FlyBase Gene Report: Dmel\MED6

MEDIATOR COMPLEX -: The Mediator (MED) complex serves as a hub for transcriptional signaling events. MED bridges transcriptional activators and repressors. This large multi-protein complex has a central core conserved from yeast to humans. The core is composed of three modules: head, middle, and tail. A separate module, the Cdk8 kinase module, composed of Cdk8, CycC, kto and skd, serves as a regulator. (Adapted from FBrf0226415 and FBrf0191850).

UniProt Contributed Function Data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for activated transcription of the MtnA, MtnB and MtnD genes.

(UniProt, Q8MSX2)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\MED6 or the JBrowse view of Dmel\MED6 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.49

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

MED6-RA

FBtr0082162

NM_143731

1819

249

MED6-RB

FBtr0082161

NM_169310

1758

249

MED6-RC

FBtr0301872

NM_001170106

1770

249

MED6-RD

FBtr0301873

NM_001170107

1814

249

MED6-RE

FBtr0339719

NM_001300335

1617

249

Additional Transcript Data and Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

MED6-PA

FBpp0081640

28.4

249

8.23

NP_651988

AAF54445

MED6-PB

FBpp0081639

28.4

249

8.23

NP_731403

AAN13445

MED6-PC

FBpp0291086

28.4

249

8.23

NP_001163577

ACZ94874

MED6-PD

FBpp0291087

28.4

249

8.23

NP_001163578

ACZ94875

MED6-PE

FBpp0308776

28.4

249

8.23

NP_001287264

AHN57263

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

249 aa isoforms: MED6-PA, MED6-PB, MED6-PC, MED6-PD, MED6-PE

Additional Polypeptide Data and Comments

Reported size (kDa)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the Mediator complex, which includes at least MED4, MED6, MED14, MED17, MED18, MED20, MED21, MED23, MED24, MED27, MED30 and MED31.

(UniProt, Q8MSX2)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\MED6 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Gene Ontology (16 terms)

Molecular Function (4 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

protein binding

inferred from physical interaction with UniProtKB:P91641

(assigned by UniProt )

(Gu et al., 2002)

inferred from physical interaction with UniProtKB:Q9V439

(assigned by UniProt )

(Gu et al., 2002)

inferred from physical interaction with UniProtKB:Q8IH24

(assigned by UniProt )

(Park et al., 2001)

inferred from physical interaction with UniProtKB:Q9VS38

(assigned by UniProt )

(Park et al., 2001)

inferred from physical interaction with UniProtKB:Q9VT57

(assigned by UniProt )

(Park et al., 2001)

inferred from physical interaction with UniProtKB:Q9W0P8

(assigned by UniProt )

(Park et al., 2001)

contributes_to transcription coactivator activity

inferred from direct assay

(Gu et al., 2002)

transcription coregulator activity

inferred from mutant phenotype

(assigned by UniProt )

(Gu et al., 2002, Park et al., 2001)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

RNA polymerase II transcription coactivator activity involved in preinitiation complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN000318543

(assigned by GO_Central )

(Gaudet et al., 2011)

transcription coactivator activity

inferred from sequence or structural similarity with UniProtKB:O75586

(Boube et al., 2000)

Biological Process (7 terms)

Terms Based on Experimental Evidence (5 terms)

CV Term

Evidence

References

defense response to Gram-negative bacterium

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

negative regulation of innate immune response

inferred from high throughput mutant phenotype

(Cronin et al., 2009)

negative regulation of neuroblast proliferation

inferred from mutant phenotype

(Homem et al., 2014)

regulation of transcription by RNA polymerase II

inferred from direct assay

(Gu et al., 2002)

transcription by RNA polymerase II

inferred from mutant phenotype

(assigned by UniProt )

(Gu et al., 2002, Park et al., 2001)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

positive regulation of transcription by RNA polymerase II

inferred from sequence or structural similarity with UniProtKB:O75586

(Boube et al., 2000)

regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000318543

(assigned by GO_Central )

(Gaudet et al., 2011)

RNA polymerase II preinitiation complex assembly

inferred from biological aspect of ancestor with PANTHER:PTN000318543

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (3 terms)

