Gene Dmel\ari-2

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General Information

Symbol

Dmel\ari-2

Species

D. melanogaster

Name

ariadne 2

Annotation symbol

CG5709

Feature type

protein_coding_gene

FlyBase ID

FBgn0025186

Gene Model Status

Current

Stock availability

2 publicly available

Also Known As

l(2)07768, ari2

Genomic Location

Chromosome (arm)

Recombination map

Cytogenetic map

58D1-58D2

Sequence location

2R:18,100,340..18,104,769 [+]

Genomic Maps

Select View:

Summary Information

Automatically generated summary

See sections below for more information

The gene ariadne 2 is referred to in FlyBase by the symbol Dmel\ari-2 (CG5709, FBgn0025186). It is a protein_coding_gene from Drosophila melanogaster. It is reported to have molecular function: ubiquitin-protein ligase activity. The biological processes in which it is involved are not known. 16 alleles are reported. No phenotypic data is available. It has one annotated transcript and one annotated polypeptide. Protein features are: Zinc finger, C6HC-type; Zinc finger, CCHC-type; Zinc finger, RING-type; Zinc finger, RING-type, conserved site; Zinc finger, RING/FYVE/PHD-type. Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages. Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval hindgut, larval/adult Malpighian tubules, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass. Comments on Affy2 ProbeSet: ProbeSet 1625556_at completely aligns to an exonic region of the only FlyBase-annotated transcript isoform of ari-2. Gene sequence location is 2R:18100346..18104769.

User Contributed Data

Beta version, feedback welcome

Recent Updates

Description

What does this section display?

This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).

What does this section not display?

This section does not currently display links that were removed or gene model changes.

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FB2013_03

Alleles

ari-2[Or]

FB2013_02

All updates

Click here to see a list of all updates to this record from FB2010_08 and on.

Detailed Mapping Data

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

58D1-58D2

Limits computationally determined from genome sequence between P{EP}CG6393^EP2457 and P{PZ}ari-2⁰⁷⁷⁶⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

58C-58D

(Aguilera, 1998.8.18)

58D1-58D2

(determined by in situ hybridisation)

(Spradling et al., 1999)

58D1-58D2

(BDGP Project Members, 1994-1999)

Experimentally Determined Recombination Data

Location

Left of (cM)

Right of (cM)

Notes

Gene Model & Products

Please see the GBrowse view of Dmel\ari-2 for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.51

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Associated CDS (aa)

ari-2-RA

FBtr0071774

NM_058026

3008

509

Additional Transcript Data & Comments

Reported size (kB)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank protein

ari-2-PA

FBpp0071688

58.6

509

5.38

NP_477374

AAF46823

Additional Polypeptide Data & Comments

Reported size (kDa)

Comments

External Data

Linkouts

Crossreferences

InterPro domains - A database of protein families, domains, and functional sites

•

Zinc finger, RING-type (IPR001841)

Zinc finger, C6HC-type (IPR002867)

Zinc finger, CCHC-type (IPR001878)

Zinc finger, RING-type, conserved site (IPR017907)

Zinc finger, RING/FYVE/PHD-type (IPR013083)

Sequences Consistent with the Gene Model

DDBJ /
EMBL /
GenBank

DNA sequence

Protein sequence

Name

O76924

UniProtKB/TrEMBL

Q8MRP8

Mapped Features

Mapped Features have been reorganized, please see this article for details.

Additional mapped features and mutations can be found on GBrowse or related reports.

Type

Symbol & Location

Additional Notes

References

External Data

Linkouts

Crossreferences

Expression Data

Transcript Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization (GO Cellular Component)

CV term

Evidence

References

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

Reference

See Gelbart and Emmert, 2010.10.13 for analysis details and data files for all genes.

