FB2020_01, released Feb 12, 2020

Gene: Dmel\Npc1a

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General Information
Symbol
Dmel\Npc1a
Species
D. melanogaster
Name
Niemann-Pick type C-1a
Annotation Symbol
CG5722
Feature Type
protein_coding_gene
FlyBase ID
FBgn0024320
Gene Model Status
Current
Stock Availability
19 publicly available
Gene Snapshot
Niemann-Pick type C-1a (Npc1a) encodes a cholesterol trafficking protein that contributes to sterol and ecdysone metabolism. It is involved in sperm individualization. [Date last reviewed: 2019-09-26]
Other Summaries
Also Known As

NPC1, Niemann-Pick Type C-1, dnpc1a

Key Links
Genomic Location
Cytogenetic map
31B1-31B1
Sequence location
2L:10,213,812..10,221,216 [-]
Recombination map

2-40

RefSeq locus
NT_033779 REGION:10213812..10221216
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
GO Summary Ribbons
Protein Family (UniProt)
-
Protein Signatures (InterPro)
Molecular Function (GO)
[Detailed GO annotations]
Experimental Evidence
-
Predictions / Assertions
lipid transporter activity
Summaries
Summary (Interactive Fly)

a transmembrane protein related to the Hedgehog receptor Patched - mutants have a shortage of sterol, and, as a consequence, inadequate ecdysone synthesis - required in the maintenance of neuronal function and viability - loss of NPC1a in neurons mimics the human Niemann-Pick disease type C neurodegenerative condition

Gene Model and Products
Number of Transcripts
7
Number of Unique Polypeptides
2

Please see the GBrowse view of Dmel\Npc1a or the JBrowse view of Dmel\Npc1a for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Comments on Gene Model

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
nuclear_gene
protein_coding_gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Npc1a-RA
FBtr0079957
NM_135513
4223
1287
Npc1a-RB
FBtr0303905
NM_001201840
4119
1287
Npc1a-RC
FBtr0303906
NM_001201841
4081
1287
Npc1a-RD
FBtr0303907
NM_001201842
4127
1287
Npc1a-RE
FBtr0343608
NM_001298866
4211
1283
Npc1a-RF
FBtr0343609
NM_001298867
4032
1283
Npc1a-RG
FBtr0343610
NM_001298868
5025
1283
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
RefSeq ID
GenBank
Npc1a-PA
FBpp0079547
143.3
1287
5.27
NP_609357
AAF52874
Npc1a-PB
FBpp0292908
143.3
1287
5.27
NP_001188769
ADV37019
Npc1a-PC
FBpp0292909
143.3
1287
5.27
NP_001188770
ADV37020
Npc1a-PD
FBpp0292910
143.3
1287
5.27
NP_001188771
ADV37021
Npc1a-PE
FBpp0310203
142.9
1283
5.27
NP_001285795
AHN54309
Npc1a-PF
FBpp0310204
142.9
1283
5.27
NP_001285796
AHN54310
Npc1a-PG
FBpp0310205
142.9
1283
5.27
NP_001285797
AHN54311
Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1287 aa isoforms: Npc1a-PA, Npc1a-PB, Npc1a-PC, Npc1a-PD
1283 aa isoforms: Npc1a-PE, Npc1a-PF, Npc1a-PG
Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Npc1a using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Gene Ontology (7 terms)
Molecular Function (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
lipid transporter activity
inferred from electronic annotation with InterPro:IPR004765
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Biological Process (5 terms)
Terms Based on Experimental Evidence (5 terms)
CV Term
Evidence
References
ecdysteroid biosynthetic process
inferred from mutant phenotype
(Huang et al., 2007)
molting cycle, chitin-based cuticle
inferred from mutant phenotype
(Huang et al., 2005)
regulation of cholesterol transport
inferred from direct assay
(Phillips et al., 2008)
sperm individualization
inferred from mutant phenotype
(Wang et al., 2011)
sterol metabolic process
inferred from mutant phenotype
(Huang et al., 2005)
Terms Based on Predictions or Assertions (0 terms)
Cellular Component (1 term)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (1 term)
CV Term
Evidence
References
integral component of membrane
inferred from electronic annotation with InterPro:IPR000731, InterPro:IPR004765
(assigned by InterPro )
(FlyBase Curators et al., 2004-)
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
in situ
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 1 -- 17
organism | ubiquitous

Comment: transcript is uniformly distributed

(Fluegel et al., 2006)
embryonic stage 13
ring gland
(Xiang et al., 2010)
northern blot
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage -- adult stage
organism
(Fluegel et al., 2006)
Additional Descriptive Data

Npc1a transcript is uniformly distributed during embryonic stages, and expressed at all stages of development.

