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Gene Dmel\Mcm3

General Information
Symbol	Dmel\Mcm3	Species	D. melanogaster
Name	Minichromosome maintenance 3	Annotation symbol	CG4206
Feature type	protein_coding_gene	FlyBase ID	FBgn0024332
Gene Model Status	Current	Stock availability	4 publicly available
Also Known As	DmMcm3
Genomic Location
Chromosome (arm)	X	Recombination map
Cytogenetic map	4F5-4F5	Sequence location	X:5,217,888..5,220,771 [+]
Genomic Maps Select View:Gene Models/EvidenceExpression/RegulationMegaViewGene Disruptions, Stocks and Reagents Beta	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	The gene Minichromosome maintenance 3 is referred to in FlyBase by the symbol Dmel\Mcm3 (CG4206, FBgn0024332). It is a protein_coding_gene from Drosophila melanogaster. There is experimental evidence that it has the molecular function: 3'-5' DNA helicase activity. There is experimental evidence that it is involved in the biological process: mitotic G2 DNA damage checkpoint; neurogenesis. 5 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides. Protein features are: AAA+ ATPase domain; DNA replication licensing factor Mcm3; Mini-chromosome maintenance, DNA-dependent ATPase; Mini-chromosome maintenance, conserved site; Nucleic acid-binding, OB-fold. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, larval trachea. Comments on Affy2 ProbeSet: ProbeSet 1635234_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of Mcm3. Gene sequence location is X:5217888..5220771.
User Contributed Data
	Beta version, feedback welcome
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 4F5-4F5   Limits computationally determined from genome sequence between P{EP}EP425 and P{EP}CG3249EP1400
Experimentally Determined Cytological Location
Cytogenetic map Notes References 4F9-5A2     (Feger, 1999.2.1) 4F-4F   (determined by in situ hybridisation)   (Ohno et al., 1998) 4F9-5A2   Mcm3 maps to 4F9-10/5A1-2. (determined by in situ hybridisation)   (Feger, 1999)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Gene Model & Products
Please see the GBrowse view of Dmel\Mcm3 for information on other features To submit a correction to a gene model please use the Contact FlyBase form
Comments on Gene Model
	Gene model reviewed during 5.50
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) Mcm3-RA FBtr0070762  NM_078493   2747   819 Mcm3-RB FBtr0340476     2744   818
Additional Transcript Data & Comments
Reported size (kB)	3.0 (northern blot) (Ohno et al., 1998)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kDa) Length (aa) Theoretical pI RefSeq ID GenBank protein Mcm3-PA   FBpp0070729   90.9   819   6.35     NP_511048   AAF46023 Mcm3-PB   FBpp0309417   90.8   818   6.26
Additional Polypeptide Data & Comments
Reported size (kDa)	819 (aa); 90.0 (kD predicted) (Feger, 1999) 819 (aa) (Ohno et al., 1998)
Comments
External Data
Linkouts
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Mini-chromosome maintenance, conserved site (IPR018525) Mini-chromosome maintenance, DNA-dependent ATPase (IPR001208) AAA+ ATPase domain (IPR003593) DNA replication licensing factor Mcm3 (IPR008046) Nucleic acid-binding, OB-fold (IPR012340) P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AA263937   AA438455   AA440724   AA942168   AA950813   AE014298 AAF46023   AB010107 BAA34731   AF124745 AAD32859   AI455619   AI456630   AI530865   AI542936   AW941632   BG635734   BP542847   BP542848   BT046156   CO154241   EC262799   EL870319   EL870320   EL886379   EL886707   EV593003   GH943942   GH947000   GH947242   GH951636   GH960313   GH961075   GH964131   GH969838   GH970548   GH973354   GH974773   GH979248   GH980592   GH981268   U83492 AAC47651
UniProtKB/Swiss-Prot	Q9XYU1
UniProtKB/TrEMBL	Q7JNE2
Mapped Features
Mapped Features have been reorganized, please see this article for details. Additional mapped features and mutations can be found on GBrowse or related reports. Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
Transcript Expression
in situ Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 3   organism   (Fisher et al., 2012) Comment:maternally deposited embryonic stage 4 -- 6   organism | faint & ubiquitous   (Fisher et al., 2012) embryonic stage 7 -- 8   head mesoderm   (Fisher et al., 2012) organism | faint & ubiquitous   (Fisher et al., 2012) trunk mesoderm   (Fisher et al., 2012) embryonic stage 9 -- 10   antennal primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium central brain primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium visual primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium dorsal head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium lateral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral head epidermis primordium   (Fisher et al., 2012) Comment:reported as procephalic ectoderm primordium ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 11 -- 12   presumptive embryonic/larval central nervous