FB2025_01 , released February 20, 2025
Gene: Dmel\Flo1
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General Information
Symbol
Dmel\Flo1
Species
D. melanogaster
Name
Flotillin 1
Annotation Symbol
CG8200
Feature Type
protein_coding_gene
FlyBase ID
FBgn0024754
Gene Model Status
Current
Stock Availability
9 publicly available
Gene Summary
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. (UniProt, O61491)
Contribute a Gene Snapshot for this gene.
All Summaries
Also Known As

Flo, flotillin, Flo-1, reggie-2

Key Links
Genomic Location
Cytogenetic map
52A13-52A13
Sequence location
Recombination map
2-74
RefSeq locus
NT_033778 REGION:15566271..15569404
Sequence
Get Decorated FASTA
Genomic Maps
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
NCBI
UCSC
Ensembl
PopFly
Function
Gene Ontology (GO) Annotations (11 terms)
Molecular Function (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
enables protease binding
inferred from biological aspect of ancestor with PANTHER:PTN008551622
(GO Reference Genome Project, 2011-)
enables structural molecule activity
non-traceable author statement
(Galbiati et al., 1998)
Biological Process (5 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (5 terms)
CV Term
Evidence
References
involved_in positive regulation of cell junction assembly
inferred from biological aspect of ancestor with PANTHER:PTN001013417
(GO Reference Genome Project, 2011-)
involved_in positive regulation of cell-cell adhesion mediated by cadherin
inferred from biological aspect of ancestor with PANTHER:PTN001013417
(GO Reference Genome Project, 2011-)
involved_in positive regulation of endocytosis
inferred from biological aspect of ancestor with PANTHER:PTN001013417
(GO Reference Genome Project, 2011-)
involved_in protein localization to plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN008551622
(GO Reference Genome Project, 2011-)
involved_in regulation of receptor internalization
inferred from biological aspect of ancestor with PANTHER:PTN001013417
(GO Reference Genome Project, 2011-)
Cellular Component (4 terms)
Terms Based on Experimental Evidence (1 term)
CV Term
Evidence
References
located_in cytoplasm
inferred from high throughput direct assay
(Lee et al., 2014)
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
is_active_in cytoplasmic vesicle
inferred from biological aspect of ancestor with PANTHER:PTN001013417
(GO Reference Genome Project, 2011-)
part_of flotillin complex
non-traceable author statement
(Galbiati et al., 1998)
inferred from biological aspect of ancestor with PANTHER:PTN001013417
(GO Reference Genome Project, 2011-)
is_active_in plasma membrane
inferred from biological aspect of ancestor with PANTHER:PTN000351686
(GO Reference Genome Project, 2011-)
Gene Group (FlyBase)
Protein Family (UniProt)
Belongs to the band 7/mec-2 family. Flotillin subfamily. (O61491)
Protein Signatures (InterPro)
Summaries
Gene Group (FlyBase)
FLOTILLINS -
Flotillins are integral membrane proteins associated with caveolae. (Adapted from FBrf0102313).
Protein Function (UniProtKB)
May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles.
(UniProt, O61491)
Gene Model and Products
Number of Transcripts
2
Number of Unique Polypeptides
2

Please see the JBrowse view of Dmel\Flo1 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O61491)

If you don't see a structure in the viewer, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures
Crossreferences
Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.50

Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
Flo1-RA
FBtr0087351
NM_058010
1818
426
Flo1-RB
FBtr0087352
NM_166102
1830
430
Additional Transcript Data and Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
Flo1-PA
FBpp0086484
47.1
426
5.35
O61491
NP_477358
AAF58120
Flo1-PB
FBpp0086485
47.5
430
5.35
NP_725476
AAF58121
Polypeptides with Identical Sequences

None of the polypeptides share 100% sequence identity.

Additional Polypeptide Data and Comments
Reported size (kDa)

426 (aa); 47 (kD predicted)

(Galbiati et al., 1998)
Comments
External Data
Subunit Structure (UniProtKB)

Heterooligomeric complex of flotillins 1 and 2 and caveolins 1 and 2.

