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Gene Dmel\MED6

General Information
Symbol	Dmel\MED6	Species	D. melanogaster
Name	Mediator complex subunit 6	Annotation symbol	CG9473
Feature type	protein_coding_gene	FlyBase ID	FBgn0024330
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	dMED6
Genomic Location
Chromosome (arm)	3R	Recombination map
Cytogenetic map	85E8-85E8	Sequence location	3R:5,591,753..5,594,706 [-]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Mediator complex subunit 6 is referred to in FlyBase by the symbol Dmel\MED6 (CG9473, FBgn0024330). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: protein binding; RNA polymerase II transcription cofactor activity; transcription coactivator activity. There is experimental evidence that it is involved in the biological process: lateral inhibition; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter. 11 alleles are reported. The phenotypes of these alleles are annotated with: trichogen cell; mesothoracic tergum; imaginal disc. It has 5 annotated transcripts and 5 annotated polypeptides. Protein features are: Mediator complex, subunit Med6; Mediator complex, subunit Med6, metazoa/plant. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, adult testis. Comments on Affy2 ProbeSet: ProbeSet 1641537_s_at completely aligns to an exonic region common to each of the 4 FlyBase-annotated transcript isoforms of MED6. Gene sequence location is 3R:5591753..5594706.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 85E8-85E8   Limits computationally determined from genome sequence between P{EP}CrcEP3006 and P{lacW}s2681
Experimentally Determined Cytological Location
Cytogenetic map Notes References 85E11-85E13   MED6 is located between the distal breakpoints of Df(3R)by10 and Df(3R)GB104. (determined by in situ hybridisation)   (Gim and Kim, 1998)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\MED6 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.49 Low-frequency RNA-Seq exon junction(s) not annotated.
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) MED6-RA FBtr0082162  NM_143731   1819   249 MED6-RB FBtr0082161  NM_169310   1758   249 MED6-RC FBtr0301872  NM_001170106   1770   249 MED6-RD FBtr0301873  NM_001170107   1814   249 MED6-RE FBtr0339719     1617   249
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein MED6-PA   FBpp0081640   28.4   249   8.23     NP_651988   AAF54445 MED6-PB   FBpp0081639   28.4   249   8.23     NP_731403   AAN13445 MED6-PC   FBpp0291086   28.4   249   8.23     NP_001163577   ACZ94874 MED6-PD   FBpp0291087   28.4   249   8.23     NP_001163578   ACZ94875 MED6-PE   FBpp0308776   28.4   249   8.23
Additional Polypeptide Data & Comments
Reported size (kDa)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med6 (IPR007018) Mediator complex, subunit Med6, metazoa/plant (IPR016820)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263380   AA391312   AA438890   AA440829   AA536467   AE014297 AAF54445   AE014297 AAN13445   AE014297 ACZ94874   AE014297 ACZ94875   AF171864   AI063274   AI257616   AI259497   AI456437   AI456856   AW942376   AY118517 AAM49886   BI213644   BI236516   BI241569   BI352093   BI354891   BI356473   BI639442   BT001633 AAN71388   CK132048   CL527783   CO154182   CO190550   CO310271   CO344481   EC069544   EC225082   EC226381   EC233469   EC256307   EC266435   GH753799   GH754992   GH755453   GH770439   GH779059   GH785638   GH786809   GH789562   GH790753   GH794298   GH795048   GH795758   GH804572   GH805302   GH805595   GH805994   GH817416   GH819441   GH831198   GH832477   GH832919   GH833180   GH842539   GH848070   GH848745   GH849091   GH861939   GH862021   GH872348   GH873267   GH873879   GH874101   GH884366   GH885411   GH887189   GH898232   GH907082   GH909928   GH918455   GH919201   GH925069   GH931088   GH932351   GH932795   GH936662   GH939021   GH939653   GH942721   GH943132   GH944712   GH945235   GH946672   GH948275   GH950619   GH953108   GH954302   GH956200   GH962215   GH963077   GH968676   GH970019   GH970085   GH972793   GH973797   GH976079   GH977634   GH982372   GH983590
UniProtKB/Swiss-Prot	Q8MSX2
UniProtKB/TrEMBL	E1JIH5
