FlyBase Gene Report: Dmel\E2f2

General Information

Symbol

Dmel\E2f2

Species

D. melanogaster

Name

E2F transcription factor 2

Annotation Symbol

CG1071

Feature Type

protein_coding_gene

FlyBase ID

FBgn0024371

Gene Model Status

Current

Stock Availability

14 publicly available

Gene Snapshot

E2F transcription factor 2 (E2f2) encodes a transcription factor that belongs to the E2F gene family. It is a subunit of the DREAM complex, where it forms a heterodimer with the product of Dp to bind to DNA and repress transcription. Among their trancription targets are cell cycle genes and differentiation related genes. [Date last reviewed: 2018-09-20]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

dE2F2, E2f

Key Links

Genomic Location

Cytogenetic map

39A5-39A6

Sequence location

2L:21,154,293..21,156,749 [-]

Recombination map

2-54

RefSeq locus

NT_033779 REGION:21154293..21156749

Sequence

Get Decorated FASTA

Genomic Maps

GBrowse JBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

[Detailed GO annotations]

Gene Group (FlyBase)

E2F TRANSCRIPTION FACTORS

Protein Family (UniProt)

Belongs to the E2F/DP family. (O77051)

Protein Signatures (InterPro)

Molecular Function (GO)
[Detailed GO annotations]

Experimental Evidence

contributes_to DNA-binding transcription repressor activity, RNA polymerase II-specific; protein binding; RNA polymerase II regulatory region DNA binding; transcription factor binding

Predictions / Assertions

DNA binding; DNA-binding transcription factor activity; DNA-binding transcription factor activity, RNA polymerase II-specific; sequence-specific DNA binding; transcription factor binding

Summaries

Gene Group (FlyBase)

E2F TRANSCRIPTION FACTORS -: The E2F/DP family of transcription factors are sequence-specific DNA-binding proteins that regulate transcription. The functional E2F are transcriptional heterodimers composed of two subunits, E2F and DP. (Adapted from FBrf0128583).

Protein Function (UniProtKB)

Transcriptional repressor that binds to E2f sites and represses E2f-regulated target genes. Binding to E2f sites requires transcription factor Dp. Acts synergistically with Rbf2 to antagonize E2f1-mediated transcriptional activation. Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. The DREAM complex is required for recruiting E2f2 at differentiation-specific promoters and for stabilizing E2f2-Rbf complexes during S phase. During development, the complex represses transcription of developmentally controlled E2f target genes. During oogenesis, plays a role in restricting DNA synthesis to sites of chorion gene amplification in late stage ovarian follicle cells. Plays an inhibitory role in ionizing radiation (IR)-induced p53-independent apoptosis. May be involved in cell cycle exit by temporarily limiting CycE-dependent activation of E2f-regulated transcription.

(UniProt, O77051)

Summary (Interactive Fly)

negatively regulates genes involved in the S (DNA synthesis) phase of the cell cycle - Retinoblastoma family 2 is required for the tissue-specific repression of dE2F2 target genes

Interactive Fly

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the GBrowse view of Dmel\E2f2 or the JBrowse view of Dmel\E2f2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

E2f2-RA

FBtr0081501

NM_058007

1485

370

E2f2-RB

FBtr0346451

NM_001299186

2002

370

Additional Transcript Data and Comments

Reported size (kB)

1.4 (northern blot)

(Sawado et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

E2f2-PA

FBpp0081029

41.4

370

4.81

NP_477355

AAF53965

E2f2-PB

FBpp0312101

41.4

370

4.81

NP_001286115

AHN54629

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

370 aa isoforms: E2f2-PA, E2f2-PB

Additional Polypeptide Data and Comments

Reported size (kDa)

370 (aa); 41.4 (kD predicted)

(Sawado et al., 1998)

Comments

External Data

Subunit Structure (UniProtKB)

Forms a heterodimer with Dp. Interacts with Rbf/Rbf1 and Rbf2. Component of the DREAM complex, which is at least composed of Myb, Caf1, mip40, mip120, mip130, E2f2, Dp, Rbf, Rbf2, lin-52, Rpd3 and l(3)mbt.