(UniProt, O61491)
Crossreferences
InterPro - A database of protein families, domains and functional sites
Linkouts
Sequences Consistent with the Gene Model
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\Flo1 using the Feature Mapper tool.

External Data
Crossreferences
Linkouts
Expression Data
Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

-0.21

Transcript Expression
Polypeptide Expression
immunolocalization
Stage
Tissue/Position (including subcellular localization)
Reference
embryonic stage 16
larval ventral nerve cord | restricted
(Hoehne et al., 2005)
adult stage
adult optic lobe | restricted
(Hoehne et al., 2005)
second optic chiasma | restricted
(Hoehne et al., 2005)
first optic chiasma | restricted
(Hoehne et al., 2005)
pedunculus of adult mushroom body
(Hoehne et al., 2005)
neuron | subset
(Hoehne et al., 2005)
medullary tangential neuron | faint | subset
(Hoehne et al., 2005)
mass spectroscopy
Stage
Tissue/Position (including subcellular localization)
Reference
adult stage
adult head
(Aradska et al., 2015)
day 7 of adulthood -- day 49 of adulthood
adult heart
(Cammarato et al., 2011)
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
located_in cytoplasm
inferred from high throughput direct assay
(Lee et al., 2014)
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\Flo1 in JBrowse
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Bulk Downloads
RNA-Seq RPKM values for all genes
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 4-6
  • Stages(s) 7-8
  • Stages(s) 9-10
  • Stages(s) 11-12
  • Stages(s) 13-16
FlyBase Wiki
Image Based Resources
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 3 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 10 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of Flo1
Transgenic constructs containing regulatory region of Flo1
Aberrations (Deficiencies and Duplications) ( 0 )
Inferred from experimentation ( 0 )
Inferred from location ( 8 )
Variants
Variant Molecular Consequences
Alleles Representing Disease-Implicated Variants
Phenotypes
Orthologs
Downloads
Download All DIOPT Orthologs
Human Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Homo sapiens (Human) (8)
Hsap\FLOT1
14 of 14
Yes
Yes
2  
Hsap\FLOT2
4 of 14
No
No
Hsap\LAMB1
1 of 14
No
No
Hsap\NPHS2
1 of 14
No
No
Hsap\STOM
1 of 14
No
No
1  
Hsap\STOML1
1 of 14
No
No
Hsap\STOML2
1 of 14
No
No
2  
Hsap\STOML3
1 of 14
No
No
Model Organism Orthologs (via DIOPT v9.1)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Source
Compara
Domainoid
eggNOG
Hieranoid
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Complementation?
Transgene?
Rattus norvegicus (Norway rat) (4)
Rnor\Flot1
14 of 14
Yes
Yes
Rnor\Flot2
4 of 14
No
No
Rnor\Nphs2
1 of 14
No
No
Rnor\Stoml2
1 of 14
No
No
Mus musculus (laboratory mouse) (4)
Mmus\Flot1
14 of 14
Yes
Yes
Mmus\Flot2
3 of 14
No
No
Mmus\Nphs2
1 of 14
No
No
Mmus\Stoml2
1 of 14
No
No
Xenopus tropicalis (Western clawed frog) (6)
Xtro\flot1
8 of 13
Yes
Yes
Xtro\LOC100490322
3 of 13
No
No
Xtro\flot2
2 of 13
No
No
Xtro\LOC100493429
1 of 13
No
No
Xtro\stoml1
1 of 13
No
No
Xtro\stom
1 of 13
No
No
Danio rerio (Zebrafish) (7)
Drer\flot1b
14 of 14
Yes
Yes
Drer\flot1a
13 of 14
No
Yes
Drer\flot2a
4 of 14
No
No
Drer\flot2b
4 of 14
No
No
Drer\stom
1 of 14
No
No
Drer\stoml2
1 of 14
No
No
Drer\zgc:112408
1 of 14
No
No
Caenorhabditis elegans (Nematode, roundworm) (11)
Cele\epi-1
1 of 14
Yes
No
Cele\mec-2
1 of 14
Yes
No
Cele\stl-1
1 of 14
Yes
No
Cele\sto-1
1 of 14
Yes
No
Cele\sto-2
1 of 14
Yes
No
Cele\sto-3
1 of 14
Yes
No
Cele\sto-4
1 of 14
Yes
No
Cele\sto-5
1 of 14
Yes
No
Cele\sto-6
1 of 14
Yes
No
Cele\unc-1
1 of 14
Yes
No
Cele\unc-24
1 of 14
Yes
No
Anopheles gambiae (African malaria mosquito) (7)
Agam\AgaP_AGAP007494
12 of 12
Yes
Yes
Agam\AgaP_AGAP003789
3 of 12
No
No
Agam\AgaP_AGAP012547
3 of 12
No
No
Agam\AgaP_AGAP012884
3 of 12
No
Yes
Agam\AgaP_AGAP003352
1 of 12
No
No
Agam\AgaP_AGAP009439
1 of 12
No
No
Agam\BND7A_ANOGA
1 of 12
No
No
Arabidopsis thaliana (thale-cress) (9)
Atha\AT5G25260
6 of 13
Yes
Yes
Atha\AT5G64870
6 of 13
Yes
Yes
Atha\FLOT1
6 of 13
Yes
Yes
Atha\AT1G69840
1 of 13
No
No
Atha\AT4G27585
1 of 13
No
No
Atha\AT5G51570
1 of 13
No
No
Atha\AT5G54100
1 of 13
No
No
Atha\HIR1
1 of 13
No
No
Atha\HIR2
1 of 13
No
No
Saccharomyces cerevisiae (Brewer's yeast) (0)
Schizosaccharomyces pombe (Fission yeast) (1)
Spom\SPBC16G5.07c
1 of 12
Yes
No
Escherichia coli (enterobacterium) (2)
Ecol\yqiK
3 of 11
Yes
Yes
Ecol\qmcA
1 of 11
No
No
Other Organism Orthologs (via OrthoDB)
Data provided directly from OrthoDB:Flo1. Refer to their site for version information.
Paralogs
Downloads
Download All DIOPT Paralogs
Paralogs (via DIOPT v9.1)
Gene Symbol
Score
Source
Compara
Domainoid
eggNOG
Homologene
Inparanoid
OMA
OrthoDB
OrthoFinder
OrthoInspector
orthoMCL
Panther
Phylome
SonicParanoid
Alignment
Drosophila melanogaster (Fruit fly) (8)
Flo2
7 of 13
CG14644
2 of 13
CG14736
1 of 13
CG31358
1 of 13
CG33253
1 of 13
CG42540
1 of 13
Mec2
1 of 13
Stoml2
1 of 13
Human Disease Associations
FlyBase Human Disease Model Reports
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Evidence
    References
    Potential Models Based on Orthology ( 0 )
    Human Ortholog
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Allele
    Disease
    Interaction
    References
    Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)
    Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
    Homo sapiens (Human)
    Gene name
    Score
    OMIM
    OMIM Phenotype
    DO term
    Complementation?
    Transgene?
    FLOT1; flotillin 1
    14 of 14
    606998
         
