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General Information
Symbol
Dmel\p38b
Species
D. melanogaster
Name
p38b MAP kinase
Annotation Symbol
CG7393
Feature Type
FlyBase ID
FBgn0024846
Gene Model Status
Stock Availability
Enzyme Name (EC)
Mitogen-activated protein kinase (2.7.11.24)
Gene Summary
p38b MAP kinase (p38b) encodes the serine/threonine kinase that constitutes MAP kinase signaling cascades. It is phosphorylated by MAPK kinases and phosphorylates various substrates that play roles in stress responses, immune responses and differentiation. [Date last reviewed: 2019-03-14] (FlyBase Gene Snapshot)
Also Known As

p38, D-p38b, p38β, p38Kb, p38 MAPK

Key Links
Genomic Location
Cytogenetic map
Sequence location
Recombination map
2-49
RefSeq locus
NT_033779 REGION:13780689..13782611
Sequence
Other Genome Views
The following external sites may use different assemblies or annotations than FlyBase.
Function
GO Summary Ribbons
Gene Ontology (GO) Annotations (35 terms)
Molecular Function (3 terms)
Terms Based on Experimental Evidence (2 terms)
CV Term
Evidence
References
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN001934972
(assigned by GO_Central )
non-traceable author statement
inferred from biological aspect of ancestor with PANTHER:PTN000622075
(assigned by GO_Central )
non-traceable author statement
(assigned by UniProt )
Biological Process (30 terms)
Terms Based on Experimental Evidence (28 terms)
CV Term
Evidence
References
inferred from direct assay
involved_in circadian rhythm
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:p38a; FB:FBgn0015765
inferred from direct assay
(assigned by UniProt )
involved_in immune response
inferred from mutant phenotype
inferred from mutant phenotype
involved_in p38MAPK cascade
inferred from direct assay
inferred from genetic interaction with FLYBASE:p38a; FB:FBgn0015765
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:p38a; FB:FBgn0015765
inferred from direct assay
inferred from direct assay
(assigned by UniProt )
inferred from direct assay
inferred from mutant phenotype
inferred from direct assay
involved_in response to heat
inferred from mutant phenotype
inferred from mutant phenotype
inferred from genetic interaction with FLYBASE:Atf-2; FB:FBgn0265193
inferred from mutant phenotype
inferred from mutant phenotype
Terms Based on Predictions or Assertions (3 terms)
CV Term
Evidence
References
inferred from biological aspect of ancestor with PANTHER:PTN000622075
(assigned by GO_Central )
involved_in MAPK cascade
non-traceable author statement
inferred from sequence or structural similarity with UniProtKB:P47811
Cellular Component (2 terms)
Terms Based on Experimental Evidence (0 terms)
Terms Based on Predictions or Assertions (2 terms)
CV Term
Evidence
References
is_active_in cytoplasm
inferred from biological aspect of ancestor with PANTHER:PTN000622075
(assigned by GO_Central )
is_active_in nucleus
inferred from biological aspect of ancestor with PANTHER:PTN000622075
(assigned by GO_Central )
Protein Family (UniProt)
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. (O61443)
Catalytic Activity (EC)
Experimental Evidence
ATP + a protein = ADP + a phosphoprotein (2.7.11.24)
Predictions / Assertions
ATP + a protein = ADP + a phosphoprotein (2.7.11.24)
Summaries
Gene Snapshot
p38b MAP kinase (p38b) encodes the serine/threonine kinase that constitutes MAP kinase signaling cascades. It is phosphorylated by MAPK kinases and phosphorylates various substrates that play roles in stress responses, immune responses and differentiation. [Date last reviewed: 2019-03-14]
Gene Group (FlyBase)
CONVENTIONAL MITOGEN ACTIVATED PROTEIN KINASES -
Conventional Mitogen-activated protein kinases (MAPK) are proline-directed protein serine/threonine kinases that are components of the MAPK signaling cascade. MAPKs are activated by dual phosphorylation on Thr and Tyr residues within a conserved Thr-X-Tyr motif located in the activation loop by MAPK kinases. (Adapted from PMID:21372320).
Pathway (FlyBase)
Negative Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Protein Function (UniProtKB)
Kinase involved in dpp signal transduction pathway in the process of wing morphogenesis when the levels of dpp are enhanced or inhibited. May down-regulate insect immunity gene expression after prolonged infection.
(UniProt, O61443)
Summary (Interactive Fly)

a MAP kinase involved in transforming growth factor beta superfamily signal transduction in Drosophila wing morphogenesis - regulates Hippo signaling in ovarian follicle cells and the wing disc

