FlyBase Gene Report: Dmel\CycT

General Information

Symbol

Dmel\CycT

Species

D. melanogaster

Name

Cyclin T

Annotation Symbol

CG6292

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025455

Gene Model Status

Current

Stock Availability

13 publicly available

Gene Summary

Cyclin T (CycT) encodes a gene that, together with Cdk9, form the Positive Transcription Elongation Factor b (P-TEFb) complex that is needed to release RNA polymerase II into productive transcription elongation. [Date last reviewed: 2024-01-25] (FlyBase Gene Snapshot)

All Summaries

Gene Snapshot
Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

P-TEFb, Dmcyclin T

Key Links

Genomic Location

Cytogenetic map

74D4-74D4

Sequence location

3L:17,551,004..17,557,788 [-]

Recombination map

3-45

RefSeq locus

NT_037436 REGION:17551004..17557788

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

Gene Ontology (GO) Annotations (16 terms)

Molecular Function (2 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

enables cyclin-dependent protein serine/threonine kinase regulator activity

inferred from direct assay

(Peng et al., 1998)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

enables cyclin-dependent protein serine/threonine kinase activator activity

inferred from biological aspect of ancestor with PANTHER:PTN000002653

(GO Reference Genome Project, 2011-)

enables cyclin-dependent protein serine/threonine kinase regulator activity

inferred from electronic annotation with InterPro:IPR043198

(InterPro Project Members, 2004-)

Biological Process (7 terms)

Terms Based on Experimental Evidence (4 terms)

CV Term

Evidence

References

involved_in DNA-templated transcription

inferred from direct assay

(Lis et al., 2000)

involved_in positive regulation of transcription elongation by RNA polymerase II

inferred from direct assay

(Peng et al., 1998)

involved_in response to heat

inferred from direct assay

(Lis et al., 2000)

involved_in ventral cord development

inferred from high throughput mutant phenotype

(Bivik et al., 2015)

Terms Based on Predictions or Assertions (3 terms)

CV Term

Evidence

References

involved_in positive regulation of DNA-templated transcription, elongation

inferred from biological aspect of ancestor with PANTHER:PTN000002653

(GO Reference Genome Project, 2011-)

involved_in positive regulation of transcription by RNA polymerase II

inferred from biological aspect of ancestor with PANTHER:PTN000002653

(GO Reference Genome Project, 2011-)

involved_in regulation of transcription by RNA polymerase II

inferred from electronic annotation with InterPro:IPR043198

(InterPro Project Members, 2004-)

Cellular Component (7 terms)

Terms Based on Experimental Evidence (6 terms)

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Lis et al., 2000)

part_of P-TEFb complex

inferred from physical interaction with FLYBASE:Cdk9; FB:FBgn0019949

(Peng et al., 1998)

located_in polytene chromosome puff

inferred from direct assay

(Lis et al., 2000)

located_in polytene chromosome

inferred from direct assay

(Lis et al., 2000)

part_of super elongation complex

inferred from physical interaction with FLYBASE:Su(Tpl); FB:FBgn0014037

(Smith et al., 2011)

part_of transcription elongation factor complex

inferred from direct assay

(Smith et al., 2011)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of cyclin/CDK positive transcription elongation factor complex

inferred from biological aspect of ancestor with PANTHER:PTN000002653

(GO Reference Genome Project, 2011-)

traceable author statement

(Lis et al., 2000)

is_active_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000002458

(GO Reference Genome Project, 2011-)

Gene Group (FlyBase)

POSITIVE TRANSCRIPTION ELONGATION FACTOR COMPLEX B

Protein Family (UniProt)

Belongs to the cyclin family. Cyclin C subfamily. (O96433)

Protein Signatures (InterPro)

Summaries

Gene Snapshot

Gene Group (FlyBase)

POSITIVE TRANSCRIPTION ELONGATION FACTOR COMPLEX B -: The positive transcription elongation factor complex b (P-TEFb) is a cyclin dependent kinase complex required for the transition from abortive elongation into productive elongation in RNA polymerase II-mediated transcription. (Adapted from FBrf0103005.)

