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Gene Dmel\Sir2

General Information
Symbol	Dmel\Sir2	Species	D. melanogaster
Name	Sir2	Annotation symbol	CG5216
Feature type	protein_coding_gene	FlyBase ID	FBgn0024291
Gene Model Status	Current	Stock availability	18 publicly available
Also Known As	dSir2
Genomic Location
Chromosome (arm)	2L	Recombination map
Cytogenetic map	34A7-34A7	Sequence location	2L:13,165,564..13,169,551 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Sir2 is referred to in FlyBase by the symbol Dmel\Sir2 (CG5216, FBgn0024291). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: transcription factor binding; NAD-dependent histone deacetylase activity; histone deacetylase activity. There is experimental evidence that it is involved in the biological process: regulation of apoptotic process; histone deacetylation; regulation of transcription, DNA-dependent; determination of adult lifespan; regulation of histone acetylation; behavioral response to ethanol; chromatin silencing; response to nutrient. 31 alleles are reported. The phenotypes of these alleles are annotated with: nucleolus; chromatin. It has one annotated transcript and one annotated polypeptide. Protein features are: Sirtuin family; Sirtuin family, catalytic core domain; Sirtuin family, catalytic core small domain. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult brain, larval/adult salivary gland, adult female reproductive system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval trachea, inseminated spermatheca, adult male accessory gland, larval/adult carcass. Comments on Affy2 ProbeSet: ProbeSet 1628368_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Sir2. Gene sequence location is 2L:13165564..13169551.
User Contributed Data
	Beta version, feedback welcome
External Summaries
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 34A7-34A7   Limits computationally determined from genome sequence between P{lacW}l(2)k11328k11328&P{lacW}l(2)k10105k10105 and P{EP}EP850&P{lacW}l(2)k05911k05911
Experimentally Determined Cytological Location
Cytogenetic map Notes References 34A-34A   (determined by in situ hybridisation)   (Astrom et al., 2003) 34A5-34A6   (determined by in situ hybridisation)   (Newman et al., 2002) 34A5-34A6     (Astrom, 1998.5.27) 34A5-34A6   (determined by in situ hybridisation)   (Spradling et al., 1999) 34A-34A   (determined by in situ hybridisation)   (Smolik, 1998.3.10) 34A5-34A6     (BDGP Project Members, 1994-1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Sir2 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.44 Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Sir2-RA FBtr0080434  NM_058003   3809   823
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Sir2-PA   FBpp0080015   91.8   823   4.48     NP_477351   AAF53248
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Sirtuin family (IPR003000) Sirtuin family, catalytic core small domain (IPR026591) Sirtuin family, catalytic core domain (IPR026590)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA202443   AA264032   AA438971   AA439355   AA735912   AA949863   AE014134 AAF53248   AC010214   AF068758 AAC79684   AI405626   AI519056   AI542558   AI542563   AI945051   AQ073472   AW941639   AZ300944   BF487547   BF487564   BG635180   BG637565   BG638040   BG638677   BG639937   BI172648   BI229770   BI235387   BI366380   BI486551   BI567490   BI570872   BI571306   BI571340   BI571637   BI571919   BI572311   BI572558   BI572855   BI579068   BI582135   BI582431   BI583098   BI586245   BI591331   BI591882   BI629772   BI633939   BI635798   BT004901 AAO47879   BT120355   BT124857   BT133215   BT133274   CO177187   CO177917   CO178583   CO185514   CO186091   CO188061   CO192459   CO262805   CO271434   CO277287   CO280033   CO281748   CO282049   CO283022   CO284356   CO285265   CO286882   CO288428   CO295521   CO298197   CO301984   CO308493   CO312982   CO316248   CO323845   CO329446   DN154401   EC069607   EC069797   EC070645   EC077984   EC083182   EC084975   EC085254   EC087341   EC087974   EC194487   EC200773   EC207456   EC211200   EC214236   EC229655   EC232103   EC236007   EC239326   EC239370   EC239415   EC241887   EC242564   EC245139   EC245663   EC245895   EC248810   EC249804   EC253044   EV581112   EV595945   EV598894   GH749557   GH752427   GH752938   GH771706   GH772474   GH778772   GH780369   GH781570   GH783453   GH794449   GH794923   GH799893   GH803918   GH805048   GH808457   GH808541   GH808979   GH813888   GH813921   GH815378   GH816487   GH816638   GH818575   GH818698   GH819040   GH821686   GH823811   GH837409   GH848246   GH849476   GH849683   GH850270   GH857325   GH858620   GH870919   GH872219   GH874967   GH875267   GH875583   GH876966   GH883902   GH883965   GH886807   GH888509   GH888574   GH890372   GH894586   GH896406   GH896967   GH897137   GH897164   GH899946   GH901844   GH902122   GH904565   GH912047   GH913120   GH915378   GH917246   GH920759   GH922060   GH922705   GH923252   GH923628   GH929930   GH936780   GH937334   GH940211
UniProtKB/Swiss-Prot	Q9VK34
UniProtKB/TrEMBL	Q86LE9
