FlyBase Gene Report: Dmel\eIF3e

General Information

Symbol

Dmel\eIF3e

Species

D. melanogaster

Name

eukaryotic translation initiation factor 3 subunit e

Annotation Symbol

CG9677

Feature Type

protein_coding_gene

FlyBase ID

FBgn0025582

Gene Model Status

Current

Stock Availability

4 publicly available

Gene Snapshot

eukaryotic translation initiation factor 3 subunit e (eIF3e) encodes a component of the translation initiation complex and is a positive regulator of cullin neddylation, which is required for Cullin-ring ubiquitin ligase activity. It also regulates spindle assembly, microtubule dynamics and mitotic progression. [Date last reviewed: 2018-10-18]

Other Summaries

Alliance
Auto summary
Gene Group
UniProtKB
Interactive Fly

Also Known As

Int6, l(3)10547, eIF3-S6, Int6 homologue

Key Links

Genomic Location

Cytogenetic map

73C4-73C4

Sequence location

3L:16,785,242..16,786,941 [-]

Recombination map

3-44

RefSeq locus

NT_037436 REGION:16785242..16786941

Sequence

Get Decorated FASTA

Genomic Maps

JBrowse GBrowse

Help me choose

Other Genome Views

The following external sites may use different assemblies or annotations than FlyBase.

Function

GO Summary Ribbons

Gene Ontology (GO) Annotations (5 terms)

Molecular Function (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

enables translation initiation factor activity

inferred from sequence or structural similarity with HGNC:3277

(Miyazaki et al., 1999)

inferred from sequence or structural similarity

(Lasko, 2000)

Biological Process (1 term)

Terms Based on Experimental Evidence (0 terms)

Terms Based on Predictions or Assertions (1 term)

CV Term

Evidence

References

involved_in translational initiation

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from biological aspect of ancestor with PANTHER:PTN000035120

(assigned by GO_Central )

(Gaudet et al., 2011)

inferred from sequence or structural similarity with HGNC:3277

(Miyazaki et al., 1999)

Cellular Component (3 terms)

Terms Based on Experimental Evidence (1 term)

CV Term

Evidence

References

located_in intracellular membrane-bounded organelle

inferred from direct assay

(Miyazaki et al., 1999)

Terms Based on Predictions or Assertions (2 terms)

CV Term

Evidence

References

part_of eukaryotic translation initiation factor 3 complex

inferred from sequence or structural similarity

(Lasko, 2000)

inferred from sequence or structural similarity with HGNC:3277

(Miyazaki et al., 1999)

inferred from biological aspect of ancestor with PANTHER:PTN000035120

(assigned by GO_Central )

(Gaudet et al., 2011)

located_in nucleus

inferred from biological aspect of ancestor with PANTHER:PTN000035120

(assigned by GO_Central )

(Gaudet et al., 2011)

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS

Protein Family (UniProt)

Belongs to the eIF-3 subunit E family. (O77410)

Protein Signatures (InterPro)

Summaries

Gene Group (FlyBase)

CYTOPLASMIC TRANSLATION INITIATION FACTORS -: Cytoplasmic (or eukaryotic) translation initiation factors function in the initiation stage of ribosome-mediated translation in the cytoplasm. (Adapted from FBrf0233552 and PMID:20094052).

Protein Function (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation (PubMed:18493598). In addition to its role in the eIF-3 complex, also functions in protein ubiquitination and degradation (PubMed:18493598, PubMed:28505193). During mitosis required for regulating mitotic microtubule growth and kinetochore formation, and consequently is required for satisfying the spindle assembly checkpoint (SAC) during metaphase to prevent delays in mitotic progression (PubMed:28505193). This is likely by promoting the ubiquitination and degradation of Klp67A, a kinesin-like protein that suppresses microtubule polymerization at plus ends (PubMed:28505193). Acts in the COP9 signalosome (CSN) mediated regulation of cullin neddylation by promoting Cul1 and Cul3 neddylation and negatively regulating the CSN complex subunit CSN5 (PubMed:18493598).