FlyAtlas Anatomy Microarray

FlyAtlas Anatomical Expression Data

(FlyAtlas-RNA.adult)

(FlyAtlas-RNA.larva)

	Styles
	Linear
	Logarithmic
	Heatmap
	Back-to-back
	Scales
	gene max expression
	Moderate expression bin max
	High level expression bin max
	Very high expression bin max

Summary of FlyAtlas Anatomical Expression Data: Expression at moderate levels in the following post-embryonic organs or tissues: adult head, adult eye, larval/adult central nervous system, adult crop, larval midgut, larval hindgut, larval/adult Malpighian tubules, larval fat body, larval/adult salivary gland, larval trachea, adult female reproductive system, adult male accessory gland, larval carcass.
[download data (TSV)]

Guide to FlyAtlas expression level colors
	No expression (0 - 9.999)
	Low expression (10 - 99.999)
	Moderate expression (100 - 499.999)
	High level expression (500 - 999.999)
	Very high expression (>999.999)

Linear, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate

Linear, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate High

Linear, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate High Very high

Linear, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		Very high

log, scaled to maximum expression level
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate High

log, scaled to Moderate expression
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate High

log, scaled to High level expression
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate High Very high

log, scaled to Very high expression
Tissue		Expression Level
Larval Central Nervous System		365.825
Larval Midgut		125
Larval Hindgut		115.3
Larval Malpighian Tubules		295.3
Larval Fat Body		213.2
Larval Salivary Gland		201.7
Larval Trachea		144.55
Larval Carcass		148.15
Adult Head		149.4
Adult Eye		270.95
Adult Brain		213
Adult Thoracic-Abdominal Ganglion		210.7
Adult Crop		145.1
Adult Midgut		84.7
Adult Hindgut		96.8
Adult Malpighian Tubules		117.3
Adult Fat Body		56.5
Adult Salivary Gland		114.6
Adult Heart		81.4
Adult VirginFemale Spermatheca		136.7
Adult InseminatedFemale Spermatheca		116
Adult Ovary		445.5
Adult Testis		62.6
Adult Male Accessory Gland		105.4
Adult Carcass		94.3
Expression Level Scale		None Low Moderate High Very high

Heatmap
Tissue		Expression Level
Larval Central Nervous System
Larval Midgut
Larval Hindgut
Larval Malpighian Tubules
Larval Fat Body
Larval Salivary Gland
Larval Trachea
Larval Carcass
Adult Head
Adult Eye
Adult Brain
Adult Thoracic-Abdominal Ganglion
Adult Crop
Adult Midgut
Adult Hindgut
Adult Malpighian Tubules
Adult Fat Body
Adult Salivary Gland
Adult Heart
Adult VirginFemale Spermatheca
Adult InseminatedFemale Spermatheca
Adult Ovary
Adult Testis
Adult Male Accessory Gland
Adult Carcass

FlyAtlas Organ/Tissue Expression, larval vs. adult
Larval Expression Level	Tissue	Adult Expression Level
NA	Head	149.4
NA	Eye	270.95
NA	Brain	213
365.825	Central Nervous System	NA
NA	Thoracic-Abdominal Ganglion	210.7
NA	Crop	145.1
125	Midgut	84.7
115.3	Hindgut	96.8
295.3	Malpighian Tubules	117.3
213.2	Fat Body	56.5
201.7	Salivary Gland	114.6
NA	Heart	81.4
144.55	Trachea	NA
NA	VirginFemale Spermatheca	136.7
NA	InseminatedFemale Spermatheca	116
NA	Ovary	445.5
NA	Testis	62.6
NA	Male Accessory Gland	105.4
148.15	Carcass	94.3

FlyAtlas Anatomical Expression Data (Chintapalli et al., 2007)