Gene Model and Products
Number of Transcripts
1
Number of Unique Polypeptides
1

Please see the JBrowse view of Dmel\p38b for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)
Isoform displayed:
Pfam protein domains
InterPro name
classification
start
end
Protein Domains (via SMART)
Isoform displayed:
SMART protein domains
InterPro name
classification
start
end
Structure New Section
Protein 3D structure   (Predicted by AlphaFold)   (AlphaFold entry O61443)

If you don't see the viewer to the right, refresh your browser.
Model Confidence:
  • Very high (pLDDT > 90)
  • Confident (90 > pLDDT > 70)
  • Low (70 > pLDDT > 50)
  • Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Comments on Gene Model

Gene model reviewed during 5.40

Gene model reviewed during 5.52

Sequence Ontology: Class of Gene
Transcript Data
Annotated Transcripts
Name
FlyBase ID
RefSeq ID
Length (nt)
Assoc. CDS (aa)
FBtr0080534
1821
365
Additional Transcript Data and Comments
Reported size (kB)

1.143 (longest cDNA)

Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name
FlyBase ID
Predicted MW (kDa)
Length (aa)
Theoretical pI
UniProt
RefSeq ID
GenBank
FBpp0080111
42.1
365
6.45
Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments
Reported size (kDa)
Comments
External Data
Post Translational Modification

Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

(UniProt, O61443)
Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

(UniProt, O61443)
Linkouts
Sequences Consistent with the Gene Model
Nucleotide / Polypeptide Records
 
Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\p38b using the Feature Mapper tool.

External Data
Crossreferences
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
Linkouts
Expression Data
Expression Summary Ribbons
Colored tiles in ribbon indicate that the Fly Cell Atlas project found the gene expressed in that cell type. Darker colors mean that more cells of that cell type express the gene:
 low
high 
Colorless tiles indicate that there is no scRNAseq data for the gene in that cell type.
Colored tiles in ribbon indicate that expression data (RNA and/or protein) has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.
Colored tiles in the ribbon indicate the average RNA expression level of the gene at the indicated stages:
 low
high 
as determined by RNA-seq (RPKM) using whole organism samples modENCODE, Brown et al., 2014. For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.
Transcript Expression
Polypeptide Expression
Additional Descriptive Data
Marker for
 
Subcellular Localization
CV Term
Evidence
References
Expression Deduced from Reporters
High-Throughput Expression Data
Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\p38b in GBrowse 2
RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region
Reference
See Gelbart and Emmert, 2013 for analysis details and data files for all genes.
Developmental Proteome: Life Cycle
Developmental Proteome: Embryogenesis
External Data and Images
Linkouts
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
Images
FlyExpress - Embryonic expression images (BDGP data)
  • Stages(s) 1-3
  • Stages(s) 11-12
  • Stages(s) 13-16
Alleles, Insertions, Transgenic Constructs, and Aberrations
Classical and Insertion Alleles ( 12 )
For All Classical and Insertion Alleles Show
 
