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Gene Dmel\Nach

General Information
Symbol	Dmel\Nach	Species	D. melanogaster
Name	Nach	Annotation symbol	CG8178
Feature type	protein_coding_gene	FlyBase ID	FBgn0024319
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	ppk4
Genomic Location
Chromosome (arm)	2R	Recombination map
Cytogenetic map	53C14-53C14	Sequence location	2R:12,540,710..12,542,551 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents modENCODE GBrowse	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Nach is referred to in FlyBase by the symbol Dmel\Nach (CG8178, FBgn0024319). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: sodium channel activity. There is experimental evidence that it is involved in the biological process: liquid clearance, open tracheal system. 5 alleles are reported. The phenotype of these alleles is annotated with: adult tracheal system. It has 3 annotated transcripts and 3 annotated polypeptides. Protein features are: Na+ channel, amiloride-sensitive; Na+ channel, amiloride-sensitive, conserved site. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed in adult female stages. Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. Comments on Affy2 ProbeSet: ProbeSet 1623711_at completely aligns to an exonic region common to each of the 3 FlyBase-annotated transcript isoforms of Nach. Gene sequence location is 2R:12540710..12542551.
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2011_10
FB2012_01	Sequence features BKN28198 HFA07478
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 53C14-53C14   Limits computationally determined from genome sequence between P{PZ}Sema-2a03021&P{PZ}Cdk405428 and P{EP}BEST:SD02913EP2148&P{lacW}Dekk09907
Experimentally Determined Cytological Location
Cytogenetic map Notes References 53C11-53C13     (Welsh, 2003.1.15) 53D1-53D3     (Da Lage, 1997.9.3)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Nach for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Nach-RA FBtr0087116  NM_137301   1551   516 Nach-RB FBtr0089587  NM_206137   1608   535 Nach-RC FBtr0089588  NM_206136   1662   553
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Nach-PA   FBpp0086262   60.0   516   8.02     NP_611145   AAF57970 Nach-PB   FBpp0088960   62.3   535   8.11     NP_995859   AAS64839 Nach-PC   FBpp0088959   64.4   553   8.25     NP_995858   AAS64840
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Na+ channel, amiloride-sensitive (IPR001873) Na+ channel, amiloride-sensitive, conserved site (IPR020903)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE013599 AAF57970   AE013599 AAS64839   AE013599 AAS64840   AF022713 AAD09149   AY226538 AAO47364
UniProtKB/Swiss-Prot	O61365
UniProtKB/TrEMBL
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 15 -- 17   trachea   (Liu et al., 2003) embryonic/larval dorsal trunk   (Liu et al., 2003) embryonic stage 16 -- 17   embryonic/larval transverse connective   (Liu et al., 2003) embryonic stage 17   secondary trachea   (Liu et al., 2003) tracheal tip cell   (Liu et al., 2003)
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization
CV Term
Notes
High-Throughput Expression Data
or
See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes. modENCODE Temporal Expression Data for FBgn0024319    Styles Linear Logarithmic Heatmap    Scales max expr for FBgn0024319 Very low expression bin max Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very low expression to a trough of no expression detected. Peak expression observed in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No expression (0 - 0)   Extremely low expression (1 - 10)   Very low expression (11 - 100)   Low expression (101 - 400)   Moderate expression (401 - 1400)   Moderately high expression (1401 - 4000)   High expression (4001 - 10000)   Very high expression (10001 - 100000)   Extremely high expression (100001 - 2000000) Linear, scaled to maximum FBgn0024319 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low  Linear, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  log, scaled to maximum FBgn0024319 expression level Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low  log, scaled to Very low expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    0 embryo 02-04hr    0 embryo 04-06hr    0 embryo 06-08hr    0 embryo 08-10hr    0 embryo 10-12hr    0 embryo 12-14hr    0 embryo 14-16hr    1 embryo 16-18hr    5 embryo 18-20hr    4 embryo 20-22hr    0 embryo 22-24hr    5 larva L1    2 larva L2    2 larva L3 12hr old    2 larva L3 puffstage 1-2    0 larva L3 puffstage 3-6    0 larva L3 puffstage 7-9    2 white prepupae new    3 white prepupae 12hr    1 white prepupae 24hr    1 pupae 2d postWPP    2 pupae 3d postWPP    3 pupae 4d postWPP    4 adult male 01day    4 adult male 05day    1 adult male 30day    3 adult female 01day    3 adult female 05day    25 adult female 30day    24 Expression Level Scale  None   Extremely low   Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     FlyAtlas Anatomical Expression Data for FBgn0024319    Styles Linear Logarithmic Heatmap Back-to-back    Scales max expr for FBgn0024319 Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: No expression detected in any larval or adult organs/tissues. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (1000 - 25000) Linear, scaled to maximum FBgn0024319 expression level Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to maximum FBgn0024319 expression level Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    2.125 Larval Midgut    3.4 Larval Hindgut    5.1 Larval Malpighian Tubules    8.3 Larval Fat Body    9.6 Larval Salivary Gland    1.8 Larval Trachea    1 Larval Carcass    1.075 Adult Head    4.4 Adult Eye    3.025 Adult Brain    0.8 Adult Thoracic-Abdominal Ganglion    2.9 Adult Crop    1.1 Adult Midgut    5 Adult Hindgut    4.5 Adult Malpighian Tubules    6.1 Adult Fat Body    6.9 Adult Salivary Gland    5.3 Adult Heart    0.625 Adult VirginFemale Spermatheca    2.8 Adult InseminatedFemale Spermatheca    1.1 Adult Ovary    4.8 Adult Testis    1.6 Adult Male Accessory Gland    7.7 Adult Carcass    1.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    4.4   NA  Eye    3.025   NA  Brain    0.8   2.125  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    2.9   NA  Crop    1.1   3.4  Midgut    5   5.1  Hindgut    4.5   8.3  Malpighian Tubules    6.1   9.6  Fat Body    6.9   1.8  Salivary Gland    5.3   NA  Heart    0.625   1  Trachea    NA   NA  VirginFemale Spermatheca    2.8   NA  InseminatedFemale Spermatheca    1.1   NA  Ovary    4.8   NA  Testis    1.6   NA  Male Accessory Gland    7.7   1.075  Carcass    1.6 modENCODE Temporal Expression Data (Graveley et al., 2011) FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
Expression Clusters
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024319 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8178-RA
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable, with Scer\GAL4pnr-MD237 NachGD2039 Phenotype manifest in Allele adult tracheal system NachdsRNA.cLa
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Nach Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Nach Class Mutagen Stocks Known lesion NachGD2039 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes NachJF02566 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes NachKK108198 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes NachDN.Scer\UAS in vitro construct - regulatory fusion 0 Yes NachdsRNA.cLa in vitro construct - RNAi 0 Yes
Aneuploid Aberrations
Disrupted in	Df(2R)Exel6064 (Ryder, 2004.4.26) Df(2R)Exel7144 (Ryder, 2004.4.26)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD2039} NA P{KK108198} NA P{TRiP.JF02566} NA P{UAS-Nach.DN} NA characterization construct P{Nach-GAL4.L} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 5 unique terms )
