• Home
• Tools
  • BLAST
  • GBrowse
  • QueryBuilder
  • TermLink
  • ImageBrowse
  • Interactions Browser
  • Apollo
  • CytoSearch
  • QuickSearch
  • Batch Download
  • Coordinate Converter
  • Google™ FlyBase
  • Find A Person
  • Update An Address
  • Add A New Person
• Files
  • Downloads:
  • Precomputed files
  • Archived data
  • Releases FTP
  • Genomes FTP
  • Lists:
  • Transposons (Dmel)
  • Transposons (other)
  • Vectors & Constructs
  • Anatomical Terms
  • Map Conversion
• Species
  • Phylogeny
  • Synteny Table
  • Drosophilidae
  • Abbreviations
• Documents
  • About FlyBase
  • Reference Manual:
  • Sections
  • Contents
  • Nomenclature
  • Release Notes
• Resources
  • All resources
  • Interactive Fly
  • Textpresso for Fly
  • AAA site
  • BDGP
  • DHGP
  • DGRC
  • Model Organisms
  • Stock Collections:
  • Bloomington
  • DrosDel
  • Ehime
  • Exelixis (Harvard)
  • GDP (Baylor)
  • Kyoto
  • NIG-Fly
  • Szeged
  • Tucson
  • VDRC
• News
  • News
  • FlyBase Forum
  • FlyBase Positions
  • Meetings
  • Courses
  • Fly Board
  • White papers
  • Funding
  • bionet.dros
• Help
  • Google™ FlyBase
  • Site Map
  • FAQs
  • Report help
  • New to Flies
  • Pers. comm.
  • Author guidelines
  • Known Problems
  • Contact FlyBase
• Archives
  • Prior Home Page
  • R4.3 BLAST
  • R4.3 GBrowse
  • Older Archives

Gene Tni\Tni\piggyBac\T

General Information
Symbol	Tni\Tni\piggyBac\T	Species	T. ni
Name	piggyBac transposase	Annotation symbol
Feature type	engineered_foreign_gene	FlyBase ID	FBgn0024718
Created / Updated	2006-08-22/2006-08-22
Genomic Location
Chromosome (arm)		Recombination map
Cytogenetic map		Sequence location
Summary Information
Automatically generated summary See sections below for more information	The gene piggyBac transposase is referred to in FlyBase by the symbol Tni\piggyBac\T (FBgn0024718). It has not been localized to the genome sequence. Its molecular function is unknown. The biological processes in which it is involved are not known. 10 alleles are reported. No phenotypic data is available. It has no annotated transcripts.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References Gene Order (overall orientation not stated) References
Gene Model & Products
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa)
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts
Crossreferences
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AY196826
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL
Maps to
Does NOT map to
Identified with
Mapped Features & Mutations
Type Symbol & Location Additional Notes References
External Data
Linkouts
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024718
Alleles & Phenotypes
Summary of Allele Phenotypes
Phenotype manifest in Allele
Classical Alleles ( 0 )
For All Classical Alleles Show
Allele of Tni\piggyBac\T Class Mutagen Stocks Known lesion
Alleles Carried on Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Allele of Tni\piggyBac\T Class Mutagen Stocks Known lesion Tni\piggyBac\TTRL in vitro construct | deletion 0 Yes Tni\piggyBac\TUbi-p63E.PG in vitro construct | other in vitro construct | regulatory fusion 0 Yes Tni\piggyBac\TUbi.PB in vitro construct | regulatory fusion 0 Yes Tni\piggyBac\TWC in vitro construct | deletion 0 Yes Tni\piggyBac\Ths.PB in vitro construct | regulatory fusion 0 Yes Tni\piggyBac\Ths.PBb in vitro construct | regulatory fusion 0 Yes Tni\piggyBac\Ths.PH in vitro construct | deletion in vitro construct | regulatory fusion 0 Yes Tni\piggyBac\Ths.PT in vitro construct | regulatory fusion 1 Yes Tni\piggyBac\TΔSac in vitro construct | other 0 Yes Tni\piggyBac\TαTub84B.PH in vitro construct | regulatory fusion in vitro construct | coding region fusion 2 Yes
Aneuploid Aberrations
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data
Insertions
	Type of insertions Name Expression data
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 0 )
Molecular Function
CV term Evidence References
Biological Process
CV term Evidence References
Cellular Component
CV term Evidence References
Sequence Ontology: Class of Gene
	engineered_foreign_gene   transposable_element_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions Tni\piggyBac\T allele Gene References
External Data
Linkouts
Orthologs
Genome-wide drosophilid orthologs
Curated drosophilid orthologs
Linkouts
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 3 )
Bloomington	8285 w1118; CyO, P{Tub-PBac\T}2/wgSp-1 8283 w1118; CyO, P{FRT(w+)Tub-PBac\T}2/wgSp-1 8284 y1 w1118; CyO, P{FRT(w+)Hsp70-PBac\T}2/wgSp-1
Genomic Clones ( 0 )
cDNA Clones ( 0 )
cDNA Clones, Fully Sequenced
BDGP DGC clones
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones
Other clones
RNAi & Array Information
Affy Oligo
Linkouts
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of
Source for database merge of
Encoded by	Tni\piggyBac
Additional comments
Comments About Role
Comments About Molecular Function
Other Comments
Foreign Gene Data
	Foreign sequence; species == Trichoplusia ni; gene == 'piggyBac transposase'.