(UniProt, O77410)

Gene Model and Products

Number of Transcripts

Number of Unique Polypeptides

Please see the JBrowse view of Dmel\eIF3e for information on other features

To submit a correction to a gene model please use the Contact FlyBase form

Protein Domains (via Pfam)

Isoform displayed:

Pfam protein domains

InterPro name

classification

start

end

Protein Domains (via SMART)

Isoform displayed:

SMART protein domains

InterPro name

classification

start

end

Comments on Gene Model

Gene model reviewed during 5.46

Sequence Ontology: Class of Gene

nuclear_gene

protein_coding_gene

Transcript Data

Annotated Transcripts

Name

FlyBase ID

RefSeq ID

Length (nt)

Assoc. CDS (aa)

eIF3e-RA

FBtr0075345

NM_058037

1520

435

Additional Transcript Data and Comments

Reported size (kB)

1.5 (northern blot)

(Miyazaki et al., 1999)

Comments

External Data

Crossreferences

Polypeptide Data

Annotated Polypeptides

Name

FlyBase ID

Predicted MW (kDa)

Length (aa)

Theoretical pI

RefSeq ID

GenBank

eIF3e-PA

FBpp0075104

51.2

435

6.77

NP_477385

AAF49412

Polypeptides with Identical Sequences

There is only one protein coding transcript and one polypeptide associated with this gene

Additional Polypeptide Data and Comments

Reported size (kDa)

435 (aa); 48 (kD observed)

(Miyazaki et al., 1999)

Comments

External Data

Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt (PubMed:23716590).

(UniProt, O77410)

Crossreferences

InterPro - A database of protein families, domains and functional sites

Linkouts

Sequences Consistent with the Gene Model

Nucleotide / Polypeptide Records

UniProt

O77410

Mapped Features

Click to get a list of regulatory features (enhancers, TFBS, etc.) and gene disruptions (point mutations, indels, etc.) within or overlapping Dmel\eIF3e using the Feature Mapper tool.

External Data

Crossreferences

Linkouts

Expression Data

Expression Summary Ribbons

Colored tiles in ribbon indicate that expression data has been curated by FlyBase for that anatomical location. Colorless tiles indicate that there is no curated data for that location.

For complete stage-specific expression data, view the modENCODE Development RNA-Seq section under High-Throughput Expression below.

Transcript Expression

in situ

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage 1 -- 3

organism

Comment: maternally deposited

(Fisher et al., 2012)

embryonic stage 4 -- 6

organism | ubiquitous

(Fisher et al., 2012)

embryonic stage 7 -- 8

anterior endoderm anlage

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 9 -- 10

anterior endoderm

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior endoderm

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

embryonic stage 11 -- 12

anterior midgut primordium

(Fisher et al., 2012)

central brain primordium

(Fisher et al., 2012)

head mesoderm

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

posterior midgut primordium

(Fisher et al., 2012)

trunk mesoderm

(Fisher et al., 2012)

ventral nerve cord primordium

(Fisher et al., 2012)

embryonic stage 13 -- 16

embryonic brain

(Fisher et al., 2012)

embryonic/larval midgut

(Fisher et al., 2012)

organism | faint | ubiquitous

(Fisher et al., 2012)

organism | ubiquitous

(Fisher et al., 2012)

ventral nerve cord

(Fisher et al., 2012)

northern blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Miyazaki et al., 1999)

Additional Descriptive Data

Int6 transcripts are detected at all developmental stages tested. Levels are particularly high in early embryos.

(Miyazaki et al., 1999)

Marker for

Subcellular Localization

CV Term

Polypeptide Expression

mass spectroscopy

Stage

Tissue/Position (including subcellular localization)

Reference

day 7 of adulthood -- day 49 of adulthood

adult heart

(Cammarato et al., 2011)

adult stage

adult head

• membrane

(Aradska et al., 2015)

western blot

Stage

Tissue/Position (including subcellular localization)

Reference

embryonic stage -- adult stage

(Miyazaki et al., 1999)

Additional Descriptive Data

Int6 protein is detected at all developmental stages tested. Levels are particularly high in early embryos.

(Miyazaki et al., 1999)

Marker for

Subcellular Localization

CV Term

Evidence

References

located_in intracellular membrane-bounded organelle

inferred from direct assay

(Miyazaki et al., 1999)

Expression Deduced from Reporters

High-Throughput Expression Data

Associated Tools

GBrowse - Visual display of RNA-Seq signals

View Dmel\eIF3e in GBrowse 2

RNA-Seq by Region - Search RNA-Seq expression levels by exon or genomic region

View exonic expression by developmental stage for Dmel\eIF3e

View exonic expression by tissue for Dmel\eIF3e

Bulk Downloads

RNA-Seq RPKM values for all genes

Reference

See Gelbart and Emmert, 2013 for analysis details and data files for all genes.

