fmi, flamingo, CT20776
The transcript designation "stan-RA" and protein designation "stan-PA" have been used for different isoforms in different releases.
gene_with_stop_codon_read_through ; SO:0000697
Stop-codon suppression (UGA) postulated; FBrf0216884
Gene model reviewed during 5.44
Annotated transcripts do not represent all possible combinations of alternative exons and/or alternative promoters.
Gene model reviewed during 5.49
Alternative translation stop created by use of multiphasic reading frames within coding region.
>12 (northern blot)
Interacts with ATP6AP2 (via N-terminus).
Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\stan using the Feature Mapper tool.
stan is found in the region of the developing sex comb in the distal transverse row bristle and sex combs and to a lesser extent on the adjacent transverse row bristles.
stan is strongly expressed in all sensory cell bodies and in sensory and motor axons from early stage 13 through to late embryogenesis. It is also expressed in all primary tracheal branches including the dorsal trunk, dorsal branch, transverse connective, ganglionic branch, lateral trunk, and spiracular branch. Expression is restricted to the lateral and apical (luminal) surfaces of the tracheal cells except for the posterior lateral trunk where it is found over the whole tracheal surface.
stan protein is detected in the CNS and PNS of embryos at 18h AEL. In the CNS, it is detected in the motor axons that innervate the body wall muscles (hypodermal) and at the presynaptic sites of the neuromuscular junction. It is also detected in the nerve roots that exit the ventral nerve cord. Western blot analyses indicates that it is expressed in the larval CNS.
At 24 hr APF stan protein is strongly in the growth cones of photoreceptor cells R1-R6, and weakly expressed in neuronal cell bodies in the lamina. At 30 hr APF, stan is expressed unevenly in the growth cones of photoreceptor cells R1-R6 in the lamina plexus. By 46 hr APF, stan can no longer be detected in the growth cones or axons of photoreceptors. This expression pattern coincides with growth cone target selection. stan protein is also transiently expressed in photoreceptor cell R8 axons as they enter the optic lobe.
In third instar larvae, protein is strongly expressed in the lamina plexus where R1-R6 axons terminate and in the area of the medulla in which R7 and R8 axons terminate. Expression in photoreceptor cell axons is restricted to the growth cones. Expression was also observed in the lobula that correlates with the terminations of medullary cortical neurons. Expression in the medulla and lamina persists through pupal development with increased staining of lamina and medulla cortical neurons observed.
Between 0 and 4 hours after puparium formation protein is evenly distributed at the cell surface of apical sheath cells and does not display polarized localization.
GBrowse - Visual display of RNA-Seq signalsView Dmel\stan in GBrowse 2
Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete
Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.
Source for merge of: stan fmi
Clonal analysis indicates that the ds/ft system and the stan/fz system act independently to confer planar cell polarity in the adult abdomen; each system confers and propagates polarity and can do so in the absence of the other.
dsRNA has been made from templates generated with primers directed against this gene. stan RNAi results in overextension of ddaD and ddaE dendrites.
Identification: 1 allele of stan have been identified in a screen to isolate genes required for normal neuronal morphogenesis in larval mushroom body neurons.
stan appears to facilitate competitive interactions between adjacent R8 axons to ensure their correct spacing, which suggests a general role in establishing non-overlapping dendritic fields. stan may also promote the formation of stable connections between R8 axons and their target cells.
in and fy are needed for cells to respond to pk and stan. Genetic analysis is not consistent with fz-like class of genes fz, pk, Vang, stan and dsh acting simply as positive or negative regulators of in and fy.
Mutations in stan alter the polarity of cuticular structures in all regions of the adult body, affecting epidermal hairs, sensory bristles and ommatidia.
Identification: identified as a mutant in which photoreceptor axons make aberrant projection patterns in mutant clones in the eye.
stan has an essential role in the formation of axon tracts, and controls planar cell polarity at epithelial cell-cell boundaries.