Gene Dmel\CG2685

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General Information

Symbol

Dmel\CG2685

Species

D. melanogaster

Name

Annotation symbol

CG2685

Feature type

protein_coding_gene

FlyBase ID

FBgn0024998

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

EG:100G10.1

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

3B3-3B3

Sequence location

X:2,605,789..2,607,993 [-]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

This gene is referred to in FlyBase by the symbol Dmel\CG2685 (FBgn0024998). It is a protein_coding_gene from Drosophila melanogaster. Its molecular function is unknown. It is reported to be involved in the biological process: mRNA splicing, via spliceosome. 3 alleles are reported. The phenotype of these alleles is annotated with: mesothoracic tergum. It has one annotated transcript and one annotated polypeptide. Protein features are: WW domain binding protein 11. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary. Comments on Affy2 ProbeSet: ProbeSet 1629864_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of CG2685. Gene sequence location is X:2605789..2607993.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

3B3-3B3

Limits computationally determined from genome sequence between P{EP}EP1362 and P{EP}dnc^EP1395

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\CG2685 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Gene model reviewed during 5.43

Gene model reviewed during 5.50

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

CG2685-RA

FBtr0070499

NM_130667

1952

552

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

CG2685-PA

FBpp0070476

61.0

552

7.62

NP_570023

AAF45812

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WW domain binding protein 11 (IPR019007)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

catalytic step 2 spliceosome

inferred from direct assay

(Herold et al., 2009)

precatalytic spliceosome

inferred from direct assay

(Herold et al., 2009)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult ovary.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		83.75
Larval Midgut		28
Larval Hindgut		32.3
Larval Malpighian Tubules		48.1
Larval Fat Body		32.3
Larval Salivary Gland		59.7
Larval Trachea		32.25
Larval Carcass		38.75
Adult Head		35.2
Adult Eye		36.275
Adult Brain		74
Adult Thoracic-Abdominal Ganglion		73.1
Adult Crop		51.5
Adult Midgut		23.9
Adult Hindgut		33.5
Adult Malpighian Tubules		33.3
Adult Fat Body		29
Adult Salivary Gland		69.9
Adult Heart		20.95
Adult VirginFemale Spermatheca		36.9
Adult InseminatedFemale Spermatheca		39.3
Adult Ovary		125.3
Adult Testis		28.9
Adult Male Accessory Gland		68
Adult Carcass		41.2
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	35.2
NA	Eye	36.275
NA	Brain	74
83.75	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	73.1
NA	Crop	51.5
28	Midgut	23.9
32.3	Hindgut	33.5
48.1	Malpighian Tubules	33.3
32.3	Fat Body	29
59.7	Salivary Gland	69.9
NA	Heart	20.95
32.25	Trachea	NA
NA	VirginFemale Spermatheca	36.9
NA	InseminatedFemale Spermatheca	39.3
NA	Ovary	125.3
NA	Testis	28.9
NA	Male Accessory Gland	68
38.75	Carcass	41.2