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism | ubiquitous   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 11 -- 12   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 13 -- 16   organism | ubiquitous   (Fisher et al., 2012)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Newman et al., 2002) nucleoplasm inferred from direct assay (Tulin et al., 2006) nucleus inferred from direct assay (Newman et al., 2002, Cho et al., 2005)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Many larval and adult organs/tissues expressed at moderate levels. Expression at high levels in the following post-embryonic organs or tissues: adult brain, larval/adult salivary gland, adult female reproductive system. Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval/adult midgut, larval/adult hindgut, larval/adult Malpighian tubules, adult heart, larval/adult fat body, larval trachea, inseminated spermatheca, adult male accessory gland, larval/adult carcass. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland    511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain    553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland    616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca    508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary    758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland  511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain  (553.9) Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland  (616.2) Adult Heart    356.95 Adult VirginFemale Spermatheca  508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary  (758.8) Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland    511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain    553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland    616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca    508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary    758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland    511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain    553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland    616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca    508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary    758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland    511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain    553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland    616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca    508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary    758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland  511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain  553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland  616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca  508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary  758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland    511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain    553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland    616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca    508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary    758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    471.8 Larval Midgut    258.7 Larval Hindgut    328 Larval Malpighian Tubules    313.5 Larval Fat Body    132.5 Larval Salivary Gland    511.9 Larval Trachea    328 Larval Carcass    319.95 Adult Head    384.5 Adult Eye    373.825 Adult Brain    553.9 Adult Thoracic-Abdominal Ganglion    398.3 Adult Crop    378.4 Adult Midgut    296.8 Adult Hindgut    420.1 Adult Malpighian Tubules    373.8 Adult Fat Body    220.7 Adult Salivary Gland    616.2 Adult Heart    356.95 Adult VirginFemale Spermatheca    508.4 Adult InseminatedFemale Spermatheca    476.4 Adult Ovary    758.8 Adult Testis    32.1 Adult Male Accessory Gland    309.8 Adult Carcass    194.9 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    384.5   NA  Eye    373.825   NA  Brain    553.9   471.8  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    398.3   NA  Crop    378.4   258.7  Midgut    296.8   328  Hindgut    420.1   313.5  Malpighian Tubules    373.8   132.5  Fat Body    220.7   511.9  Salivary Gland    616.2   NA  Heart    356.95   328  Trachea    NA   NA  VirginFemale Spermatheca    508.4   NA  InseminatedFemale Spermatheca    476.4   NA  Ovary    758.8   NA  Testis    32.1   NA  Male Accessory Gland    309.8   319.95  Carcass    194.9 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    33 central nervous system, pupae P8    30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male    30 head, mated 20-day male    29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering    28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    71 ovary, mated 4-day female    71 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3  (33) central nervous system, pupae P8  (30) head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male  (30) head, mated 20-day male  (29) salivary gland, larvae L3 wandering    11 salivary gland, white prepupae  (31) digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering  (28) carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female  (71) ovary, mated 4-day female  (71) testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    33 central nervous system, pupae P8    30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male    30 head, mated 20-day male    29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering    28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    71 ovary, mated 4-day female    71 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    33 central nervous system, pupae P8    30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male    30 head, mated 20-day male    29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering    28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    71 ovary, mated 4-day female    71 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    33 central nervous system, pupae P8    30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male    30 head, mated 20-day male    29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering    28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    