Protein Function (UniProtKB)

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin T) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to production elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNAP II).

(UniProt, O96433)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\CycT for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Structure

Protein 3D structure (Predicted by AlphaFold) (AlphaFold entry O96433)

If you don't see a structure in the viewer, refresh your browser.

Model Confidence:

Very high (pLDDT > 90)
Confident (90 > pLDDT > 70)
Low (70 > pLDDT > 50)
Very low (pLDDT < 50)

AlphaFold produces a per-residue confidence score (pLDDT) between 0 and 100. Some regions with low pLDDT may be unstructured in isolation.

Experimentally Determined Structures

Crossreferences

Comments on Gene Model

Low-frequency RNA-Seq exon junction(s) not annotated.

Gene model reviewed during 5.46

Gene model reviewed during 5.56

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

CycT-RA

FBtr0075206

NM_206395

3267

884

CycT-RB

FBtr0075205

NM_079403

4305

1097

CycT-RC

FBtr0333124

NM_001275063

5810

1097

CycT-RD

FBtr0333125

NM_001275064

6041

884

Additional Transcript Data and Comments

Reported size (kB)

7.0, 5.5, 5.0 (northern blot)

(Peng et al., 1998)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

UniProt

RefSeq ID

GenBank

CycT-PA

FBpp0089275

94.6

884

10.29

FBpp0074968

118.3

1097

10.08

FBpp0305335

118.3

1097

10.08

FBpp0305336

94.6

884

10.29

M9PI89

NP_001261993

AGB94686

Polypeptides with Identical Sequences

The group(s) of polypeptides indicated below share identical sequence to each other.

1097 aa isoforms: CycT-PB, CycT-PC

884 aa isoforms: CycT-PA, CycT-PD

Additional Polypeptide Data and Comments

Reported size (kDa)

1097 (aa); 118 (kD predicted)

(Peng et al., 1998)

124 (kD observed)

(Marshall and Price, 1995)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the super elongation complex (SEC), at least composed of Ell, Cdk9, cyclin-T (CycT), lilli and ear. Associates with CDK9 to form P-TEFb.

(UniProt, O96433)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\CycT using the Feature Mapper tool.

External Data

Crossreferences

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

Linkouts

Expression Data

Testis-specificity index

The testis specificity index was calculated from modENCODE tissue expression data by Vedelek et al., 2018 to indicate the degree of testis enrichment compared to other tissues. Scores range from -2.52 (underrepresented) to 5.2 (very high testis bias).

0.93

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

anterior endoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

dorsal ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

organism | gap expression pattern

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral ectoderm anlage

Comment: anlage in statu nascendi

(Fisher et al., 2012)

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

Additional Descriptive Data

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in nucleus

inferred from direct assay

(Lis et al., 2000)

part_of P-TEFb complex

inferred from physical interaction with FLYBASE:Cdk9; FB:FBgn0019949

(Peng et al., 1998)

located_in polytene chromosome puff

inferred from direct assay

(Lis et al., 2000)

located_in polytene chromosome

inferred from direct assay

(Lis et al., 2000)

part_of super elongation complex

inferred from physical interaction with FLYBASE:Su(Tpl); FB:FBgn0014037

(Smith et al., 2011)

part_of transcription elongation factor complex

inferred from direct assay

(Smith et al., 2011)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

JBrowse - Visual display of RNA-Seq signals

View Dmel\CycT in JBrowse

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\CycT

View exonic expression by tissue for Dmel\CycT

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025455

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39961/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025455

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025455

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025455

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6292

Flygut - An atlas of the Drosophila adult midgut

FBgn0025455

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\CycT

Stages(s) 1-3
Stages(s) 4-6
Stages(s) 9-10

FlyBase Wiki

Image Based Resources

Alleles, Insertions, Transgenic Constructs, and Aberrations

Classical and Insertion Alleles ( 9 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 13 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of CycT