Other relevant insertions
Transgenic Constructs ( 25 )
For All Alleles Carried on Transgenic Constructs Show
Transgenic constructs containing/affecting coding region of p38b
Transgenic constructs containing regulatory region of p38b
Aberrations (Deficiencies and Duplications) ( 3 )
Inferred from experimentation ( 3 )
Inferred from location ( 0 )
Alleles Representing Disease-Implicated Variants
Phenotypes
For more details about a specific phenotype click on the relevant allele symbol.
Lethality
Allele
Sterility
Allele
Other Phenotypes
Allele
Phenotype manifest in
Allele
Orthologs
Human Orthologs (via DIOPT v8.0)
Homo sapiens (Human) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
0  
12 of 15
No
Yes
6 of 15
No
Yes
1  
5 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2  
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Model Organism Orthologs (via DIOPT v8.0)
Mus musculus (laboratory mouse) (10)
Species\Gene Symbol
Score
Best Score
Best Reverse Score
Alignment
Complementation?
Transgene?
15 of 15
Yes
Yes
12 of 15
No
Yes
6 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Rattus norvegicus (Norway rat) (11)
13 of 13
Yes
Yes
10 of 13
No
No
6 of 13
No
Yes
5 of 13
No
Yes
2 of 13
No
Yes
2 of 13
No
No
2 of 13
No
Yes
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
1 of 13
No
No
Xenopus tropicalis (Western clawed frog) (10)
11 of 12
Yes
Yes
8 of 12
No
Yes
5 of 12
No
Yes
4 of 12
No
Yes
2 of 12
No
No
2 of 12
No
Yes
2 of 12
No
Yes
1 of 12
No
No
1 of 12
No
No
1 of 12
No
No
Danio rerio (Zebrafish) (13)
14 of 15
Yes
Yes
13 of 15
No
Yes
12 of 15
No
Yes
7 of 15
No
Yes
6 of 15
No
Yes
5 of 15
No
Yes
4 of 15
No
Yes
2 of 15
No
No
2 of 15
No
No
2 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Caenorhabditis elegans (Nematode, roundworm) (13)
15 of 15
Yes
Yes
7 of 15
No
Yes
2 of 15
No
Yes
2 of 15
No
No
2 of 15
No
Yes
2 of 15
No
Yes
1 of 15
No
No
1 of 15
No
Yes
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
1 of 15
No
No
Arabidopsis thaliana (thale-cress) (22)
4 of 9
Yes
No
3 of 9
No
No
3 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
2 of 9
No
No
1 of 9
No
Yes
1 of 9
No
Yes
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
1 of 9
No
No
Saccharomyces cerevisiae (Brewer's yeast) (7)
12 of 15
Yes
Yes
4 of 15
No
No
3 of 15
No
No
3 of 15
No
No
3 of 15
No
No
2 of 15
No
No
1 of 15
No
No
Schizosaccharomyces pombe (Fission yeast) (3)
10 of 12
Yes
Yes
2 of 12
No
No
2 of 12
No
No
Other Organism Orthologs (via OrthoDB)
Paralogs
Paralogs (via DIOPT v8.0)
Drosophila melanogaster (Fruit fly) (10)
9 of 10
5 of 10
4 of 10
4 of 10
2 of 10
2 of 10
2 of 10
2 of 10
2 of 10
1 of 10
Human Disease Associations
FlyBase Human Disease Model Reports
Disease Model Summary Ribbon
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Allele
Disease
Evidence
References
Potential Models Based on Orthology ( 0 )
Human Ortholog
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Allele
Disease
Interaction
References
Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)
Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.
Functional Complementation Data
Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.
Interactions
Summary of Physical Interactions
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Interactions Browser

Please see the Physical Interaction reports below for full details
protein-protein
Physical Interaction
Assay
References
Summary of Genetic Interactions
esyN Network Diagram
esyN Network Key:
Suppression
Enhancement

Please look at the allele data for full details of the genetic interactions
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
Starting gene(s)
Interaction type
Interacting gene(s)
Reference
External Data
Linkouts
BioGRID - A database of protein and genetic interactions.
DroID - A comprehensive database of gene and protein interactions.
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Pathways
Signaling Pathways (FlyBase)
Negative Regulators of Hippo Signaling Pathway -
The Hippo signaling pathway is an intracellular kinase cascade in which hpo kinase in complex with sav, phosphorylates wts kinase which, in turn, phosphorylates yki transcriptional co-activator leading to its cytosolic retention. Negative regulators of the pathway promote the nuclear accumulation of yki, enhancing yki-mediated transcriptional regulation and the expression of genes that positively regulate tissue growth (Adapted from FBrf0224870).
Metabolic Pathways
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
External Data
Genomic Location and Detailed Mapping Data
Chromosome (arm)
2L
Recombination map
2-49
Cytogenetic map
Sequence location
FlyBase Computed Cytological Location
Cytogenetic map
Evidence for location
34D1-34D1
Limits computationally determined from genome sequence between P{lacW}CG7364k07245 and P{lacW}Sosk05224&P{lacW}RpII33k05605
Experimentally Determined Cytological Location
Cytogenetic map
Notes
References
34C4-34D1
34D-34D
(determined by in situ hybridisation)
34C4-34D1
(determined by in situ hybridisation)
34C-34C
(determined by in situ hybridisation)
Experimentally Determined Recombination Data
Location
Left of (cM)
Right of (cM)
Notes
Stocks and Reagents
Stocks (13)
Genomic Clones (23)
cDNA Clones (164)
 

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced
BDGP DGC clones
Other clones
Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

cDNA Clones, End Sequenced (ESTs)
Other clones
RNAi and Array Information
Linkouts
DRSC - Results frm RNAi screens
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
Antibody Information
Laboratory Generated Antibodies
 
Commercially Available Antibodies
 
Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
Other Information
Relationship to Other Genes
Source for database identify of

Source for identity of: p38b CG7393

Source for database merge of
Additional comments

The p38a and p38b genes share at least 50% identity.