71 ovary, mated 4-day female    71 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3  (33) central nervous system, pupae P8  30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male  30 head, mated 20-day male  29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae  31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering  28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female  (71) ovary, mated 4-day female  (71) testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    33 central nervous system, pupae P8    30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male    30 head, mated 20-day male    29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering    28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    71 ovary, mated 4-day female    71 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    20 central nervous system, larvae L3    33 central nervous system, pupae P8    30 head, virgin 1-day female    16 head, virgin 4-day female    20 head, virgin 20-day female    15 head, mated 1-day female    18 head, mated 4-day female    19 head, mated 20-day female    15 head, mated 1-day male    24 head, mated 4-day male    30 head, mated 20-day male    29 salivary gland, larvae L3 wandering    11 salivary gland, white prepupae    31 digestive system, larvae L3 wandering    18 digestive system, 1-day adult    15 digestive system, 4-day adult    20 digestive system, 20-day adult    17 fat body, larvae L3 wandering    6 fat body, white prepupae    10 fat body, pupae P8    16 carcass, larvae L3 wandering    28 carcass, 1-day adult    11 carcass, 4-day adult    16 carcass, 20-day adult    12 ovary, virgin 4-day female    71 ovary, mated 4-day female    71 testis, mated 4-day male    5 accessory gland, mated 4-day male    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of moderate expression. Peak expression observed within 00-12 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    53 embryo 04-06hr    62 embryo 06-08hr    55 embryo 08-10hr    47 embryo 10-12hr    33 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    37 embryo 18-20hr    30 embryo 20-22hr    39 embryo 22-24hr    44 larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day    41 adult female 30day    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (77) embryo 02-04hr  (53) embryo 04-06hr  (62) embryo 06-08hr  (55) embryo 08-10hr  (47) embryo 10-12hr  (33) embryo 12-14hr  (39) embryo 14-16hr  (37) embryo 16-18hr  (37) embryo 18-20hr  (30) embryo 20-22hr  (39) embryo 22-24hr  (44) larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day  (41) adult female 30day  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    53 embryo 04-06hr    62 embryo 06-08hr    55 embryo 08-10hr    47 embryo 10-12hr    33 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    37 embryo 18-20hr    30 embryo 20-22hr    39 embryo 22-24hr    44 larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day    41 adult female 30day    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    53 embryo 04-06hr    62 embryo 06-08hr    55 embryo 08-10hr    47 embryo 10-12hr    33 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    37 embryo 18-20hr    30 embryo 20-22hr    39 embryo 22-24hr    44 larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day    41 adult female 30day    40 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    53 embryo 04-06hr    62 embryo 06-08hr    55 embryo 08-10hr    47 embryo 10-12hr    33 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    37 embryo 18-20hr    30 embryo 20-22hr    39 embryo 22-24hr    44 larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day    41 adult female 30day    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (77) embryo 02-04hr  (53) embryo 04-06hr  (62) embryo 06-08hr  (55) embryo 08-10hr  (47) embryo 10-12hr  (33) embryo 12-14hr  (39) embryo 14-16hr  (37) embryo 16-18hr  (37) embryo 18-20hr  30 embryo 20-22hr  (39) embryo 22-24hr  (44) larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day  (41) adult female 30day  (40) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    53 embryo 04-06hr    62 embryo 06-08hr    55 embryo 08-10hr    47 embryo 10-12hr    33 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    37 embryo 18-20hr    30 embryo 20-22hr    39 embryo 22-24hr    44 larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day    41 adult female 30day    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    77 embryo 02-04hr    53 embryo 04-06hr    62 embryo 06-08hr    55 embryo 08-10hr    47 embryo 10-12hr    33 embryo 12-14hr    39 embryo 14-16hr    37 embryo 16-18hr    37 embryo 18-20hr    30 embryo 20-22hr    39 embryo 22-24hr    44 larva L1    17 larva L2    13 larva L3 12hr old    9 larva L3 puffstage 1-2    10 larva L3 puffstage 3-6    17 larva L3 puffstage 7-9    21 white prepupae new    24 white prepupae 12hr    24 white prepupae 24hr    25 pupae 2d postWPP    25 pupae 3d postWPP    14 pupae 4d postWPP    14 adult male 01day    14 adult male 05day    15 adult male 30day    13 adult female 01day    23 adult female 05day    41 adult female 30day    40 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    20 embryonic 1182-4H    29 embryonic GM2    20 embryonic Kc167    52 embryonic S1    32 embryonic S3    32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    17 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    34 CNS ML-DmBG2-c2    45 tumorous blood cells mbn2    33 ovary fGS/OSS    124 ovary OSC    30 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4    20 embryonic 1182-4H  (29) embryonic GM2    20 embryonic Kc167  (52) embryonic S1  (32) embryonic S3  (32) leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8  (30) wing disc ML-DmD9  26 wing disc ML-DmD16-c3  (50) wing disc ML-DmD21    17 wing disc ML-DmD32  (29) haltere disc ML-DmD17-c3  (63) eye-antennal disc ML-DmD11  (34) antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1  (34) CNS ML-DmBG2-c2  (45) tumorous blood cells mbn2  (33) ovary fGS/OSS  (124) ovary OSC  (30) ovary OSS  (30) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    20 embryonic 1182-4H    29 embryonic GM2    20 embryonic Kc167    52 embryonic S1    32 embryonic S3    32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    17 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    34 CNS ML-DmBG2-c2    45 tumorous blood cells mbn2    33 ovary fGS/OSS  (124) ovary OSC    30 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    20 embryonic 1182-4H    29 embryonic GM2    20 embryonic Kc167    52 embryonic S1    32 embryonic S3    32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    17 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    34 CNS ML-DmBG2-c2    45 tumorous blood cells mbn2    33 ovary fGS/OSS    124 ovary OSC    30 ovary OSS    30 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    20 embryonic 1182-4H    29 embryonic GM2    20 embryonic Kc167    52 embryonic S1    32 embryonic S3    32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    17 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    34 CNS ML-DmBG2-c2    45 tumorous blood cells mbn2    33 ovary fGS/OSS    124 ovary OSC    30 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (36) Schneider line 2 Sg4    20 embryonic 1182-4H  29 embryonic GM2    20 embryonic Kc167  (52) embryonic S1  32 embryonic S3  32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8  30 wing disc ML-DmD9  26 wing disc ML-DmD16-c3  (50) wing disc ML-DmD21    17 wing disc ML-DmD32  29 haltere disc ML-DmD17-c3  (63) eye-antennal disc ML-DmD11  (34) antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1  (34) CNS ML-DmBG2-c2  (45) tumorous blood cells mbn2  (33) ovary fGS/OSS  (124) ovary OSC  30 ovary OSS  30 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    20 embryonic 1182-4H    29 embryonic GM2    20 embryonic Kc167    52 embryonic S1    32 embryonic S3    32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    17 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    34 CNS ML-DmBG2-c2    45 tumorous blood cells mbn2    33 ovary fGS/OSS  124 ovary OSC    30 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    36 Schneider line 2 Sg4    20 embryonic 1182-4H    29 embryonic GM2    20 embryonic Kc167    52 embryonic S1    32 embryonic S3    32 leg disc CME L1    23 wing disc CME-W2    18 wing disc ML-DmD8    30 wing disc ML-DmD9    26 wing disc ML-DmD16-c3    50 wing disc ML-DmD21    17 wing disc ML-DmD32    29 haltere disc ML-DmD17-c3    63 eye-antennal disc ML-DmD11    34 antennal disc ML-DmD20-c5    22 mixed discs ML-DmD4-c1    22 CNS ML-DmBG1-c1    34 CNS ML-DmBG2-c2    45 tumorous blood cells mbn2    33 ovary fGS/OSS    124 ovary OSC    30 ovary OSS    30 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    42 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    62 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult  (38) Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult  (27) Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (42) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  (62) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (49) Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult  (45) Paraquat 10 mM 48 hrs, 4-day adult  (43) Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    42 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    62 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    42 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    62 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    42 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    62 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult  (38) Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult  27 Cadmium 100 mM 48 hrs, 4-day adult  (67) Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult  (42) Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3  (62) Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult  (49) Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult  (45) Paraquat 10 mM 48 hrs, 4-day adult  (43) Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    42 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    62 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    24 cold shock, 4-day adult    17 heat shock, 4-day adult    38 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    17 Cadmium 50 mM 48 hrs, 4-day adult    27 Cadmium 100 mM 48 hrs, 4-day adult    67 Copper 0.5 mM 12 hrs, larvae L3    16 Copper 15 mM 48 hrs, 4-day adult    42 Zinc 5 mM 12 hrs, larvae L3    13 Zinc 4.5 mM 48 hrs, 4-day adult    18 Ethanol 2.5% 3 hrs, larvae L3    62 Ethanol 5% 3 hrs, larvae L3    13 Ethanol 10% 3 hrs, larvae L3    12 Caffeine 1.5 mg/ml 4 hrs, larvae L3    13 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    49 Caffeine 25 mg/ml 48 hrs, 4-day adult    23 Paraquat 5 mM 48 hrs, 4-day adult    45 Paraquat 10 mM 48 hrs, 4-day adult    43 Rotenone 2 μg 12 hrs, larvae L3    12 Rotenone 8 μg 12 hrs, larvae L3    9 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_03
External Data & Images