Developmental Proteome: Life Cycle

Developmental Proteome: Embryogenesis

External Data and Images

Linkouts

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025582

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0025582

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9677-RA

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9677

Flygut - An atlas of the Drosophila adult midgut

FBgn0025582

Images

FlyExpress - Embryonic expression images (BDGP data)

Show all FlyExpress processed images for Dmel\eIF3e

Stages(s) 4-6
Stages(s) 7-8
Stages(s) 9-10
Stages(s) 11-12
Stages(s) 13-16

FlyBase Wiki

Image Based Resources

Alleles, Insertions, and Transgenic Constructs

Classical and Insertion Alleles ( 4 )

For All Classical and Insertion Alleles Show

Other relevant insertions

Transgenic Constructs ( 4 )

For All Alleles Carried on Transgenic Constructs Show

Transgenic constructs containing/affecting coding region of eIF3e

Transgenic constructs containing regulatory region of eIF3e

Deletions and Duplications ( 10 )

Disrupted in

Df(3L)81k19

(Spradling et al., 1999, Cunniff et al., 1997, BDGP Project Members, 1994-1999)

Df(3L)ED223

(Marygold et al., 2007)

Df(3L)ED4684

(Marygold et al., 2007)

Df(3L)Exel6130

(Marygold et al., 2007, Ryder, 2004.4.26)

Df(3L)st-b11

(Cunniff et al., 1997)

In(3L)std11

(Cunniff et al., 1997)

Not disrupted in

Df(3L)st4

(Cunniff et al., 1997)

Df(3L)st100.62

(Cunniff et al., 1997)

Df(3L)st-g24

(Cunniff et al., 1997)

Df(3L)st-j7

(Spradling et al., 1999, Cunniff et al., 1997, BDGP Project Members, 1994-1999)

Phenotypes

For more details about a specific phenotype click on the relevant allele symbol.

Lethality

Allele

lethal | recessive

lethal - all die before end of first instar larval stage | recessive

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

eIF3e^GD14286

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

eIF3e^GD14286

partially lethal, with Scer\GAL4^tin.CΔ4

eIF3e^GD14286

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

eIF3e^GD14286

Other Phenotypes

Allele

decreased cell number | somatic clone | third instar larval stage

eIF3e^A69

developmental rate defective | first instar larval stage | recessive

some die during first instar larval stage | recessive

some die during pupal stage, with Scer\GAL4^pnr-MD237

eIF3e^GD14286

Phenotype manifest in

Allele

embryonic/larval brain | somatic clone | third instar larval stage

eIF3e^A69

eye disc | somatic clone

eIF3e¹⁰⁵⁴⁷

female germline cell | germline clone

eIF3e¹⁰⁵⁴⁷

wing disc | somatic clone

eIF3e¹⁰⁵⁴⁷

Orthologs

Downloads

Download All DIOPT Orthologs

Download All OrthoDB Orthologs

Human Orthologs (via DIOPT v8.0)

Homo sapiens (Human) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Hsap\EIF3E

14 of 15

Yes

Model Organism Orthologs (via DIOPT v8.0)

Mus musculus (laboratory mouse) (1)

Species\Gene Symbol

Score

Best Score

Best Reverse Score

Source

Alignment

Complementation?

Transgene?

Mmus\Eif3e

13 of 15

Yes

Rattus norvegicus (Norway rat) (5)

Rnor\Eif3el1

8 of 13

Yes

Rnor\LOC108348260

7 of 13

Yes

Rnor\LOC100365062

2 of 13

Yes

Rnor\LOC100909481

2 of 13

Yes

Rnor\Eif3e

1 of 13

Yes

Xenopus tropicalis (Western clawed frog) (1)

Xtro\eif3e

13 of 12

Yes

Danio rerio (Zebrafish) (2)

Drer\eif3eb

13 of 15

Yes

Drer\eif3ea

12 of 15

Yes

Caenorhabditis elegans (Nematode, roundworm) (1)

Cele\eif-3.E

13 of 15

Yes

Arabidopsis thaliana (thale-cress) (1)

Atha\EIF3E

9 of 9

Yes

Saccharomyces cerevisiae (Brewer's yeast) (0)

No records found.