External Crossreferences & Linkouts
Sequence Crossreferences
	DDBJ / EMBL / GenBank • AY196826
Other Crossreferences
Linkouts
	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024718
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	pBACtrans (Quinones-Coello et al., 2007, Buszczak et al., 2007) Tni\piggyBac\T   unnamed (Thibault et al., 2004)
Name Synonym	piggyBac transposase
Secondary FlyBase IDs
References ( 17 )
Research paper	Buszczak et al., 2007, Genetics 175(3): 1505--1531 The Carnegie protein trap library: A versatile tool for Drosophila developmental studies. [FBrf0200345] Quinones-Coello et al., 2007, Genetics 175(3): 1089--1104 Exploring strategies for protein trapping in Drosophila. [FBrf0200349] Bonin, 2004, Genetics 167(4): 1801--1811 A piggyBac transposon gene trap for the analysis of gene expression and function in Drosophila. [FBrf0179794] Thibault et al., 2004, Nature Genetics 36(3): 283--287 A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. [FBrf0175002] Hacker et al., 2003, Proc. Natl. Acad. Sci. USA 100(13): 7720--7725 piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. [FBrf0159706] Horn et al., 2003, Genetics 163(2): 647--661 piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. [FBrf0158985] Kambris et al., 2003, EMBO Rpts 4(1): 64--69 DmMyD88 controls dorsoventral patterning of the Drosophila embryo. [FBrf0155743] Griswold et al., 2002, D. I. S. 85: 129--132 A toolkit for transformation and mutagenesis in Drosophila using piggyBac. [FBrf0179717] Horn and Wimmer, 2000, Dev. Genes Evol. 210(12): 630--637 A versatile vector set for animal transgenesis. [FBrf0134553] Handler and Harrell, 1999, Insect Molec. Biol. 8(4): 449--457 Germline transformation of Drosophila melanogaster with the piggyBac transposon vector. [FBrf0123037] Lobo et al., 1999, Molec. gen. Genet. 261(4-5): 803--810 Transposition of the piggyBac element in embryos of Drosophila melanogaster, Aedes aegypti and Trichoplusia ni. [FBrf0108885]
Personal communication to FlyBase	Parks, 2004.5.12, Flip-out piggyBac transposase constructs and insertions. Flip-out piggyBac transposase constructs and insertions. [FBrf0178854] Ring and Garza, 2003.6.10, PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. PBac Constructs from the Project 'Genome Wide Dispersal of Half-P Elements in Drosophila Using PiggyBac. [FBrf0159884]
Abstract	Thibault, 2002, A. Dros. Res. Conf. 43: 969C The piggyBac Transposon Complements P as a Tool for Large Scale Forward Mutagenesis. [FBrf0146431] Shirk et al., 1998, Abstr. Keystone Symp., Genet. Manip. Insects, 1998: 35 A piggyBac transposon based gene vector system for insects: construction and functional testing in insects from three orders. [FBrf0100245]
FlyBase analysis	FlyBase Curators, 1992-, Summary information describing the origin of foreign genes and tags in FlyBase. Summary information describing the origin of foreign genes and tags in FlyBase. [FBrf0199194]
DNA/RNA sequence record	Griswold, 2002.12.13, GenBank/EMBL/DDBJ: AY196826 PiggyBac ubiquitin-transposase P replacement vector EP3005, complete sequence. [FBrf0171719]