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Gim et al., 2001)

mediator complex

inferred from direct assay

(Gu et al., 2002)

inferred from mutant phenotype

(assigned by UniProt )

(Park et al., 2001)

nucleus

inferred from direct assay

(Gu et al., 2002, Gim et al., 2001)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

core mediator complex

inferred from biological aspect of ancestor with PANTHER:PTN000318543

(assigned by GO_Central )

(Gaudet et al., 2011)

mediator complex

inferred from biological aspect of ancestor with PANTHER:PTN000318543

(assigned by GO_Central )

(Gaudet et al., 2011)

nucleoplasm

inferred from sequence or structural similarity with UniProtKB:O75586

(Boube et al., 2000)

Expression Data

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

organism

Comment: expression strongest in adults

(Gim et al., 2001)

Additional Descriptive Data

MED6 transcript is expressed most strongly in adults, and is maternally deposited into eggs; transcript levels decrease during embryogenesis. Transcript expression increases during the early stages of pupariation.

(Gim et al., 2001)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

immunolocalization

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 17

organism | ubiquitous

• nucleus

(Gim et al., 2001)

adult stage | female

ovary

• nucleus

(Gim et al., 2001)

nurse cell

• nucleus

(Gim et al., 2001)

follicle cell

• nucleus

(Gim et al., 2001)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage

organism

• nucleus

(Gim et al., 2001)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

cytoplasm

inferred from direct assay

(Gim et al., 2001)

mediator complex

inferred from direct assay

(Gu et al., 2002)

inferred from mutant phenotype

(assigned by UniProt )

(Park et al., 2001)

nucleus

inferred from direct assay

(Gu et al., 2002, Gim et al., 2001)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\MED6 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\MED6

View exonic expression by tissue for Dmel\MED6

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024330

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0024330

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9473-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9473

Flygut - An atlas of the Drosophila adult midgut

FBgn0024330

Images

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs and Phenotypes

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Allele of MED6

Class

Mutagen

Associated Insertion

Stocks

Known lesion

MED6^EY05087

P-element activity

P{EPgy2}MED6^EY05087

Yes

MED6²⁶

amorphic allele - genetic evidence

ethyl methanesulfonate

Yes

MED6⁶⁴

amorphic allele - genetic evidence

ethyl methanesulfonate

Yes

MED6^unspecified

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of MED6

Allele of MED6

Mutagen

Associated Transgenic Construct

Stocks

in vitro construct - genomic fragment

P{MED6^+t10.4}

Transgenic constructs containing regulatory region of MED6

Deletions and Duplications ( 2 )

Disrupted in

Df(3R)by10

(Gim et al., 2001)

Df(3R)GB104

(Gim and Kim, 1998)

Not disrupted in

Df(3R)by10

(Gim and Kim, 1998)

Df(3R)GB104

(Gim et al., 2001)

Summary of Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

cell lethal | somatic clone

MED6²⁶

lethal

MED6⁶⁴

lethal - all die before end of larval stage (with Df(3R)by10)

lethal - all die before end of larval stage (with MED6²⁶)

MED6⁶⁴

lethal - all die before end of larval stage (with MED6⁶⁴)

MED6²⁶

lethal - all die before end of larval stage | recessive

MED6²⁶

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

MED6^GD4732

partially lethal - majority die, with Scer\GAL4^pnr-MD237

MED6^GD4732

viable

MED6^EY05087

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

MED6^GD4732

viable, with Scer\GAL4^tin.CΔ4

MED6^GD4732

Sterility

Allele

female sterile | germline clone | recessive

MED6²⁶

fertile

MED6^EY05087

Other Phenotypes

Allele

meiotic cell cycle defective, with Scer\GAL4^VP16.bam

MED6^KK101944

neuroanatomy defective | pupal stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

some die during pupal stage, with Scer\GAL4^pnr-MD237

MED6^GD4732

some die during third instar larval stage (with Df(3R)by10)

some die during third instar larval stage (with MED6²⁶)

MED6⁶⁴

some die during third instar larval stage (with MED6⁶⁴)

