Gene Dmel\Dph5

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General Information

Symbol

Dmel\Dph5

Species

D. melanogaster

Name

Diphthamide methyltransferase

Annotation symbol

CG31289

Feature type

protein_coding_gene

FlyBase ID

FBgn0024558

Created / Updated

2003-12-01/2003-12-01

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

94B5-94B5

Sequence location

3R:18,407,749..18,408,950 [-]

Map ( GBrowse )

Summary Information

Automatically generated summary

See sections below for more information

The gene Diphthamide methyltransferase is referred to in FlyBase by the symbol Dph5 (CG31289, FBgn0024558). It has the cytological map location 94B5. Its sequence location is 3R:18407749..18408950. Its molecular function is described as diphthine synthase activity. It is involved in the biological process peptidyl-diphthamide biosynthetic process from peptidyl-histidine. 3 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

94B5-94B5

Limits computationally determined from genome sequence between P{PZ}l(3)rQ178^rQ178 and P{lacW}Dph5^L4910

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

94B4-94B5

(determined by in situ hybridisation)

(Spradling et al., 1999)

94B4-94B5

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Molecular Map Data

Gene Order (in direction of increasing cytology)

References

Gene Order (overall orientation not stated)

References

Gene Model & Products

Please see the GBrowse view of Dmel\Dph5 for information on other features

Comments on Gene Model

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

Dph5-RA

FBtr0084280

NM_079728

1132

281

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kD)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

Dph5-PA

FBpp0083673

31.6

281

5.21

NP_524452

AAN13913

Additional Polypeptide Data & Comments

Reported size (kD)

Comments

External Data

Linkouts

PANTHER - Protein classification by function, families, and pathways

•

FBgn0024558

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tetrapyrrole methylase (IPR000878)

Diphthine synthase (IPR004551)

Tetrapyrrole methylase, subdomain 2 (IPR014776)

Tetrapyrrole methylase, subdomain 1 (IPR014777)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Q8IN02

Maps to

Does NOT map to

Identified with

(Oliver, 2001.8.16)

LD12153

(Spradling et al., 1999, BDGP Project Members, 2000-)

Mapped Features & Mutations

Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

DEDB - Drosophila exon database: splicing graphs

•

10348

Crossreferences

Expression Data

FlyBase-Curated Data

Transcript and
Protein data

Please see the FlyBase Gene Expression Report for details of gene expression from the literature.

Summary of Transcript Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

Summary of Polypeptide Expression

Stage

Tissue/Position

Reference

Marker for

Subcellular Localization

CV Term

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0024558

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0024558

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0024558

FlyExpress - Embryonic expression images (BDGP data)

•

Show all FlyExpress processed images for Dph5

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | recessive

Dph5^L4910

Phenotype manifest in

Allele

Classical Alleles ( 2 )

For All Classical Alleles Show

Allele of Dph5	Class	Mutagen	Stocks	Known lesion
Dph5^EP3277		P-element activity	1	--
Dph5^L4910		P-element activity	1	--

Alleles Carried on Transgenic Constructs ( 1 )

For All Alleles Carried on Transgenic Constructs Show

Allele of Dph5	Class	Mutagen	Stocks	Known lesion
Dph5^GD14944		in vitro construct \| RNAi in vitro construct \| regulatory fusion	2	Yes

Aneuploid Aberrations

Useful deficiency

Df(3R)hh

Useful duplication

Dp(3;3)M95A⁺13

Not disrupted in

Df(3R)hhE23

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

Disrupted in

Df(3R)BSC34

(Andrade et al., 2003.5.20)

Df(3R)ED6093

(Ryder and Roote, 2004.11.10)

Df(3R)Exel6273

(Ryder, 2004.4.26)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD14944}

Insertions

Type of insertions

Name

Expression data

insertion of enhancer trap

P{lacW}Dph5^L4910

insertion of mobile activating element

P{EP}Dph5^EP3277

Related Comments

Please look at the allele reports for the complete phenotype data

Gene Ontology: Function, Process & Cellular Component ( 2 )

Molecular Function

CV term

Evidence

References

diphthine synthase activity

inferred from sequence or structural similarity

(Spradling et al., 1999)

inferred from sequence or structural similarity with SGD_LOCUS:DPH5; SGD:S0004162

(FlyBase Genome Annotators, 2002-2003)