Linkouts	BDGP expression data • LD07439 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024291 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5216-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal | embryonic stage, with Scer\GAL4Act5C.PU Sir2dsRNA.Scer\UAS.cKa partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 Sir2GD11580 viable Sir22A-7-11 Sir2CB02821 viable, with Scer\GAL4[-]: Sir2EP2300 viable (with Sir24.5) Sir25.26 viable (with Sir25.26) Sir24.5 viable | female Sir217 viable | female (with Sir217) Sir2ex10 viable | female (with Sir2ex10) Sir217 viable | male Sir217 viable | male (with Sir217) Sir2ex10 viable | male (with Sir2ex10) Sir217 Sterility Allele fertile Sir22A-7-11 fertile (with Sir24.5) Sir25.26 fertile (with Sir25.26) Sir24.5 Other Phenotypes Allele chemical resistant | conditional Sir22A-7-11 Sir2NP1145 chemical resistant | conditional, with Sir22A-7-11 Sir2NP1145 chemical resistant | conditional, with Sir2NP1145 Sir22A-7-11 enhancer of variegation, with Scer\GAL4da.G32 Sir2Scer\UAS.T:Disc\RFP hypoactive | conditional Sir22A-7-11 Sir2NP1145 hypoactive | conditional, with Sir22A-7-11 Sir2NP1145 hypoactive | conditional, with Sir2NP1145 Sir22A-7-11 long lived, with Scer\GAL4arm.PS Sir2EP2300 long lived, with Scer\GAL4elav-C155 Sir2EP2300 long lived, with Scer\GAL4αTub84B.PL Sir2EP2300 Sir2EP2384 long lived | male limited, with Scer\GAL4αTub84B.PL Sir2EY03602 long lived | RU486 conditional, with Scer\GAL4elav.Switch.PO Sir2EP2300 non-suppressor of variegation, with P{hsp26-pt-T}39C-5 Sir2ex10 non-suppressor of variegation, with P{hsp26-pt-T}39C-55 Sir2ex10 short lived Sir244 short lived, with Scer\GAL4elav.PU Sir2dsRNA.Scer\UAS.cKa short lived | female limited, with Scer\GAL4αTub84B.PL Sir2EY03602 suppressor of variegation, with In(1)wm4 Sir217 suppressor of variegation (with Sir24.5), with In(1)wm4 Sir25.26 suppressor of variegation (with Sir25.26), with In(1)wm4 Sir24.5 suppressor of variegation | dominant, with In(1)wm4 Sir2ex10 suppressor of variegation | dominant, with P{hsp26-pt-T}39C-72 Sir2ex10 suppressor of variegation | dominant, with P{lacW}50C.x6 Sir2ex10 Phenotype manifest in Allele chromatin, with Scer\GAL4da.G32 Sir2Scer\UAS.T:Disc\RFP nucleolus, with Scer\GAL4da.G32 Sir2Scer\UAS.T:Disc\RFP
Classical Alleles ( 18 )
For All Classical Alleles Show
Allele of Sir2 Class Mutagen Stocks Known lesion Sir22A-7-11 amorphic allele - molecular evidence recombination I-CreI endonuclease ends-in gene targeting 1 Yes Sir205327a P-element activity 1 Yes Sir217 Delta2-3 1 Yes Sir24.5 1 Yes Sir25.26 1 Yes Sir2EP2300 P-element activity 1 Yes Sir2EP2384 P-element activity 1 Yes Sir2EY22867 P-element activity 1 -- Sir2KG00871 1 -- Sir2NP1145 1 -- Sir2NP2557 1 -- Sir2NP4739 1 -- Sir244 0 -- Sir2CB02821 P-element activity 0 Yes Sir2EP2143 P-element activity 0 Yes Sir2EP2436 P-element activity 0 Yes Sir2ex10 P-element activity 0 Yes Sir2EY03602 0 --
Alleles Carried on Transgenic Constructs ( 13 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Sir2 Class Mutagen Stocks Known lesion Sir2GD11580 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Sir2HMS00484 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sir2JF01423 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sir2KK107325 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sir2KK109370 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Sir2Act5C.T:SV5\V5,T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Sir2Act5C.T:Zzzz\His6 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Sir2dsRNA.Scer\UAS.cKa in vitro construct - regulatory fusion in vitro construct - RNAi 0 Yes Sir2hs.C.T:Ecol\dam,T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Sir2NIG.5216R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Sir2Scer\SceI.RS.cXa in vitro construct 0 Yes Sir2Scer\UAS.P\T.T:Hsap\MYC in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes Sir2Scer\UAS.T:Disc\RFP in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Not disrupted in	Df(2L)Prl (Spradling et al., 1999, BDGP Project Members, 1994-1999)
Disrupted in	Df(2L)Exel8029 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD11580} NA P{KK107325} NA P{KK109370} NA P{NIG.5216R} NA P{TRiP.HMS00484} NA P{TRiP.JF01423} NA P{UASp-Sir2.Myc} NA P{UAS-Sir2.IR} NA P{UAS-Sir2-DsRed} NA characterization construct P{Sir2Scer\SceI.RS.cXa} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EP}DnaJ-HEP2143 NA P{EP}DnaJ-HEP2436 NA P{EP}Sir2EP2300 NA P{EP}Sir2EP2384 NA P{EPgy2}Sir2EY03602 NA P{EPgy2}Sir2EY22867 NA P{Mae-UAS.6.11}UY126UY126 NA insertion of enhancer trap binary system P{GawB}Sir2NP1145 No P{GawB}Sir2NP2557 No P{GawB}Sir2NP4739 No miscellaneous insertions P{?PZ}Sir2ex10 NA P{PTT-GB}DnaJ-HCB02821 NA P{SUPor-P}Sir2KG00871 NA insertion of enhancer trap P{lacW}k06008a No P{lacW}k14513 No P{PZ}Sir205327a No
Gene Ontology: Function, Process & Cellular Component ( 15 unique terms )
Terms Based on Experimental Evidence ( 14 terms )
Molecular Function
CV term Evidence References
histone deacetylase activity inferred from direct assay (Barlow et al., 2001, Cho et al., 2005) NAD-dependent histone deacetylase activity inferred from direct assay (Borra et al., 2002, Rosenberg and Parkhurst, 2002, Newman et al., 2002, Furuyama et al., 2004) transcription factor binding inferred from physical interaction with nej (Smolik, 2009)
Biological Process
CV term Evidence References
behavioral response to ethanol inferred from mutant phenotype (Kong et al., 2010) chromatin silencing inferred from mutant phenotype (Tulin et al., 2006) determination of adult lifespan inferred from mutant phenotype (Kapahi, 2003, Kusama et al., 2006) NOT determination of adult lifespan inferred from mutant phenotype (Burnett et al., 2011) histone deacetylation inferred from direct assay (Borra et al., 2002) regulation of apoptotic process inferred from mutant phenotype (Griswold et al., 2008) regulation of histone acetylation inferred from mutant phenotype (Tulin et al., 2006) regulation of transcription, DNA-dependent inferred from direct assay (Cho et al., 2005) response to nutrient inferred from mutant phenotype (Kapahi, 2003)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Newman et al., 2002) nucleoplasm inferred from direct assay (Tulin et al., 2006) nucleus inferred from direct assay (Newman et al., 2002, Cho et al., 2005)