MED6²⁶

some die during third instar larval stage | recessive

MED6²⁶

visible, with Scer\GAL4^pnr-MD237

MED6^GD4732

Phenotype manifest in

Allele

chaeta | supernumerary, with Scer\GAL4^pnr-MD237

MED6^GD4732

eye | somatic clone

MED6²⁶

follicle cell | somatic clone

MED6²⁶

imaginal disc

MED6^unspecified

imaginal disc | somatic clone

MED6²⁶

mesothoracic tergum, with Scer\GAL4^pnr-MD237

MED6^GD4732

neuroblast | pupal stage, with Scer\GAL4^wor.PA, Scer\GAL80^ase.PN

spermatocyte, with Scer\GAL4^VP16.bam

MED6^KK101944

trichogen cell, with Scer\GAL4^pnr-MD237

MED6^GD4732

wing disc | second instar larval stage

MED6²⁶

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\MED6

14 of 15

Yes

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Med6

13 of 15

Yes

Rattus norvegicus (Norway rat) (1)

Rnor\Med6

13 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\med6

12 of 12

Yes

Danio rerio (Zebrafish) (1)

Drer\med6

14 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\mdt-6

14 of 15

Yes

Cele\sna-1

1 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\MED6

5 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (1)

Scer\MED6

11 of 15

Yes

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\pmc5

11 of 12

Yes

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190EZW )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\MED6

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21815

Drosophila persimilis

Dper\GL23233

Drosophila willistoni

Dwil\GK11125

Drosophila virilis

Dvir\GJ24525

Drosophila mojavensis

Dmoj\GI24883

Drosophila grimshawi

Dgri\GH14337

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG09150BXW )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17015737

Glossina morsitans

Tsetse fly

Gmor\GMOY003181

Mayetiola destructor

Hessian fly

Mdes\Mdes002681

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007825

Anopheles darlingi

American malaria mosquito

Adar\ADAC001055

Anopheles gambiae

Malaria mosquito

Agam\MED6

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ006056

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W0E1Z )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA004927

Danaus plexippus

Monarch butterfly

Dple\DPOGS202270-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL007645

Apis florea

Little honeybee

Aflo\2534497732

Apis mellifera

Western honey bee

Amel\MED6

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP18873

Bombus terrestris

Buff-tailed bumblebee

Bter\15216525

Linepithema humile

Argentine ant

Lhum\LH16336

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:00105c

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG002971

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_10564

Tribolium castaneum

Red flour beetle

Tcas\TC009982

Pediculus humanus

Human body louse

Phum\PHUM515150

Rhodnius prolixus

Kissing bug

Rpro\RPRC007824

Cimex lectularius

Bed bug

Clec\CLEC005416

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI001057

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_08861

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0E0D )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR010801

Ixodes scapularis

Black-legged tick

Isca\ISCW003442

Stegodyphus mimosarum

African social velvet spider

Smim\KFM83477.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur11g02660

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_26730

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G0MJA )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Med6

Ciona intestinalis

Vase tunicate

Cint\ENSCING00000014788

Gallus gallus

Domestic chicken

Ggal\LOC425109

Human Disease Model Data

FlyBase Human Disease Model Reports

Alleles Reported to Model Human Disease (Disease Ontology)

Download

Models ( 0 )

Allele

Disease

Evidence

References

Interactions ( 0 )

Allele

Disease

Interaction

References

Comments ( 0 )

Human Orthologs (via DIOPT v7.1)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

Complementation?

Transgene?

MED6; mediator complex subunit 6

14 of 15

602984

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please look at the Interaction Group reports for full details of the physical interactions

RNA-protein

Interacting group

Assay

References

MED6 - Imp

anti tag coimmunoprecipitation, quantitative reverse transcription pcr

(Yang et al., 2017)

protein-protein

Interacting group

Assay

References

MED6 - Bap60

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - CG17002

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - CG3008

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - CG34159

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Cdk8

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Cpsf6

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Larp4B

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED10

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED11

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED14

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED15

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED16

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED17

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED18

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED20

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED23

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED27

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED30

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED31

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED4

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED7

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - MED8

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Pa1

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Psi

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Ptip

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Ssdp

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - Usp10

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - boca

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - brm

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - dsh

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - ebi

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - enc

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - ix

experimental knowledge based

(Guruharsha et al., 2011)

MED6 - mor

experimental knowledge based

(Guruharsha et al., 2011)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the Mediator complex, which includes at least MED4, MED6, MED14, MED17, MED18, MED20, MED21, MED23, MED24, MED27, MED30 and MED31.