Terms Based on Experimental Evidence ( 1 term )
Molecular Function ( 0 terms)
Biological Process
CV term Evidence References
liquid clearance, open tracheal system inferred from mutant phenotype (assigned by UniProtKB) (Liu et al., 2003)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 4 terms )
Molecular Function
CV term Evidence References
sodium channel activity inferred from electronic annotation with InterPro:IPR001873, InterPro:IPR020903 (FlyBase Curators et al., 2004-) non-traceable author statement (Da Lage, 1997.9.3, Welsh, 2003.1.15) non-traceable author statement (assigned by UniProtKB) (Liu et al., 2003)
Biological Process
CV term Evidence References
sodium ion transport inferred from electronic annotation with InterPro:IPR001873, InterPro:IPR020903 (FlyBase Curators et al., 2004-)
Cellular Component
CV term Evidence References
integral to membrane non-traceable author statement (assigned by UniProtKB) (Liu et al., 2003) membrane inferred from electronic annotation with InterPro:IPR001873, InterPro:IPR020903 (FlyBase Curators et al., 2004-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
Protein-protein
Interacting group Assay References
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Nach allele Gene References
External Data
Linkouts	DroID - A comprehensive database of gene and protein interactions. • FBgn0024319
Orthologs
Genome-wide drosophilid orthologs	Dana\Nach Dere\GG22253 Dgri\GH22707 Dmoj\GI18494 Dper\GL11823 Dpse\GA20871 Dsec\GM20042 Dsim\GD25525 Dvir\Nach Dwil\GK10729 Dyak\GE14047
Curated drosophilid orthologs	Dana\Nach (Da Lage, 1997.9.10) Dpse\GA20871   Dvir\Nach
Linkouts	InParanoid A subset of ortholog calls from InParanoid. • XP_001121909.1 Apis mellifera (honey bee) ENSBTAP00000013412 Bos taurus (bovine) CBP27913 Caenorhabditis briggsae (nematode) CE26381 Caenorhabditis elegans (worm) RP43435 Caenorhabditis remanei (nematode) ENSGALP00000022994 Gallus gallus (chicken) ENSP00000300061 Homo sapiens (human) ENSMMUP00000007522 Macaca mulatta (rhesus monkey) ENSMUSP00000000221 Mus musculus (house mouse) XP_001603759.1 Nasonia vitripennis (wasp) ENSPPYP00000008126 Pongo pygmaeus (orangutan) ENSPTRP00000013457 Pan troglodytes (chimpanzee) ENSRNOP00000024057 Rattus norvegicus (norway rat) ENSXETP00000027163 Xenopus tropicalis (frog) OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0231675 FBgn0212742 FBgn0042730 FBgn0196812 FBgn0174926 JGI_V11_313302 FBgn0080861 FBgn0149431 FBgn0141233 FBgn0130165 FBgn0114426 FBgn0024473 BGIBMGA013697
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	27262 y1 v1; P{TRiP.JF02566}attP2
VDRC	v106647 P{KK108198}VIE-260B v45920 w1118; P{GD2039}v45920 v45921 w1118; P{GD2039}v45921
Genomic Clones ( 1 )
	BACR36P23 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 0 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024319 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36864
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of
Source for database merge of
Additional comments
Other Comments
	Nach may have a role in liquid clearance from the tracheal system. (Liu et al., 2003)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_137301 NM_206136 NM_206137 RefSeq (Proteins) • NP_611145 NP_995858 NP_995859 DDBJ / EMBL / GenBank • AAD09149 AAF57970 AAO47364 AAS64839 AAS64840 AF022713 AY226538 Entrez Gene - A searchable database of RefSeq genes. • 36864 UniProtKB/Swiss-Prot • O61365
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Na+ channel, amiloride-sensitive (IPR001873) Na+ channel, amiloride-sensitive, conserved site (IPR020903)
Linkouts