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		40
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		48
ovary, mated 4-day female		100
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		(40)
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		(29)
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		(48)
ovary, mated 4-day female		(100)
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		40
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		48
ovary, mated 4-day female		100
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		40
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		48
ovary, mated 4-day female		100
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		40
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		48
ovary, mated 4-day female		100
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		(40)
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		(48)
ovary, mated 4-day female		(100)
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		40
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		48
ovary, mated 4-day female		100
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		20
central nervous system, larvae L3		40
central nervous system, pupae P8		19
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		7
head, mated 1-day female		22
head, mated 4-day female		16
head, mated 20-day female		8
head, mated 1-day male		18
head, mated 4-day male		20
head, mated 20-day male		24
salivary gland, larvae L3 wandering		3
salivary gland, white prepupae		4
digestive system, larvae L3 wandering		15
digestive system, 1-day adult		9
digestive system, 4-day adult		15
digestive system, 20-day adult		9
fat body, larvae L3 wandering		7
fat body, white prepupae		15
fat body, pupae P8		29
carcass, larvae L3 wandering		24
carcass, 1-day adult		9
carcass, 4-day adult		13
carcass, 20-day adult		11
ovary, virgin 4-day female		48
ovary, mated 4-day female		100
testis, mated 4-day male		13
accessory gland, mated 4-day male		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of moderately high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages, in adult female stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		44
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(46)
embryo 02-04hr		(44)
embryo 04-06hr		(30)
embryo 06-08hr		(31)
embryo 08-10hr		(28)
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		44
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		44
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		44
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(46)
embryo 02-04hr		(44)
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		44
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		46
embryo 02-04hr		44
embryo 04-06hr		30
embryo 06-08hr		31
embryo 08-10hr		28
embryo 10-12hr		22
embryo 12-14hr		15
embryo 14-16hr		15
embryo 16-18hr		11
embryo 18-20hr		9
embryo 20-22hr		8
embryo 22-24hr		9
larva L1		10
larva L2		8
larva L3 12hr old		7
larva L3 puffstage 1-2		7
larva L3 puffstage 3-6		9
larva L3 puffstage 7-9		9
white prepupae new		10
white prepupae 12hr		12
white prepupae 24hr		14
pupae 2d postWPP		9
pupae 3d postWPP		6
pupae 4d postWPP		5
adult male 01day		6
adult male 05day		8
adult male 30day		9
adult female 01day		13
adult female 05day		19
adult female 30day		21
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		35
Schneider line 2 Sg4		28
embryonic 1182-4H		40
embryonic GM2		17
embryonic Kc167		31
embryonic S1		46
embryonic S3		34
leg disc CME L1		42
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		49
wing disc ML-DmD16-c3		50
wing disc ML-DmD21		55
wing disc ML-DmD32		39
haltere disc ML-DmD17-c3		36
eye-antennal disc ML-DmD11		37
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		58
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		103
ovary OSC		77
ovary OSS		46
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(35)
Schneider line 2 Sg4		(28)
embryonic 1182-4H		(40)
embryonic GM2		17
embryonic Kc167		(31)
embryonic S1		(46)
embryonic S3		(34)
leg disc CME L1		(42)
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		(49)
wing disc ML-DmD16-c3		(50)
wing disc ML-DmD21		(55)
wing disc ML-DmD32		(39)
haltere disc ML-DmD17-c3		(36)
eye-antennal disc ML-DmD11		(37)
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		(34)
CNS ML-DmBG1-c1		(58)
CNS ML-DmBG2-c2		(29)
tumorous blood cells mbn2		16
ovary fGS/OSS		(103)
ovary OSC		(77)
ovary OSS		(46)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		35
Schneider line 2 Sg4		28
embryonic 1182-4H		40
embryonic GM2		17
embryonic Kc167		31
embryonic S1		46
embryonic S3		34
leg disc CME L1		42
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		49
wing disc ML-DmD16-c3		50
wing disc ML-DmD21		55
wing disc ML-DmD32		39
haltere disc ML-DmD17-c3		36
eye-antennal disc ML-DmD11		37
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		58
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		103
ovary OSC		77
ovary OSS		46
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		35
Schneider line 2 Sg4		28
embryonic 1182-4H		40
embryonic GM2		17
embryonic Kc167		31
embryonic S1		46
embryonic S3		34
leg disc CME L1		42
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		49
wing disc ML-DmD16-c3		50
wing disc ML-DmD21		55
wing disc ML-DmD32		39