modENCODE Anatomy RNA-Seq

modENCODE Tissue Expression Data

(modENCODE_mRNA-Seq_tissues)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		50
central nervous system, pupae P8		36
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		46
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		33
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		60
ovary, mated 4-day female		57
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		(28)
central nervous system, larvae L3		(50)
central nervous system, pupae P8		(36)
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		(46)
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		(33)
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		(60)
ovary, mated 4-day female		(57)
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		50
central nervous system, pupae P8		36
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		46
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		33
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		60
ovary, mated 4-day female		57
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		50
central nervous system, pupae P8		36
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		46
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		33
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		60
ovary, mated 4-day female		57
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		50
central nervous system, pupae P8		36
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		46
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		33
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		60
ovary, mated 4-day female		57
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		(50)
central nervous system, pupae P8		(36)
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		(46)
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		(33)
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		(60)
ovary, mated 4-day female		(57)
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		50
central nervous system, pupae P8		36
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		46
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		33
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		60
ovary, mated 4-day female		57
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Tissue		Expression Level
imaginal disc, larvae L3 wandering		28
central nervous system, larvae L3		50
central nervous system, pupae P8		36
head, virgin 1-day female		16
head, virgin 4-day female		16
head, virgin 20-day female		10
head, mated 1-day female		16
head, mated 4-day female		10
head, mated 20-day female		11
head, mated 1-day male		23
head, mated 4-day male		18
head, mated 20-day male		17
salivary gland, larvae L3 wandering		12
salivary gland, white prepupae		46
digestive system, larvae L3 wandering		19
digestive system, 1-day adult		5
digestive system, 4-day adult		7
digestive system, 20-day adult		7
fat body, larvae L3 wandering		7
fat body, white prepupae		19
fat body, pupae P8		20
carcass, larvae L3 wandering		33
carcass, 1-day adult		11
carcass, 4-day adult		9
carcass, 20-day adult		10
ovary, virgin 4-day female		60
ovary, mated 4-day female		57
testis, mated 4-day male		10
accessory gland, mated 4-day male		5
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Tissue		Expression Level
imaginal disc, larvae L3 wandering
central nervous system, larvae L3
central nervous system, pupae P8
head, virgin 1-day female
head, virgin 4-day female
head, virgin 20-day female
head, mated 1-day female
head, mated 4-day female
head, mated 20-day female
head, mated 1-day male
head, mated 4-day male
head, mated 20-day male
salivary gland, larvae L3 wandering
salivary gland, white prepupae
digestive system, larvae L3 wandering
digestive system, 1-day adult
digestive system, 4-day adult
digestive system, 20-day adult
fat body, larvae L3 wandering
fat body, white prepupae
fat body, pupae P8
carcass, larvae L3 wandering
carcass, 1-day adult
carcass, 4-day adult
carcass, 20-day adult
ovary, virgin 4-day female
ovary, mated 4-day female
testis, mated 4-day male
accessory gland, mated 4-day male