Terms Based on Predictions or Assertions ( 2 terms )
Molecular Function
CV term Evidence References
NAD+ binding inferred from electronic annotation with InterPro:IPR003000 (FlyBase Curators et al., 2004-) NAD-dependent histone deacetylase activity inferred from sequence or structural similarity with Saccharomyces HST1 (FlyBase, 1992-)
Biological Process ( 0 terms)
Cellular Component ( 0 terms)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Sir2 allele Gene References Sir22A-7-11 Pc (Furuyama et al., 2004) Pcl (Furuyama et al., 2004) w (Furuyama et al., 2004) Sir217 Pc (Furuyama et al., 2004) Pcl (Furuyama et al., 2004) Rpd3 (Rogina and Helfand, 2004) w (Furuyama et al., 2004) Sir205327a E(z) (Furuyama et al., 2004) h (Rosenberg and Parkhurst, 2002) RpII140 (Rosenberg and Parkhurst, 2002) Sir2EP2300 E(z) (Furuyama et al., 2004) Rpd3 (Rogina and Helfand, 2004) Sir2ex10 RpII140 (Rosenberg and Parkhurst, 2002) Sxl (Astrom et al., 2003) unspecified UbxPRE (Furuyama et al., 2004)
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 60746 DroID - A comprehensive database of gene and protein interactions. • FBgn0024291 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34708
Orthologs
OrthoDB Orthologs (51) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG68SHCH Dipteran inclusive ortholog search No orthologs identified Insect inclusive ortholog search EOG6SF81W Arthropod inclusive ortholog search EOG6PNVXT Metazoa inclusive ortholog search EOG6ND05J
Orthologs in Drosophila Species (EOG68SHCH)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Sir2     Drosophila simulans   Dsim\GD23867 Y   Drosophila sechellia   Dsec\GM10169 Y   Drosophila erecta   Dere\GG23816 Y   Drosophila yakuba   Dyak\GE18618 Y   Drosophila ananassae   Dana\GF14221 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18743 Y   Drosophila persimilis   Dper\GL24742 Y   Drosophila willistoni   Dwil\GK15314 Y   Drosophila virilis   Dvir\GJ16866 Y   Drosophila mojavensis   Dmoj\GI18096 Y   Drosophila grimshawi   Dgri\GH11392 Y
Orthologs in non-Drosophila Dipterans (None identified)
No non-Drosophilid orthologies identified
Orthologs in non-Dipteran Insects (EOG6SF81W)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Sir2   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010060   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH15906   Atta cephalotes Leafcutter ant  Acep\ACEP10949   Camponotus floridanus Florida carpenter ant  Cflo\CFLO14756   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL22386   Linepithema humile Argentine ant  Lhum\LH23569   Pogonomyrmex barbatus Red harvester ant  Pbar\PB24034   Solenopsis invicta Red fire ant  Sinv\SINV14855   Acyrthosiphon pisum Pea aphid  Apim\ACYPI008131   Pediculus humanus Human body louse  Phum\PHUM569840   Tribolium castaneum Red flour beetle  Tcas\TC002584
Orthologs in non-Insect Arthropods (EOG6PNVXT)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Arhgef3   Ixodes scapularis Deer tick  Isca\ISCW006267
Orthologs in non-Arthropod Metazoa (EOG6ND05J)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\sir-2.1 Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_016720gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018723gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_018795gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_005378gn Y Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_028113gn Y Danio rerio Zebrafish  Drer\sirt1 Y Danio rerio Zebrafish  Drer\si:dkey-103i16.6 Y Danio rerio Zebrafish  Drer\sirt3 Y Danio rerio Zebrafish  Drer\sirt2 Y Xenopus tropicalis Western clawed frog  Xtro\sirt3.2 Y Xenopus tropicalis Western clawed frog  Xtro\sirt2 Y Xenopus tropicalis Western clawed frog  Xtro\sirt1 Y Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000032567 Y Gallus gallus Domestic chicken  Ggal\SIRT3 Y Gallus gallus Domestic chicken  Ggal\SIRT1 Y Mus musculus House mouse  Mmus\Sirt2 Y Mus musculus House mouse  Mmus\Sirt1 Y Mus musculus House mouse  Mmus\Sirt3 Y Rattus norvegicus Norway rat  Rnor\Sirt3 Y Rattus norvegicus Norway rat  Rnor\Sirt2 Y Rattus norvegicus Norway rat  Rnor\ENSRNOG00000000380 Y Homo sapiens Human  Hsap\SIRT2 Y Homo sapiens Human  Hsap\SIRT3 Y Homo sapiens Human  Hsap\SIRT1 Y
Human Orthologs (3)
Gene OMIM HGNC Hsap\SIRT2     Hsap\SIRT3     Hsap\SIRT1
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\GD23867 Drosophila sechellia Dsec\GM10169 Drosophila erecta Dere\GG23816 Drosophila yakuba Dyak\GE18618 Drosophila ananassae Dana\GF14221 Drosophila pseudoobscura pseudoobscura Dpse\GA18743 Drosophila persimilis Dper\GL24742 Drosophila willistoni Dwil\GK15314 Drosophila virilis Dvir\GJ16866 Drosophila mojavensis Dmoj\GI18096 Drosophila grimshawi Dgri\GH11392
Stocks & Reagents
Stocks Listed in FlyBase ( 18 )
Bloomington	32657 Sir25.26 cn1 8838 w1118; Sir22A-7-11 24857 w1118; Sir217/SM6a 31636 y1 v1; P{TRiP.JF01423}attP2 32568 Sir24.5 cn1/SM6b, P{eve-lacZ8.0}SB1 12966 y1 w67c23; P{SUPor-P}Sir2KG00871 32481 y1 sc* v1; P{TRiP.HMS00484}attP2
Kyoto	104239 w*; P{GawB}Sir2NP2557 / CyO
VDRC	v105502 P{KK109370}VIE-260B v108241 P{KK107325}VIE-260B v23199 w1118 P{GD11580}v23199 v23201 w1118; P{GD11580}v23201
	More stocks available...