Transgenic constructs containing regulatory region of CycT

Aberrations (Deficiencies and Duplications) ( 2 )

Inferred from experimentation ( 2 )

Gene disrupted in

Df(3L)Exel9005

(Ryder, 2004.4.26)

Df(3R)ED4688

(Keleman et al., 2005)

Inferred from location ( 8 )

Variants

Variant Molecular Consequences

Alleles Representing Disease-Implicated Variants

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

CycT^j11B2

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

CycT^GD4130

lethal - all die during embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

CycT^HMS00776

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

CycT^GD4130

partially lethal, with Scer\GAL4^tin.CΔ4

CycT^GD4130

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

CycT^GD4130

Other Phenotypes

Allele

abnormal cell shape | blastoderm stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

CycT^HMS00776

abnormal developmental rate, with Scer\GAL4^btl.PS

CycT^HMS00776

abnormal developmental rate | embryonic stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

CycT^HMS00776

abnormal neuroanatomy

CycT^11A005

decreased cell number | blastoderm stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

CycT^HMS00776

decreased cell number | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

CycT^KK100386

increased cell number | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

CycT^{nanos.3'UTR.UASp}

increased fecundity | dominant

CycT^j11B2

some die during pupal stage, with Scer\GAL4^pnr-MD237

CycT^GD4130

Phenotype manifest in

Allele

apterous-expressing neuron of the lateral cluster of the thorax

CycT^11A005

apterous-expressing neuron of the lateral cluster of the thorax Ap4

CycT^11A005

embryo | gastrula stage | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

CycT^HMS00776

embryonic/larval dorsal tracheal branch | larval stage, with Scer\GAL4^btl.PS

CycT^HM04074

female germline cell | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

CycT^{nanos.3'UTR.UASp}

female germline stem cell | oogenesis, with Scer\GAL4^{VP16.nanos.UTR}

CycT^KK100386

germarium, with Scer\GAL4^{VP16.nanos.UTR}

mesoderm | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

CycT^HMS00776

Orthologs

Downloads

Download All DIOPT Orthologs

Human Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Homo sapiens (Human) (12)

Hsap\CCNT1

12 of 14

Yes

Hsap\CCNT2

12 of 14

Yes

3 of 14

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

1 of 14

1 of 14

Model Organism Orthologs (via DIOPT v9.1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Rattus norvegicus (Norway rat) (9)

Rnor\Ccnt2

12 of 14

Yes

Rnor\Ccnt1

10 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Yes

Mus musculus (laboratory mouse) (8)

Mmus\Ccnt1

11 of 14

Yes

Mmus\Ccnt2

11 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

Xenopus tropicalis (Western clawed frog) (15)

Xtro\ccnt2

7 of 13

Yes

Xtro\ccnt1

2 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Yes

Danio rerio (Zebrafish) (10)

Drer\ccnt1

12 of 14

Yes

Drer\ccnt2a

12 of 14

Yes

Drer\ccnt2b

11 of 14

Yes

3 of 14

3 of 14

3 of 14

2 of 14

2 of 14

2 of 14

1 of 14

Caenorhabditis elegans (Nematode, roundworm) (7)

Cele\cit-1.1

9 of 14

Yes

Cele\cit-1.2

7 of 14

Yes

3 of 14

2 of 14

2 of 14

1 of 14

1 of 14

Anopheles gambiae (African malaria mosquito) (8)

Agam\AgaP_AGAP006678

9 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

2 of 12

1 of 12

1 of 12

Arabidopsis thaliana (thale-cress) (14)

Atha\CYCT1;3

6 of 13

Yes

5 of 13

5 of 13

5 of 13

5 of 13

2 of 13

2 of 13

2 of 13

2 of 13

1 of 13

1 of 13

1 of 13

1 of 13

1 of 13

Saccharomyces cerevisiae (Brewer's yeast) (4)

Scer\CTK2

3 of 13

Yes

Scer\CCL1

2 of 13

Scer\SSN8

2 of 13

Scer\BUR2

1 of 13

Schizosaccharomyces pombe (Fission yeast) (5)

Spom\pch1

4 of 12

Yes

3 of 12

2 of 12

2 of 12

2 of 12

Escherichia coli (enterobacterium) (0)

Other Organism Orthologs (via OrthoDB)

Data provided directly from OrthoDB:CycT. Refer to their site for version information.