haltere disc ML-DmD17-c3		36
eye-antennal disc ML-DmD11		37
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		58
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		103
ovary OSC		77
ovary OSS		46
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		35
Schneider line 2 Sg4		28
embryonic 1182-4H		40
embryonic GM2		17
embryonic Kc167		31
embryonic S1		46
embryonic S3		34
leg disc CME L1		42
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		49
wing disc ML-DmD16-c3		50
wing disc ML-DmD21		55
wing disc ML-DmD32		39
haltere disc ML-DmD17-c3		36
eye-antennal disc ML-DmD11		37
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		58
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		103
ovary OSC		77
ovary OSS		46
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		(35)
Schneider line 2 Sg4		28
embryonic 1182-4H		(40)
embryonic GM2		17
embryonic Kc167		31
embryonic S1		(46)
embryonic S3		(34)
leg disc CME L1		(42)
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		(49)
wing disc ML-DmD16-c3		(50)
wing disc ML-DmD21		(55)
wing disc ML-DmD32		(39)
haltere disc ML-DmD17-c3		(36)
eye-antennal disc ML-DmD11		(37)
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		(34)
CNS ML-DmBG1-c1		(58)
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		(103)
ovary OSC		(77)
ovary OSS		(46)
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		35
Schneider line 2 Sg4		28
embryonic 1182-4H		40
embryonic GM2		17
embryonic Kc167		31
embryonic S1		46
embryonic S3		34
leg disc CME L1		42
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		49
wing disc ML-DmD16-c3		50
wing disc ML-DmD21		55
wing disc ML-DmD32		39
haltere disc ML-DmD17-c3		36
eye-antennal disc ML-DmD11		37
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		58
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		103
ovary OSC		77
ovary OSS		46
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		35
Schneider line 2 Sg4		28
embryonic 1182-4H		40
embryonic GM2		17
embryonic Kc167		31
embryonic S1		46
embryonic S3		34
leg disc CME L1		42
wing disc CME-W2		19
wing disc ML-DmD8		18
wing disc ML-DmD9		49
wing disc ML-DmD16-c3		50
wing disc ML-DmD21		55
wing disc ML-DmD32		39
haltere disc ML-DmD17-c3		36
eye-antennal disc ML-DmD11		37
antennal disc ML-DmD20-c5		19
mixed discs ML-DmD4-c1		34
CNS ML-DmBG1-c1		58
CNS ML-DmBG2-c2		29
tumorous blood cells mbn2		16
ovary fGS/OSS		103
ovary OSC		77
ovary OSS		46
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		64
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		57
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		39
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		55
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(45)
cold shock, 4-day adult		(31)
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		(30)
Cadmium 100 mM 48 hrs, 4-day adult		(64)
Copper 0.5 mM 12 hrs, larvae L3		(29)
Copper 15 mM 48 hrs, 4-day adult		(57)
Zinc 5 mM 12 hrs, larvae L3		(27)
Zinc 4.5 mM 48 hrs, 4-day adult		(27)
Ethanol 2.5% 3 hrs, larvae L3		(39)
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(57)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(40)
Paraquat 5 mM 48 hrs, 4-day adult		(55)
Paraquat 10 mM 48 hrs, 4-day adult		(31)
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		64
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		57
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		39
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		55
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		64
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		57
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		39
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		55
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		64
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		57
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		39
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		55
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		(45)
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		(64)
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		(57)
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		(39)
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(57)
Caffeine 25 mg/ml 48 hrs, 4-day adult		(40)
Paraquat 5 mM 48 hrs, 4-day adult		(55)
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		64
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		57
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		39
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		55
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		45
cold shock, 4-day adult		31
heat shock, 4-day adult		21
Cadmium 50 mM 6 hrs, larvae L3		11
Cadmium 50 mM 12 hrs, larvae L3		6
Cadmium 50 mM 48 hrs, 4-day adult		30
Cadmium 100 mM 48 hrs, 4-day adult		64
Copper 0.5 mM 12 hrs, larvae L3		29
Copper 15 mM 48 hrs, 4-day adult		57
Zinc 5 mM 12 hrs, larvae L3		27
Zinc 4.5 mM 48 hrs, 4-day adult		27
Ethanol 2.5% 3 hrs, larvae L3		39
Ethanol 5% 3 hrs, larvae L3		9
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		57
Caffeine 25 mg/ml 48 hrs, 4-day adult		40
Paraquat 5 mM 48 hrs, 4-day adult		55
Paraquat 10 mM 48 hrs, 4-day adult		31
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		6
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010, Graveley et al., 2011)