system   (Feger, 1999) central brain primordium   (Fisher et al., 2012) embryonic ganglion mother cell   (Fisher et al., 2012) embryonic neuroblast of ventral nerve cord primordium   (Fisher et al., 2012) lateral cord glial cell   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) procephalic neuroblast   (Fisher et al., 2012) ventral nerve cord primordium   (Fisher et al., 2012) embryonic stage 13 -- 16   embryonic brain   (Fisher et al., 2012) embryonic central brain neuron   (Fisher et al., 2012) embryonic/larval nervous system   (Fisher et al., 2012) lateral cord neuron   (Fisher et al., 2012) lateral cord surface glial cell   (Fisher et al., 2012) ventral nerve cord   (Fisher et al., 2012) northern blot Stage Tissue/Position (including subcellular localization) Reference embryonic stage 1 -- 9     (Ohno et al., 1998) Comment:reference states 0-4 hr AEL adult stage | female     (Ohno et al., 1998) unfertilized egg stage   unfertilized egg   (Ohno et al., 1998)
Additional Descriptive Data
	Mcm3 transcript levels are high in unfertilized eggs, slightly higher in 2-4 hour embryos, and are lower after 4 hours of embryogenesis. Levels are low in other developmental stages, with the exception of adult females. (Ohno et al., 1998) Mcm3 transcript expression pattern resembles that of DNA replication. Mcm3 transcript is detected at high levels in the central nervous system and at very low levels in the gut of stage 11-12 embryos. (Feger, 1999)
Marker for
Subcellular Localization
CV Term
Polypeptide Expression
Additional Descriptive Data
Marker for
Subcellular Localization (GO Cellular Component)
CV term Evidence References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools
Reference	See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.
FlyAtlas Anatomy Microarray
FlyAtlas Anatomical Expression Data (FlyAtlas-RNA.adult) (FlyAtlas-RNA.larva)    Styles Linear Logarithmic Heatmap Back-to-back    Scales gene max expression Moderate expression bin max High level expression bin max Very high expression bin max Summary of FlyAtlas Anatomical Expression Data: Expression at high levels in the following post-embryonic organs or tissues: adult ovary. Expression at moderate levels in the following post-embryonic organs or tissues: larval central nervous system, larval salivary gland, larval trachea. [download data (TSV)] Guide to FlyAtlas expression level colors   No expression (0 - 9.999)   Low expression (10 - 99.999)   Moderate expression (100 - 499.999)   High level expression (500 - 999.999)   Very high expression (>999.999) Linear, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary    632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary  (632) Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High  Linear, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary    632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High   Very high  Linear, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary    632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  Very high  log, scaled to maximum expression level Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary    632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Moderate expression Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary  632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High  log, scaled to High level expression Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary    632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High   Very high  log, scaled to Very high expression Tissue   Expression Level Larval Central Nervous System    407.425 Larval Midgut    32.1 Larval Hindgut    53.2 Larval Malpighian Tubules    19.8 Larval Fat Body    30 Larval Salivary Gland    163.7 Larval Trachea    135.2 Larval Carcass    75.2 Adult Head    11.4 Adult Eye    7.525 Adult Brain    12 Adult Thoracic-Abdominal Ganglion    10.9 Adult Crop    21.1 Adult Midgut    29 Adult Hindgut    12.4 Adult Malpighian Tubules    5.7 Adult Fat Body    11 Adult Salivary Gland    4.1 Adult Heart    8.925 Adult VirginFemale Spermatheca    5 Adult InseminatedFemale Spermatheca    9.7 Adult Ovary    632 Adult Testis    12.8 Adult Male Accessory Gland    24.6 Adult Carcass    14 Expression Level Scale  None   Low   Moderate   High   Very high  Heatmap Tissue   Expression Level Larval Central Nervous System     Larval Midgut     Larval Hindgut     Larval Malpighian Tubules     Larval Fat Body     Larval Salivary Gland     Larval Trachea     Larval Carcass     Adult Head     Adult Eye     Adult Brain     Adult Thoracic-Abdominal Ganglion     Adult Crop     Adult Midgut     Adult Hindgut     Adult Malpighian Tubules     Adult Fat Body     Adult Salivary Gland     Adult Heart     Adult VirginFemale Spermatheca     Adult InseminatedFemale Spermatheca     Adult Ovary     Adult Testis     Adult Male Accessory Gland     Adult Carcass     FlyAtlas Organ/Tissue Expression, larval vs. adult Larval