modENCODE Development RNA-Seq

modENCODE Temporal Expression Data

(modENCODE_mRNA-Seq_U)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

Summary of modENCODE Temporal Expression Profile: Temporal profile ranges from a peak of high expression to a trough of low expression. Peak expression observed within 00-12 hour embryonic stages.
[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		53
embryo 02-04hr		36
embryo 04-06hr		29
embryo 06-08hr		50
embryo 08-10hr		55
embryo 10-12hr		57
embryo 12-14hr		45
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		39
white prepupae 24hr		35
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(53)
embryo 02-04hr		(36)
embryo 04-06hr		(29)
embryo 06-08hr		(50)
embryo 08-10hr		(55)
embryo 10-12hr		(57)
embryo 12-14hr		(45)
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		(28)
white prepupae new		(30)
white prepupae 12hr		(39)
white prepupae 24hr		(35)
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		(28)
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		53
embryo 02-04hr		36
embryo 04-06hr		29
embryo 06-08hr		50
embryo 08-10hr		55
embryo 10-12hr		57
embryo 12-14hr		45
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		39
white prepupae 24hr		35
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		53
embryo 02-04hr		36
embryo 04-06hr		29
embryo 06-08hr		50
embryo 08-10hr		55
embryo 10-12hr		57
embryo 12-14hr		45
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		39
white prepupae 24hr		35
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Developmental Stage		Expression Level
embryo 00-02hr		53
embryo 02-04hr		36
embryo 04-06hr		29
embryo 06-08hr		50
embryo 08-10hr		55
embryo 10-12hr		57
embryo 12-14hr		45
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		39
white prepupae 24hr		35
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Developmental Stage		Expression Level
embryo 00-02hr		(53)
embryo 02-04hr		(36)
embryo 04-06hr		29
embryo 06-08hr		(50)
embryo 08-10hr		(55)
embryo 10-12hr		(57)
embryo 12-14hr		(45)
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		(39)
white prepupae 24hr		(35)
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Developmental Stage		Expression Level
embryo 00-02hr		53
embryo 02-04hr		36
embryo 04-06hr		29
embryo 06-08hr		50
embryo 08-10hr		55
embryo 10-12hr		57
embryo 12-14hr		45
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		39
white prepupae 24hr		35
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Developmental Stage		Expression Level
embryo 00-02hr		53
embryo 02-04hr		36
embryo 04-06hr		29
embryo 06-08hr		50
embryo 08-10hr		55
embryo 10-12hr		57
embryo 12-14hr		45
embryo 14-16hr		24
embryo 16-18hr		19
embryo 18-20hr		24
embryo 20-22hr		10
embryo 22-24hr		10
larva L1		6
larva L2		3
larva L3 12hr old		4
larva L3 puffstage 1-2		9
larva L3 puffstage 3-6		18
larva L3 puffstage 7-9		28
white prepupae new		30
white prepupae 12hr		39
white prepupae 24hr		35
pupae 2d postWPP		22
pupae 3d postWPP		12
pupae 4d postWPP		13
adult male 01day		12
adult male 05day		13
adult male 30day		12
adult female 01day		15
adult female 05day		26
adult female 30day		28
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Developmental Stage		Expression Level
embryo 00-02hr
embryo 02-04hr
embryo 04-06hr
embryo 06-08hr
embryo 08-10hr
embryo 10-12hr
embryo 12-14hr
embryo 14-16hr
embryo 16-18hr
embryo 18-20hr
embryo 20-22hr
embryo 22-24hr
larva L1
larva L2
larva L3 12hr old
larva L3 puffstage 1-2
larva L3 puffstage 3-6
larva L3 puffstage 7-9
white prepupae new
white prepupae 12hr
white prepupae 24hr
pupae 2d postWPP
pupae 3d postWPP
pupae 4d postWPP
adult male 01day
adult male 05day
adult male 30day
adult female 01day
adult female 05day
adult female 30day

modENCODE Temporal Expression Data (Graveley et al., 2011)

modENCODE Cell Lines RNA-Seq

modENCODE Cell Line Expression Data

(modENCODE_mRNA-Seq_cell.A)

(modENCODE_mRNA-Seq_cell.B)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		49
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		(49)
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		49
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		49
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		49
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		(49)
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		49
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Cell Line		Expression Level
Schneider line 2 S2R+		14
Schneider line 2 Sg4		7
embryonic 1182-4H		11
embryonic GM2		12
embryonic Kc167		10
embryonic S1		49
embryonic S3		7
leg disc CME L1		10
wing disc CME-W2		10
wing disc ML-DmD8		15
wing disc ML-DmD9		14
wing disc ML-DmD16-c3		13
wing disc ML-DmD21		9
wing disc ML-DmD32		5
haltere disc ML-DmD17-c3		17
eye-antennal disc ML-DmD11		16
antennal disc ML-DmD20-c5		9
mixed discs ML-DmD4-c1		13
CNS ML-DmBG1-c1		14
CNS ML-DmBG2-c2		17
tumorous blood cells mbn2		9
ovary fGS/OSS		18
ovary OSC		16
ovary OSS		11
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Cell Line		Expression Level
Schneider line 2 S2R+
Schneider line 2 Sg4
embryonic 1182-4H
embryonic GM2
embryonic Kc167
embryonic S1
embryonic S3
leg disc CME L1
wing disc CME-W2
wing disc ML-DmD8
wing disc ML-DmD9
wing disc ML-DmD16-c3
wing disc ML-DmD21
wing disc ML-DmD32
haltere disc ML-DmD17-c3
eye-antennal disc ML-DmD11
antennal disc ML-DmD20-c5
mixed discs ML-DmD4-c1
CNS ML-DmBG1-c1
CNS ML-DmBG2-c2
tumorous blood cells mbn2
ovary fGS/OSS
ovary OSC
ovary OSS

modENCODE Treatments RNA-Seq

modENCODE Treatment Expression Data

(modENCODE_mRNA-Seq_treatments)