(Fluegel et al., 2006)
Marker for
 
Subcellular Localization
CV Term
Polypeptide Expression
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
Reporter: P{Npc1a-GAL4.2.3}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
ring gland
(Xiang et al., 2010)
Reporter: P{Npc1a-GAL4.RSE}
Stage
Tissue/Position (including subcellular localization)
Reference
third instar larval stage
ring gland
(Xiang et al., 2010)
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\Npc1a in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
FLIGHT - Cell culture data for RNAi and other high-throughput technologies
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyBase Wiki
Image Based Resources
Alleles, Insertions, and Transgenic Constructs
Classical and Insertion Alleles ( 19 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 12 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Npc1a
Transgenic constructs containing regulatory region of Npc1a
Deletions and Duplications ( 3 )
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
lethal (with Df(2L)BSC143)
lethal (with Df(2L)J2)
lethal | first instar larval stage | recessive
lethal | pupal stage | recessive
lethal | recessive | second instar larval stage
lethal | recessive | third instar larval stage
lethal - all die before end of first instar larval stage
lethal - all die before end of first instar larval stage (with Npc1a1)
lethal - all die before end of first instar larval stage (with Npc1a2)
lethal - all die before end of first instar larval stage | recessive
lethal - all die before end of larval stage | recessive
viable, with Scer\GAL4- :
viable, with Dcr-2UAS.cDa, Scer\GAL4elav.PLu
viable, with Scer\GAL4Mef2.PR
viable, with Scer\GAL4pnr-MD237
viable, with Scer\GAL4tin.CΔ4
Sterility
Allele
male sterile, with Npc1aUAS.EYFP, Scer\GAL42-286
male sterile, with Npc1aunspecified, Scer\GAL42-286
Other Phenotypes
Allele
aging defective
decreased fecundity | male, with Scer\GAL42-286
developmental rate defective, with Scer\GAL4P0206
developmental rate defective, with Scer\GAL4phm.PO
eclosion defective
large body | pupal stage, with Scer\GAL4P0206
locomotor behavior defective | adult stage
neuroanatomy defective | adult stage
neuroanatomy defective | adult stage | progressive
neuroanatomy defective | adult stage | progressive, with Scer\GAL4elav-C155
neurophysiology defective | adult stage
some die during first instar larval stage
some die during first instar larval stage (with Npc1a1)
some die during first instar larval stage (with Npc1a2)
some die during first instar larval stage | recessive
some die during third instar larval stage | recessive
wild-type
Phenotype manifest in
Allele
adult brain | adult stage
endosome, with Scer\GAL4phm.PO
eye | adult stage
individualization stage spermatid, with Npc1aUAS.EYFP, Scer\GAL42-286
individualization stage spermatid, with Npc1aunspecified, Scer\GAL42-286
neuropil | adult stage
prothoracic gland | third instar larval stage, with Scer\GAL4P0206
prothoracic gland | third instar larval stage, with Scer\GAL4phm.PO
retina | adult stage
rhabdomere | adult stage
seminal vesicle, with Npc1aUAS.EYFP, Scer\GAL42-286
seminal vesicle, with Npc1aunspecified, Scer\GAL42-286
sperm individualization complex, with Npc1aUAS.EYFP, Scer\GAL42-286
sperm individualization complex, with Npc1aunspecified, Scer\GAL42-286
Orthologs
Downloads
Download All DIOPT Orthologs
Download All OrthoDB Orthologs
Human Orthologs (via DIOPT v7.1)
Homo sapiens (Human) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Hsap\NPC1
13 of 15
Yes
Yes
Hsap\NPC1L1
6 of 15
No
Yes
Hsap\SCAP
1 of 15
No
No
Model Organism Orthologs (via DIOPT v7.1)
Mus musculus (laboratory mouse) (3)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
eggNOG
Hieranoid
Homologene
Inparanoid
Isobase
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
RoundUp
TreeFam
ZFIN
Alignment
Complementation?
Transgene?
