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Gene Dmel\CG2658

General Information
Symbol	Dmel\CG2658	Species	D. melanogaster
Name	CG2658	Annotation symbol	CG2658
Feature type	protein_coding_gene	FlyBase ID	FBgn0024992
Created / Updated	2003-12-01/2003-12-01
Genomic Location
Chromosome (arm)	X	Recombination map	1-
Cytogenetic map	3B2-3B2	Sequence location	X:2,588,755..2,592,248 [-]
Map ( GBrowse )	Decorated FastA GenBankGFF Gene region Extended Gene region CDSIntronsExonsTranslationsTranscripts3' UTR5' UTR
Summary Information
Automatically generated summary See sections below for more information	This gene is referred to in FlyBase by the symbol CG2658 (FBgn0024992). It has the cytological map location 3B2. Its sequence location is X:2588755..2592248. Its molecular function is described as: metallopeptidase activity; ATPase activity; metalloendopeptidase activity; ATP binding; zinc ion binding. It is involved in the biological processes: proteolysis; ATP-dependent proteolysis; protein catabolic process. 6 alleles are reported. No phenotypic data is available. It has 2 annotated transcripts and 2 annotated polypeptides.
Detailed Mapping Data
FlyBase Computed Cytological Location
Cytogenetic map Evidence for location 3B2-3B2   Limits computationally determined from genome sequence between P{EP}EP1362 and P{EP}dncEP1395
Experimentally Determined Cytological Location
Cytogenetic map Notes References
Experimentally Determined Recombination Data
Location	1- (Zehring et al., 1984)
Left of (cM)
Right of (cM)
Notes
Molecular Map Data
Gene Order (in direction of increasing cytology) References In direction of increasing cytology: per+ CG2650+ CG2658+ Csat- (Zehring et al., 1984, Reddy et al., 1984) Gene Order (overall orientation not stated) References Overall orientation not stated: per+ CG2650- CG2658- Csat- (Gatt and Ashburner, 2000.2.8)
Gene Model & Products
Please see the GBrowse view of Dmel\CG2658 for information on other features
Comments on Gene Model
Transcript Data
Annotated Transcripts
Name FlyBase ID RefSeq ID Length (nt) Associated CDS (aa) CG2658-RA FBtr0070502  NM_130661   3033   819 CG2658-RB FBtr0070503  NM_206616   3088   504
Additional Transcript Data & Comments
Reported size (kB)
Comments
External Data
Crossreferences
Polypeptide Data
Annotated Polypeptides
Name FlyBase ID Predicted MW (kD) Length (aa) Theoretical pI RefSeq ID GenBank protein CG2658-PA   FBpp0070479   90.1   819   8.76     NP_570017   AAF45806 CG2658-PB   FBpp0089098   55.4   504   9.97     NP_996339   AAS65257
Additional Polypeptide Data & Comments
Reported size (kD)
Comments
External Data
Linkouts	PANTHER - Protein classification by function, families, and pathways • FBgn0024992
Crossreferences	InterPro domains - A database of protein families, domains, and functional sites • Peptidase M41 (IPR000642) AAA+ ATPase, core (IPR003593) AAA ATPase, core (IPR003959) Peptidase M41, FtsH (IPR005936) Peptidase M41, FtsH extracellular (IPR011546) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME02658
Sequences Consistent with the Gene Model
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AE003425 AAF45806   AE003425 AAS65257   AI061982   AI514134   AI514429   AI542173   AL023874 CAA19646   AY118458 AAM49827   BF488907   BF492465   BF495697   BF495701   BF495703   BF498180   BF499002   BF499009   BF501772   BF503506   BF503513   BF504830   BF505846   BF505969   BG633867   BI213413   BI239572   BI357484   BI367174   BI636982   CK605166   CK605167   CO153616   CO178554   CO185672   CO279417   CO328770   EC055999   EC058313   EC068571   EC072759   EC243038   EC267307   UT01727
UniProtKB/Swiss-Prot
UniProtKB/TrEMBL	O76867 Q8MT03 Q9W4W8
Maps to
Does NOT map to
Identified with	GH01006 (BDGP Project Members, 2000-, Benos et al., 2001)
Mapped Features & Mutations
Please see GBrowse or insertion reports for information on insertions of transgenic constructs and features not listed here Type Symbol & Location Additional Notes References
External Data
Linkouts	DEDB - Drosophila exon database: splicing graphs • 364
Crossreferences
Expression Data
FlyBase-Curated Data
Transcript and Protein data	Please see the FlyBase Gene Expression Report for details of gene expression from the literature.