Schizosaccharomyces pombe (Fission yeast) (1)

Spom\int6

11 of 12

Yes

Ortholog(s) in Drosophila Species (via OrthoDB v9.1) ( EOG091907ME )

Organism

Common Name

Gene

AAA Syntenic Ortholog

Multiple Dmel Genes in this Orthologous Group

Drosophila suzukii

Spotted wing Drosophila

Drosophila simulans

Drosophila sechellia

Drosophila erecta

Drosophila yakuba

Drosophila ananassae

Drosophila pseudoobscura pseudoobscura

Dpse\GA21959

Drosophila persimilis

Dper\GL16190

Drosophila willistoni

Dwil\GK22114

Drosophila willistoni

Dwil\GK12672

Drosophila virilis

Dvir\GJ12157

Drosophila mojavensis

Dmoj\GI11931

Drosophila grimshawi

Dgri\GH15221

Orthologs in non-Drosophila Dipterans (via OrthoDB v9.1) ( EOG091504G9 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Musca domestica

House fly

Mdoa\17017556

Glossina morsitans

Tsetse fly

Gmor\GMOY009287

Lucilia cuprina

Australian sheep blowfly

Lcup\FF38_07530

Mayetiola destructor

Hessian fly

Mdes\Mdes000108

Aedes aegypti

Yellow fever mosquito

Aaeg\eIF3-S6

Anopheles darlingi

American malaria mosquito

Adar\ADAC001582

Orthologs in non-Dipteran Insects (via OrthoDB v9.1) ( EOG090W04CD )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Bombyx mori

Silkmoth

Bmor\BGIBMGA003669

Danaus plexippus

Monarch butterfly

Dple\DPOGS215609-PA

Heliconius melpomene

Postman butterfly

Hmel\HMEL005811

Apis florea

Little honeybee

Aflo\3399506900

Apis mellifera

Western honey bee

Amel\GB53916

Bombus impatiens

Common eastern bumble bee

Bimp\BIMP25236

Bombus terrestris

Buff-tailed bumblebee

Bter\15205341

Linepithema humile

Argentine ant

Lhum\LH22413

Megachile rotundata

Alfalfa leafcutting bee

Mrot\MROTU:0020f9

Nasonia vitripennis

Parasitic wasp

Nvit\Nasvi2EG016846

Dendroctonus ponderosae

Mountain pine beetle

Dpod\YQE_12325

Tribolium castaneum

Red flour beetle

Tcas\TC002738

Pediculus humanus

Human body louse

Phum\PHUM042220

Rhodnius prolixus

Kissing bug

Rpro\RPRC004935

Cimex lectularius

Bed bug

Clec\CLEC008497

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI002661

Acyrthosiphon pisum

Pea aphid

Apim\ACYPI004684

Zootermopsis nevadensis

Nevada dampwood termite

Znev\Znev_04124

Orthologs in non-Insect Arthropods (via OrthoDB v9.1) ( EOG090X0491 )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strigamia maritima

European centipede

Smar\SMAR013786

Ixodes scapularis

Black-legged tick

Isca\ISCW023004

Stegodyphus mimosarum

African social velvet spider

Smim\KFM67912.1

Tetranychus urticae

Two-spotted spider mite

Turt\tetur23g00430

Daphnia pulex

Water flea

Dpux\DAPPUDRAFT_302804

Orthologs in non-Arthropod Metazoa (via OrthoDB v9.1) ( EOG091G075K )

Organism

Common Name

Gene

Multiple Dmel Genes in this Orthologous Group

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Eif3e_1

Strongylocentrotus purpuratus

Purple sea urchin

Spur\Sp-Eif3e

Ciona intestinalis

Vase tunicate

Cint\LOC100185058

Gallus gallus

Domestic chicken

Ggal\EIF3E

Paralogs

Downloads

Download All DIOPT Paralogs

Paralogs (via DIOPT v8.0)

Drosophila melanogaster (Fruit fly) (0)

No records found.

Human Disease Associations

FlyBase Human Disease Model Reports

Disease Model Summary Ribbon

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Allele

Disease

Evidence

References

Potential Models Based on Orthology ( 0 )

Human Ortholog

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Allele

Disease

Interaction

References

eIF3e¹⁰⁵⁴⁷

ameliorates autosomal dominant cerebellar ataxia

modeled by Hsap\ATXN8OS^CTG112.UAS

(Mutsuddi et al., 2004)

Disease Associations of Human Orthologs (via DIOPT v8.0 and OMIM)

Note that ortholog calls supported by only 1 or 2 algorithms (DIOPT score < 3) are not shown.