Genomic Clones ( 2 )
	BACR27F17 BACR32O12 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 166 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH25712 LD07439
Other clones	FI18008 FI19438 RE01731
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT22511 AT22531 AT31680 LD02640 LD13904 LD29911 LD32757 LD38188 RE15183 RE27621 RE31227 RE51905 RE69994 RE73041 RH05083 RH05658 RH06148 RH06189 RH06896 RH07235 RH07689 RH07793 RH08103 RH08503 RH20066 RH20490 RH21547 RH26109 RH37893 RH58682 SD08938 SD17285 SD28146
Other clones	4810 39202 44098 51365 52707 55121 65206 77897 83041 105635 118064 119252 160825 161503 190437 191184 191822 bs08d07 CK01.6C.E10 CK01.43D.A8 CK01.67A.F3 CK01.72A.F5 CK01.80A.C8 CK01.81C.D10 CK01.102D.D7 CK01.168C.H11 Dmel_dig_016518_0595_2667 Dmel_neb_007057_0591_3111 Dmel_neb_012044_0830_1945 EC33782 EC253044 EK009838 EK015806 EK037036 EK052722 EK054658 EK057123 EK064147 EK068508 EK086416 EK103430 EK123727 EK152254 EK158718 EK159421 EK162034 EK165856 EK168634 EK172519 EK182830 EK191649 EK210509 EK230852 EK239019 EK258509 EK264211 EK292848 GM10085 LD13376 RP003021904 RP003053921 RP003054604 RP003065921 RP003067493 RP003068773 RP003076536 RP003086867 RP003090347 RP003094997 RP003106476 RP003120732 RP003140281 RP003146382 RP003146399 RP003156010 RP003163050 RP003175451 RP003176071 RP003192787 RP003202134 RP003206690 RP003207907 RP003212291 RP003231754 RP003241176 RP003246352 RP003257159 RP003270674 RP003277181 RP003284935 RP003303236 RP003304197 RP003306979 RP003312955 RP003321359 RP003323232 RP003328341 RP003328746 RP003333806 RP003337494 RP003346897 RP003353353 RP003436452 RP003449738 RP003471735 RP003479901 RP003481288 RP003493676 RP003522776 RP003531818 RP003542550 RP003544769 RP003546858 RP003548720 RP003562104 RP003567077 RP003626833 RP003631002 RP003638105 RP003654205 RP003655868 RP003657715 RP003663628 RP003664420 RP003675723 RP003675934 RP003677613 SD08926
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024291 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34708
Antibody Information
	polyclonal (Bauer et al., 2009)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Sir2 CG5216 (Guan, 1999.11)
Source for database merge of	Source for merge of: Sir2 BEST:LD38188 (Barlow et al., 2001) Source for merge of: Sir2 l(2)05327 (Fernandez-Funez et al., 2000)
Additional comments
Other Comments
	DNA-protein interactions: genome-wide binding profile assayed for Sir2 protein in Kc167 cells; see Chromatin_types_NKI collection report. Individual protein-binding experiments listed under "Samples" at GEO_GSE22069 (http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE22069). (Filion et al., 2010) Sir2 plays a role in silencing mediated by the Polycomb group proteins. (Furuyama et al., 2004) Homozygosity for a Sir2 mutation has no apparent deleterious effect on viability, developmental rate or sex ratio. (Astrom et al., 2003) Histone deacetylation by Sir2 generates a transcriptionally repressed nucleoprotein complex. (Parsons et al., 2003) Sir2 is not essential for viability. (Newman et al., 2002) Sir2 is similar to S.cerevisiae SIR2 in the core domain but also has a unique 40kD C-terminal extension. Sir2 transcript is expressed maternally and throughout embryogenesis, especially within the nervous system. (Newman et al., 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_058003 RefSeq (Proteins) • NP_477351 DDBJ / EMBL / GenBank • AA264032 AAC79684 AAF53248 AAO47879 AC010214 AF068758 AI405626 AI519056 AQ073472 AW941639 AZ300944 BT004901 Entrez Gene - A searchable database of RefSeq genes. • 34708 UniProtKB/Swiss-Prot • Q9VK34 UniProtKB/TrEMBL • Q86LE9
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Sirtuin family (IPR003000) Sirtuin family, catalytic core small domain (IPR026591) Sirtuin family, catalytic core domain (IPR026590) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD07439
Linkouts
	BioGRID - A database of protein and genetic interactions • 60746 DroID - A comprehensive database of gene and protein interactions. • FBgn0024291 DRSC - Results from RNAi screens. • FBgn0024291 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024291 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG5216-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024291 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 34708 Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution • /polycomb/sir2-1.htm InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 34708 modMine - Data generated by the modENCODE project. • FBgn0024291