Summary of Transcript Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
Summary of Polypeptide Expression
Stage Tissue/Position Reference
Marker for
Subcellular Localization
CV Term
External Data & Images
Linkouts	FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024992 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0024992
Alleles & Phenotypes
Summary of Allele Phenotypes
Lethality Allele viable CG2658e03358 CG2658f06350 Sterility Allele fertile CG2658e03358 CG2658f06350 Phenotype manifest in Allele
Classical Alleles ( 2 )
For All Classical Alleles Show
Allele of CG2658 Class Mutagen Stocks Known lesion CG2658e03358 piggyBac transposase 2 -- CG2658f06350 piggyBac transposase 2 --
Alleles Carried on Transgenic Constructs ( 4 )
For All Alleles Carried on Transgenic Constructs Show
Allele of CG2658 Class Mutagen Stocks Known lesion CG265814.6 in vitro construct | genomic fragment 0 Yes CG26587.3 in vitro construct | genomic fragment 0 Yes CG26589.8 in vitro construct | genomic fragment 0 Yes CG2658GD14980 in vitro construct | RNAi in vitro construct | regulatory fusion 0 Yes
Aneuploid Aberrations
Useful deficiency	Df(1)w-rJ2
Useful duplication	Dp(1;2)w+70h
Transgenic Constructs & Insertions
Transgenic Constructs
	Type of construct Name Expression data UAS construct P{GD14980} NA
Insertions
	Type of insertions Name Expression data miscellaneous insertions PBac{RB}CG2658e03358 NA PBac{WH}CG2658f06350 NA
Related Comments
	Please look at the allele reports for the complete phenotype data
Gene Ontology: Function, Process & Cellular Component ( 10 )
Molecular Function
CV term Evidence References
ATP binding inferred from electronic annotation with InterPro:IPR000642, InterPro:IPR003959, InterPro:IPR011546 (FlyBase Curators et al., 2004-)
ATPase activity inferred from sequence or structural similarity with UniProtKB:Q9UQ90 (Sardiello et al., 2003.6.11)
metalloendopeptidase activity inferred from electronic annotation with InterPro:IPR000642, InterPro:IPR005936, InterPro:IPR011546 (FlyBase Curators et al., 2004-)
metallopeptidase activity inferred from sequence or structural similarity with EMBL:U09358 (FlyBase, 1992-) inferred from sequence or structural similarity with UniProtKB:Q9UQ90 (Sardiello et al., 2003.6.11)
zinc ion binding inferred from electronic annotation with InterPro:IPR011546 (FlyBase Curators et al., 2004-)
Biological Process
CV term Evidence References
ATP-dependent proteolysis inferred from sequence or structural similarity with UniProtKB:Q9UQ90 (Sardiello et al., 2003.6.11)
protein catabolic process inferred from electronic annotation with InterPro:IPR005936 (FlyBase Curators et al., 2004-)
proteolysis inferred from sequence or structural similarity with EMBL:U09358 (FlyBase, 1992-)
Cellular Component
CV term Evidence References
integral to membrane inferred from electronic annotation with InterPro:IPR011546 (FlyBase Curators et al., 2004-)
mitochondrion inferred from sequence or structural similarity with EMBL:U09358 (FlyBase, 1992-) inferred from sequence or structural similarity with UniProtKB:Q9UQ90 (Sardiello et al., 2003.6.11)
Sequence Ontology: Class of Gene
	protein_coding_gene   nuclear_gene
Interactions & Pathways
Summary of Genetic Interactions
Interacts with	Please look at the allele data for full details of the genetic interactions CG2658 allele Gene References
External Data
Linkouts	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 57784
Orthologs
Genome-wide drosophilid orthologs	Dana\GF21370 Dere\GG18614 Dgri\GH12676 Dmoj\GI16253 Dper\GL18240 Dpse\GA15413 Dsec\GM18863 Dsim\GD24604 Dvir\GJ16718 Dwil\GK19973 Dyak\GE16929
Curated drosophilid orthologs	Dpse\GA15413