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   organism | faint & ubiquitous   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) embryonic stage 9 -- 10   anterior endoderm   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) organism | ubiquitous   (Fisher et al., 2012) posterior endoderm   (Fisher et al., 2012) embryonic stage 11 -- 12   anterior midgut primordium   (Fisher et al., 2012) central brain primordium   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) posterior midgut primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic/larval midgut   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage -- adult stage   organism   (Gim et al., 2001) Comment:expression strongest in adults
Additional Descriptive Data
	MED6 transcript is expressed most strongly in adults, and is maternally deposited into eggs; transcript levels decrease during embryogenesis. Transcript expression increases during the early stages of pupariation. (Gim et al., 2001)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
immunolocalization Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 17   organism | ubiquitous   • nucleus (Gim et al., 2001) adult stage | female   ovary   • nucleus (Gim et al., 2001) nurse cell   • nucleus (Gim et al., 2001) follicle cell   • nucleus (Gim et al., 2001) western blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage   organism   • nucleus (Gim et al., 2001)
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
cytoplasm inferred from direct assay (Gim et al., 2001) mediator complex inferred from direct assay (Gu et al., 2002) inferred from mutant phenotype (assigned by UniProtKB) (Park et al., 2001) nucleus inferred from direct assay (Gim et al., 2001, Gu et al., 2002)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: larval/adult central nervous system, larval salivary gland, larval trachea, adult ovary, adult testis. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    245.15 Larval Midgut    48.6 Larval Hindgut    82.6 Larval Malpighian Tubules    83.4 Larval Fat Body    66.1 Larval Salivary Gland    104.9 Larval Trachea    106.975 Larval Carcass    83.425 Adult Head    57.2 Adult Eye    84.1 Adult Brain    138 Adult Thoracic-Abdominal Ganglion    122.6 Adult Crop    75 Adult Midgut    44.9 Adult Hindgut    57.3 Adult Malpighian Tubules    96.3 Adult Fat Body    39.8 Adult Salivary Gland    76.5 Adult Heart    43.9 Adult VirginFemale Spermatheca    43.8 Adult InseminatedFemale Spermatheca    51.4 Adult Ovary    204.7 Adult Testis    112.2 Adult Male Accessory Gland    95.5 Adult Carcass    47.6 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    57.2   NA  Eye    84.1   NA  Brain    138   245.15  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    122.6   NA  Crop    75   48.6  Midgut    44.9   82.6  Hindgut    57.3   83.4  Malpighian Tubules    96.3   66.1  Fat Body    39.8   104.9  Salivary Gland    76.5   NA  Heart    43.9   106.975  Trachea    NA   NA  VirginFemale Spermatheca    43.8   NA  InseminatedFemale Spermatheca    51.4   NA  Ovary    204.7   NA  Testis    112.2   NA  Male Accessory Gland    95.5   83.425  Carcass    47.6 FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    29 ovary, mated 4-day female    29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  (29) central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female  (29) ovary, mated 4-day female  (29) testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    29 ovary, mated 4-day female    29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    29 ovary, mated 4-day female    29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    29 ovary, mated 4-day female    29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering  29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female  29 ovary, mated 4-day female  29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    29 ovary, mated 4-day female    29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    29 central nervous system, larvae L3    25 central nervous system, pupae P8    11 head, virgin 1-day female    8 head, virgin 4-day female    5 head, virgin 20-day female    4 head, mated 1-day female    6 head, mated 4-day female    5 head, mated 20-day female    4 head, mated 1-day male    10 head, mated 4-day male    10 