Expression Level Tissue Adult Expression Level   NA  Head    no informative data   NA  Eye    7.525   NA  Brain    no informative data   407.425  Central Nervous System    NA   NA  Thoracic-Abdominal Ganglion    no informative data   NA  Crop    no informative data   no informative data  Midgut    no informative data   53.2  Hindgut    no informative data   no informative data  Malpighian Tubules    5.7   no informative data  Fat Body    no informative data   163.7  Salivary Gland    4.1   NA  Heart    8.925   135.2  Trachea    NA   NA  VirginFemale Spermatheca    5   NA  InseminatedFemale Spermatheca    9.7   NA  Ovary    632   NA  Testis    no informative data   NA  Male Accessory Gland    no informative data   75.2  Carcass    no informative data FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)
modENCODE Anatomy RNA-Seq
modENCODE Tissue Expression Data (modENCODE_mRNA-Seq_tissues)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    39 ovary, mated 4-day female    35 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female  (39) ovary, mated 4-day female  (35) testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    39 ovary, mated 4-day female    35 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    39 ovary, mated 4-day female    35 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Extremely high  log, scaled to maximum expression level Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    39 ovary, mated 4-day female    35 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female  (39) ovary, mated 4-day female  (35) testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    39 ovary, mated 4-day female    35 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Tissue   Expression Level imaginal disc, larvae L3 wandering    11 central nervous system, larvae L3    13 central nervous system, pupae P8    1 head, virgin 1-day female    0 head, virgin 4-day female    0 head, virgin 20-day female    0 head, mated 1-day female    0 head, mated 4-day female    0 head, mated 20-day female    0 head, mated 1-day male    1 head, mated 4-day male    0 head, mated 20-day male    1 salivary gland, larvae L3 wandering    0 salivary gland, white prepupae    0 digestive system, larvae L3 wandering    1 digestive system, 1-day adult    1 digestive system, 4-day adult    2 digestive system, 20-day adult    2 fat body, larvae L3 wandering    0 fat body, white prepupae    1 fat body, pupae P8    0 carcass, larvae L3 wandering    7 carcass, 1-day adult    1 carcass, 4-day adult    2 carcass, 20-day adult    1 ovary, virgin 4-day female    39 ovary, mated 4-day female    35 testis, mated 4-day male    2 accessory gland, mated 4-day male    1 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Tissue   Expression Level imaginal disc, larvae L3 wandering     central nervous system, larvae L3     central nervous system, pupae P8     head, virgin 1-day female     head, virgin 4-day female     head, virgin 20-day female     head, mated 1-day female     head, mated 4-day female     head, mated 20-day female     head, mated 1-day male     head, mated 4-day male     head, mated 20-day male     salivary gland, larvae L3 wandering     salivary gland, white prepupae     digestive system, larvae L3 wandering     digestive system, 1-day adult     digestive system, 4-day adult     digestive system, 20-day adult     fat body, larvae L3 wandering     fat body, white prepupae     fat body, pupae P8     carcass, larvae L3 wandering     carcass, 1-day adult     carcass, 4-day adult     carcass, 20-day adult     ovary, virgin 4-day female     ovary, mated 4-day female     testis, mated 4-day male     accessory gland, mated 4-day male
modENCODE Development RNA-Seq
modENCODE Temporal Expression Data (modENCODE_mRNA-Seq_U)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of very high expression to a trough of low expression. Peak expression observed within 00-06 hour embryonic stages. [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    131 embryo 02-04hr    61 embryo 04-06hr    156 embryo 06-08hr    119 embryo 08-10hr    79 embryo 10-12hr    50 embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    44 adult female 05day    66 adult female 30day    73 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (131) embryo 02-04hr  (61) embryo 04-06hr  (156) embryo 06-08hr  (119) embryo 08-10hr  (79) embryo 10-12hr  (50) embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day  (44) adult female 05day  (66) adult female 30day  (73) Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  (131) embryo 02-04hr    61 embryo 04-06hr  (156) embryo 06-08hr  (119) embryo 08-10hr    79 embryo 10-12hr    50 embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    44 adult female 05day    66 adult female 30day    73 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    131 embryo 02-04hr    61 embryo 04-06hr    156 embryo 06-08hr    119 embryo 08-10hr    79 embryo 