        FLOT2; flotillin 2
        4 of 14
        131560
            Functional Complementation Data
            Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
            Interactions
            Summary of Physical Interactions
            esyN Network Diagram
            Show neighbor-neighbor interactions:
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            Select Layout:
            Legend:
            Protein
            RNA
            Selected Interactor(s)
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please see the Physical Interaction reports below for full details
            protein-protein
            Physical Interaction
            Assay
            References
            Flo1 - Flo2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Flo1 - Ist1
            experimental knowledge based
            (Guruharsha et al., 2011)
            Flo1 - Vps2
            experimental knowledge based
            (Guruharsha et al., 2011)
            Flo1 - Vps60
            experimental knowledge based
            (Guruharsha et al., 2011)
            Flo1 - chico
            experimental knowledge based
            (Guruharsha et al., 2011)
            Flo1 - cmb
            experimental knowledge based
            (Guruharsha et al., 2011)
            Summary of Genetic Interactions
            esyN Network Diagram
            Show/hide secondary interactors 
            (data from AllianceMine provided by esyN)
            esyN Network Key:
            Suppression
            Enhancement
            Other Interaction Browsers
            View in FlyBase Interactions Browser   View in MIST tool (external link)
            Please look at the allele data for full details of the genetic interactions
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Starting gene(s)
            Interaction type
            Interacting gene(s)
            Reference
            Flo2
            enhanceable
            Flo1
            (Hoehne et al., 2005)
            External Data
            Subunit Structure (UniProtKB)
            Heterooligomeric complex of flotillins 1 and 2 and caveolins 1 and 2.
            (UniProt, O61491 )
            Linkouts
            BioGRID - A database of protein and genetic interactions.
            DroID - A comprehensive database of gene and protein interactions.
            MIST (protein-protein) - An integrated Molecular Interaction Database
            Pathways
            Signaling Pathways (FlyBase)
            Metabolic Pathways
            FlyBase
            External Links
            External Data
            Linkouts
            Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
            Class of Gene
            Genomic Location and Detailed Mapping Data
            Chromosome (arm)
            2R
            Recombination map
            2-74
            Cytogenetic map
            52A13-52A13
            Sequence location
            FlyBase Computed Cytological Location
            Cytogenetic map
            Evidence for location
            52A13-52A13
            Limits computationally determined from genome sequence between P{lacW}Vha36k07207 and P{lacW}l(2)k05713k05713
            Experimentally Determined Cytological Location
            Cytogenetic map
            Notes
            References
            52B1-52B2
            (determined by in situ hybridisation)
            (Galbiati et al., 1998)
            Experimentally Determined Recombination Data
            Location