head, mated 20-day male    10 salivary gland, larvae L3 wandering    4 salivary gland, white prepupae    12 digestive system, larvae L3 wandering    10 digestive system, 1-day adult    5 digestive system, 4-day adult    4 digestive system, 20-day adult    6 fat body, larvae L3 wandering    3 fat body, white prepupae    8 fat body, pupae P8    9 carcass, larvae L3 wandering    17 carcass, 1-day adult    5 carcass, 4-day adult    5 carcass, 20-day adult    5 ovary, virgin 4-day female    29 ovary, mated 4-day female    29 testis, mated 4-day male    13 accessory gland, mated 4-day male    7 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    34 embryo 04-06hr    30 embryo 06-08hr    28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr  (34) embryo 04-06hr  (30) embryo 06-08hr  (28) embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    34 embryo 04-06hr    30 embryo 06-08hr    28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    34 embryo 04-06hr    30 embryo 06-08hr    28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    34 embryo 04-06hr    30 embryo 06-08hr    28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (33) embryo 02-04hr  (34) embryo 04-06hr  30 embryo 06-08hr  28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    34 embryo 04-06hr    30 embryo 06-08hr    28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    33 embryo 02-04hr    34 embryo 04-06hr    30 embryo 06-08hr    28 embryo 08-10hr    25 embryo 10-12hr    19 embryo 12-14hr    15 embryo 14-16hr    14 embryo 16-18hr    10 embryo 18-20hr    8 embryo 20-22hr    10 embryo 22-24hr    10 larva L1    9 larva L2    7 larva L3 12hr old    5 larva L3 puffstage 1-2    7 larva L3 puffstage 3-6    10 larva L3 puffstage 7-9    13 white prepupae new    12 white prepupae 12hr    15 white prepupae 24hr    17 pupae 2d postWPP    10 pupae 3d postWPP    7 pupae 4d postWPP    9 adult male 01day    9 adult male 05day    10 adult male 30day    11 adult female 01day    14 adult female 05day    20 adult female 30day    20 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1    31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  (32) Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1  (31) embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9  (28) wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1    31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1    31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1    31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+  32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1  31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9  28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1    31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    32 Schneider line 2 Sg4    15 embryonic 1182-4H    18 embryonic GM2    21 embryonic Kc167    13 embryonic S1    31 embryonic S3    20 leg disc CME L1    21 wing disc CME-W2    12 wing disc ML-DmD8    19 wing disc ML-DmD9    28 wing disc ML-DmD16-c3    10 wing disc ML-DmD21    18 wing disc ML-DmD32    20 haltere disc ML-DmD17-c3    23 eye-antennal disc ML-DmD11    22 antennal disc ML-DmD20-c5    16 mixed discs ML-DmD4-c1    19 CNS ML-DmBG1-c1    18 CNS ML-DmBG2-c2    23 tumorous blood cells mbn2    14 ovary fGS/OSS    19 ovary OSC    17 ovary OSS    15 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    14 cold shock, 4-day adult    10 heat shock, 4-day adult    11 Cadmium 50 mM 6 hrs, larvae L3    9 Cadmium 50 mM 12 hrs, larvae L3    9 Cadmium 50 mM 48 hrs, 4-day adult    13 Cadmium 100 mM 48 hrs, 4-day adult    23 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    9 Zinc 5 mM 12 hrs, larvae L3    9 Zinc 4.5 mM 48 hrs, 4-day adult    8 Ethanol 2.5% 3 hrs, larvae L3    24 Ethanol 5% 3 hrs, larvae L3    5 Ethanol 10% 3 hrs, larvae L3    8 Caffeine 1.5 mg/ml 4 hrs, larvae L3    7 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    24 Caffeine 25 mg/ml 48 hrs, 4-day adult    10 Paraquat 5 mM 48 hrs, 4-day adult    19 Paraquat 10 mM 48 hrs, 4-day adult    18 Rotenone 2 μg 12 hrs, larvae L3    9 Rotenone 8 μg 12 hrs, larvae L3    8 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_17 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD15729 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024330 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9473-RB