Homo sapiens (Human)

Gene name

Score

OMIM

OMIM Phenotype

DO term

Complementation?

Transgene?

EIF3E; eukaryotic translation initiation factor 3 subunit E

14 of 15

602210

Functional Complementation Data

Functional complementation data is computed by FlyBase using a combination of the orthology data obtained from DIOPT and OrthoDB and the allele-level genetic interaction data curated from the literature.

Interactions

Summary of Physical Interactions

esyN Network Diagram

Interactions Browser

View in Interactions Browser

Please see the Physical Interaction reports below for full details

protein-protein

Physical Interaction

Assay

References

eIF3e - Lkb1

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3e - Pten

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3e - S6kII

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3e - eIF3a

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3b

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3c

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3d1

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3f1

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3g1

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3h

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3k

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3l

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - eIF3m

experimental knowledge based

(Guruharsha et al., 2011)

eIF3e - foxo

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3e - gig

anti tag coimmunoprecipitation, Identification by mass spectrometry

(Vinayagam et al., 2016)

eIF3e - mxt

anti tag coimmunoprecipitation, anti tag western blot

(Hernández et al., 2013)

Summary of Genetic Interactions

esyN Network Diagram

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

Starting gene(s)

Interaction type

Interacting gene(s)

Reference

External Data

Subunit Structure (UniProtKB)

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex. The eIF-3 complex interacts with pix. Interacts with mxt (PubMed:23716590).

(UniProt, O77410 )

Linkouts

BioGRID - A database of protein and genetic interactions.

65176

DroID - A comprehensive database of gene and protein interactions.

FBgn0025582

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

39877

MIST (protein-protein) - An integrated Molecular Interaction Database

Int6

Pathways

Signaling Pathways (FlyBase)

Metabolic Pathways

External Data

Linkouts

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Genomic Location and Detailed Mapping Data

Chromosome (arm)

Recombination map

3-44

Cytogenetic map

73C4-73C4

Sequence location

3L:16,785,242..16,786,941 [-]

FlyBase Computed Cytological Location

Cytogenetic map

Evidence for location

73C4-73C4

Limits computationally determined from genome sequence between P{PZ}Baldspot⁰²²⁸¹ and P{PZ}blot⁰¹⁶⁵⁸

Experimentally Determined Cytological Location

Cytogenetic map

Notes

References

73D1-73D2

(determined by in situ hybridisation)

(Spradling et al., 1999, BDGP Project Members, 1994-1999)

73C2-73C2

(determined by in situ hybridisation)

(Miyazaki et al., 1999)

Experimentally Determined Recombination Data

Location

3-44

(FlyBase, 2016)

3-39.4

(Comeron et al., 2012)

Left of (cM)

Right of (cM)

Notes

Stocks and Reagents

Stocks (4)

Bloomington

59072

w^*; P{UAS-eIF3e.GFP}12

59073

w^*; Kr^If-1/CyO; P{UAS-eIF3e.Frag.GFP}31

VDRC

v103559

P{KK101123}VIE-260B

v27032

w¹¹¹⁸; P{GD14286}v27032

Genomic Clones (13)

Please Note FlyBase no longer curates genomic clone accessions so this list may not be complete

cDNA Clones (245)

Please Note This section lists cDNAs and ESTs that fall within the genomic extent of the gene model, which may include cDNAs and ESTs of genes within introns, or of overlapping genes. Please see GBrowse for alignment of the cDNAs and ESTs to the gene model.

cDNA clones, fully sequenced

BDGP DGC clones

Other clones

Drosophila Genomics Resource Center cDNA clones

For each fully sequenced cDNA the DGRC maintains various forms of the cDNA (e.g tagged or untagged) in several different host vectors for subsequent cloning and expression in Drosophila and Drosophila cell lines.

FBgn0025582

cDNA Clones, End Sequenced (ESTs)

BDGP DGC clones

Other clones

RNAi and Array Information

Linkouts

DRSC - Results frm RNAi screens

FBgn0025582

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39877

Antibody Information

Laboratory Generated Antibodies

polyclonal

(Renda et al., 2017, Miyazaki et al., 1999)

Commercially Available Antibodies

Other Information

Relationship to Other Genes

Source for database identify of

Source for identity of: eIF3e Int6

(Marygold et al., 2017)

Source for database merge of

Source for merge of: Int6 l(3)10547

(Spradling et al., 1999)

Additional comments

Other Comments

S2 cells treated with dsRNA generated against this gene show reduced phagocytosis of Candida albicans compared to untreated cells.