	DroID - A comprehensive database of gene and protein interactions. • FBgn0024319 DRSC - Results from RNAi screens. • FBgn0024319 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024319 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG8178-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024319 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 36864 InParanoid A subset of ortholog calls from InParanoid. • XP_001121909.1 Apis mellifera (honey bee) ENSBTAP00000013412 Bos taurus (bovine) CBP27913 Caenorhabditis briggsae (nematode) CE26381 Caenorhabditis elegans (worm) RP43435 Caenorhabditis remanei (nematode) ENSGALP00000022994 Gallus gallus (chicken) ENSP00000300061 Homo sapiens (human) ENSMMUP00000007522 Macaca mulatta (rhesus monkey) ENSMUSP00000000221 Mus musculus (house mouse) XP_001603759.1 Nasonia vitripennis (wasp) ENSPPYP00000008126 Pongo pygmaeus (orangutan) ENSPTRP00000013457 Pan troglodytes (chimpanzee) ENSRNOP00000024057 Rattus norvegicus (norway rat) ENSXETP00000027163 Xenopus tropicalis (frog) modMine - Data generated by the modENCODE project. • FBgn0024319 OrthoDB (Arthropod subset) The hierarchical catalog of eukaryotic orthologs. • FBgn0231675 FBgn0212742 FBgn0042730 FBgn0196812 FBgn0174926 JGI_V11_313302 FBgn0080861 FBgn0149431 FBgn0141233 FBgn0130165 FBgn0114426 FBgn0024473 BGIBMGA013697
Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	CG8178 (Keleman et al., 2009.8.5) Nach (Perkins et al., 2009.8.10, Di Stefano et al., 2007) ppk4 (Liu et al., 2003) PPK4 (Liu et al., 2003, Welsh, 2003.1.15)
Name Synonym	Nach   pickpocket 4 (Welsh, 2003.1.15)
Secondary FlyBase IDs
References ( 19 )
Research paper	Di Stefano et al., 2007, Curr. Biol. 17(9): 808--812 Mutation of Drosophila Lsd1 disrupts H3-K4 methylation, resulting in tissue-specific defects during development. [FBrf0202084] Liu et al., 2003, Neuron 39(1): 133--146 Contribution of Drosophila DEG/ENaC genes to salt taste. [FBrf0160745] Liu et al., 2003, Proc. Natl. Acad. Sci. USA 100(4): 2128--2133 Drosophila DEG/ENaC pickpocket genes are expressed in the tracheal system, where they may be involved in liquid clearance. [FBrf0159284] Giannakou and Dow, 2001, J. exp. Biol. 204(21): 3703--3716 Characterization of the Drosophila melanogaster alkali-metal/proton exchanger (NHE) gene family. [FBrf0141626] Loren et al., 2001, Genes to Cells 6(6): 531--544 Identification and characterization of DAlk: a novel Drosophila melanogaster RTK which drives ERK activation in vivo. [FBrf0137243] Da Lage et al., 1998, Proc. Natl. Acad. Sci. USA 95(12): 6848--6853 Amyrel, a paralogous gene of the amylase gene family in Drosophila melanogaster and the Sophophora subgenus. [FBrf0102910]
Supplementary material	Mummery-Widmer et al., 2009, Nature 458(7241): Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Personal communication to FlyBase	Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510] Perkins et al., 2009.8.10, Update to TRiP stock collection. Update to TRiP stock collection. [FBrf0208863] Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Ryder, 2004.4.26, Exelixis CG deletion data. Exelixis CG deletion data. [FBrf0184335]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
DNA/RNA sequence record	Welsh, 2003.1.15, GenBank/EMBL/DDBJ: AY226538 Drosophila melanogaster pickpocket 4 long form (PPK4) mRNA, complete cds. [FBrf0158510] Da Lage, 1997.9.3, GenBank/EMBL/DDBJ: AF022713 Drosophila melanogaster putative inorganic phosphate cotransporter (Picot) gene, partial cds; putative sodium channel (Nach) and putative amylase-related protein (Amyrel) genes, complete cds; and putative serine-enriched protein (gprs) gene, partial cds. [FBrf0114358] Da Lage, 1997.9.10, GenBank/EMBL/DDBJ: AF024691 Drosophila ananassae putative inorganic phosphate cotransporter (picot), putative sodium channel (NaCh), and putative AMYREL protein (Amy c6) genes, complete cds. [FBrf0114357]
Curated genome annotation	Butler, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126656]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]