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele cell lethal | somatic clone MED626 lethal MED664 lethal | recessive | third instar larval stage MED626 lethal | third instar larval stage (with Df(3R)by10) MED626 MED664 lethal | third instar larval stage (with MED626) MED664 lethal | third instar larval stage (with MED664) MED626 partially lethal - majority die | pupal stage, with Scer\GAL4pnr-MD237 MED6GD4732 viable MED6EY05087 Sterility Allele female sterile | germline clone | recessive MED626 fertile MED6EY05087 Other Phenotypes Allele visible, with Scer\GAL4pnr-MD237 MED6GD4732 Phenotype manifest in Allele chaeta | supernumerary, with Scer\GAL4pnr-MD237 MED6GD4732 eye | somatic clone MED626 follicle cell | somatic clone MED626 imaginal disc MED6unspecified imaginal disc | somatic clone MED626 mesothoracic tergum, with Scer\GAL4pnr-MD237 MED6GD4732 trichogen cell, with Scer\GAL4pnr-MD237 MED6GD4732 wing disc | second instar larval stage MED626
Classical Alleles ( 4 )
For All Classical Alleles Show
Allele of MED6 Class Mutagen Stocks Known lesion MED6EY05087 P-element activity 1 -- MED626 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes MED664 amorphic allele - genetic evidence ethyl methanesulfonate 0 Yes MED6unspecified 0 --
Alleles Carried on Transgenic Constructs ( 7 )
For All Alleles Carried on Transgenic Constructs Show
Allele of MED6 Class Mutagen Stocks Known lesion MED6GD4732 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes MED6HMS01081 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes MED6+t10.4 in vitro construct - genomic fragment 0 Yes MED6a.cKa in vitro construct - RNAi 0 Yes MED6cKa in vitro construct - RNAi 0 Yes MED6KK101944 in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes MED6T:Ivir\HA1 in vitro construct - regulatory fusion in vitro construct - coding region fusion 0 Yes
Aneuploid Aberrations
Disrupted in	Df(3R)by10 (Gim et al., 2001) Df(3R)GB104 (Gim and Kim, 1998)
Not disrupted in	Df(3R)by10 (Gim and Kim, 1998) Df(3R)GB104 (Gim et al., 2001)
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4732} NA P{KK101944} NA P{TRiP.HMS01081} NA characterization construct P{MED6+t10.4} NA
Insertions
	Type of insertions Name Expression data insertion of mobile activating element P{EPgy2}MED6EY05087 NA
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 9 terms )
Molecular Function
CV term Evidence References
protein binding inferred from physical interaction with UniProtKB:Q8IH24 (assigned by UniProtKB) (Park et al., 2001) inferred from physical interaction with UniProtKB:Q9VS38 (assigned by UniProtKB) (Park et al., 2001) inferred from physical interaction with UniProtKB:Q9VT57 (assigned by UniProtKB) (Park et al., 2001) inferred from physical interaction with UniProtKB:Q9W0P8 (assigned by UniProtKB) (Park et al., 2001) RNA polymerase II transcription cofactor activity inferred from mutant phenotype (assigned by UniProtKB) (Park et al., 2001) contributes_to transcription coactivator activity inferred from direct assay (Gu et al., 2002)
Biological Process
CV term Evidence References
lateral inhibition inferred from mutant phenotype (Mummery-Widmer et al., 2009) regulation of transcription from RNA polymerase II promoter inferred from direct assay (Gu et al., 2002) transcription from RNA polymerase II promoter inferred from mutant phenotype (assigned by UniProtKB) (Park et al., 2001)
Cellular Component
CV term Evidence References
cytoplasm inferred from direct assay (Gim et al., 2001) mediator complex inferred from direct assay (Gu et al., 2002) inferred from mutant phenotype (assigned by UniProtKB) (Park et al., 2001) nucleus inferred from direct assay (Gim et al., 2001, Gu et al., 2002)
Terms Based on Predictions or Assertions ( 5 terms )
Molecular Function
CV term Evidence References
RNA polymerase II transcription cofactor activity inferred from sequence or structural similarity (Gim and Kim, 1998, Boube et al., 2000) inferred from sequence or structural similarity with EMBL:AF074723 (FlyBase, 1992-)
Biological Process
CV term Evidence References