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v9.1)

Gene Symbol

Score

Source

Alignment

Drosophila melanogaster (Fruit fly) (7)

6 of 13

5 of 13

4 of 13

4 of 13

3 of 13

1 of 13

1 of 13

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

CycT^j11B2

ameliorates tauopathy

modeled by Hsap\MAPT^GMR.Ex.PJ

(Ambegaokar and Jackson, 2011)

Disease Associations of Human Orthologs (via DIOPT v9.1 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

12 of 14

12 of 14

3 of 14

3 of 14

INTELLECTUAL DEVELOPMENTAL DISORDER WITH HYPERTELORISM AND DISTINCTIVE FACIES; IDDHDF

3 of 14

3 of 14

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Show neighbor-neighbor interactions:

Off

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

Select Layout:

Force Directed

Circle

Concentric

Legend:

Protein

RNA

Selected Interactor(s)

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

CycT - CG17601

experimental knowledge based

(Guruharsha et al., 2011)

CycT - Cdk9

ion exchange chromatography, cosedimentation through density gradient, molecular weight estimation by staining, experimental knowledge based, anti tag coimmunoprecipitation, western blot, peptide massfingerprinting, pull down, two hybrid

(Kim et al., 2013, Guruharsha et al., 2011, Hanyu-Nakamura et al., 2008, Peng et al., 1998, Zhu et al., 1997, Marshall et al., 1996, Marshall and Price, 1995)

CycT - Hexim

anti bait coimmunoprecipitation, western blot

(Nguyen et al., 2021, Nguyen et al., 2012)

CycT - Larp7

anti bait coimmunoprecipitation, western blot

(Nguyen et al., 2021, Nguyen et al., 2012)

CycT - Su(Tpl)

anti tag coimmunoprecipitation, peptide massfingerprinting

(Smith et al., 2011)

CycT - pgc

anti tag coimmunoprecipitation, western blot, pull down

(Hanyu-Nakamura et al., 2008)

RNA-protein

Physical Interaction

Assay

References

CycT - snRNA:7SKa

anti bait coimmunoprecipitation, quantitative reverse transcription pcr, northern blot

(Nguyen et al., 2021, Nguyen et al., 2012)

Summary of Genetic Interactions

esyN Network Diagram

Show/hide secondary interactors
(data from AllianceMine provided by esyN)

esyN Network Key:

Suppression

Enhancement

Other Interaction Browsers

View in FlyBase Interactions Browser View in MIST tool (external link)

Please look at the allele data for full details of the genetic interactions

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

bin3

suppressible

CycT

(Palumbo et al., 2024)

trc

enhanceable

CycT

(Hudson et al., 2023)

trc

suppressible

CycT

(Hudson et al., 2023)

External Data

Subunit Structure (UniProtKB)

Component of the super elongation complex (SEC), at least composed of Ell, Cdk9, cyclin-T (CycT), lilli and ear. Associates with CDK9 to form P-TEFb.

(UniProt, O96433 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65249

DroID - A comprehensive database of gene and protein interactions.