10-12hr    50 embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    44 adult female 05day    66 adult female 30day    73 Expression Level Scale  Extremely high  log, scaled to maximum expression level Developmental Stage   Expression Level embryo 00-02hr    131 embryo 02-04hr    61 embryo 04-06hr    156 embryo 06-08hr    119 embryo 08-10hr    79 embryo 10-12hr    50 embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    44 adult female 05day    66 adult female 30day    73 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Developmental Stage   Expression Level embryo 00-02hr  (131) embryo 02-04hr  (61) embryo 04-06hr  (156) embryo 06-08hr  (119) embryo 08-10hr  (79) embryo 10-12hr  (50) embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day  (44) adult female 05day  (66) adult female 30day  (73) Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Developmental Stage   Expression Level embryo 00-02hr  131 embryo 02-04hr    61 embryo 04-06hr  (156) embryo 06-08hr  119 embryo 08-10hr    79 embryo 10-12hr    50 embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    44 adult female 05day    66 adult female 30day    73 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Developmental Stage   Expression Level embryo 00-02hr    131 embryo 02-04hr    61 embryo 04-06hr    156 embryo 06-08hr    119 embryo 08-10hr    79 embryo 10-12hr    50 embryo 12-14hr    16 embryo 14-16hr    15 embryo 16-18hr    7 embryo 18-20hr    5 embryo 20-22hr    3 embryo 22-24hr    3 larva L1    18 larva L2    13 larva L3 12hr old    10 larva L3 puffstage 1-2    12 larva L3 puffstage 3-6    16 larva L3 puffstage 7-9    14 white prepupae new    11 white prepupae 12hr    23 white prepupae 24hr    17 pupae 2d postWPP    3 pupae 3d postWPP    2 pupae 4d postWPP    6 adult male 01day    2 adult male 05day    2 adult male 30day    3 adult female 01day    44 adult female 05day    66 adult female 30day    73 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Developmental Stage   Expression Level embryo 00-02hr     embryo 02-04hr     embryo 04-06hr     embryo 06-08hr     embryo 08-10hr     embryo 10-12hr     embryo 12-14hr     embryo 14-16hr     embryo 16-18hr     embryo 18-20hr     embryo 20-22hr     embryo 22-24hr     larva L1     larva L2     larva L3 12hr old     larva L3 puffstage 1-2     larva L3 puffstage 3-6     larva L3 puffstage 7-9     white prepupae new     white prepupae 12hr     white prepupae 24hr     pupae 2d postWPP     pupae 3d postWPP     pupae 4d postWPP     adult male 01day     adult male 05day     adult male 30day     adult female 01day     adult female 05day     adult female 30day     modENCODE Temporal Expression Data (Graveley et al., 2011)
modENCODE Cell Lines RNA-Seq
modENCODE Cell Line Expression Data (modENCODE_mRNA-Seq_cell.A) (modENCODE_mRNA-Seq_cell.B)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    40 embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS    100 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4  (40) embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3  (31) wing disc ML-DmD21  (38) wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1  (29) CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS  (100) ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    40 embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS    100 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    40 embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS    100 ovary OSC    17 ovary OSS    13 Expression Level Scale  Extremely high  log, scaled to maximum expression level Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    40 embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS    100 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Moderate expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4  (40) embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3  31 wing disc ML-DmD21  (38) wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1  29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS  (100) ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    40 embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS    100 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Cell Line   Expression Level Schneider line 2 S2R+    23 Schneider line 2 Sg4    40 embryonic 1182-4H    15 embryonic GM2    16 embryonic Kc167    23 embryonic S1    11 embryonic S3    14 leg disc CME L1    22 wing disc CME-W2    13 wing disc ML-DmD8    21 wing disc ML-DmD9    19 wing disc ML-DmD16-c3    31 wing disc ML-DmD21    38 wing disc ML-DmD32    17 haltere disc ML-DmD17-c3    21 eye-antennal disc ML-DmD11    18 antennal disc ML-DmD20-c5    24 mixed discs ML-DmD4-c1    16 CNS ML-DmBG1-c1    29 CNS ML-DmBG2-c2    15 tumorous blood cells mbn2    6 ovary fGS/OSS    100 ovary OSC    17 ovary OSS    13 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Cell Line   Expression Level Schneider line 2 S2R+     Schneider line 2 Sg4     embryonic 1182-4H     embryonic GM2     embryonic Kc167     embryonic S1     embryonic S3     leg disc CME L1     wing disc CME-W2     wing disc ML-DmD8     wing disc ML-DmD9     wing disc ML-DmD16-c3     wing disc ML-DmD21     wing disc ML-DmD32     haltere disc ML-DmD17-c3     eye-antennal disc ML-DmD11     antennal disc ML-DmD20-c5     mixed discs ML-DmD4-c1     CNS ML-DmBG1-c1     CNS ML-DmBG2-c2     tumorous blood cells mbn2     ovary fGS/OSS     ovary OSC     ovary OSS
