HomeGene Dmel\trio
| General Information | |||
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| Symbol | Dmel\trio | Species | D. melanogaster |
| Name | trio | Annotation symbol | CG18214 |
| Feature type | protein_coding_gene | FlyBase ID | FBgn0024277 |
| Created / Updated | 2003-12-01/2003-12-01 | ||
| Genomic Location | |||
| Chromosome (arm) | 3L | Recombination map | 3- |
| Cytogenetic map | 61E1-61E2 | Sequence location | 3L:995,978..1,034,875 [-] |
| Map ( GBrowse ) | 
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Summary Information
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Automatically generated summary
See sections below for more information | This gene is referred to in FlyBase by the symbol trio (CG18214, FBgn0024277). It has the cytological map location 61E1-61E2. Its sequence location is 3L:995978..1034875. Its molecular function is described as Rho guanyl-nucleotide exchange factor activity. It is involved in the biological processes: axon guidance; central nervous system development; peripheral nervous system development; actin cytoskeleton organization and biogenesis; Rho protein signal transduction; regulation of axonogenesis; regulation of Rho protein signal transduction. 75 alleles are reported. The phenotypes of these alleles are annotated with 19 unique terms, many of which group under: nervous system; organ system; adult segment; embryonic nervous system; adult brain; commissure; peripheral nervous system; larval nervous system; embryonic neuron; embryonic somatic muscle; adult mesothoracic segment. It has 6 annotated transcripts and 6 annotated polypeptides. | ||
| External Summaries | |||
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Detailed Mapping Data
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| FlyBase Computed Cytological Location | |||
Cytogenetic map Evidence for location 61E1-61E2
Limits computationally determined from genome sequence between P{PZ}l(3)0596705967 and P{PZ}l(3)0264002640
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| Experimentally Determined Cytological Location | |||
Cytogenetic map Notes References 61E1-61F2 61E-61E 61F8-61F8 (determined by in situ hybridisation) 61E (determined by in situ hybridisation)
61E-61E (determined by in situ hybridisation) 61F8 (determined by in situ hybridisation) 61F1--2 (determined by in situ hybridisation)
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| Experimentally Determined Recombination Data | |||
| Location | |||
| Left of (cM) | |||
| Right of (cM) | |||
| Notes | |||
| Molecular Map Data | |||
Gene Order (in direction of increasing cytology)
References Gene Order (overall orientation not stated) References | |||
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Gene Model & Products
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Please see the
GBrowse view of
Dmel\trio
for information on other features
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| Comments on Gene Model | |||
DGC clone GH01987 appears problematic: incomplete CDS. DGC does not match predicted CDS | |||
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Transcript Data
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| Annotated Transcripts | |||
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) | |||
| Additional Transcript Data & Comments | |||
| Reported size (kB) | 8.0 (northern blot) | ||
| Comments | |||
| External Data | |||
| Crossreferences | |||
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Polypeptide Data
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| Annotated Polypeptides | |||
Name FlyBase ID
Predicted MW (kD)
Length (aa)
Theoretical pI
RefSeq ID
GenBank protein
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| Additional Polypeptide Data & Comments | |||
| Reported size (kD) | 2263 (aa) | ||
| Comments | |||
| External Data | |||
| Linkouts | PANTHER
- Protein classification by function, families, and pathways
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| Crossreferences | InterPro
domains - A database of protein families, domains, and functional sites
• Dbl homology (DH) domain (IPR000219)
Src homology-3 (IPR001452)
Pleckstrin-like (IPR001849)
Spectrin repeat (IPR002017)
Pleckstrin homology-type (IPR011993)
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Sequences Consistent with the Gene Model
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| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
| UniProtKB/TrEMBL | |||
| Maps to | |||
| Does NOT map to | |||
| Identified with | |||
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Mapped Features & Mutations
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Please see
GBrowse
or insertion reports for information on insertions of transgenic
constructs and features not listed here
Type Symbol & Location Additional Notes References point mutation trio[E4.1] 3L:1,028,454..1,028,454 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C1028454T pr_change=Q261|trio-PA,Q261|trio-PC reported_pr_change=Q261@ point mutation trio[1] 3L:1,028,290..1,028,291 comment=G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental pr_change=W315|trio-PC,W315|trio-PA reported_pr_change=W315@ point mutation trio[8] 3L:1,027,398..1,027,398 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C1027398T pr_change=Q613|trio-PA,Q613|trio-PC reported_pr_change=Q613@ point mutation trio[4] 3L:1,026,849..1,026,849 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C1026849T pr_change=Q796|trio-PA,Q796|trio-PC point mutation trio[M89] 3L:1,024,942..1,024,942 evidence=experimental na_change=C1024942T pr_change=L1412F|trio-PA,L1412F|trio-PC reported_na_change=C?T reported_pr_change=L1412F | |||
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External Data
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| Linkouts | DEDB
- Drosophila exon database: splicing graphs
• | ||
| Crossreferences | |||
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Expression Data
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| FlyBase-Curated Data | |||
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Transcript and Protein data | Please see the FlyBase Gene Expression Report for details of gene expression from the literature. | ||
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Summary of Transcript Expression
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Stage Tissue/Position
Reference
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| Marker for | |||
| Subcellular Localization | |||
| CV Term | |||
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Summary of Polypeptide Expression
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Stage Tissue/Position
Reference
adult stage
mushroom body
adult stage
lamina
larval stage | third instar
axon
larval stage,pupal stage
mushroom body
larval stage,adult stage
calyx of mushroom body
larval stage | third instar
eye imaginal disc
adult stage
adult brain
embryonic stage
epidermis
adult stage
neuron
embryonic stage
longitudinal connectives
larval stage,adult stage
γ-lobe
adult stage
α\'-lobe
embryonic stage | stage >=12
axon
adult stage
β\'-lobe
adult stage
ellipsoid body
larval stage | third instar
growth cone
embryonic stage
muscle attachment site
embryonic stage
commissure | |||
| Marker for | |||
| Subcellular Localization | |||
| CV Term | plasma membrane cytoplasm | ||
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Microarray Data
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Developmental timecourse, Costello et al., 2008 (Original data from Arbeitman et al., 2002) 
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External Data & Images
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| Linkouts | FLIGHT
- Cell culture data for RNAi and other high-throughput technologies
FlyAtlas
- Adult expression by tissue, using Affymetrix Dros2 array
GEO (NCBI)
- Gene expression data: microarray and other high-throughput technologies
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Alleles & Phenotypes
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Summary of Allele Phenotypes
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Lethality Allele viable (with trioS036810) lethal (with Df(3L)Fpa1) semi-lethal (with Df(3L)Fpa1) Other Phenotypes Allele visible (with trioS036810) Sterility Allele Phenotype manifest in Allele photoreceptor cell & axon (with trioS138606) | |||
