DTrio, l(3)S036810, l(3)S095914, M89
Rac guanyl-nucleotide exchange factor - Loss-of-function mutations result in the misdirection or stall of axons in embryos and also cause malformation of the mushroom body - functions in sculpting class specific dendrite morphogenesis in Drosophila sensory neurons
Low-frequency RNA-Seq exon junction(s) not annotated.
Gene model reviewed during 5.46
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 6.02
8.0 (northern blot)
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\trio using the Feature Mapper tool.
trio protein is distributed in a patched pattern in the axons and cell bodies. The patches are in many cases associated with clusters of vesicles in the axoplasm. In the lamina, trio protein is found in the dendritic terminals that contact or intrude into the photoreceptor cells.
trio protein is detected in embryos along axon fascicles starting in stage 12. By stage 16 it is detected primarily in longitudinal connectives and weakly in commissures of the CNS. trio protein is also detected in the epidermis and in muscle attachment sites. trio protein is detected throughout development. In the adult brain, weak staining is seen in a large number of cells in the cortex and in most neuropil regions. Strong staining is observed in mushroom body, lamina, and ellipsoid body neurons. Within the mushroom body, staining is observed in the alpha\', beta\' and gamma lobes but not in the alpha and beta lobes. trio protein is also observed in the developing mushroom body during larval and pupal stages.
trio protein is expressed in third instar eye imaginal discs in photoreceptor cells as well as in undifferentiated and nonneuronal cells. It is observed along the entire length of the axons as well as in growth cones in dissociated eye disc cells allowed to differentiate in vitro.
GBrowse - Visual display of RNA-Seq signalsView Dmel\trio in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of Trio CG9208 was sequence comparison ( date:000525 ).
dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.
dsRNA made from templates generated with primers directed against this gene is tested in an RNAi screen for effects on actin-based lamella formation.
trio is essential in order for neurites to faithfully extend on the correct pathways. It may also play a postdevelopmental role in neurite terminals.
Mutants deficient in trio activity display defects in both central and peripheral axon pathways reminiscent of phenotypes observed in embryos deficient in small GTPase function.
Isolated on the basis of sequence similarity to human Trio.
Haploinsufficiency dependent upon an Abl mutant background (HDA).