transcription from RNA polymerase II promoter inferred from sequence or structural similarity (Gim and Kim, 1998) inferred from sequence or structural similarity with EMBL:AF074723 (FlyBase, 1992-) transcription initiation from RNA polymerase II promoter inferred from sequence or structural similarity (Boube et al., 2000)
Cellular Component
CV term Evidence References
mediator complex inferred from sequence or structural similarity (Gim and Kim, 1998, Boube et al., 2000) inferred from sequence or structural similarity with EMBL:AF074723 (FlyBase, 1992-) nucleus inferred from sequence or structural similarity with EMBL:AF074723 (FlyBase, 1992-)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
MED6 - Atg7 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Bap60 experimental knowledge based (Guruharsha et al., 2011) MED6 - Bx anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG11107 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG11505 experimental knowledge based (Guruharsha et al., 2011) MED6 - CG12129 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG12493 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG15625 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG17002 experimental knowledge based (Guruharsha et al., 2011) MED6 - CG17118 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG17454 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG1785 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG3008 experimental knowledge based (Guruharsha et al., 2011) MED6 - CG30491 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - CG32479 experimental knowledge based (Guruharsha et al., 2011) MED6 - CG34159 experimental knowledge based (Guruharsha et al., 2011) MED6 - CG7185 experimental knowledge based (Guruharsha et al., 2011) MED6 - CG7542 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Cdk8 experimental knowledge based (Guruharsha et al., 2011) MED6 - Chi anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Df31 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - LKR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - MED10 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED11 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) MED6 - MED14 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED15 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED16 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED17 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED18 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED20 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED23 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED27 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED30 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED31 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED4 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED7 experimental knowledge based (Guruharsha et al., 2011) MED6 - MED8 experimental knowledge based (Guruharsha et al., 2011) MED6 - Pa1 experimental knowledge based (Guruharsha et al., 2011) MED6 - Pgam5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Prp3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Psi experimental knowledge based (Guruharsha et al., 2011) MED6 - Rbp1-like anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Rrp45 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Saf-B anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - SmD2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - Ssdp experimental knowledge based (Guruharsha et al., 2011) MED6 - TfIIFβ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - TfIIS anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - U3-55K anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - boca experimental knowledge based (Guruharsha et al., 2011) MED6 - brm experimental knowledge based (Guruharsha et al., 2011) MED6 - dsh experimental knowledge based (Guruharsha et al., 2011) MED6 - ebi experimental knowledge based (Guruharsha et al., 2011) MED6 - enc experimental knowledge based (Guruharsha et al., 2011) MED6 - fan anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - fh anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - ix experimental knowledge based (Guruharsha et al., 2011) MED6 - mRpS24 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - mor experimental knowledge based (Guruharsha et al., 2011) MED6 - p53 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) MED6 - ptip experimental knowledge based (Guruharsha et al., 2011) MED6 - spoon anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions MED6 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 69231 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024330 DroID - A comprehensive database of gene and protein interactions. • FBgn0024330 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44728