Synonyms & Secondary IDs ( 14 )
Reported As
Symbol Synonym	BEST:LD38188   CG5216 (Monnier et al., 2002, Sun et al., 2010, Keleman et al., 2009.8.5, Kusama et al., 2006, Ni et al., 2008, Ni et al., 2010.12.1) D.mel1 (Frye, 2000) dmSRT406 (Pandey et al., 2002) dSir2 (Orian, 2006, Partridge, 2005, Kenyon, 2005, Anonymous, 2004, Guarente and Picard, 2005, Rogina and Helfand, 2004, Shulman and Feany, 2003, Parsons et al., 2003, Zoghbi and Botas, 2002, Newman et al., 2002, Rosenberg and Parkhurst, 2002, Borra et al., 2002, Phippen et al., 2001, Fernandez-Funez et al., 2000, Metzger et al., 2007, Abed et al., 2011, Burnett et al., 2011, Longo and Kennedy, 2006, Parashar and Rogina, 2009, Bauer et al., 2009, Zhao et al., 2009, Kusama et al., 2006, Ghosh and Feany, 2004, Kanao et al., 2010) DSir2 (Miotto et al., 2006) dsir2 (Segal et al., 2004, Astrom et al., 2003, Smolik, 2009) dSIR2 (Rosenberg and Parkhurst, 2002, Barlow et al., 2001, Newman et al., 1999, Newman et al., 1998, Taylor-Harding et al., 2004) l(2)05326   SIR2 (Chopra and Mishra, 2005, Furuyama et al., 2001, Schwartz and Pirrotta, 2007, Tie et al., 2009, van Steensel et al., 2010, Filion et al., 2010) Sir2 (Skorupa et al., 2007, Bauer et al., 2007, Cho et al., 2005, Zaratiegui, 2007, Hochwagen, 2006, Guarente, 2006, Buszczak et al., 2007, Gan et al., 2010, Sun et al., 2010, Wood et al., 2004, Longo and Kennedy, 2006, Gambini et al., 2011, Antosh et al., 2011, Das et al., 2009, Avery et al., 2009, Shevelyov et al., 2009, Kusama et al., 2006, Balan et al., 2008, Branco et al., 2008, Pallos et al., 2008, Bass et al., 2007, de Wit et al., 2008, Morozova et al., 2006, Lorbeck et al., 2010, Kong et al., 2010, Reis et al., 2010, Pushpavalli et al., 2012, Japanese National Institute of Genetics, 2012.5.21) sir2 (Wei and Rong, 2007, Griswold et al., 2008, Rong, 2008, Smolik, 2009, Pek et al., 2012) Sir2L (Tulin and Spradling, 2004)
Name Synonym	Silent information regulator 2 (Lorbeck et al., 2010) Sir2
Secondary FlyBase IDs
	FBgn0046250
References ( 126 )
Generate a list of	All references relating to this gene ( 126 )
List References by type	Research paper  ( 65 ) Supplementary material  ( 5 ) Review  ( 15 ) Personal communication to FlyBase  ( 13 ) Abstract  ( 19 ) FlyBase analysis  ( 3 ) Stock list  ( 1 ) Curated genome annotation  ( 2 ) DNA/RNA sequence record  ( 1 ) Website  ( 1 ) Letter  ( 1 )
Recent research papers ( 10 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Koh et al., 2012, J. Biol. Chem. 287(16): 12750--12758 Silent Information Regulator 2 (Sir2) and Forkhead Box O (FOXO) Complement Mitochondrial Dysfunction and Dopaminergic Neuron Loss in Drosophila PTEN-induced Kinase 1 (PINK1) Null Mutant. [FBrf0218041] Pek et al., 2012, Development 139(24): 4505--4513 Polo-mediated phosphorylation of Maelstrom regulates oocyte determination during oogenesis in Drosophila. [FBrf0220058] Pushpavalli et al., 2012, Bioorg. Med. Chem. Lett. 22(20): 6418--6424 Imidazo-benzothiazoles a potent microRNA modulator involved in cell proliferation. [FBrf0219513] Abed et al., 2011, EMBO J. 30(7): 1289--1301 Degringolade, a SUMO-targeted ubiquitin ligase, inhibits Hairy/Groucho-mediated repression. [FBrf0213366] Antosh et al., 2011, Cell Cycle 10(6): 904--911 Comparative transcriptional pathway bioinformatic analysis of dietary restriction, Sir2, p53 and resveratrol life span extension in Drosophila. [FBrf0213419] Burnett et al., 2011, Nature 477(7365): 482--485 Absence of effects of Sir2 overexpression on lifespan in C. elegans and Drosophila. [FBrf0215823] Gambini et al., 2011, Arch. Biochem. Biophys. 512(1): 24--29 Free [NADH]/[NAD(+)] regulates sirtuin expression. [FBrf0214039] Kirilly et al., 2011, Neuron 72(1): 86--100 Intrinsic epigenetic factors cooperate with the steroid hormone ecdysone to govern dendrite pruning in Drosophila. [FBrf0216319] Mulligan et al., 2011, Mol. Cell 42(5): 689--699 A SIRT1-LSD1 Corepressor Complex Regulates Notch Target Gene Expression and Development. [FBrf0213865] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...