FBgn0025455

MIST (protein-protein) - An integrated Molecular Interaction Database

39961

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

FlyBase

External Links

External Data

Linkouts

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Viral life cycle - HIV-1

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O96433

Class of Gene

nuclear_gene

protein_coding_gene

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-45

Cytogenetic map

74D4-74D4

Sequence location

3L:17,551,004..17,557,788 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

74D4-74D4

Limits computationally determined from genome sequence between P{PZ}frc⁰⁰⁰⁷³&P{PZ}frc⁰²⁶¹⁹ and P{lacW}l(3)j11B2^j11B2

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

74E-74F

(Peng, 1998.3.2)

Experimentally Determined Recombination Data

Location

3-45

(FlyBase, 2016)

3-41.0

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (13)

Aberrations including deletions of this gene Aberrations including duplications of this gene

Bloomington

31762

y¹ v¹; P{TRiP.HM04074}attP2

91965

y¹ v¹; P{TKO.GS00031}attP40

37678

y¹ w^*; Mi{MIC}CycT^MI04468

35168

y¹ sc^* v¹ sev²¹; P{TRiP.GL00037}attP2

79935

y¹ sc^* v¹ sev²¹; P{TOE.GS02216}attP40

32976

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00776}attP2

Kyoto

125610

w¹¹¹⁸; P{RS5}CycT^5-HA-2393

NIG-Fly

6292R-3

P{NIG.6292R}3

HMS00776

y¹ sc^* v¹ sev²¹; P{TRiP.HMS00776}attP2

VDRC

v103387

P{KK100386}VIE-260B

v37562

w¹¹¹⁸; P{GD4130}v37562/CyO

v342511

P{hsFLP}1, y¹ w¹¹¹⁸; P{HD_CFD01937}attP40/CyO-GFP

More stocks available...

Genomic Clones (17)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (73)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see JBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

FI21261

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025455

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025455

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Nguyen et al., 2012, Peng et al., 1998)

Commercially Available Antibodies

Cell Line Information

Publicly Available Cell Lines

Other Stable Cell Lines

Other Comments

Identified as a potential component of the hh signalling pathway in a genome-wide RNAi screen. dsRNA made from templates generated with primers directed affects the extent of expression of a hh signaling reporter construct in Clone 8 cells.

(Nybakken et al., 2005)

RNAi generated by PCR using primers directed to this gene causes a cell growth and viability phenotype when assayed in Kc167 and S2R⁺ cells.

(Boutros et al., 2004)

dsRNA made from templates generated with primers directed against this gene tested in RNAi screen for effects on Kc167 and S2R⁺ cell morphology.

(Kiger et al., 2003)

RNAi screen using dsRNA made from templates generated with primers directed against this gene causes a phenotype when assayed in Kc167 cells: change from round to spindle-shaped, with the formation of F-actin puncta and microtubule extensions. Alterations of cell shape are also evident in S2R+ cells. In both Kc167 and S2R⁺ cells, cell morphology is aberrant and there is an increased frequency of microtubule-based mitotic spindles, indicative of a failure in mitosis.

(Kiger et al., 2003)

CycT encodes the large subunit of P-TEFb (positive transcription elongation factor b).

(Peng et al., 1998)

Identification: 6.0kb transcript identified during molecular analysis of the Eip74EF genomic region.

(Burtis et al., 1990)

Relationship to Other Genes

Source for database merge of

Source for merge of: CycT anon-74EFc

(FlyBase, 1996-)

Additional comments

Nomenclature History

Source for database identify of

Nomenclature comments

Etymology

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

CG6292

(Hosono et al., 2015, Ni et al., 2011.7.19, Ni et al., 2010.12.1, Ardehali et al., 2009, Keleman et al., 2009.8.5, Ni et al., 2008, Maia et al., 2007, Nybakken et al., 2005)

CycT

(Hunt et al., 2024, Lee et al., 2024, Sood et al., 2024, Hudson et al., 2023, Riemondy et al., 2023, Lebo et al., 2021, Rosendo Machado et al., 2021, Li et al., 2020, Hanyu-Nakamura et al., 2019, Flora et al., 2018, Housden et al., 2017, Liu et al., 2017, Transgenic RNAi Project members, 2017-, Zanet et al., 2016, Bivik et al., 2015, Dahlberg et al., 2015, Di Cara et al., 2015, Housden et al., 2015, Vedanayagam and Garrigan, 2015, Appocher et al., 2014, Gaertner and Zeitlinger, 2014, Gonzalez et al., 2014, Handler et al., 2013, Kwon et al., 2013, Schaaf et al., 2013, Japanese National Institute of Genetics, 2012.5.21, Tokusumi et al., 2012, Zraly and Dingwall, 2012, Guo et al., 2011, Smith et al., 2011, Zobeck et al., 2010, Ardehali et al., 2009, Bakal et al., 2008, Hanyu-Nakamura et al., 2008, Srinivasan et al., 2008, Ivaldi et al., 2007, Maia et al., 2007, Nybakken et al., 2005)