            2-74

            (FlyBase, 2016)

            2-78.7

            (Comeron et al., 2012)
            Left of (cM)
            Right of (cM)
            Notes
            Stocks and Reagents
            Stocks (9)
            Genomic Clones (10)
             

            Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

            cDNA Clones (77)
             

            Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

            cDNA clones, fully sequenced
            BDGP DGC clones
            Other clones
            Drosophila Genomics Resource Center cDNA clones

            For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

            cDNA Clones, End Sequenced (ESTs)
            BDGP DGC clones
            Other clones
            RNAi and Array Information
            Linkouts
            DRSC - Results frm RNAi screens
            Antibody Information
            Laboratory Generated Antibodies
             

            polyclonal

            (Hoehne et al., 2005)
            Commercially Available Antibodies
             
            Cell Line Information
            Publicly Available Cell Lines
             
              Other Stable Cell Lines
               
                Other Comments

                Promoter structures of Flo abut to or overlap with the 3'UTR of Cdk5. Overall orientation not stated: Cdk5+ Flo+

                (Hoehne et al., 1999)

                Flo has been cloned and sequenced. Identified on the basis of similarity to mammalian flotillin-1 and flotillin-2/ESA.

                (Galbiati et al., 1998)

                Identification: cDNA screen for secreted and transmembrane proteins expressed during embryogenesis.

                (Kopczynski et al., 1998)
                Relationship to Other Genes
                Source for database merge of
                Additional comments
                Nomenclature History
                Source for database identify of

                Source for identity of: Flo-1 Flo

                (Galbiati et al., 1998)

                Source for identity of: Flo1 Flo-1

                (FlyBase, 2009-)
                Nomenclature comments
                Etymology
                Synonyms and Secondary IDs (21)
                Datasets (0)
                Study focus (0)
                Experimental Role
                Project
                Project Type
                Title
                Study result (0)
                Result
                Result Type
                Title
                External Crossreferences and Linkouts ( 48 )
                Sequence Crossreferences
                NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
                GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
                GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
                RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
                UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
                UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
                Other crossreferences
                AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
                BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
                DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
                EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
                FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
                FlyMine - An integrated database for Drosophila genomics
                InterPro - A database of protein families, domains and functional sites
                KEGG Genes - Molecular building blocks of life in the genomic space.
                MARRVEL_MODEL - MARRVEL (model organism gene)
                Linkouts
                BioGRID - A database of protein and genetic interactions.
                Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
                DroID - A comprehensive database of gene and protein interactions.
                DRSC - Results frm RNAi screens
                FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
                FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
                Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
                Flygut - An atlas of the Drosophila adult midgut
                FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
                iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)
                MIST (protein-protein) - An integrated Molecular Interaction Database
                Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.
                References (72)