Other Comments

p38b is expressed in clock neurons, and mutants in p38b either are arrhythmic or have a longer free-running periodicity, especially as they age.

p38b interacts with per to regulate period length and the strength of rhythmicity.

p38b activation in response to NaCl and heat shock is markedly reduced in Mekk1 mutants compared to wild type.

Mekk1 regulation of the Mpk2/p38b pathway is critical for the response to environmental stresses.

p38b functions in dpp signaling in wing morphogenesis.

Origin and Etymology
Discoverer
Etymology
Identification
External Crossreferences and Linkouts ( 82 )
Sequence Crossreferences
NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.
UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Other crossreferences
AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.
BDGP expression data - Patterns of gene expression in Drosophila embryogenesis
Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones
DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species
EMBL-EBI Single Cell Expression Atlas - Single cell expression across species
Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.
FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array
FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data
Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns
Flygut - An atlas of the Drosophila adult midgut
FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.
GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents
KEGG Genes - Molecular building blocks of life in the genomic space.
MARRVEL_MODEL - MARRVEL (model organism gene)
modMine - A data warehouse for the modENCODE project
SignaLink - A signaling pathway resource with multi-layered regulatory networks.
Linkouts
BioGRID - A database of protein and genetic interactions.
Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
Cell Signaling Technology - Commercial vendor for primary antibodies and antibody conjugates.
DroID - A comprehensive database of gene and protein interactions.
DRSC - Results frm RNAi screens
FlyCyc Genes - Genes from a BioCyc PGDB for Dmel
FlyMine - An integrated database for Drosophila genomics
Interactive Fly - A cyberspace guide to Drosophila development and metazoan evolution
MIST (genetic) - An integrated Molecular Interaction Database
MIST (protein-protein) - An integrated Molecular Interaction Database
Synonyms and Secondary IDs (31)
Reported As
Symbol Synonym
ESTS:186F5S
Mpk34C
anon-sts23
p38b
(Gera et al., 2022, Fabian et al., 2021, Layalle et al., 2021, Nishida et al., 2021, Scepanovic et al., 2021, Schneider and Imler, 2021, Xie et al., 2021, Li et al., 2020, Tafesh-Edwards and Eleftherianos, 2020, Wang et al., 2020, Hegazi et al., 2019, Li et al., 2019, Lim et al., 2019, Sun et al., 2019, Sun et al., 2019, Romey-Glüsing et al., 2018, Eom et al., 2017, Houtz et al., 2017, Kenmoku et al., 2017, Lin et al., 2017, Liu and Jin, 2017, Nandi et al., 2017, Pimmett et al., 2017, Terriente-Félix et al., 2017, Ashton-Beaucage et al., 2016, Clandinin and Owens, 2016-, Huang et al., 2016, Schimizzi et al., 2016, Luo et al., 2015, Pasco et al., 2015, Santabárbara-Ruiz et al., 2015, Ugrankar et al., 2015, Zhan et al., 2015, Belozerov et al., 2014, Chakrabarti et al., 2014, Lim et al., 2014, Sopko et al., 2014, White-Grindley et al., 2014, Wongtrakul et al., 2014, Carter, 2013, Freeman et al., 2013, Klinedinst et al., 2013, Schertel et al., 2013, Schoborg et al., 2013, Gomez et al., 2012, Gonda et al., 2012, Igboin et al., 2012, Vrailas-Mortimer et al., 2012, Wongtrakul et al., 2012, Mathew et al., 2011, Seisenbacher et al., 2011, Vrailas-Mortimer et al., 2011, Chen et al., 2010, Chen et al., 2010, Cully et al., 2010, Debat et al., 2009, Davis et al., 2008, Beltran et al., 2007, Langille and Clark, 2007, Liu et al., 2007, Sackton et al., 2007, Balakireva et al., 2006, Collins et al., 2006, Dworkin and Gibson, 2006, Dworkin and Gibson., 2006, Edwards et al., 2006, Sano et al., 2005, Cha et al., 2003)
Name Synonyms
p38 MAP kinase
p38 mitogen-activated protein kinase
p38b MAP kinase
stress-activated p38b MAP kinase
Secondary FlyBase IDs
  • FBgn0014421
  • FBgn0024951
  • FBgn0027921
Datasets (0)
Study focus (0)
Experimental Role
Project
Project Type
Title
Study result (0)
Result
Result Type
Title
References (192)