan, 2017, Transgenic RNAi Project members, 2017-, Martin, 2016, Koch et al., 2014, Levayer and Moreno, 2013, Ratnaparkhi, 2013, Duboff et al., 2012, Reed et al., 2012, Wang et al., 2012, Galy et al., 2011, Jin et al., 2011, Khuong et al., 2010, Serio et al., 2010, Desai et al., 2009, Berger et al., 2008, Schäfer et al., 2008, Chen, 2007, Gates et al., 2007, Kim et al., 2007, Kondylis et al., 2007, Schäfer et al., 2007, Schafer et al., 2005, Vartiainen and Machesky, 2004, Kunda et al., 2003, Rogers et al., 2003, Strunk et al., 2003, Unluer et al., 2003, Martin and Wood, 2002, Petit et al., 2002, Settleman, 2001, Goldstein and Gunawardena, 2000)
            WASp
            (Berry et al., 2024, Li et al., 2024, Pinot and Le Borgne, 2024, Hui et al., 2023, Hendricks et al., 2022, Papagiannouli, 2022, Rout et al., 2022, Rujano et al., 2022, Dondi et al., 2021, Ibaraki et al., 2021, Klann et al., 2021, Loganathan et al., 2021, Sahu et al., 2021, Stark et al., 2021, Yin et al., 2021, Deng et al., 2020, Lin et al., 2020, Gao et al., 2019, Kim et al., 2019, Trylinski and Schweisguth, 2019, Yang and Chien, 2019, Hassan et al., 2018, Lee et al., 2018, Salazar and Yamamoto, 2018, Transgenic RNAi Project members, 2017-, Bothe and Baylies, 2016, Dubey et al., 2016, Hamp et al., 2016, Sarov et al., 2016, Segal et al., 2016, Stanishneva-Konovalova et al., 2016, Gene Disruption Project members, 2015-, Kamiyama et al., 2015, Rodal et al., 2015, Uytterhoeven et al., 2015, Abreu-Blanco et al., 2014, Bothe et al., 2014, Han et al., 2014, Haralalka et al., 2014, Merlo et al., 2014, Sharifai et al., 2014, Kaipa et al., 2013, Parkhurst, 2013.10.2, Abmayr and Pavlath, 2012, Förster and Luschnig, 2012, Japanese National Institute of Genetics, 2012.5.21, Rodriguez-Mesa et al., 2012, Benhra et al., 2011, Cernilogar et al., 2011, Friedman et al., 2011, Mosca and Schwarz, 2010, Gildor et al., 2009, Rajan et al., 2009, Johnson et al., 2008, Magalhaes et al., 2007, Schejter et al., 2007, Schejter, 2006.7.3, Biyasheva et al., 2004)
            WSP
            (Khuong et al., 2010)
            Wasp
            (Pradhan et al., 2023, Xie et al., 2021, Hicks et al., 2015, Rosa et al., 2015, Zobel et al., 2015, Hombría and Sotillos, 2013, Fabian and Brill, 2012, Gault et al., 2012, Rojas-Ríos et al., 2012, Sens et al., 2010, Liu et al., 2009, Cavey et al., 2008, Erfurth and Schmucker, 2008, Besse et al., 2007, Le Borgne et al., 2005, Polesello and Payre, 2004, Jacinto and Baum, 2003, Hudson and Cooley, 2002, Zallen et al., 2002, Schejter et al., 2001, Worby et al., 2001, Zallen et al., 2001)
            Wsp
            (Zoller and Schulz, 2012, Mukherjee et al., 2011, Rotkopf et al., 2011, Zhu and Bhat, 2011, Mosca and Schwarz, 2010, Sens et al., 2010, Gildor et al., 2009, Kumar et al., 2009, Kumar et al., 2009, Cavey et al., 2008, Rodal et al., 2008, Massarwa et al., 2007, Collins and DiAntonio, 2004, Coyle et al., 2004, Ng and Luo, 2004, Tal et al., 2002, Zallen et al., 2002, Ben-Yaacov et al., 2001)
            dWASP
            (Burbelo, 2001.7.27, Pirone et al., 2001)
            wasp
            (Bourouliti and Skoulakis, 2022, Molina-Pelayo et al., 2022, Zhang et al., 2020, Lee and Chen, 2019, Brinkmann et al., 2016, Hamp et al., 2016, Hallier et al., 2015, Rudolf et al., 2014, Kaipa et al., 2013, Külshammer and Uhlirova, 2013, Duan et al., 2012, Koch et al., 2012, Levayer et al., 2011, Stephan et al., 2011, Eriksson et al., 2010, Georgiou and Baum, 2010, Bertet et al., 2009, Fricke et al., 2009, Berger et al., 2008, Georgiou et al., 2008, Leibfried et al., 2008, Schäfer et al., 2008, Stephan et al., 2008, Li and Baker, 2007, Li and Baker, 2007, Schafer et al., 2007, Kochubey et al., 2006, Bogdan et al., 2005, Stephan et al., 2005, Bogdan et al., 2004, Broadie, 2004, Luschnig et al., 2004, Bogdan and Klambt, 2003)
            wsp
            (Hossain et al., 2023, Krämer et al., 2023, Brinkmann et al., 2016, Deng et al., 2015, Giuliani et al., 2013, Gault et al., 2012, Kim et al., 2011, Khuong et al., 2010, Khuong et al., 2010, Nahm et al., 2010, Nahm et al., 2010, Sens et al., 2010, Warner and Longmore, 2009, Corrigall et al., 2007, Chanut-Delalande et al., 2006, Frank et al., 2006, Ackley and Jin, 2004)
            Name Synonyms
            WASP
            (Rogers et al., 2003)
            WASp
            (Kumar et al., 2009)
            Wasp
            (Corrigall et al., 2007)
            Wiscott-Aldrich syndrome protein
            (Khuong et al., 2010)
            Wiskott-Aldrich Syndrome protein
            (Dubey et al., 2016)
            Wiskott-Aldrich syndrome protein
            (Amoyel and Bach, 2014, Stephan et al., 2011, Gildor et al., 2009, Erfurth and Schmucker, 2008, Johnson et al., 2008)
            wasp
            (Chanut-Delalande et al., 2006)
            Secondary FlyBase IDs
            • FBgn0039603
            Datasets (0)
            Study focus (0)
            Experimental Role
            Project
            Project Type
            Title
            Study result (0)
            Result
            Result Type
            Title
            External Crossreferences and Linkouts ( 59 )
            Sequence Crossreferences
            NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
            GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
            GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
            RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
            UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
            UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
            Other crossreferences
            AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
            BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
            DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
            EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
            FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
            FlyMine - An integrated database for Drosophila genomics
            InterPro - A database of protein families, domains and functional sites
            KEGG Genes - Molecular building blocks of life in the genomic space.
            MARRVEL_MODEL - MARRVEL (model organism gene)
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
            DroID - A comprehensive database of gene and protein interactions.
            DRSC - Results frm RNAi screens
            Developmental Studies Hybridoma Bank - Monoclonal antibodies for use in research
            Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
            FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
            FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
            Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
            Flygut - An atlas of the Drosophila adult midgut
            iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
            Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
            KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.
            MIST (genetic) - An integrated Molecular Interaction Database
            MIST (protein-protein) - An integrated Molecular Interaction Database
            References (254)