Mmus\Npc1
13 of 15
Yes
Yes
Mmus\Npc1l1
6 of 15
No
Yes
Mmus\Scap
1 of 15
No
No
Rattus norvegicus (Norway rat) (3)
Rnor\Npc1
10 of 13
Yes
Yes
Rnor\Npc1l1
3 of 13
No
No
Rnor\Scap
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (3)
Xtro\npc1
6 of 12
Yes
Yes
Xtro\npc1l1
3 of 12
No
Yes
Xtro\scap
1 of 12
No
No
Danio rerio (Zebrafish) (3)
Drer\npc1
11 of 15
Yes
Yes
Drer\npc1l1
4 of 15
No
Yes
Drer\scap
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (2)
Cele\ncr-1
11 of 15
Yes
No
Cele\ncr-2
8 of 15
No
No
Arabidopsis thaliana (thale-cress) (2)
Atha\AT4G38350
6 of 9
Yes
Yes
Atha\AT1G42470
5 of 9
No
Yes
Saccharomyces cerevisiae (Brewer's yeast) (1)
Scer\NCR1
13 of 15
Yes
Yes
Schizosaccharomyces pombe (Fission yeast) (0)
No records found.
Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG091900VY )
Organism
Common Name
Gene
AAA Syntenic Ortholog
Multiple Dmel Genes in this Orthologous Group
Drosophila melanogaster
fruit fly
Dmel\Npc1a
Drosophila suzukii
Spotted wing Drosophila
Dsuz\DS10_00000447
Drosophila simulans
Dsim\GD22294
Drosophila sechellia
Dsec\GM11941
Drosophila erecta
Dere\GG23970
Drosophila yakuba
Dyak\GE26328
Drosophila ananassae
Dana\GF14130
Drosophila pseudoobscura pseudoobscura
Dpse\GA25612
Drosophila virilis
Dvir\GJ18108
Drosophila mojavensis
Dmoj\GI18274
Drosophila grimshawi
Dgri\GH13378
Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091500H5 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Musca domestica
House fly
Mdoa\17014678
Musca domestica
House fly
Mdoa\17024265
Glossina morsitans
Tsetse fly
Gmor\GMOY010270
Glossina morsitans
Tsetse fly
Gmor\GMOY002049
Lucilia cuprina
Australian sheep blowfly
Lcup\FF38_08565
Mayetiola destructor
Hessian fly
Mdes\Mdes009410
Mayetiola destructor
Hessian fly
Mdes\Mdes009014
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL003325
Aedes aegypti
Yellow fever mosquito
Aaeg\AAEL009531
Anopheles darlingi
American malaria mosquito
Adar\ADAC010311
Anopheles darlingi
American malaria mosquito
Adar\ADAC009511
Anopheles gambiae
Malaria mosquito
Agam\AGAP000579
Anopheles gambiae
Malaria mosquito
Agam\AGAP008137
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ014970
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ004884
Culex quinquefasciatus
Southern house mosquito
Cqui\CPIJ008198
Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W00ZY )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Bombyx mori
Silkmoth
Bmor\BGIBMGA009049
Danaus plexippus
Monarch butterfly
Dple\DPOGS200982-PA
Heliconius melpomene
Postman butterfly
Hmel\HMEL003602
Heliconius melpomene
Postman butterfly
Hmel\HMEL011825
Apis florea
Little honeybee
Aflo\2274542308
Apis florea
Little honeybee
Aflo\2147516659
Apis mellifera
Western honey bee
Amel\GB43203
Apis mellifera
Western honey bee
Amel\LOC408992
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP21839
Bombus impatiens
Common eastern bumble bee
Bimp\BIMP25364
Bombus terrestris
Buff-tailed bumblebee
Bter\15207339
Bombus terrestris
Buff-tailed bumblebee
Bter\15211606
Linepithema humile
Argentine ant
Lhum\LH23787
Linepithema humile
Argentine ant
Lhum\LH16950
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0000d8
Megachile rotundata
Alfalfa leafcutting bee
Mrot\MROTU:0027f5
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG012199
Nasonia vitripennis
Parasitic wasp
Nvit\Nasvi2EG013236
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_03479
Dendroctonus ponderosae
Mountain pine beetle
Dpod\YQE_12054
Tribolium castaneum
Red flour beetle
Tcas\GLEAN_05088
Tribolium castaneum
Red flour beetle
Tcas\TC033918
Pediculus humanus
Human body louse
Phum\PHUM472470
Pediculus humanus
Human body louse
Phum\PHUM580700
Rhodnius prolixus
Kissing bug
Rpro\RPRC000480
Rhodnius prolixus
Kissing bug
Rpro\RPRC000135
Cimex lectularius
Bed bug
Clec\CLEC001534
Cimex lectularius
Bed bug
Clec\CLEC011363
Acyrthosiphon pisum
Pea aphid
Apim\ACYPI009020