(Stroschein-Stevenson et al., 2006)

Origin and Etymology

Discoverer

Etymology

Identification

External Crossreferences and Linkouts ( 41 )

Sequence Crossreferences

NCBI Gene - Gene integrates information from a wide range of species. A record may include nomenclature, Reference Sequences (RefSeqs), maps, pathways, variations, phenotypes, and links to genome-, phenotype-, and locus-specific resources worldwide.

39877

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.

RefSeq - A comprehensive, integrated, non-redundant, well-annotated set of reference sequences including genomic, transcript, and protein.

UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information

O77410

Other crossreferences

BDGP expression data - Patterns of gene expression in Drosophila embryogenesis

FBgn0025582

Drosophila Genomics Resource Center - Drosophila Genomics Resource Center (DGRC) cDNA clones

FBgn0025582

Fly-FISH - A database of Drosophila embryo and larvae mRNA localization patterns

CG9677

Flygut - An atlas of the Drosophila adult midgut

FBgn0025582

GenomeRNAi - A database for cell-based and in vivo RNAi phenotypes and reagents

39877

InterPro - A database of protein families, domains and functional sites

KEGG Genes - Molecular building blocks of life in the genomic space.

Dmel_CG9677

modMine - A data warehouse for the modENCODE project

FBgn0025582

Linkouts

BioGRID - A database of protein and genetic interactions.

65176

DroID - A comprehensive database of gene and protein interactions.

FBgn0025582

DRSC - Results frm RNAi screens

FBgn0025582

FLIGHT - Cell culture data for RNAi and other high-throughput technologies

FBgn0025582

FlyAtlas - Adult expression by tissue, using Affymetrix Dros2 array

CG9677-RA

FlyCyc Genes - Genes from a BioCyc PGDB for Dmel

FBGN0025582

FlyMine - An integrated database for Drosophila genomics

FBgn0025582

InterologFinder - Protein-protein interactions (PPI) from both known and predicted PPI data sets.

39877

KEGG Pathways - Wiring diagrams of molecular interactions, reactions and relations.

RNA transport

MIST (protein-protein) - An integrated Molecular Interaction Database

Int6

Reactome - An open-source, open access, manually curated and peer-reviewed pathway database.

Synonyms and Secondary IDs (13)

Reported As

Symbol Synonym

BcDNA.GM01233

(Tsang, 1999.3.2)

BcDNA:GM01233

CG9677

(Marygold et al., 2017, Hosono et al., 2015, Fukuoh et al., 2014, Hernández et al., 2013, Cammarato et al., 2011, Keleman et al., 2009.8.5, Sepp et al., 2008, Lasko, 2000)

DINT6

(Miyazaki et al., 1999)

Int6

(Jin et al., 2020, Richardson, 2015.3.11, Ashwal-Fluss et al., 2014, Friedman et al., 2011, Guest et al., 2011, White et al., 2011, Andersen et al., 2010, Parry et al., 2010, Rencus-Lazar et al., 2008, Sepp et al., 2008, Somma et al., 2008, Marygold et al., 2007, Slack et al., 2006, Stroschein-Stevenson et al., 2006)

eIF-3p48/INT-6

(Miyazaki et al., 1999)

eIF3-S6

(Parry et al., 2010, Somma et al., 2008, Lasko, 2000)

eIF3-p48/INT6

(Traicoff and Callahan, 2000)

eIF3e

(Rode et al., 2018, Marygold et al., 2017, Majzoub et al., 2014, Hernández et al., 2013)

l(3)10547

(Beaton, 2000.1.31, Cunniff et al., 1997, Meister and Braun, 1995.10, BDGP Project Members, 1994-1999)

l(3)j9E8

Name Synonyms

Int6

Int6 homologue

(Cammarato et al., 2011, Spradling et al., 1999)

eukaryotic translation initiation factor 3 subunit e

Secondary FlyBase IDs

FBgn0010945
FBgn0011424

Datasets (0)

Study focus (0)

Experimental Role

Project

Project Type

Title

References (65)

The following external sites may use different assemblies or annotations than FlyBase.

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