Vanaso, l(3)dre1, l(3)04276, Van, dre1
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Gene model reviewed during 5.46
Shares 5' UTR with downstream gene.
3.5 (northern blot)
There is only one protein coding transcript and one polypeptide associated with this gene
800 (aa); 98 (kD observed); 88 (kD predicted)
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Comment: maternally deposited
dlt transcripts are detected at all developmental stages on northern blots. Expression is observed in many epithelial cells of ectodermal origin by in situ hybridization.
Protein is detected in the furrow canal region on the membrane of cellularizing embryos.
dlt protein is first detected at mitotic cycle 11 and is membrane-associated. It is present at the leading edge of the invaginating membranes during cellularization. After cellularization, it is exclusively localized to the basal area of the columnar epithelial cells. At all subsequent embryonic stages, it is membrane associated and is found in primary and some secondary epithelial cells including ectodermal cells, salivary glands, foregut, hindgut, and invaginating tracheal cells. It is also detected in some cells of the PNS.
GBrowse - Visual display of RNA-Seq signals
View Dmel\dlt in GBrowse 23-1.0
3-1.0
3-2.7
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Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for identity of: Van l(3)04276
Source for identity of: Vanaso CG32315
Source for merge of: dlt l(3)62Ba
The "dre1/dlt" complementation group corresponds to the CG32315 gene and not to CG12021. It was previously reported in FBrf0107610 that the relevant locus for the "dre1" (dlt) complementation group was CG12021, based on mapping and complementation data (shown in Figure 4. of FBrf0107610). However, it has recently been shown (FBrf0167484) that "dre1" is allelic to CG32315 rather than CG12021. Re-evaluation of the "dre1" mutation indicates that the chromosome carries a T516S amino acid change in CG12021 and a stop codon at position 865 in CG32315. Since the mutation in CG12021 is conservative, it may not underlie the loss of imaginal discs observed in "dre1" mutants, and instead, the "dre1" mutant phenotypes described in FBrf0107610 are likely due to the mutation in CG32315. This is also supported by the observation that the P{PatjEHHS} construct (Figure 4 of FBrf0107610) now fails to rescue the "dre1" mutation. In addition, the "dre1" mutation fails to complement an allele in CG32315 ("Van04276"), clearly implicating CG32315 as causing the lethality associated with "dre1". It remains possible that the "dre1" phenotypes shown in Figure 6 of FBrf0107610 may result from a combination of mutations in both genes. Overexpression of CG12021 causes a severe loss of epithelial polarity as shown in Figure 7. of FBrf0107610. FlyBase curator comment: the T516S amino acid change in CG12021 on the "dre1" chromosome is represented by the allele Patjdre1 and the stop codon at position 865 in CG32315 on the "dre1" chromosome is represented by the allele dltdre1.
The dlt phenotype maps to the CG32315 annotation, not the CG12021 annotation, as was reported previously (FBrf0107610).
Identification: as a female-specific quantitative trait locus affecting variation in olfactory avoidance response to benzaldehyde.
dlt is expressed exclusively in the third antennal segment.
Mutations cause pupal lethality with third instar larvae devoid of imaginal discs.
Recessive lethal mutations in this complementation group were identified by failure to complement the recessive lethality of Df(3L)R-R2.
FlyBase curator comment: The gene corresponding to genome annotation CG12021 was named " dlt:discs lost" in FBrf0107610. Subsequently the "dlt" phenotype was shown to map not to CG12021, but to the gene corresponding to genome annotation CG32315. Consequently the " dlt:discs lost" name was moved to CG32315, and the name of the gene for CG12021 was changed to "Patj". See Klaembt, 2003.10.31, personal communication to FlyBase
The gene is named "Vanaso" since segregation of putative alleles in Oregon and 2b strains causes additive genetic variance (V[[A]]) in olfactory avoidance behaviour ("naso" is a Spanish term for nose).