modENCODE Treatments RNA-Seq
modENCODE Treatment Expression Data (modENCODE_mRNA-Seq_treatments)    Styles Linear Logarithmic Heatmap    Scales gene max expression Moderate expression bin max High expression bin max Extremely high expression bin max [download data (TSV)] Guide to modENCODE expression level colors   No/Extremely low expression (0 - 0)   Very low expression (1 - 3)   Low expression (4 - 10)   Moderate expression (11 - 25)   Moderately high expression (26 - 50)   High expression (51 - 100)   Very high expression (101 - 1000)   Extremely high expression (>1000) Linear, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult  26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3  (42) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  Linear, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  Linear, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Extremely high  log, scaled to maximum expression level Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High  log, scaled to Moderate expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult  26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3  (42) Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high  log, scaled to High expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high  log, scaled to Extremely high expression Treatment   Expression Level extended cold, 4-day adult    7 cold shock, 4-day adult    4 heat shock, 4-day adult    15 Cadmium 50 mM 6 hrs, larvae L3    5 Cadmium 50 mM 12 hrs, larvae L3    2 Cadmium 50 mM 48 hrs, 4-day adult    10 Cadmium 100 mM 48 hrs, 4-day adult    26 Copper 0.5 mM 12 hrs, larvae L3    5 Copper 15 mM 48 hrs, 4-day adult    10 Zinc 5 mM 12 hrs, larvae L3    2 Zinc 4.5 mM 48 hrs, 4-day adult    3 Ethanol 2.5% 3 hrs, larvae L3    42 Ethanol 5% 3 hrs, larvae L3    3 Ethanol 10% 3 hrs, larvae L3    4 Caffeine 1.5 mg/ml 4 hrs, larvae L3    2 Caffeine 2.5 mg/ml 48 hrs, 4-day adult    20 Caffeine 25 mg/ml 48 hrs, 4-day adult    7 Paraquat 5 mM 48 hrs, 4-day adult    20 Paraquat 10 mM 48 hrs, 4-day adult    21 Rotenone 2 μg 12 hrs, larvae L3    4 Rotenone 8 μg 12 hrs, larvae L3    5 Expression Level Scale  Very low   Low   Moderate   Moderately high   High   Very high   Extremely high  Heatmap Treatment   Expression Level extended cold, 4-day adult     cold shock, 4-day adult     heat shock, 4-day adult     Cadmium 50 mM 6 hrs, larvae L3     Cadmium 50 mM 12 hrs, larvae L3     Cadmium 50 mM 48 hrs, 4-day adult     Cadmium 100 mM 48 hrs, 4-day adult     Copper 0.5 mM 12 hrs, larvae L3     Copper 15 mM 48 hrs, 4-day adult     Zinc 5 mM 12 hrs, larvae L3     Zinc 4.5 mM 48 hrs, 4-day adult     Ethanol 2.5% 3 hrs, larvae L3     Ethanol 5% 3 hrs, larvae L3     Ethanol 10% 3 hrs, larvae L3     Caffeine 1.5 mg/ml 4 hrs, larvae L3     Caffeine 2.5 mg/ml 48 hrs, 4-day adult     Caffeine 25 mg/ml 48 hrs, 4-day adult     Paraquat 5 mM 48 hrs, 4-day adult     Paraquat 10 mM 48 hrs, 4-day adult     Rotenone 2 μg 12 hrs, larvae L3     Rotenone 8 μg 12 hrs, larvae L3
Expression Clusters
	A cluster of genes with similar mRNA expression dynamics across development. (The modENCODE Consortium, 2010, Graveley et al., 2011) mE2_34_mRNA_expression_cluster_13 mE1_20_mRNA_expression_cluster_04
External Data & Images
Linkouts	BDGP expression data • LD07144 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024332 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4206-RA FlyExpress - Embryonic expression images (BDGP data) • Show all FlyExpress processed images for Mcm3 Stages(s) 1-3 Stages(s) 4-6 Stages(s) 7-8 Stages(s) 11-12 Stages(s) 13-16
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele lethal Mcm3GD4249 Other Phenotypes Allele neuroanatomy defective | larval stage Mcm3GD4249 radiation sensitive, with Scer\GAL4ap-md544 Mcm3NUG.cUa Phenotype manifest in Allele embryonic/larval neuroblast | larval stage Mcm3GD4249 ganglion mother cell | larval stage Mcm3GD4249
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Mcm3 Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 5 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Mcm3 Class Mutagen Stocks Known lesion Mcm3GD4249 in vitro construct - RNAi in vitro construct - regulatory fusion 2 Yes Mcm3HMS01164 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mcm3KK102079 in vitro construct - RNAi in vitro construct - regulatory fusion 1 Yes Mcm3NIG.4206R in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes Mcm3NUG.cUa in vitro construct - RNAi in vitro construct - regulatory fusion 0 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD4249} NA P{KK102079} NA P{NIG.4206R} NA P{TRiP.HMS01164} NA characterization construct P{NIG.Mcm3.U} NA