(UniProt, O77051)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\E2f2 using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

E2f2_1

Linkouts

Gene Ontology (26 terms)

Molecular Function (8 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

contributes_to DNA-binding transcription repressor activity, RNA polymerase II-specific

inferred from physical interaction with FLYBASE:Rbf2; FB:FBgn0038390

(Stevaux et al., 2002)

protein binding

inferred from physical interaction with UniProtKB:Q24472

(assigned by UniProt )

(Stevaux et al., 2002, Frolov et al., 2001)

inferred from physical interaction with UniProtKB:Q9NIS1

(assigned by UniProt )

(Stevaux et al., 2002)

RNA polymerase II regulatory region DNA binding

inferred from direct assay

(Lee et al., 2012)

inferred from mutant phenotype

(Kim et al., 2018)

transcription factor binding

inferred from physical interaction with FLYBASE:Rbf2; FB:FBgn0038390

(Stevaux et al., 2002)

Terms Based on Predictions or Assertions (5 terms)

CV Term

Evidence

References

DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

DNA-binding transcription factor activity

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

DNA-binding transcription factor activity, RNA polymerase II-specific

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

sequence-specific DNA binding

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

transcription factor binding

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

Biological Process (13 terms)

Terms Based on Experimental Evidence (7 terms)

CV Term

Evidence

References

DNA endoreduplication

inferred from mutant phenotype

(Weng et al., 2003)

inferred from genetic interaction with FLYBASE:CycE; FB:FBgn0010382

(Weng et al., 2003)

negative regulation of apoptotic process

inferred from direct assay

(assigned by UniProt )

(Wichmann et al., 2010)

negative regulation of DNA replication

inferred from mutant phenotype

(Cayirlioglu et al., 2003, Cayirlioglu et al., 2001)

negative regulation of G1/S transition of mitotic cell cycle

inferred from direct assay

(Stevaux et al., 2002)

negative regulation of transcription by RNA polymerase II

inferred from mutant phenotype

(Korenjak et al., 2004, Lewis et al., 2004)

negative regulation of transcription, DNA-templated

inferred from direct assay

(assigned by UniProt )

(Taylor-Harding et al., 2004, Sawado et al., 1998)

positive regulation of transcription regulatory region DNA binding

inferred from mutant phenotype

(Kim et al., 2018)

Terms Based on Predictions or Assertions (8 terms)

CV Term

Evidence

References

endomitotic cell cycle

traceable author statement

(Lee and Orr-Weaver, 2003)

G1/S transition of mitotic cell cycle

traceable author statement

(Lee and Orr-Weaver, 2003)

imaginal disc development

traceable author statement

(Lee and Orr-Weaver, 2003)

negative regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

negative regulation of transcription, DNA-templated

traceable author statement

(Lee and Orr-Weaver, 2003)

oogenesis

traceable author statement

(Lee and Orr-Weaver, 2003)

positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

regulation of cell cycle

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

Cellular Component (5 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

chromatin

inferred from direct assay

(Korenjak et al., 2004)

Myb complex

inferred from direct assay

(Korenjak et al., 2004, Lewis et al., 2004)

nucleus

inferred from high throughput direct assay

(Sarov et al., 2016)

Rb-E2F complex

inferred from direct assay

(Stevaux et al., 2002)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

RNA polymerase II transcription factor complex

inferred from biological aspect of ancestor with PANTHER:PTN000249624

(assigned by GO_Central )

(Gaudet et al., 2011)