(UniProt, Q8MSX2 )

Linkouts

BioGRID - A database of protein and genetic interactions.

69231

DroID - A comprehensive database of gene and protein interactions.

FBgn0024330

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44728

Pathways

Gene Group - Pathway Membership (FlyBase)

External Data

Linkouts

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Generic Transcription Pathway

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-49

Cytogenetic map

85E8-85E8

Sequence location

3R:9,766,031..9,768,984 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

85E8-85E8

Limits computationally determined from genome sequence between P{EP}Crc^EP3006 and P{lacW}s2681

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

85E11-85E13

(determined by in situ hybridisation)

(Gim and Kim, 1998)

MED6 is located between the distal breakpoints of Df(3R)by10 and Df(3R)GB104.

(Gim and Kim, 1998)

Experimentally Determined Recombination Data

Location

3-49

(FlyBase, 2016)

3-46.2

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

33743

y¹ sc^* v¹; P{TRiP.HMS01081}attP2/TM3, Sb¹

16634

y¹ w^67c23; P{EPgy2}MED6^EY05087 CG8481^EY05087

VDRC

v108942

P{KK101944}VIE-260B

v15339

w¹¹¹⁸; P{GD4732}v15339/TM3

Genomic Clones (11)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (78)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0024330

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0024330

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44728

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Gim et al., 2001)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: Trap32 CG9473

(Boube et al., 2000)

Source for identity of: MED6 Med6

(Bourbon et al., 2004)

Source for database merge of

Source for merge of: CG9473 anon- EST:Posey279

(Bayraktaroglu, 2000.11.2)

Source for merge of: Med6 BcDNA:LD15729

(FlyBase, 1996-)

Additional comments

Source for merge of Med6 BcDNA:LD15729 was a shared cDNA ( date:020730 ).

(FlyBase, 1996-)

Other Comments

dsRNA has been made from templates generated with primers directed against this gene.

(Marr et al., 2006)

A homolog of yeast MED6 is cloned in Drosophila using degenerate PCRs based on the conserved MED6 sequences, and its genomic and cDNA structures determined.

(Gim and Kim, 1998)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 52 )

Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

44728

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

Q8MSX2

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

E1JIH5

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024330

InterPro - A database of protein families, domains and functional sites

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Generic Transcription Pathway

Linkouts

BioGRID - A database of protein and genetic interactions.

69231

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center cDNA clones

FBgn0024330

DPiM - Drosophila Protein interaction map

FBgn0024330

DroID - A comprehensive database of gene and protein interactions.

FBgn0024330

DRSC - Results frm RNAi screens

FBgn0024330

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0024330

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9473-RB

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9473

Flygut - An atlas of the Drosophila adult midgut

FBgn0024330

FlyMine - An integrated database for Drosophila genomics

FBgn0024330

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

44728

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC009982

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

44728

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9473

modMine - A data warehouse for the modENCODE project

FBgn0024330

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BcDNA:LD15729

CG9473

(Dahlberg et al., 2015, Hosono et al., 2015, Lu and Fuller, 2015, Ni et al., 2010.12.1, Keleman et al., 2009.8.5, Gim et al., 2001)

MED6

(Toku et al., 2011, Cronin et al., 2009, Marr et al., 2006)

Med6

(Yang et al., 2017, Lu and Fuller, 2015, Kim and Lis, 2005, Bourbon et al., 2004, Guglielmi et al., 2004, Kim et al., 2004, Orian et al., 2003, Boube et al., 2002, FlyBase, 1996-)

Trap32

anon-EST:Posey279

dMED6

(Park et al., 2003, Gu et al., 2002)

dMed6

(Gim et al., 2001, Gim and Kim, 1998)

dTRAP32

(Boube et al., 2000)

med6

(Homem et al., 2014)

p35

(Park et al., 2001)

Name Synonyms

Mediator

(Park et al., 2001)

Mediator complex subunit 6

(Bourbon et al., 2004)

Secondary FlyBase IDs

FBgn0027700
FBgn0037748
FBgn0063159

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (42)

The following external sites may use different assemblies or annotations than FlyBase.

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