	Styles
	Linear
	Logarithmic
	Heatmap
	Scales
	gene max expression
	Moderate expression bin max
	High expression bin max
	Extremely high expression bin max

[download data (TSV)]

Guide to modENCODE expression level colors
	No/Extremely low expression (0 - 0)
	Very low expression (1 - 3)
	Low expression (4 - 10)
	Moderate expression (11 - 25)
	Moderately high expression (26 - 50)
	High expression (51 - 100)
	Very high expression (101 - 1000)
	Extremely high expression (>1000)

Linear, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		42
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		46
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High

Linear, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		(44)
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		(42)
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(46)
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		(34)
Paraquat 10 mM 48 hrs, 4-day adult		(28)
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high

Linear, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		42
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		46
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

Linear, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		42
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		46
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Extremely high

log, scaled to maximum expression level
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		42
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		46
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High

log, scaled to Moderate expression
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		(44)
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		(42)
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		(46)
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		(34)
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high

log, scaled to High expression
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		42
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		46
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high

log, scaled to Extremely high expression
Treatment		Expression Level
extended cold, 4-day adult		22
cold shock, 4-day adult		17
heat shock, 4-day adult		17
Cadmium 50 mM 6 hrs, larvae L3		7
Cadmium 50 mM 12 hrs, larvae L3		12
Cadmium 50 mM 48 hrs, 4-day adult		20
Cadmium 100 mM 48 hrs, 4-day adult		44
Copper 0.5 mM 12 hrs, larvae L3		10
Copper 15 mM 48 hrs, 4-day adult		24
Zinc 5 mM 12 hrs, larvae L3		5
Zinc 4.5 mM 48 hrs, 4-day adult		14
Ethanol 2.5% 3 hrs, larvae L3		42
Ethanol 5% 3 hrs, larvae L3		6
Ethanol 10% 3 hrs, larvae L3		8
Caffeine 1.5 mg/ml 4 hrs, larvae L3		7
Caffeine 2.5 mg/ml 48 hrs, 4-day adult		46
Caffeine 25 mg/ml 48 hrs, 4-day adult		15
Paraquat 5 mM 48 hrs, 4-day adult		34
Paraquat 10 mM 48 hrs, 4-day adult		28
Rotenone 2 μg 12 hrs, larvae L3		9
Rotenone 8 μg 12 hrs, larvae L3		7
Expression Level Scale		Very low Low Moderate Moderately high High Very high Extremely high

Heatmap
Treatment		Expression Level
extended cold, 4-day adult
cold shock, 4-day adult
heat shock, 4-day adult
Cadmium 50 mM 6 hrs, larvae L3
Cadmium 50 mM 12 hrs, larvae L3
Cadmium 50 mM 48 hrs, 4-day adult
Cadmium 100 mM 48 hrs, 4-day adult
Copper 0.5 mM 12 hrs, larvae L3
Copper 15 mM 48 hrs, 4-day adult
Zinc 5 mM 12 hrs, larvae L3
Zinc 4.5 mM 48 hrs, 4-day adult
Ethanol 2.5% 3 hrs, larvae L3
Ethanol 5% 3 hrs, larvae L3
Ethanol 10% 3 hrs, larvae L3
Caffeine 1.5 mg/ml 4 hrs, larvae L3
Caffeine 2.5 mg/ml 48 hrs, 4-day adult
Caffeine 25 mg/ml 48 hrs, 4-day adult
Paraquat 5 mM 48 hrs, 4-day adult
Paraquat 10 mM 48 hrs, 4-day adult
Rotenone 2 μg 12 hrs, larvae L3
Rotenone 8 μg 12 hrs, larvae L3

Expression Clusters

A cluster of genes with similar mRNA expression dynamics across development.