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_04513
Zootermopsis nevadensis
Nevada dampwood termite
Znev\Znev_01453
Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X00Y6 )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strigamia maritima
European centipede
Smar\SMAR008157
Strigamia maritima
European centipede
Smar\SMAR001453
Strigamia maritima
European centipede
Smar\SMAR003276
Ixodes scapularis
Black-legged tick
Isca\ISCW020300
Ixodes scapularis
Black-legged tick
Isca\ISCW006533
Stegodyphus mimosarum
African social velvet spider
Smim\KFM58254.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM77574.1
Stegodyphus mimosarum
African social velvet spider
Smim\KFM60760.1
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g02790
Tetranychus urticae
Two-spotted spider mite
Turt\tetur02g03010
Tetranychus urticae
Two-spotted spider mite
Turt\tetur05g01210
Tetranychus urticae
Two-spotted spider mite
Turt\tetur136g00010
Tetranychus urticae
Two-spotted spider mite
Turt\tetur53g00100
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_321920
Daphnia pulex
Water flea
Dpux\DAPPUDRAFT_328579
Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G00JD )
Organism
Common Name
Gene
Multiple Dmel Genes in this Orthologous Group
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-Npc1l1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-NiempC1
Strongylocentrotus purpuratus
Purple sea urchin
Spur\Sp-NiempC1B
Strongylocentrotus purpuratus
Purple sea urchin
Spur\SPU_009669gn
Ciona intestinalis
Vase tunicate
Cint\ENSCING00000004214
Ciona intestinalis
Vase tunicate
Cint\npc1b
Gallus gallus
Domestic chicken
Ggal\NPC1
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v7.1)
Drosophila melanogaster (Fruit fly) (4)
Gene Symbol
Score
Source
Compara
eggNOG
Homologene
Inparanoid
Isobase
OrthoDB
OrthoFinder
orthoMCL
Panther
RoundUp
Alignment
Npc1b
6 of 10
ptc
1 of 10
Ptr
1 of 10
SCAP
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 3 )
Potential Models Based on Orthology ( 1 )
Modifiers Based on Experimental Evidence ( 1 )
Allele
Disease
Interaction
References
Npc1aKK101077
model of  Niemann-Pick disease
is ameliorated by Tsc1UAS.cTa
(Danielsen et al., 2016)
is ameliorated by gigUAS.cTa
(Danielsen et al., 2016)
is ameliorated by Atg1UAS.cSa
(Danielsen et al., 2016)
is ameliorated by Atg13UAS.cCa
(Danielsen et al., 2016)
Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Homo sapiens (Human)
Gene name
Score
OMIM
OMIM Phenotype
DO term
Complementation?
Transgene?
NPC1; NPC intracellular cholesterol transporter 1
13 of 15
607623
NPC1L1; NPC1 like intracellular cholesterol transporter 1
6 of 15
608010
    Functional Complementation Data
    Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
    Interactions
    Summary of Physical Interactions
    esyN Network Diagram
    Interactions Browser
    Summary of Genetic Interactions
    esyN Network Diagram
    esyN Network Key:
    Suppression
    Enhancement
    View in Interactions Browser
    Please look at the allele data for full details of the genetic interactions
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    Npc1a
    suppressible
    Atg13|Atg1
    (Danielsen et al., 2016)
    Npc1a
    suppressible
    sit
    (Danielsen et al., 2016)
    Npc1a
    suppressible
    Tsc1|gig
    (Danielsen et al., 2016)
    Starting gene(s)
    Interaction type
    Interacting gene(s)
    Reference
    External Data
    Linkouts
    DroID - A comprehensive database of gene and protein interactions.
    InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
    Pathways
    Signaling Pathways (FlyBase)
    Metabolic Pathways
    External Data
    Linkouts
    KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
    Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
    Genomic Location and Detailed Mapping Data
    Chromosome (arm)
    2L
    Recombination map