Orthologs
OrthoDB Orthologs (38) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG6JQ4HZ Dipteran inclusive ortholog search EOG6VT66B Insect inclusive ortholog search EOG663Z83 Arthropod inclusive ortholog search EOG6SQVCR Metazoa inclusive ortholog search EOG61ZT5C
Orthologs in Drosophila Species (EOG6JQ4HZ)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\MED6     Drosophila sechellia   Dsec\GM26214 Y   Drosophila erecta   Dere\GG17328 Y   Drosophila yakuba   Dyak\GE24733 Y   Drosophila ananassae   Dana\GF23191 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA21815 Y   Drosophila persimilis   Dper\GL23233 Y   Drosophila willistoni   Dwil\GK11125 Y   Drosophila virilis   Dvir\GJ24525 Y   Drosophila mojavensis   Dmoj\GI24883 Y   Drosophila grimshawi   Dgri\GH14337 Y
Orthologs in non-Drosophila Dipterans (EOG6VT66B)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL007825   Anopheles gambiae Malaria mosquito  Agam\MED6   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ006056
Orthologs in non-Dipteran Insects (EOG663Z83)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\MED6   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG002971   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11274   Atta cephalotes Leafcutter ant  Acep\ACEP14265   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19655   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15699   Linepithema humile Argentine ant  Lhum\LH16336   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17556   Solenopsis invicta Red fire ant  Sinv\SINV11030   Acyrthosiphon pisum Pea aphid  Apim\ACYPI001057   Bombyx mori Silkmoth  Bmor\BGIBMGA004927   Pediculus humanus Human body louse  Phum\PHUM515150   Tribolium castaneum Red flour beetle  Tcas\TC009982
Orthologs in non-Insect Arthropods (EOG6SQVCR)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\JGI_V11_26730   Ixodes scapularis Deer tick  Isca\ISCW003442
Orthologs in non-Arthropod Metazoa (EOG61ZT5C)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\mdt-6   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_001146gn   Danio rerio Zebrafish  Drer\med6   Xenopus tropicalis Western clawed frog  Xtro\med6   Gallus gallus Domestic chicken  Ggal\LOC425109   Gallus gallus Domestic chicken  Ggal\ENSGALG00000000034   Mus musculus House mouse  Mmus\Med6   Rattus norvegicus Norway rat  Rnor\Med6   Homo sapiens Human  Hsap\MED6
Human Orthologs (1)
Gene OMIM HGNC Hsap\MED6
AAA Orthologs (10) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila sechellia Dsec\GM26214 Drosophila erecta Dere\GG17328 Drosophila yakuba Dyak\GE24733 Drosophila ananassae Dana\GF23191 Drosophila pseudoobscura pseudoobscura Dpse\GA21815 Drosophila persimilis Dper\GL23233 Drosophila willistoni Dwil\GK11125 Drosophila virilis Dvir\GJ24525 Drosophila mojavensis Dmoj\GI24883 Drosophila grimshawi Dgri\GH14337
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	33743 y1 sc* v1; P{TRiP.HMS01081}attP2/TM3, Sb1 16634 y1 w67c23; P{EPgy2}MED6EY05087 CG8481EY05087
VDRC	v15337 w1118; P{GD4732}v15337/TM3 v15339 w1118; P{GD4732}v15339/TM3
Genomic Clones ( 2 )
	BACR23O04 BACR30F01 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 98 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LD15729 RE38674
Other clones	AF171864 DPiM_LD15729
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	GM17606 GM28719 LD06223 LD09827 LD13271 LD17085 LD33712 LD36458 LD37008 LP02921 LP06029 LP13268 RE19085 RE32580 RE42640 SD22018