Insertions
	Type of insertions Name Expression data
Gene Ontology: Function, Process & Cellular Component ( 10 unique terms )
Terms Based on Experimental Evidence ( 3 terms )
Molecular Function
CV term Evidence References
contributes_to 3'-5' DNA helicase activity inferred from direct assay (Moyer et al., 2006)
Biological Process
CV term Evidence References
mitotic G2 DNA damage checkpoint inferred from mutant phenotype (Kondo and Perrimon, 2011) neurogenesis inferred from mutant phenotype (Neumüller et al., 2011)
Cellular Component ( 0 terms)
Terms Based on Predictions or Assertions ( 7 terms )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008046, InterPro:IPR018525 (FlyBase Curators et al., 2004-) chromatin binding inferred from sequence or structural similarity with Saccharomyces MCM3 (FlyBase, 1992-) non-traceable author statement (Feger, 1999.2.1) DNA binding inferred from electronic annotation with InterPro:IPR001208, InterPro:IPR008046, InterPro:IPR018525 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
DNA replication initiation inferred from electronic annotation with InterPro:IPR008046 (FlyBase Curators et al., 2004-) pre-replicative complex assembly inferred from sequence or structural similarity with Saccharomyces MCM3 (FlyBase, 1992-) non-traceable author statement (Feger, 1999.2.1)
Cellular Component
CV term Evidence References
MCM complex inferred from electronic annotation with InterPro:IPR008046 (FlyBase Curators et al., 2004-) pre-replicative complex inferred from sequence or structural similarity with Saccharomyces MCM3 (FlyBase, 1992-) non-traceable author statement (Feger, 1999.2.1)
Sequence Ontology: Class of Gene
	nuclear_gene   protein_coding_gene
Interactions & Pathways
Summary of Physical Interactions
protein-protein
Interacting group Assay References
Mcm3 - 14-3-3ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - AP-1-2β anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - ATPsyn-γ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Ahcy13 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Art8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Bap55 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CDC45L anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG10306 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG14667 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG15784 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG31358 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG31694 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG3430 experimental knowledge based, anti tag coimmunoprecipitation, peptide massfingerprinting (Guruharsha et al., 2011, Mintseris et al., 2009.11.23) Mcm3 - CG6084 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG6937 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG8036 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - CG8545 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Cctγ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Droj2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Gale anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - GstE8 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hcf anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hsc70-1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hsp22 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hsp23 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hsp27 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hsp68 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Hsp70Aa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Kap-α3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - LKR anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Mcm5 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Mcm3 - Mcm6 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Mcm7 anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based (Mintseris et al., 2009.11.23, Guruharsha et al., 2011) Mcm3 - Nurf-38 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Prat anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Rab2 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - RpS10a anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Scox anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Tcp-1ζ anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - Trap1 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - cathD anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - dpa anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - grsm anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - l(1)G0320 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - msn anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - mts anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - nop5 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - ref(2)P anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - smt3 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23) Mcm3 - ttm50 anti tag coimmunoprecipitation, peptide massfingerprinting (Mintseris et al., 2009.11.23)