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 3 -- 4

organism

Comment: maternally supplied

(Cayirlioglu et al., 2001)

embryonic stage 5 -- 10

cell

Comment: mitotically active cells

(Cayirlioglu et al., 2001)

embryonic stage 12 -- 14

neuroblast

Comment: mitotically active cells

(Cayirlioglu et al., 2001)

embryonic/larval midgut primordium

Comment: endoreplicating cells

(Cayirlioglu et al., 2001)

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut anlage

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral ectoderm

(Fisher et al., 2012)

ventral nerve cord primordium

Comment: reported as ventral nerve cord anlage

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

inclusive hindgut primordium

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

hindgut proper primordium

(Fisher et al., 2012)

Malpighian tubule primordium

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

somatic muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

visceral muscle primordium

Comment: reported as muscle system primordium

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Sawado et al., 1998)

adult stage

ovary

(Cayirlioglu et al., 2001)

Additional Descriptive Data

E2f2 transcript is detected in all developmental stages, with highest levels in 4-8 hr embryos, and low levels in unfertilized eggs, larvae, pupae, and adults.

(Sawado et al., 1998)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

chromatin

inferred from direct assay

(Korenjak et al., 2004)

Myb complex

inferred from direct assay

(Korenjak et al., 2004, Lewis et al., 2004)

nucleus

inferred from high throughput direct assay

(Sarov et al., 2016)

Rb-E2F complex

inferred from direct assay

(Stevaux et al., 2002)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\E2f2 in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\E2f2

View exonic expression by tissue for Dmel\E2f2

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024371

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0024371

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1071-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1071

Flygut - An atlas of the Drosophila adult midgut

FBgn0024371

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\E2f2

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 12 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 17 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of E2f2

Transgenic constructs containing regulatory region of E2f2

Deletions and Duplications ( 3 )

Disrupted in

Df(2L)Exel6047

(Ryder, 2004.4.26)

Df(2L)G5.1

(Frolov et al., 2001)

Df(2L)Q3.1

(Frolov et al., 2001)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

partially lethal

E2f2^76Q1

partially lethal - majority live (with Df(2L)pr-A14)

E2f2^76Q1

partially lethal - majority live (with Df(2L)TW1)