Biological Process

CV term

Evidence

References

peptidyl-diphthamide biosynthetic process from peptidyl-histidine

inferred from sequence or structural similarity with SGD_LOCUS:DPH5; SGD:S0004162

(FlyBase Genome Annotators, 2002-2003)

Cellular Component

CV term

Evidence

References

Sequence Ontology: Class of Gene

protein_coding_gene

nuclear_gene

Interactions & Pathways

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

Dph5 allele

Gene

References

External Data

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

67617

Orthologs

Genome-wide drosophilid orthologs

Dana\GF16145

Dere\GG12504

Dgri\GH18052

Dmoj\GI10523

Dper\GL13679

Dpse\GA16151

Dsec\GM23636

Dsim\GD18446

Dvir\GJ24174

Dwil\GK11785

Dyak\Dph5

Curated drosophilid orthologs

Dpse\GA16151

Dyak\Dph5

Linkouts

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000003767
Anopheles gambiae (Ensembl)
ENSBTAP00000025326
Bos taurus (Ensembl)
ENSCAFP00000029626
Canis familiaris (Ensembl)
ENSCINP00000018685
Ciona intestinalis (Ensembl)
NEWSINFRUP00000166840
Takifugu rubripes (Ensembl)
ENSGALP00000008387
Gallus gallus (Ensembl)
ENSP00000251159
Homo sapiens (Ensembl)
ENSMMUP00000004971
Macaca mulatta (Ensembl)
ENSMODP00000004122
Monodelphis domestica (Ensembl)
ENSPTRP00000001738
Pan troglodytes (Ensembl)
ENSRNOP00000018367
Rattus norvegicus (Ensembl)
GSTENP00008282001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000029018
Xenopus tropicalis (Ensembl)
AT4G31790.2
Arabidopsis thaliana (Arabidopsis)
WBGene00025877
Caenorhabditis briggsae (Wormbase)
WBGene00007194
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-041114-85
Danio rerio (Zfin)
ZDB-GENE-041114-85
Danio rerio (Zfin)
DDB0169516
Dictyostelium discoideum (Dictybase)
MGI:1916990
Mus musculus (Informatics)
YLR172C
Saccharomyces cerevisiae (Yeastgenome)
SPCC576.14
Schizosaccharomyces pombe (Sanger)

Functional Complementation between Species

Inter-Species Misexpression Data

Produces phenotype in

Produces NO phenotype in

Stocks & Reagents

Stocks Listed in FlyBase ( 4 )

Szeged

P{EP}Dph5^EP3277

VDRC

v39090

w¹¹¹⁸; P{GD14944}v39090

v39091

w¹¹¹⁸; P{GD14944}v39091/CyO

Bloomington

10318

y¹ w¹¹¹⁸; P{lacW}Dph5^L4910/TM3, Ser¹

Genomic Clones ( 2 )

cDNA Clones ( 20 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

LD12153

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Affy Oligo

Linkouts

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0024558

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0024558

Antibody Information

Other Information

Discoverer

Etymology

Identification

Position Effect Variegation Data

Relationship to Other Genes

Source for database identity of

Source for database merge of

Additional comments

Source for split of: CG5275 Annotation CG5275 split into CG33107 and CG31289 (Dph5) in release 3 of the genome annotation.

(FlyBase Genome Annotators, 2002-2003)

Comments About Role

Comments About Molecular Function

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_079728

RefSeq (Proteins)

•

NP_524452

DDBJ / EMBL / GenBank

•

UniProtKB/TrEMBL

•

Q8IN02

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Tetrapyrrole methylase (IPR000878)

Diphthine synthase (IPR004551)

Tetrapyrrole methylase, subdomain 2 (IPR014776)

Tetrapyrrole methylase, subdomain 1 (IPR014777)

Linkouts

BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions

•

67617

DEDB - Drosophila exon database: splicing graphs

•

10348

DRSC - RNAi screening (Harvard): high-throughput cell culture data and design

•

FBgn0024558

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0024558

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

FBgn0024558

FlyExpress - Embryonic expression images (BDGP data)