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Mcm3 allele Gene References
External Data
Linkouts	BioGRID - A database of protein and genetic interactions • 57959 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024332 DroID - A comprehensive database of gene and protein interactions. • FBgn0024332 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31449
Orthologs
OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41
OrthoDB Ortholog Groups
	OrthoDB orthology group searches (Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288) Drosophila inclusive ortholog search EOG62RDSS Dipteran inclusive ortholog search EOG63BMWZ Insect inclusive ortholog search EOG67M0SW Arthropod inclusive ortholog search EOG6WDBST Metazoa inclusive ortholog search EOG6T7MW4
Orthologs in Drosophila Species (EOG62RDSS)
Organism Common Name Gene AAA Syntenic Ortholog Multiple Dmel Genes in this Orthologous Group Drosophila melanogaster fruit fly  Dmel\Mcm3     Drosophila simulans   Dsim\Mcm3 Y   Drosophila sechellia   Dsec\GM12401 Y   Drosophila erecta   Dere\Mcm3 Y   Drosophila yakuba   Dyak\Mcm3 Y   Drosophila ananassae   Dana\GF21337 Y   Drosophila pseudoobscura pseudoobscura   Dpse\GA18030 Y   Drosophila persimilis   Dper\GL14679 Y   Drosophila willistoni   Dwil\GK18502 Y   Drosophila virilis   Dvir\GJ14777 Y   Drosophila mojavensis   Dmoj\GI11075 Y   Drosophila grimshawi   Dgri\GH24613 Y
Orthologs in non-Drosophila Dipterans (EOG63BMWZ)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Aedes aegypti Yellow fever mosquito  Aaeg\AAEL011811   Anopheles gambiae Malaria mosquito  Agam\AGAP001135   Culex quinquefasciatus Southern house mosquito  Cqui\CPIJ012309
Orthologs in non-Dipteran Insects (EOG67M0SW)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Apis mellifera Western honey bee  Amel\Mcm3   Nasonia vitripennis Parasitic wasp  Nvit\Nasvi2EG010679   Acromyrmex echinatior Panamanian leafcutter ant  Aech\AECH11277   Atta cephalotes Leafcutter ant  Acep\ACEP14256   Camponotus floridanus Florida carpenter ant  Cflo\CFLO19622   Harpegnathos saltator Jerdons jumping ant  Hsal\HSAL15707   Linepithema humile Argentine ant  Lhum\LH16331   Pogonomyrmex barbatus Red harvester ant  Pbar\PB17566   Acyrthosiphon pisum Pea aphid  Apim\ACYPI005644   Bombyx mori Silkmoth  Bmor\BGIBMGA013590   Pediculus humanus Human body louse  Phum\PHUM496000   Tribolium castaneum Red flour beetle  Tcas\TC002088
Orthologs in non-Insect Arthropods (EOG6WDBST)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Daphnia pulex Water flea  Dpux\Sccpdh   Ixodes scapularis Deer tick  Isca\ISCW023354
Orthologs in non-Arthropod Metazoa (EOG6T7MW4)
Organism Common Name Gene Multiple Dmel Genes in this Orthologous Group Caenorhabditis elegans Nematode  Cele\mcm-3   Strongylocentrotus purpuratus Purple sea urchin  Spur\SPU_012983gn   Danio rerio Zebrafish  Drer\mcm3   Danio rerio Zebrafish  Drer\mcm3l   Danio rerio Zebrafish  Drer\mcm3   Xenopus tropicalis Western clawed frog  Xtro\mcm3   Xenopus tropicalis Western clawed frog  Xtro\ENSXETG00000021701   Gallus gallus Domestic chicken  Ggal\MCM3   Mus musculus House mouse  Mmus\Mcm3   Rattus norvegicus Norway rat  Rnor\Mcm3   Homo sapiens Human  Hsap\MCM3
Human Orthologs (1)
Gene OMIM HGNC Hsap\MCM3
AAA Orthologs (11) based on analysis using Dmel annotation version 4.3
Organism Gene Drosophila simulans Dsim\Mcm3 Drosophila sechellia Dsec\GM12401 Drosophila erecta Dere\Mcm3 Drosophila yakuba Dyak\Mcm3 Drosophila ananassae Dana\GF21337 Drosophila pseudoobscura pseudoobscura Dpse\GA18030 Drosophila persimilis Dper\GL14679 Drosophila willistoni Dwil\GK18502 Drosophila virilis Dvir\GJ14777 Drosophila mojavensis Dmoj\GI11075 Drosophila grimshawi Dgri\GH24613
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Bloomington	34686 y1 sc* v1; P{TRiP.HMS01164}attP2
VDRC	v108214 P{KK102079}VIE-260B v51217 w1118 P{GD4249}v51217 v51216 w1118; P{GD4249}v51216/TM3
Genomic Clones ( 1 )
	BACR01M22 Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
cDNA Clones ( 33 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	LP01948
Other clones	DPiM_LP01948
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	LD12563 LD15592 LD26235 LD30950 LD35145 LD36714 SD01611 SD09457 SD13214
Other clones	523294 Dmel_neb_014777_0645_1487 EN03761 FGC017C08 IP02416 IP17658 IP17958 LD07144 RP004156178 RP004211648 RP004214470 RP004232789 RP004239880 RP004247610 RP004252147 RP004356720 RP004382917 RP004425401 RP004434893 RP004507938 RP004539092 RP004546525
RNAi & Array Information
Linkouts	DRSC - Results from RNAi screens. • FBgn0024332 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31449