E2f2^76Q1

viable

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

E2f2^GD4194

viable, with E2f2^1-188

E2f2³²⁹

viable, with E2f2³²⁹

E2f2^1-188

viable, with Scer\GAL4^pnr-MD237

E2f2^GD4194

viable, with Scer\GAL4^tin.CΔ4

E2f2^GD4194

Sterility

Allele

female semi-sterile, with E2f2³²⁹/Df(2L)DS8

E2f2^1-188

female semi-sterile, with E2f2^k16402a/Df(2L)DS8

E2f2^1-188

female semi-sterile (with Df(2L)DS8), with E2f2^1-188

female semi-sterile | recessive

E2f2³²⁹

fertile

E2f2^EY02995

male fertile

E2f2³²⁹

semi-fertile

E2f2^76Q1

Other Phenotypes

Allele

visible, with Scer\GAL4^GMR.PF

E2f2^UAS.cFa

wild-type

E2f2^+t6.4

Phenotype manifest in

Allele

chorion

chorion, with Df(2L)DS8, E2f2^1-188

chorion, with Df(2L)DS8, E2f2³²⁹

E2f2^1-188

chorion, with Df(2L)DS8, E2f2^k16402a

E2f2^1-188

chorion, with E2f2³²⁹/Df(2L)DS8

E2f2^1-188

chorion, with E2f2^k16402a/Df(2L)DS8

E2f2^1-188

chorion (with Df(2L)DS8), with E2f2^1-188

eye, with Scer\GAL4^GMR.PF

E2f2^UAS.cFa

follicle cell, with E2f2^1-188

E2f2³²⁹

follicle cell, with E2f2³²⁹

E2f2^1-188

follicle cell & nucleus

E2f2³²⁹

follicle cell & nucleus, with Df(2L)DS8, E2f2^1-188

follicle cell & nucleus, with Df(2L)DS8, E2f2³²⁹

E2f2^1-188

follicle cell & nucleus, with Df(2L)DS8, E2f2^k16402a

E2f2^1-188

follicle cell & nucleus, with E2f2³²⁹/Df(2L)DS8

E2f2^1-188

follicle cell & nucleus, with E2f2^k16402a/Df(2L)DS8

E2f2^1-188

follicle cell & nucleus (with Df(2L)DS8), with E2f2^1-188

ommatidium, with Scer\GAL4^GMR.PF

E2f2^UAS.cFa

salivary gland & nucleus (with E2f2^76Q1)

E2f2^p111-5

salivary gland & nucleus (with E2f2^p111-5)

E2f2^76Q1

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v7.1)

Homo sapiens (Human) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\E2F5

9 of 15

Yes

Hsap\E2F4

7 of 15

Yes

5 of 15

4 of 15

3 of 15

3 of 15

Model Organism Orthologs (via DIOPT v7.1)

Mus musculus (laboratory mouse) (6)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\E2f4

6 of 15

Yes

Mmus\E2f3

5 of 15

Mmus\E2f5

5 of 15

Yes

Mmus\E2f1

4 of 15

Mmus\E2f2

3 of 15

Mmus\E2f6

3 of 15

Rattus norvegicus (Norway rat) (6)

Rnor\E2f4

6 of 13

Yes

Rnor\E2f5

6 of 13

Yes

3 of 13

3 of 13

3 of 13

2 of 13

Xenopus tropicalis (Western clawed frog) (4)

Xtro\e2f5

7 of 12

Yes

Xtro\e2f4

5 of 12

Yes

Xtro\e2f1

1 of 12

Xtro\e2f3

1 of 12

Danio rerio (Zebrafish) (6)

Drer\e2f5

8 of 15

Yes

Drer\e2f4

7 of 15

Yes

Drer\e2f3

4 of 15

Drer\si:ch211-160f23.5

3 of 15

Drer\si:ch211-195d17.2

3 of 15

Drer\e2f1

1 of 15

Caenorhabditis elegans (Nematode, roundworm) (2)

Cele\efl-1

8 of 15

Yes

Cele\efl-2

3 of 15

Yes

Arabidopsis thaliana (thale-cress) (3)

Atha\E2F1

4 of 9

Yes

Atha\E2F3

4 of 9

Yes

Atha\ATE2F2

3 of 9

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (0)

No records found.

Orthologs in Drosophila Species (via OrthoDB v9.1) ( EOG09190ALF )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Dmel\E2f2

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA10512

Drosophila persimilis

Dper\GL25864

Drosophila willistoni

Dwil\GK21054

Drosophila virilis

Dvir\GJ16286

Drosophila mojavensis

Dmoj\GI14521

Drosophila grimshawi

Dgri\GH13220

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091506GC )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17026898

Glossina morsitans

Tsetse fly

Gmor\GMOY002409

Glossina morsitans

Tsetse fly

Gmor\GMOY000201

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07074

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( None identified )

No non-Dipteran orthologies identified

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( None identified )

No non-Insect Arthropod orthologies identified

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( None identified )

No non-Arthropod Metazoa orthologies identified

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v7.1)

Drosophila melanogaster (Fruit fly) (1)

Gene Symbol

Score

Source

Alignment

5 of 10

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Allele

Disease

Interaction

References

Disease Associations of Human Orthologs (via DIOPT v7.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

E2F5; E2F transcription factor 5

9 of 15

600967

E2F4; E2F transcription factor 4

7 of 15

600659

E2F3; E2F transcription factor 3

5 of 15

600427

E2F1; E2F transcription factor 1

4 of 15

189971

E2F2; E2F transcription factor 2

3 of 15

600426

E2F6; E2F transcription factor 6

3 of 15

602944

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

E2f2 - Ada2b

anti tag coimmunoprecipitation, peptide massfingerprinting

(Weake et al., 2011)