    2-40

    Cytogenetic map
    31B1-31B1
    Sequence location
    2L:10,213,812..10,221,216 [-]
    FlyBase Computed Cytological Location
    Cytogenetic map
    Evidence for location
    31B1-31B1
    Limits computationally determined from genome sequence between P{lacW}l(2)k06324k06324 and P{lacW}me31Bk06607
    Experimentally Determined Cytological Location
    Cytogenetic map
    Notes
    References
    31B2-31B3
    (Zhang, 1999.9.16)
    31B1-31B5
    (determined by in situ hybridisation)
    (Johnson, 1998.3.13)
    Experimentally Determined Recombination Data
    Location

    2-40

    (FlyBase, 2016)

    2-34.8

    (Comeron et al., 2012)
    Left of (cM)
    Right of (cM)
    Notes
    Stocks and Reagents
    Stocks (19)
    Genomic Clones (20)
    cDNA Clones (54)
     

    Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

    cDNA clones, fully sequences
    BDGP DGC clones
    Other clones
      Drosophila Genomics Resource Center cDNA clones

      For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

      cDNA Clones, End Sequenced (ESTs)
      BDGP DGC clones
      Other clones
      RNAi and Array Information
      Linkouts
      DRSC - Results frm RNAi screens
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      Antibody Information
      Laboratory Generated Antibodies
       
      Commercially Available Antibodies
       
      Other Information
      Relationship to Other Genes
      Source for database identify of

      Source for identity of: Npc1a NPC1

      (Huang et al., 2007)
      Source for database merge of
      Additional comments
      Other Comments

      Npc1a mutants rescued to adults by restoration of ecdysone production mimic human NPC patients with progressive motor defects and reduced life spans. Npc1a mutant brains reveal age-progressive accumulation of multilamellar and multivescicular organelles, preceding the onset of neurodegeneration.

      (Phillips et al., 2008)

      The LD15757 cDNA clone contains a complete NPC1 open reading frame along with contaminating bacterial sequence.

      (Fluegel et al., 2006)

      dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R+ cell morphology.

      (Kiger et al., 2003)
      Origin and Etymology
      Discoverer
      Etymology
      Identification
      External Crossreferences and Linkouts ( 55 )
      Sequence Crossreferences
      NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
      GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
      GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
      RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
      UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information
      Other crossreferences
      Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
      Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
      Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
      Flygut - An atlas of the Drosophila adult midgut
      GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
      iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
      InterPro - A database of protein families, domains and functional sites
      KEGG Genes - Molecular building blocks of life in the genomic space.
      modMine - A data warehouse for the modENCODE project
      Linkouts
      DroID - A comprehensive database of gene and protein interactions.
      DRSC - Results frm RNAi screens
      FLIGHT - Cell culture data for RNAi and other high-throughput technologies
      FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
      FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
      FlyMine - An integrated database for Drosophila genomics
      Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
      InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.
      KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.
      Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
      Synonyms and Secondary IDs (17)
      Datasets (0)
      Study focus (0)
      Experimental Role
      Project
      Project Type
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      References (78)