Antibody Information
Other Information
Discoverer
Etymology
Identification
Relationship to Other Genes
Source for database identity of	Source for identity of: Mcm3 CG4206 (Zhong, 1999.11)
Source for database merge of	Source for merge of: Mcm3 Mcp-PCR2 (Ohno et al., 1998)
Additional comments
Other Comments
	The Sld5, Psf1, Psf2, Psf3, CDC45L, Mcm2, Mcm3, Mcm5, Mcm6, Mcm7 and dpa proteins form the Cdc45/Mcm2-7/GINS (CMG) complex, a helicase required for the S phase transition in Kc cells. (Moyer et al., 2006)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_078493 RefSeq (Proteins) • NP_511048 DDBJ / EMBL / GenBank • AA263937 AAC47651 AAD32859 AAF46023 AB010107 AF124745 AW941632 BAA34731 U83492 Entrez Gene - A searchable database of RefSeq genes. • 31449 UniProtKB/Swiss-Prot • Q9XYU1 UniProtKB/TrEMBL • Q7JNE2
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Mini-chromosome maintenance, conserved site (IPR018525) Mini-chromosome maintenance, DNA-dependent ATPase (IPR001208) AAA+ ATPase domain (IPR003593) DNA replication licensing factor Mcm3 (IPR008046) Nucleic acid-binding, OB-fold (IPR012340) P-loop containing nucleoside triphosphate hydrolase (IPR027417) BDGP expression data - Patterns of gene expression in Drosophila embryogenesis • LD07144
Linkouts
	BioGRID - A database of protein and genetic interactions • 57959 DPiM - DPiM, Drosophila Protein interaction Map • FBgn0024332 DroID - A comprehensive database of gene and protein interactions. • FBgn0024332 DRSC - Results from RNAi screens. • FBgn0024332 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024332 FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array • CG4206-RA FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024332 GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents • 31449 InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets. • 31449 modMine - Data generated by the modENCODE project. • FBgn0024332
Synonyms & Secondary IDs ( 12 )
Reported As
Symbol Synonym	CG4206 (Keleman et al., 2009.8.5, Ni et al., 2010.12.1) dMCM3 (Duronio et al., 1998) DmeMCM3 (Blanton et al., 2005) DmMcm3 (Loebel et al., 2000) DmMCM3 (Ohno, 1998.1.5, Feger, 1999, Ohno et al., 1998) MCM3 (Claycomb et al., 2002, Stevaux et al., 2002, Crevel et al., 2001, Frolov et al., 2001, Crevel et al., 2007, Hediger and Gasser, 2006, Frolov et al., 2005, Li et al., 2010, Crevel et al., 2011, Hong et al., 2007, Ilves et al., 2010, Gallone et al., 2011) Mcm3 (Akashi et al., 2006, Moyer et al., 2006, Ko et al., 2006, De Lucia et al., 2005, Foglietti et al., 2006, Cayirlioglu et al., 2003, Somma et al., 2008, Costa et al., 2011, Maqbool et al., 2010, Spresser et al., 2008, Longworth et al., 2012, Kondo and Perrimon, 2011, Neumüller et al., 2011, Loop et al., 2004, Japanese National Institute of Genetics, 2012.5.21) McM3 (De Lucia et al., 2005) Mcp-PCR2   PCR2 (Su et al., 1997)
Name Synonym	Minichromosome maintenance 3 (Duronio et al., 1998, Somma et al., 2008) Minichromosome maintenance-PCR2
Secondary FlyBase IDs
	FBgn0020632
References ( 65 )
Generate a list of	All references relating to this gene ( 65 )
List References by type	Research paper  ( 36 ) Supplementary material  ( 5 ) Review  ( 3 ) Personal communication to FlyBase  ( 6 ) FlyBase analysis  ( 2 ) DNA/RNA sequence record  ( 7 ) Stock list  ( 1 ) Curated genome annotation  ( 3 ) Website  ( 1 )
Recent research papers ( 8 )
	Ilves et al., 2012, Proc. Natl. Acad. Sci. U.S.A. 109(33): 13163--13170 Checkpoint kinase 2 (Chk2) inhibits the activity of the Cdc45/MCM2-7/GINS (CMG) replicative helicase complex. [FBrf0219136] Krauchunas et al., 2012, Dev. Biol. 370(1): 125--134 Protein phosphorylation changes reveal new candidates in the regulation of egg activation and early embryogenesis in D. melanogaster. [FBrf0219342] Longworth et al., 2012, PLoS Genet. 8(4): e1002618 A Shared Role for RBF1 and dCAP-D3 in the Regulation of Transcription with Consequences for Innate Immunity. [FBrf0218058] Costa et al., 2011, Nat. Struct. Mol. Biol. 18(4): 471--477 The structural basis for MCM2-7 helicase activation by GINS and Cdc45. [FBrf0214314] Crevel et al., 2011, PLoS ONE 6(11): e27101 Decreased MCM2-6 in Drosophila S2 Cells Does Not Generate Significant DNA Damage or Cause a Marked Increase in Sensitivity to Replication Interference. [FBrf0216797] Gallone et al., 2011, BMC Bioinformatics 12: 289 Bio::Homology::InterologWalk - A Perl module to build putative protein-protein interaction networks through interolog mapping. [FBrf0214774] Kondo and Perrimon, 2011, Sci. Signal. 4(154): rs1 A Genome-Wide RNAi Screen Identifies Core Components of the G2-M DNA Damage Checkpoint. [FBrf0212700] Neumüller et al., 2011, Cell Stem Cell 8(5): 580--593 Genome-Wide Analysis of Self-Renewal in Drosophila Neural Stem Cells by Transgenic RNAi. [FBrf0213621] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2011 Show reviews ...