E2f2 - Cp190

anti bait coimmunoprecipitation, western blot

(Korenjak et al., 2014)

E2f2 - Dp

anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based

(Rhee et al., 2014)

E2f2 - Irbp18

two hybrid array

(Shokri et al., 2019)

E2f2 - Myb

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation

(Wen et al., 2008, Beall et al., 2007, Lewis et al., 2004)

E2f2 - Rbf

anti bait coimmunoprecipitation, western blot, anti tag coimmunoprecipitation, peptide massfingerprinting, experimental knowledge based, anti tag western blot, pull down, autoradiography

(Rhee et al., 2014, Nicolay et al., 2011, Acharya et al., 2010, Korenjak et al., 2004)

E2f2 - Rbf2

anti tag coimmunoprecipitation, anti tag western blot, anti bait coimmunoprecipitation, western blot

(Korenjak et al., 2004)

E2f2 - Su(H)

two hybrid array

(Shokri et al., 2019)

E2f2 - Ubx

bimolecular fluorescence complementation, fluorescence microscopy

(Bischof et al., 2018)

E2f2 - lin-52

molecular sieving, western blot, anti tag coimmunoprecipitation

(Lewis et al., 2012)

E2f2 - mip120

anti bait coimmunoprecipitation, western blot

(Lewis et al., 2004)

E2f2 - mip130

anti bait coimmunoprecipitation, western blot

(Lewis et al., 2004)

E2f2 - mip40

anti bait coimmunoprecipitation, western blot, affinity chromatography technology, Identification by mass spectrometry

(Beall et al., 2007)

E2f2 - slmb

pull down, autoradiography

(Heriche et al., 2003)

Summary of Genetic Interactions

esyN Network Diagram

esyN Network Key:

Suppression

Enhancement

View in Interactions Browser

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

E2f2|E2f1

suppressible

CycE

(Weng et al., 2003)

E2f2

enhanceable

E2f1

(Weng et al., 2003, Frolov et al., 2001)

E2f2

suppressible

E2f1

(Cayirlioglu et al., 2003, Frolov et al., 2001)

E2f2

suppressible

Mcm2

(Cayirlioglu et al., 2003)

E2f2

suppressible

Orc2

(Cayirlioglu et al., 2003)

E2f2

suppressible

Orc5

(Cayirlioglu et al., 2003)

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

E2f1

suppressible

E2f2

(Ambrus et al., 2007, Frolov et al., 2001)

gcm

suppressible

E2f2

(Popkova et al., 2012)

External Data

Subunit Structure (UniProtKB)

(UniProt, O77051 )

Linkouts

BioGRID - A database of protein and genetic interactions.

61336

DroID - A comprehensive database of gene and protein interactions.

FBgn0024371

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35381

MIST (genetic) - An integrated Molecular Interaction Database

E2f2

MIST (protein-protein) - An integrated Molecular Interaction Database

E2f2

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

TGF-beta signaling pathway

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

2-54

Cytogenetic map

39A5-39A6

Sequence location

2L:21,154,293..21,156,749 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

39A5-39A6

Limits computationally determined from genome sequence between P{PZ}l(2)05287⁰⁵²⁸⁷ and P{lacW}l(2)k07215^k07215&P{lacW}snRNA:U4:39B^k09410

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

39A-39A

(Huen et al., 1998)

Experimentally Determined Recombination Data

Location

2-54

(FlyBase, 2016)

2-54.5

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (14)