(The modENCODE Consortium, 2010)

mE2_34_mRNA_expression_cluster_30

External Data & Images

Linkouts

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

•

FBgn0025186

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

•

CG5709-RA

Alleles & Phenotypes

Summary of Allele Phenotypes

Lethality

Allele

lethal | larval stage | recessive

ari-2⁰⁷⁷⁶⁸

lethal | pupal stage

ari-2⁰⁷⁷⁶⁸

lethal | recessive

ari-2⁰⁷⁷⁶⁸

viable

ari-2^EY00574

Sterility

Allele

fertile

ari-2^EY00574

Other Phenotypes

Allele

increased cell death

ari-2⁰⁷⁷⁶⁸

neuroanatomy defective

ari-2⁰⁷⁷⁶⁸

neuroanatomy defective | somatic clone

ari-2⁰⁷⁷⁶⁸

Phenotype manifest in

Allele

neuroblast | somatic clone

ari-2⁰⁷⁷⁶⁸

Classical Alleles ( 15 )

For All Classical Alleles Show

Allele of ari-2	Mutagen	Stocks	Known lesion
ari-2⁰⁷⁷⁶⁸	P-element activity	1	Yes
ari-2^EY00574	P-element activity	1	--
ari-2^01D01W-L015		0	--
ari-2^01D01W-L063		0	--
ari-2^01D01W-L074		0	--
ari-2^01D01W-L087		0	--
ari-2^01D01W-L093		0	--
ari-2^01D01W-L101		0	--
ari-2^01D01W-L128		0	--
ari-2^01D01W-L165		0	--
ari-2^01D01W-L168		0	--
ari-2^01D01W-L208		0	--
ari-2^EP2128	P-element activity	0	--
ari-2^EP967	P-element activity	0	--
ari-2^Or	P-element activity	0	--

Alleles Carried on Transgenic Constructs ( 2 )

For All Alleles Carried on Transgenic Constructs Show

Allele of ari-2	Class	Mutagen	Stocks	Known lesion
ari-2^GD11743		in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes
ari-2^NIG.5709R		in vitro construct - RNAi in vitro construct - regulatory fusion	0	Yes

Aneuploid Aberrations

Disrupted in

Df(2R)02311

(Spradling et al., 1999)

Df(2R)Exel7171

(Ryder, 2004.4.26)

Not disrupted in

Df(2R)02311

(BDGP Project Members, 1994-1999)

Transgenic Constructs & Insertions

Transgenic Constructs

Type of construct

Name

Expression data

UAS construct

P{GD11743}

P{NIG.5709R}

Insertions

Type of insertions

Name

Expression data

insertion of mobile activating element

P{EP}ari-2^EP967

P{EP}ari-2^EP2128

P{EPgy2}ari-2^EY00574

insertion of enhancer trap

P{PZ}ari-2⁰⁷⁷⁶⁸

miscellaneous insertions

P{5'wHy}ari-2^01D01W-L015

P{5'wHy}ari-2^01D01W-L063

P{5'wHy}ari-2^01D01W-L074

P{5'wHy}ari-2^01D01W-L087

P{5'wHy}ari-2^01D01W-L093

P{5'wHy}ari-2^01D01W-L101

P{5'wHy}ari-2^01D01W-L128

P{5'wHy}ari-2^01D01W-L165

P{5'wHy}ari-2^01D01W-L168

P{5'wHy}ari-2^01D01W-L208

P{(loxP.w.-110+276)w}ari-2^Or

P{wHy}CG11291^DG29403

Gene Ontology: Function, Process & Cellular Component ( 4 unique terms )

Terms Based on Experimental Evidence ( 0 terms )

Terms Based on Predictions or Assertions ( 4 terms )

Molecular Function

CV term

Evidence

References

nucleic acid binding

inferred from electronic annotation with InterPro:IPR001878

(FlyBase Curators et al., 2004-)

ubiquitin-protein ligase activity

non-traceable author statement

(Swiss-Prot Project Members, 2001.8.20)

zinc ion binding

inferred from electronic annotation with InterPro:IPR001841, InterPro:IPR001878, InterPro:IPR002867

(FlyBase Curators et al., 2004-)

Biological Process ( 0 terms)

Cellular Component

CV term

Evidence

References

cytoplasm

non-traceable author statement

(Swiss-Prot Project Members, 2001.8.20)

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Interactions & Pathways

Summary of Physical Interactions

Summary of Genetic Interactions

Interacts with

Please look at the allele data for full details of the genetic interactions

ari-2 allele

Gene

References

External Data

Linkouts

BioGRID - A database of protein and genetic interactions

•

63159

DroID - A comprehensive database of gene and protein interactions.