Other clones	34513 98188 147591 214315.38 523227 CK01.61C.G7 EK048528 EK220939 EN03653 EP19345 RP003006891 RP003017918 RP003019983 RP003093677 RP003119257 RP003155513 RP003158854 RP003164542 RP003175998 RP003193779 RP003209463 RP003212619 RP003213305 RP003224808 RP003257122 RP003275558 RP003286167 RP003295491 RP003305920 RP003307310 RP003313756 RP003360602 RP003383121 RP003389614 RP003393295 RP003406903 RP003414625 RP003427164 RP003462258 RP003464186 RP003485058 RP003487410 RP003490329 RP003491548 RP003500857 RP003511202 RP003541122 RP003549251 RP003564984 RP003565963 RP003568366 RP003596493 RP003621840 RP003641890 RP003650980 RP003657528 RP003679574 RP004083872 RP004115814 RP004134588 RP004172546 RP004198417 RP004207358 RP004217608 RP004223135 RP004233145 RP004237920 RP004253400 RP004256598 RP004285237 RP004327521 RP004375535 RP004391999 RP004433097 RP004433311 RP004451902 RP004518182 RP004527602
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024330 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44728
Antibody Information
	polyclonal (Gim et al., 2001)
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: MED6 Med6 (Bourbon et al., 2004) Source for identity of: Trap32 CG9473 (Boube et al., 2000)
Source for database merge of	Source for merge of: CG9473 anon-EST:Posey279 (Bayraktaroglu, 2000.11.2) Source for merge of: Med6 BcDNA:LD15729 (FlyBase, 1996-)
Additional comments	Source for merge of Med6 BcDNA:LD15729 was a shared cDNA (date:020730). (FlyBase, 1996-)
Other Comments
	dsRNA has been made from templates generated with primers directed against this gene. (Marr et al., 2006) A homolog of yeast MED6 is cloned in Drosophila using degenerate PCRs based on the conserved MED6 sequences, and its genomic and cDNA structures determined. (Gim and Kim, 1998)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_143731 NM_169310 NM_001170106 NM_001170107 RefSeq (Proteins) • NP_651988 NP_731403 NP_001163577 NP_001163578 DDBJ / EMBL / GenBank • AA440829 AAF54445 AAM49886 AAN13445 AAN71388 ACZ94874 ACZ94875 AF171864 AW942376 AY118517 BI241569 BT001633 CL527783 Entrez Gene - A searchable database of RefSeq genes. • 44728 UniProtKB/Swiss-Prot • Q8MSX2 UniProtKB/TrEMBL • E1JIH5
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Mediator complex, subunit Med6 (IPR007018) Mediator complex, subunit Med6, metazoa/plant (IPR016820) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD15729
Linkouts
	BioGRID - A database of protein and genetic interactions • 69231 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024330 DroID - A comprehensive database of gene and protein interactions. • FBgn0024330 DRSC - Results from RNAi screens. • FBgn0024330 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024330 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG9473-RB FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024330 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 44728 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 44728 modMine - Data generated by the modENCODE project. • FBgn0024330
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	anon-EST:Posey279   BcDNA:LD15729   CG9473 (Keleman et al., 2009.8.5, Gim et al., 2001, Ni et al., 2010.12.1) dMED6 (Gu et al., 2002, Park et al., 2003) dMed6 (Gim and Kim, 1998, Gim et al., 2001) dTRAP32 (Boube et al., 2000) Med6 (Kim et al., 2004, Bourbon et al., 2004, Orian et al., 2003, Boube et al., 2002, FlyBase, 1996-, Kim and Lis, 2005, Guglielmi et al., 2004) MED6 (Marr et al., 2006, Toku et al., 2011, Cronin et al., 2009) p35 (Park et al., 2001) Trap32
Name Synonym	Mediator (Park et al., 2001) Mediator complex subunit 6 (Bourbon et al., 2004)
Secondary FlyBase IDs
	FBgn0027700 FBgn0037748 FBgn0063159
References ( 35 )
Generate a list of	All references relating to this gene ( 35 )
List References by type	Research paper  ( 13 ) Supplementary material  ( 5 ) Review  ( 2 ) Personal communication to FlyBase  ( 8 ) Abstract  ( 1 ) FlyBase analysis  ( 2 ) Letter  ( 1 ) Website  ( 1 ) Curated genome annotation  ( 2 )
Recent research papers ( 2 )
	Baradaran-Heravi et al., 2012, Hum. Mol. Genet. 21(11): 2572--2587 Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. [FBrf0218299] Toku et al., 2011, Comput. Biol. Chem. 35(5): 282--292 Reconstruction and crosstalk of protein-protein interaction networks of Wnt and Hedgehog signaling in Drosophila melanogaster. [FBrf0216393] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...