Bloomington

27995

y¹ v¹; P{TRiP.JF02828}attP2

83675

y¹ w^*; PBac{E2f2-GFP.FPTB}VK00033

17314

y¹ w^67c23; P{EPgy2}E2f2^EY02995

78973

y¹ w^*; TI{CRIMIC.TG4.0}E2f2^{CR00656-TG4.0}/SM6a

78707

y¹ sc^* v¹ sev²¹; P{TOE.GS02103}attP40

5329

y¹ w^67c23; P{lacW}Mpp6^k16403 P{lacW}k16403b/CyO

FlyORF

F003334

M{UAS-E2f2.ORF-CC}ZH-21F

F000069

M{UAS-E2f2.ORF.3xHA.GW}ZH-86Fb

VDRC

v100990

P{KK106480}VIE-260B

v45743

w¹¹¹⁸ P{GD4194}v45743

v318038

PBac{fTRG00115.sfGFP-TVPTBF}VK00033

v342294

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD02015}attP40/CyO-GFP

More stocks available...

Genomic Clones (15)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (78)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequences

BDGP DGC clones

GH13089

Other clones

DPiM_GH13089

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0024371

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0024371

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35381

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Clavier et al., 2014, Korenjak et al., 2012, Sim et al., 2012)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: E2f2 CG1071

(Wortman, 1999.11)

Source for database merge of

Additional comments

Other Comments

E2f2 as part of the E2f-Rb complexes has a role in the down regulation of CycE during the gap phase of endocycling cells in normal development.

(Weng et al., 2003)

E2f2 mutants are viable, but females are partially sterile. Mutant follicle cells terminate endocycles normally, but then fail to confine DNA synthesis to sites of gene amplification and inappropriately begin genomic DNA replication (this ectopic DNA synthesis is not a continuation of the endocycle program).

(Cayirlioglu et al., 2001)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 53 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

35381

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O77051

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

X2J9B3

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0024371

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0024371

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

E2f2_1

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG1071

Flygut - An atlas of the Drosophila adult midgut

FBgn0024371

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

35381

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG1071

modMine - A data warehouse for the modENCODE project

FBgn0024371

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O77051

Linkouts

BioGRID - A database of protein and genetic interactions.

61336

DPiM - Drosophila Protein interaction map

FBgn0024371

DroID - A comprehensive database of gene and protein interactions.

FBgn0024371

DRSC - Results frm RNAi screens

FBgn0024371

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0024371

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG1071-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0024371

FlyMine - An integrated database for Drosophila genomics

FBgn0024371

Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution

Interactive Fly

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

35381

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

TGF-beta signaling pathway

MIST (genetic) - An integrated Molecular Interaction Database

E2f2

MIST (protein-protein) - An integrated Molecular Interaction Database

E2f2

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

CG1071

(Ni et al., 2011.7.19, Keleman et al., 2009.8.5, Perkins et al., 2009, Beall et al., 2007)

E2F

(Korenjak et al., 2012, Thacker et al., 2003)

E2F2

(Zhang et al., 2016, Gómez-Lamarca et al., 2014, Lim et al., 2013, modENCODE Consortium et al., 2010, Tanaka-Matakatsu et al., 2009, Wen et al., 2008, Wen et al., 2008, Beall et al., 2007, Doggett et al., 2007, Garcia et al., 2007, Georlette et al., 2007, Ullah et al., 2007, Parakati and Dimario, 2005, Heriche et al., 2003, Orian et al., 2003)

E2f

(Ashwal-Fluss et al., 2014, Ghavi-Helm et al., 2014, Singer et al., 2014, Bangi et al., 2011)

dE2F-2

(Huen et al., 1998)

dE2f2

(Bhaskar et al., 2014, Ji et al., 2012, Gordon and Du, 2011, Ji et al., 2006, Moon et al., 2005, Stevaux et al., 2005, Attwooll et al., 2004)

e2f2

(Miles et al., 2014, Miles et al., 2012, Rovani et al., 2012, Sim et al., 2012, Buttitta et al., 2010, Buttitta et al., 2007)

Name Synonyms

E2F transcription factor 2

(Davis et al., 2011, Terashima and Bownes, 2005, Huen et al., 1998)

Secondary FlyBase IDs

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (155)

Report Sections

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