Dmcyclin T

(Peng et al., 1998)

ORE-14

(Maia et al., 2007)

P-TEFb

(Zobeck et al., 2010, Zobeck et al., 2010, Hanyu-Nakamura et al., 2008, Srinivasan et al., 2008, Saunders et al., 2005, Garriga and Grana, 2004, Sims et al., 2004, Boehm et al., 2003, Peng et al., 1998, Yamaguchi et al., 1998, Zhu et al., 1997, Marshall et al., 1996, Marshall and Price, 1995)

anon-74EFc

(Fu et al., 1999)

p124

(Yamaguchi et al., 1998)

Name Synonyms

Cyclin T

(Kim et al., 2013, Nguyen et al., 2012, Zhang et al., 2008, Peng et al., 1998)

CyclinT

(Ni et al., 2004)

Dmcyclin T

(Peng et al., 1998)

cyclin T

(Schwartz et al., 2003, Peng, 1998.3.2)

cyclin T1

(Zorio and Bentley, 2001)

cyclin T2

(Zorio and Bentley, 2001)

dCyclin T

(Bartkowiak et al., 2010)

dmCyclin T

(Nguyen et al., 2021)

Secondary FlyBase IDs

FBgn0023101

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

Study result (0)

Result

Result Type

Title

External Crossreferences and Linkouts ( 63 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39961

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/GCRP - The gene-centric reference proteome (GCRP) provides a 1:1 mapping between genes and UniProt accessions in which a single 'canonical' isoform represents the product(s) of each protein-coding gene.

O96433

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O96433

UniProt/TrEMBL - Automatically annotated and unreviewed records of protein sequence and functional information

Other crossreferences

AlphaFold DB - AlphaFold provides open access to protein structure predictions for the human proteome and other key proteins of interest, to accelerate scientific research.

O96433

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025455

DRscDB - A single-cell RNA-seq resource for data mining and data comparison across species

39961/tissue=All

EMBL-EBI Single Cell Expression Atlas - Single cell expression across species

FBgn0025455

FlyAtlas2 - A Drosophila melanogaster expression atlas with RNA-Seq, miRNA-Seq and sex-specific data

FBgn0025455

FlyMine - An integrated database for Drosophila genomics

FBgn0025455

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

dme:Dmel_CG6292

MARRVEL_MODEL - MARRVEL (model organism gene)

39961

Linkouts

BioGRID - A database of protein and genetic interactions.

65249

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0025455

DroID - A comprehensive database of gene and protein interactions.

FBgn0025455

DRSC - Results frm RNAi screens

FBgn0025455

Eukaryotic Promoter Database - A collection of databases of experimentally validated promoters for selected model organisms.

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

FBgn0025455

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0025455

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG6292

Flygut - An atlas of the Drosophila adult midgut

FBgn0025455

FlyMet - A comprehensive tissue-specific metabolomics resource for Drosophila.

FBgn0025455

iBeetle-Base - RNAi phenotypes in the red flour beetle (Tribolium castaneum)

TC031193

KEGG Pathways - A collection of manually drawn pathway maps representing knowledge of molecular interaction, reaction and relation networks.

Viral life cycle - HIV-1

MIST (protein-protein) - An integrated Molecular Interaction Database

39961

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

SignaLink - A signaling pathway resource with multi-layered regulatory networks.

O96433

References (112)

The following external sites may use different assemblies or annotations than FlyBase.

0.93

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