Linkouts	InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000017298 Anopheles gambiae (Ensembl) ENSBTAP00000032431 Bos taurus (Ensembl) ENSCAFP00000029382 Canis familiaris (Ensembl) ENSCINP00000005638 Ciona intestinalis (Ensembl) NEWSINFRUP00000132661 Takifugu rubripes (Ensembl) ENSGALP00000010002 Gallus gallus (Ensembl) ENSP00000268704 Homo sapiens (Ensembl) ENSMMUP00000023558 Macaca mulatta (Ensembl) ENSMODP00000004549 Monodelphis domestica (Ensembl) ENSRNOP00000020548 Rattus norvegicus (Ensembl) GSTENP00017874001 Tetraodon nigroviridis (Ensembl) ENSXETP00000042933 Xenopus tropicalis (Ensembl) MGI:2385906 Mus musculus (Informatics)
Functional Complementation between Species
Inter-Species Misexpression Data
Produces phenotype in
Produces NO phenotype in
Stocks & Reagents
Stocks Listed in FlyBase ( 4 )
Harvard	- PBac{RB}CG2658e03358 - PBac{WH}CG2658f06350
Bloomington	18149 PBac{RB}CG2658e03358 w1118 18967 PBac{WH}CG2658f06350 w1118
Genomic Clones ( 1 )
	BACR30B01
cDNA Clones ( 31 )
	Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.
cDNA Clones, Fully Sequenced
BDGP DGC clones	GH01006
Other clones
cDNA Clones, End Sequenced (ESTs)
BDGP DGC clones	AT12671 LD40934 AT01544 AT28717 SD18849 SD08307 AT29821 RE36107 AT03251 AT08310 AT16214 GH27203 AT17319 RE52882 AT12554 AT04180 AT06861 RE18810 AT08075 AT13873 AT20769 RE43987 AT11146
Other clones	EK291401 EN02442 EK010826 EK134257 UT01727 EK057027 EC055999
RNAi & Array Information
Affy Oligo
Linkouts	DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0024992 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0024992
Antibody Information
Other Information
Discoverer
Etymology
Identification
Position Effect Variegation Data
Relationship to Other Genes
Source for database identity of	Source for identity of: EG:100G10.7 CG2658 (Deng, 1999.11)
Source for database merge of	Source for merge of: EG:100G10.7 anon-3Ba (Gatt and Ashburner, 2000.2.8)
Additional comments
Comments About Role
	Encodes a 2.7kb transcript flanking the 3' end of the 0.9kb transcript of CG2650, and transcribed in the same direction as CG2650. (Zehring et al., 1984)
Comments About Molecular Function
	The direction of transcription reported in FBrf0040494 is in error: CG2658 is transcribed off the opposite strand to per. (Gatt and Ashburner, 2000.2.8)
Other Comments
	"Gene order: Overall orientation not stated: per+ CG2650- CG2658- Csat-" was stated as revision. (Gatt and Ashburner, 2000.2.8)
External Crossreferences & Linkouts
Sequence Crossreferences
	RefSeq (Transcripts) • NM_130661 NM_206616 RefSeq (Proteins) • NP_570017 NP_996339 DDBJ / EMBL / GenBank • AE003425 AAF45806 AAS65257 AI061982 AL023874 CAA19646 AY118458 AAM49827 UniProtKB/TrEMBL • O76867 Q8MT03 Q9W4W8
Other Crossreferences
	InterPro domains - A database of protein families, domains, and functional sites • Peptidase M41 (IPR000642) AAA+ ATPase, core (IPR003593) AAA ATPase, core (IPR003959) Peptidase M41, FtsH (IPR005936) Peptidase M41, FtsH extracellular (IPR011546) MitoDrome - A database of annotated Dmel nuclear genes encoding mitochondrial proteins • MTDROME02658
Linkouts
	BioGRID - Interaction data, including yeast 2-hybrid and genetic interactions • 57784 DEDB - Drosophila exon database: splicing graphs • 364 DRSC - RNAi screening (Harvard): high-throughput cell culture data and design • FBgn0024992 FLIGHT - Cell culture data for RNAi and other high-throughput technologies • FBgn0024992 FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans • FBgn0024992 GEO (NCBI) - Gene expression data: microarray and other high-throughput technologies • FBgn0024992 GenomeRNAi - RNAi phenotypes (Heidelberg): high-throughput cell culture data • FBgn0024992 InParanoid orthologs - Eukaryotic orthologs • ENSANGP00000017298 Anopheles gambiae (Ensembl) ENSBTAP00000032431 Bos taurus (Ensembl) ENSCAFP00000029382 Canis familiaris (Ensembl) ENSCINP00000005638 Ciona intestinalis (Ensembl) NEWSINFRUP00000132661 Takifugu rubripes (Ensembl) ENSGALP00000010002 Gallus gallus (Ensembl) ENSP00000268704 Homo sapiens (Ensembl) ENSMMUP00000023558 Macaca mulatta (Ensembl) ENSMODP00000004549 Monodelphis domestica (Ensembl) ENSRNOP00000020548 Rattus norvegicus (Ensembl) GSTENP00017874001 Tetraodon nigroviridis (Ensembl) ENSXETP00000042933 Xenopus tropicalis (Ensembl) MGI:2385906 Mus musculus (Informatics) PANTHER - Protein classification by function, families, and pathways • FBgn0024992
Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	anon-3Ba (Benos et al., 2001, Reddy et al., 1984, ) CG2658   Dm100G10.aj (Frohlich, 2001) EG:100G10.7 (Gatt and Ashburner, 2000.2.8, Giot et al., 2003, ) unnamed (Dushay et al., 1990, Zehring et al., 1984)
Name Synonym	CG2658   paraplegin_DROME (Kammermeier et al., 2003)
Secondary FlyBase IDs
	FBgn0015870
References ( 20 )
Research paper	Kammermeier et al., 2003, Dev. Genes Evol. 213(8): 412--415 Identification of the Drosophila melanogaster homolog of the human spastin gene. [FBrf0162065] Pile et al., 2003, J. Biol. Chem. 278(39): 37840--37848 The SIN3 deacetylase complex represses genes encoding mitochondrial proteins: Implications for the regulation of energy metabolism. [FBrf0162185] Benos et al., 2001, Genome Res. 11(5): 710--730 From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823] Dushay et al., 1990, Genetics 125: 557--578 Phenotypic and genetic analysis of Clock, a new circadian rhythm mutant in Drosophila melanogaster. [FBrf0051955] Reddy et al., 1984, Cell 38: 701--710 Molecular analysis of the period locus in Drosophila melanogaster and identification of a transcript involved in biological rhythms. [FBrf0040494] Zehring et al., 1984, Cell 39: 369--376 P-element transformation with period locus DNA restores rhythmicity to mutant, arrhythmic Drosophila melanogaster. [FBrf0040481]
Supplementary material	Dietzl et al., 2007, Nature 448(7150): Supplementary Table 1. [FBrf0202579] Giot et al., 2003, Science 302(5651): Tables S1 to S7. [FBrf0166452]
Review	Frohlich, 2001, J. Cell Sci. 114(9): 1601--1602 An AAA family tree. [FBrf0136000] Juhola et al., 2000, FEBS Lett. 481(2): 91--95 The mitochondrial inner membrane AAA metalloprotease family in metazoans. [FBrf0129878]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2004.11.6, Exelixis insertion alleles that are not in FlyBase. Exelixis insertion alleles that are not in FlyBase. [FBrf0179797] FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215] Sardiello et al., 2003.6.11, MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion. MitoDrome: A database of Drosophila melanogaster nuclear genes encoding proteins targeted to the mitochondrion. [FBrf0159903] Benos, 2000.12.13, FlyBase error report on Wed Dec 13 14:35:56 2000. FlyBase error report on Wed Dec 13 14:35:56 2000. [FBrf0133222] Gatt and Ashburner, 2000.2.8, EG:100G10.7 and EG:100G10.5. EG:100G10.7 and EG:100G10.5. [FBrf0125091]
FlyBase analysis	FlyBase, 1992-, FlyBase curation FlyBase curation. [FBrf0105495]
Curated genome annotation	Ashburner, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126651] Deng, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0128757]
Computer file	BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. Berkeley Drosophila Genome Project. [FBrf0125078]
Automatic genome annotation	FamiliarityBreedsContempt, 1999.11, [Annotations performed at the Celera Jamboreee.] [Annotations performed at the Celera Jamboreee.] [FBrf0126705]