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0024998

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG2685-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

partially lethal - majority die | pupal stage, with Scer\GAL4^pnr-MD237

CG2685^GD9807

Other Phenotypes

Allele

visible, with Scer\GAL4^pnr-MD237

CG2685^GD9807

Phenotype manifest in

Allele

mesothoracic tergum, with Scer\GAL4^pnr-MD237

CG2685^GD9807

Classical Alleles ( 0 )

For All Classical Alleles Show

Allele of CG2685	Class	Mutagen	Stocks	Known lesion

Alleles Carried on Transgenic Constructs ( 3 )

For All Alleles Carried on Transgenic Constructs Show

Allele of CG2685	Mutagen	Stocks	Known lesion
CG2685^GD9807	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG2685^KK100421	in vitro construct - RNAi in vitro construct - regulatory fusion	1	Yes
CG2685^NIG.2685R	in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD9807}

P{KK100421}

P{NIG.2685R}

Insertions

Type of insertions

Name

Expression data

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

Terms Based on Experimental Evidence ( 2 terms )

Molecular Function ( 0 terms)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

catalytic step 2 spliceosome

inferred from direct assay

(Herold et al., 2009)

precatalytic spliceosome

inferred from direct assay

(Herold et al., 2009)

Terms Based on Predictions or Assertions ( 2 terms )

Molecular Function ( 0 terms)

Biological Process

CV term

Evidence

References

mRNA splicing, via spliceosome

inferred by curator from GO:0071011|GO:0071013

(Herold et al., 2009)

RNA processing

inferred from electronic annotation with InterPro:IPR019007

(FlyBase Curators et al., 2004-)

Cellular Component ( 0 terms)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

protein-protein

Interacting group

Assay

References

CG2685 - sbr

anti tag coimmunoprecipitation, anti tag western blot

(Farny et al., 2008)

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

CG2685 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

57790

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0024998

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31259

Orthologs

OrthoDB Orthologs (29) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG60P4SX

Dipteran inclusive ortholog search

EOG6F1XC8

Insect inclusive ortholog search

EOG6S4NBZ

Arthropod inclusive ortholog search

EOG6SQVCF

Metazoa inclusive ortholog search

No orthologs identified

Orthologs in Drosophila Species (EOG60P4SX)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15430

Drosophila persimilis

Dper\GL19810

Drosophila willistoni

Dwil\GK16223

Drosophila virilis

Dvir\GJ18536

Drosophila mojavensis

Dmoj\GI14730

Drosophila grimshawi

Dgri\GH11943

Orthologs in non-Drosophila Dipterans (EOG6F1XC8)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL001971

Anopheles gambiae

Malaria mosquito

Agam\AGAP013760

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ801595

Orthologs in non-Dipteran Insects (EOG6S4NBZ)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB42447

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG024982

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH21596

Atta cephalotes

Leafcutter ant

Acep\ACEP16202

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO18665

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL22275

Linepithema humile

Argentine ant

Lhum\LH19358

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB25037

Solenopsis invicta

Red fire ant

Sinv\SINV25505

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI003537

Bombyx mori

Silkmoth

Bmor\BGIBMGA012034

Pediculus humanus

Human body louse

Phum\PHUM414900

Tribolium castaneum

Red flour beetle

Tcas\TC000057

Orthologs in non-Insect Arthropods (EOG6SQVCF)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\cytb

Orthologs in non-Arthropod Metazoa (None identified)

No non-Arthropod Metazoa orthologies identified

Human Orthologs (0)

Gene

OMIM

HGNC

AAA Orthologs (10) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA15430

Drosophila persimilis

Dper\GL19810

Drosophila willistoni

Dwil\GK16223

Drosophila virilis

Dvir\GJ18536

Drosophila grimshawi

Dgri\GH11943

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

VDRC

v106918

P{KK100421}VIE-260B

v20887

w¹¹¹⁸; P{GD9807}v20887

Genomic Clones ( 1 )

BACR30B01

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 28 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones

Other clones

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi & Array Information

Linkouts

DRSC - Results from RNAi screens.