•

FBgn0024558

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0024558

GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies

•

FBgn0024558

GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data

•

FBgn0024558

InParanoid orthologs - Eukaryotic orthologs

•

ENSANGP00000003767
Anopheles gambiae (Ensembl)
ENSBTAP00000025326
Bos taurus (Ensembl)
ENSCAFP00000029626
Canis familiaris (Ensembl)
ENSCINP00000018685
Ciona intestinalis (Ensembl)
NEWSINFRUP00000166840
Takifugu rubripes (Ensembl)
ENSGALP00000008387
Gallus gallus (Ensembl)
ENSP00000251159
Homo sapiens (Ensembl)
ENSMMUP00000004971
Macaca mulatta (Ensembl)
ENSMODP00000004122
Monodelphis domestica (Ensembl)
ENSPTRP00000001738
Pan troglodytes (Ensembl)
ENSRNOP00000018367
Rattus norvegicus (Ensembl)
GSTENP00008282001
Tetraodon nigroviridis (Ensembl)
ENSXETP00000029018
Xenopus tropicalis (Ensembl)
AT4G31790.2
Arabidopsis thaliana (Arabidopsis)
WBGene00025877
Caenorhabditis briggsae (Wormbase)
WBGene00007194
Caenorhabditis elegans (Wormbase)
ZDB-CDNA-041114-85
Danio rerio (Zfin)
ZDB-GENE-041114-85
Danio rerio (Zfin)
DDB0169516
Dictyostelium discoideum (Dictybase)
MGI:1916990
Mus musculus (Informatics)
YLR172C
Saccharomyces cerevisiae (Yeastgenome)
SPCC576.14
Schizosaccharomyces pombe (Sanger)

PANTHER - Protein classification by function, families, and pathways

•

FBgn0024558

University of Chicago Dev. Expression

•

FBgn0024558

Synonyms & Secondary IDs ( 7 )

Reported As

Symbol Synonym

BcDNA:LD12153

BcDNA.LD12153

(Tsang, 1999.6.19)

CG5275

(Oliver, 2001.8.16, )

CG31289

Dph5

l(3)L4910

(Beaton, 2000.1.31, Zhang and Broadie, 1999, BDGP Project Members, 1994-1999, )

Name Synonym

Diphthamide methyltransferase

(Spradling et al., 1999)

Secondary FlyBase IDs

FBgn0027877
FBgn0051289

References ( 17 )

Research paper

Spradling et al., 1999, Genetics 153(1): 135--177: The Berkeley Drosophila genome project gene disruption project. Single P-element insertions mutating 25% of vital Drosophila genes. [FBrf0111489]
Zhang and Broadie, 1999, Gene 233(1-2): 171--179: Cloning, mapping and tissue-specific expression of Drosophila clathrin-associated protein AP50 gene. [FBrf0111181]

Supplementary material

Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
Ryder and Roote, 2004.11.10, DrosDel deletions confirmed by PCR and genetic tests.: DrosDel deletions confirmed by PCR and genetic tests. [FBrf0179888]
Ryder, 2004.4.26, Exelixis CG deletion data.: Exelixis CG deletion data. [FBrf0184335]
Andrade et al., 2003.5.20, Isolation and Characterization of Df(3R)BSC34.: Isolation and Characterization of Df(3R)BSC34. [FBrf0159866]
Oliver, 2001.8.16, File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348).: File from B. Oliver containing information on ESTs expressed in the Drosophila testis, described in Andrews et al., 2000, Genome Res. 10(12): 2030-2043 (FBrf0132348). [FBrf0137492]
Beaton, 2000.1.31, BDGP P element Update.: BDGP P element Update. [FBrf0126832]

FlyBase analysis

FlyBase Genome Annotators, 2002-2003, Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome.: Changes affecting gene model number or type in releases 3.0 and 3.1 of the annotated D.melanogaster genome. [FBrf0148886]
FlyBase, 1992-, FlyBase curation: FlyBase curation. [FBrf0105495]

DNA/RNA sequence record

Tsang, 1999.6.19, GenBank/EMBL/DDBJ: AF160929: Drosophila melanogaster clone LD12153 BcDNA.LD12153 (BcDNA.LD12153) mRNA, complete cds. [FBrf0121516]

Computer file

Gene Disruption Project members, 2001-, [title not yet available]: [title not yet available] [FBrf0132177]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0125078]
BDGP Project Members, 1994-1999, Berkeley Drosophila Genome Project.: Berkeley Drosophila Genome Project. [FBrf0067338]

Automatic genome annotation

FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126705]
Find Enzymes, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0126664]