•

FBgn0025186

InterologFinder Protein-protein interactions (PPI) from both known and predicted PPI data sets.

•

37542

Orthologs

OrthoDB Orthologs (40) - based on analysis using Dmel annotation version 5.41

OrthoDB Ortholog Groups

OrthoDB orthology group searches

(Waterhouse et al., 2011, Nucleic Acids Res. 39(Database issue): D283--D288)

Drosophila inclusive ortholog search

EOG61VKNP

Dipteran inclusive ortholog search

EOG6QJRWM

Insect inclusive ortholog search

EOG6JDG32

Arthropod inclusive ortholog search

EOG68KPSF

Metazoa inclusive ortholog search

EOG6ZD5XR

Orthologs in Drosophila Species (EOG61VKNP)

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila melanogaster

fruit fly

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19075

Drosophila persimilis

Dper\GL17530

Drosophila willistoni

Dwil\GK20805

Drosophila virilis

Dvir\GJ15094

Drosophila mojavensis

Dmoj\GI19927

Drosophila grimshawi

Dgri\GH21451

Orthologs in non-Drosophila Dipterans (EOG6QJRWM)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL007426

Aedes aegypti

Yellow fever mosquito

Aaeg\AAEL009509

Anopheles gambiae

Malaria mosquito

Agam\AGAP005977

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ010461

Culex quinquefasciatus

Southern house mosquito

Cqui\CPIJ016790

Orthologs in non-Dipteran Insects (EOG6JDG32)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Apis mellifera

Western honey bee

Amel\GB47427

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG029180

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG032091

Acromyrmex echinatior

Panamanian leafcutter ant

Aech\AECH18275

Atta cephalotes

Leafcutter ant

Acep\ACEP19166

Camponotus floridanus

Florida carpenter ant

Cflo\CFLO12654

Harpegnathos saltator

Jerdons jumping ant

Hsal\HSAL13487

Linepithema humile

Argentine ant

Lhum\LH11684

Pogonomyrmex barbatus

Red harvester ant

Pbar\PB19768

Solenopsis invicta

Red fire ant

Sinv\SINV21017

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002342

Bombyx mori

Silkmoth

Bmor\BGIBMGA003483

Tribolium castaneum

Red flour beetle

Tcas\TC001697

Orthologs in non-Insect Arthropods (EOG68KPSF)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Daphnia pulex

Water flea

Dpux\JGI_V11_226424

Ixodes scapularis

Deer tick

Isca\ISCW015774

Orthologs in non-Arthropod Metazoa (EOG6ZD5XR)

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Caenorhabditis elegans

Nematode

Cele\T12E12.1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\SPU_022630gn

Danio rerio

Zebrafish

Drer\arih2

Xenopus tropicalis

Western clawed frog

Xtro\arih2

Gallus gallus

Domestic chicken

Ggal\ARIH2

Mus musculus

House mouse

Mmus\Arih2

Rattus norvegicus

Norway rat

Rnor\Arih2

Homo sapiens

Human

Hsap\ARIH2

Human Orthologs (1)

Gene

OMIM

HGNC

Hsap\ARIH2

AAA Orthologs (11) based on analysis using Dmel annotation version 4.3

Organism

Gene

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA19075

Drosophila persimilis

Dper\GL17530

Drosophila willistoni

Dwil\GK20805

Drosophila virilis

Dvir\GJ15094

Drosophila mojavensis

Dmoj\GI19927

Drosophila grimshawi

Dgri\GH21451

Stocks & Reagents

Stocks Listed in FlyBase ( 2 )

Bloomington

12341

P{PZ}ari-2⁰⁷⁷⁶⁸ cn¹/CyO; ry⁵⁰⁶

20092

y¹ w^67c23; P{EPgy2}ari-2^EY00574

Genomic Clones ( 1 )

BACR02O12

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones ( 33 )

Please Note

This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA Clones, Fully Sequenced

BDGP DGC clones