•

FBgn0024998

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31259

Antibody Information

Other Information

Discoverer

Etymology

Identification

Relationship to Other Genes

Source for database identity of

Source for identity of: EG:100G10.1 CG2685

(Deng, 1999.11)

Source for database merge of

Additional comments

Other Comments

External Crossreferences & Linkouts

Sequence Crossreferences

RefSeq (Transcripts)

•

NM_130667

RefSeq (Proteins)

•

NP_570023

DDBJ / EMBL / GenBank

•

Entrez Gene - A searchable database of RefSeq genes.

•

31259

UniProtKB/TrEMBL

•

Other Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

WW domain binding protein 11 (IPR019007)

Linkouts

BioGRID - A database of protein and genetic interactions

•

57790

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0024998

DRSC - Results from RNAi screens.

•

FBgn0024998

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0024998

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG2685-RA

FlyMine - Integrated genomics database for Drosophila, Anopheles, and C.elegans

•

FBgn0024998

GenomeRNAi - GenomeRNAi – A database for cell-based and in vivo RNAi phenotypes and reagents

•

31259

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

31259

modMine - Data generated by the modENCODE project.

•

FBgn0024998

Synonyms & Secondary IDs ( 2 )

Reported As

Symbol Synonym

CG2685

(Farny et al., 2008, Keleman et al., 2009.8.5, Herold et al., 2009, Japanese National Institute of Genetics, 2012.5.21)

EG:100G10.1

(Benos et al., 2001, Giot et al., 2003, )

Name Synonym

Secondary FlyBase IDs

References ( 15 )

Research paper

Herold et al., 2009, Mol. Cell. Biol. 29(1): 281--301: Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes. [FBrf0207172]
Farny et al., 2008, Genes Dev. 22(1): 66--78: Definition of global and transcript-specific mRNA export pathways in metazoans. [FBrf0200863]
Benos et al., 2001, Genome Res. 11(5): 710--730: From First Base: the sequence of the tip of the X chromosome of Drosophila melanogaster, a comparison of two sequencing strategies. [FBrf0135823]

Supplementary material

Graveley et al., 2011, Nature 471(7339):: Supplementary Table 33. Fuzzy Cluster Members. [FBrf0213980]
The modENCODE Consortium, 2010, Science 330(6012):: DataS14: Gene co-expression clusters. [FBrf0213979]
Mummery-Widmer et al., 2009, Nature 458(7241):: Supplementary Table 2 - Genome-wide bristle screen results. [FBrf0214518]
Giot et al., 2003, Science 302(5651):: Tables S1 to S7. [FBrf0166452]

Personal communication to FlyBase

Keleman et al., 2009.8.5, RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi-phiC31 construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0208510]
Dickson et al., 2007.7.18, RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center: RNAi construct and insertion data submitted by the Vienna Drosophila RNAi Center [FBrf0200327]
FlyBase Curators et al., 2004-, Gene Ontology annotation in FlyBase through association of InterPro records with GO terms.: Gene Ontology annotation in FlyBase through association of InterPro records with GO terms. [FBrf0174215]
BDGP Project Members, 2000-, Berkeley Drosophila Genome Project. (Computer file): Berkeley Drosophila Genome Project. (Computer file) [FBrf0125078]
Benos, 2000.12.13, FlyBase error report on Wed Dec 13 14:35:56 2000.: FlyBase error report on Wed Dec 13 14:35:56 2000. [FBrf0133222]

Stock list

Japanese National Institute of Genetics, 2012.5.21, RNAi stocks available from NIG-FLY.: RNAi stocks available from NIG-FLY. [FBrf0218457]

Curated genome annotation

Deng, 1999.11, [Annotations performed at the Celera Jamboreee.]: [Annotations performed at the Celera Jamboreee.] [FBrf0128757]